| 1. |
- Assenhöj, Maria, et al.
(author)
-
Protein interaction, monocyte toxicity and immunogenic properties of cerium oxide crystals with 5% or 14% gadolinium, cobalt oxide and iron oxide nanoparticles–an interdisciplinary approach
- 2021
-
In: Nanotoxicology. - : Taylor and Francis Ltd.. - 1743-5390 .- 1743-5404. ; 15:8, s. 1035-1038
-
Journal article (peer-reviewed)abstract
- Metal oxide nanoparticles are widely used in both consumer products and medical applications, but the knowledge regarding exposure-related health effects is limited. However, it is challenging to investigate nanoparticle interaction processes with biological systems. The overall aim of this project was to improve the possibility to predict exposure-related health effects of metal oxide nanoparticles through interdisciplinary collaboration by combining workflows from the pharmaceutical industry, nanomaterial sciences, and occupational medicine. Specific aims were to investigate nanoparticle-protein interactions and possible adverse immune reactions. Four different metal oxide nanoparticles; CeOx nanocrystals with 5% or 14% Gd, Co3O4, and Fe2O3, were characterized by dynamic light scattering and high-resolution transmission electron microscopy. Nanoparticle-binding proteins were identified and screened for HLA-binding peptides in silico. Monocyte interaction with nanoparticle–protein complexes was assessed in vitro. Herein, for the first time, immunogenic properties of nanoparticle-binding proteins have been characterized. The present study indicates that especially Co3O4-protein complexes can induce both ‘danger signals’, verified by the production of inflammatory cytokines and simultaneously bind autologous proteins, which can be presented as immunogenic epitopes by MHC class II. The clinical relevance of these findings should be further evaluated to investigate the role of metal oxide nanoparticles in the development of autoimmune disease. The general workflow identified experimental difficulties, such as nanoparticle aggregate formation and a lack of protein-free buffers suitable for particle characterization, protein analyses, as well as for cell studies. This confirms the importance of future interdisciplinary collaborations. © 2021 The Author(s).
|
|
| 2. |
- Björklund, Emil, et al.
(author)
-
Analysis of protein-ligand interactions from titrations and nuclear magnetic resonance relaxation dispersions
- 2022
-
In: Protein Science. - : Wiley. - 0961-8368 .- 1469-896X. ; 31:1, s. 301-307
-
Journal article (peer-reviewed)abstract
- We present PLIS, a publicly available, open-source software for the determination of protein-ligand dissociation constants that can be used to characterize biological processes or to shed light on biophysical aspects of interactions. PLIS can analyze data from titration experiments monitored by for instance fluorescence spectroscopy or from nuclear magnetic resonance relaxation dispersion experiments. In addition to analysis of experimental data, PLIS includes functionality for generation of synthetic data, useful for understanding how different parameters effect the data in order to better analyze experiments.
|
|
| 3. |
- Cortese, Samuele, et al.
(author)
-
The management of sleep disturbances in children with attention-deficit/hyperactivity disorder (ADHD) : an update of the literature
- 2024
-
In: Expert Review of Neurotherapeutics. - : Expert Reviews Ltd.. - 1473-7175 .- 1744-8360. ; 24:6, s. 585-596
-
Research review (peer-reviewed)abstract
- INTRODUCTION: Sleep disorders represent an important comorbidity in individuals with ADHD. While the links between ADHD and sleep disturbances have been extensively investigated, research on the management of sleep disorders in individuals with ADHD is relatively limited, albeit expanding.AREAS COVERED: The authors searched PubMed, Medline, PsycInfo, Embase+Embase Classic, Web of Sciences databases, and clinicaltrials.gov up to 4 January 2024, for randomized controlled trials (RCTs) of any intervention for sleep disorders associated with ADHD. They retained 16 RCTs (eight on pharmacological and eight on non-pharmacological interventions), supporting behavioral intervention and melatonin, and nine ongoing RCTs registered on clinicaltrials.gov. EXPERT OPINION: The pool of RCTs testing interventions for sleep disorders in individuals with ADHD is expanding. However, to inform clinical guidelines, there is a need for additional research in several areas, including 1) RCTs based on a precise phenotyping of sleep disorders; 2) pragmatic RCTs recruiting neurodevelopmental populations representative of those seen in clinical services; 3) trials testing alternative interventions (e.g. suvorexant or light therapy) or ways to deliver them (e.g. online); 4) sequential and longer-term RCTs; 5) studies testing the impact of sleep interventions on outcomes other than sleep; 6) and implementation of advanced evidence synthesis and precision medicine approaches.
