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Sökning: L773:0940 6360 OR L773:1432 1890

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1.
  • Andresen, Louise C., 1974, et al. (författare)
  • Patterns of free amino acids in tundra soils reflect mycorrhizal type, shrubification, and warming
  • 2022
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 32:3-4, s. 305-313
  • Tidskriftsartikel (refereegranskat)abstract
    • The soil nitrogen (N) cycle in cold terrestrial ecosystems is slow and organically bound N is an important source of N for plants in these ecosystems. Many plant species can take up free amino acids from these infertile soils, either directly or indirectly via their mycorrhizal fungi. We hypothesized that plant community changes and local plant community differences will alter the soil free amino acid pool and composition; and that long-term warming could enhance this effect. To test this, we studied the composition of extractable free amino acids at five separate heath, meadow, and bog locations in subarctic and alpine Scandinavia, with long-term (13 to 24 years) warming manipulations. The plant communities all included a mixture of ecto-, ericoid-, and arbuscular mycorrhizal plant species. Vegetation dominated by grasses and forbs with arbuscular and non-mycorrhizal associations showed highest soil free amino acid content, distinguishing them from the sites dominated by shrubs with ecto- and ericoid-mycorrhizal associations. Warming increased shrub and decreased moss cover at two sites, and by using redundancy analysis, we found that altered soil free amino acid composition was related to this plant cover change. From this, we conclude that the mycorrhizal type is important in controlling soil N cycling and that expansion of shrubs with ectomycorrhiza (and to some extent ericoid mycorrhiza) can help retain N within the ecosystems by tightening the N cycle.
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2.
  • Berner, Christoffer, et al. (författare)
  • Long-term effect of apatite on ectomycorrhizal growth and community structure.
  • 2012
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 1432-1890 .- 0940-6360. ; 22:8, s. 615-621
  • Tidskriftsartikel (refereegranskat)abstract
    • Ectomycorrhizal (ECM) fungi are efficient at taking up phosphorus (P) from mineral sources, such as apatite, which are not easily available to the host trees. Since ECM fungal species differ in P uptake rates, it can be expected that the composition of the ECM fungal community will change upon exposure to apatite, provided that the P transfer is rewarded by more carbon being transferred to the fungal symbiont. Control and apatite-amended mesh bags were buried in pairs in the humus layer of a P-poor Norway spruce forest. The ECM fungal community that colonized these bags was analyzed by DNA extraction, PCR amplification of the internal transcribed spacer (ITS) region, cloning, and random sequencing. Fungal biomass was estimated by ergosterol analysis. No change in the ECM fungal community structure was seen after 5 years of apatite exposure, although the fungal biomass increased threefold upon apatite amendment. Our results indicate that host trees enhance carbon allocation to ECM fungi colonizing P sources in P-poor forests but the lack of change in the composition of the ECM fungal community suggests that P transfer rates were similar among the species. Alternatively, higher P transfer among certain species was not rewarded with higher carbon transfer from the host.
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3.
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4.
  • Castaño Soler, Carles (författare)
  • White mulch and irrigation increase black truffle soil mycelium when competing with summer truffle in young truffle orchards
  • 2021
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 31, s. 371-382
  • Tidskriftsartikel (refereegranskat)abstract
    • The black truffle (Tuber melanosporum Vittad.) and the summer truffle (Tuber aestivum Vittad.) are two of the most appreciated edible fungi worldwide. The natural distributions of both species partially overlap. However, the interspecific interactions between these truffles and how irrigation and mulching techniques impact the dynamics between them are still unknown. Here, an experimental truffle plantation with Quercus ilex was established in Maials (Catalonia, Spain), combining three soil mulch treatments (white mulch, black mulch and bare soil as a control) and two irrigation regimes (irrigated and non-irrigated as a control) to investigate truffle mycelial dynamics in soil when both truffle species co-occur. The development of truffle mycelium in two different seasons (spring and autumn) in two consecutive years (2017 and 2018) was quantified using qPCR. Truffle mycelia of both species showed greatest development under white mulch. When mycelia of both truffle species co-occurred in soil, irrigation combined with white mulch resulted in greater quantities of T. melanosporum mycelial biomass, whereas the control irrigation treatment favoured the development of T. aestivum. Mulch treatments were also advantageous for seedling growth, which was expressed as root collar diameter and its increment during the study period. Significant relationships between root collar diameter and root growth and the amount of mycelial biomass in the soil were observed for both truffle species. Our results indicate the potential advantages of using white mulch to support irrigation in truffle plantations located in areas with dry Mediterranean climatic conditions to promote the development of Tuber mycelium.
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5.
  • Dunham, S. M., et al. (författare)
  • Species richness and community composition of mat-forming ectomycorrhizal fungi in old- and second-growth Douglas-fir forests of the HJ Andrews Experimental Forest, Oregon, USA
  • 2007
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 17:8, s. 633-645
  • Tidskriftsartikel (refereegranskat)abstract
    • We investigated the species identity of mat-forming ectomycorrhizal (EM) fungi associated with old- and second-growth Douglas-fir stands. Using molecular analyses of rhizomorphs and EM root tips, we characterized 28 unique internal transcribed spacer sequences and considered them proxies for mat-forming EM species. In both stand age classes, one Athelioid species in the genus Piloderma dominated our sample of the mat-forming fungal community. In second-growth stands, the second most frequently encountered mat-forming EM species belonged to the genus Hysterangium. In old-growth stands, several Ramaria species were associated with a frequently encountered mat morphology but no species dominated the community. After using rarefaction analysis to standardize sampling effort, the total species richness did not differ statistically between old- and second-growth habitats. Both an abundance of infrequently encountered species and incomplete sampling of the mat-forming EM community may have limited our ability to detect potential differences in species richness. Several frequently encountered Piloderma species appear to have broad (holarctic) distributions and diverse host associations and their potential importance in forest ecosystems warrants further study.
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6.
  • Ek, Hans, et al. (författare)
  • Carbon and nitrogen flow in Silver birch and Norway spruce connected by a common mycorrhizal mycelium
  • 1996
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 1432-1890 .- 0940-6360. ; 6:6, s. 465-467
  • Tidskriftsartikel (refereegranskat)abstract
    • Spruce and birch seedlings were grown together in boxes filled with unsterile pear. Both seedlings were colonized by the ectomycorrhizal fungus Scleroderma citrinum. The two plants thus shared a common external mycelium. N-15-labelled ammonium was supplied exclusively to the fungus, while the birch or the spruce plant was continuously fed with C-13-labelled CO2 for 72 h. The carbon and nitrogen transfer rates were strikingly different for birch and spruce seedlings. The mycorrhizal mycelium received carbohydrates mainly from the birch plant and the nitrogen transfer by the fungus to the plants was largely directed towards the birch. Carbon assimilates were also transferred in both directions between birch and spruce; however, there was no conclusive evidence for a net transfer of carbon between the plants.
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7.
  • Eshghi Sahraei, Shadi, et al. (författare)
  • Whole genome analyses based on single, field collected spores of the arbuscular mycorrhizal fungus Funneliformis geosporum
  • 2022
  • Ingår i: Mycorrhiza. - : Springer. - 0940-6360 .- 1432-1890. ; 32:5-6, s. 361-371
  • Tidskriftsartikel (refereegranskat)abstract
    • Arbuscular mycorrhizal (AM) fungi are ubiquitous mutualistic symbionts of most terrestrial plants and many complete their lifecycles underground. Whole genome analysis of AM fungi has long been restricted to species and strains that can be maintained under controlled conditions that facilitate collection of biological samples. There is some evidence suggesting that AM fungi can adapt to culture resulting in phenotypic and possibly also genotypic changes in the fungi. In this study, we used field isolated spores of AM fungi and identified them as Funneliformis geosporum based on morphology and phylogenetic analyses. We separately assembled the genomes of two representative spores using DNA sequences of 19 and 22 individually amplified nuclei. The genomes were compared with previously published data from other members of Glomeraceae including two strains of F. mosseae. No significant differences were observed among the species in terms of gene content, while the single nucleotide polymorphism density was higher in the strains of F. geosporum than in the strains of F. mosseae. In this study, we demonstrate that it is possible to sequence and assemble genomes from AM fungal spores sampled in the field, which opens up the possibility to include uncultured AM fungi in phylogenomic and comparative genomic analysis and to study genomic variation in natural populations of these important plant symbionts.
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8.
  • Fransson, Petra (författare)
  • Carbon availability affects already large species-specific differences in chemical composition of ectomycorrhizal fungal mycelia in pure culture
  • 2023
  • Ingår i: Mycorrhiza. - 0940-6360 .- 1432-1890. ; 33, s. 303-319
  • Tidskriftsartikel (refereegranskat)abstract
    • Although ectomycorrhizal (ECM) contribution to soil organic matter processes receives increased attention, little is known about fundamental differences in chemical composition among species, and how that may be affected by carbon (C) availability. Here, we study how 16 species (incl. 19 isolates) grown in pure culture at three different C:N ratios (10:1, 20:1, and 40:1) vary in chemical structure, using Fourier transform infrared (FTIR) spectroscopy. We hypothesized that C availability impacts directly on chemical composition, expecting increased C availability to lead to more carbohydrates and less proteins in the mycelia. There were strong and significant effects of ECM species (R-2 = 0.873 and P = 0.001) and large species-specific differences in chemical composition. Chemical composition also changed significantly with C availability, and increased C led to more polysaccharides and less proteins for many species, but not all. Understanding how chemical composition change with altered C availability is a first step towards understanding their role in organic matter accumulation and decomposition.
