| 1. |
- Remnestål, Julia, et al.
(författare)
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Association of CSF proteins with tau and amyloid beta levels in asymptomatic 70-year-olds
- 2021
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Ingår i: Alzheimer's Research & Therapy. - : BMC. - 1758-9193. ; 13:1
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Tidskriftsartikel (refereegranskat)abstract
- Background Increased knowledge of the evolution of molecular changes in neurodegenerative disorders such as Alzheimer's disease (AD) is important for the understanding of disease pathophysiology and also crucial to be able to identify and validate disease biomarkers. While several biological changes that occur early in the disease development have already been recognized, the need for further characterization of the pathophysiological mechanisms behind AD still remains. Methods In this study, we investigated cerebrospinal fluid (CSF) levels of 104 proteins in 307 asymptomatic 70-year-olds from the H70 Gothenburg Birth Cohort Studies using a multiplexed antibody- and bead-based technology. Results The protein levels were first correlated with the core AD CSF biomarker concentrations of total tau, phospho-tau and amyloid beta (A beta 42) in all individuals. Sixty-three proteins showed significant correlations to either total tau, phospho-tau or A beta 42. Thereafter, individuals were divided based on CSF A beta 42/A beta 40 ratio and Clinical Dementia Rating (CDR) score to determine if early changes in pathology and cognition had an effect on the correlations. We compared the associations of the analysed proteins with CSF markers between groups and found 33 proteins displaying significantly different associations for amyloid-positive individuals and amyloid-negative individuals, as defined by the CSF A beta 42/A beta 40 ratio. No differences in the associations could be seen for individuals divided by CDR score. Conclusions We identified a series of transmembrane proteins, proteins associated with or anchored to the plasma membrane, and proteins involved in or connected to synaptic vesicle transport to be associated with CSF biomarkers of amyloid and tau pathology in AD. Further studies are needed to explore these proteins' role in AD pathophysiology.
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| 2. |
- Remnestål, Julia, et al.
(författare)
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Association of CSF proteins with tau and amyloid β levels in asymptomatic 70-year-olds.
- 2021
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Ingår i: Alzheimer's research & therapy. - : Springer Science and Business Media LLC. - 1758-9193. ; 13:1
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Tidskriftsartikel (refereegranskat)abstract
- Increased knowledge of the evolution of molecular changes in neurodegenerative disorders such as Alzheimer's disease (AD) is important for the understanding of disease pathophysiology and also crucial to be able to identify and validate disease biomarkers. While several biological changes that occur early in the disease development have already been recognized, the need for further characterization of the pathophysiological mechanisms behind AD still remains.In this study, we investigated cerebrospinal fluid (CSF) levels of 104 proteins in 307 asymptomatic 70-year-olds from the H70 Gothenburg Birth Cohort Studies using a multiplexed antibody- and bead-based technology.The protein levels were first correlated with the core AD CSF biomarker concentrations of total tau, phospho-tau and amyloid beta (Aβ42) in all individuals. Sixty-three proteins showed significant correlations to either total tau, phospho-tau or Aβ42. Thereafter, individuals were divided based on CSF Aβ42/Aβ40 ratio and Clinical Dementia Rating (CDR) score to determine if early changes in pathology and cognition had an effect on the correlations. We compared the associations of the analysed proteins with CSF markers between groups and found 33 proteins displaying significantly different associations for amyloid-positive individuals and amyloid-negative individuals, as defined by the CSF Aβ42/Aβ40 ratio. No differences in the associations could be seen for individuals divided by CDR score.We identified a series of transmembrane proteins, proteins associated with or anchored to the plasma membrane, and proteins involved in or connected to synaptic vesicle transport to be associated with CSF biomarkers of amyloid and tau pathology in AD. Further studies are needed to explore these proteins' role in AD pathophysiology.
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| 3. |
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| 4. |
- Thul, Peter J., et al.
(författare)
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A subcellular map of the human proteome
- 2017
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Ingår i: Science. - : American Association for the Advancement of Science. - 0036-8075 .- 1095-9203. ; 356:6340
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Tidskriftsartikel (refereegranskat)abstract
- Resolving the spatial distribution of the human proteome at a subcellular level can greatly increase our understanding of human biology and disease. Here we present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localization of 12,003 human proteins at a single-cell level to 30 subcellular structures enabled the definition of the proteomes of 13 major organelles. Exploration of the proteomes revealed single-cell variations in abundance or spatial distribution and localization of about half of the proteins to multiple compartments. This subcellular map can be used to refine existing protein-protein interaction networks and provides an important resource to deconvolute the highly complex architecture of the human cell.
