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| 2. |
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| 3. |
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| 4. |
- Andersson, Håkan S., 1967-, et al.
(författare)
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The toxicity of ribbon worms: alpha-nemertides or tetrodotoxin, or both?
- 2016
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Ingår i: Planta Medica. - : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221. ; 82:Supplement 1
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The marine ribbon worms (nemerteans) are predators which capture their prey by everting a proboscis carrying a mixture of toxins which brings on rapid paralysis [1]. Moreover, ribbon worms have a thick layer of epidermal mucus of similar constitution. Tetrodotoxin (TTX) has been identified as one of these toxins [2]. The extreme toxicity of TTX (lethal by ingestion of 0.5-2 mg) is due to its ability to block voltage-gated sodium channels. Although several bacterial species (among these Vibrio sp.) have been linked to its synthesis, the biogenic origin and biosynthesis is unclear. One hypothesis is that TTX production occurs in a symbiotic relationship with its host, in this case the ribbon worm [3]. We have made significant effort to identify TTX in a setup for production through the cultivation of Vibrio alginolyticus in nutrient broth infused with mucus from the ribbon worm Lineus longissimus. Toxicity was demonstrated by fraction injections into shore crabs, but no TTX was found, and it could be shown conclusively that toxicity was unrelated to TTX and the Vibrio culture itself, and rather a constituent of the ribbon worm mucus [4]. The following studies led us to the discovery of a new class of peptides, the alpha-nemertides, in the mucus of the ribbon worms, which could be directly linked to the toxic effects. A literature review of the available evidence for TTX in ribbon worms show that the evidence in most cases are indirect, although notable exceptions exist. This points to the necessity to further investigate the presence and roles of TTX and alpha-nemertides in ribbon worms.
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| 5. |
- Andersson, Maria, et al.
(författare)
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Minor components with smooth muscle relaxing properties from scented myrrh (Commiphora guidotti)
- 1997
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Ingår i: Planta Medica. - : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221. ; 63:3, s. 251-254
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Tidskriftsartikel (refereegranskat)abstract
- All sesquiterpenes present in a sample of scented myrrh were isolated and characterised. Seven compounds, with cadinane, guaiane, oplopane, and eudesmane skeletons, were obtained, of which two are new and two are reported from a natural source for the first time. The major component, T-cadinol, has previously been shown to possess smooth muscle-relaxing properties, and the major purpose of the investigation was to compare the effects of the minor and more polar sesquiterpenes with that of T-cadinol in the rat aorta. Like T-cadinol, the minor sesquiterpenes are more efficient in reducing K(+)-induced contractions than those induced by the alpha-adrenoceptor agonist phenylephrine, however, they were all less potent than T-cadinol.
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| 11. |
- Backlund, Anders, 1965-
(författare)
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Phylogeny and chemography
- 2008
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Ingår i: Planta Medica. - : Thieme Medical Publishers. - 0032-0943 .- 1439-0221. ; 74:9, s. SL64-
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Tidskriftsartikel (refereegranskat)abstract
- Natural compounds are evolutionary selected and pre-validated by Nature, displaying a unique diversity of chemical properties and corresponding biological activities. Of utmost importance for a rational discovery and exploration of new biologically active compounds are two aspects: one the identification and charting of the biologically relevant chemical space, the other a similar charting of the corresponding evolutionary space. he first key to this is the coverage of the natural products' chemical space. For this purpose we introduced ChemGPS-NP, with the aim to provide a tool for more efficient and stringent compound comparison, to identify parts of chemical space related to particular biological activities, and to track changes in chemical properties due to e.g. evolutionary traits and modifications in biosynthesis. Physical-chemical properties not directly discernible from structural data can be compared, making selection more rational when screening natural compounds and analogues. The second key would consequently be to explore evolutionary space by elucidating and utilising robust phylogenies for the organisms under study. From this basis reflecting the evolutionary history and hence biosynthesis development, further conclusions can be drawn. Based these initial attempts, the intersection of chemical and evolutionary space have been explored. With regard to e.g. compound classes such as iridoids, betalains, and sesquiterpene lactones, evolutionary patterns of changes in physical-chemical properties are observed and compared. For eight major classes of plant defence peptides analyses of structure base alignments provide arguments for rational classification. In addition, evolution of the enzyme Rubisco, have been explored with reference to major structural features.