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9.
  • Hagerberg, David, et al. (författare)
  • The elemental content in the mycelium of the ectomycorrhizal fungus Piloderma sp during the colonization of hardened wood ash
  • 2005
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 1432-1890 .- 0940-6360. ; 15:6, s. 387-392
  • Tidskriftsartikel (refereegranskat)abstract
    • Piloderma sp., a wood ash-colonizing ectomycorrhizal (EM) fungus, was grown symbiotically with Norway spruce in microcosms which contained granules of hardened wood ash. Mycelium close to the granules was sampled 3 times over a period of 11 weeks and the elemental content was investigated with particle induced X-ray emission. Mycelium from microcosms without wood ash was used as controls. The contents of P and K were similar in mycelium growing close to wood ash granules to those in control mycelium, while the Ca content increased from 23 +/- 21 mg g(-1) in controls to 63 +/- 8 mg g(-1) in mycelium growing close to wood ash granules. The Ca content was also increased in other parts of the mycelium more distant from the wood ash. Piloderma sp. may have a role in the short-term storage of Ca released from wood ash, rather than in releasing and storing P.
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10.
  • Hammer, Edith, et al. (författare)
  • Elemental composition of arbuscular mycorrhizal fungi at high salinity.
  • 2011
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 1432-1890 .- 0940-6360. ; 21, s. 117-129
  • Tidskriftsartikel (refereegranskat)abstract
    • We investigated the elemental composition of spores and hyphae of arbuscular mycorrhizal fungi (AMF) collected from two saline sites at the desert border in Tunisia, and of Glomus intraradices grown in vitro with or without addition of NaCl to the medium, by proton-induced X-ray emission. We compared the elemental composition of the field AMF to those of the soil and the associated plants. The spores and hyphae from the saline soils showed strongly elevated levels of Ca, Cl, Mg, Fe, Si, and K compared to their growth environment. In contrast, the spores of both the field-derived AMF and the in vitro grown G. intraradices contained lower or not elevated Na levels compared to their growth environment. This resulted in higher K:Na and Ca:Na ratios in spores than in soil, but lower than in the associated plants for the field AMF. The K:Na and Ca:Na ratios of G. intraradices grown in monoxenic cultures were also in the same range as those of the field AMF and did not change even when those ratios in the growth medium were lowered several orders of magnitude by adding NaCl. These results indicate that AMF can selectively take up elements such as K and Ca, which act as osmotic equivalents while they avoid uptake of toxic Na. This could make them important in the alleviation of salinity stress in their plant hosts.
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11.
  • Hasselquist, Niles (författare)
  • First report of the ectomycorrhizal status of boletes on the Northern Yucatan Peninsula, Mexico determined using isotopic methods
  • 2011
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 21, s. 465-471
  • Tidskriftsartikel (refereegranskat)abstract
    • Despite their prominent role for tree growth, few studies have examined the occurrence of ectomycorrhizal fungi in lowland, seasonally dry tropical forests (SDTF). Although fruiting bodies of boletes have been observed in a dry tropical forest on the Northern Yucatan Peninsula, Mexico, their occurrence is rare and their mycorrhizal status is uncertain. To determine the trophic status (mycorrhizal vs. saprotrophic) of these boletes, fruiting bodies were collected and isotopically compared to known saprotrophic fungi, foliage, and soil from the same site. Mean delta N-15 and delta C-13 values differed significantly between boletes and saprotrophic fungi, with boletes 8.0% enriched and 2.5% depleted in N-15 and C-13, respectively relative to saprotrophic fungi. Foliage was depleted in C-13 relative to both boletes and saprotrophic fungi. Foliar delta N-15 values, on the other hand, were similar to saprotrophic fungi, yet were considerably lower relative to bolete fruiting bodies. Results from this study provide the first isotopic evidence of ectomycorrhizal fungi in lowland SDTF and emphasize the need for further research to better understand the diversity and ecological importance of ectomycorrhizal fungi in these forested ecosystems.
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12.
  • Hedh, Jenny, et al. (författare)
  • Variation in host specificity and gene content in strains from genetically isolated lineages of the ectomycorrhizal fungus Paxillus involutus s. lat.
  • 2009
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 1432-1890 .- 0940-6360. ; 19, s. 549-558
  • Tidskriftsartikel (refereegranskat)abstract
    • Ectomycorrhizal fungi are known to vary in host range. Some fungi can enter into symbiosis with multiple plant species, while others have restricted host ranges. The aim of this study was to examine variation in host specificity among strains from the basidiomycete Paxillus involutus s. lat. Recent studies have shown that this fungus consists of at least four genetically isolated lineages, phylogenetic species (PS) I (which corresponds to the morphological species Paxillus obscurosporus), PS II (P. involutus s. str.), PS III (Paxillus validus), and PS IV (not yet supported by any reference material). Thirty-five Paxillus strains of PS I to IV were examined in microcosms for their capacity to infect birch (Betula pendula) and spruce (Picea abies). Seventeen strains were compatible and formed mycorrhizae with both tree species. Seven strains were incompatible with both birch and spruce. The gene content in three pairs of incompatible and compatible strains PS I, II, and III were compared using microarray-based comparative genomic hybridizations. Of 4,113 P. involutus gene representatives analyzed, 390 varied in copy numbers in at least one of the three pairwise comparisons. Only three reporters showed significant changes in all three pairwise comparisons, and none of these were changed in a similar way in three comparisons. Our data indicate that changes in host range have occurred frequently and independently among strains in P. obscurosporus, P. involutus s. str., and P. validus. No evidence was obtained demonstrating that these changes have been associated with the gain or loss of similar genes in these three species.
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13.
  • Heidarianpour, Mohammad Bagher, et al. (författare)
  • Positive effects of co-inoculation with Rhizophagus irregularis and Serendipita indica on tomato growth under saline conditions, and their individual colonization estimated by signature lipids
  • 2020
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 30:4, s. 455-466
  • Tidskriftsartikel (refereegranskat)abstract
    • Tomato roots can be colonized by both mycorrhizal fungi and the endophytic fungus Serendipita indica. This study was aimed at assessment of the impact of single or dual inoculation with R. irregularis and S. indica on tomato growth under saline conditions. We used signature compounds to estimate the abundance of each of these two fungi (fatty acid 16:1ω5 for R. irregularis and ergosterol for S. indica) in roots. A randomized block design was applied with four types of inoculation (no fungus, R. irregularis, S. indica or S. indica + R. irregularis) at different levels of salinity (1.2, 5, and 10 dS/m) with four replications per treatment. The plant dry weight was slightly higher in R. irregularis– than S. indica–inoculated plants, and the highest plant biomass was achieved with dual inoculation. R. irregularis increased the content of the neutral lipid fatty acid 16:1ω5 from 97 to 5300 nmol/g and phospholipid fatty acid 16:1ω5 from 8 to 141 nmol/g in roots (at a salinity level of 1.2 dS m−1), but the increases were lower at higher levels of salinity. Moreover, both these arbuscular mycorrhizal fungal markers were slightly decreased in the presence of S. indica. Root ergosterol increased from 7 to 114 μg g−1 with S. indica inoculation. With increasing salinity, the concentration of ergosterol in roots decreased. Inoculation with R. irregularis caused a decrease in root ergosterol. Increasing salinity resulted in an increase of Cl and Na in tomato shoots, but the increase was significantly lower in single- or dual-inoculated plants in contrast to the control plants.
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14.
  • Heller, Gregory, et al. (författare)
  • Expression analysis of Clavata1-like and Nodulin21-like genes from Pinus sylvestris during ectomycorrhiza formation
  • 2012
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 22, s. 271-277
  • Tidskriftsartikel (refereegranskat)abstract
    • The ecology and physiology of ectomycorrhizal (EcM) symbiosis with conifer trees are well documented. In comparison, however, very little is known about the molecular regulation of these associations. In an earlier study, we identified three EcM-regulated Pinus expressed sequence tags (EST), two of which were identified as homologous to the Medicago truncatula nodulin MtN21. The third EST was a homologue to the receptor-like kinase Clavata1. We have characterized the expression patterns of these genes and of auxin- and mycorrhiza-regulated genes after induction with indole-3-butyric acid in Pinus sylvestris and in a time course experiment during ectomycorrhizal initiation with the co-inoculation of 2,3,5-triiodobenzoic acid, an auxin transport inhibitor. Our results suggest that different P. sylvestris nodulin homologues are associated with diverse processes in the root. The results also suggest a potential role of the Clv1-like gene in lateral root initiation by the ectomycorrhizal fungus.
