| 1. |
- Ekström, J., et al.
(författare)
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Increased polyamine formation in rat salivary glands by stimulation of their autonomic nerve supply
- 1985
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Ingår i: Archives of Oral Biology. - 0003-9969. ; 30:11-12, s. 773-775
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Tidskriftsartikel (refereegranskat)abstract
- Ornithine decarboxylase (ODC), a key enzyme in polyamine biosynthesis, was measured in the three major salivary glands of rats and mice. Enzyme activity and glandular putrescine content increased after continuous stimulation of the parasympathetic or sympathetic salivary nerves for 3 h. The activity was markedly reduced in animals fasted for 24 h to decrease nervous-reflex activation of the glands. Thus ODC activity may be linked with synthesis of secretory macromolecules.
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| 2. |
- Jönsson, Daniel, et al.
(författare)
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Immunocytochemical demonstration of estrogen receptor beta in human periodontal ligament cells.
- 2004
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Ingår i: Archives of Oral Biology. - 1879-1506 .- 0003-9969. ; 49:1, s. 85-88
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Tidskriftsartikel (refereegranskat)abstract
- Two transcription associated estrogen receptor (ER) subtypes have been identified and named ERα and ERβ. In the present study we investigate the expression of these ER subtypes in cultured human periodontal ligament (PDL) cells by immunocytochemistry. ERβ immunoreactivity was observed in the nuclei of about 40% of the PDL cells, while no ERα immunoreactivity was detected. In human breast cancer MCF-7 cells, serving as positive controls, both ERα and ERβ immunoreactivities were demonstrated. No immunoreactivity was observed after omission of the primary antibodies. This study suggests that estrogen acts on gene transcription preferentially via ERβ in human PDL cells.
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| 7. |
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| 8. |
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| 9. |
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| 11. |
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| 12. |
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| 13. |
- Larsson, P A, et al.
(författare)
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Inhibition of herpes simplex virus replication and protein synthesis by non-smoked tobacco, tobacco alkaloids and nitrosamines
- 1992
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Ingår i: Archives of Oral Biology. - 0003-9969 .- 1879-1506. ; 37:11, s. 969-978
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Tidskriftsartikel (refereegranskat)abstract
- Inhibitory effects of snuff extract and the tobacco chemicals nicotine, anabasine, diethyl-N-nitrosamine (DEN), and the tobacco-specific nitrosamines (TSNA), N-nitrosonornicotine (NNN) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) on herpes simplex virus type 1 (HSV-1) replication in vitro and on HSV-1 protein synthesis in infected cells were analysed. Snuff extract and nicotine caused a significant reduction of HSV-1 attachment to cell membranes whereas anabasine, DEN, NNN and NNK did not affect adsorption of HSV-1. Virus production assays in the presence of snuff added after virus adsorption resulted in a significantly reduced production of virus at low multiplicities of infection (MOI), but at high MOI the inhibitory effect of snuff extract was less pronounced. DEN, NNN and NNK only affected virus production at toxic concentrations. Nicotine and anabasine reduced virus production in non-toxic doses but not at the concentrations present in snuff extract. In HSV-infected cells exposed to snuff extract, the immediate early (α-) infected cell proteins (ICPs) 4 and 27 (as well as the early (β-) ICPs 6 and 8) were markedly increased, whereas the late (γ-) ICPs 5, 11 and 29 were reduced. Nicotine had a less pronounced stimulating effect on the production of α-proteins but no detectable effect on production of β- or -γ-proteins. Anabasine, DEN, NNN and NNK did not affect HSV protein synthesis at non-toxic concentrations. Synthesis of thymidine kinase and DNA polymerase was significantly reduced by snuff extract. Also nicotine and anabasine affected thymidine kinase and DNA polymerase but only at toxic concentrations. The production of the cellular protein actin, which almost disappears a few hours after HSV-1 infection, remained at a significant level in HSV-infected cells exposed to snuff. Thus snuff extract blocks the replicative cycle of HSV at an early stage, which results in an increased production of α-proteins in the infected cells and in prolonged maintenance of cellular functions. This may be of importance for HSV-induced transformation and the development of HSV-associated tumours.
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| 14. |
- LINDEN, LA, et al.
(författare)
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Human dentine as a hydrogel
- 1995
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Ingår i: Archives of oral biology. - : Elsevier BV. - 0003-9969. ; 40, s. 991-
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Tidskriftsartikel (refereegranskat)
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| 15. |
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| 16. |
- Ryberg, M, et al.
(författare)
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The effects of long-term treatment with salmeterol and salbutamol on the flow rate and composition of whole saliva in the rat.