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| 12. |
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| 13. |
- Blomkvist, Josefin, et al.
(författare)
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Perspective on Roseroot (Rhodiola rosea) Studies
- 2009
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Ingår i: Planta Medica. - : Verlag KG Stuttgart -New York. - 0032-0943 .- 1439-0221. ; 75:11, s. 1187-1190
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Tidskriftsartikel (refereegranskat)abstract
- Rhodiola rosea (roseroot) extract is a commercially successful product, primarily used to reduce the effect of fatigue on physical and mental performance. In this perspective we present our investigation of the most recent studies performed on human subjects. With a focus on the statistical methods we found considerable shortcomings in all but one of the studies that claim significant improvement from roseroot extract. Overall, the study designs have not been well explained. Experimental results have been confused and appear to be in some cases incorrect. Some of the conclusions are based on selected results and contradicting data have not been adequately taken into account. We point to other studies of higher quality performed on roseroot, several that found no significant effect and one that did. We conclude that the currently available evidence for the claimed effects is insufficient and that the effect of Rhodiola rosea is in need of further investigation before therapeutic claims can be made.
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| 14. |
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| 15. |
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| 16. |
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| 17. |
- Bucar, Franz, et al.
(författare)
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Amentoflavone from Biophytum sensitivum and its effect on COX-1/COX-2 catalysed prostaglandin biosynthesis
- 1998
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Ingår i: Planta Medica. - : Thieme. - 0032-0943 .- 1439-0221. ; 64:4, s. 373-374
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Tidskriftsartikel (refereegranskat)abstract
- Amentoflavone (13′, ll8-biapigenin) was isolated from the roots of Biophytum sensitivum DC. (Oxalidaceae) and proved to be a selective inhibitor of cyclooxygenase (COX)-1 catalysed prostaglandin biosynthesis when tested in vitro with an IC50 value of 12.4 µM (standard: indomethacin, IC50 = 1.1 µM). Doses of up to 37 µM showed only a slight inhibition in the corresponding COX-2 assay. Quantification of amentoflavone was carried out by reversed phase HPLC in methanolic and aqueous extracts of the roots, stems and leaves. Highest amounts of amentoflavone were detected in methanolic extracts of roots and stems (0.26-0.35%), while considerably lower amounts were detected in the corresponding water extracts.
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| 18. |
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| 19. |
- Cheng, Yajun, et al.
(författare)
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Curcumin decreases acid sphingomyelinase activity in colon cancer caco-2 cells
- 2007
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Ingår i: Planta Medica. - : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221. ; 73:8, s. 725-730
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Tidskriftsartikel (refereegranskat)abstract
- Curcumin has been shown to inhibit cell growth and induce apoptosis in colon cancer cells. The metabolism of sphingomyelin has implications in the development of colon cancer. We examined whether curcumin affects the enzymes that hydrolyse sphingomyelin in Caco-2 cells. The cells were cultured in both monolayer and polarized conditions and stimulated with curcumin. The activities of sphingomyelinases were determined. Sphingomyelin and its hydrolytic products were analysed by thin layer chromatography. The changes of acid sphingomyelinase protein were examined by Western blotting. We found that curcumin reduced the hydrolytic capacity of the cells against choline-labelled sphingomyelin, associated with a mild increase of cellular sphingomyelin in the cells. Analysis of the hydrolytic products revealed that the activity was derived from acid sphingomyelinase not from phospholipase D. The curcumin-induced reduction of acid SMase required more than 8 h stimulation. Western blotting showed reduced acid sphingomyelinase protein after curcumin stimulation. The inhibitory effect was more potent in monolayer cells than in polarised cells. No changes of other sphingomyelinases were identified. In the concentrations inhibiting acid sphingomyelinase, curcumin inhibited DNA synthesis and induced cell death. In conclusion, curcumin inhibits acid sphingomyelinase and the effect might be involved in its anti proliferative property against colon cancer cells.
