| 1. |
- Awasthi, Mukesh Kumar, et al.
(författare)
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Filamentous fungi for sustainable vegan food production systems within a circular economy : Present status and future prospects
- 2023
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Ingår i: Food Research International. - : Elsevier BV. - 0963-9969 .- 1873-7145. ; 164
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Tidskriftsartikel (refereegranskat)abstract
- Filamentous fungi serve as potential candidates in the production of different value-added products. In the context of food, there are several advantages of using filamentous fungi for food. Among the main advantages is that the fungal biomass used food not only meets basic nutritional requirements but that it is also rich in protein, low in fat, and free of cholesterol. This speaks to the potential of filamentous fungi in the production of food that can substitute animal-derived protein sources such as meat. Moreover, life-cycle analyses and techno-economic analyses reveal that fungal proteins perform better than animal-derived proteins in terms of land use efficiency as well as global warming. The present article provides an overview of the potential of filamentous fungi as a source of food and food supplements. The commercialization potential as well as social, legal and safety issues of fungi-based food products are discussed.
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| 2. |
- Braho, Vjola, et al.
(författare)
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Cultivation of edible filamentous fungi on pomegranate by-products as feedstocks to produce mycoprotein
- 2023
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Ingår i: Systems Microbiology and Biomanufacturing. - : Springer. - 2662-7655 .- 2662-7663.
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Tidskriftsartikel (refereegranskat)abstract
- Pomegranate, renowned for its delectable taste and remarkable nutritional profile, has witnessed a surge in both production and consumption. However, the by-products generated during industrial processes, such as peels and seeds, have the potential for adverse environmental impacts if not meticulously managed. Similarly, expired fruit juices or spillages that may occur during manufacturing and transportation contribute to agri-food waste. This study focused on the comprehensive assessment of pomegranate by-products and pomegranate juice using ascomycetes and zygomycetes filamentous fungi, namely Aspergillus oryzae, Rhizopus oligosporus, and Neurospora intermedia to obtain mycoprotein for sustainable vegan food production. The findings revealed that pomegranate juice, both fresh and expired commercial, contained essential nutrients for fungal biomass production (up to 0.024 g biomass/mL juice). Nonetheless, fresh juice emerges as a more potent medium in terms of protein production than commercial juice. Cultivating A. oryzae yielded a biomass of 0.39 (g biomass/g peel) from pomegranate peel, while concurrently raising the protein content of raw pomegranate peel from 30.89 g/kg to 85.41 g/kg. Furthermore, incorporating yeast extract into the peel medium not only resulted in an enhanced biomass yield of 0.49 (g biomass/g peel) but also significantly elevated the protein content to 198.63 g/kg. This study provides valuable insights into the potential of pomegranate peel and juice as promising substrate for fungal biomass production, offering opportunities for the development of innovative food and feed products.
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| 3. |
- Celik, Kamile Burcu, et al.
(författare)
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In-situ wrapping of tin oxide nanoparticles by bacterial cellulose derived carbon nanofibers and its application as freestanding interlayer in lithium sulfide based lithium-sulfur batteries
- 2018
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Ingår i: Journal of Colloid and Interface Science. - : Elsevier BV. - 0021-9797 .- 1095-7103. ; 530, s. 137-145
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Tidskriftsartikel (refereegranskat)abstract
- Lithium-Sulfur (Li-S) batteries are mostly known for their high energy density and cost-effectiveness. However, their intrinsic problems hinder their implementation into the marketplace. The most pronounced problems are the parasitic reactions which occur between lithium polysulfides species and lithium metal anode, the volume expansion of sulfur (80%) at the end of discharge and the safety issues which are linked with the use of lithium metal. Herein this work, two approaches are applied to prevent these effects; one approach is the use of Li2S as cathode material, instead of starting from sulfur powder, both to circumvent the volume expansion of sulfur taking place during discharge and to enable lithium-free anodes cell assembling (i.e. Si-Li2S or Sn-Li2S cell configurations). Second approach deals with the lithium anode protection by SnO2 containing freestanding pyrolyzed bacterial cellulose interlayers located between anode and cathode electrodes. Since bacterial celluloses are formed in the presence of SnO2 nanoparticles, the resulting structure enables intimate contact between carbon and SnO2 nanoparticles. By employing Li2S cathode and freestanding interlayer concurrently, 468 mAh g−1 discharge capacity is obtained at C/10 current density over 100 cycles.