- 1995
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Ingår i: Archives of Oral Biology. - 0003-9969 .- 1879-1506. ; 40:3, s. 187-191
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Tidskriftsartikel (refereegranskat)abstract
- The effect of long-acting beta 2-adrenoceptor agonists on salivary glands and saliva secretion has not been studied before. Sprague-Dawley rats were given either the long-acting beta 2-agonist salmeterol, 1 mg/kg body wt per day or the short-acting agonist salbutamol, 5 mg/kg per day. Saline solution was used as control. After 18 days pilocarpine-stimulated saliva was collected, and after 21 days saliva was collected after stimulation with isoproterol and pilocarpine in combination. The saliva was analysed for total protein, amylase, hexosamine, sialic acid, sodium, potassium and calcium. At day 25 the salivary glands were extirpated and weighed. The weight of the parotid glands increased significantly after both salmeterol and salbutamol treatment, approx. 40%; the submandibular gland weights were not affected by either beta 2-agonist treatment. Pilocarpine-stimulated salivary flow rate was increased in the salbutamol, but not in the salmeterol, group. In the salmeterol group the concentration of sialic acid was increased and that of calcium was decreased. In saliva stimulated with pilocarpine and isoproterenol in combination, the concentrations of total protein, amylase and calcium were decreased after salmeterol. In the salbutamol group, total protein and potassium were decreased. The ratio sialic acid: total protein was increased at both saliva collections in both beta 2-agonist groups. It is concluded that rats treated chronically with the long-acting beta 2-adrenoceptor agonist salmeterol have an impaired secretion of salivary proteins and calcium and that the effect resembles that of salbutamol.
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| 17. |
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| 18. |
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| 19. |
- Sonesson, Mikael, et al.
(författare)
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Minor salivary gland secretion in children and adults
- 2003
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Ingår i: Archives of Oral Biology. - : Elsevier. - 0003-9969 .- 1879-1506. ; 48:7, s. 535-539
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Tidskriftsartikel (refereegranskat)abstract
- The minor salivary glands are of great importance in the physiology and pathology of the oral cavity. So far, studies of the minor glands have concentrated on adults. In the present study, minor salivary gland secretion was studied in the buccal and labial mucosa of 3-year-old children, adolescents and young adults. In addition, the number of glands per surface area was assessed in the labial mucosa. A total of 90 individuals were included, 30 in each age-group. Saliva was collected on filter paper discs and the salivary secretion rate was measured using a Periotron 8000. The number of secreting labial glands was assessed on PAS-stained filter paper discs under a microscope. Salivary secretion in the buccal mucosa was found to be age-related, with a statistically significant lower rate of secretion (P=0.003) in the 3-year-olds (mean 7.7 microl x cm(-2) x min(-1)) compared with the young adults (11.9 microl x cm(-2) x min(-1)). No significant differences between the sexes were noted. For the labial glands, no age- or sex-related differences were found. In all age-groups, salivary secretion was significantly higher in the buccal than in the labial mucosal area. A statistically significant difference in number of secreting glands was found between all age-groups, with a decreasing number of glands per surface unit with age. The number of glands was significantly lower in males compared with females in the group of adults. The lower rate of buccal salivary secretion in the young children may imply that the oral mucosa is more vulnerable to external injury and that caries protection on the buccal molar surfaces is lower. Previous studies indicate that adults with a reduced rate of minor salivary gland secretion are more susceptible to caries.
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| 20. |
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| 21. |
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| 22. |
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| 23. |
- Almståhl, Annica, 1973, et al.
(författare)
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Acid-producing capacity from sugars and sugar alcohols among Lactobacillus isolates collected in connection with radiation therapy
- 2017
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Ingår i: Archives of Oral Biology. - : Elsevier BV. - 0003-9969. ; 84, s. 82-88
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Tidskriftsartikel (refereegranskat)abstract
- Objective To investigate the acid-producing capacity from sugars and sugar alcohols of oral Lactobacillus collected in connection with radiation therapy (RT) to the head and neck region. Design Lactobacillus were collected from the tongue, buccal mucosa and supragingival plaque in 24 patients before, during, and after RT. The acid-producing capacity of Lactobacillus isolates (n = 211) was analyzed using a colorimetric fermentation test in microtiter plates. Solutions containing 2% sugars (sucrose, glucose, fructose, lactose) or sugar-alcohols (sorbitol and xylitol) were used. After 24 h of incubation, bacterial acid-producing capacity was determined as strong (pH < 5), weak (pH ≥5–≤ 6) or low/absent (pH > 6). Data regarding intake frequency of sugar-rich products and products with sugar-alcohols was collected. Results The highest acid-producing capacity using the sugars was seen for isolates collected during RT. Sorbitol was fermented to a higher extent during and post RT, especially among isolates from plaque. Lactobacillus fermenting xylitol showed the highest acid-producing capacity during RT (p < 0.05). No statistically significant correlations between stimulated whole salivary secretion rate and acid-producing capacity, or between the intake frequency of sugar-rich products or sugar-alcohol containing products and Lactobacillus acid-producing capacity, were found. Conclusion The results suggest that Lactobacillus isolates, collected from the tongue, buccal mucosa and supragingival plaque, have a higher acid-producing capacity using sugars and sugar-alcohols during RT than one year post RT. © 2017 Elsevier Ltd
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| 24. |
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| 25. |
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| 26. |
- Almståhl, Annica, 1973, et al.