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| 20. |
- Ekenäs, Catarina, et al.
(författare)
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Screening for anti-inflammatory activity of 12 arnica (asteraceae) species assessed by inhibition of NF-kappaB and release of human neutrophil elastase.
- 2008
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Ingår i: Planta Medica. - : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221. ; 74:15, s. 1789-1794
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Tidskriftsartikel (refereegranskat)abstract
- Several species in the genus Arnica have been used in traditional medicine to treat inflammatory-related disorders. Extracts of twelve Arnica species and two species closely related to arnica ( Layia hieracioides and Madia sativa) were investigated for inhibition of human neutrophil elastase release and inhibition of transcription factor NF-kappaB. Statistical analyses reveal significant differences in inhibitory capacities between extracts. Sesquiterpene lactones of the helenanolide type, of which some are known inhibitors of human neutrophil elastase release and NF-kappaB, are present in large amounts in the very active extracts of A. montana and A. chamissonis. Furthermore, A. longifolia, which has previously not been investigated, shows a high activity similar to that of A. montana and A. chamissonis in both bioassays. Sesquiterpene lactones of the xanthalongin type are present in large amounts in A. longifolia and other active extracts and would be interesting to evaluate further. COX-2:cyclooxygenase 2 EMSA:electrophoretic mobility shift assay fMLP: N-formyl-methionyl-leucyl-phenylalanine HaCaT:human keratinocyte HNE:human neutrophil elastase IkappaB:inhibitory subunit of kappaB iNOS:inducible nitric oxide synthase NF-kappaB:nuclear factor kappaB PAF:platelet activating factor STL:sesquiterpene lactone TNF-alpha:tumor necrosis factor alpha.
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| 21. |
- Ekenäs, Catarina, et al.
(författare)
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Screening for Anti-Inflammatory Activity of 12 Arnica (Asteraceae) Species Assessed by Inhibition of NF-κB and Release of Human Neutrophil Elastase
- 2008
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Ingår i: Planta Medica. - : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221. ; 74:15, s. 1789-1794
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Tidskriftsartikel (refereegranskat)abstract
- Several species in the genus Arnica have been used in traditional medicine to treat inflammatory-related disorders. Extracts of twelve Arnica species and two species closely related to Arnica (Layia hieracioides and Madia sativa) were investigated for inhibition of human neutrophil elastase release and inhibition of transcription factor NF-κB. Statistical analyses reveal significant differences in inhibitory capacities between extracts. Sesquiterpene lactones of the helenanolide type, of which some are known inhibitors of human neutrophil elastase release and NF-κB, are present in large amounts in the very active extracts of A. montana and A. chamissonis. Furthermore, A. longifolia, which has previously not been investigated, shows a high activity similar to that of A. montana and A. chamissonis in both bioassays. Sesquiterpene lactones of the xanthalongin type are present in large amounts in A. longifolia and other active extracts and would be interesting to evaluate further.
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| 22. |
- Endale, Milkyas, et al.
(författare)
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Antiplasmodial Quinones from Pentas longiflora and Pentas lanceolata
- 2012
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Ingår i: Planta Medica. - : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221. ; 78:1, s. 31-35
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Tidskriftsartikel (refereegranskat)abstract
- The dichloromethane/methanol (1 : 1) extracts of the roots of Pentas longiflora and Pentas lanceolata showed low micromolar (IC50 = 0.9-3 µg/mL) in vitro antiplasmodial activity against chloroquine-resistant (W2) and chloroquine-sensitive (D6) strains of Plasmodium falciparum. Chromatographic separation of the extract of Pentas longiflora led to the isolation of the pyranonaphthoquinones pentalongin (1) and psychorubrin (2) with IC50 values below 1 µg/mL and the naphthalene derivative mollugin (3), which showed marginal activity. Similar treatment of Pentas lanceolata led to the isolation of eight anthraquinones (4-11, IC50 = 5-31 µg/mL) of which one is new (5,6-dihydroxydamnacanthol, 11), while three - nordamnacanthal (7), lucidin-ω-methyl ether (9), and damnacanthol (10) - are reported here for the first time from the genus Pentas. The compounds were identified by NMR and mass spectroscopic techniques.
