| 1. |
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| 2. |
- Ferreira, Jorge A., et al.
(författare)
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Spent sulphite liquor for cultivation of an edible Rhizopus sp.
- 2012
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Ingår i: BioResources. - : North Carolina State University: College of Natural Resources. - 1930-2126. ; 7:1, s. 173-188
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Tidskriftsartikel (refereegranskat)abstract
- Spent sulphite liquor, the major byproduct from the sulphite pulp production process, was diluted to 50% and used for production of an edible zygomycete Rhizopus sp. The focus was on production, yield, and composition of the fungal biomass composition. The fungus grew well at 20 to 40°C, but 32°C was found to be preferable compared to 20 and 40°C in terms of biomass production and yield (maximum of 0.16 g/g sugars), protein content (0.50-0.60 g/g), alkali-insoluble material (AIM) (ca 0.15 g/g), and glucosamine content (up to 0.30 g/g of AIM). During cultivation in a pilot airlift bioreactor, the yield increased as aeration was raised from 0.15 to 1.0 vvm, indicating a high demand for oxygen. After cultivation at 1.0 vvm for 84 h, high yield and production of biomass (up to 0.34 g/g sugars), protein (0.30-0.50 g/g), lipids (0.02-0.07 g/g), AIM (0.16-0.28 g/g), and glucosamine (0.22-0.32 g/g AIM) were obtained. The fungal biomass produced from spent sulphite liquor is presently being tested as a replacement for fishmeal in feed for fish aquaculture and seems to be a potential source of nutrients and for production of glucosamine.
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| 3. |
- Ferreira, Jorge A., et al.
(författare)
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Zygomycetes-based biorefinery: Present status and future prospects
- 2013
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Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 135, s. 523-532
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Tidskriftsartikel (refereegranskat)abstract
- Fungi of the phylum Zygomycetes fulfil all requirements for being utilized as core catalysts in biorefineries, and would be useful in creating new sustainable products. Apart from the extended use of Zygomycetes in preparing fermented foods, industrial metabolites such as lactic acid, fumaric acid, and ethanol are produced from a vast array of feedstocks with the aid of Zygomycetes. These fungi produce enzymes that facilitate their assimilation of various complex substrates, e.g., starch, cellulose, phytic acid, and proteins, which is relevant from an industrial point of view. The enzymes produced are capable of catalyzing various reactions involved in biodiesel production, preparation of corticosteroid drugs, etc. Biomass produced with the aid of Zygomycetes consists of proteins with superior amino acid composition, but also lipids and chitosan. The biomass is presently being tested for animal feed purposes, such as fish feed, as well as for lipid extraction and chitosan production. Complete or partial employment of Zygomycetes in biorefining procedures is consequently attractive, and is expected to be implemented within a near future.
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| 4. |
- Karimi, Keikhosro, et al.
(författare)
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Fed-batch cultivation of Mucor indicus in dilute-acid lignocellulosic hydrolyzate for ethanol production
- 2005
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Ingår i: BIOTECHNOLOGY LETTERS. - : Springer Science and Business Media LLC. - 0141-5492 .- 1573-6776. ; 27:18
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Tidskriftsartikel (refereegranskat)abstract
- Mucor indicus fermented dilute-acid lignocellulosic hydrolyzates to ethanol in fed-batch cultivation with complete hexose utilization and partial uptake of xylose. The fungus was tolerant to the inhibitors present in the hydrolyzates. It grew in media containing furfural (1 g/l), hydroxymethylfurfural (1 g/l), vanillin (1 g/l), or acetic acid (7 g/l), but did not germinate directly in the hydrolyzate. However, with fed-batch methodology, after initial growth of M. indicus in 500 ml enzymatic wheat hydrolyzate, lignocellulosic hydrolyzate was fermented with feeding rates 55 and 100 ml/h. The fungus consumed more than 46% of the initial xylose, while less than half of this xylose was excreted in the form of xylitol. The ethanol yield was 0.43 g/g total consumed sugar, and reached the maximum concentration of 19.6 g ethanol/l at the end of feeding phase. Filamentous growth, which is regarded as the main obstacle to large-scale cultivation of M. indicus, was avoided in the fed-batch experiments.
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| 5. |
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| 6. |
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| 7. |
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| 8. |
- Kondori, Nahid, 1967, et al.
(författare)
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Circulating beta (1-3) glucan and immunoglobulin G subclass antibodies to Candida albicans cell wall antigens in patients with systemic candidiasis.