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| 5. |
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| 6. |
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Aktivism : En antologi om arbete för social och politisk förändring
- 2022
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Samlingsverk (redaktörskap) (refereegranskat)abstract
- Aktivism kan vara en livsstil, ett nödvändigt ont, en professionell praktik, en hoppfull intervention och en effekt av att bara befinna sig i världen. Men vad vill det säga att arbeta för social förändring i det senmoderna och (post) pandemiskasamhället? Vilka uttryck kan aktivism ta sig, vilka politiska utmaningar är mest akuta att adressera och hur ser relationer mellan akademiskt och aktivistiskt arbete ut? I denna antologi om aktivism och arbete för förändring i olika kontexter och på olika arenor presenterar vi nydanande forskning om aktivism och dess samhälleliga betydelser. Antologin innehåller såväl beskrivningar av nutida aktivistiska praktiker som reflektioner över vad aktivism kan innebära i termer av politisk och social förändring. Vår förhoppning är att bokens bidrag ska ge läsareunderlag för att se nya sammanhang mellan olika aktivistiska dagordningar och uttryck inom och utanför akademin och vidga förståelser av vad aktivism är och kan vara.
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| 7. |
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| 8. |
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| 9. |
- Ansari, Farhan, et al.
(författare)
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Hierarchical wood cellulose fiber/epoxy biocomposites : Materials design of fiber porosity and nanostructure
- 2015
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Ingår i: Composites. Part A, Applied science and manufacturing. - : Elsevier BV. - 1359-835X .- 1878-5840. ; 74, s. 60-68
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Tidskriftsartikel (refereegranskat)abstract
- Delignified chemical wood pulp fibers can be designed to have a controlled structure of cellulose fibril aggregates to serve as porous templates in biocomposites with unique properties. The potential of these fibers as reinforcement for an epoxy matrix (EP) was investigated in this work. Networks of porous wood fibers were impregnated with monomeric epoxy and cured. Microscopy images from ultramicrotomed cross sections and tensile fractured surfaces were used to study the distribution of matrix inside and around the fibers - at two different length scales. Mechanical characterization at different relative humidity showed much improved mechanical properties of biocomposites based on epoxy-impregnated fibers and they were rather insensitive to surrounding humidity. Furthermore, the mechanical properties of cellulose-fiber biocomposites were compared with those of cellulose-nanofibril (CNF) composites; strong similarities were found between the two materials. The reasons for this, some limitations and the role of specific surface area of the fiber are discussed.
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| 10. |
- Borgström, Anna, et al.
(författare)
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Wetlands as a Local Scale Management Tool to Reduce Algal Growth Potential
- 2022
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Ingår i: Wetlands. - : Springer Science and Business Media LLC. - 0277-5212 .- 1943-6246. ; 42
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Tidskriftsartikel (refereegranskat)abstract
- Recent land-use changes have led to a significant loss of natural wetlands worldwide resulting in increased amounts of organic and inorganic compounds reaching lakes and coastal areas. In turn, this has led to an increased algal growth, and subsequently the risk of algal blooms and deteriorated water quality. The capacity of wetlands to retain nutrients is well-known, suggesting that constructed wetlands may be a potential management strategy to mitigate algal blooms in downstream waters, although little is known about seasonal variation in reduction of algal growth potential. Therefore, in a long-term study, we experimentally evaluated the efficiency of seven wetlands to reduce the algal growth potential by comparing the growth in cultures containing 50:50 wetland water from the inlet or outlet and water from a eutrophic lake as a standard inoculum. We show that the majority of the wetlands have a considerable potential to reduce algal growth potential, with up to 89% for cyanobacteria and 73% for green algae. However, there were strong temporal variations in efficiency within, as well as between wetlands. Specifically, we show that the potential to reduce algal growth (standardized conditions) was generally higher in winter than in summer. In addition, the wetlands showed different efficiency in reducing the growth potential of cyanobacteria and green algae. Taken together, our results show that wetlands have a considerable potential to reduce algal growth potential, suggesting that they are an efficient local-scale tool in reducing the risk of algal blooms especially from a future climate change perspective.
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| 11. |
- Borgström, Anna, et al.
(författare)
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Wetlands as a potential multifunctioning tool to mitigate eutrophication and brownification
- 2024
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Ingår i: Ecological Applications. - 1051-0761 .- 1939-5582.