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| 23. |
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| 24. |
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| 25. |
- Fossen, Torgils, et al.
(författare)
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New Polyfunctional Phragmalin Limonoids from Neobeguea mahafalensis
- 2016
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Ingår i: Planta Medica. - : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221. ; 82:11-12, s. 1087-1095
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Tidskriftsartikel (refereegranskat)abstract
- Neobeguea mahafalensis is used as a medicinal plant inMadagascar. A decoction of the stem bark of this species is reported to treat back pain. Recently, it was reported that a decoction of the root bark, containing two novel phragmalin limonoids as identified active constituents, exhibited an extraordinarily high potency and remarkably long duration in augmenting sexual activity in male rodents. From the dichloromethane extract of the root barks of N. mahafalensis, nine phragmalin limonoids were isolated, of which eight were novel compounds. The structures were established mainly by extensive use of 2D NMR spectroscopic techniques and high-resolution mass spectrometry. One of the new compounds named dodoguin displayed sleep-inducing activity in Swiss albino mice. The amount of 3-10 mg/kg of this compound induced sleep 18-22 min after its administration with a duration of 16-18 min.
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| 26. |
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| 27. |
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| 28. |
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| 29. |
- Hansen, Kirstine M., et al.
(författare)
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Mo-clay for treatment of psoriasis
- 2016
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Ingår i: Planta Medica. - Stuttgart : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221.
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Konferensbidrag (refereegranskat)abstract
- Mo-clay was used by German doctors to treat injured soldiers' wounds during the First World War. Today, there are anecdotal cases of mo-clay being beneficial for patients suffering from psoriasis, a chronic, inflammatory disease. There are several histological features in the psoriatic skin, including acanthosis, hyperkeratosis, pararkeratosis and a loss of granular layer. Mo-clay is a unique marine deposit, an Eocene clayed diatomite. It was formed 54 million years ago from deposits of single-celled algae along with clay minerals and volcanic ash. The major elements are silicon, aluminium and iron. It is found in Denmark and Germany. As mo-clay had been used to treat wounds, it was tested for antibacterial activity. Mo-clay did not show any anti-bacterial activity against a battery of Gram-positive and -negative bacteria. Mo-clay showed stimulation of cell proliferation at concentrations 39 – 78 µg/ml in splenic mouse lymphocytes, and at 156 µg/ml in HaCat cells, whereas an inhibition of proliferation was observed at 313 µg/ml. Mo-clay was tested for anti-psoriatic activity in vivo using the mouse tail test [1]. This model can be used to investigate agents for effect on psoriasis, since the adult mouse tail has regions of both orthokeratosis and parakeratosis. Mo-clay induced orthokeratosis and showed a significant increase in epidermis thickness. The results suggest that mo-clay may have anti-psoriatic effects.
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| 30. |
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| 31. |
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| 32. |
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| 33. |
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| 34. |
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| 35. |
- Muceniece, Ruta, et al.