(författare)
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Microflora in oral ecosystems and salivary secretion rates - A 3-year follow-up after radiation therapy to the head and neck region
- 2015
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Ingår i: Archives of Oral Biology. - : Elsevier BV. - 0003-9969 .- 1879-1506. ; 60:9, s. 1187-1195
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Tidskriftsartikel (refereegranskat)abstract
- Objective: Results indicate that late improvements of radiation therapy (RT) in the head and neck region may diminish the long-term effects on salivary glands and oral microflora. The aim was therefore to analyze salivary secretion rates and oral microflora over time in RT subjects. Design: Twelve dentate subjects (28 +/- 2 teeth) and 12 controls were included. A clinical examination was performed and the salivary secretion rates were determined. Microbial samples, analyzed using cultivation technique, were collected from the soft tissues, supragingival plaque and gingival crevice region. Results: Compared with the controls, the RT group (n = 11) had 3 years post RT higher numbers and proportions of lactobacilli (p < 0.001 and p < 0.01) and Candida albicans (p < 0.01 and p < 0.05) in the supragingival plaque, higher numbers of enterococci in the vestibulum in the molar region and on the tongue (p < 0.05 for both), a lower total count (p < 0.001) and lower numbers of streptococci, Streptococcus salivarius and Fusobacterium nucleatum (p < 0.01) on the tongue. Although both stimulated and unstimulated salivary secretion rates were increased over time, the proportion of microorganisms associated with oral health decreased, and microorganisms associated with oral disorders increased. Despite a comparable oral hygiene, it was only the 27% who had a stimulated salivary secretion rate >= 1.0 ml/min and a buffering capacity >= 6.0, where a recovery of the flora could be seen. Conclusion: The results indicate that regaining a normal, stimulated salivary secretion rate and buffering capacity are prerequisites to regaining an oral flora associated with good oral health. (C) 2015 Elsevier Ltd. All rights reserved.
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| 27. |
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| 28. |
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| 29. |
- Cabras, Tiziana, et al.
(författare)
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Carbachol-induced in vitro secretion of certain human submandibular proteins investigated by mass-spectrometry.
- 2008
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Ingår i: Archives of oral biology. - : Elsevier BV. - 1879-1506 .- 0003-9969. ; 53:11, s. 1077-83
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: To investigate protein content of saliva produced in vitro by samples of human submandibular gland following stimulation with the muscarinic agent carbachol. DESIGN: Tissue samples, obtained at surgery from seven patients and showing normal morphological appearance, were tested for 30 min: in absence of carbachol and atropine; in presence of carbachol (10 microM); in presence of carbachol (10 microM) and atropine (20 microM); or in presence of just atropine (20 microM). Medium was analysed by high-performance liquid chromatography-mass-spectrometry. Neither before nor during surgery were the patients exposed to drug treatments that were likely to influence the in vitro secretion. RESULTS: Proline-rich proteins (PRP)-1 and -3, peptide PC and PB, statherin, cystatins SN, S1 and S2 were invariably found in control gland tissue medium. Mean concentrations of these proteins/peptides in the medium were non-proportionally elevated following carbachol exposure to the gland tissues. Difference between basal release and carbachol-induced secretion achieved statistical significance as to all the proteins/peptides under study but for statherin. Atropine alone or atropine plus carbachol caused no significant changes compared to the basal release of proteins/peptides. CONCLUSIONS: In vitro studies on salivary glands make it possible to study protein secretion from individual glands and thus, to reveal the contribution of the various types of gland to protein/peptide content of whole saliva. The disproportional responses to carbachol may imply that the proteins/peptides are not confined to the same cells or to the same intracellular locations and are therefore not secreted as packages at parasympathetic cholinergic activity.
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| 30. |
- Cabras, T., et al.
(författare)
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Proteomics of the acid-soluble fraction of whole and major gland saliva in burning mouth syndrome patients
- 2019
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Ingår i: Archives of Oral Biology. - : Elsevier BV. - 0003-9969. ; 98, s. 148-155
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Tidskriftsartikel (refereegranskat)abstract
- Objective: In the present study the salivary proteome of burning mouth syndrome patients and healthy subjects was characterized by a top-down proteomic approach and compared to highlight possible qualitative and quantitative differences that may give suggestions about the causes of this pathology which are still unknown. Materials and methods: Resting and stimulated whole saliva, stimulated parotid and submandibular/sublingual saliva samples were collected from burning mouth syndrome patients (n = 16) and age- and gender-matched healthy subjects (n = 14). An equal volume of 0.2% trifluoroacetic acid was added to each sample immediately after collection and the supernatants were analysed by liquid chromatography coupled to electrospray-ionisation mass spectrometry. Proteins and peptides were quantified using a label-free approach measuring the extracted ion current peak areas of the main salivary proteins and peptides. Results: The quantitation of the main salivary proteins and peptides revealed a higher concentration of cystatin SN in resting saliva of burning mouth syndrome patients with respect to healthy controls and no other conspicuous changes. Conclusions: The reported data showed that the salivary protein profile was not affected, in composition and relative abundance, by the burning mouth syndrome, except for the cystatin SN, a protein up-regulated in several pathological conditions, that might be considered potentially indicative of the disease. © 2018
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| 31. |
- Carlén, Anette, 1950, et al.