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Tidskriftsartikel (refereegranskat)abstract
- Eutrophication and brownification are ongoing environmental problems affecting aquatic ecosystems. Due to anthropogenic changes, increasing amounts of organic and inorganic compounds are entering aquatic systems from surrounding catchment areas, increasing both nutrients, total organic carbon (TOC), and water color with societal, as well as ecological consequences. Several studies have focused on the ability of wetlands to reduce nutrients, whereas data on their potential to reduce TOC and water color are scarce. Here we evaluate wetlands as a potential multifunctional tool for mitigating both eutrophication and brownification. Therefore, we performed a study for 18 months in nine wetlands allowing us to estimate the reduction in concentrations of total nitrogen (TN), total phosphorus (TP), TOC and water color. We show that wetland reduction efficiency with respect to these variables was generally higher during summer, but many of the wetlands were also efficient during winter. We also show that some, but not all, wetlands have the potential to reduce TOC, water color and nutrients simultaneously. However, the generalist wetlands that reduced all four parameters were less efficient in reducing each of them than the specialist wetlands that only reduced one or two parameters. In a broader context, generalist wetlands have the potential to function as multifunctional tools to mitigate both eutrophication and brownification of aquatic systems. However, further research is needed to assess the design of the generalist wetlands and to investigate the potential of using several specialist wetlands in the same catchment.
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| 12. |
- Chin, Chui-Yoke, et al.
(författare)
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Francisella FlmX broadly affects lipopolysaccharide modification and virulence
- 2021
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Ingår i: Cell Reports. - : Elsevier. - 2211-1247. ; 35:11
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Tidskriftsartikel (refereegranskat)abstract
- The outer membrane protects Gram-negative bacteria from the host environment. Lipopolysaccharide (LPS), a major outer membrane constituent, has distinct components (lipid A, core, O-antigen) generated by specialized pathways. In this study, we describe the surprising convergence of these pathways through FlmX, an uncharacterized protein in the intracellular pathogen Francisella. FlmX is in the flippase family, which includes proteins that traffic lipid-linked envelope components across membranes. flmX deficiency causes defects in lipid A modification, core remodeling, and O-antigen addition. We find that an F. tularensis mutant lacking flmX is >1,000,000-fold attenuated. Furthermore, FlmX is required to resist the innate antimicrobial LL-37 and the antibiotic polymyxin. Given FlmX's central role in LPS modification and its conservation in intracellular pathogens Brucella, Coxiella, and Legionella, FlmX may represent a novel drug target whose inhibition could cripple bacterial virulence and sensitize bacteria to innate antimicrobials and antibiotics.
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| 13. |
- Drotz, Mattias, et al.
(författare)
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A laboratory investigation of Superheated Steam Dried pulps
- 2022
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Ingår i: TAPPICon Conference 2022. - : TAPPI Press.
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Konferensbidrag (refereegranskat)abstract
- Superheated steam drying is used commercially for lumber, coal, peat, sludges, but has limited installations towards market pulp applications. The technology has the potential for higher drying rates, lower energy consumption, better product quality, and safe operation. In this investigation, a superheated steam dryer (EXERGYPSSD®) was used for evaluation of wet pulp samples collected from pulp mills for comparison with conventional drying techniques. The evaluation of the superheated steam dried pulps indicated that there are potential quality benefits with the technology. Improvements of bulk and absorption capacity was achieved, but more laboratory, and pilot tests are needed to optimize the technology even more regarding pressure, temperature, and residence time in the dryer.
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| 14. |
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| 15. |
- Fagerberg, Linn, et al.
(författare)
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Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics
- 2014
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Ingår i: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 13:2, s. 397-406
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Tidskriftsartikel (refereegranskat)abstract
- Global classification of the human proteins with regards to spatial expression patterns across organs and tissues is important for studies of human biology and disease. Here, we used a quantitative transcriptomics analysis (RNA-Seq) to classify the tissue-specific expression of genes across a representative set of all major human organs and tissues and combined this analysis with antibody- based profiling of the same tissues. To present the data, we launch a new version of the Human Protein Atlas that integrates RNA and protein expression data corresponding to 80% of the human protein-coding genes with access to the primary data for both the RNA and the protein analysis on an individual gene level. We present a classification of all human protein-coding genes with regards to tissue-specificity and spatial expression pattern. The integrative human expression map can be used as a starting point to explore the molecular constituents of the human body.
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| 16. |
- Fagerberg, Linn, et al.
