| 1. |
- Hägglund, Maria G A, et al.
(författare)
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B(0)AT2 (SLC6A15) is localized to neurons and astrocytes, and is involved in mediating the effect of leucine in the brain
- 2013
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 8:3, s. e58651-
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Tidskriftsartikel (refereegranskat)abstract
- The B(0)AT2 protein is a product of the SLC6A15 gene belonging to the SLC6 subfamily and has been shown to be a transporter of essential branched-chain amino acids. We aimed to further characterize the B(0)AT2 transporter in CNS, and to use Slc6a15 knock out (KO) mice to investigate whether B(0)AT2 is important for mediating the anorexigenic effect of leucine. We used the Slc6a15 KO mice to investigate the role of B(0)AT2 in brain in response to leucine and in particular the effect on food intake. Slc6a15 KO mice show lower reduction of food intake as well as lower neuronal activation in the ventromedial hypothalamic nucleus (VMH) in response to leucine injections compared to wild type mice. We also used RT-PCR on rat tissues, in situ hybridization and immunohistochemistry on mouse CNS tissues to document in detail the distribution of SLC6A15 on gene and protein levels. We showed that B(0)AT2 immunoreactivity is mainly neuronal, including localization in many GABAergic neurons and spinal cord motor neurons. B(0)AT2 immunoreactivity was also found in astrocytes close to ventricles, and co-localized with cytokeratin and diazepam binding inhibitor (DBI) in epithelial cells of the choroid plexus. The data suggest that B(0)AT2 play a role in leucine homeostasis in the brain.
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| 2. |
- Roshanbin, Sahar, et al.
(författare)
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PAT4 is abundantly expressed in excitatory and inhibitory neurons as well as epithelial cells
- 2014
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Ingår i: Brain Research. - : Elsevier BV. - 0006-8993 .- 1872-6240. ; 1557, s. 12-25
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Tidskriftsartikel (refereegranskat)abstract
- PAT4, the fourth member of the SLC36/proton dependent amino acid transporter (PAT) family, is a high-affinity, low capacity electroneutral transporter of neutral amino acids like proline and tryptophan. It has also been associated with the function of mTORC1, a complex in the mammalian target of rapamycin (mTOR) pathway. We performed in situ hybridization and immunohistological analysis to determine the expression profile of PAT4, as well as an RT-PCR study on tissue from mice exposed to leucine. We performed a phylogenetic analysis to determine the evolutionary origin of PAT4. The in situ hybridization and the immunohistochemistry on mouse brain sections and hypothalamic cells showed abundant PAT4 expression in the mouse brain intracellularly in both inhibitory and excitatory neurons, partially co-localizing with lysosomal markers and epithelial cells lining the ventricles. Its location in epithelial cells around the ventricles indicates a transport of substrates across the blood brain barrier. Phylogenetic analysis showed that PAT4 belongs to an evolutionary old family most likely predating animals, and PAT4 is the oldest member of that family.
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| 3. |
- Wang, Yinan, et al.
(författare)
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On first-order compensation of timing mismatch in two-channel TIADCs
- 2020
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Ingår i: 2020 IEEE INTERNATIONAL SYMPOSIUM ON CIRCUITS AND SYSTEMS (ISCAS). - : IEEE. - 9781728133201
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Konferensbidrag (refereegranskat)abstract
- In this paper, two first-order compensation strategies for timing mismatch in two-channel time-interleaved analog-to-digital converters (TIADCs) are analyzed, and expressions for the spurious-free dynamic range (SFDR) after compensation are derived. The derived expressions reveal that the strategy where both channels are compensated to match each other, using half the value of the mismatch with different signs, achieves a substantially greater SFDR than the strategy where only one channel is compensated to match the other (reference) channel. This is because, after compensation, the remaining aliasing distortion is shown to be of third order in the former strategy whereas it is of second order in the latter. Simulations included demonstrate the validity of the derived expressions.
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| 4. |
- Zhu, Yuqing, 1998, et al.
(författare)
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Single-Layer Dual-Polarized End-Fire Phased Array Antenna for Low-Cost Millimeter-Wave Mobile Terminal Applications
- 2022
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Ingår i: IEEE Conference on Antenna Measurements and Applications, CAMA. - 2474-1760. ; 2022-December
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Konferensbidrag (refereegranskat)abstract
- A dual-polarized end-fire phased array antenna printed on a single-layer substrate is successfully developed for low-cost millimeter-wave mobile terminal applications. Metallic strips loaded in front of the substrate integrated waveguide improve the performance of the vertical polarization. The gradual grounded coplanar waveguide and V-shaped parasitic dipoles achieve compact horizontally polarized elements. Both polarizations are combined to establish an array with an element spacing of 6.3 mm. The proposed phased array realizes a -10 dB impedance bandwidth of 7.9% and a port isolation above 15 dB, capable of covering the n261 band. The realized gain is greater than 9 dBi for both polarizations, with a beam scanning range of about 79°.
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