SwePub - sökning: WFRF:(Feng Wenxun)

Numrering	Referens	Omslagsbild	Hitta
1.	Shen, Jie, et al. (författare) Real-time monitoring of lipid droplets growth via the fusion with fluorescent dye-labeled adiposomes 2020 Ingår i: Dyes and pigments. - : ELSEVIER SCI LTD. - 0143-7208 .- 1873-3743. ; 182 Tidskriftsartikel (refereegranskat)abstract Investigating lipid droplets (LDs) behaviours is essential to deeply understand the physiology of LDs, such as their growths, movements, fusion/division, and autophagy. Among these behaviours, the growth of LDs is one of the most difficult to track due to the very subtle morphology evolution in a short time window. The major obstacle is that conventional LDs-specific dyes with low photostability cannot indicate the LDs size change. To address this issue, we synthesize a hydrophobic and photostable fluorescent dye (TPA-AD) and load it into the neutral lipid micelles (as artificial adiposomes). The highly hydrophobic TPA-AD enables the specific accumulation into intracellular LDs and the ready loading artificial adiposomes. When the intracellular LDs take TPA-AD-labeled adiposomes, by fusion, the sizes of LDs gradually grow, and LDs are simultaneously lighted up by the fluorescence of TPA-AD. Importantly, the high photostability of TPA-AD ensures the enhanced fluorescence signals. The finding here will further strengthen the understanding of LDs dynamics and fat metabolism.

Shen, Jie, et al. (författare)
Real-time monitoring of lipid droplets growth via the fusion with fluorescent dye-labeled adiposomes
2020
Ingår i: Dyes and pigments. - : ELSEVIER SCI LTD. - 0143-7208 .- 1873-3743. ; 182
Tidskriftsartikel (refereegranskat)abstract
- Investigating lipid droplets (LDs) behaviours is essential to deeply understand the physiology of LDs, such as their growths, movements, fusion/division, and autophagy. Among these behaviours, the growth of LDs is one of the most difficult to track due to the very subtle morphology evolution in a short time window. The major obstacle is that conventional LDs-specific dyes with low photostability cannot indicate the LDs size change. To address this issue, we synthesize a hydrophobic and photostable fluorescent dye (TPA-AD) and load it into the neutral lipid micelles (as artificial adiposomes). The highly hydrophobic TPA-AD enables the specific accumulation into intracellular LDs and the ready loading artificial adiposomes. When the intracellular LDs take TPA-AD-labeled adiposomes, by fusion, the sizes of LDs gradually grow, and LDs are simultaneously lighted up by the fluorescence of TPA-AD. Importantly, the high photostability of TPA-AD ensures the enhanced fluorescence signals. The finding here will further strengthen the understanding of LDs dynamics and fat metabolism.

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