- 2004
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Ingår i: Clinical and diagnostic laboratory immunology. - 1071-412X. ; 11:2, s. 344-50
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Tidskriftsartikel (refereegranskat)abstract
- Invasive candidiasis in patients who are immunocompromised or in intensive care units (ICUs) presents both diagnostic and therapeutic problems. We previously described antibodies that were directed against Candida albicans cell wall fragments (CW), periodate-treated CW (CW(IO4)), phosphopeptidomannan (PPM), and beta(1-3) glucan. In this study, circulating fungal antigens [mannan and beta(1-3) glucan] and immunoglobulin G (IgG) subclass antibodies to these cell wall antigens (anti-CW) were analyzed in patients with systemic candidiasis. Sera were collected from 14 patients on two or three consecutive occasions, starting on the day when candidiasis was culture proven. The sera were analyzed by enzyme-linked immunosorbent assay. The control groups consisted of lactating mothers (n = 9) (group I) who had breast milk that was positive for C. albicans and also had acute inflammation of the nipples, and age-matched blood donors (n = 10) (group II). Within the first 3 weeks of Candida infection all of the patients were positive for beta(1-3) glucan by the Gluspecy test, but no patients were positive for mannan in the less-sensitive Pastorex Candida test. The controls were negative for both beta(1-3) glucan (<20 pg/ml) and mannan (<2.5 ng/ml). IgG1 anti-CW and IgG2 anti-PPM antibodies were the most discriminatory antibodies. The ratio of IgG1 anti-CW to IgG2 anti-PPM was significantly lower in nonsurviving patients than in the other patients within the first week of candidiasis (P = 0.019). The IgG2 levels of anti-CW(IO4) and antiglucan antibodies correlated strongly (r = 0.681; P < 0.0001), and the absence of these antibodies was associated with increased levels of beta(1-3) glucan. Increased levels of IgG1 anti-CW or IgG2 anti-PPM antibodies (titer of > or = 3 logs) or of a combination of the two antibodies (log sum, > or = 5) showed 92% sensitivity, 100% specificity, and positive predictive values. In conclusion, beta(1-3) glucan and the two subclass antibodies appear to be early specific markers for the laboratory diagnosis of candidiasis. Furthermore, the kinetics of beta(1-3) glucan appearance in serum may assist in evaluating the therapeutic efficacy of antifungal treatments.
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| 9. |
- Lennartsson, Patrik, et al.
(författare)
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Growth tolerance of Zygomycetes Mucor indicus in orange peel hydrolysate without detoxification
- 2012
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Ingår i: Process Biochemistry. - : Elsevier Ltd. - 1359-5113 .- 1873-3298. ; 47:5, s. 836-842
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Tidskriftsartikel (refereegranskat)abstract
- The capability of two zygomycetes strains, Mucor indicus and an isolate from tempeh (Rhizopus sp.), to grow on orange peel hydrolysate and their tolerance to its antimicrobial activity, was investigated. Both fungi, in particular M. indicus, tolerated up to 2% d-limonene in semi-synthetic media during cultivation in shake flasks, under aerobic as well as anaerobic conditions. The tolerance of M. indicus was also tested in a bioreactor, giving rise to varying results in the presence of 2% limonene. Furthermore, both strains were capable of consuming galacturonic acid, the main monomer of pectin, under aerobic conditions when no other carbon source was present. The orange peel hydrolysate was based on 12% (dry w/v) orange peels, containing d-limonene at a concentration of 0.6% (v/v), which no other microorganism has been reported to be able to ferment. However, the hydrolysate was utilised by M. indicus under aerobic conditions, resulting in production of 410 and 400 mg ethanol/g hexoses and 57 and 75 mg fungal biomass/g sugars from cultivations in shake flasks and a bioreactor, respectively. Rhizopus sp., however, was slow to germinate aerobically, and neither of the zygomycetes was able to consistently germinate in orange peel hydrolysate, under anaerobic conditions. The zygomycetes strains used in the present study demonstrated a relatively high resistance to the antimicrobial compounds present in orange peel hydrolysate, and they were capable of producing ethanol and biomass in the presence of limonene, particularly when cultivated with air supply.
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| 10. |
- Lennartsson, Patrik R, 1983, et al.
