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1.	Reczey, K, et al. (författare) Cellulase production by T-reesei 1996 Ingår i: Bioresource Technology. - : Elsevier BV. - 1873-2976 .- 0960-8524. ; 57:1, s. 25-30 Tidskriftsartikel (refereegranskat)abstract Steam-pretreated willow has been found to be a suitable substrate for ethanol production in Sweden. The production of Trichoderma reesei cellulases on steam-pretreated willow (SPW) of varying cellulose content has been studied. Batch fermentations were performed in shake flasks, and in 4- and 22-l fermenters. SPW was found to be a suitable carbon source for cellulase production. At an initial solids concentration corresponding to 10 g/l cellulose, the yield was 108 filter paper units (FPU)/g cellulose after 3 days in the 22-l fermenter However, the yield was below 100 FPU/g cellulose in the shake flasks and 4-l fermenter The solid residue of enzymatically hydrolysed SPW was found to be nearly as good a substrate as SPW, although the cellulose content was as low as 20% in this hydrolysis residue. Using delignified SPW, the yield of cellulases was low, indicating that lignin does not inhibit cellulase production. By applying a pressure of 1.7 bar in the fermenter, the initial fermentation rate was increased. The productivity was increased from 12.2 FPU/l/h to 16.7 FPU/l/h, due to the higher level of dissolved oxygen at the higher pressure. Copyright (C) 1996 Elsevier Science Ltd.
2.	von Sivers, M, et al. (författare) Ethanol from lignocellulosics: A review of the economy 1996 Ingår i: Bioresource Technology. - : Elsevier BV. - 1873-2976 .- 0960-8524. ; 56:2-3, s. 131-140 Tidskriftsartikel (refereegranskat)abstract The present study is a review of published investigations regarding the economy of ethanol production from lignocellulosic material The objective is to present relations between and tendencies observed in different cost estimates. The influence of plant capacity and overall product yield on the ethanol production cost is investigated, as well as variations in capital costs in the different processes. The underlying technical and economic assumptions show a large variation between the various studies published. The variation in the ethanol production cost is large, from 18 to 151 US cent/l. The most important factor for the economic outcome is the overall ethanol yield. Other important parameters are the feedstock cost, which was found to vary between 22 and 61 US$/dry metric ton, and the plant capacity, which influences the capital cost. It is shown that there is a tendency towards a decrease in ethanol production cost with an increase in plant capacity for the enzymatic processes. A high yield also results in a decrease in production cost for the enzymatic and dilute acid processes in the papers reviewed. Copyright (C) 1996 Elsevier Science Ltd.
3.	Christakopoulos, Paul, et al. (författare) Direct conversion of sorghum carbohydrates to ethanol by a mixed microbial culture 1993 Ingår i: Bioresource Technology. - 0960-8524 .- 1873-2976. ; 45:2, s. 89-92 Tidskriftsartikel (refereegranskat)abstract The carbohydrates of sweet sorghum were directly converted to ethanol by a mixed culture of Fusarium oxysporum F3 and Saccharomyces cerevisiae 2541. A number of factors affecting this bioconversion was studied. Optimum ethanol yields of 33·2 g/100 g of total sorghum carbohydrates, corresponding to 10·3 g/100 g of fresh stalks, were obtained. These values represented 68·6% of the theoretical yield based on total polysaccharides and exceeded that based on oligosaccharides of sorghum by 53·7%. The results demonstrated that more than half of the sorghum polysaccharides were directly fermented to ethanol, thus making the process worthy of further investigation.
4.	Christakopoulos, Paul, et al. (författare) Direct ethanol conversion of pretreated straw by Fusarium oxysporum 1991 Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 35:3, s. 297-300 Tidskriftsartikel (refereegranskat)abstract Factors affecting the direct conversion of alkali pretreated straw to ethanol by Fusarium oxysporum F3 were investigated and the alkali level used for pretreatment and the degree of delignification of straw were found to be the most important. A linear correlation between ethanol yield and both the degree of straw delignification and the alkali level was observed. At optimum delignified straw concentration (4% w/v), a maximum ethanol yield of 0·275 g ethanol g−1 of straw was obtained corresponding to 67·8% of the theoretical yield.
