| 1. |
- Alström, Sadhna, et al.
(författare)
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Straw microorganisms with combined cellulolytic and chitinolytic activities drive decomposition of rice straw and antagonism against Rhizoctonia solani causing rice sheath blight
- 2023
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Ingår i: International Journal of Agricultural Technology. - 2630-0192. ; 19, s. 833-848
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Tidskriftsartikel (refereegranskat)abstract
- Decomposition of cellulose and chitin substrates plays an important role in ecosystem functions. The relationships between the microbial diversity and the ecosystem functions of decomposition and antagonistic processes were examined using rice straw. A microcosm was designed for investigating complexity in microbial interactions of 16 different microorganisms belonging to 6 different functional groups and were designated as generalists or specialists depending on the number of hydrolases produced. Multiple regression analysis showed that increasing number of isolates, the presence of three fungal and one bacterial isolate and two fungal isolates degraded significantly to the decomposition process of rice straw. Straw was decomposed by one fungus and one bacterium, three fungal and two bacterial isolates and the 16-isolates mixture were significantly inhibited growth of the phytopathogenic Rhizoctonia solani causing rice sheath blight on the decomposed straw.The growth of R. solani was reduced significantly by 62% - 93% compared to the non-inoculated controls. There was a negative correlation between rice straw weight loss and the growth of R. solani. It is suggested that the specific isolates may play an important role in ecosystem functions of decomposition and antagonistic processes.
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| 2. |
- Askmyr, Maria, et al.
(författare)
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Modeling chronic myeloid leukemia in immunodeficient mice reveals expansion of aberrant mast cells and accumulation of pre-B cells.
- 2014
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Ingår i: Blood Cancer Journal. - : Springer Science and Business Media LLC. - 2044-5385. ; 4
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Tidskriftsartikel (refereegranskat)abstract
- Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm that, if not treated, will progress into blast crisis (BC) of either myeloid or B lymphoid phenotype. The BCR-ABL1 fusion gene, encoding a constitutively active tyrosine kinase, is thought to be sufficient to cause chronic phase (CP) CML, whereas additional genetic lesions are needed for progression into CML BC. To generate a humanized CML model, we retrovirally expressed BCR-ABL1 in the cord blood CD34(+) cells and transplanted these into NOD-SCID (non-obese diabetic/severe-combined immunodeficient) interleukin-2-receptor γ-deficient mice. In primary mice, BCR-ABL1 expression induced an inflammatory-like state in the bone marrow and spleen, and mast cells were the only myeloid lineage specifically expanded by BCR-ABL1. Upon secondary transplantation, the pronounced inflammatory phenotype was lost and mainly human mast cells and macrophages were found in the bone marrow. Moreover, a striking block at the pre-B-cell stage was observed in primary mice, resulting in an accumulation of pre-B cells. A similar block in B-cell differentiation could be confirmed in primary cells from CML patients. Hence, this humanized mouse model of CML reveals previously unexplored features of CP CML and should be useful for further studies to understand the disease pathogenesis of CML.
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| 3. |
- Askmyr, Maria, et al.
(författare)
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Transgenic expression of human cytokines in immunodeficient mice does not facilitate myeloid expansion of BCR-ABL1 transduced human cord blood cells
- 2017
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Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 12:10
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Tidskriftsartikel (refereegranskat)abstract
- Several attempts have been made to model chronic myeloid leukemia (CML) in a xenograft setting but expansion of human myeloid cells in immunodeficient mice has proven difficult to achieve. Lack of cross-reacting cytokines in the microenvironment of the mice has been proposed as a potential reason. In this study we have used NOD/SCID IL2–receptor gamma deficient mice expressing human SCF, IL-3 and GM-CSF (NSGS mice), that should be superior in supporting human, and particularly, myeloid cell engraftment, to expand BCR-ABL1 expressing human cells in order to model CML. NSGS mice transplanted with BCR-ABL1 expressing cells became anemic and had to be sacrificed due to illness, however, this was not accompanied by an expansion of human myeloid cells but rather we observed a massive expansion of human T-cells and macrophages/histiocytes. Importantly, control human cells without BCR-ABL1 expression elicited a similar reaction, although with a slight delay of disease induction, suggesting that while BCR-ABL1 contributes to the inflammatory reaction, the presence of normal human hematopoietic cells is detrimental for NSGS mice.
