1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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2. |
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3. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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4. |
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5. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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6. |
- Ablikim, M., et al.
(författare)
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Amplitude analysis of D0 → K -π+π+π-
- 2017
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Ingår i: Physical Review D. - 2470-0010 .- 2470-0029. ; 95:7
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Tidskriftsartikel (refereegranskat)abstract
- We present an amplitude analysis of the decay D0 → K -π+π+π- based on a data sample of 2.93 fb−1 acquired by the BESIII detector at the ψ(3770) resonance. With a nearly background free sample of about 16000 events, we investigate the substructure of the decay and determine the relative fractions and the phases among the different intermediate processes. Our amplitude model includes the two-body decays D0 → ¯K*0ρ0, D0 → K−a+1(1260) and D0 → K−1(1270)π+, the three-body decays D0 →¯K*0π+π− and D0 → K−π+ρ0, as well as the four-body nonresonant decay D0 → K−π+π+π−. The dominant intermediate process is D0 → K−a+1(1260), accounting for a fit fraction of 54.6%.
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7. |
- Ablikim, M., et al.
(författare)
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Confirmation of a charged charmoniumlike state Z(c)(3885)(-/+) in e(+)e(-) -> pi(+/-) (D(D)over-bar*)(-/+) with double D tag
- 2015
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Ingår i: Physical Review D. - 1550-7998 .- 1550-2368. ; 92:9
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Tidskriftsartikel (refereegranskat)abstract
- We present a study of the process e(+)e(-) -> pi(+/-) (D (D) over bar*)(-/+) using data samples of 1092 pb(-1) at root s = 4.23 GeV and 826 pb(-1) at root s = 4.26 GeV collected with the BESIII detector at the BEPCII storage ring. With full reconstruction of the D meson pair and the bachelor pi(+) in the final state, we confirm the existence of the charged structure Z(c) (3885)(-/+) in the (D (D) over bar*)(-/+) system in the two isospin processes e(+)e(-) -> pi(+DD)-D-0*(-) and e(+)e(-) -> pi+D-D*(0). By performing a simultaneous fit, the statistical significance of Zc(3885)(-/+) signal is determined to be greater than 10 sigma, and its pole mass and width are measured to be M-pole = (3881.7 +/- 1.6(stat) +/- 1.6(syst)) MeV/c(2) and Gamma(pole) = (26.6 +/- 2.0(stat) +/- 2.1(syst)) MeV, respectively. The Born cross section times the (D (D) over bar*)(-/+) branching fraction (sigma(e(+)e(-) -> pi(+/-)Z(c)(3885)(-/+)) x Br(Z(c)(3885)(-/+) -> (D (D) over bar*)(-/+) )) is measured to be (141.6 +/- 7.9(stat) +/- 12.3(syst)) pb at root s = 4.23 GeV and (108.4 +/- 6.9(stat) +/- 8.8(syst)) pb at root s = 4.26 GeV. The polar angular distribution of the pi(+) - Z(c)(3885)(-/+) system is consistent with the expectation of a quantum number assignment of J(P) = 1(+) for Z(c)(3885)(-/+).
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8. |
- Ablikim, M., et al.
(författare)
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Determination of the Spin and Parity of the Z(c)(3900)
- 2017
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Ingår i: Physical Review Letters. - : AMER PHYSICAL SOC. - 0031-9007 .- 1079-7114. ; 119:7
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Tidskriftsartikel (refereegranskat)abstract
- The spin and parity of the Z(c)(3900)(+/-) state are determined to be J(P) = 1(+) with a statistical significance larger than 7 sigma over other quantum numbers in a partial wave analysis of the process e(+)e(-) -> pi(+)pi(-) J/psi We use a data sample of 1.92 fb(-1) accumulated at root s = 4.23 and 4.26 GeV with the BESIII experiment. When parametrizing the Z(c)(3900)(+/-) with a Flatte-like formula, we determine its pole mass M-pole = (3881.2 +/- 4.2(stat) +/- 52.7(syst)) MeV/c(2) and pole width Gamma(pole) = (51.8 +/- 4.6(stat) +/- 36.0(syst)) MeV. We also measure cross sections for the process e(+)e(-) -> Z(c)(3900)(+)pi(-) + c.c. -> J/psi pi(+)pi(-) and determine an upper limit at the 90% confidence level for the process e(+)e(-) -> Z(c)(4020)(+)pi(-) + c.c. -> J/psi pi(+)pi(-).
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9. |
- Ablikim, M., et al.
