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- Ehrlund, A, et al.
(författare)
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The cell-type specific transcriptome in human adipose tissue and influence of obesity on adipocyte progenitors
- 2017
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Ingår i: Scientific data. - : Springer Science and Business Media LLC. - 2052-4463. ; 4, s. 170164-
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Tidskriftsartikel (refereegranskat)abstract
- Obesity affects gene expression and metabolism of white adipose tissue (WAT), which results in insulin resistance (IR) and type 2 diabetes. However, WAT is a heterogeneous organ containing many cell types that might respond differently to obesity-induced changes. We performed flow cytometry sorting and RNA expression profiling by microarray of major WAT cell types (adipocytes, CD45−/CD31−/CD34+ progenitors, CD45+/CD14+ monocytes/ macrophages, CD45+/CD14− leukocytes), which allowed us to identify genes enriched in specific cell fractions. Additionally, we included adipocytes and adipocyte progenitor cells obtained from lean and obese individuals. Taken together, we provide a detailed gene expression atlas of major human adipose tissue resident cell types for clinical/basic research and using this dataset provide lists of cell-type specific genes that are of interest for metabolic research.
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- Kulyte, A, et al.
(författare)
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Additive effects of microRNAs and transcription factors on CCL2 production in human white adipose tissue
- 2014
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Ingår i: Diabetes. - : American Diabetes Association. - 1939-327X .- 0012-1797. ; 63:4, s. 1248-1258
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Tidskriftsartikel (refereegranskat)abstract
- Adipose tissue inflammation is present in insulin-resistant conditions. We recently proposed a network of microRNAs (miRNAs) and transcription factors (TFs) regulating the production of the proinflammatory chemokine (C-C motif) ligand-2 (CCL2) in adipose tissue. We presently extended and further validated this network and investigated if the circuits controlling CCL2 can interact in human adipocytes and macrophages. The updated subnetwork predicted that miR-126/-193b/-92a control CCL2 production by several TFs, including v-ets erythroblastosis virus E26 oncogene homolog 1 (avian) (ETS1), MYC-associated factor X (MAX), and specificity protein 12 (SP1). This was confirmed in human adipocytes by the observation that gene silencing of ETS1, MAX, or SP1 attenuated CCL2 production. Combined gene silencing of ETS1 and MAX resulted in an additive reduction in CCL2 production. Moreover, overexpression of miR-126/-193b/-92a in different pairwise combinations reduced CCL2 secretion more efficiently than either miRNA alone. However, although effects on CCL2 secretion by co-overexpression of miR-92a/-193b and miR-92a/-126 were additive in adipocytes, the combination of miR-126/-193b was primarily additive in macrophages. Signals for miR-92a and -193b converged on the nuclear factor-κB pathway. In conclusion, TF and miRNA-mediated regulation of CCL2 production is additive and partly relayed by cell-specific networks in human adipose tissue that may be important for the development of insulin resistance/type 2 diabetes.
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- Boyle, Mark, et al.
(författare)
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Excitation dynamics of Rydberg states in C60
- 2005
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Ingår i: European Physical Journal D. - : Springer Science and Business Media LLC. - 1434-6060 .- 1434-6079. ; 36, s. 339-351
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Tidskriftsartikel (refereegranskat)abstract
- The electron and nuclear dynamics of C60 fullerenes irradiated with femtosecond laser pulses are investigated with photoelectron and photoion spectroscopy. The focus of this work is the detailed exploration of the population mechanism of Rydberg levels within the excitation process of neutral C60. The effect of excitation wavelength, intensity, chirp, and polarization on the kinetic energy distribution of photoelectrons in single-pulse experiments gives first insight into the underlying processes. In combination with time-resolved two-color pump-probe spectroscopy depending on either pump, or probe pulse intensity, a more complete picture of the interaction can be drawn. The results point towards a very interesting but nevertheless complex behavior including four steps: (i) non-adiabatic multielectron excitation of the HOMO (hu) → LUMO+1 (t1g) transition; (ii) thermalization within the hot electron cloud on a time scale below 100fs, followed by a coupling of energy to vibrational modes of the molecule via doorway state(s); (iii) population of electronically excited Rydberg states by multiphoton absorption, and (iv) single photon ionization from the excited Rydberg states. This excitation process results in a characteristic sequence of photoelectron lines in the photoemission spectra. The comparison of the experimental results with recent theoretical work gives convincing evidence that non-adiabatic multielectron dynamics (NMED) plays a key role for the understanding of the response of C60 to short-pulse laser radiation.
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- Boyle, Mark, et al.
