| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Yen, Ying-Tzu, et al.
(författare)
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Prominent Enhancement of Cisplatin Efficacy with Optimized Methoxy Poly(ethylene glycol)-Polycaprolactone Block Copolymeric Nanoparticles
- 2020
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Ingår i: Journal of Biomedical Nanotechnology. - : AMER SCIENTIFIC PUBLISHERS. - 1550-7033 .- 1550-7041. ; 16:3, s. 335-343
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Tidskriftsartikel (refereegranskat)abstract
- Chemotherapy has been one of the major standard treatments for a variety of cancers. cis-Dichlorodiamminoplatiunum(II) (cisplatin, CDDP), as one of the anticancer agents, demonstrated excellent efficacy against tumor and has been an indispensable component in chemotherapy, chemoradiation, chemo-molecular targeted therapy and chemo-immunotherapy. However, its therapeutic concentration was limited since its inevitable toxicity. Previously, we have constructed CDDP-loaded nanoparticles (NPs) with mixture of poly(ethyleneglycol)-polycaprolactone (PEG-PCL) and polycarprolactone (HO-PCL) by a facile method. The most optimal proportion of the two copolymers was selected through a series of physical, chemical, cytological and histological evaluations. In the present study, we explored the mechanisms of NPs and observed the in vivo antitumor effect after administrating CDDP-loaded PEG-PCL NPs. Positron emission tomography as well as computed tomography (PET/CT) were adopted for detecting tumoral metabolic activity. Images from fluorescence microscope revealed superior cellular uptake of CDDP-loaded NPs with rhodamine B aggregated intracellularly in cancer cells. Similar apoptotic rates between free CDDP group and CDDP-loaded NPs group was measured by flow cytometry. Tumor volumes and murine weights confirmed the superiority of CDDP-loaded NPs in therapeutic efficacy as compared with free CDDP. Blood tests showed milder side effects in CDDP-loaded nanoparticle group. PET/CT images illustrated less uptake intensity of FDG in mice received CDDP-loaded NPs than free CDDP. Our results suggest that PEG-PCL/PCL NPs could be a promising antitumor drug carrier for CDDP delivery with solid efficacy and minor side effects.
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| 3. |
- Bao, Zijia, et al.
(författare)
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A helical polypyrrole nanotube interwoven zeolitic imidazolate framework and its derivative as an oxygen electrocatalyst
- 2022
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Ingår i: Chemical Communications. - : Royal Society of Chemistry (RSC). - 1359-7345 .- 1364-548X. ; 58:80, s. 11288-11291
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Tidskriftsartikel (refereegranskat)abstract
- A helical polypyrrole nanotube interwoven zeolitic imidazolate framework (ZIF) has been prepared for the first time. After pyrolysis, the helical carbon could act as highly active sites, while the 3D-connected nanoarchitecture contributed to fast charge transfer. The derived carbon material exhibits high activity for the ORR and good performance for a Zn–air battery.
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| 4. |
- Liu, Fangcen, et al.
(författare)
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Biocompatible Nanoparticles as a Platform for Enhancing Antitumor Efficacy of Cisplatin-Tetradrine Combination
- 2021
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Ingår i: Nanoscale Research Letters. - : Springer. - 1931-7573 .- 1556-276X. ; 16:1
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Tidskriftsartikel (refereegranskat)abstract
- Combination therapy has been a standard strategy in the clinical tumor treatment. We have demonstrated that combination of Tetradrine (Tet) and Cisplatin (CDDP) presented a marked synergistic anticancer activity, but inevitable side effects limit their therapeutic concentration. Considering the different physicochemical and pharmacokinetic properties of the two drugs, we loaded them into a nanovehicle together by the improved double emulsion method. The nanoparticles (NPs) were prepared from the mixture of poly(ethyleneglycol)-polycaprolactone (PEG-PCL) and polycarprolactone (HO-PCL), so CDDP and Tet can be located into the NPs simultaneously, resulting in low interfering effect and high stability. Images from fluorescence microscope revealed the cellular uptake of both hydrophilic and hydrophobic agents delivered by the NPs. In vitro studies on different tumor cell lines and tumor tissue revealed increased tumor inhibition and apoptosis rates. As to the in vivo studies, superior antitumor efficacy and reduced side effects were observed in the NPs group. Furthermore, (18)FDG-PET/CT imaging demonstrated that NPs reduced metabolic activities of tumors more prominently. Our results suggest that PEG-PCL block copolymeric NPs could be a promising carrier for combined chemotherapy with solid efficacy and minor side effects.