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| 4. |
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| 5. |
- Bila, Joao, et al.
(författare)
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First report of 'Candidatus Phytoplasma palmicola' detection in the planthopper Diostrombus mkurangai in Mozambique
- 2017
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Ingår i: Bulletin of insectology. - 1721-8861. ; 70, s. 45-48
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Tidskriftsartikel (refereegranskat)abstract
- Knowledge of putative insect species vectors of the coconut lethal yellowing disease (CLYD) in Mozambique is crucial to develop an effective disease management plan. Hemiptera specimens from the families Derbidae and Pentatomidae were collected in the Inhambane and Zambezia provinces of the coastal region of Mozambique in 2014, covering the two main growing seasons. Sequence analyses of the 16S rRNA gene were used for phytoplasma clustering. Polymerase chain reaction (PCR) amplification was performed employing three different primer sets specific for phytoplasma. BLAST sequence comparison and phylogenetic analysis of the 16S rDNA PCR products revealed that collected specimens of Derbidae Diostrombus mkurangai Wilson were carrying the CLYD phytoplasma. Virtual RFLP analyses of the obtained sequences confirmed this assigning the detected phytoplasmas to the 16SrXX-A subgroup, confirming that they are 'Candidatus Phytoplasma palmicola'-related strains. This is the first detection of a 'Candidatus Phytoplasma palmicola'-related strain in D. mkurangai.
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| 6. |
- Bila, Joao, et al.
(författare)
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High diversity, expanding populations and purifying selection in phytoplasmas causing coconut lethal yellowing in Mozambique
- 2015
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Ingår i: Plant Pathology. - : Wiley. - 0032-0862 .- 1365-3059. ; 64, s. 597-604
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Tidskriftsartikel (refereegranskat)abstract
- In this study, the putative phytoplasma species causing coconut lethal yellowing disease in Mozambique and Tanzania were characterized. The 16S rRNA and secA genes were sequenced. Phylogenetic analysis revealed that Mozambican coconut phytoplasmas belong to three different types: Candidatus Phytoplasma palmicola' 16SrXXII-A, a second strain that was previously isolated in Tanzania and Kenya (16SrIV-C), and a third strain that was different from all known lethal yellowing phytoplasma species. The third strain potentially represents a novel species and is closely related to pine phytoplasma. Co-infection with Ca. Phytoplasma pini'-related and Ca. Phytoplasma palmicola' 16SrXXII-A strains was observed. Furthermore, sequence variation in Ca. Phytoplasma palmicola' at the population level was consistent with purifying selection and population expansion.
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| 7. |
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| 8. |
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| 9. |
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| 10. |
- Blandón-Díaz, Jorge Ulises, et al.
(författare)
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Phenotypic Variation Within a Clonal Lineage of Phytophthora infestans Infecting both Tomato and Potato in Nicaragua
- 2012
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Ingår i: Phytopathology. - 0031-949X .- 1943-7684. ; 102, s. 323-330
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Tidskriftsartikel (refereegranskat)abstract
- Late blight caused by Phytophthora infestans (Mont.) de Bary is a constraint to both potato and tomato crops in Nicaragua. The hypothesis that the Nicaraguan population of P. infestans is genotypically and phenotypically diverse and potentially subdivided based on host association was tested. A collection of isolates was analyzed using genotypic markers (microsatellites and mitochondrial DNA haplotype) and phenotypic markers (mating type, virulence, and fungicide sensitivity). The genotypic analysis revealed no polymorphism in 121 of 132 isolates of P. infestans tested. Only the Ia haplotype and the A2 mating type were detected. Most of the tested isolates were resistant to metalaxyl. The virulence testing showed variation among isolates of P. infestans. No evidence was found of population differentiation among potato and tomato isolates of P. infestans based on the genotypic and phenotypic analysis. We conclude that the Nicaraguan population of P. infestans consists of a single clonal lineage (NI-1) which belongs to the A2 mating type and the Ia mitochondrial DNA haplotype. Moreover, based on the markers used, this population of P. infestans does not resemble the population in countries from which potato seed is imported to Nicaragua or the population in neighboring countries. The data presented here indicate that the NI-1 clonal lineage is the primary pathogen on both potato and tomato, and its success on both host species is unique in a South American context.