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| 4. |
- Ding, Zheli, et al.
(författare)
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Agro waste as a potential carbon feedstock for poly-3-hydroxy alkanoates production: Commercialization potential and technical hurdles
- 2022
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Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 364
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Tidskriftsartikel (refereegranskat)abstract
- The enormous production and widespread applications of non -biodegradable plastics lead to their accumulation and toxicity to animals and humans. The issue can be addressed by the development of eco-friendly strategies for the production of biopolymers by utilization of waste residues like agro residues. This will address two societal issues – waste management and the development of an eco-friendly biopolymer, poly-3-hydroxy alkanoates (PHAs). Strategies adopted for utilization of agro-residues, challenges and future perspectives are discussed in detail in this comprehensive review. The possibility of PHA properties improvements can be increased by preparation of blends.
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| 5. |
- Ding, Zheli, et al.
(författare)
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Valorization of tropical fruits waste for production of commercial biorefinery products : A review
- 2023
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Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 374
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Forskningsöversikt (refereegranskat)abstract
- Tropical fruit wastes (TFW) are considered as the major source of food and nutrition in the topical countries. In the recent years, modernization of agriculture has increased the tropical fruit production. Higher fruit production led to an increasing abundance in the tropical fruit waste. In general, the tropical fruit waste has no economic value and ends up in landfill. But in recent years it was observed that the tropical fruit waste can be valorized to produce value-added products ranging from compost, phytochemicals, and food products to biofuels. The tropical fruit waste has great potential to produce useful products in tropical areas. This review literature is an endeavor to understand the major tropical fruit wastes and their composition. The review presents a detailed investigation on tropical fruit waste composition, its conversion potential, role of microbes in waste valorization, production of commercially valuable products and future perspectives in waste valorization.
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| 6. |
- Harirchi, Sharareh, et al.
(författare)
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Bacillales: From Taxonomy to Biotechnological and Industrial Perspectives
- 2022
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Ingår i: Microorganisms. - : MDPI AG. - 2076-2607. ; 10:12
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Tidskriftsartikel (refereegranskat)abstract
- For a long time, the genus Bacillus has been known and considered among the most applicable genera in several fields. Recent taxonomical developments resulted in the identification of more species in Bacillus-related genera, particularly in the order Bacillales (earlier heterotypic synonym: Caryophanales), with potential application for biotechnological and industrial purposes such as biofuels, bioactive agents, biopolymers, and enzymes. Therefore, a thorough understanding of the taxonomy, growth requirements and physiology, genomics, and metabolic pathways in the highly diverse bacterial order, Bacillales, will facilitate a more robust designing and sustainable production of strain lines relevant to a circular economy. This paper is focused principally on less-known genera and their potential in the order Bacillales for promising applications in the industry and addresses the taxonomical complexities of this order. Moreover, it emphasizes the biotechnological usage of some engineered strains of the order Bacillales. The elucidation of novel taxa, their metabolic pathways, and growth conditions would make it possible to drive industrial processes toward an upgraded functionality based on the microbial nature.
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| 7. |
- Haykir, N. I., et al.