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| 5. |
- Wang, Xinyue, et al.
(författare)
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Charge-Separated States Determined Photoinduced Electron Transfer Efficiency in a D-D-A System in an External Electric Field
- 2023
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Ingår i: Journal of Physical Chemistry C. - : American Chemical Society (ACS). - 1932-7447 .- 1932-7455.
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Tidskriftsartikel (refereegranskat)abstract
- Focusing on the photoinduced electron transfer properties of the D-D-A molecule ((TPA-TT)-BODIPY-C60) in an external electric field (Fext), the excited-state properties in which the double-donor molecule is excited to form three charge-separated states were simulated. The charge-Transfer processes of these three charge-separated states were investigated by considering the two donors as a whole ((TPA-TT-BODIPY)·+-C60·-) as a comparison object. The electronic coupling (VDA), reorganization energy (λ), and free energy (δG) of the different charge-separated states in Fext were calculated and simulated. The calculated results show that the λ of (TPA-TT-BODIPY)·+-C60·- ranges from 0.576 to 0.51 eV, and the calculated δG of exciton dissociation ranges from-1.402 to-1.143 eV, indicating that exciton dissociation occurs in the Marcus inverted region. In the range of Fext =-10 × 10-5 to 10 × 10-5 au, the trend of the charge-Transfer rate is gradually increasing, and the rate increase is mainly from the VDA and δG changes. Moreover, the rapid formation of the (TPA-TT)-BODIPY·+-C60·- charge-separated state and the formation of the long-lived (TPA-TT)·+-BODIPY-C60·- are indicated by the exciton dissociation rate. By studying the charge-Transfer parameters under different electric field directions, it is found that the regulation of electric field strength on the charge-Transfer rate is consistent. These results provide a feasible method for the rational design of a new type of electron transfer process with high efficiency of the D-D-A system.
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| 6. |
- Wang, Xinyue, et al.
(författare)
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External electric field-dependent photoinduced charge transfer in non-fullerene organic solar cells
- 2023
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Ingår i: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. - : Elsevier BV. - 1386-1425. ; 284
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Tidskriftsartikel (refereegranskat)abstract
- Based on Marcus theory, the photoinduced electron transfer properties of D-A type non-fullerene acceptor organic solar cells (OSCs) under the dependence of external electric field (Fext) were investigated. The research results shown that the charge transfer mode under different Fext intensities changes with certain regularity. Focusing on the important parameters (ΔG, λ, and VDA) that affect the charge transfer rate, it was found that both charge separation (|ΔGCS|>λ(1.3019 vs 0.8275 eV at Fext = 0) and charge recombiation (|ΔGCR|>λ, (1.9633 vs 0.8275 eV)) processes occur in the Marcus inverted region. The ΔGCS is relatively sensitive to Fext, and the calculated ΔGCS at different Fext intensities yields an increment of 0.0073 eV, which is also the main reason for the increase in the rate of charge separation. The ΔGCR ranges between −1.9633 and −1.9637 eV, is insensitive to Fext, and ΔGCR is significantly smaller than ΔGCS, which makes the charge recombination rate significantly smaller than the charge separation rate. For VDA, it is found that VDA will transition to a new level only when the Fext intensity reaches a certain intensity, which also enables to obtain a faster charge separation rate. By studying the charge transfer parameters in different polar solvents, it is found that polar solvents can indeed increase the charge transfer rate. To a certain extent, our results also demonstrate that the addition of Fext can further improve the performance of non-fullerene acceptor OSCs.
