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- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2. |
- Blennow Nordström, Erik, et al.
(författare)
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Cognition as independent predictor of functional outcome after cardiac arrest
- 2024
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Ingår i: ; , s. 22-22
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Konferensbidrag (refereegranskat)abstract
- BackgroundCognitive function is often impaired for cardiac arrest (CA) survivors as an effect of hypoxic-ischemic brain injury. An important factor in CA recovery is global functional outcome, commonly assessed with the modified Rankin Scale (mRS). Little is known about the role of cognition as a potential predictor of outcome following CA.ObjectiveTo investigate cognitive function at hospital discharge as a predictor of global functional outcome at 1-month post-discharge. Our hypothesis was that cognitive function would be a significant independent predictor of functional outcome after adjusting for sociodemographic and medical factors.MethodAdult CA survivors were recruited at Columbia University Medical Center, New York, in the ongoing Psychological Predictors of Recovery after Acute Cardiac Events (PACE) Study. Cognition was measured with the Telephone Interview for Cognitive Status Modified (TICS-M) at hospital discharge, and mRS was the functional outcome scale 1 month later. We analyzed our data with stepwise logistic regressions: 1) unadjusted; 2) adjusted for age, sex, educational attainment, race/ethnicity, time from CA to return of spontaneous circulation; 3) additionally adjusted for depressive symptoms (Patient Health Questionnaire-8) atdischarge.ResultsIn our cohort of 112 participants (median age=56, 62% male) with completed TICS-M at discharge, 71% scored <36 indicating cognitive impairment, and 61% had mRS scores >3 equivalent to poor functional outcome at 1-month. Higher TICS-M scores at discharge independently predicted lower risk of poor 1-month mRS scores in all regression models, OR=0.84 (95% CI [0.74, 0.94]), p <.01 (model 3). Women had a greater risk of poor functional outcome. Hispanic participants had greater chances of good functional outcome.ConclusionIndicated cognitive impairment was a significant independent predictor of poor functional outcome after CA beyond the effects of demographics and CA downtime. This emphasizes the importance of targeting cognition in rehabilitation interventions, but also to identify other risk factors that could impede post-CA recovery.
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3. |
- Blennow Nordström, Erik, et al.
(författare)
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Prospective evaluation of the relationship between cognition and recovery outcomes after cardiac arrest
- 2024
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Ingår i: Resuscitation. - 0300-9572. ; 202
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: Cognitive function is often impaired for cardiac arrest (CA) survivors due to hypoxic-ischemic brain injury. Whether cognitive impairment at hospital discharge is associated with recovery defined as functional status and fatigue measured at 1-month post-discharge is not known.Methods: Consecutive CA patients admitted at an academic center (May 14, 2021–June 23, 2023) were assessed for cognitive impairment (modified Telephone Interview for Cognitive Status, TICS-m < 33) and depressive symptoms (8-item Patient Health Questionnaire) at hospital discharge. Poor functional status (primary outcome; modified Rankin Scale, mRS > 3) and fatigue severity (patient-reported outcome; Modified Fatigue Impact Scale) were assessed 1-month post-discharge. Hierarchical regressions tested associations of cognitive function with outcomes.Results: Of 112 participants (mean age 54.4 ± 14.8; 38% female; 43% White race, 20% Black race, 29% Hispanic ethnicity) completing discharge TICS-m, 63 (56%) had indicated cognitive impairment, and 68 (61%) had poor 1-month functional outcome. Worse discharge cognitive function was independently associated with a higher risk of poor 1-month functional outcome (OR = 0.88, 95% CI [0.79, 0.98], p = 0.02) after adjusting for age, education, sex, race, ethnicity, length of hospital stay, comorbidities, and depressive symptoms. Fatigue severity lacked significant associations with cognitive function, but was associated with depressive symptoms (B = 1.03 [0.00, 2.05], p = 0.04).Conclusion: Cognitive function at discharge after CA was significantly and independently associated with functional outcome 1 month after hospital discharge. Psychological distress contributed to fatigue severity. This highlights the need for screening and addressing cognitive and emotional problems pre-hospital discharge.
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