| 1. |
- Johnsson, Anna-Ida, et al.
(author)
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Specific SCAR markers and multiplex real-time PCR for quantification of two Trichoderma biocontrol strains in environmental samples
- 2011
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In: BioControl. - : Springer Science and Business Media LLC. - 1386-6141 .- 1573-8248. ; 56, s. 903-913
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Journal article (peer-reviewed)abstract
- Several strains from the genus Trichoderma (Ascomycetes, Hypocreales) are commercially used as biocontrol agents, e.g. in formulations containing the two Trichoderma strains IMI206039 (Hypocrea parapilulifera B.S. Lu, Druzhinina & Samuels) and IMI206040 (T. atroviride P. Karst). To quantify the presence of the two isolates after application, we developed primers for SCAR markers (Sequence-Characterised Amplified Region). In order to quantify both fungal strains simultaneously, we also designed fluorophore-labelled probes distinguishing the two strains, to be used in combination with the SCAR primers. In incubations of two different soils, artificially inoculated and maintained under controlled conditions, the quantification through amplification with the SCAR markers in qPCR and through colony-forming units from plate counting correlated well. Further tests of the markers on samples taken from a golf green treated with a product containing both strains indicated that the two biocontrol strains did not establish, either on the golf green or in the surrounding area.
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| 2. |
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| 3. |
- Von Rosen, Dietrich
(author)
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Non-negative estimation of variance components in heteroscedastic one-way random-effects ANOVA models
- 2010
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In: Statistics. - : Informa UK Limited. - 0233-1888 .- 1029-4910. ; 44, s. 557-569
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Journal article (peer-reviewed)abstract
- There is a considerable amount of literature dealing with inference about the parameters in a heteroscedastic one-way random-effects ANOVA model. In this paper, we primarily address the problem of improved quadratic estimation of the random-effect variance component. It turns out that such estimators with a smaller mean squared error compared with some standard unbiased quadratic estimators exist under quite general conditions. Improved estimators of the error variance components are also established.
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| 4. |
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| 5. |
- Skovsgaard, Jens Peter
(author)
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Bog om tarmvridrøn
- 2013
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In: Skoven. - 0106-8539. ; 45, s. 551-551
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Other publication (other academic/artistic)abstract
- Book review N. Mayer & R.T. Klumpp (ed.) 2013: Elsbeere in Österreich – Monographie. Verein zur Erhaltung, Pflege und Vermarktung der Elsbeere - Genussregion Wiesenwienerwald Elsbeere, Michelbach.
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| 6. |
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| 7. |
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| 8. |
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| 9. |
- Norman, Johan
(author)
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Skogen – en plats för upplevelser
- 2011
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In: Grön Entreprenör, Naturupplevelse och hälsa – forskningen visar vägen. - 9789157690364 ; , s. 62-75
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Book chapter (pop. science, debate, etc.)
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| 10. |
- Palsdottir, Anna Maria, et al.
(author)
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Nature-Based Therapeutic Interventions
- 2011
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In: Forests, Trees and Human Health. - Dordrecht : Springer Netherlands. - 9789048198054 ; , s. 309-342
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Book chapter (other academic/artistic)
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| 11. |
- Salomon, Björn, et al.
