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Sökning: WFRF:(Cristiglio Viviana)

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1.
  • Gonzalez Rodriguez, Adrian, 1988, et al. (författare)
  • Melting transition of oriented Li-DNA fibers submerged in ethanol solutions
  • 2021
  • Ingår i: Biopolymers. - : Wiley. - 0006-3525 .- 1097-0282. ; 112:3
  • Tidskriftsartikel (refereegranskat)abstract
    • The melting transition of Li-DNA fibers immersed in ethanol-water solutions has been studied using calorimetry and neutron diffraction techniques. The data have been analyzed using the Peyrard-Bishop-Dauxois model to determine the strengths of the intra- and inter-base pair potentials. The data and analysis show that the potentials are weaker than those for DNA in water. They become weaker still and the DNA less stable as the ethanol concentration increases but, conversely, the fibers become more compact and the distances between base pairs become more regular. The results show that the melting transition is relatively insensitive to local confinement and depends more on the interaction between the DNA and its aqueous environment.
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2.
  • Luchini, Alessandra, et al. (författare)
  • Effect of ergosterol on the interlamellar spacing of deuterated yeast phospholipid multilayers
  • 2020
  • Ingår i: Chemistry and Physics of Lipids. - : Elsevier BV. - 0009-3084. ; 227
  • Tidskriftsartikel (refereegranskat)abstract
    • Sterols regulate several physico-chemical properties of biological membranes that are considered to be linked to function. Ergosterol is the main sterol molecule found in the cell membranes of yeasts and other fungi. Like the cholesterol found in mammalian cells, ergosterol has been proposed to have an ordering and condensing effect on saturated phospholipid membranes. The effects of cholesterol have been investigated extensively and result in an increase in the membrane thickness and the lipid acyl chain order. Less information is available on the effects of ergosterol on phospholipid membranes. Neutron Diffraction (ND) was used to characterize the effect of ergosterol on lipid multilayers prepared with deuterated natural phospholipids extracted from the yeast Pichia pastoris. The data show that the effect of ergosterol on membranes prepared from the natural phospholipid extract rich in unsaturated acyl chains, differs from what has been observed previously in membranes rich in saturated phospholipids. In contrast to cholesterol in synthetic phospholipid membranes, the presence of ergosterol up to 30 mol % in yeast phospholipid membranes only slightly altered the multilayer structure. In particular, only a small decrease in the multilayer d-spacing was observed as function of increasing ergosterol concentrations. This result highlights the need for further investigation to elucidate the effects of ergosterol in biological lipid mixtures.
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