| 1. |
- Xu, Changdan, et al.
(författare)
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CTCs Detection and Whole-exome Sequencing Might Be Used to Differentiate Benign and Malignant Pulmonary Nodules
- 2023
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Ingår i: Chinese Journal of Lung Cancer. - 1009-3419. ; 26:6, s. 449-460
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Tidskriftsartikel (refereegranskat)abstract
- Background and objective Low-density computed tomography (LDCT) improved early lung cancer diagnosis but introduces an excess of false-positive pulmonary nodules data. Hence, accurate diagnosis of early-stage lung cancer remains challenging. The purpose of the study was to assess the feasibility of using circulating tumour cells (CTCs) to differentiate malignant from benign pulmonary nodules. Materials and methods 122 patients with suspected malignant pulmonary nodules detected on chest CT in preparation for surgery were prospectively recruited. Peripheral blood samples were collected before surgery, and CTCs were identified upon isolation by size of epithelial tumour cells and morphological analysis. Laser capture microdissection, MALBAC amplification, and whole-exome sequencing were performed on 8 samples. The diagnostic efficacy of CTCs counting, and the genomic variation profile of benign and malignant CTCs samples were analysed. Results Using 2.5 cells/5 mL as the cut-off value, the area under the receiver operating characteristic curve was of 0.651 (95% confidence interval: 0.538-0.764), with a sensitivity and specificity of 0.526 and 0.800, respectively, and positive and negative predictive values of 91.1% and 30.3%, respectively. Distinct sequence variations differences in DNA damage repair-related and driver genes were observed in benign and malignant samples. TP53 mutations were identified in CTCs of four malignant cases; in particular, g.7578115T>C, g.7578645C>T, and g.7579472G>C were exclusively detected in all four malignant samples. Conclusion CTCs play an ancillary role in the diagnosis of pulmonary nodules. TP53 mutations in CTCs might be used to identify benign and malignant pulmonary nodules.
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| 2. |
- Yang, Hui, et al.
(författare)
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Growth Factor Changes in Cerebrospinal Fluid of Children with Mental Retardation before and after Neural Precursor Cell Transplantation
- 2018
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Ingår i: CNS & Neurological Disorders. - : BENTHAM SCIENCE PUBL. - 1871-5273 .- 1996-3181. ; 17:2, s. 98-105
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Tidskriftsartikel (refereegranskat)abstract
- Objective: To investigate growth factor changes in cerebrospinal fluid (CSF) of children with mental retardation (MR) before and after neural precursor cell transplantation (NPCT), in an attempt to provide experimental support for the clinical treatment of MR with NPCT.Methods: The study comprised of 28 MR children who received twice NPCT in our hospital. CSF was collected at both times of NPCT to assess growth factors by ELISA. In addition, the content of insulin-like growth factor 1 (IGF-1) in CSF was assayed to determine possible correlations between IGF-1 changes and the short-term therapeutic effect of NPCT.Results: Of all the growth factors detected in CSF, only IGF-1 was increased significantly after NPCT (P<0.05). Fifteen of the twenty-eight MR children achieved short-term therapeutic efficacy, whereby the content of IGF-1 after NPCT was significantly higher than that before NPCT (P<0.05). There was no difference in IGF-1 content before and after NPCT in the remaining 13 MR children without short-term therapeutic effect (P=0.657). There was a significant difference in IGF-change between the two groups of patients (P<0.05).Conclusion: IGF-1 may be one of the mechanisms contributing to the therapeutic effect of NPCT.
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| 3. |
- Chen, Qingguo, et al.
(författare)
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Preparation and properties of Ce0.5Nd0.5PO4 monazite ceramics by pressureless sintering and spark plasma sintering (SPS)
- 2024
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Ingår i: Ceramics International. - : Elsevier. - 0272-8842 .- 1873-3956. ; 50:12, s. 20803-20810
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Tidskriftsartikel (refereegranskat)abstract
- LnPO4·nH2O rhabdophane/monazite is an important actinide-bearing material in the back end of the nuclear fuel cycle. In this work, using Nd3+ to simulate trivalent actinides, Ce0.5Nd0.5PO4·0.667H2O rhabdophane was synthesized by the wet-chemistry method in 90 °C and pH = 1 solution. The sintering behavior of associated Ce0.5Nd0.5PO4 monazite ceramics was investigated by pressureless sintering and spark plasma sintering (SPS), along with appraising their chemical stability. The results reveal that Ce3+ is successfully replaced by Nd3+ in the rhabdophane lattice, and rhabdophane can transform to monazite with a temperature over 750 °C. SPS can compact the ceramic sample at a lower temperature compared to pressureless sintering even if the grain size is smaller. Adding a pre-sintering process of 900 °C before SPS and pressureless sintering will further improve the density and Vickers hardness of ceramics. For the chemical stability of Ce0.5Nd0.5PO4 monazite ceramics, Ce is more likely to be leached compared to Nd, and the ceramics prepared by SPS with a pre-sintering process have the lowest normalized leaching rates, associated with the values of 2.03 × 10−5 g·m−2·d−1 and 0.75 × 10−5 g·m−2·d−1 for Ce and Nd, respectively.