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15.
  • Hupperts, Stefan (författare)
  • Predictors of taxonomic and functional composition of black spruce seedling ectomycorrhizal fungal communities along peatland drainage gradients
  • 2022
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 32, s. 67-81
  • Tidskriftsartikel (refereegranskat)abstract
    • Many trees depend on symbiotic ectomycorrhizal fungi for nutrients in exchange for photosynthetically derived carbohydrates. Trees growing in peatlands, which cover 3% of the earth's terrestrial surface area yet hold approximately one-third of organic soil carbon stocks, may benefit from ectomycorrhizal fungi that can efficiently forage for nutrients and degrade organic matter using oxidative enzymes such as class II peroxidases. However, such traits may place a higher carbon cost on both the fungi and host tree. To investigate these trade-offs that might structure peatland ectomycorrhizal fungal communities, we sampled black spruce (Picea mariana (Mill.)) seedlings along 100-year-old peatland drainage gradients in Minnesota, USA, that had resulted in higher soil nitrogen and canopy density. Structural equation models revealed that the relative abundance of the dominant ectomycorrhizal fungal genus, Cortinarius, which is known for relatively high fungal biomass coupled with elevated class II peroxidase potential, was negatively linked to site fertility but more positively affected by recent host stem radial growth, suggesting carbon limitation. In contrast, Cenococcum, known for comparatively lower fungal biomass and less class II peroxidase potential, was negatively linked to host stem radial growth and unrelated to site fertility. Like Cortinarius, the estimated relative abundance of class II peroxidase genes in the ectomycorrhizal community was more related to host stem radial growth than site fertility. Our findings indicate a trade-off between symbiont foraging traits and associated carbon costs that consequently structure seedling ectomycorrhizal fungal communities in peatlands.
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16.
  • Ishida, Takahide (författare)
  • Ectomycorrhizal fungal community in alkaline-saline soil in northeastern China
  • 2009
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 19, s. 329-335
  • Tidskriftsartikel (refereegranskat)abstract
    • Alkaline-saline soil is widespread in arid and semiarid regions of the world and causes severe environmental and agricultural problems. To advance our understanding of the adaptation of ectomycorrhizal fungi (EMF) to alkaline-saline soil, we investigated EMF communities on Mongolian willow (Salix linearistipularis) growing in alkaline-saline soil (up to pH 9.2) in northeastern China. In total, 75 root samples were collected from 25 willow individuals over 4.7 ha. To identify fungal species in ectomycorrhizal root tips, we used terminal restriction fragment length polymorphism and sequencing analyses of the internal transcribed spacer region of ribosomal DNA. We detected 11 EMF species, including species of Inocybe, Hebeloma, and Tomentella of the Basidiomycota and three Ascomycota species. The EMF richness of the study site was estimated to be 15-17 using major estimators. The most abundant species was Geopora sp. 1, while no Geopora-dominated EMF communities have been reported so far. Phylogenetic analysis showed that the phylogroup including Geopora sp. 1 has been found mostly in alkaline soil habitats, indicating its adaptation to high soil pH. Because EMF are indispensable for host plant growth, the EMF species detected in this study may be useful for restoration of alkaline-saline areas.
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17.
  • Izumi, Hironari, et al. (författare)
  • Suillus mycelia under elevated atmospheric CO2 support increased bacterial communities and scarce nifH gene activity in contrast to Hebeloma mycelia
  • 2013
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 23, s. 155-165
  • Tidskriftsartikel (refereegranskat)abstract
    • Bacterial communities associated with mycorrhizal roots are likely to respond to rising atmospheric CO2 levels in terms of biomass, community composition and activity since they are supported by the carbon (C) flow outside the root tips, especially by exudation of low molecular weight organic compounds. We studied how general bacterial and diazotrophic communities associated with ectomycorrhizal (ECM) fungi respond to different belowground C supply conditions, mediated by elevated atmospheric CO2 concentration under nitrogen (N) limited conditions. Microcosm systems were constructed using forest soil and Scots pine seedlings, which were either pre-inoculated with one of the ECM fungal species Hebeloma velutipes or Suillus variegatus, or non-inoculated. These fungal species differ in C allocation and exudation patterns. Seedlings were maintained under ambient (380 ppm) or elevated (700 ppm) CO2 levels for 6 months. Quantitative polymerase chain reaction (PCR) showed a significant increase in 16S rRNA gene copy numbers for Suillus-inoculated microcosms under elevated CO2 compared to ambient CO2. The copy numbers of the nitrogenase reductase (nifH) gene were under the detection limit in all samples regardless the CO2 treatments. Denaturing gradient gel electrophoresis analysis of PCR-amplified nifH genes revealed simple and consistent communities in all samples throughout the incubation period. A nested reverse transcription PCR approach revealed that expression of nifH genes were detected in some microcosms. Our findings suggest that the effect of mycorrhizal fungi on soil bacteria may vary depending on C supply and fungal species.
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18.
  • Kohout, Petr, et al. (författare)
  • Ericaceous dwarf shrubs affect ectomycorrhizal fungal community of the invasive Pinus strobus and native Pinus sylvestris in a pot experiment.
  • 2011
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 21:5, s. 403-12
  • Tidskriftsartikel (refereegranskat)abstract
    • This study aimed to elucidate the relationship between ericaceous understorey shrubs and the diversity and abundance of ectomycorrhizal fungi (EcMF) associated with the invasive Pinus strobus and native Pinus sylvestris. Seedlings of both pines were grown in mesocosms and subjected to three treatments simulating different forest microhabitats: (a) grown in isolation and grown with (b) Vaccinium myrtillus or (c) Vaccinium vitis-idaea. Ericaceous plants did not act as a species pool of pine mycobionts and inhibited the ability of the potentially shared species Meliniomyces bicolor to form ectomycorrhizae. Similarly, Ericaceae significantly reduced the formation of Thelephora terrestris ectomycorrhizae in P. sylvestris. EcMF species composition in the mesocosms was strongly affected by both the host species and the presence of an ericaceous neighbour. When grown in isolation, P. strobus root tips were predominantly colonised by Wilcoxina mikolae, whereas those of P. sylvestris were more commonly colonised by Suillus and Rhizopogon spp. Interestingly, these differences were less evident (Suillus + Rhizopogon spp.) or absent (W. mikolae) when the pines were grown with Ericaceae. P. strobus exclusively associated with Rhizopogon salebrosus s.l., suggesting the presence of host specificity at the intrageneric level. Ericaceous plants had a positive effect on colonisation of P. strobus root tips by R. salebrosus s.l. This study demonstrates that the interaction of selective factors such as host species and presence of ericaceous plants may affect the realised niche of the ectomycorrhizal fungi.
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19.
  • Kytöviita, Minna-Maarit (författare)
  • Role of nutrient level and defoliation on symbiotic function: experimental evidence by tracing C-14/N-15 exchange in mycorrhizal birch seedlings
  • 2005
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 1432-1890 .- 0940-6360. ; 15:1, s. 65-70
  • Tidskriftsartikel (refereegranskat)abstract
    • High nutrient availability and defoliation generally reduce ectomycorrhizal colonization levels in trees, but it is not known how this affects the functional aspects of mycorrhizal symbiosis. It was therefore investigated whether (1) defoliation or increasing substrate N availability reduce C allocation from the plant to the fungus and N allocation from the fungus to the plant (symbiotic resource exchange), (2) symbiotic resource exchange depends on relative N and P availability, and (3) fungal N translocation to plant and plant C allocation to fungus are interdependent. Birch (Betula pendula) seedlings were grown in symbiosis with the ectomycorrhizal fungus Paxillus involutus at five times excess N, or at five times excess N and P for 6 weeks. One-half of the plants were defoliated and the plant shoots were allowed to photosynthesize (CO2)-C-14 while the fungal compartment was exposed to (NH4)-N-14. After 3 days, the C-14 of plant origin in fungal tissues and N-15 of fungal origin in plant tissues were quantified. Nutrient availability had no observable effect on symbiotic resource exchange in non-defoliated systems. Defoliation reduced symbiotic N acquisition by plants at all levels of nutrient availability, with the reduction being most marked at higher N availability, indicating an increased tendency in the symbiotic system to discontinue resource exchange after defoliation at higher fertility levels. The concentration of C-14 in extramatrical mycelium correlated significantly with the concentration of N-15 in birch shoots. The results support the assumption that N delivery to the host by the mycorrhizal fungus is dependent on C flow from the plant to the fungus, and that exchanges between the partners are reciprocal. No significant reductions in root C-14 content as a response to defoliation were observed, indicating that defoliation specifically reduced allocation to fungus, but not markedly to roots.
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20.
