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- Johansson, G, et al.
(författare)
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Underreporting of energy intake in repeated 24-hour recalls related to gender, age, weight status, day of interview, educational level, reported food intake, smoking habits and area of living.
- 2001
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Ingår i: Public Health Nutrition. - 1368-9800 .- 1475-2727. ; 4:4, s. 919-927
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: The aims of the present study were (1) to evaluate the degree to which underreporting of energy intake by repeated 24-hour recalls was related to gender, age, weight status, day of interview, educational level, smoking habits and area of living, and (2) to compare the dietary characteristics of underreporters with those of others. DESIGN: Cross-sectional study. Ten 24-hour recalls were performed during a one-year period. SETTING: The Västerbotten intervention programme of cardiovascular disease and diabetes in Northern Sweden. SUBJECTS: Ninety-four men and 99 women in four age groups: 30, 40, 50 and 60 years. RESULTS: The prevalence of men and women with a food intake level (FIL; reported energy intake divided by estimated basal metabolic rate) below 1.2 was 44% and 47%, respectively. The youngest age group had higher FIL values than the oldest age group for both men (1.5 versus 1.1) and women (1.4 versus 1.1). The prevalence and magnitude of underreporting were directly related to body mass index (BMI; correlation coefficient: -0.47 (men) and -0.55 (women)). Smokers had a lower FIL value (1.1) than non-smokers (1.3). The nutrient density was lower for the group with high FIL values for protein and calcium and higher for fat and sucrose. The upper FIL group often had higher intake frequencies and larger portion sizes than the lower FIL group. CONCLUSIONS: Underreporting of energy intake is prevalent when 24-hour recalls are used, but the prevalence differs between sub-groups in the population. BMI was the main predictor of underreporting but also old age and smoking seem to contribute in this aspect. Socially desirable food items were not underreported to the same extent as socially undesirable food items. The intake frequencies and portion sizes partly explained the differences in FIL.
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| 4. |
- Johansson, LB-Å, et al.
(författare)
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Phase equilibria and formation of vesicles of dioleoylphosphatidylcholine in glycerol/water mixtures
- 1993
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Ingår i: Biochimica et Biophysica Acta - Biomembranes. - 0005-2736 .- 1879-2642. ; 1149, s. 285-291
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Tidskriftsartikel (refereegranskat)abstract
- The lipid 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) forms a lamellar liquid crystalline phase (La) in arbitrary mixtures of glycerol and water. The phase has been characterized by means of X-ray diffraction, 3IP-NMR spectroscopy and differential scanning calorimetry (DSC). In the La state, and for DOPC concentrations greater than 50% (w/w), the thickness of the lipid bilayer decreases, while the area of the polar head group increases with increasing glycerol concentration. The phase transition from gel to La state occurs in the range of 240 to 260 K. Contrary to a previous (McDaniel, R.V., McIntosh, T.J. and Simon, S.A. (1983) Biochim. Biophys. Acta 731, 97) study of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) we find that in the gel state, the thickness of the DOPC lipid bilayer is greater than that in the La state. This suggests that in the gel state, the lipid acyl chains of DOPC are in extended configuration. The lamellar phase reaches its maximum swelling at about 50% (w/w) of DOPC. At lower DOPC concentrations a two-phase system is formed where the lamellar phase exists in equilibrium with excess of solvent. Unilamellar vesicles can be prepared from a diluted suspension of the lamellar phase either by using the sonicator or extruder technique. We show this by means of 3IP-NMR, EPR and fluorescence spectroscopy. The mean radius of the vesicles, prepared by a sonicator, has been determined at different glycerol/water mixtures. It is found to decrease continously from 100 A at 100% water to a minimum of 75 Å at about 50% water in the solvent mixture. By further decreasing the water content in the solution, the radius rapidly increases, and a mean radius of 450 Å is estimated at a water content of 10%. The rotational relaxation times of a fluorescent probe and two EPR spin probes, solubilized in DOPC vesicles, have been measured at different glycerol/water mixtures. It is found that the rotational rates are always much slower in the systems containing glycerol.
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| 6. |
- Marushchak, Denys, et al.