(författare)
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Human minor and major gland saliva proteins and ability to mediate Actinomyces naeslundii adherence.
- 2004
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Ingår i: Archives of oral biology. - : Elsevier BV. - 0003-9969. ; 49:3, s. 177-81
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Tidskriftsartikel (refereegranskat)abstract
- Bacteria-binding components and the ability to mediate bacterial adhesion to the tooth surface have been thoroughly studied in major salivary gland secretions. Our knowledge on the bacteria binding activity in minor gland saliva is, however, limited. In this study, proteins were examined in parallel in minor (palatal, buccal and labial) and major (parotid and submandibular/sublingual) salivary gland secretions in one subject using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The adherence of early colonizing Actinomyces naeslundii to pellicles formed from the secretions on hydroxyapatite beads was also examined. Amylase, IgA, proline-rich proteins and the high-molecular-weight glycoproteins, agglutinins, were detected in all saliva tested. Carbohydrate-reactive antibodies recognized the low-molecular weight mucin, MUC 7 in submandibular/sublingual saliva only. A. naeslundii strain 12104 adhered to all pellicles and especially to the buccal gland saliva pellicles. Strain LY7 adhered in highest numbers to the submandibular/sublingual saliva pellicles. It also bound in considerable numbers to parotid and palatal saliva pellicles but not to the ones formed from buccal and labial gland saliva. Our findings indicate that several bacteria-binding components are secreted in both minor and major gland saliva. The adherence-promoting ability of the various gland secretions differs, however.
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| 32. |
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| 33. |
- Darnell, Malin, et al.
(författare)
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Lipopolysaccharide induced-in vivo increases in beta-defensins of the rat parotid gland.
- 2006
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Ingår i: Archives of oral biology. - : Elsevier BV. - 0003-9969. ; 51:9, s. 769-74
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Tidskriftsartikel (refereegranskat)abstract
- Antimicrobial beta-defensins are thought to protect epithelial surfaces. Their mobilization in response to inflammation was studied in the rat parotid gland using an ELISA assay. Bacterial lipopolysaccharide (LPS), injected into the parotid duct on one side, induced a marked local inflammatory response in the parotid gland as judged by several fold increases in myeloperoxidase activity and, in histological sections, infiltration of neutrophils. Three hours after the injection, beta-defensin 1 and 3 were increased (by 41% and 15%, respectively, P<0.01) as compared to the contralateral gland. Though still elevated 6h after the injection, the percentage figures for beta-defensin 1 were, at this time, somewhat lower (30%) compared to the situation at 3h, while those for defensin 3 were significantly higher 65% (P<0.01); neither at the early nor at the late time of observation were any changes in the level of beta-defensin 2 observed. The beta-defensins under study were not detected in submandibular and sublingual glands, neither were they detected in the inflamed submandibular gland, showing also here several fold increases in myeloperoxidase activity and, in addition, the presence of inflammatory cells, following ductal injection of LPS towards the gland.
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| 34. |
- Davis, G. R., et al.
(författare)
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Microscopic study of dental hard tissues in primary teeth with Dentinogenesis Imperfecta Type II: Correlation of 3D imaging using X-ray microtomography and polarising microscopy
- 2015
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Ingår i: Archives of Oral Biology. - : Elsevier BV. - 0003-9969. ; 60:7, s. 1013-1020
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Tidskriftsartikel (refereegranskat)abstract
- Objectives: The aim of this study was to examine the histological appearance of dental hard tissues in primary teeth from children with DI using conventional polarised light microscopy and correlate that with 3D imaging using X-ray microtomograpy (XMT) to gain a further understanding of the dentine structure of teeth diagnosed with dentinogenesis imperfecta. Design: Undecalcified sections of primary teeth from patients diagnosed with Dentinogenesis Imperfecta Type II were examined using polarised light microscopy. XMT was employed for 3D-imaging and analysis of the dentine. Results: The polarised light microscopy and XMT revealed tubular structures in the dentine seen as vacuoles coinciding with the path of normal dentinal tubules but not continuous tubules. The size of the tubules was close to that of capillaries. The largest tubular structures had a direction corresponding to where the pulp tissue would have been located during primary dentine formation. The dysfunctional mineralisation of the dentine and obliteration of the pulp evidently leaves blood vessels in the dentine which have in the main been tied off and, in the undecalcified sections, appear as vacuoles. Conclusions: Although from radiographs, the pulp in teeth affected by Dentinogenesis Imperfect type II appears to be completely obliterated, a network of interconnected vessels may remain. The presence of large dentinal tubules and blood vessels, or the remnants of blood vessels, could provide a pathway for bacteria from the oral cavity. This might account for why some of these teeth develop periapical abscesses in spite of apparently having no pulp. (c) 2015 Elsevier Ltd. All rights reserved.
