| 1. |
- Cossarizza, A., et al.
(author)
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
- 2019
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In: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 49:10, s. 1457-1973
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Journal article (peer-reviewed)abstract
- These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
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| 2. |
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- 2019
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Journal article (peer-reviewed)
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| 3. |
- Sandrock, I, et al.
(author)
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Genetic models reveal origin, persistence and non-redundant functions of IL-17-producing γδ T cells
- 2018
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In: The Journal of experimental medicine. - : Rockefeller University Press. - 1540-9538 .- 0022-1007. ; 215:12, s. 3006-3018
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Journal article (peer-reviewed)abstract
- γδ T cells are highly conserved in jawed vertebrates, suggesting an essential role in the immune system. However, γδ T cell–deficient Tcrd−/− mice display surprisingly mild phenotypes. We hypothesized that the lack of γδ T cells in constitutive Tcrd−/− mice is functionally compensated by other lymphocytes taking over genuine γδ T cell functions. To test this, we generated a knock-in model for diphtheria toxin–mediated conditional γδ T cell depletion. In contrast to IFN-γ–producing γδ T cells, IL-17–producing γδ T cells (Tγδ17 cells) recovered inefficiently after depletion, and their niches were filled by expanding Th17 cells and ILC3s. Complementary genetic fate mapping further demonstrated that Tγδ17 cells are long-lived and persisting lymphocytes. Investigating the function of γδ T cells, conditional depletion but not constitutive deficiency protected from imiquimod-induced psoriasis. Together, we clarify that fetal thymus-derived Tγδ17 cells are nonredundant local effector cells in IL-17–driven skin pathology.
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| 4. |
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| 5. |
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| 6. |
- Karah, Nabil, et al.
(author)
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The acinetobacter baumannii website (ab-web) : a multidisciplinary knowledge hub, communication platform, and workspace
- 2023
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In: FEMS Microbes. - : Oxford University Press. - 2633-6685. ; 4
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Research review (peer-reviewed)abstract
- Acinetobacter baumannii is a Gram-negative bacterium increasingly implicated in hospital-acquired infections and outbreaks. Effective prevention and control of such infections are commonly challenged by the frequent emergence of multidrug-resistant strains. Here we introduce Ab-web (https://www.acinetobacterbaumannii.no), the first online platform for sharing expertise on A. baumannii. Abweb is a species-centric knowledge hub, initially with 10 articles organized into two main sections, 'Overview' and 'Topics', and three themes, 'epidemiology', 'antibiotic resistance', and 'virulence'. The 'workspace' section provides a spot for colleagues to collaborate, build, and manage joint projects. Ab-web is a community-driven initiative amenable to constructive feedback and new ideas.
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| 7. |
- Wilharm, A, et al.
(author)
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Mutual interplay between IL-17-producing γδT cells and microbiota orchestrates oral mucosal homeostasis
- 2019
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In: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 1091-6490. ; 116:7, s. 2652-2661
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Journal article (peer-reviewed)abstract
- Elucidating the mechanisms by which homeostasis is established and maintained in the oral mucosa is important for human health. In this study we investigated the ontogeny and function of gingival γδT cells. The majority of these cells originate from embryonic precursors that maintain themselves locally independent of circulating γδT cells arising from adult precursors. The oral microbiota regulates the development of γδT cells, particularly those of embryonic origin. γδT cells represent the major sources of IL-17–producing cells in the gingiva, and they play a critical role in down-modulating gingival steady-state immunity that subsequently shapes oral microbial diversity. This suggests that oral homeostasis requires mutual interactions between γδT cells and the microbiota.
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