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| 7. |
- Cui, Xinyue, et al.
(författare)
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Esthetic appearances of anatomic contour zirconia crowns made by additive wet deposition and subtractive dry milling : A self-controlled clinical trial
- 2020
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Ingår i: The Journal of prosthetic dentistry (Print). - : Elsevier BV. - 0022-3913 .- 1097-6841. ; 123:3, s. 442-448
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Tidskriftsartikel (refereegranskat)abstract
- Statement of problem. Anatomic contour zirconia crowns are widely used in clinical dental practice because of their mechanical reliability and improved appearance. However, few studies have performed clinical evaluations of the esthetics of these crowns in terms of color and translucency gradient.Purpose. The purpose of this clinical trial was to compare the esthetic effect and color-matching behaviors of anatomic contour zirconia crowns manufactured with 3-dimensional (3D) gel deposition and dry milling methods.Material and methods. Twenty-seven premolar teeth of 27 participants received 2 identical anatomic contour zirconia crowns fabricated by additive 3D gel deposition or dry milling. Color differences (Delta E) between the crown and natural control teeth were measured by a dental shadematching device. Subjective color matching was rated by professionals using an extended visual rating scale for appearance match (EVRSAM) and by participants using a visual analog scale (VAS). Data were analyzed by using repeated measures ANOVA, the Bonferroni test, paired Student t test, Pearson chi-square test, and Wilcoxon test (alpha=.05).Results. Significant differences were found in SE between zirconia crown and core types (P<.05); however, there was no significant interaction between these factors (P>.05). The average SE of crowns made by wet deposition and dry milling were 2.45 +/- 1.60 and 4.55 +/- 1.54 (P<.05). The mean crown Delta E was significantly higher if a gold cast post-and-core was placed rather than a prefabricated fiber post and composite core (P<.05). Consistent with these findings, subjective color matching was significantly higher in the wet deposition group than in the dry milling group as rated by EVRSAM and VAS (P<.05).Conclusion. Anatomic contour zirconia crowns fabricated by additive wet deposition were better matched to adjacent teeth and had excellent esthetics in terms of color and translucency gradient.
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| 8. |
- Guo, Huijie, et al.
(författare)
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Regulation of photoinduced charge transfer in all-small-molecule organic solar cells through the synergistic effect of external electric field and solvent
- 2024
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Ingår i: Journal of Luminescence. - 0022-2313. ; 268
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Tidskriftsartikel (refereegranskat)abstract
- The donor (BTR-Cl) and acceptor (BTP-FCl-FCl) have well-defined small molecule properties and excellent repeatability, and they can form charge transfer complexes with a wide spectral absorption range. Using density functional theory (DFT), we simulate the photoinduced charge transfer of bulk-heterojunction (BHJ) organic solar cell (OSC) materials modulated by the external electric field (Fext) at the microscopic level. The excited-state properties, reorganization energy (λ), Gibbs free energy (ΔG), electron coupling matrix elements (VDA) and intermolecular charge transfer (ICT) rate dependent on Fext are systematically analyzed. The results manifest that Fext has apparent positive regulation on the charge separation rate (KCS), and VDA is the main factor affecting KCS. The synergistic effect of solvent and Fext on charge transfer process is further investigated. It is found that the charge transfer rate in solvent is lower than that in solvent-free conditions, and the charge transfer rate in chlorobenzene (CB) solvent does not change significantly under the control of Fext. At the same time, Fext has a positive effect on the charge transfer rate in tetrahydrofuran (THF) solvent. Considering the charge transfer rate under different solvents and Fext intensities, it is found that the solvent and Fext play an essential role in determining the KCS and charge recombination rate (KCR).
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| 9. |
- Kozielski, Frank, et al.
