| 1. |
- Gomis-Fons, Joaquín, et al.
(author)
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Model-based design and control of a small-scale integrated continuous end-to-end mAb platform
- 2020
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In: Biotechnology progress (Print). - : John Wiley and Sons Inc.. - 8756-7938 .- 1520-6033.
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Journal article (peer-reviewed)abstract
- A continuous integrated bioprocess available from the earliest stages of process development allows for an easier, more efficient and faster development and characterization of an integrated process as well as production of small-scale drug candidates. The process presented in this article is a proof-of-concept of a continuous end-to-end monoclonal antibody production platform at a very small scale based on a 200 ml alternating tangential flow filtration perfusion bioreactor, integrated with the purification process with a model-based design and control. The downstream process, consisting of a periodic twin-column protein A capture, a virus inactivation, a CEX column and an AEX column, was compactly implemented in a single chromatography system, with a purification time of less than 4 hr. Monoclonal antibodies were produced for 17 days in a high cell density perfusion culture of CHO cells with titers up to 1.0 mg/ml. A digital twin of the downstream process was created by modelling all the chromatography steps. These models were used for real-time decision making by the implementation of control strategies to automatize and optimize the operation of the process. A consistent glycosylation pattern of the purified product was ensured by the steady state operation of the process. Regarding the removal of impurities, at least a 4-log reduction in the HCP levels was achieved. The recovery yield was up to 60%, and a maximum productivity of 0.8 mg/ml/day of purified product was obtained.
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| 4. |
- Lindström, H, et al.
(author)
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Electron transport properties in dye-sensitized nanoporous-nanocrystalline TiO2 films
- 1996
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In: JOURNAL OF PHYSICAL CHEMISTRY. - : AMER CHEMICAL SOC. ; 100:8, s. 3084-3088
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Journal article (other academic/artistic)abstract
- Spectral response measurements have been performed on dye-sensitized nanoporous-nanocrystalline TiO2 photoelectrodes. The effects of film thickness, electron scavengers in the electrolyte, and surface treatment of the nanocrystalline film were studied by
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| 5. |
- Lindström, H, et al.
(author)
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Li+ ion insertion in TiO2 (anatase) .1. Chronoamperometry on CVD films and nanoporous films
- 1997
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In: JOURNAL OF PHYSICAL CHEMISTRY B. - : AMER CHEMICAL SOC. - 1089-5647. ; 101:39, s. 7710-7716
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Journal article (other academic/artistic)abstract
- Electrochemical insertion of lithium in nanoporous and CVD samples of TiO2 (anatase) was studied by chronoamperometry. The currents following cathodic and anodic potential steps were monitored as a function of film thickness, temperature, and electrolyte
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| 6. |
- Lindström, H, et al.
(author)
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Li+ ion insertion in TiO2 (anatase) .2. Voltammetry on nanoporous films
- 1997
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In: JOURNAL OF PHYSICAL CHEMISTRY B. - : AMER CHEMICAL SOC. - 1089-5647. ; 101:39, s. 7717-7722
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Journal article (other academic/artistic)abstract
- Electrochemical properties of Li+ ion insertion in nanoporous TiO2 (anatase) electrodes were studied by voltammetry. Linear and cyclic potential scans were recorded as a function of electrolyte concentration, film thickness, and temperature. The currents
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