| 1. |
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| 2. |
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| 3. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 4. |
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| 5. |
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| 6. |
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| 7. |
- Ablikim, M., et al.
(författare)
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Amplitude analysis of the KSKS system produced in radiative J /psi decays
- 2018
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Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 98:7
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Tidskriftsartikel (refereegranskat)abstract
- An amplitude analysis of the KSKS system produced in radiative J/psi decays is performed using the (1310.6 +/- 7.0) x 10(6) nip decays collected by the BESIII detector. Two approaches are presented. A mass-dependent analysis is performed by parametrizing the KSKS invariant mass spectrum as a sum of Breit-aligner line shapes. Additionally, a mass-independent analysis is performed to extract a piecewise function that describes the dynamics of the KSKS system while making minimal assumptions about the properties and number of poles in the amplitude. The dominant amplitudes in the mass-dependent analysis include the f(0)(1710), f(0)(2200), and f(2)'(1525). The mass-independent results, which are made available as input for further studies, are consistent with those of the mass-dependent analysis and are useful for a systematic study of hadronic interactions. The branching fraction of radiative J/psi decays to KSKS is measured to be (8.1 +/- 0.4) x 10(-4), where the uncertainty is systematic and the statistical uncertainty is negligible.
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| 8. |
- Ablikim, M., et al.
(författare)
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Measurement of e(+)e(-) -> K(K)over-barJ/psi cross sections at center-of-mass energies from 4.189 to 4.600 GeV
- 2018
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Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 97:7
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Tidskriftsartikel (refereegranskat)abstract
- We investigate the process e(+)e(-) -> K (K) over barJ/psi at center-of-mass energies from 4.189 to 4.600 GeV using 4.7 fb(-1) of data collected by the BESIII detector at the BEPCII collider. The Born cross sections for the reactions e(+)e(-) -> K(+)K(-)J/psi and K(S)(0)K(S)(0)J/psi are measured as a function of center-of-mass energy. The energy dependence of the cross section for e(+)e(-) -> K(+)K(-)J/psi is shown to differ from that for pi(+)pi(-)J/psi in the region around the Y(4260). In addition, there is evidence for a structure around 4.5 GeV in the e(+)e(-) -> K(+)K(-)J/psi cross section that is not present in pi(+)pi(-)J/psi.
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| 9. |
- Ablikim, M., et al.
(författare)
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Measurement of the absolute branching fraction of D*(s0) (2317)(+/-) -> pi D-0(s)+/-
- 2018
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Ingår i: Physical Review D. - : American Physical Society. - 2470-0010 .- 2470-0029. ; 97:5
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Tidskriftsartikel (refereegranskat)abstract
- The process e(+)e(-) -> D*D-+(s)*(s0) (2317)(-) + c.c. is observed for the first time with the data sample of 567 pb(-1) collected with the BESIII detector operating at the BEPCII collider at a center-of-mass energy root s = 4.6 GeV. The statistical significance of the D*(s0) (2317)(+/-) signal is 5.8 sigma and the mass is measured to be (2318.3 +/- 1.2 +/- 1.2) MeV/c(2). The absolute branching fraction B(D*(s0) (2317)(+/-) -> pi D-0(s)+/-) is measured as 1.00(-0.14)(+0.00) (stat)(-0.14)(+0.00) (syst) for the first time. The uncertainties are statistical and systematic, respectively.
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| 10. |
- Ablikim, M., et al.
(författare)
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Measurement of the Absolute Branching Fraction of the Inclusive Decay Lambda(+)(c) -> Lambda plus X
- 2018
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Ingår i: Physical Review Letters. - : AMER PHYSICAL SOC. - 0031-9007 .- 1079-7114. ; 121:6
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Tidskriftsartikel (refereegranskat)abstract
- Based on an e(+)e(-) collision data sample corresponding to an integrated luminosity of 567 pb(-1) taken at the center-of-mass energy of root s = 4.6 GeV with the BESIII detector, we measure the absolute branching fraction of the inclusive decay Lambda(+)(c) -> Lambda + X to be B(Lambda(+)(c) -> Lambda + X) = (38.2(-2.2)(+2.8) +/- 0.9)% using the double-tag method, where X refers to any possible final state particles. In addition, we search for direct CP violation in the charge asymmetry of this inclusive decay for the first time, and obtain A(CP) [B(Lambda(+)(c) -> Lambda + X) - B((Lambda) over bar (-)(c) -> (Lambda) over bar + X)]/[B(Lambda(+)(c) -> Lambda + X) + B((Lambda) over bar (-)(c) -> (Lambda) over bar + X)] = (2.1(-6.6)(+7.0) +/- 1.6)%, a statistically limited result with no evidence of CP violation.