(författare)
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Identification of fragments binding to SARS-CoV-2 nsp10 reveals ligand-binding sites in conserved interfaces between nsp10 and nsp14/nsp16
- 2021
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Ingår i: RSC Chemical Biology. - 2633-0679. ; 3:1, s. 44-55
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Tidskriftsartikel (refereegranskat)abstract
- Since the emergence of SARS-CoV-2 in 2019, Covid-19 has developed into a serious threat to our health, social and economic systems. Although vaccines have been developed in a tour-de-force and are now increasingly available, repurposing of existing drugs has been less successful. There is a clear need to develop new drugs against SARS-CoV-2 that can also be used against future coronavirus infections. Non-structural protein 10 (nsp10) is a conserved stimulator of two enzymes crucial for viral replication, nsp14 and nsp16, exhibiting exoribonuclease and methyltransferase activities. Interfering with RNA proofreading or RNA cap formation represents intervention strategies to inhibit replication. We applied fragment-based screening using nano differential scanning fluorometry and X-ray crystallography to identify ligands targeting SARS-CoV-2 nsp10. We identified four fragments located in two distinct sites: one can be modelled to where it would be located in the nsp14–nsp10 complex interface and the other in the nsp16–nsp10 complex interface. Microscale thermophoresis (MST) experiments were used to quantify fragment affinities for nsp10. Additionally, we showed by MST that the interaction by nsp14 and 10 is weak and thereby that complex formation could be disrupted by small molecules. The fragments will serve as starting points for the development of more potent analogues using fragment growing techniques and structure-based drug design.
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| 10. |
- Shao, Yu, et al.
(författare)
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Feasibility of Using Floor Vibration to Detect Human Falls
- 2021
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Ingår i: International Journal of Environmental Research and Public Health. - : MDPI AG. - 1661-7827 .- 1660-4601. ; 18:1
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Tidskriftsartikel (refereegranskat)abstract
- With the increasing aging population in modern society, falls as well as fall-induced injuries in elderly people become one of the major public health problems. This study proposes a classification framework that uses floor vibrations to detect fall events as well as distinguish different fall postures. A scaled 3D-printed model with twelve fully adjustable joints that can simulate human body movement was built to generate human fall data. The mass proportion of a human body takes was carefully studied and was reflected in the model. Object drops, human falling tests were carried out and the vibration signature generated in the floor was recorded for analyses. Machine learning algorithms including K-means algorithm and K nearest neighbor algorithm were introduced in the classification process. Three classifiers (human walking versus human fall, human fall versus object drop, human falls from different postures) were developed in this study. Results showed that the three proposed classifiers can achieve the accuracy of 100, 85, and 91%. This paper developed a framework of using floor vibration to build the pattern recognition system in detecting human falls based on a machine learning approach.
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| 11. |
- Sun, Tao, et al.
(författare)
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Empirical evidence that manganese enrichment accelerates decomposition
- 2021
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Ingår i: Agriculture, Ecosystems & Environment. Applied Soil Ecology. - : Elsevier. - 0929-1393 .- 1873-0272. ; 168
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Tidskriftsartikel (refereegranskat)abstract
- Our understanding of the controls regulating the rate of litter decomposition is important for improving confidence in the parameterization of carbon cycle–climate feedbacks. Traditional conceptual models rely primarily on climate and lignin/N ratios as the main regulators of decomposition. Here we studied the effects of manganese (Mn) addition on long-term decomposition across 18 substrates in a laboratory incubation. Mn addition remarkably promoted later stage of decomposition, resulting into a smaller fraction of slowly decomposing litter. This dynamic is closely associated with the changes of activities of manganese peroxidase, an important enzyme with greater capacity for lignin degradation. Our findings suggest the necessity of incorporating the interaction of Mn and decomposition into biogeochemical models.
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| 12. |
- Tao, Yongqing, et al.