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| 11. |
- Brandström Durling, Mikael, et al.
(författare)
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The fungus that came in from the cold: dry rot's pre-adapted ability to invade buildings
- 2018
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Ingår i: ISME Journal. - : Springer Science and Business Media LLC. - 1751-7362 .- 1751-7370. ; 12, s. 791-801
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Tidskriftsartikel (refereegranskat)abstract
- Many organisms benefit from being pre-adapted to niches shaped by human activity, and have successfully invaded man-made habitats. One such species is the dry rot fungus Serpula lacrymans, which has a wide distribution in buildings in temperate and boreal regions, where it decomposes coniferous construction wood. Comparative genomic analyses and growth experiments using this species and its wild relatives revealed that S. lacrymans evolved a very effective brown rot decay compared to its wild relatives, enabling an extremely rapid decay in buildings under suitable conditions. Adaptations in intracellular transport machineries promoting hyphal growth, and nutrient and water transport may explain why it is has become a successful invader of timber in houses. Further, we demonstrate that S. lacrymans has poor combative ability in our experimental setup, compared to other brown rot fungi. In sheltered indoor conditions, the dry rot fungus may have limited encounters with other wood decay fungi compared to its wild relatives. Overall, our analyses indicate that the dry rot fungus is an ecological specialist with poor combative ability against other fungi.
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| 12. |
- Do Thi, Xuan, et al.
(författare)
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Different crop rotation systems as drivers of change in soil bacterial community structure and yield of rice, Oryza sativa
- 2011
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Ingår i: Biology and Fertility of Soils. - : Springer Science and Business Media LLC. - 0178-2762 .- 1432-0789. ; 48, s. 1-9
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Tidskriftsartikel (refereegranskat)abstract
- Intensive cropping, especially of rice, is considered to contribute to negative effects not only on soil chemical and biological properties but also on long-term grain yield. Appropriate crop rotation is often practiced as an alternative strategy to overcome the negative side effects of intensive cropping. Although soil microbial diversity and community structure have been shown to respond differently to altered agricultural management practices, little is known about possible links between crop rotation and grain yield on bacterial communities in rice paddy soil. In this study, we investigated the impact of specific rotational crops and compared it with intensive rice cultivation. The main crop rice (Oryza sativa) was rotated with maize (Zea mays) and mungbean (Phaseolus aureus) in different combinations in a system cultivating three crops per year. Soil bacterial communities were studied in two different cropping periods using pyrosequencing of the variable V4 region of the 16s rRNA. Our results showed that rotation with alternative crops increased rice yield by 24- 46% depending on rotation structure and that bacterial community structure was altered in the presence of mungbean and/or maize compared to that in rice monoculture. In the crop rotation systems, composition, abundance, and diversity of soil bacterial communities were significantly different and higher than those in rice monoculture. Our results show that effects of crop rotation relate to changes in soil bacterial community structure suggesting that appropriate crop rotations provide a feasible practice to maintain the equilibrium in soil microbial environment for sustainable rice cultivation
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| 13. |
- Finlay, Roger, et al.
(författare)
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Complete genome sequence of Serratia plymuthica strain AS12
- 2012
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Ingår i: Standards in Genomic Sciences. - : Springer Science and Business Media LLC. - 1944-3277. ; 6, s. 165-173
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Tidskriftsartikel (refereegranskat)abstract
- A plant-associated member of the family Enterobacteriaceae, Serratia plymuthica strain AS12 was isolated from rapeseed roots. It is of scientific interest because it promotes plant growth and inhibits plant pathogens. The genome of S. plymuthica AS12 comprises a 5,443,009 bp long circular chromosome, which consists of 4,952 protein-coding genes, 87 tRNA genes and 7 rRNA operons. This genome was sequenced within the 2010 DOE-JGI Community Sequencing Program (CSP2010) as part of the project entitled "Genomics of four rapeseed plant growth promoting bacteria with antagonistic effect on plant pathogens".