(författare)
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Two colour pump-probe study and internal energy dependence of Rydberg state excitation
- 2004
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Ingår i: Physical Review A. - 1050-2947. ; 70
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Tidskriftsartikel (refereegranskat)abstract
- Excitation of Rydberg states in isolated C60 is studied by time-resolved photoelectron spectroscopy in a femtosecond two-color pump-probe experiment. The relaxation time for electron-electron interaction is determined to be approximately 100 fs with the t1g(LUMO + 1) orbital being considered to define the doorway state in a nonadiabatic multielectron excitation process. The internal energy stored in vibrational modes of the C60 at 770 K is found to support the excitation process very efficiently while in "cold" C60 (80 K), no significant Rydberg population is detected.
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- El-Zanati, S., et al.
(författare)
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Partitions of the 8-Dimensional Vector Space Over GF(2)
- 2010
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Ingår i: Journal of combinatorial designs (Print). - : Wiley. - 1063-8539 .- 1520-6610. ; 18:6, s. 462-474
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Tidskriftsartikel (refereegranskat)abstract
- Let V=V(n,q) denote the vector space of dimension n over GF(q). A set of subspaces of V is called a partition of V if every nonzero vector in V is contained in exactly one subspace of V. Given a. partition P of V with exactly a(i) subspaces of dimension i for 1 <= i <= n, we have Sigma(n)(i=1) a(i)(q(i)-1) = q(n)-1, and we call the n-tuple (a(n), a(n-1), ..., a(1)) the type of P. In this article we identify all 8-tuples (a(8), a(7), ..., a(2), 0) that are the types of partitions of V(8,2).
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- Heden, Olof, et al.
(författare)
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On the type(s) of minimum size subspace partitions
- 2014
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Ingår i: Discrete Mathematics. - : Elsevier BV. - 0012-365X .- 1872-681X. ; 332, s. 1-9
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Tidskriftsartikel (refereegranskat)abstract
- Let V = V(kt + r, q) be a vector space of dimension kt + r over the finite field with q elements. Let sigma(q)(kt + r, t) denote the minimum size of a subspace partition P of V in which t is the largest dimension of a subspace. We denote by n(di) the number of subspaces of dimension d(i) that occur in P and we say [d(1)(nd1),..., d(m)(ndm)] is the type of P. In this paper, we show that a partition of minimum size has a unique partition type if t + r is an even integer. We also consider the case when t + r is an odd integer, but only give partial results since this case is indeed more intricate.
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- Heden, Olof, et al.
(författare)
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The supertail of a subspace partition
- 2013
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Ingår i: Designs, Codes and Cryptography. - : Springer Science and Business Media LLC. - 0925-1022 .- 1573-7586. ; 69:3, s. 305-316
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Tidskriftsartikel (refereegranskat)abstract
- Let V = V(n, q) be a vector space of dimension n over the finite field with q elements, and let d (1) < d (2) < ... < d (m) be the dimensions that occur in a subspace partition of V. Let sigma (q) (n, t) denote the minimum size of a subspace partition of V, in which t is the largest dimension of a subspace. For any integer s, with 1 < s a parts per thousand currency sign m, the set of subspaces in of dimension less than d (s) is called the s-supertail of . The main result is that the number of spaces in an s-supertail is at least sigma (q) (d (s) , d (s-1)).
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- Stark, B, et al.
(författare)
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Results after resection of intraoral cancer and reconstruction with the free radial forearm flap
- 1998
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Ingår i: ORL. - : S. Karger AG. - 0301-1569 .- 1423-0275. ; 60:4, s. 212-217
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Tidskriftsartikel (refereegranskat)abstract
- Between 1989 and 1996, 47 patients with an intraoral squamous cell carcinoma underwent tumor resection and reconstruction with a free volar forearm flap. Tumor resection and neck dissection were performed by a head and neck surgeon and the free tissue transfer by a plastic surgeon. Preoperative radiation therapy was given to 44 of 47 patients and postoperative radiation therapy to 2. One other patient was not irradiated. There were 15 females and 32 males, with a mean age of 61 years. The primary site of the cancer was the tongue in 15 cases, the floor of the mouth in 15, the tonsil in 10, the bucca in 3 and the retromolar trigone in 4 cases. The flap was harvested from the left forearm in 34 and from the right in 13 patients. The mean operation time was 10 h (range 6–20) and the mean intraoperative bleeding was 486 ml (250–2,500). Forty-four of 46 flaps healed completely. Twelve revisions on 9 free flaps were performed between 6 h and 6 days postoperatively. Overall revisional surgery was done in 9 of 47 cases (19%). Two flaps could not be saved (4%). Infections/fistulas in the neck occurred in 11 of 46 cases (24%). Complete healing of the donor site occurred at a mean of 2.5 months (1–5). Nine patients developed complications at the donor site, 3 hematomas and 6 superficial infections (19%). Twenty-seven of 47 patients are still alive (57%). The mean survival time was 49 months (16–71).
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