| 1. |
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| 2. |
- Liljenström, Hans, et al.
(författare)
-
Scoping Report on Socio-Economic and Land Use Dynamics in the Stockholm-Mälar Region
- 2014
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- This scoping report for the COMPLEX work package 4 (WP4) on Socio-Economic and Land Use Dynamics in the Stockholm-Mälar Region has several objectives. The first is to present and outline the state-of-the-art, including a literature review, with regard to complex pathways to a low carbon society, with special relevance to the Stockholm-Mälar region. It will do so in a larger context of complex socio-natural systems, with an emphasis on a green economy, bioen-ergy and land use, as well as on societal transformation and behavioural change. Another objec-tive is to inform primarily our partners in COMPLEX, but also others who might be interested, including stakeholders in our study region. Perhaps the most important objective with this re-port is to provide a basis for the collaborative work within our work package, and with our col-leagues elsewhere. In this second revised version of the report, we have extended some parts, and shortened others, depending on suggestions and interesting new information. A part of this new information has come from a stakeholder workshop held in Sigtuna in early 2014, which to a great extent could contribute to a more elaborate analysis of stakeholder and policy maker positions. In addition, there is now a more extensive description of relevant land use modelling. The illustrations included in this report are extracted from scientific articles and other material that we are referring to. Their origin is explicitly cited but no copyright authorization has been requested, while this report is intended primarily for internal spread and use within the COM-PLEX consortium and its networks.
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| 3. |
- Johnsson, Anna-Ida, et al.
(författare)
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Specific SCAR markers and multiplex real-time PCR for quantification of two Trichoderma biocontrol strains in environmental samples
- 2011
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Ingår i: BioControl. - : Springer Science and Business Media LLC. - 1386-6141 .- 1573-8248. ; 56, s. 903-913
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Tidskriftsartikel (refereegranskat)abstract
- Several strains from the genus Trichoderma (Ascomycetes, Hypocreales) are commercially used as biocontrol agents, e.g. in formulations containing the two Trichoderma strains IMI206039 (Hypocrea parapilulifera B.S. Lu, Druzhinina & Samuels) and IMI206040 (T. atroviride P. Karst). To quantify the presence of the two isolates after application, we developed primers for SCAR markers (Sequence-Characterised Amplified Region). In order to quantify both fungal strains simultaneously, we also designed fluorophore-labelled probes distinguishing the two strains, to be used in combination with the SCAR primers. In incubations of two different soils, artificially inoculated and maintained under controlled conditions, the quantification through amplification with the SCAR markers in qPCR and through colony-forming units from plate counting correlated well. Further tests of the markers on samples taken from a golf green treated with a product containing both strains indicated that the two biocontrol strains did not establish, either on the golf green or in the surrounding area.
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| 4. |
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| 5. |
- Von Rosen, Dietrich
(författare)
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Non-negative estimation of variance components in heteroscedastic one-way random-effects ANOVA models
- 2010
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Ingår i: Statistics. - : Informa UK Limited. - 0233-1888 .- 1029-4910. ; 44, s. 557-569
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Tidskriftsartikel (refereegranskat)abstract
- There is a considerable amount of literature dealing with inference about the parameters in a heteroscedastic one-way random-effects ANOVA model. In this paper, we primarily address the problem of improved quadratic estimation of the random-effect variance component. It turns out that such estimators with a smaller mean squared error compared with some standard unbiased quadratic estimators exist under quite general conditions. Improved estimators of the error variance components are also established.
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| 6. |
- Munthe, Christian, 1962
(författare)
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Precaution and Ethics: Handling risks, uncertainties and knowledge gaps in the regulation of new biotechnologies
- 2017
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- This volume outlines and analyses ethical issues actualized by applying a precautionary approach to the regulation of new biotechnologies. It presents a novel way of categorizing and comparing biotechnologies from a precautionary standpoint. Based on this, it addresses underlying philosophical problems regarding the ethical assessment of decision-making under uncertainty and ignorance, and discusses how risks and possible benefits of such technologies should be balanced from an ethical standpoint. It argues on conceptual and ethical grounds for a technology neutral regulation as well as for a regulation that not only checks new technologies but also requires old, inferior ones to be phased out. It demonstrates how difficult ethical issues regarding the extent and ambition of precautionary policies need to be handled by such a regulation, and presents an overarching framework for doing so.