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| 14. |
- Gkarmiri, Konstantia, et al.
(författare)
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Identifying the Active Microbiome Associated with Roots and Rhizosphere Soil of Oilseed Rape
- 2017
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Ingår i: Applied and Environmental Microbiology. - : American Society for Microbiology. - 0099-2240 .- 1098-5336. ; 83:22
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Tidskriftsartikel (refereegranskat)abstract
- RNA stable isotope probing and high-throughput sequencing were used to characterize the active microbiomes of bacteria and fungi colonizing the roots and rhizosphere soil of oilseed rape to identify taxa assimilating plant-derived carbon following (13)CO2 labeling. Root- and rhizosphere soil-associated communities of both bacteria and fungi differed from each other, and there were highly significant differences between their DNA- and RNA-based community profiles. Verrucomicrobia, Proteobacteria, Planctomycetes, Acidobacteria, Gemmatimonadetes, Actinobacteria, and Chloroflexi were the most active bacterial phyla in the rhizosphere soil. Bacteroidetes were more active in roots. The most abundant bacterial genera were well represented in both the (13)C- and (12)C-RNA fractions, while the fungal taxa were more differentiated. Streptomyces, Rhizobium, and Flavobacterium were dominant in roots, whereas Rhodoplanes and Sphingomonas (Kaistobacter) were dominant in rhizosphere soil. "Candidatus Nitrososphaera" was enriched in (13)C in rhizosphere soil. Olpidium and Dendryphion were abundant in the (12)C-RNA fraction of roots; Clonostachys was abundant in both roots and rhizosphere soil and heavily (13)C enriched. Cryptococcus was dominant in rhizosphere soil and less abundant, but was (13)C enriched in roots. The patterns of colonization and C acquisition revealed in this study assist in identifying microbial taxa that may be superior competitors for plant-derived carbon in the rhizosphere of Brassica napusIMPORTANCE This microbiome study characterizes the active bacteria and fungi colonizing the roots and rhizosphere soil of Brassica napus using high-throughput sequencing and RNA-stable isotope probing. It identifies taxa assimilating plant-derived carbon following (13)CO2 labeling and compares these with other less active groups not incorporating a plant assimilate. Brassica napus is an economically and globally important oilseed crop, cultivated for edible oil, biofuel production, and phytoextraction of heavy metals; however, it is susceptible to several diseases. The identification of the fungal and bacterial species successfully competing for plant-derived carbon, enabling them to colonize the roots and rhizosphere soil of this plant, should enable the identification of microorganisms that can be evaluated in more detailed functional studies and ultimately be used to improve plant health and productivity in sustainable agriculture.
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| 15. |
- Gkarmiri, Konstantia, et al.