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| 11. |
- Ablikim, M., et al.
(författare)
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Measurements of the branching fractions of the singly Cabibbo-suppressed decays D0→ωη, η(')π0 and η(')η
- 2018
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Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 97:5
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Tidskriftsartikel (refereegranskat)abstract
- By analyzing a data sample of 2.93 fb(-1) collected at root s = 3.773 GeV with the BESIII detector operated at the BEPCII storage rings, we measure the branching fractions B(D-0 -> omega eta) = (2.15 +/- 0.17(stat) +/- 0.15(sys)) x 10(-3), B(D-0 ->eta pi(0)) = (0.58 +/- 0.05(stat) +/- 0.05(sys)) x 10(-3), B(D-0 ->eta'pi(0)) = (0.93 +/- 0.11(stat) +/- 0.09(sys)) x 10(-3), B(D-0 -> eta eta) = (2.20 +/- 0.07(stat) +/- 0.06(sys)) x 10(-3) and B(D-0 -> eta'eta) = (0.94 degrees +/- 0.25(stat) +/- 0.11(sys)) x 10(-3). We note that B(D-0 -> omega eta) is measured for the first time and that B(D-0 -> eta eta) is measured with much improved precision.
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| 12. |
- Ablikim, M., et al.
(författare)
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Observation of h(1)(1380) in the J/psi -> eta ' K(K)over-bar pi decay
- 2018
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Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 98:7
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Tidskriftsartikel (refereegranskat)abstract
- Using 1.31 x 10(9) J/psi events collected by the BESIII detector at the BEPCII e(+)e(-) collider, we report the first observation of the h(1)(1380) in J/psi -> eta'h(1)(1380) with a significance of more than ten standard deviations. The mass and width of the possible axial-vector strangeonium candidate h(1)(1380) are measured to be M = (1423.2 +/- 2.1 +/- 7.3) MeV/c(2) and Gamma = (90.3 +/- 9.8 +/- 17.5) MeV. The product branching fractions, assuming no interference, are determined to be B(J/psi -> eta'h(1)(1380)) x B(h(1)(1380) -> K*(892)K-+(-)+c.c.) = (1.51 +/- 0.09 +/- 0.21) x 10(-4) in eta'K+K-pi(0) mode and B(J/psi -> eta'h(1)(1380)) x B(h(1)(1380) -> K*(892)(K) over bar +c.c.) =(2.16 +/- 0.12 +/- 0.29) x 10(-4) in eta'(KSK +/-)-K-0 pi(-/+) mode. The first uncertainties are statistical and the second are systematic. Isospin symmetry violation is observed in the decays h(1)(1380) K*(892)K-+(-) + c.c. and h(1)(1380) -> K*(892)(0)(K) over bar (0) + c.c.. Based on the measured h(1)(1380) mass, the mixing angle between the states h(1)(1170) and h(1)(1380) is determined to be (35.9 +/- 2.6)degrees, consistent with theoretical expectations.
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| 13. |
- Ablikim, M., et al.
(författare)
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Study of the decays D+-> eta(('))e(+)nu(e)
- 2018
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Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 97:9
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Tidskriftsartikel (refereegranskat)abstract
- The charm semileptonic decays D+ -> eta e(+)nu(e) and D+ -> eta'e(+)nu(e) are studied with a sample of e(+)e(-) collision data corresponding to an integrated luminosity of 2.93 fb(-1) collected at root s = 3.773 GeV with the BESIII detector. We measure the branching fractions for D+ -> eta e(+)upsilon(e) to be (10.74 +/- 0.81 +/- 0.51)x10(-4), and for D+ -> eta'e(+)nu(e) to be (1.91 +/- 0.51 +/- 0.13) x 10(-4), where the uncertainties are statistical and systematic, respectively. In addition, we perform a measurement of the form factor in the decay D+ -> eta e(+)nu(e) . All the results are consistent with those obtained by the CLEO-c experiment.
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| 14. |
- Ablikim, M., et al.