(författare)
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First measurement of e(+)e(-) -> pK(S)(0)(n)over-barK(-) + c.c. above open charm threshold
- 2018
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Ingår i: Physical Review D. - 2470-0010 .- 2470-0029. ; 98:3
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Tidskriftsartikel (refereegranskat)abstract
- The process e(+)e(-) -> pK(S)(0)(n) over barK(-) + c.c. and its intermediate processes are studied for the first time, using data samples collected with the BESIII detector at BEPCII at center-of-mass energies of 3.773, 4.008, 4.226, 4.258, 4.358, 4.416, and 4.600 GeV, with a total integrated luminosity of 7.4 fb(-1). The Born cross section of e(+)e(-) -> pK(S)(0)(n) over barK(-) + c.c. is measured at each center-of-mass energy, but no significant resonant structure in the measured cross-section line shape between 3.773 and 4.600 GeV is observed. No evident structure is detected in the pK(-), nK(S)(0), pK(S)(0), nK(+), p (n) over bar, or (KSK-)-K-0 invariant mass distributions except for Lambda(1520). The Born cross sections of e(+)e(-) -> Lambda(1520)(n) over barK(S)(0) + c.c. and e(+)e(-) -> Lambda(1520)(p) over barK(+) + c.c. are measured, and the 90% confidence level upper limits on the Born cross sections of e(+)e(-) -> Lambda(1520)(Lambda) over bar (1520) are determined at the seven center-of-mass energies. There is an evident difference in line shape and magnitude of the measured cross sections between e(+)e(-) -> Lambda(1520)(-> pK(-))(n) over barK(S)(0) and e(+)e(-) -> pK-(Lambda) over bar (1520)(-> (n) over barK(S)(0)).
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10. |
- Ablikim, M., et al.
(författare)
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Measurement of e(+)e(-) -> D(D)over-bar cross sections at the psi(3770) resonance
- 2018
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Ingår i: Chinese Physics C. - : IOP PUBLISHING LTD. - 1674-1137 .- 2058-6132. ; 42:8
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Tidskriftsartikel (refereegranskat)abstract
- We report new measurements of the cross sections for the production of D (D) over bar final states at the psi(3770) resonance. Our data sample consists of an integrated luminosity of 2.93 fb(-1) of e(+)e(-) annihilation data produced by the BEPCII collider and collected and analyzed with the BESIII detector. We exclusively reconstruct three D-0 and six D+ hadronic decay modes and use the ratio of the yield of fully reconstructed D (D) over bar events ("double tags") to the yield of all reconstructed D or (D) over bar mesons ("single tags") to determine the number of D-0(D) over bar (0) and D+D- events, benefiting from the cancellation of many systematic uncertainties. Combining these yields with an independent determination of the integrated luminosity of the data sample, we find the cross sections to be sigma(e(+)e(-) -> D-0(D) over bar (0)(-) )=(3.615 +/- 0.010 +/- 0.038) nb and sigma(e(+)e(-) -> D+D-)=(2.830 +/- 0.011 +/- 0.026) nb, where the uncertainties are statistical and systematic, respectively.
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11. |
- Ablikim, M., et al.
(författare)
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Measurement of higher-order multipole amplitudes in psi(3686) -> gamma chi(c1,2) with chi(c1,2) -> gamma J/psi and search for the transition eta(c)(2S) -> gamma J/psi
- 2017
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Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 95:7
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Tidskriftsartikel (refereegranskat)abstract
- Using 106 x 10(6) psi(3686) events collected with the BESIII detector, we measure multipole amplitudes for the decay psi(3686) ->; gamma chi(c1,2) -> gamma gamma J/psi beyond the dominant electric-dipole amplitudes. The normalized magnetic-quadrupole (M2) amplitude for psi(3686) -> gamma chi(c1,2) -> gamma gamma J/psi and the normalized electric-dipole amplitudes for psi(3686) -> gamma chi(c2) -> gamma J/psi and determined. The M2 amplitudes for psi(3686) -> gamma chi(c1) and ; chi(c1,2) -> gamma J/psi are found to differ significantly from zero and are consistent with theoretical predictions. We also obtain the ratios of M2 contributions of psi(3686) and J/psi decays to;2,chi(c1,2,) b(2)(1/)b(2)(2) = 1.35 +/- 0.72 and a(2)(1/)a(2)(2) = 0.617 +/- 0.083,,which agree well with theoretical expectations. By considering the multipole contributions of chi(c1,2), we measure the product branching fractions for the cascade decays psi(3686) -> gamma chi(c 0,1,2) -> gamma gamma J/psi and search for the process eta(c)(2s) -> gamma J/psi through psi(3686) -> gamma eta(c)(2s).The product branching fraction for psi(3686) -> gamma chi(c0) -> gamma gamma J/psi is 3 sigma larger than published measurements, while those of psi(3686) -> gamma chi(c1,2) -> gamma gamma J/psi are consistent. No significant signal for the decay psi(3686) -> gamma eta(c) (2s) -> gamma gamma J/psi is observed, and the upper limit of the product branching fraction at the 90% confidence level is determined.