5.	Christakopoulos, Paul, et al. (författare) Production and partial characterization of xylanase from Fusarium oxysporum 1996 Ingår i: Bioresource Technology. - 0960-8524 .- 1873-2976. ; 58:2, s. 115-119 Tidskriftsartikel (refereegranskat)abstract Production of xylanase by Fusarium oxysporum strain F3 was enhanced by optimization of initial pH of the culture medium, the type and concentration of nitrogen and carbon source, and the growth temperature. Under these conditions, yields as high as 245 U/ml of culture medium were obtained. The most important characteristic of the enzyme is its high pH stability. It retained 80 and 66% of the activity at pH 9.0 after 24 h at 4 and 30°C, respectively. Chromogenic (fluorogenic) 4-methylumbelliferyl-β-glycosides of xylose (MUX) and xylobiose (MUX2) were used to characterize xylanase multienzyme components, after separation by isoelectric focusing. The zymogram indicated one major, one minor xylanase and one active β-xylosidase exhibiting pI values of 9.5, 6.5 and 3.8, respectively
6.	Kalogeris, E, et al. (författare) Performance of an intermittent agitation rotating drum type bioreactor for solid-state fermentation of wheat straw 2003 Ingår i: Bioresource Technology. - 0960-8524 .- 1873-2976. ; 86:3, s. 207-213 Tidskriftsartikel (refereegranskat)abstract A laboratory bioreactor, designed for solid-state fermentation of thermophilic microorganisms, was operated for production of cellulases and hemicellulases by the thermophilic fungus Thermoascus aurantiacus. The suitability of the apparatus for the effective control of important operating variables affecting growth of microbes in solid-state cultivation was determined. Application of the optimum conditions found for the moisture content of the medium, growth temperature and airflow rate produced enzyme yields of 1709 U endoglucanase, 4 U cellobiohydrolase, 79 U β-glucosidase, 5.5 U FPA, 4490 U xylanase and 45 U β-xylosidase per g of dry wheat straw. The correlation between microorganism growth and production of enzymes was efficiently described by the Le Duy kinetic model. © 2002 Elsevier Science Ltd. All rights reserved.
7.	Rodhe, Lena (författare) Methods for determining the presence of slurry on the crop and in the upper soil layer after application to grassland 2003 Ingår i: Bioresource Technology. - 0960-8524 .- 1873-2976. ; 90:1, s. 81-88 Tidskriftsartikel (refereegranskat)abstract Slurry injection in grassland may lead to lower ammonia losses and enhance silage quality compared to surface spreading. In order to evaluate the performance of different slurry injectors, there is a need for methods which measure the degree of crop contamination and the position of the slurry in the soil after spreading. The aim of this study was to identify and test possible methods. The amount of cattle slurry present on the grass stubble after spreading was determined by measuring the electrical conductivity of water used for rinsing grass samples. The slurry placement in the soil profile was measured in cross-sections made through the slurry trails. Methods used were analyses of images taken by different cameras, resistance measurements and by measuring the dimensions of the slurry trails. The method for determining the presence of slurry on the crop was practically applicable and is considered to be reliable. The placement of slurry could not be sufficiently resolved from image taken by the different cameras or by measuring resistance. Instead, visual assessment and measurements of width and depth of the slurry trails gave a good description of the slurry placement in the soil profile. © 2003 Elsevier Science Ltd. All rights reserved.
