| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Charpentier, Sophie, 1983, et al.
(författare)
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Induced unconventional superconductivity on the surface states of Bi2Te3 topological insulator
- 2017
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Ingår i: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723 .- 2041-1723. ; 8:1
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Tidskriftsartikel (refereegranskat)abstract
- Topological superconductivity is central to a variety of novel phenomena involving the interplay between topologically ordered phases and broken-symmetry states. The key ingredient is an unconventional order parameter, with an orbital component containing a chiral p(x) + ip(y) wave term. Here we present phase-sensitive measurements, based on the quantum interference in nanoscale Josephson junctions, realized by using Bi2Te3 topological insulator. We demonstrate that the induced superconductivity is unconventional and consistent with a sign-changing order parameter, such as a chiral px + ipy component. The magnetic field pattern of the junctions shows a dip at zero externally applied magnetic field, which is an incontrovertible signature of the simultaneous existence of 0 and pi coupling within the junction, inherent to a non trivial order parameter phase. The nano-textured morphology of the Bi2Te3 flakes, and the dramatic role played by thermal strain are the surprising key factors for the display of an unconventional induced order parameter.
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| 3. |
- Chen, Xiren, et al.
(författare)
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Bi-Induced Electron Concentration Enhancement Being Responsible for Photoluminescence Blueshift and Broadening in InAs Films
- 2019
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Ingår i: Physica Status Solidi (B): Basic Research. - : Wiley. - 1521-3951 .- 0370-1972. ; 256:5
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Tidskriftsartikel (refereegranskat)abstract
- Photoluminescence (PL) study is conducted on InAs films molecular beam epitaxially grown on GaAs substrates with different Bi flux levels. A PL peak blueshift accompanied by linewidth broadening is found with the increase of Bi/As flux ratio, in contrast to the common Bi isoelectronic incorporation or surfactant effect. It is, with detailed lineshape analysis and the evidence of PL peak splitting in a magnetic filed, attributed to the electron concentration enhancement induced by Bi flux. The electron concentration in InAs film is evaluated, which is about 5-fold enhanced as Bi/As flux ratio rises up from 0 to 1x10(-3). The temperature dependence of the PL spectrum indicates that the carrier redistribution augments while the carrier-phonon Frohlich scattering weakens in InAs films with high Bi/As flux ratios. These findings reveal a novel Bi effect of electron concentration enhancement, and contribute to the basic knowledge of Bi in III-V semiconductors.
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| 4. |
- Chen, X, et al.
(författare)
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Shallow-terrace-like interface in dilute-bismuth GaSb/AlGaSb single quantum wells evidenced by photoluminescence
- 2013
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Ingår i: Journal of Applied Physics. - : AIP Publishing. - 0021-8979 .- 1089-7550. ; 113:15, s. 153505-153507
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Tidskriftsartikel (refereegranskat)abstract
- Photoluminescence (PL) measurements are performed on one GaSb/AlGaSb single-quantum-well (SQW) sample and two dilute-bismuth (Bi) GaSb/AlGaSb SQW samples grown at 360 and 380 °C, at low temperatures and under magnetic fields. Bimodal PL features are identified in the dilute-Bi samples, and to be accompanied by abnormal PL blueshift in the sample grown at 360 °C. The bimodal PL features are found to be from similar origins of band-to-band transition by magneto-PL evolution. Analysis indicates that the phenomenon can be well interpreted by the joint effect of interfacial large-lateral-scale islands and Al/Ga interdiffusion due to Bi incorporation. The interdiffusion introduces about 1-monolayer shrinkage to the effective quantum-well thickness, which is similar to the interfacial islands height, and the both together result in an unusual shallow-terrace-like interface between GaSbBi and AlGaSb. A phenomenological model is established, the Bi content of isoelectronic incorporation and the exciton reduced effective mass are estimated for the GaSbBi sample grown at 380 °C, and a value of about 21 meV/% is suggested for the bandgap bowing rate of GaSbBi. An effective routine is suggested for determining the Bi content and the depth of the shallow-terraces at interface in dilute-Bi SQW structures.
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| 5. |
- Chen, Yuqian, et al.