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| 35. |
- Dawes, C., et al.
(författare)
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The functions of human saliva: A review sponsored by the World Workshop on Oral Medicine VI
- 2015
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Ingår i: Archives of Oral Biology. - : Elsevier BV. - 0003-9969. ; 60:6, s. 863-874
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Forskningsöversikt (refereegranskat)abstract
- This narrative review of the functions of saliva was conducted in the PubMed, Embase and Web of Science databases. Additional references relevant to the topic were used, as our key words did not generate references which covered all known functions of saliva. These functions include maintaining a moist oral mucosa which is less susceptible to abrasion, and removal of micro-organisms, desquamated epithelial cells, leucocytes and food debris by swallowing. The mucins form a slimy coating on all surfaces in the mouth and act as a lubricant during such processes as mastication, formation of a food bolus, swallowing and speaking. Saliva provides the fluid in which solid tastants may dissolve and distributes tastants around the mouth to the locations of the taste buds. The hypotonic unstimulated saliva facilitates taste recognition. Salivary amylase is involved in digestion of starches. Saliva acts as a buffer to protect oral, pharyngeal and oesophageal mucosae from orally ingested acid or acid regurgitated from the stomach. Saliva protects the teeth against acid by contributing to the acquired enamel pellicle, which forms a renewable lubricant between opposing tooth surfaces, by being supersaturated with respect to tooth mineral, by containing bicarbonate as a buffer and urea and by facilitating clearance of acidic materials from the mouth. Saliva contains many antibacterial, antiviral and antifungal agents which modulate the oral microbial flora in different ways. Saliva also facilitates the healing of oral wounds. Clearly, saliva has many functions which are needed for proper protection and functioning of the human body. (C) 2015 Elsevier Ltd. All rights reserved. RAMS CK, 1988, GASTROENTEROLOGY, V95, P1460
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| 36. |
- Dohan Ehrenfest, David, et al.
(författare)
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Choukroun's platelet-rich fibrin (PRF) stimulates in vitro proliferation and differentiation of human oral bone mesenchymal stem cell in a dose-dependent way.
- 2010
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Ingår i: Archives of oral biology. - : Elsevier BV. - 1879-1506 .- 0003-9969. ; 55:3, s. 185-94
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Choukroun's platelet-rich fibrin (PRF) is an autologous leukocyte- and platelet-rich fibrin biomaterial. The purpose of this study was to analyse the in vitro effects of PRF on human bone mesenchymal stem cells (BMSC), harvested in the oral cavity after preimplant endosteal stimulation. MATERIALS AND METHODS: BMSCs from primary cultures were cultivated with or without a PRF membrane originating from the same donor as for the cells, in proliferation or osteoblastic differentiation conditions. After 7 days, the PRF membranes were removed. A series of cultures were performed using 2 PRF membranes, in order to measure the dose-dependent effect. Cell counts, cytotoxicity tests, alkaline phosphatase (ALP) activity quantification, Von Kossa staining and mineralisation nodules counts were performed at 3, 7, 14, 21 and 28 days. A last independent series was carried on up to 14 days, for a morphological scanning electron microscope (SEM) observation. RESULTS: PRF generated a significant stimulation of the BMSC proliferation and differentiation throughout the experimental period. This effect was dose-dependent during the first weeks in normal conditions, and during the whole experimentation in differentiation conditions. The cultures without PRF in differentiation conditions did not rise above the degree of differentiation of the cultures in normal conditions with 1 or 2 PRF up to the 14th and 28th day, respectively. The SEM culture analysis at day 14 allowed to show the mineralisation nodules which were more numerous and more structured in the groups with PRF compared to the control groups. DISCUSSION AND CONCLUSIONS: This double contradictory proliferation/differentiation result may be due to the numerous components of PRF, particularly the presence of leukocytes: any culture with PRF is in fact a coculture with leukocytes. It could be the source of differential geographic regulation processes within the culture. The combination of oral BMSC and PRF might offer many potential clinical and biotechnological applications, and deserves new studies.
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| 37. |
- Eick, Sigrun, et al.