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12. |
- Ablikim, M., et al.
(författare)
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Measurement of the integrated Luminosities of cross-section scan data samples around the psi(3770) mass region
- 2018
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Ingår i: Chinese Physics C. - : SCIENCE PRESS. - 1674-1137 .- 2058-6132. ; 42:6
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Tidskriftsartikel (refereegranskat)abstract
- To investigate the nature of the psi(3770) resonance and to measure the cross section for e(+)e(-) -> D (D) over bar, a cross-section scan data sample, distributed among 41 center-of-mass energy points from 3.73 to 3.89 GeV, was taken with the BESIII detector operated at the BEPCII collider in the year 2010. By analyzing the large angle Bhabha scattering events, we measure the integrated luminosity of the data sample at each center-of-mass energy point. The total integrated luminosity of the data sample is 76.16 +/- 0.04 +/- 0.61 pb(-1), where the first uncertainty is statistical and the second systematic.
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13. |
- Ablikim, M., et al.
(författare)
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Observation of a Neutral Structure near the D(D)over-bar* Mass Threshold in e(+)e(-) -> (D(D)over-bar*)(0)pi(0) at root s=4.226 and 4.257 GeV
- 2015
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Ingår i: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 115:22
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Tidskriftsartikel (refereegranskat)abstract
- A neutral structure in the D (D) over bar* system around the D (D) over bar* mass threshold is observed with a statistical significance greater than 10 sigma in the processes e(+)e(-) -> D+D*(-)pi(0) + c.c. and e(+)e(-) -> D-0(D) over bar*(0)pi(0) + c.c. at root s = 4.226 and 4.257 GeV in the BESIII experiment. The structure is denoted as Z(c)(3885)(0). Assuming the presence of a resonance, its pole mass and width are determined to be [3885.7(-5.7)(+4.3) (stat) +/- 8.4(syst)] MeV/c(2) and [35(-12)(+11) (stat) +/- 15(syst)] MeV, respectively. The Born cross sections are measured to be sigma[e(+)e(-) -> Z(c)(3885)(0)pi(0); Z(c)(3885)(0) -> D (D) over bar*] = [77 +/- 13(stat) +/- 17(syst)] pb at 4.226 GeV and [47 +/- 9(stat) +/- 10(syst)] pb at 4.257 GeV. The ratio of decay rates B[Z(c)(3885)(0) -> D+D*(-) + c.c.]/B[Z(c)(3885)(0) -> D-0(D) over bar*(0) + c.c.] is determined to be 0.96 +/- 0.18(stat) +/- 0.12(syst), consistent with no isospin violation in the process, Z(c)(3885)(0) -> D (D) over bar*.
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14. |
- Ablikim, M., et al.
(författare)
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Observation of psi(3686) -> eta ' e(+)e(-)
- 2018
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Ingår i: Physics Letters B. - : ELSEVIER SCIENCE BV. - 0370-2693 .- 1873-2445. ; 783, s. 452-458
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Tidskriftsartikel (refereegranskat)abstract
- Using a data sample of 448.1 x 10(6) psi(3686) events collected with the BESIII detector at the BEPCII collider, we report the first observation of the electromagnetic Dalitz decay psi(3686) -> eta'e(+)e(-), with significances of 7.0 sigma and 6.3 sigma when reconstructing the eta' meson via its decay modes eta' -> gamma pi(+)pi(-) and eta' -> pi(+)pi(-) eta (eta -> gamma gamma), respectively. The weighted average branching fraction is determined to be B(psi(3686) -> eta'e(+)e(-)) = (1.90 +/- 0.25 +/- 0.11) x 10(-6), where the first uncertainty is statistical and the second systematic.
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15. |
- Ablikim, M., et al.