|
|
| 4. |
- Du Rietz, Sabina, 1983-, et al.
(author)
-
Consumer behavior as a challenge and opportunity for circular food packaging : A systematic literature review
- 2022
-
Conference paper (other academic/artistic)abstract
- The food sector is a potential site for the implementation of circular economy strategies. Food packaging cause concern due to high production volume, short usage, problems of waste management and littering. Circular economy strategies could address these concerns if there is consumer acceptance and participation in systems with food packaging. We seek to review current knowledge of consumer behavior in relation to circular food packaging. The study finds that circular economy in terms of the recycling strategy, are found foremost in European and American setting. While reuse strategies were more prevalent in studies from 1970-80s, such initiatives have been renewed.
|
|
| 5. |
- Du Rietz, Sabina, 1983-, et al.
(author)
-
Consumer Behavior as a Challenge and Opportunity for Circular Food Packaging : a Systematic Literature Review
- 2024
-
In: Circular Economy and Sustainability. - : Springer. - 2730-597X .- 2730-5988. ; 4, s. 413-438
-
Research review (peer-reviewed)abstract
- The food sector has been targeted as a potential site for the implementation of circular economy strategies. Food packaging, in particular, has caused concern due to its high production volume, short usage time, and problems related to waste management and littering. Circular economy strategies could potentially address these concerns if there is consumer acceptance and participation in circular systems. The present review seeks to inform future studies of the need for further research in this area by reviewing current knowledge of consumer behavior in relation to circular food packaging. When doing so, the study finds that studies have primarily focused on recycling, foremost in the European and American setting. However, less is known about consumers and circular packaging in developing countries. While reuse strategies were more prevalent in studies from the 1970–1980s, the review finds that such initiatives are yet again researched, which is promising given their generally favorable environmental footprint.
|
|
| 6. |
- Eriksson, Peter, et al.
(author)
-
Cerium Oxide Nanoparticles with Entrapped Gadolinium for High T-1 Relaxivity and ROS-Scavenging Purposes
- 2022
-
In: ACS Omega. - : American Chemical Society (ACS). - 2470-1343. ; 7:24, s. 21337-21345
-
Journal article (peer-reviewed)abstract
- Gadolinium chelates are employed worldwide today as clinical contrast agents for magnetic resonance imaging. Until now, the commonly used linear contrast agents based on the rare-earth element gadolinium have been considered safe and well-tolerated. Recently, concerns regarding this type of contrast agent have been reported, which is why there is an urgent need to develop the next generation of stable contrast agents with enhanced spin-lattice relaxation, as measured by improved T-1 relaxivity at lower doses. Here, we show that by the integration of gadolinium ions in cerium oxide nanoparticles, a stable crystalline 5 nm sized nanoparticulate system with a homogeneous gadolinium ion distribution is obtained. These cerium oxide nanoparticles with entrapped gadolinium deliver strong T-1 relaxivity per gadolinium ion (T-1 relaxivity, r(1) = 12.0 mM(-1) s(-1)) with the potential to act as scavengers of reactive oxygen species (ROS). The presence of Ce3+ sites and oxygen vacancies at the surface plays a critical role in providing the antioxidant properties. The characterization of radial distribution of Ce3+ and Ce4+ oxidation states indicated a higher concentration of Ce3+ at the nanoparticle surfaces. Additionally, we investigated the ROS-scavenging capabilities of pure gadolinium-containing cerium oxide nanoparticles by bioluminescent imaging in vivo, where inhibitory effects on ROS activity are shown.
|
|
| 7. |
- Eskilsson, Olof, et al.