(författare)
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Fluorescence Depolarisation Studies of Filamentous Actin Analysed with a Genetic Algorithm
- 2007
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Ingår i: Biophysical Journal. - : CellPress. - 0006-3495 .- 1542-0086. ; 93:9, s. 3291-3299
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Tidskriftsartikel (refereegranskat)abstract
- A new method, in which a genetic algorithm was combined with Brownian dynamics and Monte Carlo simulations, was developed to analyze fluorescence depolarization data collected by the time-correlated single photon-counting technique. It was applied to studies of BODIPY-labeled filamentous actin (F-actin). The technique registered the local order and reorienting motions of the fluorophores, which were covalently coupled to cysteine 374 (C374) in actin and interacted by electronic energy migration within the actin polymers. Analyses of F-actin samples composed of different fractions of labeled actin molecules revealed the known helical organization of F-actin, demonstrating the usefulness of this technique for structure determination of complex protein polymers. The distance from the filament axis to the fluorophore was found to be considerably less than expected from the proposed position of C374 at a high filament radius. In addition, polymerization experiments with BODIPY-actin suggest a 25-fold more efficient signal for filament formation than pyrene-actin.
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| 10. |
- Andersson, U.B., et al.
(författare)
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Characterization of the Paleoproterozoic Mantle beneath the Fennoscandian Shield : Geochemistry and Isotope Geology (Nd, Sr) of ~ 1.8 Ga Mafic Plutonic Rocks from the Transscandinavian Igneous Belt in Southeast Sweden
- 2007
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Ingår i: International Geology Review. - : Informa UK Limited. - 0020-6814 .- 1938-2839. ; 49:7, s. 587-625
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Forskningsöversikt (refereegranskat)abstract
- Geochemistry and Sr-Nd isotope geochemistry of ca. 1.8 Ga, mafic intrusions of the Transscandinavian Igneous Belt (TIB-1) in the Fennoscandian shield were studied in southeastern Sweden. These rocks show LILE-LREE-enriched, HFSE-depleted, calc-alkaline, continental arc signatures in the north, grading to slightly less enriched, oceanic affinities southward.εNd(1.80) values range from +2.0 to +0.7 and 87Sr/86Sr(1.80) from 0.7022 to 0.7029 (with one outlier at 0 and 0.7033), without correlation to fractionation (e.g., Mg#) or crustal contamination, indicating sources that are mildly depleted. The most depleted ratios occur in the south, trending with the geochemistry toward more enriched compositions northward. The sources represent depleted mantle wedge material that was subjected to enrichment not long before (TDM ca. 2.0 Ga), i.e., during the preceding arc subduction (2.1-1.82 Ga), and/or during the TIB-1 magmatism itself, by hydrous fluids with a sediment and/or melt input increasing northward. The TIB-1 magmatism occurred above a south(west)ward-retreating subduction zone along the continental margin of the juvenile Svecofennian continent at 1.81-1.76 Ga.
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| 16. |
- Berg, Kristian, 1959-, et al.
(författare)
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Kaliningrad : byggnadsvård kan hindra kulturell katastrof
- 2000
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Ingår i: Kulturmiljövård. - Stockholm : Riksantikvarieämbetet. - 1100-4800. ; :1, s. 73-77
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Tidskriftsartikel (populärvet., debatt m.m.)abstract
- -Jag har i hela mitt liv fått höra historien om den underjordiska gömda staden i Kaliningrad. Under ytan av den stad vi nu ser skulle det finnas en annan, med alla de historiska lämningar och rikedomar som detta Königsberg en gång var förknippat med.
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| 17. |
- Berg, Stig, et al.
(författare)
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Åldrandets biologi
- 1980
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Bok (övrigt vetenskapligt/konstnärligt)
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| 18. |
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| 19. |
- Bergström, Fredrik, et al.