(författare)
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Applications of ionic liquids for the biochemical transformation of lignocellulosic biomass into biofuels and biochemicals : A critical review
- 2023
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Ingår i: Biochemical engineering journal. - : Elsevier B.V.. - 1369-703X .- 1873-295X. ; 193
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Forskningsöversikt (refereegranskat)abstract
- Lignocellulosic materials are valuable resources in today's bioprocess technologies; however, their recalcitrance is a major barrier in industry regarding their conversion to microbial products. For this purpose, in this study, the synthesis of ionic liquids (ILs), its function in the hydrolysis of lignocellulosic materials, its biochemistry and possible toxic effects were investigated. In addition, the bioconversion of lignocellulosic materials pretreated with ionic liquids to biofuels (bioethanol, biobutanol, biogas and hydrogen) and various biochemicals is discussed in detail. For this, the focus is on the potential of ILs for industrial integration and use in large-scale reactors. ILs offer significant advantages due to their potential for ease of use and their features such as recovery and reuse after pretreatment. However, there are economic and technical problems that need to be solved to expand ILs in industrial systems and increase their use potential. To overcome these problems and the usability of ILs technologies in industry, techno-economic analyses has been examined and compared with traditional processes.
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| 8. |
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| 9. |
- Mukesh Kumar, Awasthi, et al.
(författare)
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A comprehensive review on thermochemical, and biochemical conversion methods of lignocellulosic biomass into valuable end product
- 2023
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Ingår i: Fuel. - : Elsevier. - 0016-2361 .- 1873-7153. ; 342
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Tidskriftsartikel (refereegranskat)abstract
- Lignocellulosic wastes have emerged as a potential feedstock in the last decades. There are multiple reasons for its abundance, easy availability, economic, and abundant sources. It can be used to produce several value-added products. Among them, fuel is considered one of the important requirements. Production of fuel from lignocellulosic biomass is a tricky business. The major reason for its failure is the low product yield. Therefore, high yield and low-cost are the two key parameters which need significant optimization. To achieve the target several newer technologies such as pyrolysis, hydrothermal liquefaction and gasification have emerged. These techniques are much more efficient than that of conventional acid or alkali. At the same time quality of the product is also improved. The focus of this review is to analyze the efficiency of chemical conversion of lignocellulosic residues into valuable fuels keeping in mind the cost-reduction strategies.
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| 10. |
- Mukesh Kumar, Awasthi, et al.
(författare)
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Myco-biorefinery approaches for food waste valorization : Present status and future prospects
- 2022
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Ingår i: Bioresource Technology. - : Elsevier. - 0960-8524 .- 1873-2976. ; 360
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Tidskriftsartikel (refereegranskat)abstract
- Increases in population and urbanization leads to generation of a large amount of food waste (FW) and its effective waste management is a major concern. But putrescible nature and high moisture content is a major limiting factor for cost effective FW valorization. Bioconversion of FW for the production of value added products is an eco-friendly and economically viable strategy for addressing these issues. Targeting on production of multiple products will solve these issues to greater extent. This article provides an overview of bioconversion of FW to different value added products.
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| 11. |
- Nazir, Muhammad Tahir, et al.
(författare)
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Production of filamentous fungal biomass with increased oil content using olive oil as a carbon source
- 2022
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Ingår i: Journal of chemical technology and biotechnology (1986). - : John Wiley & Sons. - 0268-2575 .- 1097-4660.
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUNDVegetable oil is more difficult for microorganisms to degrade compared with carbohydrates and protein. Oil-rich waste creates serious environmental and health concerns if it remains untreated. The aim of the work reported here was to evaluate the effects on the nutritional composition of filamentous fungal biomass by growing it in pure olive oil as a carbon source.RESULTSThe growth of different filamentous fungal strains (Aspergillus oryzae, Neurospora intermedia and Rhizopus oryzae) was investigated in pure olive oil. A pre-germination stage of either glucose or yeast extract was necessary for successful fungal growth in olive oil. A. oryzae showed superior performance in comparison with N. intermedia and R. oryzae in terms of biomass growth. The obtained biomass from A. oryzae and N. intermedia was analyzed for protein, fat, ash and alkali-insoluble material, where the presence of olive oil had a steering effect on biomass growth (16 g L−1 with oil versus 4 g L−1 without oil). Nutritional composition of the fungal biomass of A. oryzae contained 0.33% fat and 48% protein when cultivated in medium without olive oil supplementation, while 31% fat and 14% protein contents were observed in the presence of olive oil-containing medium. Similar trends for fat and protein contents were observed for the biomass of N. intermedia. Moreover, microscopy confirmed the presence of oil globules inside the fungal cells.CONCLUSIONSFat composition of fungal biomass can be steered through addition of olive oil, which increases the versatility of the produced biomass for various applications, namely in feed, food and biofuel production.