(författare)
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Effects of different growth forms of Mucor indicus on cultivation on dilute-acid lignocellulosic hydrolyzate, inhibitor tolerance, and cell wall composition
- 2009
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Ingår i: Journal of Biotechnology. - : Elsevier BV. - 0168-1656 .- 1873-4863. ; 143:4, s. 255-261
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Tidskriftsartikel (refereegranskat)abstract
- The dimorphic fungus Mucor indicus was grown in different forms classified as purely filamentous, mostly filamentous, mostly yeast-like and purely yeast-like, and the relationship between morphology and metabolite production, inhibitor tolerance and the cell wall composition was investigated. Low concentrations of spores in the inoculum with subsequent aeration promoted filamentous growth, whereas higher spore concentrations and anaerobic conditions promoted yeast-like growth. Ethanol was the main metabolite with glycerol next under all conditions tested. The yields of ethanol from glucose were between 0.39 and 0.42 g g(-1) with productivities of 3.2-5.0 g l(-1) h(-1). The ethanol productivity of mostly filamentous cells was increased from 3.9 to 5.0 g l(-1) h(-1) by the presence of oxygen, whereas aeration of purely yeast-like cells showed no such effect. All growth forms were able to tolerate 4.6 g l(-1) furfural and 10 g l(-1) acetic acid and assimilate the sugars, although with different consumption rates. The cell wall content of the fungus measured as alkali insoluble materials (AIM) of the purely yeast-like cells was 26% of the biomass, compared to 8% of the pure filaments. However, the chitosan concentration of the filaments was 29% of the AIM, compared to 6% of the yeast-like cells.
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| 11. |
- Lennartsson, Patrik R, 1983, et al.
(författare)
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Ethanol production from lignocellulose by the dimorphic fungus Mucor indicus
- 2008
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Ingår i: World Bioenergy. Jönköping, Sweden, 27-29 May, 2008.
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Ethanol production from dilute-acid hydrolyzate by the dimorphic fungus Mucor indicus was investigated. A mixture of different forest wood chips dominated by spruce was hydrolyzed with 0.5 g/L sulfuric acid at 15 bar for 10 min, yielding different sugars including galactose, glucose, mannose, and xylose, but also different fermentation inhibitors such as acetic acid, furfural, hydroxymethyl furfural (HMF), and phenolic compounds. We induced different morphological growth of M. indicus from purely filamentous, mostly filamentous, mostly yeast-like to purely yeast-like. The different forms were then ysed to ferment the hydrolyzate. They tolerated the presence of the inhibitors under anaerobic batch cultivation well and the ethanol yield was 430-440 g/kg consumed sugars. The ethanol productivity depended on the morphology. Judging from these results, we conclude that M. indicus is useful for ethanol production from toxic substrates independent of its morphology.
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| 12. |
- Lennartsson, Patrik R, 1983, et al.
(författare)
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Rhizopus
- 2014
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Ingår i: Encyclopedia of Food Microbiology. - : Academic Press, Elsevier. - 9780123847300 ; , s. 284-290
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Bokkapitel (refereegranskat)
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| 13. |
- Lennartsson, Patrik R, 1983, et al.
(författare)
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Rhizopus
- 2014
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Ingår i: Encyclopedia of Food Microbiology. - 9780123847300 ; , s. 284-290
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 14. |
- Millati, Ria, 1972, et al.
(författare)
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Ethanol production from xylose and wood hydrolyzate by Mucor indicus at different aeration rates
- 2008
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Ingår i: BioResources. - : North Carolina State University. - 1930-2126. ; 3:4, s. 1020-1029
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Tidskriftsartikel (refereegranskat)abstract
- The fungus Mucor indicus is able to produce ethanol from xylose as well as dilute-acid lignocellulosic hydrolyzates. The fungus completely assimilated 10 g/L xylose as the sole carbon and energy source within 32 to 65 h at an aeration rate of 0.1 to 1.0 vvm. The highest ethanol yield was 0.16 g/g at 0.1 vvm. Xylitol was formed intermediately with a maximum yield of 0.22 g/g at 0.5 vvm., but disappeared towards the end of experiments. During cultivation in a mixture of xylose and glucose, the fungus did not assimilate xylose as long as glucose was present in the medium. The anaerobic cultivation of the fungus in the hydrolyzate containing 20% xylose and 80% hexoses resulted in no assimilation of xylose but complete consumption of the hexoses in less than 15 h. The ethanol yield was 0.44 g/g. However, the xylose in the hydrolyzate was consumed when the media were aerated at 0.067 to 0.333 vvm. The best ethanol yield was 0.44 g/g at 0.067 vvm. The results of this study suggest that M. indicus hydrolyzate can be first fermented anaerobically for hexose assimilation and subsequently continued under oxygen-limited conditions for xylose fermentation.