(författare)
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Transcriptomic changes in the plant pathogenic fungus Rhizoctonia solani AG-3 in response to the antagonistic bacteria Serratia proteamaculans and Serratia plymuthica
- 2015
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Ingår i: BMC Genomics. - : Springer Science and Business Media LLC. - 1471-2164. ; 16
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Tidskriftsartikel (refereegranskat)abstract
- Background: Improved understanding of bacterial-fungal interactions in the rhizosphere should assist in the successful application of bacteria as biological control agents against fungal pathogens of plants, providing alternatives to chemicals in sustainable agriculture. Rhizoctonia solani is an important soil-associated fungal pathogen and its chemical treatment is not feasible or economic. The genomes of the plant-associated bacteria Serratia proteamaculans S4 and Serratia plymuthica AS13 have been sequenced, revealing genetic traits that may explain their diverse plant growth promoting activities and antagonistic interactions with R. solani. To understand the functional response of this pathogen to different bacteria and to elucidate whether the molecular mechanisms that the fungus exploits involve general stress or more specific responses, we performed a global transcriptome profiling of R. solani Rhs1AP anastomosis group 3 (AG-3) during interaction with the S4 and AS13 species of Serratia using RNA-seq.Results: Approximately 104,504 million clean 75-100 bp paired-end reads were obtained from three libraries, each in triplicate (AG3-Control, AG3-S4 and AG3-AS13). Transcriptome analysis revealed that approximately 10 % of the fungal transcriptome was differentially expressed during challenge with Serratia. The numbers of S4- and AS13-specific differentially expressed genes (DEG) were 866 and 292 respectively, while there were 1035 common DEGs in the two treatment groups. Four hundred and sixty and 242 genes respectively had values of log(2) fold-change > 3 and for further analyses this cut-off value was used. Functional classification of DEGs based on Gene Ontology enrichment analysis and on KEGG pathway annotations revealed a general shift in fungal gene expression in which genes related to xenobiotic degradation, toxin and antioxidant production, energy, carbohydrate and lipid metabolism and hyphal rearrangements were subjected to transcriptional regulation.Conclusions: This RNA-seq profiling generated a novel dataset describing the functional response of the phytopathogen R. solani AG3 to the plant-associated Serratia bacteria S4 and AS13. Most genes were regulated in the same way in the presence of both bacterial isolates, but there were also some strain-specific responses. The findings in this study will be beneficial for further research on biological control and in depth exploration of bacterial-fungal interactions in the rhizosphere.
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| 16. |
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| 17. |
- Högberg, Nils
(författare)
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Contrasting demographic histories revealed in two invasive populations of the dry rot fungus Serpula lacrymans
- 2021
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Ingår i: Molecular Ecology. - : Wiley. - 0962-1083 .- 1365-294X. ; 30, s. 2772-2789
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Tidskriftsartikel (refereegranskat)abstract
- Globalization and international trade have impacted organisms around the world leading to a considerable number of species establishing in new geographic areas. Many organisms have taken advantage of human-made environments, including buildings. One such species is the dry rot fungus Serpula lacrymans, which is the most aggressive wood-decay fungus in indoor environments in temperate regions. Using population genomic analyses of 36 full genome sequenced isolates, we demonstrated that European and Japanese isolates are highly divergent and the populations split 3000-19,000 generations ago, probably predating human influence. Approximately 250 generations ago, the European population went through a tight bottleneck, probably corresponding to the fungus colonization of the built environment in Europe. The demographic history of these populations, probably lead to low adaptive potential. Only two loci under selection were identified using a F-st outlier approach, and selective sweep analyses identified three loci with extended haplotype homozygosity. The selective sweep analyses found signals in genes possibly related to decay of various substrates in Japan and in genes involved DNA replication and protein modification in Europe. Our results suggest that the dry rot fungus independently established in indoor environments in Europe and Japan and that invasive species can potentially establish large populations in new habitats based on a few colonizing individuals.
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| 18. |
- Högberg, Nils
(författare)
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Effect of some soil chemical properties on the occurrence of arbuscular mycorrhizal fungi in soils low pH growing rice plant in the Mekong Delta, Vietnam
- 2023
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Ingår i: International Journal of Agricultural Technology. - 2630-0192. ; 19, s. 1407-1420
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Tidskriftsartikel (refereegranskat)abstract
- The results showed that about 7 – 64% of the rice roots were infected by AMF community. The infection structure included 3 main types of hyphae, arbuscules and vesicules. The density of AMF spores presented in the soil samples ranged from 500 to 1635 spores/100g of dry soil. The percentage of the AMF colonization had a positive correlation with soil pH value (r=0.72*), a negative correlation with electric conductivity EC (r=-0.87*), with organic matter content (r=-0.77*), with total phosphorouscontent (r=-0.71*) and positive correlation with the number of AMF spores (r=0.71*). The research illustrated the symbiosis system between AMF and rice roots and the correlation between the occurrence of AMF and soil pH, EC, organic matter content as well as the total phosphorous content in paddy fields under the soil low pH.