  • Lithell, Cajsa, et al. (författare)
  • Localisation of phosphomonoesterase activity in ectomycorrhizal fungi grown on different phosphorus sources
  • 2009
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 19, s. 197-204
  • Tidskriftsartikel (refereegranskat)abstract
    • Phosphorus (P) is a major limiting nutrient for plants in boreal forest ecosystems where a substantial part of the total P is sequestered in organic compounds. Some ectomycorrhizal (ECM) fungi are known to produce phosphomonoesterases, enzymes that degrade organic P sources. Here, we test 16 ECM species for this enzymatic activity by growing them on media containing orthophosphate, phytic acid or apatite. A method with an overlay gel that determined both phosphomonoesterase activity and its spatial distribution was developed. The phosphomonoesterase activity was not significantly higher when growing on organic P; conversely some isolates only produced measurable enzyme activity when grown on apatite. Species-specific variations with respect to phosphomonoesterase activity as well as growth responses to different substrates were found. The production of phosphomonoesterases was found to be widespread in ECM fungi and the enzyme activity did not need induction by organic P. The enzyme activity was highest in the central parts of the mycelia, potentially reflecting breakdown and recycling of phospholipids from old hyphae or potentially higher mycelial density.
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21.
  • Menkis, A, et al. (författare)
  • Afforestation of abandoned farmland with conifer seedlings inoculated with three ectomycorrhizal fungi - impact on plant performance and ectomycorrhizal community.
  • 2007
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 17:4, s. 337-348
  • Tidskriftsartikel (refereegranskat)abstract
    • The aim of a 3-year study was to investigate whether inoculation of Pinus sylvestris L. and Picea abies (L.) Karst. seedlings with mycorrhizas of Cenococcum geophilum Fr., Piceirhiza bicolorata, and Hebeloma crustuliniforme (Bull.) Quel. has any impact on: 1) survival and growth of outplanted seedlings on abandoned agricultural land, and 2) subsequent mycorrhizal community development. For inoculation, the root system of each plant was wrapped in a filter paper containing mycelium, overlaid with damp peat-sand mixture and wrapped in a paper towel. In total, 8,000 pine and 8,000 spruce seedlings were planted on 4-ha of poor sandy soil in randomized blocks. Already after the first year natural mycorrhizal infections prevailed in the inoculated root systems, and introduced mycorrhizas were seldom found. Yet, the seedlings that had been pre-inoculated with C. geophilum and the P. bicolorata during the whole 3-year period showed significantly higher survival and growth as compared to controls. Moreover, the independent colonization of roots by C. geophilum and the P. bicolorata from natural sources was also observed. A diverse mycorrhizal community was detected over two growing seasons in all treatments, showing low impact of inoculation on subsequent fungal community development. A total of 19 additional ectomycorrhizal morphotypes was observed, which clustered into two well-separated groups, according to host tree species (pine and spruce). In conclusion, the results showed limited ability to increase tree survival and growth, and to manipulate the mycorrhizal community even by extensive pre-inoculations, indicating that fungal community formation in root systems is governed mainly by environmental factors.
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22.
  • Menkis, Audrius, et al. (författare)
  • Effects of stump and slash removal on growth and mycorrhization of Picea abies seedlings outplanted on a forest clear-cut
  • 2010
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 20, s. 505-509
  • Tidskriftsartikel (refereegranskat)abstract
    • The objectives of this study were to investigate impact of stump and slash removal on growth and mycorrhization of Picea abies seedlings outplanted on a forest clear-cut. Four non-replicated site preparation treatments included: (1) mounding (M), (2) removal of stumps (K), (3) mounding and removal of logging slash (HM) and (4) removal of logging slash and stumps (HK). Results showed that height increment of the seedlings was highest in K and lowest in M after the third growing season, and similar pattern remained after the fourth season. Ectomycorrhizal (ECM) colonisation of seedling roots was highest in M (96.6%) and lowest in K (72.3%), and even in HK (76.0%) and HM (76.3%). Morphotyping and sequencing of internal transcribed spacer of fungal ribosomal DNA revealed a total of 13 ECM species. Among those, Thelephora terrestris and Cenococcum geophilum were the most common, found on 27.4% and 26.3% of roots, respectively. The rest of species colonised 26.6% of roots. Richness of ECM species was highest in M (10 species) and lowest in K (three species). Consequently, stump and slash removal from clear-felled sites had a positive effect on growth of outplanted spruce seedlings, but negative effect on their mycorrhization. This suggests that altered soil conditions due to site disturbance by stump and slash removal might be more favourable for tree growth than more abundant mycorrhization of their root systems in less disturbed soil.
  •  
23.
  • Menkis, Audrius (författare)
  • The ectomycorrhizal community of conifer stands on peat soils 12 years after fertilization with wood ash
  • 2016
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 26, s. 153-160
  • Tidskriftsartikel (refereegranskat)abstract
    • We studied long-term effects of fertilization with wood ash on biomass, vitality and mycorrhizal colonization of fine roots in three conifer forest stands growing in Vacciniosa turf. mel. (V), Myrtillosa turf. mel. (M) and Myrtillosa turf. mel./Caricoso-phragmitosa (MC) forest types on peat soils. Fertilization trials amounting 5 kg/m(2) of wood ash were established 12 years prior to this study. A total of 63 soil samples with roots were collected and analysed. Ectomycorrhizal (ECM) fungi in roots were identified by morphotyping and sequencing of the fungal internal transcribed spacer (ITS) region. In all forest types, fine root biomass was higher in fertilized plots than in control plots. In M forest type, proportion of living fine roots was greater in fertilized plots than in control plots, while in V and MC, the result was opposite. Fifty ECM species were identified, of which eight were common to both fertilized and control plots. Species richness and Shannon diversity index were generally higher in fertilized plots than in control plots. The most common species in fertilized plots were Amphinema byssoides (17.8 %) and Tuber cf. anniae (12.2 %), while in control plots, it was Tylospora asterophora (18.5 %) and Lactarius tabidus (20.3 %). Our results showed that forest fertilization with wood ash has long-lasting effect on diversity and composition of ECM fungal communities.
  •  
24.
  • Moora, Mari, et al. (författare)
  • AM fungal communities inhabiting the roots of submerged aquatic plant Lobelia dortmanna are diverse and include a high proportion of novel taxa
  • 2016
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 26:7, s. 735-745
  • Tidskriftsartikel (refereegranskat)abstract
    • While the arbuscular mycorrhizal (AM) symbiosis is known to be widespread in terrestrial ecosystems, there is growing evidence that aquatic plants also form the symbiosis. It has been suggested that symbiosis with AM fungi may represent an important adaptation for isoA << tid plants growing on nutrient-poor sediments in oligotrophic lakes. In this study, we address AM fungal root colonization intensity, richness and community composition (based on small subunit (SSU) ribosomal RNA (rRNA) gene sequencing) in five populations of the isoA << tid plant species Lobelia dortmanna inhabiting oligotrophic lakes in Southern Sweden. We found that the roots of L. dortmanna hosted rich AM fungal communities and about 15 % of the detected molecular taxa were previously unrecorded. AM fungal root colonization intensity and taxon richness varied along an environmental gradient, being higher in oligotrophic and lower in mesotrophic lakes. The overall phylogenetic structure of this aquatic fungal community differed from that described in terrestrial systems: The roots of L. dortmanna hosted more Archaeosporaceae and fewer Glomeraceae taxa than would be expected based on global data from terrestrial AM fungal communities.
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25.
  • Mundra, Sunil, et al. (författare)
  • Alpine bistort (Bistorta vivipara) in edge habitat associates with fewer but distinct ectomycorrhizal fungal species : a comparative study of three contrasting soil environments in Svalbard
  • 2016
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 26:8, s. 809-818
  • Tidskriftsartikel (refereegranskat)abstract
    • Bistorta vivipara is a widespread arctic-alpine ectomycorrhizal (ECM) plant species. Recent findings suggest that fungal communities associated with B. vivipara roots appear random over short distances, but at larger scales, environmental filtering structure fungal communities. Habitats in highly stressful environments where specialist species with narrower niches may have an advantage represent unique opportunity to test the effect of environmental filtering. We utilised high-throughput amplicon sequencing to identify ECM communities associated with B. vivipara in Svalbard. We compared ECM communities in a core habitat where B. vivipara is frequent (Dryas-heath) with edge habitats representing extremes in terms of nutrient availability where B. vivipara is less frequent (bird-manured meadow and a nutrient-depleted mine tilling). Our analysis revealed that soil conditions in edge habitats favour less diverse but more distinct ECM fungal communities with functional traits adapted to local conditions. ECM richness was overall lower in both edge habitats, and the taxonomic compositions of ECM fungi were in line with our functional expectations. Stress-tolerant genera such as Laccaria and Hebeloma were abundant in nutrient-poor mine site whereas functional competitors genera such as Lactarius and Russula were dominant in the nutrient-rich bird-cliff site. Our results suggest that ECM communities in rare edge habitats are most likely not subsets of the larger pool of ECM fungi found in natural tundra, and they may represent a significant contribution to the overall diversity of ECM fungi in the Arctic.
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26.