(author)
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Evolutionary process of Hordeum brachyantherum 6x and related tetraploid species revealed by nuclear DNA sequences
- 2009
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In: Breeding Science. - : Japanese Society of Breeding. - 1344-7610 .- 1347-3735. ; 59, s. 611-616
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Journal article (peer-reviewed)abstract
- A hexaploid form of Hordeum brachlyantherum ssp. brachyantherum was discovered in California in 1980, and its origin has since been studied over the past three decades. We applied EF-G, a nuclear DNA sequence, to infer the parents of the hexaploid form. In polyploid taxa, amplified DNAs were cloned into a vector, and EF-G copies were amplified from the colonies by PCR and digested with restriction enzymes to separate different types. Phylogenetic analysis was performed based on the DNA sequences. The result showed that H. brachyantherum ssp. brachyantherum 6x and 4x carried one identical DNA sequence of 910 bp, and had closely related DNA sequences of 931 bp. H. brachyantherum ssp. brachyantherum 6x and H. marinum ssp. gussoneanum 2x shared one identical DNA sequence of 915 bp. From these results we hypothesized that H. brachyantherum ssp. brachyantherum 6x has evolved by an outcrossing between H. marinum ssp. gussoneanum 2x and H. brachyantherum ssp. brachyantherum 4x, followed by a chromosome doubling. Our results also indicate that H. marinum was involved in the polyploidization of H. secalinum, H. capense, and H. marinum. The origins of H. jubatum and H. depressum are discussed.
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| 12. |
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Unraveling the logics of landscape
- 2014
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Editorial collection (other academic/artistic)abstract
- Despite conceptual oscillations through times, the concept of landscape remains highly subjective, whereupon unraveling its 'logics' opens up to a plurality of interpretations. Accordingly, by focusing on the interconnections present in the non-haphazard production of landscape, this publication elaborates on how the rural landscape is valued, monitored, changed, harbored, used and misused, be it through actions, representations or metaphors. This book covers a broad range of topics, with contributions from scholars from more than 30 countries.
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| 13. |
- Messing, Ingmar, et al.
(author)
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Methods to estimate lettuce evapotranspiration in greenhouse conditions in the central zone of Chile
- 2009
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In: Chilean Journal of Agricultural Research. - 0718-5820. ; 69, s. 60-70
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Journal article (peer-reviewed)abstract
- This study evaluates five methods to estimate crop evapotranspiration in greenhouse conditions. It compares their performance in relation to the evapotranspiration directly determined from water balance measurements (ET(lys)) in an irrigated lettuce (Lactuca sativa L.) crop during 9 weeks. Daily values of the reference evapotranspiration (ETo) from Class A pan (CAP), Piche atmometers (ATM), Andersson evaporimeters (ANE), FAO-Radiation (FRE) and FAO-Penman-Monteith (PME) equations were compared. The methods showed similar temporal variations but at different ranges as follows: ANE < CAP < FRE < PME < ATM. Furthemore, ETo had a clear correlation with solar radiation. Crop coefficients (Kc = ET(lys)/ETo) varied somewhat amongst the methods, but trends were identified for two periods: in the first week, the overall mean Kc was 0.3 (+/- 0.1) and in weeks 2 to 9 on average 0.6 (+/- 0.3). The greenhouse values of Kc were lower than those generally adopted for lettuce in field conditions. In terms of irrigation design, crop evapotranspiration can be estimated by the methods in this study, on the condition that the appropriate crop coefficients are applied. The fact that ANE showed values closest to those of ETlys, along with cost and management convenience, makes it an advantageous alternative as compared to the other methods.
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| 14. |
- Crous, P. W., et al.