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| 19. |
- Högberg, Nils
(författare)
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Evolutionary origin, worldwide dispersal, and population genetics of the dry rot fungus Serpula lacrymans
- 2012
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Ingår i: Fungal Biology Reviews. - : Elsevier BV. - 1749-4613 .- 1878-0253. ; 26, s. 84-93
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Forskningsöversikt (refereegranskat)abstract
- Recent research on the evolution, phylogeography and population genetics of the dry rot fungusSerpula lacrymansis reviewed. The fungus causes severe damage to construction wood in temperate regions worldwide. Multi-locus genetic analyses have shown thatS. lacrymansincludes two cryptic species currently referred to as var.shastensisand var.lacrymans. Both lineages occur naturally in high altitude regions, but var.lacrymanshas also spread from its natural range in Asia, and established itself in indoor environments in temperate regions worldwide. Japan was apparently colonized independently from Europe, North and South America by var.lacrymans. The population found in Australia and New Zealand seems to represent a mixture of the Japanese and the Euro-American lineages. Little genetic variation exists in the founder populations of var.lacrymansworldwide. Due to the introduction of a limited number ofvic(vegetative incompatibility) alleles into the founder populations, genetically different individuals often cannot recognize self from non-self through the vegetative incompatibility response. Moreover, only a low number of MAT (mating) alleles seem to be present in Europe. Genetic analyses of the European and Japanese indoor populations have shown thatS. lacrymansmainly spreads sexuallyviabasidiospores. Surprisingly, an excess of heterozygotes has been observed in the founder populations. This could be due to heterozygote advantage caused by a limited number of MAT alleles. Recent analyses of theS. lacrymansgenome provide new opportunities for further research on the dry rot fungus.
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| 20. |
- Högberg, Nils, et al.
(författare)
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Microsatellite markers for the ectomycorrhizal basidiomycete Lactarius mammosus
- 2009
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Ingår i: Molecular Ecology Resources. - : Wiley. - 1755-098X .- 1755-0998. ; 9, s. 1008-1010
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Tidskriftsartikel (refereegranskat)abstract
- We report the characterization of polymorphic microsatellite markers in the ectomycorrhizal fungus Lactarius mammosus Fr. Two enrichment protocols were used to isolate microsatellite loci and polymorphism was explored within 31 sporocarps originating from a forest site in northern Sweden. We found nine variable microsatellite loci with the number of alleles per locus varying between 2 and 5, and expected heterozygosities ranging from 0 to 0.84. These loci are available for the analysis of genetic structure and gene flow in L. mammosus populations.
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| 21. |
- Högberg, Nils
(författare)
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Niche differentiation and evolution of the wood decay machinery in the invasive fungus Serpula lacrymans
- 2020
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Ingår i: ISME Journal. - : Springer Science and Business Media LLC. - 1751-7362 .- 1751-7370. ; 15, s. 592-604
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Tidskriftsartikel (refereegranskat)abstract
- Ecological niche breadth and the mechanisms facilitating its evolution are fundamental to understanding adaptation to changing environments, persistence of generalist and specialist lineages and the formation of new species. Woody substrates are structurally complex resources utilized by organisms with specialized decay machinery. Wood-decaying fungi represent ideal model systems to study evolution of niche breadth, as they vary greatly in their host range and preferred decay stage of the substrate. In order to dissect the genetic basis for niche specialization in the invasive brown rot fungusSerpula lacrymans, we used phenotyping and integrative analysis of phylogenomic and transcriptomic data to compare this species to wild relatives in the Serpulaceae with a range of specialist to generalist decay strategies. Our results indicate specialist species have rewired regulatory networks active during wood decay towards decreased reliance on enzymatic machinery, and therefore nitrogen-intensive decay components. This shift was likely accompanied with adaptation to a narrow tree line habitat and switch to a pioneer decomposer strategy, both requiring rapid colonization of a nitrogen-limited substrate. Among substrate specialists with narrow niches, we also found evidence for pathways facilitating reversal to generalism, highlighting how evolution may move along different axes of niche space.