(författare)
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Experimental and numerical study of flow and ignition and lean blowout characteristics of jet-cooled wall flameholder in a dual-mode combustor
- 2022
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Ingår i: Aerospace Science and Technology. - : Elsevier BV. - 1270-9638. ; 122
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Tidskriftsartikel (refereegranskat)abstract
- The wall flameholder is one of the credible alternatives to realize pilot ignition in augmented/ramjet combustors. To overcome the ablation for a long-term operation, two types of jet cooling, external-inhaled air and pressure-driven jet cooling, are proposed for the wall flameholder. In this work, the flow and combustion process in a laboratory scale rig is studied for different cooling schemes and cooling conditions using experimental and numerical methods. Flow analysis in pressure-driven jet cooling scheme shows that the flow field of flameholder is influenced significantly by the cooling hole angle on the oblique plate α and on the rear plate β. In particular, the cooling jet angle combinations (α=30o, β=30o) and (α=90o, β=150o) are the two schemes with the most different characteristics. To investigate the effects of jet cooling type and cooling jet angle on the flow, ignition, and lean blowout (LBO) characteristics, the two distinctively different angle combinations are applied to form two kinds of jet cooling schemes mentioned above. Results suggest that the jet cooling type has less impact on the flow field but more influence on the flow loss than the cooling jet angle. The ignition performance of cooling schemes with α=30o and β=30o is better than that of those with α=90o and β=150o, but it has a more significant flow loss. The LBO limits of external-inhaled air cooling are lower than that of pressure-driven jet cooling. Moreover, the ignition and LBO limits decrease gradually with the increased mainstream temperature and they are only slightly affected by the mainstream velocity. Notably, the pressure-driven jet cooling scheme can slightly reduce the flow loss but it leads to a deteriorated ignition and LBO performance. The external-inhaled air cooling scheme with α=30o and β=30o has an excellent ignition and LBO performance, and the ignition and LBO limits increase with the increasing cooling air flow rate and the decreasing cooling air temperature.
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| 6. |
- Chen, Yuqian, et al.
(författare)
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Experimental study on combustion and flow resistance characteristics of an afterburner with air-cooled bluff-body flameholder
- 2022
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Ingår i: Aerospace Science and Technology. - : Elsevier BV. - 1270-9638. ; 123
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Tidskriftsartikel (refereegranskat)abstract
- In the afterburner assembled with an air-cooled bluff-body flameholder, cooling air is directly injected into the recirculation zone behind the bluff-body, which can reduce the local temperature and increase the oxygen concentration of the gas mixture in the wake of the bluff-body, thereby affecting the total pressure loss and combustion characteristics. To better understand the flow and combustion process of the system, the exhaust gas temperature, cold and hot total pressure losses in a rectangular premixed combustor are investigated under different cooling air jet conditions. Experimental results show that the added cooling air could improve the combustion efficiency and widen the blowout limit, whereas it could also give rise to an extra total pressure loss. However, when the cooling air flow rate was higher than a critical value, i.e., after the blowing ratio reached 2.5, the recirculation zone could be blown away, resulting in a failed ignition in the afterburner. Notably, the decreased temperature difference between the mainstream and the cooling air could improve the combustion efficiency and reduce the thermal resistance loss but enlarge the cold flow loss and hot total pressure loss. Moreover, since the oxygen content declined and autoignition appeared after the mainstream temperature reached 1100 K, the exhaust gas temperature and combustion efficiency declined rapidly, and the hot total pressure loss also decreased. In addition, with the fuel-gas ratio increasing, the combustion efficiency significantly dropped, the exhaust gas temperature and thermal resistance loss firstly increased to a peak value (at the equivalence ratio of 1.14) and then decreased for excessively fuel-rich combustion.
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| 7. |
- Chen, Yuqian, et al.