(author)
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Fusarium : more than a node or a foot-shaped basal cell
- 2021
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In: Studies in mycology. - : CENTRAALBUREAU SCHIMMELCULTURE. - 0166-0616 .- 1872-9797. ; :98
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Journal article (peer-reviewed)abstract
- Recent publications have argued that there are potentially serious consequences for researchers in recognising distinct genera in the terminal fusarioid clade of the family Nectriaceae. Thus, an alternate hypothesis, namely a very broad concept of the genus Fusarium was proposed. In doing so, however, a significant body of data that supports distinct genera in Nectriaceae based on morphology, biology, and phylogeny is disregarded. A DNA phylogeny based on 19 orthologous protein-coding genes was presented to support a very broad concept of Fusarium at the F1 node in Nectriaceae. Here, we demonstrate that re-analyses of this dataset show that all 19 genes support the F3 node that represents Fusarium sensu stricto as defined by F. sambucinum (sexual morph synonym Gibberella pulicaris). The backbone of the phylogeny is resolved by the concatenated alignment, but only six of the 19 genes fully support the F1 node, representing the broad circumscription of Fusarium. Furthermore, a re-analysis of the concatenated dataset revealed alternate topologies in different phylogenetic algorithms, highlighting the deep divergence and unresolved placement of various Nectriaceae lineages proposed as members of Fusarium. Species of Fusarium s. str. are characterised by Gibberella sexual morphs, asexual morphs with thin- or thick-walled macroconidia that have variously shaped apical and basal cells, and trichothecene mycotoxin production, which separates them from other fusarioid genera. Here we show that the Wollenweber concept of Fusarium presently accounts for 20 segregate genera with clear-cut synapomorphic traits, and that fusarioid macroconidia represent a character that has been gained or lost multiple times throughout Nectriaceae. Thus, the very broad circumscription of Fusarium is blurry and without apparent synapomorphies, and does not include all genera with fusarium-like macroconidia, which are spread throughout Nectriaceae (e.g., Cosmosporella, Macroconia, Microcera). In this study four new genera are introduced, along with 18 new species and 16 new combinations. These names convey information about relationships, morphology, and ecological preference that would otherwise be lost in a broader definition of Fusarium. To assist users to correctly identify fusarioid genera and species, we introduce a new online identification database, Fusarioid-ID, accessible at www.fusarium.org. The database comprises partial sequences from multiple genes commonly used to identify fusarioid taxa (act1, CaM, his3, rpb1, rpb2, tef1, tub2, ITS, and LSU). In this paper, we also present a nomenclator of names that have been introduced in Fusarium up to January 2021 as well as their current status, types, and diagnostic DNA barcode data. In this study, researchers from 46 countries, representing taxonomists, plant pathologists, medical mycologists, quarantine officials, regulatory agencies, and students, strongly support the application and use of a more precisely delimited Fusarium (= Gibberella) concept to accommodate taxa from the robust monophyletic node F3 on the basis of a well-defined and unique combination of morphological and biochemical features. This F3 node includes, among others, species of the F. fujikuroi, F. incarnatum-equiseti, F. oxysporum, and F. sambucinum species complexes, but not species of Bisifusarium [F. dimerum species complex (SC)], Cyanonectria (F. buxicola SC), Geejayessia (F. staphyleae SC), Neocosmospora (F. solani SC) or Rectifusarium (F. ventricosum SC). The present study represents the first step to generating a new online monograph of Fusarium and allied fusarioid genera (www.fusarium.org).
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| 15. |
- Mattsson, Eskil, 1981, et al.
(author)
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Quantification of carbon stock and tree diversity of homegardens in a dry zone area of Moneragala District, Sri Lanka
- 2015
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In: Agroforestry Systems. - : Springer Science and Business Media LLC. - 1572-9680 .- 0167-4366. ; 89:3, s. 435-445
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Journal article (peer-reviewed)abstract
- Homegarden agroforestry systems are suggested to hold a large potential for climate change mitigation and adaptation. This is due to their multifunctional role in providing income, food and ecosystem services while decreasing pressure on natural forests and hence saving and storing carbon. In this paper, above-ground biomass carbon and tree species diversity of trees was quantified in homegardens around two villages in the dry south-eastern part of Moneragala district of Sri Lanka. A total of 45 dry zone homegardens were sampled on size, diameter at breast height, tree height and species diversity. Using allometric equations, we find a mean above-ground biomass stock of 13 mega grams of carbon per hectare (Mg C ha−1) with a large range among homegardens (1–56 Mg C ha−1, n = 45) due to a variation of tree diversity and composition between individual homegardens. Mean above-ground carbon stock per unit area was higher in small homegardens (0.2 ha, 26 Mg C ha−1, n = 11) and statistically different compared to medium (0.4–0.8 ha, 9 Mg C ha−1, n = 27) and large (1.0–1.2 ha, 8 Mg C ha−1, n = 7) homegardens. In total, 4,278 trees were sampled and 70 tree species identified and recorded. The Shannon Wiener index were used to evaluate diversity per homegarden and ranged from 0.76 to 3.01 with a mean value of 2.05 ± 0.07 indicating a medium evenly distributed diversity of sampled tree species. The results show a vast heterogeneity in terms of carbon stock and tree diversity within the less studied dry zone homegardens; results that contribute to more knowledge of their expansion potential as well as climate mitigation and adaptation potential. The results are also useful for whether homegardens should be considered to be included as an activity to enhance natural forest cover within Sri Lanka’s newly commenced UN-REDD National Programme.