(författare)
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The application potential of self-glazed zirconia crowns confirmed by easy grinding and polishing of the enamel-like surface
- 2020
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Ingår i: Advances in Applied Ceramics. - : Informa UK Limited. - 1743-6753 .- 1743-6761. ; 119:5-6, s. 297-304
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Tidskriftsartikel (refereegranskat)abstract
- The surface profile roughness (Ra), surface area roughness (Sa), and surface topography of newly developed as-prepared, ground, and polished self-glazed zirconia (SGZ) were evaluated using a profilometer, 3D optical surface profiler, and SEM, with conventional dry-milled zirconia (CZ) as a reference. A statistical analysis was conducted using repeated measures analysis of variance (ANOVA) and the Bonferroni test (alpha = .05). Results revealed that the material (p = .005), clinical adjustment procedure (p < .001), and interaction between factors (p < .001) had statistically significant effects on the Ra values. SGZ showed lower Sa values than CZ during the same period. Ten patient cases involving the restoration of the monolithic anatomic contour SGZ crowns were investigated. All crowns remained functional until the latest clinical follow-up and no further antagonist wear was observed. Thus, SGZ had relatively lower surface roughness, which was also more easily altered than that of CZ.
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| 13. |
- Wang, Xinyue, 1995, et al.
(författare)
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A Pathway to Climate Neutral Buildings：Definitions, Policy and Stakeholder Understanding in Sweden and China
- 2022
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Ingår i: IOP Conference Series: Earth and Environmental Science. - : IOP Publishing. - 1755-1307 .- 1755-1315. ; 1078:1
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Konferensbidrag (refereegranskat)abstract
- In recent years, 'climate neutral buildings' has become one of the most popular emerging terms in the context of global warming and the built environment. However, due to a vague definition, the term still lacks real-world uptake in practice. While initial research focuses on 'climate neutral buildings', few have discussed this term from the perspective of different countries or stakeholders. To address this gap, this paper explores the current understanding and future development of the term 'climate neutral buildings' in Sweden and China. Through a literature review of related definitions, an investigation of current regulations, and stakeholder interviews in both countries, we find that Sweden and China are in different stages of development towards climate neutral buildings. Sweden seems to surpass China in terms of theoretical research, regulation development and stakeholder understanding. Despite this, the two countries share similar issues regarding the future development of climate neutral buildings. Both countries lack an official interpretation of 'climate neutral buildings', sufficient regulations, and collaborations among different stakeholders. This paper suggests a foundation for the future development of climate neutral buildings.
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| 14. |
- Wang, Xinyue, et al.
(författare)
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Exploring the effect of electric field on charge-transfer states at non-fullerene D/A interface
- 2023
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Ingår i: Journal of Molecular Liquids. - 0167-7322. ; 390
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Tidskriftsartikel (refereegranskat)abstract
- Using Marcus theory, the electric-field-dependent charge-separation dynamics of non-fullerene acceptors D/A heterojunctions are simulated. On the D/A interface, the excited-state characteristics show obvious differences under different electric field intensities, providing microscopic details of the non-fullerene D/A interface at the atomic level. For different electric field conditions, so the calculated reorganization energy ranges from 0.97 to 1 eV. By comparing other charge transfer parameters of this system, it was found that the reorganization energy mainly determines the charge-transfer rate. The results show that the charge transfer rate does exhibit a variation that depends on the electric field intensity and is consistent with the variation in the Marcus inverted region. By evaluating the rate of charge separation/recombination, it is judged that its charge separation shows significant advantages, and the trifurcated structure of the molecule also provides multiple charge transfer paths for charge separation, which contributes to the charge generation mechanism.
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| 15. |
- Wang, Xinyue, 1995, et al.
