| 1. |
- Johnsson, Anna-Ida, et al.
(författare)
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Specific SCAR markers and multiplex real-time PCR for quantification of two Trichoderma biocontrol strains in environmental samples
- 2011
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Ingår i: BioControl. - : Springer Science and Business Media LLC. - 1386-6141 .- 1573-8248. ; 56, s. 903-913
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Tidskriftsartikel (refereegranskat)abstract
- Several strains from the genus Trichoderma (Ascomycetes, Hypocreales) are commercially used as biocontrol agents, e.g. in formulations containing the two Trichoderma strains IMI206039 (Hypocrea parapilulifera B.S. Lu, Druzhinina & Samuels) and IMI206040 (T. atroviride P. Karst). To quantify the presence of the two isolates after application, we developed primers for SCAR markers (Sequence-Characterised Amplified Region). In order to quantify both fungal strains simultaneously, we also designed fluorophore-labelled probes distinguishing the two strains, to be used in combination with the SCAR primers. In incubations of two different soils, artificially inoculated and maintained under controlled conditions, the quantification through amplification with the SCAR markers in qPCR and through colony-forming units from plate counting correlated well. Further tests of the markers on samples taken from a golf green treated with a product containing both strains indicated that the two biocontrol strains did not establish, either on the golf green or in the surrounding area.
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| 2. |
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| 3. |
- Von Rosen, Dietrich
(författare)
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Non-negative estimation of variance components in heteroscedastic one-way random-effects ANOVA models
- 2010
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Ingår i: Statistics. - : Informa UK Limited. - 0233-1888 .- 1029-4910. ; 44, s. 557-569
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Tidskriftsartikel (refereegranskat)abstract
- There is a considerable amount of literature dealing with inference about the parameters in a heteroscedastic one-way random-effects ANOVA model. In this paper, we primarily address the problem of improved quadratic estimation of the random-effect variance component. It turns out that such estimators with a smaller mean squared error compared with some standard unbiased quadratic estimators exist under quite general conditions. Improved estimators of the error variance components are also established.
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| 4. |
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| 5. |
- Skovsgaard, Jens Peter
(författare)
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Bog om tarmvridrøn
- 2013
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Ingår i: Skoven. - 0106-8539. ; 45, s. 551-551
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Book review N. Mayer & R.T. Klumpp (ed.) 2013: Elsbeere in Österreich – Monographie. Verein zur Erhaltung, Pflege und Vermarktung der Elsbeere - Genussregion Wiesenwienerwald Elsbeere, Michelbach.
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| 6. |
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| 7. |
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| 8. |
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| 9. |
- Messing, Ingmar
(författare)
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Land and land use : Natural preconditions
- 2011
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Ingår i: Agriculture and Forestry in Sweden since 1900- a cartographic description. - 9789187760617 ; , s. 14-22
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 10. |
- Messing, Ingmar
(författare)
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Marken i Sverige : Naturliga förutsättningar
- 2011
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Ingår i: Sveriges nationalatlas. Jordbruk och skogsbruk i Sverige sedan år 1900: en kartografisk beskrivning. - 9789187760594 ; , s. 14-22
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 11. |
- Tunon, Håkan, et al.
(författare)
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Kulturarv och hållbar utveckling
- 2010
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Ingår i: Bebyggelsehistorisk tidskrift. - : Föreningen Bebyggelsehistorisk tidskrift. - 0349-2834 .- 2002-3812. ; , s. 99–106-
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 12. |
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| 13. |
- Norman, Johan
(författare)
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Skogen – en plats för upplevelser
- 2011
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Ingår i: Grön Entreprenör, Naturupplevelse och hälsa – forskningen visar vägen. - 9789157690364 ; , s. 62-75
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Bokkapitel (populärvet., debatt m.m.)
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| 14. |
- Palsdottir, Anna Maria, et al.
(författare)
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Nature-Based Therapeutic Interventions
- 2011
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Ingår i: Forests, Trees and Human Health. - Dordrecht : Springer Netherlands. - 9789048198054 ; , s. 309-342
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 15. |
- Salomon, Björn, et al.