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| 16. |
- Van Lun, Michiel, et al.
(author)
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Subunit Interface Dynamics in Hexadecameric Rubisco
- 2011
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In: Journal of Molecular Biology. - : Elsevier BV. - 0022-2836 .- 1089-8638. ; 411:5, s. 1083-1098
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Journal article (peer-reviewed)abstract
- Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (Rubisco) plays an important role in the global carbon cycle as a hub for biomass. Rubisco catalyzes not only the carboxylation of RuBP with carbon dioxide but also a competing oxygenation reaction of RuBP with a negative impact on photosynthetic yield. The functional active site is built from two large (L) subunits that form a dimer. The octameric core of four L(2) dimers is held at each end by a cluster of four small (S) subunits, forming a hexadecamer. Each large subunit contacts more than one S subunit. These interactions exploit the dynamic flexibility of Rubisco, which we address in this study. Here, we describe seven different types of interfaces of hexadecameric Rubisco. We have analyzed these interfaces with respect to the size of the interface area and the number of polar interactions, including salt bridges and hydrogen bonds in a variety of Rubisco enzymes from different organisms and different kingdoms of life, including the Rubisco-like proteins. We have also performed molecular dynamics simulations of Rubisco from Chlamydomonas reinhardtii and mutants thereof. From our computational analyses, we propose structural checkpoints of the S subunit to ensure the functionality and/or assembly of the Rubisco holoenzyme. These checkpoints appear to fine-tune the dynamics of the enzyme in a way that could influence enzyme performance.
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| 17. |
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| 18. |
- Finlay, Roger
(author)
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Role of Mycorrhizal Symbioses in Phosphorus Cycling
- 2011
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In: Phosphorus in Action - Biological Processes in Soil Phosphorus Cycling. - Berlin, Heidelberg : Springer Berlin Heidelberg. - 9783642152702 ; 26:26, s. 137-168
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Book chapter (peer-reviewed)
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| 19. |
- Inselsbacher, Erich, et al.
(author)
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The potential of microdialysis to monitor organic and inorganic nitrogen compounds in soil
- 2011
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In: Soil Biology and Biochemistry. - : Elsevier BV. - 0038-0717 .- 1879-3428. ; 43, s. 1321-1332
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Journal article (peer-reviewed)abstract
- Plant nitrogen (N) acquisition is strongly controlled by the concentration of available inorganic and organic N in the soil solution and by biogeochemical processes in the rhizosphere. However, until now it was hardly possible to reliably estimate plant-available N in soil microsites. Here, a novel microdialysis approach based on passive diffusion sampling is presented and compared qualitatively and quantitatively with lysimeter and soil extraction techniques when analyzing two contrasting boreal soils. Further, preliminary dialysis membrane calibration issues for sampling plant-available N compounds are discussed. Due to its miniaturized design microdialysis was shown to be a suitable tool for continuous sampling of ammonium, nitrate and free amino acids from the soil solution with only minimal disturbance of the soil structure. Microdialysis proved to be outstanding regarding the possible spatial (<0.5 mm) and temporal (<30 min) resolution of soil solution N chemistry. The different methods for soil N sampling resulted in significantly different results. In lysimeter and soil extraction samples, nitrate and ammonium were found at the highest concentrations, while results from microdialysis revealed that the pool of plant-available amino acids was contributing most to the total N pool tested. Application of a standard N solution to the tested soils led to an immediate peak of recovery via the microdialysis probes followed by a rapid decrease due to the formation of a depletion zone at the probe surfaces. Therefore, this relatively new technique will not only provide essential data on diffusion rates of a variety of N compounds in the soil but might be used for monitoring quantitative and qualitative changes in plant-available N in soil microsites such as the rhizosphere. (C) 2011 Elsevier Ltd. All rights reserved.
