1. |
- Bashari, Mohanad, et al.
(författare)
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Fabrication and Characterization of Dextranase Nano-Entrapped Enzymes in Polymeric Particles Using a Novel Ultrasonication-Microwave Approach
- 2023
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Ingår i: Catalysts. - : MDPI. - 2073-4344. ; 13:1
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Tidskriftsartikel (refereegranskat)abstract
- In the current study, a novel method to improve the nano-entrapment of enzymes into Ca-alginate gel was investigated to determine the synergistic effects of ultrasound combined with microwave shock (UMS). The effects of UMS treatment on dextranase enzymes loading effectiveness (LE) and immobilization yield (IY) were investigated. By using FT-IR spectra and SEM, the microstructure of the immobilized enzyme (IE) was characterized. Additionally, the free enzyme was used as a control to compare the reusability and enzyme-kinetics characteristics of IEs produced with and without UMS treatments. The results demonstrated that the highest LE and IY were obtained when the IE was produced with a US of 40 W at 25 kHz for 15 min combined with an MS of 60 W at a shock rate of 20 s/min for 20 min, increasing the LE and the IY by 97.32 and 78.25%, respectively, when compared with an immobilized enzyme prepared without UMS treatment. In comparison with the control, UMS treatment dramatically raised the Vmax, KM, catalytic, and specificity constant values for the IE. The outcomes suggested that a microwave shock and ultrasound combination would be an efficient way to improve the immobilization of enzymes in biopolymer gel.
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2. |
- Li, Jing, et al.
(författare)
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Neurotensin accelerates atherosclerosis and increases circulating levels of short-chain and saturated triglycerides
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Ingår i: Atherosclerosis. - 0021-9150.
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Tidskriftsartikel (refereegranskat)abstract
- Background and aims: Obesity and type 2 diabetes are significant risk factors for atherosclerotic cardiovascular disease (CVD) worldwide, but the underlying pathophysiological links are poorly understood. Neurotensin (NT), a 13-amino-acid hormone peptide, facilitates intestinal fat absorption and contributes to obesity in mice fed a high-fat diet. Elevated levels of pro-NT (a stable NT precursor produced in equimolar amounts relative to NT) are associated with obesity, type 2 diabetes, and CVD in humans. Whether NT is a causative factor in CVD is unknown. Methods: Nt+/+ and Nt–/– mice were either injected with adeno-associated virus encoding PCSK9 mutants or crossed with Ldlr–/– mice and fed a Western diet. Atherosclerotic plaques were analyzed by en face analysis, Oil Red O and CD68 staining. In humans, we evaluated the association between baseline pro-NT and growth of carotid bulb thickness after 16.4 years. Lipidomic profiles were analyzed. Results: Atherosclerotic plaque formation is attenuated in Nt-deficient mice through mechanisms that are independent of reductions in circulating cholesterol and triglycerides but associated with remodeling of the plasma triglyceride pool. An increasing plasma concentration of pro-NT predicts atherosclerotic events in coronary and cerebral arteries independent of all major traditional risk factors, indicating a strong link between NT and atherosclerosis. This plasma lipid profile analysis confirms the association of pro-NT with remodeling of the plasma triglyceride pool in atherosclerotic events. Conclusions: Our findings are the first to directly link NT to increased atherosclerosis and indicate the potential role for NT in preventive and therapeutic strategies for CVD.
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3. |
- Liu, Yong-feng, et al.
(författare)
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A turn-on ratiometric fluorescent nanoprobe based on AgInZnS and nitrogen-doped graphene quantum dots for Cd2+ detection in lake water
- 2022
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Ingår i: Journal of Materials Science. - : Springer Nature. - 0022-2461 .- 1573-4803. ; 57:36, s. 17336-17346
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Tidskriftsartikel (refereegranskat)abstract
- Excessive Cd2+ poses adverse influences on ecosystem and human beings, but its precise detection via a facile and environment-friendly method with resistance to interference is still a challenge. Here, a turn-on ratiometric fluorescent nanoprobe for Cd2+ detection is established using yellow-emission AgInZnS quantum dots (AIZS QDs) and blue-emission nitrogen-doped graphene quantum dots (NGQDs), which serve as a recognition unit and internal reference signal, respectively. Cd2+ could enhance the fluorescence of AIZS QDs due to the passivation of surface defects, while it has no significant effect on that of NGQDs. This nanoprobe has a large detection range from 0.5 to 100 µM and a limit of detection low to 28.6 nM. It shows strong anti-interference ability for Cd2+ even in lake water samples with recovery from 98 to 101% and low relative standard deviation of 1.01%, indicating its excellent effectuation to real-application world.