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| 15. |
- Millati, Ria, 1972, et al.
(författare)
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Performance of Rhizopus, Rhizomucor, and Mucor in ethanol production from glucose, xylose, and wood hydrolyzates
- 2005
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Ingår i: Enzyme and microbial technology. - : Elsevier BV. - 0141-0229 .- 1879-0909. ; 36:2-3, s. 294-300
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Tidskriftsartikel (refereegranskat)abstract
- In searching for ethanol producing microorganisms also capable of fermenting pentoses, nine zygomycetes strains including three strains of Rhizopus oryzae, Mucor corticolous, M. hiemalis, M. indicus, Rhizomucor pusillus, R. miehei, and zygomycete IT were examined. Each strain was cultivated on glucose, xylose or dilute-acid hydrolyzate (DAH) as carbon sources, and the production of ethanol, lactic acid, glycerol, xylitol, and succinic acid were investigated. Great similarities but also conspicuous differences were seen between the species, to some extent linked to the genera. All strains were capable of growing on glucose or xylose as single carbon source. With the exception of the two Rhizomucor strains, all produced ethanol. All the strains produced glycerol as by-product, while Rhizopus and Rhizomucor but not Mucor produced lactic acid in significant amounts. All Mucor and Rhizopus strains and one strain of Rhizomucor produced xylitol in the xylose medium, but no xylitol was detected after growth on DAH. All Mucor and two R. oryzae strains were capable of growing on DAH. Two Mucor species, M. hiemalis and M. indicus showed greater ethanol production than the other strains. The ethanol yields by M. hiemalis on glucose, xylose, and DAH were 0.39, 0.18, and 0.44 g/g, respectively, whereas the corresponding results for M. indicus were 0.39, 0.22, and 0.44 g/g. The strains also rapidly consumed hydroxymethyl furfural present in DAH.
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| 16. |
- Sues, Anna, et al.
(författare)
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Ethanol production from hexoses, pentoses, and dilute-acid hydrolyzate by Mucor indicus
- 2005
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Ingår i: FEMS Yeast Research. - : Oxford University Press (OUP). - 1567-1356 .- 1567-1364. ; 5:6-7, s. 669-676
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Tidskriftsartikel (refereegranskat)abstract
- Consumption of hexoses and pentoses and production of ethanol by Mucor indicus were investigated in both synthetic media and dilute-acid hydrolyzates. The fungus was able to grow in a poor medium containing only carbon, nitrogen, phosphate, potassium, and magnesium sources. However, the cultivation took more than a week and the ethanol yield was only 0.2 g g -1 . Enrichment of the medium by addition of trace metals, particularly zinc and yeast extract, improved the growth rate and yield, such that the cultivation was completed in less than 24 h and the ethanol and biomass yields were increased to 0.40 and 0.20 g g -1 , respectively. The fungus was able to assimilate glucose, galactose, mannose, and xylose, and produced ethanol with yields of 0.40, 0.34, 0.39, and 0.18 g g -1 , respectively. However, arabinose was poorly consumed and no formation of ethanol was detected. Glycerol was the major by-product in the cultivation on the hexoses, while formation of glycerol and xylitol were detected in the cultivation of the fungus on xylose. The fungus was able to take up the sugars present in dilute-acid hydrolyzate as well as the inhibitors, acetic acid, furfural, and hydroxymethyl furfural. M. indicus was able to grow under anaerobic conditions when glucose was the sole carbon source, but not on xylose or the hydrolyzate. The yield of ethanol in anaerobic cultivation on glucose was 0.46 g g -1 . © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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| 17. |
- Zamani, Akram, et al.