(författare)
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Adhesion of Porphyromonas gingivalis and Tannerella forsythia to dentin and titanium with sandblasted and acid etched surface coated with serum and serum proteins - An in vitro study
- 2017
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Ingår i: Archives of Oral Biology. - : Elsevier. - 0003-9969 .- 1879-1506. ; 75, s. 81-88
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Tidskriftsartikel (refereegranskat)abstract
- Objective: To evaluate the adhesion of selected bacterial strains incl. expression of important virulence factors at dentin and titanium SLA surfaces coated with layers of serum proteins. Methods: Dentin-and moderately rough SLA titanium-discs were coated overnight with human serum, or IgG, or human serum albumin (HSA). Thereafter, Porphyromonas gingivalis, Tannerella forsythia, or a six species mixture were added for 4 h and 24 h. The number of adhered bacteria (colony forming units; CFU) was determined. Arg-gingipain activity of P. gingivalis and mRNA expressions of P. gingivalis and T forsythia proteases and T. forsythia protease inhibitor were measured. Results: Coating specimens never resulted in differences exceeding 1.1 log10 CFU, comparing to controls, irrespective the substrate. Counts of T forsythia were statistically significantly higher at titanium than dentin, the difference was up to 3.7 log10 CFU after 24 h (p = 0.002). No statistically significant variation regarding adhesion of the mixed culture was detected between surfaces or among coatings. Arggingipain activity of P. gingivalis was associated with 1og10 CFU but not with the surface or the coating. Titanium negatively influenced mRNA expression of T. forsythia protease inhibitor at 24 h (p = 0.026 uncoated, p = 0.009 with serum). Conclusions: The present findings indicate that: a) single bacterial species (T forsythia) can adhere more readily to titanium SLA than to dentin, b) low expression of T. forsythia protease inhibitor may influence the virulence of the species on titanium SLA surfaces in comparison with teeth, and c) surface properties (e.g. material and/or protein layers) do not appear to significantly influence multi-species adhesion. (C) 2016 Elsevier Ltd. All rights reserved.
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| 38. |
- Ekström, Jörgen, et al.
(författare)
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Nitric oxide-dependent mitotic activity in salivary glands of the rat upon sympathetic stimulation
- 2004
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Ingår i: Archives of Oral Biology. - : Elsevier. - 0003-9969 .- 1879-1506. ; 49:11, s. 889-894
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Tidskriftsartikel (refereegranskat)abstract
- Incorporation of [3H]thymidine into trichloroacetic acid (TCA)-insoluble material of the parotid and submandibular glands was used as an index of mitotic activity following unilateral electrical stimulation of the sympathetic innervation (20 Hz, 4 min every fifth minute over 34 min). Stimulation under β-adrenoceptor blockade (propranolol 2 mg/kg, intravenous) alone or combined with α-adrenoceptor blockade (phentolamine 2 mg/kg, intravenous) did not increase the rate of [3H]thymidine incorporation into the two types of glands. However, under α-adrenoceptor blockade the [3H]thymidine incorporation increased into the parotid glands, by 122% (compared to the glands on the contralateral side), but not into the submandibular glands. In the presence of the neuronal type NO-synthase (nNOS) blocker N-PLA (30 mg/kg, intravenous) or the unselective NO-synthase blocker L-NAME (30 mg/kg, intravenous), this increase was reduced to 49 and 47%, respectively. Thus, the major part of the sympathetically nerve-evoked β-adrenoceptor-mediated mitotic response was found to depend on the activity of neuronal type NO-synthase to generate NO. Since the sympathetic nerve fibres of the parotid gland lack NO-synthase, the neuronal type NO-synthase subjected to the inhibitors is likely to be of parenchymal origin. © 2004 Elsevier Ltd. All rights reserved.
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| 39. |
- Eliasson, Lars, 1940, et al.
(författare)
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Feeling of dry mouth in relation to whole and minor gland saliva secretion rate.
- 2009
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Ingår i: Archives of oral biology. - : Elsevier BV. - 1879-1506 .- 0003-9969. ; 54:3, s. 263-7
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Tidskriftsartikel (refereegranskat)abstract
- Previous reports indicate an association between whole saliva secretion rate and xerostomia, but the association is not always found in individuals complaining of dry mouth. In the present study, we hypothesised that the minor salivary gland secretions could affect subjective feelings of oral dryness in individuals with normal or low whole saliva secretion rate. 142 individuals, aged 18-82 years, participated in the study. Feelings of oral dryness were assessed separately for day- and nighttime by visual analogue scales. Resting and stimulated whole saliva flow rates were measured by conventional methods, and minor palatal, buccal and labial saliva secretion rates by the Periotron method. Twenty-three percent of the women and 15% of the men reported feelings of dry mouth during day and night. The results showed statistically significant lower resting and stimulated whole and minor labial gland saliva secretion rates in subjects reporting oral dryness during both day and night compared with non-complainers. In groups of individuals with normal simulated whole saliva secretion, those who complained from dry mouth both day and night had statistically significant lower labial secretion rate compared with those who had no complaints. Although not statistically significant, this difference was seen also in the groups with fewer individuals who had low whole saliva flow. In summary, our findings suggested that the labial gland saliva might affect subjective feelings of dry mouth both in individuals with normal and subnormal whole saliva flow.
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| 40. |
- Eliasson, Lars, 1940, et al.
(författare)
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Minor gland saliva flow rate and proteins in subjects with hyposalivation due to Sjogren's syndrome and radiation therapy.