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| 7. |
- Ahrné, Karin, et al.
(författare)
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Tillstånd och trender för arter och deras livsmiljöer – rödlistade arter i Sverige 2015
- 2015
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- 2015 års upplaga av den svenska rödlistan är den fjärde i ordningen. Den är baserad på IUCN:s rödlistningskriterier och revideras vart femte år. I rödlistan bedöms risken som enskilda arter av djur, växter och svampar löper att försvinna från Sverige. Bedömningen utförs av ArtDatabankens medarbetare i samverkan med över 100 externa experter, indelade i 14 expertkommittéer för olika organismgrupper. Under arbetet med 2015 års rödlista har tillstånd och trender bedömts för 21 600 arter och 1 318 lägre taxa (apomiktiska arter, underarter och varieteter), sammanlagt ca 22 900 taxa. Av de bedömda arterna klassificerades 2 029 som hotade (kategorierna CR, EN och VU) och 4 273 som rödlistade (inkluderar även kategorierna NT, RE och DD). Förhållandet mellan antalet rödlistade och antalet bedömda arter ar 19,8 %, vilket är ungefär samma värde som 2010 och 2005. I denna rapport jämförs antalet och andelen rödlistade arter mellan olika organismgrupper, biotoper, substrat och påverkansfaktorer. Texten ar indelad i en allmän del och åtta kapitel inriktade på olika landskapstyper. Landskapstyperna utgör en grov indelning av landets miljöer enligt följande kategorier: Skog, Jordbrukslandskap, Urbana miljöer, Fjäll, Våtmarker, Sötvatten, Havsstränder och Havsmiljöer. Skogen och jordbrukslandskapet är de artrikaste landskapstyperna med 1 800 respektive 1 400 arter som har en stark anknytning dit, och ytterligare flera hundra arter som förekommer där mer sporadiskt. De faktorer som påverkar flest rödlistade arter i Sverige är skogsavverkning och igenväxning, som båda utgör ett hot mot vardera ca 30 % av de rödlistade arterna. Avverkning minskar arealen av skog där naturliga strukturer och naturlig dynamik upprätthålls, och den orsakar därmed förlust av livsmiljöer. Igenväxning orsakas av ett antal faktorer, bland annat upphörande hävd (bete och slåtter), gödsling, trädplantering och brist på naturliga störningsregimer som t.ex. regelbundna översvämningar kring vattendrag och sjöar. Andra viktiga påverkansfaktorer är fiske, torrläggning av våtmarker, tillbakagång hos värdarter (främst alm och ask som drabbats av invasiva svampsjukdomar), klimatförändringar och konkurrens från invasiva arter. IUCN:s rödlisteindex beräknas för ett urval av de bedömda organismgrupperna. Rödlisteindex visar att skillnaderna mellan rödlistorna från 2000, 2005, 2010 och 2015 är små. Ett par undantag finns dock. Groddjur och stora däggdjur har fått en något förbättrad situation sedan 2000. Totalt förefaller det ändå som att trycket mot Sveriges artstock har förblivit relativt konstant under de senaste 15 åren.
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| 8. |
- Egeskog, Andrea, 1981, et al.
(författare)
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Actions and opinions of Brazilian farmers who shift to sugarcane : an interview-based assessment with discussion of implications for land-use change
- 2016
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Ingår i: Land use policy. - Kidlington : Elsevier. - 0264-8377 .- 1873-5754. ; 57, s. 594-604
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Tidskriftsartikel (refereegranskat)abstract
- Sugarcane ethanol systems can deliver large greenhouse gas emissions savings if emissions associated with land-use change are kept low. This qualitative study documents and analyzes actions and opinions among Brazilian farmers who shift to sugarcane production. Semi-structured interviews were held with 28 actors associated with sugarcane production in three different regions: one traditional sugarcane region and two regions where sugarcane is currently expanding. Most farmers considered sugarcane a land diversification option with relatively low economic risk, although higher risk than their previous land use. Beef production was considered a low-risk option, but less profitable than sugarcane. In conjunction with converting part of their land to sugarcane, most farmers maintained and further intensified their previous agricultural activity, often beef production. Several farmers invested in expanded production in other regions with relatively low land prices. Very few farmers in the expansion regions shifted all their land from the former, less profitable, use to sugarcane. Very few farmers in this study had deforested any land in connection with changes made when shifting to sugarcane. The respondents understand "environmental friendliness" as compliance with the relevant legislation, especially the Brazilian Forest Act, which is also a requirement for delivering sugarcane to the mills. Indirect land-use change is not a concern for the interviewed farmers, and conversion of forests and other native vegetation into sugarcane plantations is uncontroversial if legal. We derive hypotheses regarding farmers' actions and opinions from our results. These hypotheses aim to contribute to better understanding of what takes place in conjunction with expansion of sugarcane and can, when tested further, be of use in developing, e.g., policies for iLUC-free biofuel production.