(författare)
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Observation of the Semileptonic Decay D-0 -> a(0)(980)(-)e(+)nu(e) and Evidence for D+ -> a(0)(980)(0)e(+)nu(e)
- 2018
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Ingår i: Physical Review Letters. - : AMER PHYSICAL SOC. - 0031-9007 .- 1079-7114. ; 121:8
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Tidskriftsartikel (refereegranskat)abstract
- Using an e(+)e(-) collision data sample of 2.93 fb(-1) collected at a center-of-mass energy of 3.773 GeV by the BESIII detector at BEPCII, we report the observation of D-0 -> a(0)(980)(-)e(+)nu(e) and evidence for D+ -> a(0)(980)(0)e(+)nu(e) with significances of 6.4 sigma and 2.9 sigma, respectively. The absolute branching fractions are determined to be B(D-0 -> a(0)(980)(-)e(+)nu(e)) x B(a(0)(980)(-) -> eta pi(-)) = [1.33(-0.29)(+0.33)(stat) +/- 0.09(syst)] x 10(-4) and B(D+ -> a(0)(980)(0)e(+)nu(e)) x B(a(0)(980)(0) -> eta pi(0)) = [1.66(-0.66)(+0.81)(stat) +/- 0.11(syst) x 10(-4). This is the first time the a(0)(980) meson has been measured in a D-0 semileptonic decay, which would open one more interesting page in the investigation of the nature of the puzzling a(0)(980) states.
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16. |
- Ablikim, M., et al.
(författare)
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Observation of the Singly Cabibbo-Suppressed Decay D+ -> omega pi(+) and Evidence for D-0 -> omega pi(0)
- 2016
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Ingår i: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 116:8
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Tidskriftsartikel (refereegranskat)abstract
- Based on 2.93 fb(-1) e(+)e(-) collision data taken at center-of-mass energy of 3.773 GeV by the BESIII detector, we report searches for the singly Cabibbo-suppressed decays D+ -> omega pi(+) and D-0 -> omega pi(0). A double tag technique is used to measure the absolute branching fractions B(D+ -> omega pi(+)) = (2.79 +/- 0.57 +/- 0.16) x 10(-4) and B(D-0 +/- omega pi(0)) = (1.17 +/- 0.34 +/- 0.07) x 10(-4), with statistical significances of 5.5 sigma and 4.1 sigma, where the first and second uncertainties are statistical and systematic, respectively.
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17. |
- Ablikim, M., et al.
(författare)
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Precision Measurement of the e(+)e(-) -> Lambda(+)(c)(Lambda)over-bar(c)(-) Cross Section Near Threshold
- 2018
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Ingår i: Physical Review Letters. - : American Physical Society. - 0031-9007 .- 1079-7114. ; 120:13
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Tidskriftsartikel (refereegranskat)abstract
- The cross section of the e(+)e(-) -> Lambda(+)(c)(Lambda) over bar (-)(c) process is measured with unprecedented precision using data collected with the BESIII detector at root s = 4574.5, 4580.0, 4590.0 and 4599.5 MeV. The nonzero cross section near the Lambda(+)(c)(Lambda) over bar (-)(c) production threshold is cleared. At center-of-mass energies root s = 4574.5 and 4599.5 MeV, the higher statistics data enable us to measure the Lambda(c) polar angle distributions. From these, the.c electric over magnetic form-factor ratios (vertical bar G(E)/G(M)vertical bar) are measured for the first time. They are found to be 1.14 +/- 0.14 +/- 0.07 and 1.23 +/- 0.05 +/- 0.03, respectively, where the first uncertainties are statistical and the second are systematic.
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18. |
- Ablikim, M., et al.
(författare)
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Search for invisible decays of omega and phi with J/psi data at BESIII
- 2018
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Ingår i: Physical Review D. - : American Physical Society. - 2470-0010 .- 2470-0029. ; 98:3
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Tidskriftsartikel (refereegranskat)abstract
- Using a data sample of (1310.6 +/- 7.0) x 10(6) J/psi events collected with the BESIII detector operating at the BEPCII collider, we perform the first experimental search for invisible decays of a light vector meson (V = omega, phi) via J/psi -> V-eta decays. The decay of eta -> pi(+)pi(-)pi(0) is utilized to tag the V meson decaying into the invisible final state. No evidence for a significant invisible signal is observed, and the upper limits on the ratio of branching fractions at the 90% confidence level are determined to be B(omega -> invisible)/B(omega -> pi(+)pi(-)pi(0)) < 8.1 x 10(-5) and B(phi -> invisible)/B(phi -> K+K-) < 3.4 x 10(-4). By using the world average values of B(omega -> pi(+)pi(-)pi(0) and B(phi -> K+K-,) the upper limits on the decay branching fractions at the 90% confidence level are set as B(omega -> invisible) < 7.3 x 10(-5) and B(phi -> invisible) < 1.7 x 10(-4), respectively.