(författare)
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Identifying influential architectural design variables for early-stage building sustainability optimization
- 2024
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Ingår i: Building and Environment. - 0360-1323. ; 252
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Tidskriftsartikel (refereegranskat)abstract
- Architectural design variables (ADVs) highly influence a building's sustainability performance. Thus, identifying which ADVs are most influential in a building's early stages is of great significance, especially when using computational building design optimization tools. Currently, sensitivity analysis based on computer simulations is the most commonly used means to identify which ADVs are the most influential in the early stages. However, we suggest that a stakeholder perspective should also be considered as stakeholders possess domain-specific knowledge and expertise as well as a contextual understanding that can greatly enhance the development and deployment of building design optimization tools. To explore the above, we combined a literature review with survey data from 24 architects and sustainability consultants in the Nordics. Surprisingly, we found that the influential ADVs in the literature do not always align with those of our surveyed stakeholders. For example, we found that the literature considers building plan, window-to-wall-ratio (WWR), and wall material as the most influential ADVs, which contrasts with storey number, storey height, WWR, roof material and wall material considered by stakeholders to be the most influential. We also found that the most influential ADVs differ across different sustainability optimization objectives, and that these also differ from the literature. Despite our limited survey sample, our study provides insights into influential ADVs and as such has implications for the development, use, and performance of computational building design optimization tools.
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| 16. |
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| 17. |
- Wang, Xinyue, 1995
(författare)
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Towards Machine Learning Application in Early-Stage Building Energy Optimization
- 2024
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- As more than one-third of global greenhouse gas emissions are related to the operation of buildings, reducing building energy demand is a key area in the architecture, engineering, and construction (AEC) industry. One promising means is to conduct early-stage building energy optimization. Optimizing the influential architectural design variables (ADVs) in the early stage can significantly reduce building energy demand efficiently. However, current optimization tools often use simulation engines to calculate energy demand for various design alternatives, which can be very time-consuming. To improve the speed of the optimization tools, machine learning (ML) energy prediction models are introduced to replace the energy simulation engines. An ML prediction model requires three crucial elements: the input ADVs, the dataset containing both ADVs and the energy demand of various building design configurations, and the ML algorithm. This thesis investigates how to identify the above elements and provides recommendations for researchers and tool developers at the end. The input ADVs are identified through a combination of a literature review and a stakeholder survey. Results indicate that building plan, window-to-wall ratio (WWR) on four facades, and wall material are considered influential by both the literature and stakeholders. Building plan, building orientation, shading device, storey number, storey height, roof type, and roof material are also considered influential by stakeholders. It is suggested that insights from both perspectives should be included when developing the ML prediction model. The best-performing ML algorithm, as well as the characteristics of the corresponding synthetic dataset, are identified through multiple ML experiments. A parametric model is developed to generate multiple synthetic datasets with different sizes and different building types, referred to as diversity. Five ML algorithms are selected through a literature review. By conducting ML experiments with different combinations of datasets and ML algorithms, recommendations are provided regarding which algorithms to employ with what type of dataset. Support Vector Model (SVM) performs best in general. Multiple Linear Regression (MLR) performs well with small and low-diverse datasets while Artificial Neural Network (ANN) performs well with large and high-diverse datasets. When generating synthetic training datasets, it is recommended that the dataset needs to have more than 1440 data points and a diversity that covers around 67% of the diversity in the testing dataset to achieve reasonable accuracy. This thesis explored how ML can support early-stage building energy optimization by investigating how to develop an ML energy prediction model and providing recommendations. This study offers a more holistic point of view in developing ML-based tools by incorporating stakeholders’ opinions in selecting input ADVs. The findings fill the gap of the lack of understanding in the compatibility between different ML algorithms and synthetic datasets in the development of ML models for building energy prediction. In general, this study offers insights for researchers and tool developers in developing ML building energy prediction models and contributes to the application of artificial intelligence in the AEC industry and a more sustainable built environment.
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| 18. |
- Yu, Bowei, et al.