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| 9. |
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| 10. |
- Messing, Ingmar
(författare)
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Land and land use : Natural preconditions
- 2011
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Ingår i: Agriculture and Forestry in Sweden since 1900- a cartographic description. - 9789187760617 ; , s. 14-22
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 11. |
- Messing, Ingmar
(författare)
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Marken i Sverige : Naturliga förutsättningar
- 2011
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Ingår i: Sveriges nationalatlas. Jordbruk och skogsbruk i Sverige sedan år 1900: en kartografisk beskrivning. - 9789187760594 ; , s. 14-22
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 12. |
- Tunon, Håkan, et al.
(författare)
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Kulturarv och hållbar utveckling
- 2010
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Ingår i: Bebyggelsehistorisk tidskrift. - : Föreningen Bebyggelsehistorisk tidskrift. - 0349-2834 .- 2002-3812. ; , s. 99–106-
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 13. |
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| 14. |
- Englund, Oskar, et al.
(författare)
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Beneficial land use change: Strategic expansion of new biomass plantations can reduce environmental impacts from EU agriculture
- 2020
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Ingår i: Global Environmental Change. - : Elsevier BV. - 0959-3780 .- 1872-9495. ; 60
-
Tidskriftsartikel (refereegranskat)abstract
- Society faces the double challenge of increasing biomass production to meet the future demands for food, materials and bioenergy, while addressing negative impacts of current (and future) land use. In the discourse, land use change (LUC) has often been considered as negative, referring to impacts of deforestation and expansion of biomass plantations. However, strategic establishment of suitable perennial production systems in agricultural landscapes can mitigate environmental impacts of current crop production, while providing biomass for the bioeconomy. Here, we explore the potential for such “beneficial LUC” in EU28. First, we map and quantify the degree of accumulated soil organic carbon losses, soil loss by wind and water erosion, nitrogen emissions to water, and recurring floods, in ∼81.000 individual landscapes in EU28. We then estimate the effectiveness in mitigating these impacts through establishment of perennial plants, in each landscape. The results indicate that there is a substantial potential for effective impact mitigation. Depending on criteria selection, 10–46% of the land used for annual crop production in EU28 is located in landscapes that could be considered priority areas for beneficial LUC. These areas are scattered all over Europe, but there are notable “hot-spots” where priority areas are concentrated, e.g., large parts of Denmark, western UK, The Po valley in Italy, and the Danube basin. While some policy developments support beneficial LUC, implementation could benefit from attempts to realize synergies between different Sustainable Development Goals, e.g., “Zero hunger”, “Clean water and sanitation”, “Affordable and Clean Energy”, “Climate Action”, and “Life on Land”.
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| 15. |
- Englund, Oskar, 1982, et al.