(författare)
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Investigation of flame characteristics and cooling effectiveness of jet-cooled wall flameholders in vitiated flow
- 2022
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Ingår i: Aerospace Science and Technology. - : Elsevier BV. - 1270-9638. ; 127
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Tidskriftsartikel (refereegranskat)abstract
- Experimental and numerical studies were performed to understand the flame characteristics and cooling effect of jet-cooled wall flameholders in a simplified augmented combustor. The time-averaged flame structure and wall temperature were investigated employing an instantaneous flame capturing system with image processing technology and a wall temperature measurement system, respectively. Flow and fuel distribution simulations were carried out to analyze the flame structure and cooling effect of the jet-cooled flameholders in high-temperature and high-velocity vitiated flows. It was found that the jet cooling type and the cooling jet angle on the oblique plate α and on the rear plate β greatly affect the flame features and cooling effect of the flameholder. The external-inhaled air cooling schemes can increase the flame luminosity and temperature and create a flame hollowed-out zone in the near-wall region to reduce flame radiation and heat convection, thereby achieving an excellent cooling effect. The pressure-driven gas jet cooling schemes yielded a weaker flame luminosity and its flame structure in the cavity more sensitive to the cooling jet angle than external-inhaled air cooling schemes. Moreover, the external-inhaled air cooling schemes have significantly better cooling effects than the pressure-driven gas jet cooling schemes under different mainstream conditions and fuel flow rates. Notably, the cooling schemes with α=30∘ and β=30∘ have more uniform wall temperature than that of α=90∘ and β=150∘ on the above two plates. Furthermore, the external-inhaled air cooling scheme with α=90∘ and β=150∘ has the best cooling performance under different mainstream and cooling air conditions and presents great cooling effectiveness in the case of enhancing cooling air temperature.
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| 8. |
- Chen, Yuqian, et al.
(författare)
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Study on ignition process and flame expansion and propagation characteristics in jet-cooled pilot flameholders using image processing techniques
- 2022
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Ingår i: Aerospace Science and Technology. - : Elsevier BV. - 1270-9638. ; 129
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Tidskriftsartikel (refereegranskat)abstract
- Wall-type pilot flameholder has been used in aero-engines to effectively create a stable ignition source and achieve an excellent ignition performance in high-velocity flows. The ignition delay, flame propagation, and flame boundary are crucial characteristics to evaluate the performance of pilot flameholders. Previous work displayed the significantly changed ignition performance and flow features of the wall flameholder when jet cooling schemes were adopted, indicating that the ignition process and the flame features strongly depend on the cooling jet and the optimal design of jet cooling schemes needs further investigation. Therefore, in this study, two practical image processing algorithms are developed to characterize the flame development of the ignition process, reveal the effect of cooling schemes on the ignition delay time, and quantitatively analyze the flame area and expansion ratio of the wall flameholder during stable combustion. Two image processing methods proposed based on MATLAB algorithms exhibit effectiveness in studying the ignition process and flame expansion characteristics. The ignition process is divided into four phases: kernel generation, kernel propagation, flame growth, and stable combustion. Meanwhile, the ignition delay time is identified by counting the total pixels of the flame projected region during the ignition process. The edge detection and pixel statistics of the flame projected region in the binary luminosity image show the flame boundary and expansion ratio under various working conditions. It was found that the cooling jet angle has a significant impact on the ignition process, ignition delay time, flame propagation, and steady flame features. The pressure-driven gas jet cooling scheme with a smaller jet angle and the external-inhaled air cooling scheme with a larger jet angle present a better capability of ignition and flame expansion and propagation.
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| 9. |
- Fu, Yifeng, 1984, et al.
(författare)
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Templated Growth of Covalently Bonded Three-Dimensional Carbon Nanotube Networks Originated from Graphene
- 2012
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Ingår i: Advanced Materials. - : Wiley. - 0935-9648 .- 1521-4095. ; 24:12, s. 1576-1581
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Tidskriftsartikel (refereegranskat)abstract
- A template-assisted method that enables the growth of covalently bonded three-dimensional carbon nanotubes (CNTs) originating from graphene at a large scale is demonstrated. Atomic force microscopy-based mechanical tests show that the covalently bonded CNT structure can effectively distribute external loading throughout the network to improve the mechanical strength of the material.
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| 10. |
- Fülöp, Attila, 1988, et al.
(författare)
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Phase transition of bismuth telluride thin films grown by MBE
- 2014
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Ingår i: Applied Physics Express. - 1882-0786 .- 1882-0778. ; 7:4, s. Art. no. 045503-
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Tidskriftsartikel (refereegranskat)abstract
- A previously unreported phase transition between Bi2Te3 and Bi4Te3 in bismuth telluride grown by molecular beam epitaxy is recorded via XRD, AFM, and SIMS observations. This transition is found to be related to the Te/Bi beam equivalent pressure (BEP) ratio. BEP ratios below 17 favor the formation of Bi4Te3 while Bi2Te3 is formed at higher ratios. Transport measurements reveal that Bi2Te3 has higher electron mobility than Bi4Te3.
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