(författare)
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Age-specific and sex-specific associations of visceral adipose tissue mass and fat-to-muscle mass ratio with risk of mortality
- 2023
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Ingår i: Journal of Cachexia, Sarcopenia and Muscle. - : John Wiley & Sons. - 2190-5991 .- 2190-6009. ; 14:1, s. 406-417
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Tidskriftsartikel (refereegranskat)abstract
- BackgroundLimited studies have explored the association between visceral adipose tissue (VAT) mass and fat-to-muscle mass ratio (FMR) and mortality. We aimed to evaluate the sex-specific association of VAT and FMR with all-cause and cause-specific mortality by age. MethodsA total of 438 896 participants (49.8% men, mean age +/- standard deviation: 57 +/- 8 years for men; 56 +/- 8 years for women) were included from the UK Biobank cohort. The nature of VAT was predictive, as obtained by sex-stratified, non-linear prediction models. Fat and muscle mass were estimated using a bioelectrical impedance assessment device. FMR was calculated as the fat mass divided by the muscle mass in the whole body. VAT and FMRs were divided into quintiles in ascending order, and the 3rd quintile was used as the reference. Cox regression analyses were used to estimate the associations between VAT, FMR and mortality. ResultsDuring a median of 12.4 years of follow-up, we documented 29 903 deaths. After adjusting for various covariates, the individuals in the highest quintiles of VAT and FMR had the highest hazard ratios (HRs) of all-cause mortality [1.24 (95% confidence interval: 1.17-1.33) for VAT and 1.24 (1.17-1.31) for FMR in men; and 1.11 (1.03-1.21) for VAT in women], except that the 1st quintile of FMR in women had the greatest HR [1.18 (1.09-1.27)]. Significant interactions were observed in both sexes according to age category (P for interaction < 0.05). Among men <50 years, participants in the 1st and 5th quintiles of VAT and FMR had significantly higher risks of mortality [1.30 (1.02-1.66) and 1.67 (1.27-2.19) in VAT; 1.25 (0.99-1.56) and 1.41 (1.11-1.79) in FMR, respectively]; in women, this phenomenon was observed in the >= 60 age group [1.16 (1.06-1.27) and 1.19 (1.08-1.31) in VAT; 1.18 (1.08-1.29) and 1.11 (1.01-1.22) in FMR, respectively]. VAT showed a linear positive association with mortality in women <60 years and a J-shaped association from respiratory disease in both sexes >= 60 years. FMR showed a linear positive association with mortality from cancer in men <60 years and a J-shaped association with mortality from cause-specific mortality in both sexes >= 60 years, except for mortality from cardiovascular disease in men. ConclusionsMost associations of VAT and FMR with all-cause mortality were J-shaped and were significantly modified by age status (<50, 50-59 and >= 60 years). The clinical implication is that regarding body composition and VAT mass, different health strategies may be adopted for people of different sexes and ages.
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| 19. |
- Zhao, Yadong, 1985-, et al.
(författare)
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Collagens for surimi gel fortification : Type-dependent effects and the difference between type I and type II
- 2023
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Ingår i: Food Chemistry. - : Elsevier BV. - 0308-8146 .- 1873-7072. ; 407, s. 135157-
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Tidskriftsartikel (refereegranskat)abstract
- Surimi products have unsatisfactory gel properties. Hence, this study evaluates the effect of collagen-adding on surimi gel properties and provides the first observation results regarding collagen type influence. With higher water solubility and more charged amino acids than type II, collagen type I intertwines with surimi myofibrillar proteins better to induce higher exposure of protein functional domains, more sufficient conformational changes of myosin and greater formation of chemical forces among proteins. These enhancements accelerate the gelation rate, leading to a well-stabilized surimi gel. The collagen I-containing surimi gels show more compact structures with uniformly distributed smaller pores than those containing collagen II, thereby providing the final products with higher water holding capacity and better textural profiles. As such, the surimi gel fortification performance of collagen I and the well-elucidated collagen-myofibrillar protein interaction mechanism will guide the further exploitation of collagen as an effective additive in the food industry.
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