(författare)
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Meeting Sustainability Requirements for SRC Bioenergy: Usefulness of Existing Tools, Responsibilities of Involved Stakeholders, and Recommendations for Further Developments
- 2012
-
Ingår i: Bioenergy Research. - : Springer Science and Business Media LLC. - 1939-1234 .- 1939-1242. ; 5:3, s. 606-620
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Tidskriftsartikel (refereegranskat)abstract
- Short rotation coppice (SRC) is considered an important biomass supply option for meeting the European renewable energy targets. This paper presents an overview of existing and prospective sustainability requirements, Member State reporting obligations and parts of the methodology for calculating GHG emissions savings within the EU Renewable Energy Directive (RED), and shows how these RED-associated sustainability criteria may affect different stakeholders along SRC bioenergy supply chains. Existing and prospective tools are assessed on their usefulness in ensuring that SRC bioenergy is produced with sufficient consideration given to the RED-associated criteria. A sustainability framework is outlined that aims at (1) facilitating the development of SRC production systems that are attractive from the perspectives of all stakeholders, and (2) ensuring that the SRC production is RED eligible. Producer manuals, EIAs, and voluntary certification schemes can all be useful for ensuring RED eligibility. However, they are currently not sufficiently comprehensive, neither individually nor combined, and suggestions for how they can be more complementary are given. Geographical information systems offer opportunities for administrative authorities to provide stakeholders with maps or databases over areas/fields suitable for RED-eligible SRC cultivation. However, proper consideration of all relevant aspects requires that all stakeholders in the SRC supply chain become engaged in the development of SRC production systems and that a landscape perspective is used.
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| 16. |
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| 17. |
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| 18. |
-
Heritage as Common(s) - Common(s) as Heritage
- 2015
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Samlingsverk (redaktörskap) (övrigt vetenskapligt/konstnärligt)abstract
- The book consitutes the printed outcome of a seminar series run by the Critical Heritage Initiative (University of Gothenburg) and the Urban Heritage Cluster (Curating the City).
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| 19. |
-
Heritage as Common(s) - Common(s) as Heritage
- 2015
-
Samlingsverk (redaktörskap) (refereegranskat)abstract
- The book consitutes the printed outcome of a seminar series run by the Critical Heritage Initiative (University of Gothenburg) and the Urban Heritage Cluster (Curating the City).
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| 20. |
- Blennow, Kristina
(författare)
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Simulating wind disturbance impacts on forest landscapes: Tree-level heterogeneity matters
- 2014
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Ingår i: Environmental Modelling and Software. - : Elsevier BV. - 1364-8152 .- 1873-6726. ; 51, s. 1-11
-
Tidskriftsartikel (refereegranskat)abstract
- Wind is the most detrimental disturbance agent in Europe's forest ecosystems. In recent years, disturbance frequency and severity have been increasing at continental scale, a trend that is expected to continue under future anthropogenic climate change. Disturbance management is thus increasingly important for sustainable stewardship of forests, and requires tools to evaluate the effects of management alternatives and climatic changes on disturbance risk and ecosystem services. We here present a process-based model of wind disturbance impacts on forest ecosystems, integrated into the dynamic landscape simulation model iLand. The model operates at the level of individual trees and simulates wind disturbance events iteratively, i.e., dynamically accounting for changes in forest structure and newly created edges during the course of a storm. Both upwind gap size and local shelter from neighboring trees are considered in this regard, and critical wind speeds for uprooting and stem breakage are distinguished. The simulated disturbance size, pattern, and severity are thus emergent properties of the model. We evaluated the new simulation tool against satellite-derived data on the impact of the storm Gudrun (January 2005) on a 1391 ha forest landscape in south central Sweden. Both the overall damage percentage (observation: 21.7%, simulation: 21.4%) as well as the comparison of spatial damage patterns showed good correspondence between observations and predictions (prediction accuracy: 60.4%) if the full satellite-derived structural and spatial heterogeneity of the landscape was taken into account. Neglecting within-stand heterogeneity in forest conditions, i.e., the state-of-the-art in many stand-level risk models, resulted in a considerable underestimation of simulated damage, supporting the notion that tree-level complexity matters for assessing and modeling large-scale disturbances. A sensitivity analysis further showed that changes in wind speed and soil freezing could have potentially large impacts on disturbed area and patch size. The model presented here is available as open source. It can be used to study the effects of different silvicultural systems and future climates on wind risk, as well as to quantify the impacts of wind disturbance on ecosystem services such as carbon sequestration. It thus contributes to improving our capacity to address changing disturbance regimes in ecosystem management. (C) 2013 Elsevier Ltd. All rights reserved.
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| 21. |
- Kreuger, Jenny, et al.