  • Oja, Jane, et al. (författare)
  • Local-scale spatial structure and community composition of orchid mycorrhizal fungi in semi-natural grasslands
  • 2017
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 27:4, s. 355-367
  • Tidskriftsartikel (refereegranskat)abstract
    • Orchid mycorrhizal (OrM) fungi play a crucial role in the ontogeny of orchids, yet little is known about how the structure of OrM fungal communities varies with space and environmental factors. Previous studies suggest that within orchid patches, the distance to adult orchids may affect the abundance of OrM fungi. Many orchid species grow in species-rich temperate semi-natural grasslands, the persistence of which depends on moderate physical disturbances, such as grazing and mowing. The aim of this study was to test whether the diversity, structure and composition of OrM fungal community are influenced by the orchid patches and management intensity in semi-natural grasslands. We detected putative OrM fungi from 0 to 32 m away from the patches of host orchid species (Orchis militaris and Platanthera chlorantha) in 21 semi-natural calcareous grasslands using pyrosequencing. In addition, we assessed different ecological conditions in semi-natural grasslands but primarily focused on the effect of grazing intensity on OrM fungal communities in soil. We found that investigated orchid species were mostly associated with Ceratobasidiaceae and Tulasnellaceae and, to a lesser extent, with Sebacinales. Of all the examined factors, the intensity of grazing explained the largest proportion of variation in OrM fungal as well as total fungal community composition in soil. Spatial analyses showed limited evidence for spatial clustering of OrM fungi and their dependence on host orchids. Our results indicate that habitat management can shape OrM fungal communities, and the spatial distribution of these fungi appears to be weakly structured outside the orchid patches.
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27.
  • Olsson, Ola, et al. (författare)
  • Phosphorus and carbon availability regulate structural composition and complexity of AM fungal mycelium.
  • 2014
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 1432-1890 .- 0940-6360. ; 24:6, s. 443-451
  • Tidskriftsartikel (refereegranskat)abstract
    • The regulation of the structural composition and complexity of the mycelium of arbuscular mycorrhizal (AM) fungi is not well understood due to their obligate biotrophic nature. The aim of this study was to investigate the structure of extraradical mycelium at high and low availability of carbon (C) to the roots and phosphorus (P) to the fungus. We used monoxenic cultures of the AM fungus Rhizophagus irregularis (formerly Glomus intraradices) with transformed carrot roots as the host in a cultivation system including a root-free compartment into which the extraradical mycelium could grow. We found that high C availability increased hyphal length and spore production and anastomosis formation within individual mycelia. High P availability increased the formation of branched absorbing structures and reduced spore production and the overall length of runner hyphae. The complexity of the mycelium, as indicated by its fractal dimensions, increased with both high C and P availability. The results indicate that low P availability induces a growth pattern that reflects foraging for both P and C. Low C availability to AM roots could still support the explorative development of the mycelium when P availability was low. These findings help us to better understand the development of AM fungi in ecosystems with high P input and/or when plants are subjected to shading, grazing or any management practice that reduces the photosynthetic ability of the plant.
  •  
28.
  • Rineau, F., et al. (författare)
  • Comparative genomics and expression levels of hydrophobins from eight mycorrhizal genomes
  • 2017
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 27:4, s. 383-396
  • Tidskriftsartikel (refereegranskat)abstract
    • Hydrophobins are small secreted proteins that are present as several gene copies in most fungal genomes. Their properties are now well understood: they are amphiphilic and assemble at hydrophilic/hydrophobic interfaces. However, their physiological functions remain largely unexplored, especially within mycorrhizal fungi. In this study, we identified hydrophobin genes and analysed their distribution in eight mycorrhizal genomes. We then measured their expression levels in three different biological conditions (mycorrhizal tissue vs. free-living mycelium, organic vs. mineral growth medium and aerial vs. submerged growth). Results confirmed that the size of the hydrophobin repertoire increased in the terminal orders of the fungal evolutionary tree. Reconciliation analysis predicted that in 41% of the cases, hydrophobins evolved from duplication events. Whatever the treatment and the fungal species, the pattern of expression of hydrophobins followed a reciprocal function, with one gene much more expressed than others from the same repertoire. These most-expressed hydrophobin genes were also among the most expressed of the whole genome, which suggests that they play a role as structural proteins. The fine-tuning of the expression of hydrophobin genes in each condition appeared complex because it differed considerably between species, in a way that could not be explained by simple ecological traits. Hydrophobin gene regulation in mycorrhizal tissue as compared with free-living mycelium, however, was significantly associated with a calculated high exposure of hydrophilic residues.
  •  
29.
  • Ryberg, Martin, 1976, et al. (författare)
  • Weak habitat specificity in ectomycorrhizal communities associated with Salix herbacea and Salix polaris in alpine tundra
  • 2011
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. ; 21:4, s. 289-96
  • Tidskriftsartikel (refereegranskat)abstract
    • This study explores mid-alpine ectomycorrhizal communities on Salix herbacea and Salix polaris in plant communities differing in nutrient status and snow conditions. Plant species were identified by tracking roots back to above ground structures while fungal species were identified using molecular methods. The fungi were identified to 34 molecular operational taxonomic units (MOTUs)/species but species accumulation curves indicated that the communities were only partially sampled. The estimated total species richness was 49 (±9 SD) MOTUs/ species. No significant ectomycorrhizal community specificity was found between the two plant species and only weak specificity between different plant communities. Furthermore, no difference in proportion of colonized root tips could be demonstrated between plant communities. However, some fungal taxa showed tendencies to associate with specific environmental conditions. Sebacinaceae, Inocybe egenula, Russula cf.emetica, and a Tomentella sp. were found in meadow communities but not in the heath communities. Sistotrema cf. alboluteum and Tomentella cf. terrestris were only found in the dry and mesic heath communities. Classifications into exploration types showedthat the contact type is more abundant in the dry heath community than the other communities. Cenococcum geophilum was the most common species but Cortinarius spp., Russula spp., Tomentella spp., and Lactarius spp. were also common. This study confirms that alpine communities are rich in ectomycorrhizal fungi including species from a wide variety of fungal lineages and also show that many dominant species have wide ecological amplitude.
  •  
30.
  • Saitta, Alessandro, et al. (författare)
  • Tree species identity and diversity drive fungal richness and community composition along an elevational gradient in a Mediterranean ecosystem
  • 2018
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 28:1, s. 39-47
  • Tidskriftsartikel (refereegranskat)abstract
    • Ecological and taxonomic knowledge is important for conservation and utilization of biodiversity. Biodiversity and ecology of fungi in Mediterranean ecosystems is poorly understood. Here, we examined the diversity and spatial distribution of fungi along an elevational gradient in a Mediterranean ecosystem, using DNA metabarcoding. This study provides novel information about diversity of all ecological and taxonomic groups of fungi along an elevational gradient in a Mediterranean ecosystem. Our analyses revealed that among all biotic and abiotic variables tested, host species identity is the main driver of the fungal richness and fungal community composition. Fungal richness was strongly associated with tree richness and peaked in Quercus-dominated habitats and Cistus-dominated habitats. The highest taxonomic richness of ectomycorrhizal fungi was observed under Quercus ilex, whereas the highest taxonomic richness of saprotrophs was found under Pinus. Our results suggest that the effect of plant diversity on fungal richness and community composition may override that of abiotic variables across environmental gradients.
  •  
31.
  • Schnoor, Tim Krone, et al. (författare)
  • Mechanical soil disturbance as a determinant of arbuscular mycorrhizal fungal communities in semi-natural grassland.
  • 2011
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 1432-1890 .- 0940-6360. ; 21:3, s. 211-220
  • Tidskriftsartikel (refereegranskat)abstract
    • While the effect of disturbance on overall abundance and community composition of arbuscular mycorrhizal (AM) fungi has been researched in agricultural fields, less is known about the impact in semi-natural grasslands. We sampled two AM plant species, Festuca brevipila and Plantago lanceolata, from an ongoing grassland restoration experiment that contained replicated plowed and control plots. The AM fungal community in roots was determined using nested PCR and LSU rDNA primers. We identified 38 phylotypes within the Glomeromycota, of which 29 belonged to Glomus A, six to Glomus B, and three to Diversisporaceae. Only three phylotypes were closely related to known morphospecies. Soil disturbance significantly reduced phylotype richness and changed the AM fungal community composition. Most phylotypes, even closely related ones, showed little or no overlap in their distribution and occurred in either the control or disturbed plots. We found no evidence of host preference in this system, except for one phylotype that preferentially seemed to colonize Festuca. Our results show that disturbance imposed a stronger structuring force for AM fungal communities than did host plants in this semi-natural grassland.
  •  
32.