(författare)
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Measurement of e(+)e(-) -> D(D)over-bar cross sections at the psi(3770) resonance
- 2018
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Ingår i: Chinese Physics C. - : IOP PUBLISHING LTD. - 1674-1137 .- 2058-6132. ; 42:8
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Tidskriftsartikel (refereegranskat)abstract
- We report new measurements of the cross sections for the production of D (D) over bar final states at the psi(3770) resonance. Our data sample consists of an integrated luminosity of 2.93 fb(-1) of e(+)e(-) annihilation data produced by the BEPCII collider and collected and analyzed with the BESIII detector. We exclusively reconstruct three D-0 and six D+ hadronic decay modes and use the ratio of the yield of fully reconstructed D (D) over bar events ("double tags") to the yield of all reconstructed D or (D) over bar mesons ("single tags") to determine the number of D-0(D) over bar (0) and D+D- events, benefiting from the cancellation of many systematic uncertainties. Combining these yields with an independent determination of the integrated luminosity of the data sample, we find the cross sections to be sigma(e(+)e(-) -> D-0(D) over bar (0)(-) )=(3.615 +/- 0.010 +/- 0.038) nb and sigma(e(+)e(-) -> D+D-)=(2.830 +/- 0.011 +/- 0.026) nb, where the uncertainties are statistical and systematic, respectively.
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| 15. |
- Ablikim, M., et al.
(författare)
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Measurement of singly Cabibbo-suppressed decays D-0 → π0π0π0, π0π0η, π0ηη and ηηη
- 2018
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Ingår i: Physics Letters B. - : ELSEVIER SCIENCE BV. - 0370-2693 .- 1873-2445. ; 781, s. 368-375
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Tidskriftsartikel (refereegranskat)abstract
- Using a data sample of e(+)e(-) collision data corresponding to an integrated luminosity of 2.93 fb(-1) collected with the BESIII detector at a center-of-mass energy of root s = 3.773 GeV, we search for the singly Cabibbo-suppressed decays D-0 -> pi(0)pi(0)pi(0), pi(0)pi(0)eta, pi(0)eta eta and eta eta eta using the double tag method. The absolute branching fractions are measured to be B(D-0 -> pi(0)pi(0)pi(0)) = (2.0 +/- 0.4 +/- 0.3) x 10(-4), B(D-0 -> pi(0)pi(0)eta) = (3.8 +/- 1.1 +/- 0.7) x 10(-4) and B(D-0 -> pi(0)eta eta) = (7.3 +/- 1.6 +/- 1.5) x 10(-4) with the statistical significances of 4.8 sigma, 3.8 sigma and 5.5 sigma, respectively, where the first uncertainties are statistical and the second ones systematic. No significant signal of D-0 -> eta eta eta is found, and the upper limit on its decay branching fraction is set to be B(D-0 -> eta eta eta) < 1.3 x 10(-4) at the 90% confidence level.
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| 16. |
- Ablikim, M., et al.
(författare)
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Measurement of the integrated Luminosities of cross-section scan data samples around the psi(3770) mass region
- 2018
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Ingår i: Chinese Physics C. - : SCIENCE PRESS. - 1674-1137 .- 2058-6132. ; 42:6
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Tidskriftsartikel (refereegranskat)abstract
- To investigate the nature of the psi(3770) resonance and to measure the cross section for e(+)e(-) -> D (D) over bar, a cross-section scan data sample, distributed among 41 center-of-mass energy points from 3.73 to 3.89 GeV, was taken with the BESIII detector operated at the BEPCII collider in the year 2010. By analyzing the large angle Bhabha scattering events, we measure the integrated luminosity of the data sample at each center-of-mass energy point. The total integrated luminosity of the data sample is 76.16 +/- 0.04 +/- 0.61 pb(-1), where the first uncertainty is statistical and the second systematic.
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| 17. |
- Ablikim, M., et al.
(författare)
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Measurements of absolute branching fractions for D mesons decays into two pseudoscalar mesons
- 2018
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Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 97:7
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Tidskriftsartikel (refereegranskat)abstract
- Using a data sample of e(+)e(-) collision data with an integrated luminosity of 2.93 fb(-1) taken at the center-of-mass energy root s = 3.773 GeV with the BESIII detector operating at the BEPCII storage rings, we measure the absolute branching fractions of the two-body hadronic decays D+ -> pi(+)pi(0), K+pi(0), pi(+)eta., K+eta., pi(+)eta', K+eta', K-S(0)pi(+), (KSK+)-K-0, and D-0 -> pi(+)pi(-), K+ K-, K--/+pi(+/-), K-S(0)pi(0), K-S(0)eta, K-S(0)eta' Our results are consistent with previous measurements within uncertainties. Among them, the branching fractions for D+-> pi(+)pi(0), K+pi(0), pi(+)eta, pi(+)eta', (KSK+)-K-0, (KSK+)-K-0 and D-0 -> K-S(0)pi(0), K-S(0)eta, K-S(0)eta' are determined with improved precision compared to the world average values.