- 2005
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Ingår i: Archives of oral biology. - : Elsevier BV. - 0003-9969. ; 50:3, s. 293-9
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Tidskriftsartikel (refereegranskat)abstract
- In this study, the secretion rate and IgA, albumin and lactoferrin concentrations in minor labial and buccal gland saliva were investigated in individuals with hyposalivation due to primary Sjogren's syndrome (pSS; 10 subjects) or head and neck radiation therapy (RT; 10 subjects) and in their matched controls. Whole saliva was similarly examined. The minor gland saliva flow was measured using the Periotron method. IgA, albumin and lactoferrin concentrations were analysed by ELISA techniques. A general finding was that the flow rate and protein concentrations were lower in labial than in buccal gland saliva. In both hyposalivation groups, the labial minor gland saliva secretion rate was lowered compared to their respective controls. The buccal gland saliva flow rate was significantly reduced in the RT group only. IgA and albumin concentrations were not different from the controls in the labial secretions. The concentration of lactoferrin was increased in the RT group. In buccal saliva, the concentrations of all proteins examined but pSS IgA, were increased compared to the controls. Reduced flow rate and increased protein concentrations were seen for whole saliva where the lactoferrin concentration was higher in RT than in pSS subjects. Thus, our findings suggested that minor gland saliva flow rate and protein concentrations are affected in RT and pSS subjects and to highest extent in the former.
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| 41. |
- Eriksson, Per-Olof, et al.
(författare)
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Instant reduction in postural sway during quiet standing by intraoral dental appliance in patients with Whiplash associated Disorders and non-trauma neck pain
- 2019
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Ingår i: Archives of Oral Biology. - : PERGAMON-ELSEVIER SCIENCE LTD. - 0003-9969 .- 1879-1506. ; 97, s. 109-115
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Tidskriftsartikel (refereegranskat)abstract
- Objectives: This study tested the hypothesis that modulation of jaw sensorimotor control by intraoral dental appliance can reduce postural sway during quiet standing and hence improve standing balance, in patients with whiplash associated disorders (WAD) and non-trauma neck pain. Design: Postural sway during quiet standing with feet together was examined in 54 WAD patients (40 females) and 10 non-trauma patients (8 females) using wireless 3D movement recording technique. Recordings were performed alternating without and with intraoral dental appliance, and with closed eyes and open eyes, respectively. In this protocol the participants served as their own controls. A reference group of 30 healthy subjects (17 females) was also recorded. Each recording lasted 120 s, followed by 3-5 min of rest. Speed, acceleration and perimeter of postural sway area were documented. Results: In the patients, but not in the healthy group, the intraoral dental appliance instantly and significantly reduced standing postural sway in recordings with closed and open eyes. Conclusions: The prompt reduction in standing postural sway from intervention by intraoral dental appliance i.e. improved standing balance, suggests a potent effect on the postural control system by modulation of the jaw sensorimotor system, probably involving reflex transmission. The result opens for new insight into mechanisms behind postural control and the pathophysiology of balance disorders, and adds to the knowledge on plasticity of the nervous system. It may help developing new procedures for assessment and management of impaired balance in WAD and non-trauma neck pain patients.
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| 42. |
- Eriksson, Per-Olof, et al.
(författare)
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Jaw-neck dysfunction in whiplash-associated disorders
- 2007
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Ingår i: Archives of Oral Biology. - : Elsevier BV. - 0003-9969 .- 1879-1506. ; 52:4, s. 404-408
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Tidskriftsartikel (refereegranskat)abstract
- This paper reports data from recent studies on integrative jaw-neck motor control in healthy subjects and disturbed jaw-neck behaviour in whiplash-associated disorders (WAD). The results show that neck function is an integral part of natural jaw behaviour, and that neck injury can impair jaw function and therefore disturb eating behaviour. We also show preliminary results from implementation of a new approach for rehabilitation of jaw-neck dysfunction and pain in WAD.
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| 43. |
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| 44. |
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| 45. |
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| 46. |
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| 47. |
- Götrick, Bengt, et al.
(författare)
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The xerogenic potency and mechanism of action of tramadol inhibition of salivary secretion in rats.
- 2004
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Ingår i: Archives of oral biology. - : Elsevier BV. - 0003-9969 .- 1879-1506. ; 49:12, s. 969-73
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Tidskriftsartikel (refereegranskat)abstract
- Tramadol is a centrally acting analgesic with weak opioid agonist properties, which also has monoaminergic activity, exerted via inhibition of neuronal uptake of serotonin and norepinephrine. Tramadol is generally well tolerated and the most common adverse events are nausea, dizziness, drowsiness, sweating, vomiting and dry mouth. Currently it was examined by which principal mechanism tramadol induces oral dryness. The effects of intravenous administration (+/-)-tramadol were studied in rats on the flow of saliva in response to a peripheral cholinergic stimulus or to reflex activation involving the relay of impulses in the central nervous system. In pentobarbitone-anaesthetized rats, the salivary secretion to acetylcholine (0.1-10 micromol/kg IV) was increased by up to 110% by tramadol (1-5 mg/kg IV) and the protein concentration therein by up to 400%. The administration alpha- and beta-adrenoceptor antagonists resulted in almost identical acetylcholine-evoked responses as in the absence of tramadol. The secretory response to the application of citric acid on the tongue of the rat was reduced by 38% and by 64%, respectively, at 5 and 10 mg/kg IV of tramadol (p < 0.05-0.01). Thus, tramadol exerts its principal xerogenic effect by activating inhibitory pathways in the central nervous system and has no anticholinergic effect on the salivary glands at dosages that may be clinically relevant. Furthermore, the tramadol-induced increase of the acetylcholine-evoked secretion occurred at a glandular level and depended most likely on a release of noradrenaline from glandular nerve terminals.