8.	Sundh, I, et al. (författare) Effects of glucose overloading on microbial community structure and biogas production in a laboratory-scale anaerobic digester 2003 Ingår i: Bioresource Technology. - 0960-8524 .- 1873-2976. ; 89:3, s. 237-243 Tidskriftsartikel (refereegranskat)abstract This study characterizes the response of the microbial communities of a laboratory-scale mesophilic biogas process, fed with a synthetic substrate based on cellulose and egg albumin, to single pulses of glucose overloading (15 or 25 times the daily feed based on VS). The microbial biomass and community structure were determined from analyses of membrane phospholipids. The ratio between phospholipid fatty acids (PLFAs; eubacteria and eucaryotes) and di-ethers (PLEL; archaea) suggested that methanogens constituted 4-8% of the microbial biomass. The glucose addition resulted in transient increases in the total biomass of eubacteria while there were only small changes in community structure. The total gas production rate increased, while the relative methane content of the biogas and the alkalinity decreased. However, the biomass of methanogens was not affected by the glucose addition. The results show that the microbial communities of biogas processes can respond quickly to changes in the feeding rate. The glucose overload resulted in a transient general stimulation of degradation rates and almost a doubling of eubacterial biomass, although the biomass increase corresponded to only 7% of the glucose C added. (C) 2003 Elsevier Science Ltd. All rights reserved.
9.	Taherzadeh, Mohammad J, 1965-, et al. (författare) Conversion of dilute-acid hydrolyzates of spruce and birch to ethanol by fed-batch fermentation 1999 Ingår i: Bioresource Technology. - Exeter, United Kingdom : Elsevier Sci Ltd. - 0960-8524 .- 1873-2976. ; 69:1, s. 59-66 Tidskriftsartikel (refereegranskat)abstract Fermentation techniques for conversion of dilute acid hydrolyzates were examined. Batch and fed-batch fermentations of hydrolyzates from spruce and birch woods were made in a lab-scale (3.31) anaerobic bioreactor using the yeast Saccharomyces cerevisiae. The spruce and birch hydrolyzates contained high initial concentrations of furfural (2.2 and 5.7 g/l) and 5-hydroxymethylfurfural (HMF, 7.3 and 2.4 g/l), and were found to be strongly inhibiting to the yeast strain used in this study. Fermentation of the hydrolyzates was not possible using a batch mode of operation. However, using a fed-batch technique with a suitably adjusted feed rate, it was found possible to completely ferment the glucose and mannose sugars in both hydrolyzates. Most of the furfural (90%), and part of the HMF (40-70%), present in the hydrolyzates was converted during the fed-batch operation. It is suggested that the success of the fed-batch operation is related to the conversion of furfural and HMF.Fermentation techniques for conversion of dilute acid hydrolyzates were examined. Batch and fed-batch fermentations of hydrolyzates from spruce and birch woods were made in a lab-scale (3.31) anaerobic bioreactor using the yeast Saccharomyces cerevisiae. The spruce and birch hydrolyzates contained high initial concentrations of furfural (2.2 and 5.7 g/l) and 5-hydroxymethylfurfural (HMF, 7.3 and 2.4 g/l), and were found to be strongly inhibiting to the yeast strain used in this study. Fermentation of the hydrolyzates was not possible using a batch mode of operation. However, using a fed-batch technique with a suitably adjusted feed rate, it was found possible to completely ferment the glucose and mannose sugars in both hydrolyzates. Most of the furfural (90%), and part of the HMF (40-70%), present in the hydrolyzates was converted during the fed-batch operation. It is suggested that the success of the fed-batch operation is related to the conversion of furfural and HMF.
