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Sökning: WFRF:(Chan Chun Kit)

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1.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2.
  • Feng, Zhenhua, et al. (författare)
  • Digital Domain Power Division Multiplexing DDO-OFDM Transmission with Successive Interference Cancellation
  • 2016
  • Ingår i: 2016 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO). - Washington, D.C. : IEEE conference proceedings.
  • Konferensbidrag (refereegranskat)abstract
    • Two independent 2.5-Gb/s DDO-OFDM signals are simultaneously transmitted over 25km SMF using digital domain power division multiplexing and successive interference cancellation. With optimized power division ratio and enhanced SD-FEC, the spectral efficiency can be doubled.
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3.
  • Lin, Rui, et al. (författare)
  • Physical-layer network coding for passive optical interconnect in datacenter networks
  • 2017
  • Ingår i: Optics Express. - 1094-4087. ; 25:15, s. 17788-17797
  • Tidskriftsartikel (refereegranskat)abstract
    • We introduce physical-layer network coding (PLNC) technique in a passive optical interconnect (POI) architecture for datacenter networks. The implementation of the PLNC in the POI at 2.5 Gb/s and 10Gb/s have been experimentally validated while the gains in terms of network layer performances have been investigated by simulation. The results reveal that in order to realize negligible packet drop, the wavelengths usage can be reduced by half while a significant improvement in packet delay especially under high traffic load can be achieved by employing PLNC over POI.
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  • Resultat 1-3 av 3

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