(författare)
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Bekämpningsmedel i vattendrag från områden med odling av trädgårdsgrödor under 2008
- 2009
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Den nuvarande miljöövervakningen av bekämpningsmedel (växtskyddsmedel) är främst inriktad mot att undersöka eventuellt läckage av växtskyddsmedel som används inom jordbruket. Däremot saknar vi kunskap om hur dessa medel uppträder i miljön vid användning inom trädgårdsodlingen. I vissa delar av södra Sverige finns betydande områden med frilandsodling av bär, frukt och grönsaker, samt växthusanläggningar. Växthus har i vissa sammanhang betraktats som ’slutna miljöer’. Detta har gjort att man inte alltid haft samma höga krav när man bedömt risken för skadliga effekter i omgivande vatten av växtskyddsmedel som används i just växthus jämfört med användning på våra åkrar.Syftet med undersökningen var att utveckla kunskapen om eventuellt läckage av växtskyddsmedel som används inom frilandsodling av trädgårdsgrödor och i växthus till vatten. Undersökningen, som genomfördes under 2008, är den hitintills mest omfattande när det gäller antalet undersökta växtskyddsmedel i svenska vattendrag. Analyserna omfattade 126 enskilda substanser, varav 39 inte undersökts tidigare inom den nationella miljöövervakningen.I undersökningen ingick provtagning i vattendrag från sex områden med betydande odling av trädgårdsgrödor. Frilandsodling av bär förekom i avrinningsområdet i SÖ Småland, grönsaker i NÖ Skåne och frukt i SÖ Skåne. Växthusodling förekom i avrinningsområdet i V Skåne och NV Skåne. I dessa områden togs ett tiotal prover per område under perioden maj-oktober 2008. Därutöver analyserades ett tjugotal vattenprover från ett av de områden som ingår i den ordinarie miljöövervakningen (område N 34 i Halland). Ett fåtal prover togs också på överskottsvatten från växthus.Totalt påträffades 78 av de 126 substanser som ingick i undersökningen, med en spridning på mellan 24 och 44 substanser i vatten från de enskilda områdena. Flest substanser och högre halter påträffades i området med intensiv grönsaksodling i NÖ Skåne, från området i Halland och från de två områdena med växthusodling. Lägre halter och färre substanser återfanns i vatten från området i SÖ Småland med odling av jordgubbar och från området i SÖ Skåne med äppelodlingar.Riktvärdet överskreds i flertalet prov som samlades in från området i NÖ Skåne och i samtliga prov från de två växthusområdena (V Skåne och NV Skåne), med högst överskridanden i de två sistnämnda områdena. Insektsmedlet imidakloprid var den substans som oftast återfanns över riktvärdet i denna undersökning. Vissa av de substanser som påträffades i förhöjda halter i vatten från områden med växthusodling återfanns också i de prover som togs på överskottsvatten från växthus. Även substanser med en betydande användning i prydnadsväxtodling i växthus påträffades i vattendraget i V Skåne. Resultaten visar att det finns läckage av växtskyddsmedel från svenska växthus. Arbete med att identifiera möjliga riskmoment i samband med växtskyddsarbete i växthus, i syfte att minska förlusterna av växtskyddsmedel till omgivande vatten, bör därför vidareutvecklas.
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| 22. |
- Hartmann, Martin, et al.
(författare)
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Significant and persistent impact of timber harvesting on soil microbial communities in Northern coniferous forests
- 2012
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Ingår i: The ISME Journal. - : Springer Science and Business Media LLC. - 1751-7362 .- 1751-7370. ; 6:12, s. 2199-2218
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Tidskriftsartikel (refereegranskat)abstract
- Forest ecosystems have integral roles in climate stability, biodiversity and economic development. Soil stewardship is essential for sustainable forest management. Organic matter (OM) removal and soil compaction are key disturbances associated with forest harvesting, but their impacts on forest ecosystems are not well understood. Because microbiological processes regulate soil ecology and biogeochemistry, microbial community structure might serve as indicator of forest ecosystem status, revealing changes in nutrient and energy flow patterns before they have irreversible effects on long-term soil productivity. We applied massively parallel pyrosequencing of over 4.6 million ribosomal marker sequences to assess the impact of OM removal and soil compaction on bacterial and fungal communities in a field experiment replicated at six forest sites in British Columbia, Canada. More than a decade after harvesting, diversity and structure of soil bacterial and fungal communities remained significantly altered by harvesting disturbances, with individual taxonomic groups responding differentially to varied levels of the disturbances. Plant symbionts, like ectomycorrhizal fungi, and saprobic taxa, such as ascomycetes and actinomycetes, were among the most sensitive to harvesting disturbances. Given their significant ecological roles in forest development, the fate of these taxa might be critical for sustainability of forest ecosystems. Although abundant bacterial populations were ubiquitous, abundant fungal populations often revealed a patchy distribution, consistent with their higher sensitivity to the examined soil disturbances. These results establish a comprehensive inventory of bacterial and fungal community composition in northern coniferous forests and demonstrate the long-term response of their structure to key disturbances associated with forest harvesting.