  • Timonen, Sari, et al. (författare)
  • Dynamics of cytoskeletal proteins in developing pine ectomycorrhiza
  • 1996
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 1432-1890 .- 0940-6360. ; 6:5, s. 423-429
  • Tidskriftsartikel (refereegranskat)abstract
    • Mycorrhizal short roots of Pinus contorta Dougl. ex Loud colonized by Suillus variegatus (Sow. ex Fr.) O. Kuntze or Paxillus involutus (Batsch) Fr. were collected 1->60 days after fungal contact. The proteins of the inoculated roots were extracted, electrophoretically separated, blotted and immunostained for alpha-tubulin and actin. The development of the mycorrhiza was also followed microscopically. The signal of plant alpha-tubulin was stronger than the signal of fungal alpha-tubulin during the first 5 days in S. variegatus mycorrhiza and was then exceeded by fungal alpha-tubulin. This correlated well with the increase of fungal mycelium in the mycorrhiza. A transient drop in both plant and fungal alpha-tubulin signals was observed 20 days after fungal contact, suggesting a change in the metabolism of the mycorrhiza. The signals for plant and fungal actins in the mycorrhiza increased steadily during early infection and then remained at a high level as the mycorrhiza matured. Similar trends were observed in P. contorta-P. involutus mycorrhiza. The data from P. contorta-S. variegatus mycorrhizas suggests that alpha-tubulin is a growth-related protein, subject to changes, while the amount of actin reflects the general metabolic activity of the mycorrhiza. In both mycorrhizal systems clear alpha-tubulin and actin signals were detected 60 days after colonization, which indicates that the mycorrhizas were metabolically active in spite of their withered appearance.
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33.
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34.
  • Vasaitis, Rimvydas, et al. (författare)
  • Impact of Heterobasidion root-rot on fine root morphology and associated fungi in Picea abies stands on peat soils
  • 2016
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 26, s. 465-473
  • Tidskriftsartikel (refereegranskat)abstract
    • We examined differences in fine root morphology, mycorrhizal colonisation and root-inhabiting fungal communities between Picea abies individuals infected by Heterobasidion root-rot compared with healthy individuals in four stands on peat soils in Latvia. We hypothesised that decreased tree vitality and alteration in supply of photosynthates belowground due to root-rot infection might lead to changes in fungal communities of tree roots. Plots were established in places where trees were infected and in places where they were healthy. Within each stand, five replicate soil cores with roots were taken to 20 cm depth in each root-rot infected and uninfected plot. Root morphological parameters, mycorrhizal colonisation and associated fungal communities, and soil chemical properties were analysed. In three stands root morphological parameters and in all stands root mycorrhizal colonisation were similar between root-rot infected and uninfected plots. In one stand, there were significant differences in root morphological parameters between root-rot infected versus uninfected plots, but these were likely due to significant differences in soil chemical properties between the plots. Sequencing of the internal transcribed spacer of fungal nuclear rDNA from ectomycorrhizal (ECM) root morphotypes of P. abies revealed the presence of 42 fungal species, among which ECM basidiomycetes Tylospora asterophora (24.6 % of fine roots examined), Amphinema byssoides (14.5 %) and Russula sapinea (9.7 %) were most common. Within each stand, the richness of fungal species and the composition of fungal communities in root-rot infected versus uninfected plots were similar. In conclusion, Heterobasidion root-rot had little or no effect on fine root morphology, mycorrhizal colonisation and composition of fungal communities in fine roots of P. abies growing on peat soils.
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35.
  • Wallander, Håkan, et al. (författare)
  • Ectomycorrhizal fungal biomass in roots and uptake of P from apatite by Pinus sylvestris seedlings growing in forest soil with and without wood ash amendment
  • 2005
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 1432-1890 .- 0940-6360. ; 15:2, s. 143-148
  • Tidskriftsartikel (refereegranskat)abstract
    • Forest soil from an experimental Norway spruce forest with four levels of wood ash addition (0, 1, 3 and 6 tonnes ha(-1)) was used to inoculate pine (Pinus sylvestris) seedlings with indigenous ectomycorrhizal (EM) fungi. Uptake of P-32 and Rb-86 in a root bioassay was used to estimate the demand for P and K by seedlings grown in the different soils. Utilisation of P from apatite was tested in a laboratory system where uptake by the ectomycorrhizal mycelium was separated from uptake by roots. The demand for P and K in the seedlings was similar regardless of the ash treatment. Variation in EM levels, estimated as fungal biomass (ergosterol) in roots, was large in the different soils, but not related to ash addition. Uptake of P from apatite was, on average, 23% of total seedling P and was not related to EM levels. It was concluded that the improved P uptake from apatite by EM fungi found in earlier studies is probably not a general phenomenon among EM fungi. The small effect of ash addition on EM levels and P uptake suggests that addition of granulated wood ash is a forest management treatment that will have only minor influence on ectomycorrhizal symbiosis.
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36.
  • Wallander, Håkan, et al. (författare)
  • Limited transfer of nitrogen between wood decomposing and ectomycorrhizal mycelia when studied in the field
  • 2006
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 1432-1890 .- 0940-6360. ; 16:3, s. 213-217
  • Tidskriftsartikel (refereegranskat)abstract
    • Transfer of N-15 between interacting mycelia of a wood-decomposing fungus (Hypholoma fasciculare) and an ectomycorrhizal fungus (Tomentellopsis submollis) was studied in a mature beech (Fagus sylvatica) forest. The amount of N-15 transferred from the wood decomposer to the ectomycorrhizal fungus was compared to the amount of N-15 released from the wood-decomposing mycelia into the soil solution as N-15-NH4. The study was performed in peat-filled plastic containers placed in forest soil in the field. The wood-decomposing mycelium was growing from an inoculated wood piece and the ectomycorrhizal mycelium from an introduced root from a mature tree. The containers were harvested after 41 weeks when physical contact between the two foraging mycelia was established. At harvest, N-15 content was analyzed in the peat (total N and (NH4+)-N-15) and in the mycorrhizal roots. A limited amount of N-15 was transferred to the ectomycorrhizal fungus and this transfer could be explained by (NH4+)-N-15 released from the wood-decomposing fungus without involving any antagonistic interactions between the two mycelia. Using our approach, it was possible to study nutritional interactions between basidiomycete mycelia under field conditions and this and earlier studies suggest that the outcomes of such interactions are highly species-specific and depend on environmental conditions such as resource availability.
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37.
  • Wallander, Håkan, et al. (författare)
  • Relationships between fungal uptake of ammonium, fungal growth and nitrogen availability in ectomycorrhizal Pinus sylvestris seedlings
  • 1999
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 1432-1890 .- 0940-6360. ; 8:4, s. 215-223
  • Tidskriftsartikel (refereegranskat)abstract
    • Nitrogen deposition and intentional forest fertilisation with nitrogen are known to affect the species composition of ectomycorrhizal fungal communities. To learn more about the mechanisms responsible for these effects, the relations between fungal growth, nitrogen uptake and nitrogen availability were studied in ectomycorrhizal fungi in axenic cultures and in symbiosis with pine seedlings. Effects of different levels of inorganic nitrogen (NH4) on the mycelial growth of four isolates of Paxillus involutus and two isolates of Suillus bovinus were assessed. With pine seedlings, fungal uptake of N-15-labelled NH4 was studied in shortterm incubation experiments (72 h) in microcosms and in long-term incubation experiments (3 months) in pot cultures. For P. involutus growing in symbiosis with pine seedlings, isolates with higher NH4 uptake were affected more negatively at high levels of nitrogen availability than isolates with lower uptake. More NH4 was allocated to shoots of seedlings colonised by a high-uptake isolate, indicating transfer of a larger fraction of assimilated NH4 to the host than with isolates showing lower NH4 uptake rates. Thus low rates of N uptake and N transfer to the host may enable EM fungi avoid stress induced by elevated levels of nitrogen. Seedlings colonised by S. bovinus transferred a larger fraction of the N-15 label to the shoots than seedlings colonised by P. involutus. Seedling shoot growth probably constituted a greater carbon sink in pot cultures than in microcosms, since the mycelial growth of P. involutus was more sensitive to high NH4 in pots. There was no homology in mycelial growth rate between pare culture and growth in symbiosis, but N uptake in pure culture corresponded to that during growth in symbiosis. No relationship was found between deposition of antropogenic nitrogen at the sites of origin of the P. involutus isolates and their mycelial growth or uptake of inorganic nitrogen
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38.
  • Wedén, Christina, et al. (författare)
  • The population of the hypogeous fungus Tuber aestivum syn T. uncinatum on the island of Gotland
  • 2004
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 14:1, s. 19-23
  • Tidskriftsartikel (refereegranskat)abstract
    • The aim of our study was to examine the genetic variation within Tuber aestivum on the Baltic island of Gotland, Sweden. Variation in such a limited geographical area should help illuminate the dispersal abilities of T. aestivum. Knowledge of the genetic variation in this northern outpost could also be useful in the selection of inoculum for the establishment of truffle orchards. Genetic structure and homogeneity of the population were studied using principal component and parsimony analyses of randomly amplified polymorphic DNA data. Our inventories showed that T. aestivum is abundantly distributed in suitable habitats on Gotland. The genetic variation observed suggests sexual reproduction and slow dispersal on the island. It is possible that the present population was established from one introduction, which may be due to ability to survive in this habitat rather than to rare colonising events. The T. aestivum population on Gotland may be an ecotype adapted to the climate and soil conditions on the island.