(författare)
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Evolutionary process of Hordeum brachyantherum 6x and related tetraploid species revealed by nuclear DNA sequences
- 2009
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Ingår i: Breeding Science. - : Japanese Society of Breeding. - 1344-7610 .- 1347-3735. ; 59, s. 611-616
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Tidskriftsartikel (refereegranskat)abstract
- A hexaploid form of Hordeum brachlyantherum ssp. brachyantherum was discovered in California in 1980, and its origin has since been studied over the past three decades. We applied EF-G, a nuclear DNA sequence, to infer the parents of the hexaploid form. In polyploid taxa, amplified DNAs were cloned into a vector, and EF-G copies were amplified from the colonies by PCR and digested with restriction enzymes to separate different types. Phylogenetic analysis was performed based on the DNA sequences. The result showed that H. brachyantherum ssp. brachyantherum 6x and 4x carried one identical DNA sequence of 910 bp, and had closely related DNA sequences of 931 bp. H. brachyantherum ssp. brachyantherum 6x and H. marinum ssp. gussoneanum 2x shared one identical DNA sequence of 915 bp. From these results we hypothesized that H. brachyantherum ssp. brachyantherum 6x has evolved by an outcrossing between H. marinum ssp. gussoneanum 2x and H. brachyantherum ssp. brachyantherum 4x, followed by a chromosome doubling. Our results also indicate that H. marinum was involved in the polyploidization of H. secalinum, H. capense, and H. marinum. The origins of H. jubatum and H. depressum are discussed.
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| 16. |
- Thulin, Carl-Gustaf
(författare)
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Utsättning av arter : möjlighet för bevarandebiologin
- 2010
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Ingår i: Biodiverse. - 1401-5064. ; 15, s. 8-9
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Tidskriftsartikel (populärvet., debatt m.m.)abstract
- Ett halvöppet, lövträdsdominerat landskap med betande hjortdjur, visenter, förvildad nötboskap och vildsvin, jagade av livskraftiga populationer av varg, björn och lo. Utopier och vilda fantasier? Kanske i delar, men något håller definitivt på att hända med bevarandet, återskapandet och skötseln av de europeiska vildmarkerna
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| 17. |
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Unraveling the logics of landscape
- 2014
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Samlingsverk (redaktörskap) (övrigt vetenskapligt/konstnärligt)abstract
- Despite conceptual oscillations through times, the concept of landscape remains highly subjective, whereupon unraveling its 'logics' opens up to a plurality of interpretations. Accordingly, by focusing on the interconnections present in the non-haphazard production of landscape, this publication elaborates on how the rural landscape is valued, monitored, changed, harbored, used and misused, be it through actions, representations or metaphors. This book covers a broad range of topics, with contributions from scholars from more than 30 countries.
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| 18. |
- Messing, Ingmar, et al.
(författare)
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Methods to estimate lettuce evapotranspiration in greenhouse conditions in the central zone of Chile
- 2009
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Ingår i: Chilean Journal of Agricultural Research. - 0718-5820. ; 69, s. 60-70
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Tidskriftsartikel (refereegranskat)abstract
- This study evaluates five methods to estimate crop evapotranspiration in greenhouse conditions. It compares their performance in relation to the evapotranspiration directly determined from water balance measurements (ET(lys)) in an irrigated lettuce (Lactuca sativa L.) crop during 9 weeks. Daily values of the reference evapotranspiration (ETo) from Class A pan (CAP), Piche atmometers (ATM), Andersson evaporimeters (ANE), FAO-Radiation (FRE) and FAO-Penman-Monteith (PME) equations were compared. The methods showed similar temporal variations but at different ranges as follows: ANE < CAP < FRE < PME < ATM. Furthemore, ETo had a clear correlation with solar radiation. Crop coefficients (Kc = ET(lys)/ETo) varied somewhat amongst the methods, but trends were identified for two periods: in the first week, the overall mean Kc was 0.3 (+/- 0.1) and in weeks 2 to 9 on average 0.6 (+/- 0.3). The greenhouse values of Kc were lower than those generally adopted for lettuce in field conditions. In terms of irrigation design, crop evapotranspiration can be estimated by the methods in this study, on the condition that the appropriate crop coefficients are applied. The fact that ANE showed values closest to those of ETlys, along with cost and management convenience, makes it an advantageous alternative as compared to the other methods.
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| 19. |
- Crous, P. W., et al.