10.	Taherzadeh, Mohammad J, 1965-, et al. (författare) Production of mycelium biomass and ethanol from paper pulp sulfite liquor by Rhizopus oryzae 2003 Ingår i: Bioresource Technology. - : Elsevier Ltd. - 0960-8524 .- 1873-2976. ; 88:3, s. 167-177 Tidskriftsartikel (refereegranskat)abstract The cultivation conditions for Rhizopus oryzae grown in synthetic medium and paper pulp spent sulfite liquor (SSL) were investigated to achieve high biomass and ethanol yields using shake flasks and bioreactors. The fungus assimilated the hexoses glucose, mannose and galactose, and the pentoses xylose and arabinose as well as acetic acid which are present in SSL. The assimilation of hexoses was faster than pentoses during cultivation in a synthetic medium. However, all sugars were assimilated concomitantly during growth in SSL supplemented with ammonium, magnesium, calcium, phosphate, sulfate and trace amounts of some other metal ions (SSL-S). The medium composition had an important influence on biomass yield. The highest biomass yields, viz. 0.18 and 0.43 g biomass/g sugar were obtained, when the cells were cultivated in shake flasks with a synthetic medium containing glucose as carbon and energy source and SSL-S, respectively. The corresponding yields in a bioreactor with more efficient aeration were 0.22 and 0.55 g/g. In addition to the biomass, ethanol, lactic acid, and glycerol were important extracellular metabolites of the cultivation with maximum yields of 0.37, 0.30 and 0.09 g/g, respectively. When the source of sugars in the medium was exhausted, the fungus consumed the metabolites produced, such that the liquid medium was depleted of potential oxidizable nutrients. In general, there was a direct competition between lactic acid and ethanol among the metabolites. Poor medium compositions and cultivation conditions resulted in higher yields of lactic acid, whereas the ethanol and biomass yields were higher in rich media. SSL-S supported good growth of mycelium and a high ethanol yield.
11.	Adeboye, Peter, 1982, et al. (författare) A coniferyl aldehyde dehydrogenase gene from Pseudomonas sp. strain HR199 enhances the conversion of coniferyl aldehyde by Saccharomyces cerevisiae 2016 Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 212:July 2016, s. 11-19 Tidskriftsartikel (refereegranskat)abstract AbstractThe conversion of coniferyl aldehyde to cinnamic acids by Saccharomyces cerevisiae under aerobic growth conditions was previously observed. Bacteria such as Pseudomonas have been shown to harbor specialized enzymes for converting coniferyl aldehyde but no comparable enzymes have been identified in S. cerevisiae. CALDH from Pseudomonas was expressed in S. cerevisiae. An acetaldehyde dehydrogenase (Ald5) was also hypothesized to be actively involved in the conversion of coniferyl aldehyde under aerobic growth conditions in S. cerevisiae. In a second S. cerevisiae strain, the acetaldehyde dehydrogenase (ALD5) was deleted. A prototrophic control strain was also engineered. The engineered S. cerevisiae strains were cultivated in the presence of 1.1 mM coniferyl aldehyde under aerobic condition in bioreactors. The results confirmed that expression of CALDH increased endogenous conversion of coniferyl aldehyde in S. cerevisiae and ALD5 is actively involved with the conversion of coniferyl aldehyde in S. cerevisiae.
12.	Ahlgren, S., et al. (författare) Ammonium nitrate fertiliser production based on biomass : Environmental effects from a life cycle perspective 2008 Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 99:17, s. 8034-8041 Tidskriftsartikel (refereegranskat)abstract Ammonium nitrate and calcium ammonium nitrate are the most commonly used straight nitrogen fertilisers in Europe, accounting for 43% of the total nitrogen used for fertilisers. They are both produced in a similar way; carbonate can be added as a last step to produce calcium ammonium nitrate. The environmental impact, fossil energy input and land use from using gasified biomass (cereal straw and short rotation willow (Salix) coppice) as feedstock in ammonium nitrate production were studied in a cradle-to-gate evaluation using life cycle assessment methodology. The global warming potential in the biomass systems was only 22-30% of the impact from conventional production using natural gas. The eutrophication potential was higher for the biomass systems due to nutrient leaching during cultivation, while the acidification was about the same in all systems. The primary fossil energy use was calculated to be 1.45 and 1.37 MJ/kg nitrogen for Salix and straw, respectively, compared to 35.14 MJ for natural gas. The biomass production was assumed to be self-supporting with nutrients by returning part of the ammonium nitrate produced together with the ash from the gasification. For the production of nitrogen from Salix, it was calculated that 3914 kg of nitrogen can be produced every year from 1 ha, after that 1.6% of the produced nitrogen has been returned to the Salix production. From wheat straw, 1615 kg of nitrogen can be produced annually from 1 ha, after that 0.6% of the nitrogen has been returned. © 2008 Elsevier Ltd. All rights reserved.