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| 18. |
- Ablikim, M., et al.
(författare)
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Observation of e(+)e(-) -> phi chi(c1) and phi chi(c2) at root s=4.600 GeV
- 2018
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Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 97:3
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Tidskriftsartikel (refereegranskat)abstract
- Using a data sample collected with the BESIII detector operating at the BEPCII storage ring at a center-of-mass energy of root s = 4.600 GeV, we search for the production of e(+)e(-) -> phi chi(c0,1,2). A search is also performed for the charmonium-like state X(4140) in the radiative transition e(+)e(-) -> gamma X(4140) with X(4140) subsequently decaying into phi J/psi The processes e(+)e(-) -> phi chi(c1) and phi chi(c2) are observed for the first time, each with a statistical significance of more than 10 sigma, and the Born cross sections are measured to be (4.2(-1.0)(+1.7) +/- 0.3) and (6.7(-1.7)(+3.4) +/- 0.5) pb, respectively, where the first uncertainties are statistical and the second systematic. No significant signals are observed for e(+)e(-) -> phi chi(c0) and e(+)e(-) -> gamma X(4140) and upper limits on the Born cross sections at 90% C. L. are provided at root s = 4.600 GeV.
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| 19. |
- Ablikim, M., et al.
(författare)
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Observation of χc2→η′η′ and χc0,2→ηη′
- 2017
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Ingår i: Physical Review D. - 2470-0010 .- 2470-0029. ; 96:11
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Tidskriftsartikel (refereegranskat)abstract
- Using a sample of 448.1×106 ψ(3686) events collected with the BESIII detector in 2009 and 2012, we study the decays χc0,2→η′η′ and ηη′. The decays χc2→η′η′, χc0→ηη′ and χc2→ηη′ are observed for the first time with statistical significances of 9.6σ, 13.4σ and 7.5σ, respectively. The branching fractions are determined to be B(χc0→η′η′)=(2.19±0.03±0.14)×10−3, B(χc2→η′η′)=(4.76±0.56±0.38)×10−5, B(χc0→ηη′)=(8.92±0.84±0.65)×10−5 and B(χc2→ηη′)=(2.27±0.43±0.25)×10−5, where the first uncertainties are statistical and the second are systematic. The precision for the measurement of B(χc0→η′η′) is significantly improved compared to previous measurements. Based on the measured branching fractions, the role played by the doubly and singly Okubo-Zweig-Iizuka disconnected transition amplitudes for χc0,2 decays into pseudoscalar meson pairs can be clarified.
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| 20. |
- Ablikim, M., et al.
(författare)
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Observation of ψ(3686)→n¯n and improved measurement of ψ(3686)→p¯p
- 2018
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Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 98:3
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Tidskriftsartikel (refereegranskat)abstract
- We observe the decay psi (3686) -> n (n) over bar for the first time and measure psi (3686) -> p (p) over bar with improved accuracy by using 1.07 x 10(8) psi (3686) events collected with the BESIII detector. The measured branching fractions are B(psi(3686) -> n (n) over bar) = (3.06 +/- 0.06 +/- 0.14) x 10(-4) and B(psi(3686) -> p (p) over bar) = (3.05 +/- 0.02 +/- 0.12) x 10(-4). Here, the first uncertainties are statistical, and the second ones are systematic. With the hypothesis that the polar angular distributions of the neutron and proton in the center-of-mass system obey 1 + alpha cos(2) theta, we determine the a parameters to be alpha(n (n) over bar) = 0.68 +/- 0.12 +/- 0.11 and alpha(p (p) over bar) = 1.03 +/- 0.06 +/- 0.03 for psi(3686) -> n (n) over bar and psi(3686) -> p (p) over bar, respectively.
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| 21. |
- Ablikim, M., et al.