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| 12. |
- Rousta, Neda, et al.
(författare)
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Effects of fungal based bioactive compounds on human health: Review paper
- 2023
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Ingår i: Critical reviews in food science and nutrition. - : Informa UK Limited. - 1040-8398 .- 1549-7852. ; , s. 1-24
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Forskningsöversikt (refereegranskat)abstract
- Since the first years of history, microbial fermentation products such as bread, wine, yogurt and vinegar have always been noteworthy regarding their nutritional and health effects. Similarly, mushrooms have been a valuable food product in point of both nutrition and medicine due to their rich chemical components. Alternatively, filamentous fungi, which can be easier to produce, play an active role in the synthesis of some bioactive compounds, which are also important for health, as well as being rich in protein content. Therefore, this review presents some important bioactive compounds (bioactive peptides, chitin/chitosan, β-glucan, gamma-aminobutyric acid, L-carnitine, ergosterol and fructooligosaccharides) synthesized by fungal strains and their health benefits. In addition, potential probiotic- and prebiotic fungi were researched to determine their effects on gut microbiota. The current uses of fungal based bioactive compounds for cancer treatment were also discussed. The use of fungal strains in the food industry, especially to develop innovative food production, has been seen as promising microorganisms in obtaining healthy and nutritious food.
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| 13. |
- Sar, Taner, Postdoctor, 1989-, et al.
(författare)
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A study on the use of olive oil mill wastewater to produce protein-rich fungal biomass
- 2024
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Konferensbidrag (refereegranskat)abstract
- While olive oil is an important food product in the Mediterranean Sea Basin, olive oil mill wastewater (OOMW) and olive pomace, which are by-products of olive oil, are released in excessive quantities. OMWW is an important source of environmental pollutants due to its slightly acidic pH, high contents of phenol and chemical oxygen demand (COD). To overcome this problem, it was aimed to investigate the potential use of OOMW as an alternative substrate for biomass production by filamentous fungi in this study. For the cultivation, three edible fungi (Aspergillus oryzae CBS 819.72, Neurospora intermedia CBS 131.92, and Rhizopus delemar CBS 145940) were tested. Among them, A. oryzae was found to be a promising fungus in biomass production containing 14.9% protein. The protein content of the biomass was improved to 44.9% (w/w) by adding a nitrogen source (sodium nitrate) and removing the suspended solids. Concomitantly, 35-44% of COD reduction was also obtained after the fungal cultivation. Thus, the potential use of olive oil mill wastewater for the cultivation of fungal biomass was determined and at the same time, its pre-treatment was provided. However, the content of the obtained fungal biomass should be determined and its usability as feed should be investigated.
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| 14. |
- Sar, Taner, Postdoctor, 1989-, et al.
(författare)
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Antimicrobial Activities of Olive Oil Mill Wastewater Extracts against Selected Microorganisms
- 2023
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Ingår i: Sustainability. - 2071-1050. ; 15:10
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Tidskriftsartikel (refereegranskat)abstract
- Discovering eco-friendly alternatives to synthetic chemicals has become an increasingly popular area of research. Natural products are now in the spotlight for their potential use as replacements for synthetic chemicals. To maximize the benefits of these natural products, it is important to use efficient extraction methods, especially from agroindustrial waste. Olive oil mill wastewater (OOMW) is a byproduct of the olive oil production process and is considered a pollutant; however, OOMW contains a wide range of phenolic compounds that have proven antimicrobial properties. This study investigates the extraction of these compounds from OOMW, with the aim of determining their potential antimicrobial activities against several bacterial strains and fungi, including Bacillus spizizenii, Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella aerogenes, Streptococcus uberis, Enterococcus faecalis, and Candida albicans. The OOMW extracts (OEs) were prepared by using three different solvents: ethyl acetate, ethanol, and methanol. The highest total phenolic contents (4.03 g, GAE/L) and the strongest antibacterial activity were obtained with methanol extraction. All OEs showed no antifungal activity against C. albicans. OEs, particularly methanol extracts of OOMW, can be used as bioactive substances in various industries as nutraceuticals and food ingredients, respectively.