13.	Ahlgren, Serina, et al. (författare) Nitrogen fertiliser production based on biogas - Energy input, environmental impact and land use 2010 Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 101, s. 7181-7184 Tidskriftsartikel (refereegranskat)abstract The aim of the present paper was to investigate the land use, environmental impact and fossil energy use when using biogas instead of natural gas in the production of nitrogen fertilisers The biogas was assumed to be produced from anaerobic digestion of ley grass and maize. The calculations showed that 1 ha of agricultural land in south-west Sweden can produce 1.7 metric ton of nitrogen in the form of ammonium nitrate per year from ley grass, or 3 6 ton from maize The impact on global warming, from cradle to gate, was calculated to be lower when producing nitrogen fertiliser from biomass compared with natural gas Eutrophication and acidification potential was higher in the biomass scenarios The greatest advantage of the biomass systems however lies in the potential to reduce agriculture's dependency on fossil fuels In the biomass scenarios, only 2-4 MJ of primary fossil energy was required, while 35 MJ/kg N was required when utilising natural gas (C) 2010 Elsevier Ltd All rights reserved
14.	Almeida, Joao, et al. (författare) Screening of Saccharomyces cerevisiae strains with respect to anaerobic growth in non-detoxified lignocellulose hydrolysate. 2009 Ingår i: Bioresource Technology. - : Elsevier BV. - 1873-2976 .- 0960-8524. ; 100, s. 3674-3677 Tidskriftsartikel (refereegranskat)abstract A microplate screening method was used to assess anaerobic growth of 12 Saccharomyces cerevisiae strains in barley straw, spruce and wheat straw hydrolysate. The assay demonstrated significant differences in inhibitor tolerance among the strains. In addition, growth inhibition by the three hydrolysates differed so that wheat hydrolysate supported growth up to 70%, while barley hydrolysate only supported growth up to 50%, with dilute-acid spruce hydrolysate taking an intermediate position.
15.	Alriksson, Björn, et al. (författare) Improving the fermentability of enzymatic hydrolysates of lignocellulose through chemical in-situ detoxification with reducing agents 2011 Ingår i: Bioresource Technology. - : Elsevier Ltd. - 0960-8524 .- 1873-2976. ; 102:2, s. 1254-1263 Tidskriftsartikel (refereegranskat)abstract Inhibitory lignocellulose hydrolysates were treated with the reducing agents dithionite and sulfite to achieve improved fermentability. Addition of these reducing agents (in the concentration range 5.0-17.5mM) to enzymatic hydrolysates of spruce wood or sugarcane bagasse improved processes based on both SHF (simultaneous hydrolysis and fermentation) and SSF (simultaneous saccharification and fermentation). The approach was exemplified in ethanolic fermentations with Saccharomyces cerevisiae and by using hydrolysates with sugar concentrations >100g/L (for SHF) and with 10% dry-matter content (for SSF). In the SHF experiments, treatments with dithionite raised the ethanol productivities of the spruce hydrolysate from 0.2 to 2.5g×L(-1)×h(-1) and of the bagasse hydrolysate from 0.9 to 3.9g×L(-1)×h(-1), values even higher than those of fermentations with reference sugar solutions without inhibitors. Benefits of the approach include that the addition of the reducing agent can be made in-situ directly in the fermentation vessel, that the treatment can be performed at a temperature and pH suitable for fermentation, and that the treatment results in dramatically improved fermentability without degradation of fermentable sugars. The many benefits and the simplicity of the approach offer a new way to achieve more efficient manufacture of fermentation products from lignocellulose hydrolysates.