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| 20. |
- Nilsson, Marie-Louise, et al.
(author)
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Current-use and Organochlorine Pesticides and Polychlorinated Biphenyls in the Biodegradable Fraction of Source Separated Household Waste, Compost, and Anaerobic Digestate
- 2011
-
In: Bulletin of Environmental Contamination and Toxicology. - : Springer Science and Business Media LLC. - 0007-4861 .- 1432-0800. ; 86, s. 60-64
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Journal article (peer-reviewed)abstract
- Several current-use (a parts per thousand currency sign80 ng g(-1) dry weight) and organochlorine pesticides (a parts per thousand currency sign15 ng g(-1) dry weight) and polychlorinated biphenyls (a parts per thousand currency sign18 ng g(-1) dry weight) were found in the biodegradable fraction of source separated household waste, compost, and/or anaerobic digestate. The degradation rates of individual compounds differ depending on the treatment. Dieldrin and pentachloroaniline, e.g., degrade more rapidly than the waste is mineralized and accumulates in the products after all treatments. Many organochlorines degrade at the same rate as the waste and have the same concentrations in the waste and products. Chlorpyrifos degrades slower than the waste and accumulates in all products and ethion during anaerobic digestion. The polychlorinated biphenyls and some pesticides show different degradations rates relative the waste during different processes. Understanding the degradation of the contaminants under different conditions is necessary to develop quality criteria for the use of compost and digestate.
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| 21. |
- Salomon, Björn
(author)
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The origin of the H, St, W, and Y genomes in allotetraploid species of Elymus L. and Stenostachys Turcz. (Poaceae: Triticeae)
- 2011
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In: Plant Systematics and Evolution. - : Springer Science and Business Media LLC. - 0378-2697 .- 1615-6110. ; 291, s. 197-210
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Journal article (peer-reviewed)abstract
- Based on sequences from two single-copy nuclear genes (DMC1 and EF-G), four plastid genes (rbcL, rpoA, matK, and ndhF), and one mitochondrial gene (coxII), we investigate the origin of the H, St, W, and Y genomes in four allotetraploid species of Elymus and two allotetraploid species of Stenostachys. Despite significant incongruence between the two nuclear genes and between the nuclear and organelle data partitions, individual and combined analyses of the data partitions unequivocally show that the St and H genomes of the tetraploid American species of Elymus are derived from Pseudoroegneria and Hordeum, respectively, with Pseudoroegneria serving as the female parent, and that the H and W genomes of Stenostachys are derived from Hordeum and Australopyrum, respectively, with Hordeum serving as the female parent. The analyses equally clearly demonstrate that the St genome of the tetraploid Asiatic Elymus species is derived from Pseudoroegneria, with the latter serving as the female parent, but the relationship of the Y genome is less clear. Individual analyses of the nuclear genes provide conflicting results, but combined analysis of all data suggests a sister group relationship to Heteranthelium, albeit without any jackknife support.
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| 22. |
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| 23. |
- Tasin, Marco, et al.