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| 15. |
- Sar, Taner, Postdoctor, 1989-, et al.
(författare)
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Biorecovering of phenolic-rich compounds from food industry wastes
- 2022
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Konferensbidrag (refereegranskat)abstract
- In food industry, abundant fruit/vegetable byproducts (peel, seed, etc.) have been generated and released into the environment. These wastes can be considered as a largely available, low-cost source of value-added compounds. Among these, phenolic compounds are well-known for their beneficial effects on human health. Effective extraction methodologies are required for recovery of these phenolic compounds from agri-food wastes. These extracts can be used used as natural antimicrobials in pharmacology or disinfectants in food processing plants. In this work, pomegranate peel extracts were prepared by using different solvents to obtain high amount of phenolic-rich compounds. The pomegranate peel extracts were also tested against some important food pathogens to determine their antimicrobial and antibiofilm activities. Pomegranate peel extracts having high amount of phenolic compounds had higher antimicrobial and antibiofilm activities. As a result, phenolic-rich bioactive compounds can be recovered from food industry wastes and used as natural antimicrobial and antibiofilm agents. Thus, food industry wastes, especially fruit wastes, can be integrated into the extraction process and use in food, pharmacology, cosmetic and medicine industries.
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| 16. |
- Sar, Taner, Postdoctor, 1989-, et al.
(författare)
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Evaluation of the Vegetation Period According to Climate Change Scenarios: A Case Study in the Inner West Anatolia Subregion of Turkey*
- 2019
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Ingår i: Journal of Geography. - : Istanbul University. - 1302-7212 .- 1305-2128. ; , s. 29-39
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Tidskriftsartikel (refereegranskat)abstract
- In this research, 15 meteorological stations located in western Anatolia in Turkey were investigated for determining temperature properties. The vegetation season has been determined according to days when the daily temperature was greater than or equal to 8°C. The research area has 3 different vegetation durations as longer (more than 225 days in the western and southern parts), moderate (210-220 days in plateaus around Uşak, Gediz and Demirci), and shorter (180-195 days in the eastern part and less than 180 days in mountainous areas). Then, the vegetation has been reinvestigated according to climate change scenarios (RCP 4.5 and 8.5). For this purpose, the daily mean temperatures were raised by 2.6°C and 4.8°C for RCP 4.5 and RCP 8.5, respectively. In the reinvestigation period, the vegetation period will increase about 15-20 days or over 40 days according to RCP 4.5 and 8.5, respectively. Along with the increase in temperature throughout the research area, growing seasons will be significantly affected, beginning and ending dates of vegetation would shift. Owing to climate change effects, mountainous areas may be affected more than low-altitude areas. As a conclusion due to the result of increasing temperature, plant development and distribution will be affected, and new plant species may occur
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| 17. |
- Sar, Taner, Postdoctor, 1989-, et al.
(författare)
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Evaluation of fish industry byproducts for protein-rich fungal biomass production
- 2021
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Konferensbidrag (refereegranskat)abstract
- In connection with the increasing population in the world, the food industry has been developing rapidly and the industrial by-products have become an important problem. Fish industry byproducts are rich in minerals, nitrogen, and fat, and can contribute to environmental pollution if not properly treated. These byproducts are generally evaluated for biogas production and/or follow wastewater treatment. The byproducts can alternatively be utilized for the cultivation of microorganisms and converted to value-added products. For this purpose, two edible filamentous fungi, namely Aspergillus oryzae and Rhizopus oryzae were used to evaluate the production of protein-rich fungal biomass from fish processing byproducts. The amount of fungal biomass and its protein content (35-65%) varied according to the origin of the byproduct within the fish filleting process. Along with the fungal growth and production of a protein-rich product, nitrogen and COD removal of 32–72% and 12–58%, respectively, were achieved. Altogether, cultivation of filamentous fungi can aid to divert fish processing byproducts from biogas production towards production of protein-rich products for animal feed and concomitantly ease wastewater treatment.
