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1.	Thery, C, et al. (author) Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines 2018 In: Journal of extracellular vesicles. - : Wiley. - 2001-3078. ; 7:1, s. 1535750- Journal article (peer-reviewed)
2.	Escutenaire, Sophie, et al. (author) SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses 2007 In: Archives of Virology. - : Springer Science and Business Media LLC. - 0304-8608 .- 1432-8798. ; 152:1, s. 41-58 Journal article (peer-reviewed)abstract Coronaviruses are etiologic agents of respiratory and enteric diseases in humans and in animals. In this study, a one-step real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on SYBR Green chemistry and degenerate primers was developed for the generic detection of coronaviruses. The primers, designed in the open reading frame 1b, enabled the detection of 32 animal coronaviruses including strains of canine coronavirus, feline coronavirus, transmissible gastroenteritis virus (TGEV), bovine coronavirus (BCoV), murine hepatitis virus (MHV) and infectious bronchitis virus (IBV). A specific amplification was also observed with the human coronaviruses (HCoV) HCoV-NL63, HCoV-OC43, HCoV-229E and severe acute respiratory syndrome coronavirus (SARS-CoV). The real-time RT-PCR detected down to 10 cRNA copies from TGEV, BCoV, SARS-CoV and IBV. In addition, the assay exhibited a high sensitivity and specificity on clinical samples from different animal species. The developed assay represents a potential tool for laboratory diagnostics and for detecting still uncharacterized coronaviruses.

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Type of publication: journal article (2)

Type of content: peer-reviewed (2)

Author/Editor: Chen, S. (1); Jenster, G (1); Antoniou, A (1); Park, J (1); Sharma, S. (1); Yin, H. (1); show more...; Das, S. (1); Rak, J. (1); Schneider, R. (1); Krause, M. (1); Moller, A (1); Ortiz, A (1); Weiss, DJ (1); Leszczynska, A. (1); Ostrowski, M. (1); Blomberg, Jonas (1); Mohamed, Nahla (1); Xu, J (1); Sahoo, S. (1); Belak, Sandor (1); Buch, S (1); Nejsum, P (1); Gabrielsson, S (1); Lötvall, Jan, 1956 (1); Cheng, L (1); Shiba, K (1); Wheelock, AM (1); Zheng, L (1); Kaur, S (1); Scott, A (1); Nawaz, Muhammad (1); Witwer, KW (1); Hunt, S (1); Chen, CC (1); Gardiner, C (1); Lim, R (1); Mullier, F (1); Baharvand, H (1); Huber, V. (1); Zhao, ZZ (1); Anderson, JD (1); Ivanova, A (1); Gustafson, D (1); Pulliam, L. (1); Tahara, H (1); Vader, P (1); Gilbert, C (1); Llorente, A (1); Rome, S (1); Perut, F (1); show less...

University: University of Gothenburg (1); Uppsala University (1); Lund University (1); Karolinska Institutet (1)

Language: English (2)

Research subject (UKÄ/SCB): Natural sciences (1); Medical and Health Sciences (1)

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