(author)
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Chemical Ecology and Management of Lobesia botrana (Lepidoptera: Tortricidae)
- 2011
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In: Journal of Economic Entomology. - 0022-0493 .- 1938-291X. ; 104, s. 1125-1137
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Journal article (peer-reviewed)abstract
- The moth Lobesia botrana (Denis & Schiffermuller) (Lepidoptera: Tortricidae) feeds on grapes (Vitis vinifera L.), reducing yield and increasing susceptibility to fungal infections. L. botrana is among the most economically important insects in Europe and has recently been found in vineyards in Chile, Argentina, and California. Here, we review L. botrana biology and behavior in relation to its larval host (the grapevine) and its natural enemies. We also discuss current and future control strategies in light of our knowledge of chemical ecology, with an emphasis on the use of the sex pheromone-based strategies as an environmentally safe management approach. Pheromone-mediated mating disruption is the most promising technique available on grapes and is currently implemented on approximate to 140,000 ha in Europe. Experience from several growing areas confirms the importance of collaboration between research, extension, growers, and pheromone-supply companies for the successful implementation of the mating disruption technique. In the vineyards where mating disruption has been successfully applied as an areawide strategy, the reduction in insecticide use has improved the quality of life for growers, consumers, as well as the public living near wine-growing areas and has thereby reduced the conflict between agricultural and urban communities.
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| 24. |
- Tenngart, Carina, et al.
(author)
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Patients' experiences and use of a therapeutic garden: from a designer's perspective
- 2010
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In: Schweizerische Zeitschrift Für Forstwesen = Swiss Forestry Journal. - : Swiss Forestry Society. - 0036-7818. ; 161, s. 104-113
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Journal article (peer-reviewed)abstract
- The object of this study is to broaden our knowledge on how a therapeutic landscape is used and experienced by its patients. Data refer to qualitative, semi-structured and hour-long interviews with ten patients in a Swedish healing garden. All patients have stress-related diseases and are participants in a rehabilitation programme with nature-assisted therapy. A thematic analysis resulted in two main themes: “to escape, observe and get sensory stimulation” and “to achieve satisfaction, socialize and re-evaluate”, with two and three sub-themes respectively. To grasp both the experience and the use, the themes are described both as phenomena and in relation to where they occur. The experiences described and how they might be aspects of healing, as well as the need to include a perspective related to this process in the design of therapeutic settings, is elaborated on in the discussion. Aspects of results in the thematic analysis are discussed with relation to design and theories in environmental psychology and can be seen as a kind of evaluation of the design of the garden. Also discussed is how this information can be used to contribute to a more evaluation-based knowledge in the design of therapeutic settings.
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| 25. |
- Witzgall, Peter
(author)
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Sex pheromones and their impact on pest management
- 2010
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In: Journal of Chemical Ecology. - : Springer Science and Business Media LLC. - 0098-0331 .- 1573-1561. ; 36, s. 80-100
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Journal article (peer-reviewed)abstract
- The idea of using species-specific behavior-modifying chemicals for the management of noxious insects in agriculture, horticulture, forestry, stored products, and for insect vectors of diseases has been a driving ambition through five decades of pheromone research. Hundreds of pheromones and other semiochemicals have been discovered that are used to monitor the presence and abundance of insects and to protect plants and animals against insects. The estimated annual production of lures for monitoring and mass trapping is on the order of tens of millions, covering at least 10 million hectares. Insect populations are controlled by air permeation and attract-and-kill techniques on at least 1 million hectares. Here, we review the most important and widespread practical applications. Pheromones are increasingly efficient at low population densities, they do not adversely affect natural enemies, and they can, therefore, bring about a long-term reduction in insect populations that cannot be accomplished with conventional insecticides. A changing climate with higher growing season temperatures and altered rainfall patterns makes control of native and invasive insects an increasingly urgent challenge. Intensified insecticide use will not provide a solution, but pheromones and other semiochemicals instead can be implemented for sustainable area-wide management and will thus improve food security for a growing population. Given the scale of the challenges we face to mitigate the impacts of climate change, the time is right to intensify goal-oriented interdisciplinary research on semiochemicals, involving chemists, entomologists, and plant protection experts, in order to provide the urgently needed, and cost-effective technical solutions for sustainable insect management worldwide.
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