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4. |
- Liu, Yong-feng, et al.
(författare)
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Highly Soluble CsPbBr3 Perovskite Quantum Dots for Solution-Processed Light-Emission Devices
- 2021
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Ingår i: ACS Applied Nano Materials. - : American Chemical Society (ACS). - 2574-0970. ; , s. 1162-1174
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Tidskriftsartikel (refereegranskat)abstract
- We report on the synthesis of CsPbBr3 perovskite quantum dots (PeQDs) with a high solubility of 75 g/L in toluene and a good film-forming property, as enabled by a dense layer of didodecyldimethylammonium bromide and octanoic acid surface ligands. The crystalline and monodisperse PeQDs feature a cubic-like shape, with an edge length of 10.1 nm, and a high photoluminescence quantum yield of greater than 90% in toluene solution and 36% as a thin film. We find that the PeQDs are n-type doped following the synthesis but also that they can be p-type and additionally n-type doped by in situ electrochemistry. These combined properties render the PeQDs interesting for the emitter in solution-processed light-emitting electrochemical cells (LECs), and we report a PeQD-LEC with air-stabile electrodes that emits with a narrow emission spectrum (λpeak = 514 nm, full width at half-maximum = 24 nm) and a luminance of 250 cd/m2 at 4 V and a luminance of 1090 cd/m2 at 6.8 V. To reach this performance, it was critical to include a thin solution-processed layer comprising p-type poly(vinyl carbazole) and a tetrahexylammonium tetrafluoroborate ionic liquid between the PeQD emission layer and the anode in order to compensate for the as-synthesized n-type doping of the PeQDs.
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5. |
- Liu, Yong-feng, et al.
(författare)
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Sulfur quantum dot as a fluorescent nanoprobe for Fe3+ ions : Uncovering of detection mechanism, high sensitivity, and large detection range
- 2023
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Ingår i: Journal of Luminescence. - : Elsevier. - 0022-2313 .- 1872-7883. ; 257
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Tidskriftsartikel (refereegranskat)abstract
- Sulfur quantum dots (SQDs), as a novel metal-free fluorescent material, are getting increasingly tremendous attention in metal ion detection, especially for Fe3+, due to the merits of antimicrobial potential, low toxicity, and exciting optoelectronic properties. However, sensing mechanism of SQD based fluorescent probe for Fe3+ is not clear yet, and high sensitivity and large detection range remain a challenge. Here, we report the synthesis of hydrophilic SQDs as a fluorescent nanoprobe for Fe3+ via a fluorescent turn-off mode. We systematically studied the quenching mechanism by ultraviolet–visible absorption spectra, steady-state and time-resolved photoluminescent spectra, and temperature-dependent quenching constants. Results unclearly evidenced the quenching behavior to both inner filter effect and static quenching. Furthermore, the nanoprobe presents a large detection range from 2.5 to 700 μM and a limit of detection low to 53.6 nM, both of which are the record performance to our knowledge. At last, it shows high selectivity toward Fe3+ and presents no ionic strength effect in the range of investigation, which enables surprising results for Fe3+ detection in deionized water with interference ion and real water samples.
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6. |
- Wang, Chuan Fei, et al.