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| 18. |
- Sar, Taner, Postdoctor, 1989-, et al.
(författare)
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Improvement in desulfurization of dibenzothiophene and dibenzothiophene sulfone by Paenibacillus strains using immobilization or nanoparticle coating
- 2022
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Ingår i: Journal of Applied Microbiology. - : Oxford University Press (OUP). - 1364-5072 .- 1365-2672. ; 133:2, s. 1040-1051
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Tidskriftsartikel (refereegranskat)abstract
- AimsBiodesulfurization of fossil fuels is a promising technology for deep desulfurization. Previously, we have shown that Paenibacillus strains 32O-W and 32O-Y can desulfurize dibenzothiophene (DBT) and DBT sulfone (DBTS) effectively. In this work, improvements in DBT and DBTS desulfurization by these strains were investigated through immobilization and nanoparticle coating of cells.Methods and ResultsPaenibacillus strains 32O-W and 32O-Y immobilized in alginate gel beads or coated with Fe3O4 magnetite nanoparticles were grown at various concentrations (0.1–2 mmol l−1) of DBT or DBTS for 96 h. The production of 2-hydroxybiphenyl (2-HBP) from the 4S pathway biotransformation of DBT or DBTS was measured. The highest amounts of 2-HBP production occurred at concentrations of 0.1 and 0.5 mmol l−1. Compared to planktonic cultures maximum 2-HBP production increased by 54% for DBT and 90% for DBTS desulfurization with immobilized strains, and 44% for DBT and 66% for DBTS desulfurization by nanoparticle-coated strains.ConclusionsNanoparticle-coated and immobilized cells may be of use in efforts to increase the efficiency of biodesulfurization.Significance and Impact of the StudyAlginate immobilization or nanoparticle coating of bacterial cells may be useful approaches for the enhancement of biodesulfurization for eventual use on an industrial scale.
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| 19. |
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| 20. |
- Sar, Taner, Postdoctor, 1989-, et al.
(författare)
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Integration of olive oil industry wastewaters into biorefinery
- 2023
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Konferensbidrag (refereegranskat)abstract
- While olive oil is an important food product in the Mediterranean Sea Basin, olive oil mill wastewater (OOMW) and olive pomace, which are by-products of olive oil, are released in excessive quantities. OMWW is an important source of environmental pollutants due to its slightly acidic pH, high phenol content and high chemical oxygen demand (COD). To overcome this problem, it was aimed to investigate the potential use of OOMW as an alternative substrate for protein rich biomass and bacterial cellulose production by filamentous fungi and acetic acid bacterium, respectively, in this study. Additionally, extracts of OOMW against selected microorganisms were examined to determine their use for industrial applications. The finding results indicated that OOMW needs nitrogen supplementation for microbial production processes. On the other hand, extracts of OOMW showed significant antibacterial activities with high phenolic contents. Overall, OOMW can be integrated into biorefinery with both microbial production and extraction processes and the resulting products will contribute to the food and cosmetic industries.
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| 21. |
- Sar, Taner, Postdoctor, 1989-, et al.
(författare)
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Media preparation and sterilization in bioprocesses
- 2022
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Ingår i: Advances in Bioprocess Engineering. - : Elsevier. - 9780323911672 - 9780323984836 ; , s. 399-430
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Fermentation and bioprocesses in which various metabolites from food to pharmaceutics are produced are constantly evolving. This section is devoted to contamination risks and their eliminations, media preparation, decontamination, and sterilization methods for such processes. For contamination, sources of microbial contaminations and decontamination (physical and chemical) practices are discussed. In addition, sterilization methods (heat, filtration, chemical, and radiation) that are applied for the removal of microorganisms from small-scale to large-scale instruments, gases, and liquids are explained. In the preparation of the media, the batching area, developed equipment, and methods for large-scale reactors are mentioned. In summary, the preparation and sterilization of the media in bioprocesses from laboratory-scale reactors to industrial-scale reactors are evaluated.
