| 1. |
- Ou, Pan, et al.
(författare)
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Gasotransmitter Regulation of Phosphatase Activity in Live Cells Studied by Three-Channel Imaging Correlation
- 2019
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Ingår i: Angewandte Chemie International Edition. - : WILEY-V C H VERLAG GMBH. - 1433-7851 .- 1521-3773. ; 58:8, s. 2261-2265
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Tidskriftsartikel (refereegranskat)abstract
- Enzyme activity in live cells is dynamically regulated by small-molecule transmitters for maintaining normal physiological functions. A few probes have been devised to measure intracellular enzyme activities by fluorescent imaging, but the study of the regulation of enzyme activity via gasotransmitters in situ remains a long-standing challenge. Herein, we report a three-channel imaging correlation by a single dual-reactive fluorescent probe to measure the dependence of phosphatase activity on the H2S level in cells. The two sites of the probe reactive to H2S and phosphatase individually produce blue and green fluorescent responses, respectively, and resonance energy transfer can be triggered by their coexistence. Fluorescent analysis based on the three-channel imaging correlation shows that cells have an ideal level of H2S to promote phosphatase activity up to its maximum. Significantly, a slight deviation from this H2S level leads to a sharp decrease of phosphatase activity. The discovery further strengthens our understanding of the importance of H2S in cellular signaling and in various human diseases.
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| 2. |
- Yang, Guanqing, et al.
(författare)
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A Multi-responsive Fluorescent Probe Reveals Mitochondrial Nucleoprotein Dynamics with Reactive Oxygen Species Regulation through Super-resolution Imaging
- 2020
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Ingår i: Angewandte Chemie International Edition. - : WILEY-V C H VERLAG GMBH. - 1433-7851 .- 1521-3773. ; 59:37, s. 16154-16160
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Tidskriftsartikel (refereegranskat)abstract
- Understanding the biomolecular interactions in a specific organelle has been a long-standing challenge because it requires super-resolution imaging to resolve the spatial locations and dynamic interactions of multiple biomacromolecules. Two key difficulties are the scarcity of suitable probes for super-resolution nanoscopy and the complications that arise from the use of multiple probes. Herein, we report a quinolinium derivative probe that is selectively enriched in mitochondria and switches on in three different fluorescence modes in response to hydrogen peroxide (H2O2), proteins, and nucleic acids, enabling the visualization of mitochondrial nucleoprotein dynamics. STED nanoscopy reveals that the proteins localize at mitochondrial cristae and largely fuse with nucleic acids to form nucleoproteins, whereas increasing H(2)O(2)level leads to disassociation of nucleic acid-protein complexes.
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| 3. |
- Gong, Zheng, et al.
(författare)
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A targetable fluorescent probe for detecting mitochondrial viscosity in live cells by using fluorescence lifetime imaging
- 2024
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Ingår i: New Journal of Chemistry. - : ROYAL SOC CHEMISTRY. - 1144-0546 .- 1369-9261.
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Tidskriftsartikel (refereegranskat)abstract
- Intracellular viscosity plays an important role in regulating cellular morphology and physiology and is closely related to a host of diseases. Especially, the changes in mitochondrial viscosity will cause some common diseases such as hyperlipidemia, Alzheimer's disease and cancer. In this work, we report the design of a red-emissive molecular rotor for the detection of mitochondrial viscosity in live cells. The probe showed fascinating performance, such as specific targeting to mitochondria, high sensitivity to viscosity, and rapid fluorescence response, especially the dual response mode of fluorescence intensity and fluorescence lifetime. By using this probe, we realized monitoring of the mitochondrial viscosity variations in live cells under different physiological processes. Our study offers an opportunity to discover potential tools for mitochondria-related physiology and pathology investigation. A D-pi-A typed fluorescence lifetime probe for sensitively detecting viscosity has been designed and synthesized. BSOH has been successfully applied to real-time monitoring mitochondrial viscosity in live cells by fluorescence lifetime imaging.
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| 4. |
- Shen, Jie, et al.
(författare)
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Real-time monitoring of lipid droplets growth via the fusion with fluorescent dye-labeled adiposomes
- 2020
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Ingår i: Dyes and pigments. - : ELSEVIER SCI LTD. - 0143-7208 .- 1873-3743. ; 182
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Tidskriftsartikel (refereegranskat)abstract
- Investigating lipid droplets (LDs) behaviours is essential to deeply understand the physiology of LDs, such as their growths, movements, fusion/division, and autophagy. Among these behaviours, the growth of LDs is one of the most difficult to track due to the very subtle morphology evolution in a short time window. The major obstacle is that conventional LDs-specific dyes with low photostability cannot indicate the LDs size change. To address this issue, we synthesize a hydrophobic and photostable fluorescent dye (TPA-AD) and load it into the neutral lipid micelles (as artificial adiposomes). The highly hydrophobic TPA-AD enables the specific accumulation into intracellular LDs and the ready loading artificial adiposomes. When the intracellular LDs take TPA-AD-labeled adiposomes, by fusion, the sizes of LDs gradually grow, and LDs are simultaneously lighted up by the fluorescence of TPA-AD. Importantly, the high photostability of TPA-AD ensures the enhanced fluorescence signals. The finding here will further strengthen the understanding of LDs dynamics and fat metabolism.
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| 5. |
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| 6. |
- Smith, Andy, et al.
(författare)
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Institutionalizing HCI in Asia
- 2007
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Ingår i: Human-Computer Interaction - INTERACT 2007, Pt 2, Proceedings. - Berlin : Springer. ; , s. 85-99
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Konferensbidrag (refereegranskat)abstract
- In this paper we investigate the problems and potential solutions to the effective establishment of HCI and usability in India and China. Our discussion is motivated by five years of collaboration with relevant bodies in both countries through EU-funded projects encouraging the development of a usability culture in academic and industrial sectors. In order to contribute to socially-responsible interaction in these countries the ’institutionalization’ of HCI is necessary. For us, this involves three elements: firstly an appropriation of HCI concepts and methods to suit the local country / culture, secondly the forming of a national organization around the reshaped discipline that can actively promote HCI in industry and academia and establish links with local national organizations, and thirdly the roll-out of effective usability practice in industry. Some efforts made in this regard are briefly outlined.
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