(författare)
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Fusarium : more than a node or a foot-shaped basal cell
- 2021
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Ingår i: Studies in mycology. - : CENTRAALBUREAU SCHIMMELCULTURE. - 0166-0616 .- 1872-9797. ; :98
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Tidskriftsartikel (refereegranskat)abstract
- Recent publications have argued that there are potentially serious consequences for researchers in recognising distinct genera in the terminal fusarioid clade of the family Nectriaceae. Thus, an alternate hypothesis, namely a very broad concept of the genus Fusarium was proposed. In doing so, however, a significant body of data that supports distinct genera in Nectriaceae based on morphology, biology, and phylogeny is disregarded. A DNA phylogeny based on 19 orthologous protein-coding genes was presented to support a very broad concept of Fusarium at the F1 node in Nectriaceae. Here, we demonstrate that re-analyses of this dataset show that all 19 genes support the F3 node that represents Fusarium sensu stricto as defined by F. sambucinum (sexual morph synonym Gibberella pulicaris). The backbone of the phylogeny is resolved by the concatenated alignment, but only six of the 19 genes fully support the F1 node, representing the broad circumscription of Fusarium. Furthermore, a re-analysis of the concatenated dataset revealed alternate topologies in different phylogenetic algorithms, highlighting the deep divergence and unresolved placement of various Nectriaceae lineages proposed as members of Fusarium. Species of Fusarium s. str. are characterised by Gibberella sexual morphs, asexual morphs with thin- or thick-walled macroconidia that have variously shaped apical and basal cells, and trichothecene mycotoxin production, which separates them from other fusarioid genera. Here we show that the Wollenweber concept of Fusarium presently accounts for 20 segregate genera with clear-cut synapomorphic traits, and that fusarioid macroconidia represent a character that has been gained or lost multiple times throughout Nectriaceae. Thus, the very broad circumscription of Fusarium is blurry and without apparent synapomorphies, and does not include all genera with fusarium-like macroconidia, which are spread throughout Nectriaceae (e.g., Cosmosporella, Macroconia, Microcera). In this study four new genera are introduced, along with 18 new species and 16 new combinations. These names convey information about relationships, morphology, and ecological preference that would otherwise be lost in a broader definition of Fusarium. To assist users to correctly identify fusarioid genera and species, we introduce a new online identification database, Fusarioid-ID, accessible at www.fusarium.org. The database comprises partial sequences from multiple genes commonly used to identify fusarioid taxa (act1, CaM, his3, rpb1, rpb2, tef1, tub2, ITS, and LSU). In this paper, we also present a nomenclator of names that have been introduced in Fusarium up to January 2021 as well as their current status, types, and diagnostic DNA barcode data. In this study, researchers from 46 countries, representing taxonomists, plant pathologists, medical mycologists, quarantine officials, regulatory agencies, and students, strongly support the application and use of a more precisely delimited Fusarium (= Gibberella) concept to accommodate taxa from the robust monophyletic node F3 on the basis of a well-defined and unique combination of morphological and biochemical features. This F3 node includes, among others, species of the F. fujikuroi, F. incarnatum-equiseti, F. oxysporum, and F. sambucinum species complexes, but not species of Bisifusarium [F. dimerum species complex (SC)], Cyanonectria (F. buxicola SC), Geejayessia (F. staphyleae SC), Neocosmospora (F. solani SC) or Rectifusarium (F. ventricosum SC). The present study represents the first step to generating a new online monograph of Fusarium and allied fusarioid genera (www.fusarium.org).
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| 20. |
- Mattsson, Eskil, 1981, et al.
(författare)
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Quantification of carbon stock and tree diversity of homegardens in a dry zone area of Moneragala District, Sri Lanka
- 2015
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Ingår i: Agroforestry Systems. - : Springer Science and Business Media LLC. - 1572-9680 .- 0167-4366. ; 89:3, s. 435-445
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Tidskriftsartikel (refereegranskat)abstract
- Homegarden agroforestry systems are suggested to hold a large potential for climate change mitigation and adaptation. This is due to their multifunctional role in providing income, food and ecosystem services while decreasing pressure on natural forests and hence saving and storing carbon. In this paper, above-ground biomass carbon and tree species diversity of trees was quantified in homegardens around two villages in the dry south-eastern part of Moneragala district of Sri Lanka. A total of 45 dry zone homegardens were sampled on size, diameter at breast height, tree height and species diversity. Using allometric equations, we find a mean above-ground biomass stock of 13 mega grams of carbon per hectare (Mg C ha−1) with a large range among homegardens (1–56 Mg C ha−1, n = 45) due to a variation of tree diversity and composition between individual homegardens. Mean above-ground carbon stock per unit area was higher in small homegardens (0.2 ha, 26 Mg C ha−1, n = 11) and statistically different compared to medium (0.4–0.8 ha, 9 Mg C ha−1, n = 27) and large (1.0–1.2 ha, 8 Mg C ha−1, n = 7) homegardens. In total, 4,278 trees were sampled and 70 tree species identified and recorded. The Shannon Wiener index were used to evaluate diversity per homegarden and ranged from 0.76 to 3.01 with a mean value of 2.05 ± 0.07 indicating a medium evenly distributed diversity of sampled tree species. The results show a vast heterogeneity in terms of carbon stock and tree diversity within the less studied dry zone homegardens; results that contribute to more knowledge of their expansion potential as well as climate mitigation and adaptation potential. The results are also useful for whether homegardens should be considered to be included as an activity to enhance natural forest cover within Sri Lanka’s newly commenced UN-REDD National Programme.