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39.
  • Zhang, Ke (författare)
  • Fusarium oxysporum KB-3 from Bletilla striata: an orchid mycorrhizal fungus
  • 2019
  • Ingår i: Mycorrhiza. - : Springer Science and Business Media LLC. - 0940-6360 .- 1432-1890. ; 29, s. 531-540
  • Tidskriftsartikel (refereegranskat)abstract
    • Orchid mycorrhizal fungi are essential for the seed germination and vegetative growth of orchids. The orchid Bletilla striata has great medical value in China because its tuber is rich in mannan. Some endophytic fungi were isolated from the roots of B. striata. The isolate KB-3 was selected for experiments because it could promote the germination of B. striata seeds. Based on morphological characters and phylogenetic analysis, the isolate KB-3 was identified as Fusarium oxysporum. Co-cultivation experiments of KB-3 with B. striata and Dendrobium candidum were performed to demonstrate orchid mycorrhizal structures. Microscopic examination showed that KB-3 established colonization and produced coiled hyphal structures known as pelotons within the cortical cells of both orchid roots. The results confirm that F. oxysporum KB-3 can behave as an orchid mycorrhizal fungus.
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40.
  • Andreasson, Frida (författare)
  • Comparison of ingrowth cores and ingrowth meshes in root studies: 3 years of data on Pinus pinaster and its understory
  • 2016
  • Ingår i: Trees - Structure and Function. - : Springer Science and Business Media LLC. - 0931-1890 .- 1432-2285. ; 30, s. 555-570
  • Tidskriftsartikel (refereegranskat)abstract
    • Key message Pine fine root biomass, live fraction and specific root length studied after collection with ingrowth cores do not correspond with those collected with ingrowth meshes. Abstract Root studies are a challenging task, yet they are primordial to improve our understanding of biogeochemical cycles in many ecosystems. Methods reducing operator time and minimizing disturbance while permitting adequate assessment of root dimensions in space and time would naturally be of great relevance. Recently, ingrowth meshes have been proposed as a valuable alternative to existing methods, deployable in remote areas and potentially yielding smaller disturbances along with a reduction in time spent per sample. In this study, we used flexible mesh material in Pinus pinaster production forests. The mesh material (pore size 6 by 6 mm) was either applied as cores (15 cm deep, 8 cm diameter) or as linear meshes (15 cm deep, 50 cm long), and left in soils for 9, 24, or 36 months. Roots were retrieved from both devices and separated over plant species (pine and understory species), diameter class and vitality (live or dead). We expected 1) improved assessment of larger diameter roots in the linear meshes due to a larger mesh surface and 2) less disturbance of the soil for the linear meshes. Results indicate that most patterns were similar between both devices and species, but the meshes resulted in lower ingrowth for all diameter classes, and the cores better reflected the standing biomass. The larger surface of the linear meshes resulted in larger disturbance and did not permit to reduce time per sample. Future research may target ingrowth meshes of reduced size and perhaps of more rigid material.
  •  
41.
  • Arnebrant, Kristina (författare)
  • Nitrogen amendments reduce the growth of extramatrical ectomycorrhizal mycelium
  • 1994
  • Ingår i: Mycorrhiza. - 1432-1890. ; 5:1, s. 7-15
  • Tidskriftsartikel (refereegranskat)abstract
    • The effect of three different nitrogen sources on the growth of external ectomycorrhizal mycelium was studied in Perspex micorocosms. Nonsterile peat was used as substrate. Five different fungal isolates growing in symbiosis with pine seedlings were investigated: two isolates of Paxillus involutus, one of Suillus bovinus and two unidentified ectomycorrhizal fungi isolated from ectomycorrhizal root tips. Three different nitrogen sources were used: ammonium as (NH4)2SO4, nitrate as NaNO3 and a complete nutrient solution (Ingestad 1979), and three different nitrogen concentrations, 1, 2 or 4 mg N/g dry wt. of peat. The mycelial growth of all fungi was found to be negatively affected by the nitrogen amendments, although the sensitivity to nitrogen varied between the isolates. One of the unidentified isolates was extremely sensitive and growth was completely inhibited by all nitrogen treatments. In contrast, the growth of one of the P. involutus isolates was only slightly reduced by the nitrogen amendments. The different nitrogen sources all reduced growth, and since no significant difference was found between the nitrogen sources or between the different nitrogen concentrations the results were pooled to give one value that summarized the effect of nitrogen on mycelial growth. Thus, the mycelial growth of one of the two P. involutus isolates was reduced to approximately 80% of the growth in the control, the other P. involutus and one of the unidentified fungi, vgk 2 89.10, were reduced to 40–50% of the control growth, S. bovinus to 30% of the control and the most sensitive fungus, the unidentified isolate vg 1 87.10, was reduced to 3% of the growth in the control treatment. In all experiments, the shoot to root ratio generally increased, mainly as a result of increased shoot growth.
  •  
42.
  • Beyer, Friderike, et al. (författare)
  • Relationship between foliar δ13C and sapwood area indicates different water use patterns across 236 Salix genotypes
  • 2018
  • Ingår i: Trees. - : Springer Science and Business Media LLC. - 0931-1890 .- 1432-2285. ; 32:6, s. 1737-1750
  • Tidskriftsartikel (refereegranskat)abstract
    • The relationship between leaf δ13C and plant size (represented by e.g. total leaf area) has been used to analyze different water use patterns of plants. However, the total leaf area (TLA) is difficult to assess in trees. Our aims were to (i) identify a feasible predictor for TLA; (ii) estimate the effects of TLA on leaf-level δ13C and δ18O values; and (iii) evaluate whether the relationship between leaf-level δ13C and a TLA proxy can be used to discriminate between different water use patterns. Various leaf and shoot traits of up to 236 Salix genotypes field-grown in Sweden and Italy were assessed and analyzed. Accumulated shoot diameter and sapwood area (SA) calculated from it were the best predictors for TLA. The SA was significantly correlated with foliar δ13C, but not δ18O values in some genotypes. The effects of SA on foliar δ13C values varied significantly among genotypes, and the foliar δ13C–SA relationship could be used to discriminate between different water use patterns across 236 Salix genotypes. Our results demonstrate a great variability of water use patterns across taxonomically closely related plants, and may also have implications for Salix pre-breeding and selection for different drought conditions.
  •  
43.
  • Bonosi, Lorenzo, et al. (författare)
  • Growth responses of 15 Salix genotypes to temporary water stress are different from the responses to permanent water shortage
  • 2010
  • Ingår i: Trees - Structure and Function. - : Springer Science and Business Media LLC. - 0931-1890 .- 1432-2285. ; 24, s. 843-854
  • Tidskriftsartikel (refereegranskat)abstract
    • Hybrids of willow (Salix spp.) are today grown as biomass crops for energy in several cool-temperate regions including Sweden. These trees have great growth potential also under warmer climates, but may suffer from drought and heat. Research on tree growth under drought has often focused on the responses to permanent water stress. Less attention has been paid to the effects of temporary water stress on tree growth, although periods of strong water shortage are recurrent in many regions of the world. We performed a greenhouse experiment to assess the impact of five treatments including permanent (PWS; 30 days of water shortage) and temporary (TWS; 4-12 days of water shortage followed by normal watering) water stress on growth and leaf traits of 15 Salix genotypes. Plant biomass and height increment decreased across treatments in the order control (well-watered) > TWS > PWS, but there was also a significant genotype x treatment interaction. The pattern in growth traits was not directly reflected by the leaf traits, for which there was no significant genotype x treatment interaction (except for leaf angle). The significant genotype x treatment interactions for growth traits indicate a potential for breeding. Permanent water shortage does not necessarily represent an appropriate environment for the selection of genotypes for environments characterised by temporary water stress.
  •  
44.
  • Canales, Javier, et al. (författare)
  • Gene expression profiling in the stem of young maritime pine trees : detection of ammonium stress-responsive genes in the apex
  • 2011
  • Ingår i: Trees. - : Springer Science and Business Media LLC. - 0931-1890 .- 1432-2285. ; 26:2, s. 609-619
  • Tidskriftsartikel (refereegranskat)abstract
    • The shoots of young conifer trees represent an interesting model to study the development and growth of conifers from meristematic cells in the shoot apex to differentiated tissues at the shoot base. In this work, microarray analysis was used to monitor contrasting patterns of gene expression between the apex and the base ofmaritime pine shoots. A group of differentially expressed genes were selected and validated by examining their relative expression levels in different sections along thestem, from the top to the bottom. After validation of the microarray data, additional geneexpression analyses were also performed in the shoots of young maritime pine treesexposed to different levels of ammonium nutrition. Our results show that the apex ofmaritime pine trees is extremely sensitive to conditions of ammonium excess or deficiency, as revealed by the observed changes in the expression of stress-responsivegenes. This new knowledge may be used to precocious detection of early symptoms of nitrogen nutritional stresses, thereby increasing survival and growth rates of young treesin managed forests. 