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| 23. |
- Englund, Oskar, 1982
(författare)
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On Sustainability of Biomass for Energy and the Governance Thereof
- 2016
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Due to concerns about climate change, energy security, and resource scarcity, non- renewable resources are increasingly being displaced by biomass. As with most human activities, the production of biobased products can be associated with negative impacts. Primarily, this relates to the biomass supply systems, i.e., agriculture and forestry, which currently are major causes of biodiversity loss and degradation of ecosystem services. Developing sustainable production systems when transitioning from non-renewable resources to biomass is imperative. This thesis aims to clarify the meaning of sustainability in the context of biomass for bioenergy, and contribute to our understanding of how different forms of governance can promote sustainably sourced biomass for bioenergy. The thesis is based on five appended papers: Paper I analyses to what extent, where, and under what conditions oil palm for biodiesel in Brazil can be produced profitably, and what risks and opportunities that can be associated with introducing large-scale oil palm production in Brazil. Paper II lays the foundation for understanding how new biomass production can be introduced into landscapes while supporting rather than compromising the ability of the landscape to supply other ecosystem services. Paper III describes different forms of governance and shows how these can play different roles in promoting sustainable bioenergy in different countries. Paper IV focuses on how short rotation coppice production systems are affected by EU policy and how different governance forms can assist in adapting production systems to conform to the corresponding sustainability requirements. Finally, Paper V assesses how sustainability certification (private governance) addresses biodiversity conservation and contributes to our understanding of possible improvements.
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| 24. |
- Englund, Oskar, 1982, et al.
(författare)
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Large-scale deployment of grass in crop rotations as a multifunctional climate mitigation strategy
- 2023
-
Ingår i: GCB Bioenergy. - : Wiley. - 1757-1707 .- 1757-1693. ; 15:2, s. 166-184
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Tidskriftsartikel (refereegranskat)abstract
- The agriculture sector can contribute to climate change mitigation by reducing its own greenhouse gas (GHG) emissions, sequestering carbon in vegetation and soils, and providing biomass to substitute for fossil fuels and other GHG-intensive products. The sector also needs to address water, soil, and biodiversity impacts caused by historic and current practices. Emerging EU policies create incentives for cultivation of perennial plants that provide biomass along with environmental benefits. One such option, common in northern Europe, is to include grass in rotations with annual crops to provide biomass while remediating soil organic carbon (SOC) losses and other environmental impacts. Here, we apply a spatially explicit model on >81,000 sub-watersheds in EU27 + UK (Europe) to explore the effects of widespread deployment of such systems. Based on current accumulated SOC losses in individual sub-watersheds, the model identifies and quantifies suitable areas for increased grass cultivation and corresponding biomass- and protein supply, SOC sequestration, and reductions in nitrogen emissions to water as well as wind and water erosion. The model also provides information about possible flood mitigation. The results indicate a substantial climate mitigation potential, with combined annual GHG savings from soil-carbon sequestration and displacement of natural gas with biogas from grass-based biorefineries, equivalent to 13%–48% of current GHG emissions from agriculture in Europe. The environmental co-benefits are also notable, in some cases exceeding the estimated mitigation needs. Yield increases for annual crops in modified rotations mitigate the displacement effect of increasing grass cultivation. If the grass is used as feedstock in lieu of annual crops, the displacement effect can even be negative, that is, a reduced need for annual crop production elsewhere. Incentivizing widespread deployment will require supportive policy measures as well as new uses of grass biomass, for example, as feedstock for green biorefineries producing protein concentrate, biofuels, and other bio-based products.
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| 25. |
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