(författare)
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Potato (Solanum tuberosum) juice exerts an anticonvulsant effect in mice through binding to GABA receptors
- 2008
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Ingår i: Planta Medica. - : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221. ; 74:5, s. 491-496
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Tidskriftsartikel (refereegranskat)abstract
- Naturally occurring benzodiazepines have been identified in regular food such as wheat and potato, but there is still no evidence that potato extracts can affect CNS responses in vivo. Here we found that undiluted potato juice and potato juice diluted with saline 1:2 administered 10 min intracisternally (i.c.) and 30 min per os before bicuculline exerted significant anticonvulsant activity in the bicuculline-induced seizure threshold test in mice. In vitro, potato juice from different harvests at dilution series from 10% to 0.000001%, diluted 100,000-fold, displaced 50% of gamma-aminobutyric acid (GABA) receptor ligand [H-3]GABA and diluted 40-fold displaced 50% of [H-3]flunitrazepam from binding sites in mice forebrain membranes. The low content of diazepam (0.04 +/- 0.01 mg/kg) determined by HPLC and mass spectrometry in the potato extracts could not sustain the anticonvulsant activity of potato juice in vivo; therefore we hypothesized that potato juice might contain GABA(A) receptor GABA-site active compounds. The findings of this study suggest that potato juice as well as potato taken as food may have the capacity of influencing brain GABA-ergic activity.
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| 36. |
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| 37. |
- Noreen, Ylva, et al.
(författare)
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Flavan-3-ols isolated from some medicinal plants inhibiting COX-1 and COX-2 catalysed prostaglandin biosynthesis
- 1998
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Ingår i: Planta Medica. - : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221. ; 64:6, s. 520-524
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Tidskriftsartikel (refereegranskat)abstract
- Extracts from the four plant species Atuna racemosa Raf. ssp. racemosa, Syzygium corynocarpum (A. Gray) C. Muell., Syzygium malaccense (L.) Merr. & Perry and Vantanea peruviana Macbr., traditionally used for inflammatory conditions, were fractionated using a cyclooxygenase-1 catalysed prostaglandin biosynthesis in vitro assay. The flavan-3-ol derivatives (+)-catechin, (+)-gallocatechin, 4'-O-Me-ent-gallocatechin, ouratea-catechin and ouratea-proanthocynidin A were isolated as active principles. The IC50 values ranged from 3.3 mu M to 138 mu M whilst indomethacin under the same test conditions had an IC50 value of 1.1 mu M. The flavonol rhamnosides mearnsitrin, myricitrin and quercitrin were also isolated. When further tested for inhibitory effect on cyclooxygenase-2 catalysed prostaglandin biosynthesis, the five flavan-3-ol derivatives exhibited from equal to weaker inhibitory potencies, as compared to their cyclooxygenase-l inhibitory effects. The flavonol rhamnosides were inactive towards both enzymes.
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| 38. |
- Olsson, Erik M, et al.
(författare)
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A randomised, double-blind, placebo-controlled, parallel-group study of the standardised extract shr-5 of the roots of Rhodiola rosea in the treatment of subjects with stress-related fatigue.
- 2009
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Ingår i: Planta Medica. - : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221. ; 75:2, s. 105-12
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the study was to assess the efficacy of the standardised extract SHR-5 of roots of Rhodiola Rosea L. in the treatment of individuals suffering from stress-related fatigue. The phase III clinical trial took the form of a randomised, double-blind, placebo-controlled study with parallel groups. Participants, males and females aged between 20 and 55 years, were selected according to the Swedish National Board of Health and Welfare diagnostic criteria for fatigue syndrome. A total of 60 individuals were randomised into two groups, one ( N = 30) of which received four tablets daily of SHR-5 extract (576 mg extract/day), while a second ( N = 30) received four placebo tablets daily. The effects of the extract with respect to quality of life (SF-36 questionnaire), symptoms of fatigue (Pines' burnout scale), depression (Montgomery -Asberg depression rating scale - MADRS), attention (Conners' computerised continuous performance test II - CCPT II), and saliva cortisol response to awakening were assessed on day 1 and after 28 days of medication. Data were analysed by between-within analyses of variance. No serious side effects that could be attributed to the extract were reported. Significant post-treatment improvements were observed for both groups (placebo effect) in Pines' burnout scale, mental health (SF-36), and MADRS and in several CCPT II indices of attention, namely, omissions, commissions, and Hit RT SE. When the two groups were compared, however, significant effects of the SHR-5 extract in comparison with the placebo were observed in Pines' burnout scale and the CCPT II indices omissions, Hit RT SE, and variability. Pre- VERSUS post-treatment cortisol responses to awakening stress were significantly different in the treatment group compared with the control group. It is concluded that repeated administration of R. ROSEA extract SHR-5 exerts an anti-fatigue effect that increases mental performance, particularly the ability to concentrate, and decreases cortisol response to awakening stress in burnout patients with fatigue syndrome.