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| 48. |
- He, Tailun, 1969, et al.
(författare)
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Craniofacial growth in the ferret (Mustela putorius furo)--a cephalometric study.
- 2004
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Ingår i: Archives of oral biology. - : Elsevier BV. - 0003-9969. ; 49:10, s. 837-48
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: When suggesting the ferret as a valid laboratory model in craniofacial research, it is essential to know about its normal craniofacial growth. DESIGN: Sixteen ferret kits (eight male and eight female) were selected for the present investigation. Serial lateral and dorsoventral cephalograms were taken on each animal at a mean age of 25, 35, 55, 80 and 300 days. The cephalograms were then digitised and the coordinates of 33 landmarks were derived on each set of cephalograms. Thirty-four variables were then calculated on each set of cephalograms by computer image programs with the coordinate data. Results were analysed statistically, and the craniofacial growth pattern and related sexual dimorphism were described in three perspectives: lateral and dorsoventral viscero- and neurocranium, and lateral mandible. FINDINGS: In both sexes, the viscero- and neurocranium follow an orderly pattern of expansive growth in three dimensions. The growth of the mandible is mainly characterised by an anteroposterior elongation of the mandibular body, an enlargement of the coronoid process, and an increase in height of the alveolar process. The growth rate varies with site. Craniofacial growth in ferrets starts to slow down and finally ceases earlier in female than in male animals.
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| 49. |
- He, Tailun, 1969, et al.
(författare)
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Dental eruption and exfoliation chronology in the ferret (Mustela putorius furo).
- 2002
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Ingår i: Archives of oral biology. - 0003-9969. ; 47:8, s. 619-23
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Tidskriftsartikel (refereegranskat)abstract
- Substituting ferrets for rats and dogs as animal models for craniofacial research is favourable because of the similarity of many of the ferret's anatomical, metabolic and physiological features to those of man. Other advantages are cost-effectiveness and possibly less ethical controversy. However, information on the dental chronology of ferrets needs to be supplemented if this animal is to be promoted as an alternative model. Dental development was here examined in 16 ferrets (eight males, eight females) from three litters at between 12 and 90 days of age. Dental eruption and exfoliation were assessed and recorded every second day. The sequence of eruption of deciduous and permanent teeth was determined and data were analysed statistically. Also, any sex-related differences in eruption and exfoliation ages were defined. No deciduous incisors were observed to erupt in this group of animals. Other deciduous teeth erupted between the 19th and 31st postnatal days, and exfoliated between days 51 and 76. The time of eruption of the permanent teeth ranged from 42 to 77 days, in accordance with the stage of the mixed dentition. The female ferrets were generally ahead of the males in the exfoliation age of their deciduous teeth and the eruption age of their permanent teeth, but this, a sex difference did not apply to the eruption age of the deciduous teeth. These extended basic data might facilitate the introduction of this alternative experimental animal into craniofacial research.
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| 50. |
- He, Tailun, 1969, et al.
(författare)
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Functional influence of masticatory muscles on the fibre characteristics and capillary distribution in growing ferrets (Mustela putonusfuro)--a histochemical analysis.
- 2004
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Ingår i: Archives of oral biology. - : Elsevier BV. - 0003-9969. ; 49:12, s. 983-9
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: Changes in the masticatory function of ferrets have been shown to cause alterations in their craniofacial morphology. The aim of the present study was to investigate how the functional changes influence the histochemical characteristics of fibres of the masticatory muscles of the animal. DESIGN: Forty five-week old male ferrets were divided into two groups: one group was fed a diet of hard pellets (HD group), and one group was fed the same diet but softened with water (SD group). After 6 months, specimens of temporalis, masseter and digastricus were taken from each ferret and prepared for enzyme histochemical analysis RESULTS: Type I muscle fibres constituted the majority of the temporalis and the masseter, while the digastricus mainly comprised type II muscle fibres. The fibre-type composition did not differ significantly between the two groups. The mean cross-sectional area of type I and type II fibres in the temporalis and the masseter, and type II fibres in the digastricus was significantly greater in the HD group than the SD group. Furthermore, the HD group had a significantly greater number of capillaries per fibre, but not per surface unit (mm2). CONCLUSIONS: The results suggest that the muscle fibre size and the number of capillaries per fibre of the masticatory muscles are readily adaptesd to the altered masticatory muscle functions.
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