(författare)
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Determination of glucosamine and N-acetyl glucosamine in fungal cell walls
- 2008
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Ingår i: Journal of Agricultural and Food Chemistry. - : American Chemical Society. - 0021-8561 .- 1520-5118. ; 56:18, s. 8314-8318
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Tidskriftsartikel (refereegranskat)abstract
- A new method was developed to determine glucosamine (GlcN) and N-acetyl glucosamine (GlcNAc) in materials containing chitin and chitosan, such as fungal cell walls. It is based on two steps of hydrolysis with (i) concentrated sulfuric acid at low temperature and (ii) dilute sulfuric acid at high temperature, followed by one-step degradation with nitrous acid. In this process, chitin and chitosan are converted into anhydromannose and acetic acid. Anhydromannose represents the sum of GlcN and GlcNAc, whereas acetic acid is a marker for GlcNAc only. The method showed recovery of 90.1% of chitin and 85.7-92.4% of chitosan from commercial preparations. Furthermore, alkali insoluble material (AIM) from biomass of three strains of zygomycetes, Rhizopus oryzae, Mucor indicus, and Rhizomucor pusillus, was analyzed by this method. The glucosamine contents of AIM from R. oryzae and M. indicus were almost constant (41.7 +/- 2.2% and 42.0 +/- 1.7%, respectively), while in R. pusillus, it decreased from 40.0 to 30.0% during cultivation from 1 to 6 days. The GlcNAc content of AIM from R. oryzae and R. pusillus increased from 24.9 to 31.0% and from 36.3 to 50.8%, respectively, in 6 days, while it remained almost constant during the cultivation of M. indicus (23.5 +/- 0.8%).
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| 18. |
- Zamani Forooshani, Akram, 1981, et al.
(författare)
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Extraction and precipitation of chitosan from cell wall of zygomycetes fungi by dilute sulfuric acid
- 2007
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Ingår i: Biomacromolecules. - : American Chemical Society (ACS). - 1525-7797 .- 1526-4602. ; 8:12, s. 3786-3790
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Tidskriftsartikel (refereegranskat)abstract
- A new method was developed in this work for extraction of chitosan from the zygomycetes cell wall. It is based on the temperature-dependent solubility of chitosan in dilute sulfuric acid. Chitin is soluble in neither cold nor hot dilute sulfuric acid. Similarly chitosan is not soluble at room temperature but is dissolved in 1% H2SO4 at 121 degrees C within 20 min. The new method was developed to measure the chitosan content of the biomass and cell wall. The procedures were investigated by measuring phosphate, protein, ash, glucuronic acid, and degree of acetylation. The cell wall derivatives of fungus Rhizomucor pusillus were then examined by this new method. The results indicated 8% of the biomass as chitosan. After treatment with NaOH, the alkali-insoluble material (AIM) contained 45.3% chitosan. Treatment of AIM with acetic acid resulted in 16.5% acetic-acid-soluble material (AGSM) and 79.0% alkali- and acid-insoluble material (AAIM). AGSM is usually cited as pure chitosan, but the new method shows major impurities by, for example, phosphate. Furthermore, AAIM is usually considered to be the chitosan-free fraction, whereas the new method shows more than 76% of the chitosan present in AIM is found in AAIM. It might indicate the inability of acetic acid to separate chitosan from the cell wall.
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| 19. |
- Zamani Forooshani, Akram, 1981, et al.
(författare)
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Temperature Shifts for Extraction and Purification of Zygomycetes Chitosan with Dilute Sulfuric Acid
- 2010
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Ingår i: International Journal of Molecular Sciences. - : MDPI AG. - 1661-6596 .- 1422-0067. ; 11:8, s. 2976-2987
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Tidskriftsartikel (refereegranskat)abstract
- The temperature-dependent hydrolysis and solubility of chitosan in sulfuric acidsolutions offer the possibility for chitosan extraction from zygomycetes mycelia andseparation from other cellular ingredients with high purity and high recovery. In this study,Rhizomucor pusillus biomass was initially extracted with 0.5 M NaOH at 120 °C for20 min, leaving an alkali insoluble material (AIM) rich in chitosan. Then, the AIM wassubjected to two steps treatment with 72 mM sulfuric acid at (i) room temperature for10 min followed by (ii) 120 °C for 45 min. During the first step, phosphate of the AIM wasreleased into the acid solution and separated from the chitosan-rich residue bycentrifugation. In the second step, the residual AIM was re-suspended in fresh 72 mMsulfuric acid, heated at 120 °C and hot filtered, whereby chitosan was extracted andseparated from the hot alkali and acid insoluble material (HAAIM). The chitosan wasrecovered from the acid solution by precipitation at lowered temperature and raised pH to8-10. The treatment resulted in 0.34 g chitosan and 0.16 g HAAIM from each gram AIM.At the start, the AIM contained at least 17% phosphate, whereas after the purification, thecorresponding phosphate content of the obtained chitosan was just 1%. The purity of thischitosan was higher than 83%. The AIM subjected directly to the treatment with hotsulfuric acid (at 120 °C for 45 min) resulted in a chitosan with a phosphate impurity of 18.5%.
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