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| 39. |
- Papaspyridi, Lefki Maria, et al.
(författare)
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Optimization of biomass production with enhanced bioactive compound content by the medicinal mushroom Ganoderma australe under submerged culture
- 2011
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Ingår i: Planta Medica. - : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221. ; 77:12, s. 1282-1282
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Tidskriftsartikel (refereegranskat)abstract
- Mushrooms have become attractive as functional foods while they represent an untapped source for the development of drugs and nutraceuticals. Comparing with the established field cultivation, their submerged cultivation has significant industrial potential for the effective production of biomass and valuable metabolites [1]. In this study, Ganoderma australe (Fr.) Pat. (strain ATHUM 4345, collected from Greece), which is a species of pharmaceutical interest [2] was investigated for maximum biomass production with enhanced dietary fiber and bioactive β-glucan content in a batch-stirred tank bioreactor. Specifically, when the optimized culture medium was tested in a 20-L stirred tank bioreactor, high biomass yields (10.1±0.4g/L) and productivity of 0.09g L-1h-1 were obtained. The yield coefficients for total glucan and dietary fibers on biomass formed were 94.9±6 and 341.2±12.3mg/g mycelium dry weight, respectively [3]. Additionally, the biomass produced by the process described above, was lyophilized and finally extracted with ethyl acetate:cyclohexane. Subsequently, the fractionation of the resulting extract by chromatographic techniques led to the isolation and identification of linoleic acid, 19-octacosenoic acid, 9-palmitoleic acid, ergosta-5,7,22-trien-3β-ol and a derivative of the triterpene austrolactone. All compounds isolated, were identified by means of spectral data (1H-NMR and 2D NMR), HRMS and direct comparison with the respective literature data. The findings of this study are valuable, as the established fermentation process, led to the efficient production of biomass containing bioactive compounds. Those results demonstrate the potential of producing high added value bioactive compounds from G. australe strain under submerged culture conditions on an industrial scale.
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| 40. |
- Pohjala, L., et al.
(författare)
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The isoflavone biochanin a inhibits the growth of the intracellular bacteria Chiamydia trachomatis and Chlamydia pneumoniae
- 2012
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Ingår i: Planta Medica. - : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221. ; 78:11, s. 1102-1102
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Epidemiology and physiological consequences of chlamydial infections show these intracellular bacteria to have maintained their prevalence. Especially C. pneumoniae are able to confer to a treatment refractory chronic state of infection that cannot be eradicated with currently available therapeutic options. Here we report the effects of biochanin A on the growth of intracellular Chlamydia spp. It is the main flavonoid component of red clover (Trifolium pratense) extracts, which besides its estrogenic and antioxidative properties is known to potentiate the antibacterial effects of other chemical agents by inhibiting bacterial efflux pumps. We identified biochanin A as a hit compound in a high-content screen of purified natural products for C. trachomatis growth inhibitors. It was found to inhibit the replication of C. pneumoniae clinical strain K7 (IC =12μM) and to prevent 100% of infectious progeny production at 50μM. Thus, biochanin A is a more potent inhibitor of C. pneumoniae than the related isoflavone genistein, which we have earlier shown to be only moderately active against this bacterium. Further, this data suggests that biochanin A acts as a direct growth inhibitor rather than an antibacterial potentiator against these pathogens.
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| 41. |
- Razafimahefa, Solofoniaina, et al.