(author)
-
Self-Assembly of Metal Nanoparticles in Bacterial Cellulose for the Fabrication of Soft Substrate-Supported Catalysts
- 2024
-
In: ACS Applied Nano Materials. - : AMER CHEMICAL SOC. - 2574-0970.
-
Journal article (peer-reviewed)abstract
- The transition to green and sustainable catalysts necessitates efficient and safe preparation techniques using abundant and renewable resources. Many metal nanoparticles (NPs) are excellent catalysts but suffer from poor colloidal stability. NP immobilization or fabrication of metal nanostructures on solid supports can avoid issues with NP aggregation and facilitate the reuse of catalysts, but it may result in a decrease in the catalytic performance of the NPs. Here, we show that well-defined colloidal silver, gold, and platinum NPs can be self-assembled in bacterial nanocellulose (BC) membranes, yielding BC-NP nanocomposites that are highly catalytically active using the reduction of 4-nitrophenol (4-NP) as a model reaction. The large effective surface area of BC enables the assembly of large quantities of NPs, resulting in materials with excellent catalytic performance. To address the mass transport limitations of reactants through the 3D nanofibrillar BC network, the membranes were dissociated using sonication to produce dispersed nanocellulose fibrils. This process dramatically reduced the time required for the adsorption of the NPs from days to minutes. Moreover, the catalytic performance of the nanofibril-supported NPs was drastically improved. A turnover frequency above 21,000 h(-1) was demonstrated, which is more than one order of magnitude higher than that for previously reported soft substrate-supported AuNP-based catalytic materials. The ease of fabrication, abundance, and low environmental footprint of the support material, along with reusability, stability, and unprecedented catalytic performance, make BC-NP nanocomposites a compelling option for green and sustainable catalysis.
|
|
| 8. |
|
|
| 9. |
- Zhang, Xin, et al.
(author)
-
A ratiometric fluorogenic nanoprobe for real-time quantitative monitoring of lysosomal pH
- 2021
-
In: Sensors and actuators. B, Chemical. - : Elsevier Science SA. - 0925-4005 .- 1873-3077. ; 345
-
Journal article (peer-reviewed)abstract
- Lysosomes are known as key players in cellular signalling and act as terminal degradation stations involved in a multitude of cellular processes. Being a highly influential physiological factor, pH is essential in the regulation of lysosome-mediated physiological and pathological processes. Aberrant pH fluctuations are highly related to lysosomal dysfunction that correlates to lysosomal storage diseases and neurodegenerative disorders. As such, real-time quantitative monitoring of lysosomal pH (pHL) is crucial for gaining insight into lysosomal dysfunction but challenging by the lack of effective lysosome-specific probes with high signal fidelity. Toward this end, we have proposed a lysosomal fluorogenic nanoprobe (TR-MP) for reliable ratiometric measuring of pHL. It is fabricated by rational manipulation of fluorescence resonance energy transfer (FRET) in a tailorable nanoplatform. The nanoprobe consists of biocompatible silica nanoparticles assembled with a pH-sensitive rhodamine derivative (RDM-TEOS) as an acceptor and aggregation-induced emission (AIE) fluorophore (TPE-OMe) as a donor to ensure high energy transfer efficiency. Further equipped with cell-penetrating facilitator and morpholine to enable effective cell-internalization and high lysosome affinity of TR-MP. Results show that TR-MP can quantitatively measure pH in a range of 3.0 - 7.0 and detect pHL fluctuations in live cells under various stimuli, as well as real-time monitor pHL during apoptosis.
|
|
| 10. |
- Zhang, Xin, et al.