(författare)
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Thermal-annealing effects on energy level alignment at organic heterojunctions and corresponding voltage losses in all-polymer solar cells
- 2020
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Ingår i: Nano Energy. - : ELSEVIER. - 2211-2855 .- 2211-3282. ; 72
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Tidskriftsartikel (refereegranskat)abstract
- Matched energy level alignment is a key requirement for efficient organic devices such as organic light-emitting diodes, photovoltaics, and field-effect transistors. The effect of thermal stress/annealing on energy level alignment and related properties of the devices are less discussed compared to the extensively explored effect on morphology and corresponding device performance. Here all polymer solar cells (all-PSCs) are employed to study thermal annealing effects on energy level alignment and the corresponding effect on the device properties of the all-PSCs. It is found that optimized energy level alignment can be achieved by thermal annealing. An interface dipole layer at the donor/acceptor interface is introduced by energy level realignment that assists charge generation by reducing geminate recombination so that the voltage loss is dramatically reduced, improving the performance of the all-PSCs.
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7. |
- Wang, Jinpeng, et al.
(författare)
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Metabolic engineering for increased lipid accumulation in Yarrowia lipolytica – A Review
- 2020
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Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 313
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Forskningsöversikt (refereegranskat)abstract
- Current energy security and climate change policies encourage the development and utilization of bioenergy. Oleaginous yeasts provide a particularly attractive platform for the sustainable production of biofuels and industrial chemicals due to their ability to accumulate high amounts of lipids. In particular, microbial lipids in the form of triacylglycerides (TAGs) produced from renewable feedstocks have attracted considerable attention because they can be directly used in the production of biodiesel and oleochemicals analogous to petrochemicals. As an oleaginous yeast that is generally regarded as safe, Yarrowia lipolytica has been extensively studied, with large amounts of data on its lipid metabolism, genetic tools, and genome sequencing and annotation. In this review, we highlight the newest strategies for increasing lipid accumulation using metabolic engineering and summarize the research advances on the overaccumulation of lipids in Y. lipolytica. Finally, perspectives for future engineering approaches are proposed.
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8. |
- Wang, Qingqing, et al.
(författare)
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An organic memory phototransistor based on oxygen-assisted persistent photoconductivity
- 2022
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Ingår i: Organic electronics. - : Elsevier. - 1566-1199 .- 1878-5530. ; 100
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Tidskriftsartikel (refereegranskat)abstract
- Persistent photoconductivity (PPC) behavior in organic phototransistors has fascinating potentials in applications of photoelectronic devices. A key issue is how the presence of air affects the PPC behavior. Here, combining with the theoretical and experimental results, the PPC behavior is associated with photogenerated electrons trapped in oxygen atom-induced the reduced Lowest Unoccupied Molecular Orbitals or oxygen molecule-induced new trap state within energy bandgap of organic semiconductor. Inspired by the potential applications arising from the PPC behavior, organic memory phototransistors (OMPTs) are achieved by light programming and electrical erasing. The OMPTs show bistable current states as well as long retention times. Our results suggested that oxygen in air plays a key role in PPC behavior and provides a guidance for controlling the PPC behavior toward integrated multifunctional optoelectronic devices.
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9. |
- Wang, Qingqing, et al.
(författare)
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Anisotropic valence band dispersion of 2D molecular crystals of C6-DPA and its charge transport dependence
- 2023
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Ingår i: Materials Advances. - : ROYAL SOC CHEMISTRY. - 2633-5409. ; 4:9, s. 2201-2206
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Tidskriftsartikel (refereegranskat)abstract
- The unique properties and potential optoelectronic applications of two-dimensional molecular crystals (2DMCs) of organic semiconductors make them fascinating research subjects. With advancements in crystal engineering, it is becoming reality to produce 2DMCs with molecular-level thickness and large areas up to the centimeter scale, enabling us to directly explore the electronic structure of 2DMCs and to correlate them with their electrical properties. Here, we investigated the electronic structure of 2DMCs of C6-DPA using photoemission spectroscopy and electrical properties based on organic field-effect transistors. Our findings indicate that anisotropic band dispersion is present in the ab plane of the 2DMCs of C6-DPA which is in good agreement with the in-plane anisotropic mobility, i.e., the direction of the strongest molecular overlap coincides with the direction of the highest mobility.
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