(författare)
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Libiguins A and B : Novel Phragmalin Limonoids Isolated from Neobeguea mahafalensis Causing Profound Enhancement of Sexual Activity
- 2014
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Ingår i: Planta Medica. - : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221. ; 80:4, s. 306-314
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Tidskriftsartikel (refereegranskat)abstract
- In a screening programme directed towards the discovery of drugs that could enhance sexual activity, we found that a decoction of the root bark of Neobeguea mahafalensis displayed an extraordinarily high potency and remarkably long duration in augmenting sexual activity in male rodents. Bioassay-guided fractionation led to the isolation of two pharmacoactive constituents, which turned out to be novel 1,8,9-orthoacetate phragmalin limonoids that we named libiguins A and B, each with a C-16/30 -lactone ring. Chemical structures were established by the interpretation of their 1D and 2DNMR data. In vivo pharmacological tests showed that starting with a treatment from 0.004-0.4mg/kg/day for three consecutive days, over a 3-h sampling period, these limonoids induced a long-lasting augmentation of frequency and sustainment of mounting behaviour in male rodents, with an effect lasting for up to 11 days post-treatment. Libiguin A proved to be markedly more potent than libiguin B. This report is the first of limonoids having such an effect, and the findings could lead to novel therapies for the treatment of sexual dysfunction. Moreover, the results can serve as an opening to elucidate the central physiological control of mating behaviour, which is still not well mapped out.
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| 42. |
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| 43. |
- Segura, Laura, et al.
(författare)
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Anti-inflammatory activity of dichloromethane extract of Heterotheca inuloides in vivo and in vitro
- 2000
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Ingår i: Planta Medica. - : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221. ; 66:6, s. 553-555
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Tidskriftsartikel (refereegranskat)abstract
- The dichloromethane extract from the dried flowers of Heterotheca inuloides Cass. was investigated on several pharmacological models of inflammation in vivo and in vitro. It showed anti-inflammatory activity on the croton oil-induced oedema test in mouse ear, at 1 mg/ear. The compound isolated from this extract, 7-hydroxy-3,4-dihydrocadalin, showed anti-inflammatory effect on the same experimental model (ED50 of 0.9 mumol/ear), as well as on COX-1 and COX-2 catalysed prostaglandin biosynthesis assays, with IC50 values of 22 microM and 526 microM, respectively. No effect was observed on carrageenan-induced oedema and on fMLP/PAF-induced exocytosis of human neutrophils. The COX-1 inhibitory effect showed by 7-hydroxy-3,4-dihydrocadalin might be related to the anti-inflammatory activity on the topical oedema induced by croton oil.
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| 44. |
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| 45. |
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| 46. |
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| 47. |
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| 48. |
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| 49. |
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| 50. |
- Vasänge, Mervi, et al.
(författare)
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The flavonoid constituents of two Polypodium species (Calaguala) and their effect on the elastase release in human neutrophils
- 1997
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Ingår i: Planta Medica. - : Georg Thieme Verlag KG. - 0032-0943 .- 1439-0221. ; 63:6, s. 511-517
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Tidskriftsartikel (refereegranskat)abstract
- Five flavonoid compounds were isolated from two Polypodium species (P.decumanum and P.triseriale) with the common name Calaguala. Structure elucidation was carried out using different NMR techniques and revealed the presence of one new glycoside (kaempferol 3-O-beta-D-xylopyranosyl-(1-2)-beta-D-arabinopyranoside) (1), two known flavonoid glycosides, rutin and kaempferol 3-O-alpha-D-arabinopyranoside (2,3), the trimeric proanthocyanidin, selligueain (4), and the coumarinic acid derivative, melilotoside (5). The compounds were tested for their activity in PAF induced exocytosis in human neutrophils but none of the compounds showed PAF specific activity. Instead, they showed more general effects on the neutrophil including inhibition of the spontaneous elastase release (5) and potentiation of the release induced by PAF (1). Selligueain was found to inhibit the proteolytic enzyme, elastase in vitro.
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