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| 21. |
- Benavent-Celma, Clara, et al.
(författare)
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Current practices and emerging possibilities for reducing the spread of oomycete pathogens in terrestrial and aquatic production systems in the European Union
- 2022
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Ingår i: Fungal Biology Reviews. - : Elsevier. - 1749-4613 .- 1878-0253. ; 40, s. 19-36
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Tidskriftsartikel (refereegranskat)abstract
- Diseases caused by oomycete pathogens are a global threat to forestry, agriculture and aquaculture. Because of their complex life cycles, characterised by dormant resting structures that enable their survival for years under hostile environmental conditions, reducing the spread of oomycetes is a challenging task. In this review, we present an overview of this challenge, starting from the need to understand the natural and anthropogenic dispersal pathways of these pathogens. Focusing on the European Union, we explore current legislation that forms a backbone for biosecurity protocols against the spread of oomycetes through trade and transport. We discuss the options for prevention, containment and long-term management of oomycetes in different production settings, emphasising the importance of prevention as the most cost-efficient strategy to reduce the spread of these pathogens. Finally, we highlight some of the new and emerging technologies and strategies as potential tools in the integrated pest management of animal and plant diseases caused by oomycetes. We emphasise the urgency of actions to halt the global spread of these pathogens.
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| 22. |
- Ritter, Camila, et al.
(författare)
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Biodiversity assessments in the 21st century: The potential of insect traps to complement environmental samples for estimating eukaryotic and prokaryotic diversity using high-throughput DNA metabarcoding.
- 2019
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Ingår i: Genome. - : Canadian Science Publishing. - 1480-3321 .- 0831-2796. ; 62:3, s. 147-159
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Tidskriftsartikel (refereegranskat)abstract
- The rapid loss of biodiversity, coupled with difficulties in species identification, call for innovative approaches to assess biodiversity. Insects make up a substantial proportion of extant diversity and play fundamental roles in any given ecosystem. To complement morphological species identification, new techniques such as metabarcoding make it possible to quantify insect diversity and insect-ecosystem interactions through DNA sequencing. Here we examine the potential of bulk insect samples (i.e., containing many non-sorted specimens) to assess prokaryote and eukaryote biodiversity and to complement the taxonomic coverage of soil samples. We sampled 25 sites on three continents and in various ecosystems, collecting insects with Slam-traps (Brazil) and Malaise-traps (South Africa and Sweden). We then compared our diversity estimates with the results obtained with biodiversity data from soil samples from the same localities. We found a largely different taxonomic composition between the soil and insect samples, testifying to the potential of bulk insect samples to complement soil samples. Finally, we found that non-destructive DNA extraction protocols, which preserve insect specimens for morphological studies, constitute a promising choice for cost-effective biodiversity assessments. We propose that the sampling and sequencing of insect samples should become a standard complement for biodiversity studies based on environmental DNA.
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| 23. |
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| 24. |
- Messing, Ingmar, et al.
(författare)
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Rovdrift på åkermark i Uppsala
- 2012
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Ingår i: Upsala nya tidning. - 1104-0173. ; , s. 5-
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Annan publikation (populärvet., debatt m.m.)
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| 25. |
- Van Lun, Michiel, et al.
(författare)
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Subunit Interface Dynamics in Hexadecameric Rubisco
- 2011
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Ingår i: Journal of Molecular Biology. - : Elsevier BV. - 0022-2836 .- 1089-8638. ; 411:5, s. 1083-1098
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Tidskriftsartikel (refereegranskat)abstract
- Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (Rubisco) plays an important role in the global carbon cycle as a hub for biomass. Rubisco catalyzes not only the carboxylation of RuBP with carbon dioxide but also a competing oxygenation reaction of RuBP with a negative impact on photosynthetic yield. The functional active site is built from two large (L) subunits that form a dimer. The octameric core of four L(2) dimers is held at each end by a cluster of four small (S) subunits, forming a hexadecamer. Each large subunit contacts more than one S subunit. These interactions exploit the dynamic flexibility of Rubisco, which we address in this study. Here, we describe seven different types of interfaces of hexadecameric Rubisco. We have analyzed these interfaces with respect to the size of the interface area and the number of polar interactions, including salt bridges and hydrogen bonds in a variety of Rubisco enzymes from different organisms and different kingdoms of life, including the Rubisco-like proteins. We have also performed molecular dynamics simulations of Rubisco from Chlamydomonas reinhardtii and mutants thereof. From our computational analyses, we propose structural checkpoints of the S subunit to ensure the functionality and/or assembly of the Rubisco holoenzyme. These checkpoints appear to fine-tune the dynamics of the enzyme in a way that could influence enzyme performance.
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