(författare)
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Dimers of Dipyrrometheneboron Difluoride (BODIPY) with Light Spectroscopic Applications in Chemistry and Biology
- 2002
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Ingår i: Journal of the American Chemical Society. - : American Chemical Society (ACS). - 0002-7863 .- 1520-5126. ; 124:2, s. 196-204
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Tidskriftsartikel (refereegranskat)abstract
- A ground-state dimer (denoted DI) exhibiting a strong absorption maximum at 477 nm ( = 97 000 M-1cm-1) can form between adjacent BODIPY groups attached to mutant forms of the protein, plasminogen activator inhibitor type 1 (PAI-1). No fluorescence from excited DI was detected. A locally high concentration of BODIPY groups was also achieved by doping lipid phases (micelles, vesicles) with BODIPY-labeled lipids. In addition to an absorption band located at about 480 nm, a new weak absorption band is also observed at ca. 570 nm. Both bands are ascribed to the formation of BODIPY dimers of different conformation (DI and DII). Contrary to DI in PAI-1, the DII aggregates absorbing at 570 nm are emitting light observed as a broad band centered at about 630 nm. The integrated absorption band of DI is about twice that of the monomer, which is compatible with exciton coupling within a dimer. The Förster radius of electronic energy transfer between a BODIPY excited monomer and the ground-state dimer (DI) is 57 ± 2 Å. A simple model of exciton coupling suggests that in DI two BODIPY groups are stacked on top of each other in a sandwich-like configuration with parallel electronic transition dipoles. For DII the model suggests that the S0 S1 transition dipoles are collinear. An explanation for the previously reported (J. Am. Chem. Soc. 1994, 116, 7801) exceptional light spectroscopic properties of BODIPY is also presented. These are ascribed to the extraordinary electric properties of the BODIPY chromophore. First, changes of the permanent electric dipole moment ( -0.05 D) and polarizability (-26 × 10-40 C m2 V-1) between the ground and the first excited states are small. Second, the S0 S1 electronic transition dipole moments are perpendicular to .
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| 22. |
- Broeren, Jurgen, et al.
(författare)
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Information and communication technology : a person-centered approach to stroke care
- 2012
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Ingår i: Proceedings of the 9th International Conference on Disability, Virtual Reality and Associated Technologies. - Readings : University of Readings. - 9780704915459 ; , s. 329-335
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Konferensbidrag (refereegranskat)abstract
- This report describes the possibilities of information and communication technology (ICT) in stroke care, addressing a person-centered care (PCC) approach. Attention is paid to user involvement, design, videogames, and communication between health care professionals mutually as well as with patients, and how to share performance data with an electronic health record. This is the first step towards a supportive ICT system that facilitates interoperability, making healthcare information and services available to citizen’s across organizational boundaries.
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| 23. |
- Burghart, Armin, et al.
(författare)
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Energy transfer cassettes based on BODIPY® dyes
- 2000
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Ingår i: Chemical Communications. - : Royal Society of Chemistry (RSC). - 1359-7345 .- 1364-548X. ; :2203-4
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Tidskriftsartikel (refereegranskat)abstract
- The donor–acceptor dye cassettes 1–4 were designed to capture energy at a single wavelength and to convert it to well-resolved, intense fluorescence emissions; in practice, Stokes shifts of 40–148 nm, quantum yields of 0.12–0.60, and efficient energy transfer was demonstrated.
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| 25. |
- Chorell, Erik, et al.
(författare)
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Design and Synthesis of Fluorescent Pilicides and Curlicides : Bioactive Tools to Study Bacterial Virulence Mechanisms
- 2012
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Ingår i: Chemistry - A European Journal. - Berlin : Wiley-VCH Verlagsgesellschaft. - 0947-6539 .- 1521-3765. ; 18:15, s. 4522-4532
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Tidskriftsartikel (refereegranskat)abstract
- Pilicides and curlicides are compounds that block the formation of the virulence factors pili and curli, respectively. To facilitate studies of the interaction between these compounds and the pili and curli assembly systems, fluorescent pilicides and curlicides have been synthesized. This was achieved by using a strategy based on structure-activity knowledge, in which key pilicide and curlicide substituents on the ring-fused dihydrothiazolo 2-pyridone central fragment were replaced by fluorophores. Several of the resulting fluorescent compounds had improved activities as measured in pili- and curli-dependent biofilm assays. We created fluorescent pilicides and curlicides by introducing coumarin and 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) fluorophores at two positions on the peptidomimetic pilicide and curlicide central fragment. Fluorescence images of the uropathogenic Escherichia coli (UPEC) strain UTI89 grown in the presence of these compounds shows that the compounds are strongly associated with the bacteria with a heterogeneous distribution.
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