| 1. |
- Ali, Ashfaq, et al.
(författare)
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Paranoid potato : phytophthora-resistant genotype shows constitutively activated defense
- 2012
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Ingår i: Plant Signaling and Behavior. - : Informa UK Limited. - 1559-2316 .- 1559-2324. ; 7:3, s. 400-408
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Tidskriftsartikel (refereegranskat)abstract
- Phytophthora is the most devastating pathogen of dicot plants. There is a need for resistance sources with different modes of action to counteract the fast evolution of this pathogen. In order to better understand mechanisms of defense against P. infestans, we analyzed several clones of potato. Two of the genotypes tested, Sarpo Mira and SW93-1015, exhibited strong resistance against P. infestans in field trials, whole plant assays and detached leaf assays. The resistant genotypes developed different sizes of hypersensitive response (HR)-related lesions. HR lesions in SW93-1015 were restricted to very small areas, whereas those in Sarpo Mira were similar to those in Solanum demissum, the main source of classical resistance genes. SW93-1015 can be characterized as a cpr (constitutive expressor of PR genes) genotype without spontaneous microscopic or macroscopic HR lesions. This is indicated by constitutive hydrogen peroxide (H₂O₂) production and PR1 (pathogenesis-related protein 1) secretion. SW93-1015 is one of the first plants identified as having classical protein-based induced defense expressed constitutively without any obvious metabolic costs or spontaneous cell death lesions.
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| 2. |
- Zoteyeva, Nadezhda, et al.
(författare)
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Late blight and virus host-plant resistances, crossing ability and glycoalkaloids in Nordic potato germplasm
- 2017
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Ingår i: Acta Agriculturae Scandinavica, Section B - Soil and Plant Science. - : Informa UK Limited. - 0906-4710 .- 1651-1913. ; 67, s. 628-636
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Tidskriftsartikel (refereegranskat)abstract
- Old potato cultivars from the Nordic Genetic Resource Center (NordGen) and advanced breeding clones from the Swedish University of Agricultural Sciences (SLU) were evaluated for susceptibility to Potato Virus Y (PVY) and Potato Leafroll Virus (PLRV), foliar and tuber resistance to late blight - caused by Phytophthora infestans - as well as for glycoalkaloid content and crossing ability. Enzyme-Linked Immunosorbant Assay (ELISA) tests were used for PVY and PLRV screening during two years that were characterized by intensive virus incidence and severity in the field, while foliar and tuber resistances to late blight were assessed under artificial inoculation with an aggressive Swedish isolate of P. infestans. Hybrid seeds were obtained by crossing cultivars such as Kiva', Sarpo Mira', Rosamunda' and Superb' with SLU advanced breeding clones and a selected clone of Solanum tuberosum Group Andigena. Some cultivars (Harek', Sarpo Mira') and breeding clones (04-2662, 04-2085) with late blight resistance did not show virus infection. The -solanin and -chaconin contents of some of the old Nordic potato cultivars and breeding clones were similar to the known Dutch table cultivar Bintje' after 3 years of testing. This research allows identification of promising Nordic potato cultivars and SLU breeding clones for further use in developing germplasm aiming at organic and conventional farming systems.
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