(författare)
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Precision Measurement of the e(+)e(-) -> Lambda(+)(c)(Lambda)over-bar(c)(-) Cross Section Near Threshold
- 2018
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Ingår i: Physical Review Letters. - : American Physical Society. - 0031-9007 .- 1079-7114. ; 120:13
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Tidskriftsartikel (refereegranskat)abstract
- The cross section of the e(+)e(-) -> Lambda(+)(c)(Lambda) over bar (-)(c) process is measured with unprecedented precision using data collected with the BESIII detector at root s = 4574.5, 4580.0, 4590.0 and 4599.5 MeV. The nonzero cross section near the Lambda(+)(c)(Lambda) over bar (-)(c) production threshold is cleared. At center-of-mass energies root s = 4574.5 and 4599.5 MeV, the higher statistics data enable us to measure the Lambda(c) polar angle distributions. From these, the.c electric over magnetic form-factor ratios (vertical bar G(E)/G(M)vertical bar) are measured for the first time. They are found to be 1.14 +/- 0.14 +/- 0.07 and 1.23 +/- 0.05 +/- 0.03, respectively, where the first uncertainties are statistical and the second are systematic.
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| 22. |
- Ablikim, M., et al.
(författare)
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Search for the rare decays J/ψ→D0e+e−+c.c. and ψ(3686)→D0e+e−+c.c.
- 2017
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Ingår i: Physical Review D. - 2470-0010 .- 2470-0029. ; 96:11
-
Tidskriftsartikel (refereegranskat)abstract
- Using the data samples of (1310.6 +/- 7.2) x 10(6) J/psi events and (448.1 +/- 2.9) x 10(6) psi(3686) events collected with the BESIII detector, we search for the rare decays J/psi -> D(0)e(+) e(-) + c.c. and psi(3686) -> D(0)e(+) e(-) + c.c. No significant signals are observed and the corresponding upper limits on the branching fractions at the 90% confidence level are determined to be B(J/psi -> D(0)e(+) e(-) + c.c.) < 8.5 x 10(-8) and B(psi(3686) -> D(0)e(+) e(-) + c.c.) < 1.4 x 10(-7), respectively. Our limit on B(J/psi -> D(0)e(+) e(-) + c.c.) is more stringent by 2 orders of magnitude than the previous results, and B(psi(3686) -> D(0)e(+) e(-) + c.c.) is measured for the first time.
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| 23. |
- Ablikim, M., et al.
(författare)
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Search for ψ(3686)→γηc(η(1405))→γπ+π−π0
- 2017
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Ingår i: Physical Review D. - 2470-0010 .- 2470-0029. ; 96:11
-
Tidskriftsartikel (refereegranskat)abstract
- Using a sample of 448.1×106 ψ(3686) events collected with the BESIII detector, a search for the isospin violating decay ηc→π+π−π0 via ψ(3686)→γηc is presented. No signal is observed, and the upper limit on B(ψ(3686)→γηc)×B(ηc→π+π−π0) is determined to be 1.6×10−6 at the 90% confidence level. In addition, a search for η(1405)→f0(980)π0 in ψ(3686) radiative decays is performed. No signal is observed, and the branching fraction B(ψ(3686)→γη(1405))×B(η(1405)→f0(980)π0)×B(f0(980)→π+π−) is calculated to be less than 5.0×10−7 at the 90% confidence level.
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| 24. |
- Ablikim, M., et al.
(författare)
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Study of two-photon decays of pseudoscalar mesons via J/Psi radiative decays
- 2018
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Ingår i: Physical Review D. - 2470-0010 .- 2470-0029. ; 97:7
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Tidskriftsartikel (refereegranskat)abstract
- Using a sample of 4.48 x 10(8) Psi(3686) events collected with the BESIII detector at the BEPCII collider, we study the two-photon decays of the pseudoscalar mesons pi(0,) eta , eta' , eta(1405), eta(1475), eta(1760), and X(1835) in J/Psi radiative decays using Psi(3686) -> pi(+) pi(-) J/Psi events. The pi(0), eta, and eta' mesons are clearly observed in the two-photon mass spectra, and the branching fractions are determined to be B(J/Psi -> gamma pi(0) -> 3 gamma) = (3.57 +/- 0.12 +/- 0.16) x 10(-5), B(J/Psi -> gamma eta -> 3 gamma) = (4.42 +/- 0.04 +/- 0.18) x 10(-4), and B(J/Psi -> gamma eta' -> 2 gamma) = (1.26 +/- 0.02 +/- 0.05) x 10(-4), where the first error is statistical and the second is systematic. No clear signal for eta(1405), eta(1475), eta(1760) or X(1835) is observed in the two- photon mass spectra, and upper limits at the 90% confidence level on the product branching fractions are obtained.
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| 25. |
- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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