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19. |
- Ablikim, M., et al.
(författare)
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Search for the rare decay of ψ(3686)→Λ+c¯pe+e−+c.c. at BESIII
- 2018
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Ingår i: Physical Review D. - 2470-0010 .- 2470-0029. ; 97:9
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Tidskriftsartikel (refereegranskat)abstract
- Based on a data sample of (448.1 +/- 2.9) x 10(6)Psi(3686) decays collected with the BESIII experiment, a search for the flavor changing neutral current transition Psi(3686) -> Lambda(+)(c) pe(+) e(-) + c.c. is performed for the first time. No signal candidates are observed and the upper limit on the branching fraction of Psi(3686) -> Lambda(+)(c) pe(+) e(-) is determined to be 1.7 x 10(-6) at the 90% confidence level. The result is consistent with expectations from the standard model, and no evidence for new physics is found.
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20. |
- Ablikim, M., et al.
(författare)
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Search for the rare decays D -> h(h((')))e(+) e(-)
- 2018
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Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 97:7
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Tidskriftsartikel (refereegranskat)abstract
- We search for rare decays of D mesons to hadrons accompanied by an electron-positron pair (h(h((')))e(+)e(- )),using an e(+)e(-) collision sample corresponding to an integrated luminosity of 2.93 fb(-1) collected with the BESIII detector at root s = 3.773 GeV. No significant signals are observed, and the corresponding upper limits on the branching fractions at the 90% confidence level are determined. The sensitivities of the results are at the level of 10(-5)-10(-6), providing a large improvement over previous searches.
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21. |
- Ablikim, M., et al.
(författare)
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Study of D+ -> K-pi(+)e(+)nu(e)
- 2016
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Ingår i: PHYSICAL REVIEW D. - 2470-0010. ; 94:3
-
Tidskriftsartikel (refereegranskat)abstract
- We present an analysis of the decay D+ -> K-pi(+)e(+)nu(e) based on data collected by the BESIII experiment at the psi(3770) resonance. Using a nearly background-free sample of 18262 events, we measure the branching fraction B(D+ -> K-pi+e+nu e) = (3.77 +/- 0.03 +/- 0.08)%. For 0.8 < m(K pi) < 1.0 GeV/c(2), the partial branching fraction is B(D+ -> K-pi+e+nu e)([0.8,1.0]) = (3.39 +/- 0.03 +/- 0.08)%. A partial wave analysis shows that the dominant (K) over bar* (892)degrees component is accompanied by an S-wave contribution accounting for (6.05 +/- 0.22 +/- 0.18)% of the total rate and that other components are negligible. The parameters of the (K) over bar* (892)degrees resonance and of the form factors based on the spectroscopic pole dominance predictions are also measured. We also present a measurement of the (K) over bar* (892)degrees helicity basis form factors in a model-independent way.
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22. |
- Ablikim, M., et al.
(författare)
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Study of decay dynamics and CP asymmetry in D+ -> K(L)(0)e(+)nu(e) decay
- 2015
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Ingår i: Physical Review D. - 1550-7998 .- 1550-2368. ; 92:11
-
Tidskriftsartikel (refereegranskat)abstract
- Using 2.92 fb(-1) of electron-positron annihilation data collected at root s = 3.773 GeV with the BESIII detector, we obtain the first measurements of the absolute branching fraction B(D+ -> K(L)(0)e(+)nu(e)) = (4.481 +/- 0.027(stat) +/- 0.103(sys))% and the CP asymmetry A(CP)(D+-> KL0e+nu e) = (-0.59 +/- 0.60(stat) +/- 1.48(sys))%. From the D+ -> K(L)(0)e(+)nu(e) differential decay rate distribution, the product of the hadronic form factor and the magnitude of the Cabibbo-Kobayashi-Maskawa matrix element, f(+)(K)(0)vertical bar V-cs vertical bar, is determined to be 0.728 +/- 0.006(stat) +/- 0.011(sys). Using vertical bar V-cs vertical bar from the SM constrained fit with the measured f(+)(K)(0)vertical bar V-cs vertical bar, f(+)(K)(0) = 0.748 +/- 0.007(stat) +/- 0.012(sys) is obtained, and utilizing the unquenched Lattice QCD (LQCD) calculation for f(+)(K)(0), vertical bar V-cs vertical bar = 0.975 +/- 0.008(stat) +/- 0.015(sys) +/- 0.025(LQCD).
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