  •  
45.
  • Carlsson, Johanna, et al. (författare)
  • Nitrogen utilization during germination of somatic embryos of Norway spruce : revealing the importance of supplied glutamine for nitrogen metabolism
  • 2019
  • Ingår i: Trees. - : SPRINGER HEIDELBERG. - 0931-1890 .- 1432-2285. ; 33:2, s. 383-394
  • Tidskriftsartikel (refereegranskat)abstract
    • Key messageThis paper shows that germinating Norway spruce somatic embryos are dependent on the carbon and nitrogen supplied in the medium, and that supplied glutamine accounts for 50 % of assimilated nitrogen during germination.AbstractThe female megagametophyte, which provides the zygotic embryo with nitrogen (N), carbon (C) and energy during germination, is not present in Norway spruce (Picea abies) mature somatic embryos. Therefore, somatic embryos presumably rely on nutrients supplied in the germination medium in addition to their storage compounds accumulated during maturation. However, to what extent stored versus supplied compounds contribute to a somatic embryo germination is unclear. In this 24-day study, we addressed the above question by monitoring the biomass changes and the N and C budget during somatic embryo germination, under low-intensity red light. We found that the C and N storage reserves, accumulated during the maturation phase, were not sufficient to support the growth of the germinating somatic embryos, rather they were dependent on the medium components. In addition, in a previous study it has been found that glutamine (Gln) supplied in the medium was crucial for maintaining the primary amino acid (AA) metabolism and growth of the proliferating embryogenic cultures of Norway spruce (Carlsson et al., PLoS One 12(8):e0181785, 2017). Therefore, we hypothesised that Gln would be required as a significant source of N also during somatic embryo germination. By tracing the uptake of isotopically labelled N-sources from the medium and further into primary N assimilation, we found that Gln was the preferred source of N for the germinating somatic embryos, accounting for 50% of assimilated N. As the amounts of both arginine (Arg) and Gln were increased in the germinating somatic embryos, it also suggested that germination in low-intensity red light promoted N storage, similar to what has been observed in the zygotic embryo maturation in conifers (King, Gifford, Plant Physiol 113:1125-1135, 1997).
  •  
46.
  • Collins, Emily, et al. (författare)
  • Limited effective gene flow between two interfertile red oak species
  • 2015
  • Ingår i: Trees. - : Springer Science and Business Media LLC. - 0931-1890 .- 1432-2285. ; 29:4, s. 1135-1148
  • Tidskriftsartikel (refereegranskat)abstract
    • Highly elevated differentiation in different life stages between two interfertile oak species at a CONSTANS -like gene suggests a role of this gene in pre-zygotic isolation and adaptive divergence between species. Genome-wide differentiation patterns among oak species suggest that divergent selection can maintain species-specific adaptations and morphological integrity by reducing effective interspecific gene flow. While there is evidence for both pre- and post-zygotic isolation mechanisms in oaks (e.g., differences in flowering time, selection against hybrids), these mechanisms are rarely studied at each life stage from acorns to adult trees within the same forest. To assess the reproductive isolation mechanisms between two ecologically divergent species, we (1) quantified the number of hybrids in different life stages in Quercus rubra and Quercus ellipsoidalis, two interfertile red oaks with different adaptations to drought, and (2) assessed the timing of bud burst in both natural populations and in a seedling common garden trial. The low number of hybrids in all life stages suggested pre-zygotic isolation between species or selection in very early life stages that have not been sampled (e.g., early seed abortion). Significant differences in bud burst were found in two consecutive years between species in a common garden seedling trial but not in natural populations of the same provenance. In addition, we found evidence for divergent selection on several gene loci between species in each life stage. In particular, an SSR repeat located within the coding sequence of a CONSTANS-like gene, a locus involved in the photoperiodic regulation of flowering time and development, showed very high interspecific differentiation between species in all life stages (mean F (ST) = 0.83), compared to the average neutral differentiation of 3.7 %.
  •  
47.
  • DANELL, E (författare)
  • FORMATION AND GROWTH OF THE ECTOMYCORRHIZA OF CANTHARELLUS-CIBARIUS
  • 1994
  • Ingår i: MYCORRHIZA. - : SPRINGER VERLAG. - 0940-6360. ; 5:2, s. 89-97
  • Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
    • New data on the physiology of Cantharellus cibarius mycorrhiza formation has resulted in a new aseptic routine method for in vitro formation. The advances are short formation time, healthy plants and reliable colonization. A high glucose demand and a good
  •  
48.
  • Drobyshev, Igor (författare)
  • Root connections affect radial growth of balsam poplar trees
  • 2016
  • Ingår i: Trees - Structure and Function. - : Springer Science and Business Media LLC. - 0931-1890 .- 1432-2285. ; 30, s. 1775-1783
  • Tidskriftsartikel (refereegranskat)abstract
    • Connected root systems reduced the negative impact of August temperatures and insect outbreak on growth.Root connections between trees can be an ecological advantage of clonal plant species in environments with unevenly distributed resources. We investigated the effects of root connectivity in stands of balsam poplar in Quebec (Canada). We evaluated differences in growth response between groups of trees with and without root connections through climate-growth analyses, comparison of the growth dynamics, and analysis of growth response to a severe forest tent caterpillar (FTC) outbreak. Current May temperature had a positive influence on radial growth of both connected and non-connected trees. Growth of non-connected trees was negatively affected by August temperatures (r = -0.3) while connected trees did not reveal a significant relationship for that month. A mixed effect ANOVA showed a significant difference (F (1, 25) = 5.59, p = 0.02) in growth responses to FTC outbreak between connected and non-connected trees. Connected trees grew on average 16 % better than unconnected trees during the outbreak, with bootstrapped 95 % confidence range from 2.28 to 31.36 %. The study suggests a sharing of resources through root connections, affecting radial growth of connected balsam poplar trees under both average and extreme environmental conditions.
  •  
49.
  • Duethorn, Elisabeth, et al. (författare)
  • Influence of micro-site conditions on tree-ring climate signals and trends in central and northern Sweden
  • 2013
  • Ingår i: Trees. - : Springer Science and Business Media LLC. - 0931-1890 .- 1432-2285. ; 27:5, s. 1395-1404
  • Tidskriftsartikel (refereegranskat)abstract
    • Tree-ring chronologies are important indicators of pre-instrumental, natural climate variability. Some of the longest chronologies are from northern Fennoscandia, where ring width measurement series from living trees are combined with series from sub-fossil trees, preserved in shallow lakes, to form millennial-length records. We here assess the recent ends of such timeseries by comparing climate signals and growth characteristics in central and northern Sweden, of (1) trees growing at lakeshore micro-sites (representing the source of sub-fossil material of supra-long chronologies), with (2) trees collected in dryer micro-sites several meters inland. Calibration trials reveal a predominating June-September temperature signal in N-Sweden and a weaker but significant May-September precipitation signal in C-Sweden. At the micro-site level, the temperature signal in N-Sweden is stronger in the lakeshore trees compared to the inland trees, whereas the precipitation signal in C-Sweden remains unchanged among the lakeshore and inland trees. Tree-rings at cambial ages > 40 years are also substantially wider in the lakeshore micro-site in C-Sweden, and juvenile rings are more variable (and wider) in the dryer micro-site in N-Sweden (compared to the adjacent micro-sites). By combining the data of the various micro-sites with relict samples spanning the past 1,000 years, we demonstrate that growth rate differences at the micro-site scale can affect the low frequency trends of millennial-length chronologies. For the supra-long chronologies from northern Fennoscandia, that are derived from sub-fossil lake material, it is recommended to combine these data with measurement series from only lakeshore trees.
  •  
50.
  • Eklund, Leif, et al. (författare)
  • Enhanced growth and ethylene increases spiral grain formationin Picea abies and Abies balsamea trees
  • 2003
  • Ingår i: Trees. - : Springer. - 0931-1890 .- 1432-2285. ; 17:1, s. 81-86
  • Tidskriftsartikel (refereegranskat)abstract
    • Spiral grain angle in Norway spruce (Piceaabies) trees and balsam fir (Abies balsamea) seedlingswas investigated in relation to growth rate, endogenousand applied ethylene. Trees from stands of Norwayspruce, which were irrigated and fertilised in order to enhancegrowth, and trees having different growth rates innon-treated stands were studied. Stem growth rate at thestand level (m3 ha–1 year–1) was measured annually, orby means of microscopy on stem sections as the numberand size of tracheids produced. Enhanced growth increasedethylene evolution and maintained a high levelof left-handed spiral grain angle in comparison to slower-growing trees. An increased number of earlywoodtracheids in fast growing trees was correlated to a moreleft-handed spiral grain angle. Ethrel, applied to stems ofbalsam fir seedlings, increased the internal ethylene levelsin parallel with increased left-handed spiral grain angle.The results indicate that ethylene regulates the extentof spiral grain angle.
  •  
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