| 1. |
- Stollenwerk, Maria, et al.
(författare)
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Quantitation of bacterial adhesion to polymer surfaces by bioluminescence
- 1998
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Ingår i: Zentralblatt fur Bakteriologie. - 0934-8840. ; 287:1-2, s. 7-18
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Tidskriftsartikel (refereegranskat)abstract
- Quantitation of microbes adhering to a surface is commonly used in studies of microbial adhesion to different surfaces. We have quantified different staphylococcal strains adhering to polymer surfaces by measuring bacterial ATP (adenosine triphosphate) by bioluminescence. The method is sensitive, having a detection limit of 104 bacterial cells. Viable counting of bacterial cells may yield falsely low results due to the presence of 'dormant' and adherent bacteria. By using bioluminescence, this can be avoided. Cells of different bacterial species and cells of strains of the same species were shown to differ significantly in their basal ATP content (8.7 x 10-13 - 5.2 x 10-22 MATP). The size of adherent and planktonic bacteria decreased with time (0.7 μm → 0.3 μm, 20 days). During incubation in nutrient-poor buffer ('starvation'), the ATP content of adherent bacteria decreased after 24-96 h whereas that of planktonic bacteria was stable over 20 days. The presence of human serum or plasma did not interfere significantly with the test results. Since the ATP concentration of bacterial strains of different species varies and is also influenced by the growth conditions of bacteria (solid or liquid culture medium), a species-specific standard curve has to be established for bacteria grown under the same culture conditions. We conclude that the method is a sensitive tool to quantify adherent bacteria during experiments lasting for less than 6 h and constitutes a valuable method to be used in conjunction with different microscopical techniques.
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| 2. |
- Svanborg, Catharina, et al.
(författare)
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Bacterial Adherence and Epithelial Cell Cytokine Production
- 1993
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Ingår i: Zentralblatt fur Bakteriologie. - 0934-8840. ; 278:2-3, s. 359-364
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Tidskriftsartikel (refereegranskat)abstract
- Epithelial cell lines produce the cytokines IL-1α, IL-6 and IL-8 when stimulated with Escherichia coli. The cytokine response is enhanced by P fimbriae. The epithelial cell lines also respond to stimulation with other cytokines. These in vitro findings were confirmed in vivo in patients with E. coli infection who secreted IL-6 and IL-8 into the urine. The observations suggest that epithelial cells play a more active role in the mucosal immune response than previously recognized.
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| 3. |
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| 4. |
- Cimmino, M, et al.
(författare)
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European Lyme borreliosis clinical spectrum
- 1998
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Ingår i: Zentralblatt fur Bakteriologie : international journal of medical microbiology. - : Elsevier BV. - 0934-8840. ; 287:3, s. 248-252
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Tidskriftsartikel (refereegranskat)
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| 5. |
- Gray, JS, et al.
(författare)
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Lyme borreliosis awareness
- 1998
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Ingår i: Zentralblatt fur Bakteriologie : international journal of medical microbiology. - : Elsevier BV. - 0934-8840. ; 287:3, s. 253-265
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Tidskriftsartikel (refereegranskat)
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| 6. |
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| 7. |
- O'Connell, S, et al.
(författare)
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Epidemiology of European Lyme borreliosis
- 1998
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Ingår i: Zentralblatt fur Bakteriologie : international journal of medical microbiology. - : Elsevier BV. - 0934-8840. ; 287:3, s. 229-240
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Tidskriftsartikel (refereegranskat)
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| 8. |
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| 9. |
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| 10. |
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| 11. |
- Wang, Xin, et al.
(författare)
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Dietary factors influence the recovery rates of Helicobacter pylori in a BALB/cA mouse model
- 1998
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Ingår i: Zentralblatt für Bakteriologie: Ternational Journal of Medical Microbiology: Medical Microbiology, Virology, Parasitology, Infectious Diseases. - 0934-8840. ; 288:2, s. 195-205
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to assess the ability of different mouse diets to sustain an H. pylori infection in BALB/cA mice. Four commercially available mouse diets were compared. Experiment 1: Mice were fed the four diets for seven days before infection, infected three times at two-day intervals with 0.1 ml of 10(9) colony-forming units/ml H. pylori cells. H. pylori strains (n = 4) were cultured on GAB-Camp agar for 2 days, harvested and suspended in PBS. All animals were sacrificed at 2 and 4 weeks post inoculation. Experiment 2: Mice infected for 8 weeks were fed RM2, changed to the different diets for 10 days and sacrificed. Stomachs were collected, cultured on GAB-Camp agar to estimate H. pylori growth and stomach biopsies were analyzed by PCR. There were significant differences between diets in their ability to sustain growth of H. pylori. The range was from a few hundred colonies to no growth at all on the GAB-Camp agar. PCR signals showed good correlation with the culture results. All H. pylori-infected mice gave a significantly higher inflammation score compared to non-infected mice. The diet RM2, having the highest number of culturable H. pylori in the mouse stomach, also showed the highest inflammation. These results suggest that the dietary factors affect the amounts of H. pylori in an infection of BALB/cA mice.
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