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| 4. |
- Qu, Suqing, et al.
(författare)
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Clinical Observation of Electroencephalographic Changes and Risk of Convulsion Occurrence in Children Receiving Neural Precursor Cell Transplantation
- 2018
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Ingår i: CNS & Neurological Disorders. - : Bentham Science Publishers Ltd.. - 1871-5273 .- 1996-3181. ; 17:3, s. 233-239
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: This study was intended to observe electroencephalographic (EEG) changes and convulsion attacks in children receiving neural precursor cell transplantation, and to explore the possibility of electrophysiological changes and risk of convulsion occurrence after cell transplantation.Method: 228 children were included in this study who received neural precursor cell transplantation in our hospital between March 2008 and July 2012. No history of convulsion attacks was elicited before cell transplantation. Data about EEG change and convulsion occurrence before and after cell transplantation were analyzed statistically.Results: Of the 228 pediatric patients, EEG improvement, deterioration and no significant change were observed in 60, 45 and 122 patients, respectively. One month after transplantation, four (1.76%) patients experienced new convulsions. Of the 227 patients, 25 showed increased and/or abnormal discharges on EEG. Of these, 19 underwent EEG re-examination six months post-operation. Except the convulsive cases mentioned above, there were no new cases of convulsions in the remaining patients. Of the 27 patients including those with abnormal discharge, increased discharge and convulsion attacks, 17 achieved varying degrees of therapeutic efficacy.Conclusion: Intraventricular transplantation of neural precursor cells is associated with EEG changes in some children and clinical convulsion attacks in individual patients. However, these abnormal changes do not last long and usually return to normal levels within 1-6 months after surgery, along with disappearance of convulsions. Simultaneous occurrence of EEG changes and convulsions do not appear to affect therapeutic efficacy.
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| 5. |
- Zhao, Xiaofeng, et al.
(författare)
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Incorporation of Th4+ and Sr2+ into Rhabdophane/Monazite by Wet Chemistry : Structure and Phase Stability
- 2023
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Ingår i: Inorganic Chemistry. - : American Chemical Society (ACS). - 0020-1669 .- 1520-510X. ; 62:38, s. 15605-15615
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Tidskriftsartikel (refereegranskat)abstract
- Rhabdophane is an important permeable reactive barrier to enrich radionuclides from groundwater and has been envisaged to host radionuclides in the backend of the nuclear fuel cycle. However, understanding of how An4+ and Sr2+ precipitate into rhabdophane by wet chemistry has not been resolved. In this work, Th4+ and Sr2+ incorporation in the rhabdophane/ monazite structure as La1-2xSrxThxPO4.nH2O solid solutions is successfully achieved in the acid solution at 90 degrees C. Some specific issues such as lattice occupation of Th4+ and Sr2+, precipitation reaction kinetics, and crystal growth affected by starting stoichiometry are discussed in detail, along with investigating the chemical stability of La1-2xSrxThxPO4.nH2O precipitations and associated La(1-2x)Sr(x)ThxPO(4) monazite. The results reveal that the excess of Sr2+ appears to be a prevailing factor with a suggested initial Sr: Th = 2 to obtain the stability domain of La1-2xSrxThxPO4.nH(2)O (x = 0 similar to 0.1). A rapid ion removal associated with a nucleation process has been observed within 8 h, and Th4+ can be removed more than 98% after 24 h in 0.01 mol/L solutions. From structural energetics based on density functional theory, the lattice occupation of Th4+ and Sr2+ is energetically favorable in nonhydrated lattice sites of [LaO8], although two-thirds of lattice sites are associated with [LaO8.H2O] hydrated sites. Intriguingly, the crystal transformation from rhabdophane to monazite associated with the transformation from [SrO8] to [SrO9] polyhedra can greatly improve the leaching stability of Sr2+.
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