(författare)
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Contribution of antibody-based protein profiling to the human chromosome-centric proteome project (C-HPP)
- 2013
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Ingår i: Journal of Proteome Research. - : American Chemical Society (ACS). - 1535-3893 .- 1535-3907. ; 12:6, s. 2439-2448
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Tidskriftsartikel (refereegranskat)abstract
- A gene-centric Human Proteome Project has been proposed to characterize the human protein-coding genes in a chromosome-centered manner to understand human biology and disease. Here, we report on the protein evidence for all genes predicted from the genome sequence based on manual annotation from literature (UniProt), antibody-based profiling in cells, tissues and organs and analysis of the transcript profiles using next generation sequencing in human cell lines of different origins. We estimate that there is good evidence for protein existence for 69% (n = 13985) of the human protein-coding genes, while 23% have only evidence on the RNA level and 7% still lack experimental evidence. Analysis of the expression patterns shows few tissue-specific proteins and approximately half of the genes expressed in all the analyzed cells. The status for each gene with regards to protein evidence is visualized in a chromosome-centric manner as part of a new version of the Human Protein Atlas (www.proteinatlas.org).
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| 17. |
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| 18. |
- Forslund, Anna-Lena, 1964-, et al.
(författare)
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The type IV pilin, PilA, is required for full virulence of Francisella tularensis subspecies tularensis
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Annan publikation (populärvet., debatt m.m.)abstract
- Background: All four Francisella tularensis subspecies possess gene clusters with potential to express type IV pili (Tfp). These clusters include putative pilin genes, as well as pilB, pilC and pilQ, required for secretion and assembly of Tfp. A hallmark of Tfp is the ability to retract the pilus upon surface contact, a property mediated by the ATPase PilT. Interestingly, out of the two major human pathogenic subspecies only the highly virulent type A strains have a functional pilT gene.Results: In a previous study, we were able to show that one pilin gene, pilA, was essential for virulence of a type B strain in a mouse infection model. In this work we have examined the role of several pilin genes in the virulence of the pathogenic type A strain SCHU S4. pilA, pilC, pilQ, and pilT were mutated by in-frame deletion mutagenesis. Interestingly, when mice were infected with a mixture of each mutant strain and the wild-type strain, the pilA, pilC and pilQ mutants were out-competed, while the pilT mutant was equally competitive as the wild-type.Conclusions: This suggests that expression and surface localisation of PilA contribute to virulence in the highly virulent type A strain, while PilT was dispensable for virulence in the mouse infection model.
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| 19. |
- Hemdan, Tammer, et al.
(författare)
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Stathmin-1 is a promising prognostic factor and potential therapeutic target in urinary bladder cancer
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Aim: The oncoprotein 18/stathmin 1 (STMN1), involved in cell cycle progression and cell migration, has been reported to be expressed in several types of cancer, and is associated with clinical outcome in e.g. breast and liver cancer. The aims in this study were to investigate the clinical significance of STMN1 and to examine if STMN1 might be a possible therapeutic target in urinary bladder cancer.Experimental design: Immunohistochemical analyses of STMN1 protein expression were performed in a wide-range tissue microarray (115 Ta-, 115 T1-, 112 T2-4-tumors) and in a metastatic primary tumor/matched metastasis-material (90 patients). In the T24 cell line, the effect of STMN1 on cell proliferation was evaluated by inhibiting the cellular expression of STMN using STMN1-siRNA.Results: Patients with T1- or muscle-invasive disease exhibiting high expression of the STMN1 protein had a poorer overall survival (OS) and disease specific survival (DSS). In a multivariate analysis adjusting for stage, age and gender the results were for T2-T4 patients: OS (HR=1.77 95% CI 1.02-3.07; p=0.04) and DSS (HR=2.04 95% CI 1.13-3.68; p=0.02); for T1-4 patients: DSS (HR=1.83 95% CI 1.09-3.08; p=0.02). In the metastatic bladder cancer material, the majority of the patients with one metastasis (69%) and with several matched metastases (70%) were STMN1-positive in both the primary tumor and the matched metastases. Moreover, the ability of the urinary bladder cancer cell line to grow was significantly reduced after 72 hours (p<0.0001) when transfecting the cells with a siRNA targeting STMN1.Conclusion: Our results suggest that STMN1 protein-expression has a potential both as a prognostic marker and a novel treatment target in urinary bladder cancer.
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| 20. |
- Hemdan, Tammer, et al.