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| 22. |
- Sar, Taner, Postdoctor, 1989-, et al.
(författare)
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Novel Perspectives on Food-Based Natural Antimicrobials: A Review of Recent Findings Published since 2020
- 2023
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Ingår i: Microorganisms. - 2076-2607. ; 11:9
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Forskningsöversikt (refereegranskat)abstract
- Various fruit and vegetable wastes, particularly peels, seeds, pulp, and unprocessed residues from the food industry, are abundant sources of antioxidants and essential antimicrobial agents. These valuable bioactive compounds recovered from the food industry have a great application in food, agriculture, medicine, and pharmacology. Food-derived natural antimicrobials offer advantages such as diminishing microbial loads and prolonging the shelf life of food products particularly prone to microbial spoilage. They not only enrich the foods with antioxidants but also help prevent microbial contamination, thereby prolonging their shelf life. Similarly, incorporating these natural antimicrobials into food packaging products extends the shelf life of meat products. Moreover, in agricultural practices, these natural antimicrobials act as eco-friendly pesticides, eliminating phytopathogenic microbes responsible for causing plant diseases. In medicine and pharmacology, they are being explored as potential therapeutic agents. This review article is based on current studies conducted in the last four years, evaluating the effectiveness of food-based natural antimicrobials in food, agriculture, medicine, and pharmacology.
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| 23. |
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| 24. |
- Sar, Taner, Postdoctor, 1989-, et al.
(författare)
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Repeated batch fermentation of immobilized E. coli expressing Vitreoscilla hemoglobin for long-term use
- 2017
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Ingår i: Bioengineered. - : Informa UK Limited. - 2165-5979 .- 2165-5987. ; 8:5, s. 651-660
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Tidskriftsartikel (refereegranskat)abstract
- This study describes an efficient and reusable process for ethanol production from medium containing whey powder, using alginate immobilized ethanologenic E. coli strains either expressing (TS3) or not expressing (FBR5) Vitreoscilla hemoglobin. Reuseabilities of the FBR5 and TS3 strains were investigated regarding their ethanol production capacities over the course of 15 successive 96-h batch fermentations. The ethanol production was fairly stable over the entire duration of the experiment, with strain TS3 maintaining a substantial advantage over strain FBR5. Storage of both strains in 2 different solutions for up to 60 d resulted in only a modest loss of ethanol production, with strain TS3 consistently outperforming strain FBR5 by a substantial amount. Strains stored for 15 or 30 d maintained their abilities to produce ethanol without dimunition over the course of 8 successive batch fermentations; again strain TS3 maintained a substantial advantage over strain FBR5 throughout the entire experiment. Thus, immobilization is a useful strategy to maintain the advantage in ethanol productivity afforded by expression of Vitreoscilla hemoglobin over long periods of time and large numbers of repeated batch fermentations, including, as in this case, using media with food processing wastes as the carbon source.
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| 25. |
- Unlu, Aise, et al.
(författare)
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Biofilm formation by Staphylococcus aureus strains and their control by selected phytochemicals
- 2018
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Ingår i: International Journal of Dairy Technology. - : Wiley. - 1364-727X .- 1471-0307. ; 71:3, s. 637-646
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Tidskriftsartikel (refereegranskat)abstract
- Biofilm formation by 30 Staphylococcus aureus dairy isolates and their control by phytochemicals were investigated. The majority of strains were biofilm positive by phenotypic analysis. The nuc and icaA genes were present in 30 and 27 strains, respectively. In addition, 13 strains were positive for all nuc, clfA, fnbA and icaA genes. The antimicrobial and antibiofilm activities of citral, cinnamaldehyde, eugenol, farnesol, limonene and terpinen-4-ol were also evaluated for seven strains. It was shown that the use of farnesol, cinnamaldehyde or terpinen-4-ol at a concentration of 2 mg/mL could be at least 80% effective on S. aureus strains and their biofilms.
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