(author)
-
Light-Up Lipid Droplets Dynamic Behaviors Using a Red-Emitting Fluorogenic Probe
- 2020
-
In: Analytical Chemistry. - : AMER CHEMICAL SOC. - 0003-2700 .- 1520-6882. ; 92:5, s. 3613-3619
-
Journal article (peer-reviewed)abstract
- Intracellular lipid metabolism occurs in lipid droplets (LDs), which is critical to the survival of cells. Imaging LDs is an intuitive way to understand their physiology in live cells. However, this is limited by the availability of specific probes that can properly visualize LDs in vivo. Here, an LDs-specific red-emitting probe is proposed to address this need, which is not merely with an ultrahigh signal-to-noise (S/N) ratio and a large Stokes shift (up to 214 nm) but also with superior resistance to photobleaching. The probe has been successfully applied to real-time tracking of intracellular LDs behaviors, including fusion, migration, and lipophagy processes. We deem that the proposed probe here offers a new possibility for deeper understanding of LDs-associated behaviors, elucidation of their roles and mechanisms in cellular metabolism, and determination of the transition between adaptive lipid storage and lipotoxicity as well.
|
|
| 11. |
- zhang, xin, 1990-, et al.
(author)
-
Single-wavelength-excited fluorogenic nanoprobe for accurate real-time ratiometric analysis of broad pH fluctuations in mitophagy
- 2022
-
In: Nano Reseach. - : Tsinghua University Press. - 1998-0124 .- 1998-0000. ; 15, s. 6515-6521
-
Journal article (peer-reviewed)abstract
- Mitophagy has a critical role in maintaining cellular homeostasis through acidic lysosomes engulfing excess or impaired mitochondria, thereby pH fluctuation is one of the most significant indicators for tracking mitophagy. Then such precise pH tracking demands the fluorogenic probe that has tailored contemporaneous features, including mitochondrial-specificity, excellent biocompatibility, wide pH-sensitive range of 8.0-4.0, and especially quantitative ability. However, available molecular probes cannot simultaneously meet all the requirements since it is extremely difficult to integrate multiple functionalities into a single molecule. To fully address this issue, we herein integrate two fluorogenic pH sensitive units, a mitochondria-specific block, cell-penetrating facilitator, and biocompatible segments into an elegant silica nano scaffold, which greatly ensures the applicability for real-time tracking of pH fluctuations in mitophagy. Most significantly, at a single wavelength excitation, the integrated pH-sensitive units have spectra-distinguishable fluorescence towards alkaline and acidic pH in a broad range that covers mitochondrial and lysosomal pH, thus enabling a ratiometric analysis of pH variations during the whole mitophagy. This work also provides constructive insights into the fabrication of advanced fluorescent nanoprobes for diverse biomedical applications.
|
|
| 12. |
- Zhang, Xin, et al.
(author)
-
Tailorable Membrane-Penetrating Nanoplatform for Highly Efficient Organelle-Specific Localization
- 2021
-
In: Small. - : Wiley-V C H Verlag GMBH. - 1613-6810 .- 1613-6829. ; 17:31
-
Journal article (peer-reviewed)abstract
- Given the breadth of currently arising opportunities and concerns associated with nanoparticles for biomedical imaging, various types of nanoparticles have been widely exploited, especially for cellular/subcellular level probing. However, most currently reported nanoparticles either have inefficient delivery into cells or lack specificity for intracellular destinations. The absence of well-defined nanoplatforms remains a critical challenge hindering practical nano-based bio-imaging. Herein, the authors elaborate on a tailorable membrane-penetrating nanoplatform as a carrier with encapsulated actives and decorated surfaces to tackle the above-mentioned issues. The tunable contents in such a versatile nanoplatform offer huge flexibility to reach the expected properties and functions. Aggregation-induced emission luminogen (AIEgen) is applied to achieve sought-after photophysical properties, specific targeting moieties are installed to give high affinity towards different desired organelles, and critical grafting of cell-penetrating cyclic disulfides (CPCDs) to promote cellular uptake efficiency without sacrificing the specificity. Hereafter, to validate its practicability, the tailored nano products are successfully applied to track the dynamic correlation between mitochondria and lysosomes during autophagy. The authors believe that the strategy and described materials can facilitate the development of functional nanomaterials for various life science applications.
|
|