(författare)
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The prognostic value and therapeutic target role of stathmin-1 in urinary bladder cancer
- 2014
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Ingår i: British Journal of Cancer. - : Springer Science and Business Media LLC. - 0007-0920 .- 1532-1827. ; 111:6, s. 1180-1187
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Tidskriftsartikel (refereegranskat)abstract
- Background:The oncoprotein-18/stathmin 1 (STMN1), involved in cell progression and migration, is associated with clinical outcome in breast cancer. Here we aim to investigate its clinical significance in urinary bladder cancer and its possibilities as a therapeutic target.Methods:Immunohistochemical analyses of STMN1 protein expression were performed in three patient cohorts: cohort I (n=115 Ta, n=115 T1, n=112 T2-4 stages), cohort II, based on randomised controlled trials (n=239 T1-T4), and cohort III of primary tumour/matched metastasis (n=90 T1-T4). The effects of STMN1 on cell proliferation and migration were evaluated in the urinary bladder cancer cell line, T24, by inhibiting STMN1-cellular expression using siRNA.Results:In cohort I, high STMN1 expression correlated to shorter disease-specific survival hazard ratio (HR)=2.04 (95% confidence interval (CI) 1.13-3.68; P=0.02), elevated p53- (P<0.001) and Ki67-protein levels (P<0.001). The survival result was validated in cohort II: HR=1.76 (95% CI 1.04-2.99; P=0.03). In the metastatic bladder cancer material, 70% of the patients were STMN1-positive in both the primary tumour and matched metastases. In vitro, the growth and migration of the T24 cells were significantly reduced (P<0.01, P<0.0001, respectively), when transfecting the cells with STMN1-siRNA.Conclusions:STMN1 protein expression has prognostic significance but is primarily a potential treatment target in urinary bladder cancer.
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| 21. |
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| 22. |
- Kauppi, Anna M., et al.
(författare)
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Metabolites in Blood for Prediction of Bacteremic Sepsis in the Emergency Room
- 2016
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Ingår i: PLOS ONE. - : Public Library Science. - 1932-6203. ; 11:1
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Tidskriftsartikel (refereegranskat)abstract
- A metabolomics approach for prediction of bacteremic sepsis in patients in the emergency room (ER) was investigated. In a prospective study, whole blood samples from 65 patients with bacteremic sepsis and 49 ER controls were compared. The blood samples were analyzed using gas chromatography coupled to time-of-flight mass spectrometry. Multivariate and logistic regression modeling using metabolites identified by chromatography or using conventional laboratory parameters and clinical scores of infection were employed. A predictive model of bacteremic sepsis with 107 metabolites was developed and validated. The number of metabolites was reduced stepwise until identifying a set of 6 predictive metabolites. A 6-metabolite predictive logistic regression model showed a sensitivity of 0.91(95% CI 0.69-0.99) and a specificity 0.84 (95% CI 0.58-0.94) with an AUC of 0.93 (95% CI 0.89-1.01). Myristic acid was the single most predictive metabolite, with a sensitivity of 1.00 (95% CI 0.85-1.00) and specificity of 0.95 (95% CI 0.74-0.99), and performed better than various combinations of conventional laboratory and clinical parameters. We found that a metabolomics approach for analysis of acute blood samples was useful for identification of patients with bacteremic sepsis. Metabolomics should be further evaluated as a new tool for infection diagnostics.
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| 23. |
- Kylin, Maria, et al.
(författare)
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Inclusive Residential Areas - a report on a fieldtrip to the Netherlands
- 2022
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Rapport (populärvet., debatt m.m.)abstract
- This report discusses seven residential areas in the Netherlands from the perspective of social inclusiveness – EVA Lanxmeer, Park Rosendaal, Bijlmermeer, Diagoon Housing, Spangen, Ypenburg and Java Island. The material was collected during a field trip to the Netherlands in spring 2020, as part of a Call for Ideas initiative from SLU Landscape. Jointly, we have compiled our experiences from the field trip in this publication, for inspirational use within educational settings.
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| 24. |
- Källblad, Anna, et al.
(författare)
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Hoppsa Universum – An interactive dance installation for children
- 2008
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Ingår i: Proceedings of New Interfaces for Musical Expression (NIME), Genova, 2008. ; , s. 128-133
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Konferensbidrag (refereegranskat)abstract
- It started with an idea to create an empty space in which you activated music and light as you moved around. In responding to the music and lighting you would activate more or different sounds and thereby communicate with the space through your body. This led to an artistic research project in which children’s spontaneous movement was observed, a choreography made based on the children’s movements and music written and recorded for the choreography. This music was then decomposed and choreographed into an empty space at Botkyrka konsthall creating an interactive dance installation. It was realized using an interactive sound and light system in which 5 video cameras were detecting the motion in the room connected to a 4-channel sound system and a set of 14 light modules. During five weeks people of all ages came to dance and move around in the installation. The installation attracted a wide range of people of all ages and the tentative evaluation indicates that it was very positively received and that it encouraged free movement in the intended way. Besides observing the activity in the installation interviews were made with schoolchildren age 7 who had participated in the installation.
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| 25. |
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