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| 22. |
- Högberg, Nils
(författare)
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Two invasive populations of the dry rot fungus Serpula lacrymans show divergent population genetic structures
- 2010
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Ingår i: Molecular Ecology. - 0962-1083 .- 1365-294X. ; 19, s. 706-715
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Tidskriftsartikel (refereegranskat)abstract
- The dry rot fungus Serpula lacrymans is a devastating basidiomycete occurring in wooden constructions in temperate regions worldwide. In this study, we compare the genetic structures of two invasive populations from Europe and Japan. Microsatellite data from 14 loci and DNA sequences from four loci demonstrated that the two populations were highly differentiated. Significant isolation by distance effect was observed in Europe and Japan. Higher genetic variation was observed within the Japanese population than within the European population, corresponding with the observed higher richness of vegetative compatibility types in Japan, indicating that there has been a higher level of gene flow from the Asian source populations to Japan than to Europe. The European population is genetically more homogenous with only six detected vegetative compatibility types. Various tests indicate that both the European and the Japanese populations have gone through population bottlenecks prior to population expansion. No identical multi-locus genotypes were observed within Japan and very few within Europe, indicating limited clonal dispersal. Deviations from Hardy Weinberg expectations were observed both in Europe and Japan and heterozygote excess were observed at several loci, especially in Europe. Possible explanations for this pattern are discussed.
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| 23. |
- Ihrmark, Katarina, et al.
(författare)
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The Plant Cell Wall-Decomposing Machinery Underlies the Functional Diversity of Forest Fungi
- 2011
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 333, s. 762-765
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Tidskriftsartikel (refereegranskat)abstract
- Brown rot decay removes cellulose and hemicellulose from wood-residual lignin contributing up to 30% of forest soil carbon-and is derived from an ancestral white rot saprotrophy in which both lignin and cellulose are decomposed. Comparative and functional genomics of the "dry rot" fungus Serpula lacrymans, derived from forest ancestors, demonstrated that the evolution of both ectomycorrhizal biotrophy and brown rot saprotrophy were accompanied by reductions and losses in specific protein families, suggesting adaptation to an intercellular interaction with plant tissue. Transcriptome and proteome analysis also identified differences in wood decomposition in S. lacrymans relative to the brown rot Postia placenta. Furthermore, fungal nutritional mode diversification suggests that the boreal forest biome originated via genetic coevolution of above- and below-ground biota.
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| 24. |
- Jämtgård, Sandra, et al.
(författare)
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Förstudie kring miljöanpassad skogsgödsling för ökad skoglig tillväxt - slutrapportering av regeringsuppdrag
- 2023
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- I denna rapport redovisas resultatet från regeringsuppdraget Förstudie kring miljöanpassad skogsgödsling för ökad skoglig tillväxt (SLU.ua.2022.1.1.1–2974) som givits i uppdrag till SLU. Uppdragets syfte är att bidra till att stärka bioekonomins roll i klimatomställningen. SLU ska ge förslag på hur formerna för skogsgödsling kan utvecklas så att en större andel av skogsmarken kan gödslas än vad som sker idag samtidigt som miljöpåverkan och effekter på biologisk mångfald minimeras, samt identifiera eventuella ytterligare analysbehov. Förslagen ska inkludera effekterna på skoglig tillväxt, påverkan på skogen som kolsänka och potentiell avverkning både i ett kortare perspektiv till 2030 och i ett längre perspektiv till 2050.
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| 25. |
- Kancherla, Reddy Prakash, et al.
(författare)
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Draft genome of the brown-rot fungus Fomitopsis pinicola GR9-4
- 2017
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Ingår i: Data in Brief. - : Elsevier BV. - 2352-3409. ; 15, s. 496-500
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Tidskriftsartikel (refereegranskat)abstract
- Basidiomycete brown-rot fungi have a huge importance for wood decomposition and thus the global carbon cycle. Here, we present the genome sequence of Fomitopsis pinicolaGR9-4 which represent different F. pinicola clade than the previously sequenced North American isolate FP-58527 SS1. The genome was sequenced by using a paired-end sequence library of Illumina and a 2.5k and 5k mate-pair library (ABI SOLiD). The final assembly adds up to a size of 45 Mb (including gaps between contigs), with a GC-content of 56%. The gene prediction resulted in 13,888 gene models. The genome sequence will be used as a basis for understanding population genomics, genome-wide association studies and wood decay mechanisms of this brown-rot fungus.
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