16.	Alvarez, Rene, et al. (författare) The effect of temperature variation on biomethanation at high altitude. 2008 Ingår i: Bioresource Technology. - : Elsevier BV. - 1873-2976 .- 0960-8524. ; 99, s. 7278-7284 Tidskriftsartikel (refereegranskat)abstract The aim of the current study was to examine effects of daily temperature variations on the performance of anaerobic digestion. Forced square-wave temperature variations (between 11 and 25, 15 and 28, and 19 and 32 degrees C) were imposed on a bench-scale digester using a mixture of llama-cow-sheep manure in a semi-continuous process. The volumetric biogas production rate, methane yield, and the volatile solid reductions were compared with the results obtained from anaerobic digestion (AD) at constant temperatures. The forced cyclic variations of temperature caused large cyclic variations in the rate of gas production and the methane content. As much as 94-97% of the daily biogas was obtained in the 12h half-cycle at high temperature. The values for volumetric biogas production rate and methane yield increased at higher temperatures. The average volumetric biogas production rate for cyclic operation between 11 and 25 degrees C was 0.22Ld(-1)L(-1) with a yield of 0.07m(3)CH(4)kg(-1) VS added (VSadd), whereas for operation between 15 and 29 degrees C the volumetric biogas production rate increased by 25% (to 0.27Ld(-1)L(-1) with a yield of 0.08m(3)CH(4)kg(-1) VSadd). In the highest temperature region a further increase of 7% in biogas production was found and the methane yield was 0.089m(3)CH(4)kg(-1) VSadd. The employed digester showed an immediate response when the temperature was elevated, which indicates a well-maintained metabolic capacity of the methanogenic bacteria during the period of low temperature. Overall, periodic temperature variations appear to give less decrease in process performance than a priori anticipated.
17.	Atasoy, Merve, et al. (författare) A comprehensive study of volatile fatty acids production from batch reactor to anaerobic sequencing batch reactor by using cheese processing wastewater 2020 Ingår i: Bioresource Technology. - : Elsevier. - 0960-8524 .- 1873-2976. ; 311 Tidskriftsartikel (refereegranskat)abstract Volatile fatty acids (VFAs) has great potential for closed-loop production in dairy industries via resource recovery from waste-streams. In the current study, the transition of VFA production from batch reactor to anaerobic sequencing batch reactor (ASBR) by using cheese industry wastewater under alkali pH was evaluated with respect to seed sludge structure, microbial diversity and reactor type. The transition from the batch reactor to the ASBR demonstrated that the maximum VFA production yield (g COD/g SCOD) was comparable in two reactors (batch: 0.97; ASBR: 0.94), whereas, the dominant acid type was different (batch: 49% lactic acid; ASBR: 80% propionic acid). There was a significant correlation between the productions of butyric acid with Gracilibacteraceae and Desulfovibrionaceae; propionic acid with Desulfovibrionaceae and Synergistaceae; lactic acid with Pseudomonadaceae and Rhodocyclaceae. The high VFA production efficiency can be achieved by long term reactor operation, which enables the shift from industrial waste-streams to biorefineries.
18.	Atasoy, Merve, et al. (författare) Bio-based volatile fatty acid production and recovery from waste streams: Current status and future challenges 2018 Ingår i: Bioresource Technology. - : Elsevier. - 0960-8524 .- 1873-2976. ; 268, s. 773-786 Forskningsöversikt (refereegranskat)abstract Bio-based volatile fatty acid (VFA) production from waste-stream is getting attention due to increasing market demand and wide range usage area as well as its cost-effective and environmentally friendly approach. The aim of this paper is to give a comprehensive review of bio-based VFA production and recovery methods and to give an opinion on future research outlook. Effects of operation conditions including pH, temperature, retention time, type of substrate and mixed microbial cultures on VFA production and composition were reviewed. The recovery methods in terms of gas stripping with absorption, adsorption, solvent extraction, electrodialysis, reverse osmosis, nanofiltration, and membrane contractor of VFA were evaluated. Furthermore, strategies to enhance bio-based VFA production and recovery from waste streams, specifically, in-line VFA recovery and bioaugmentation, which are currently not used in common practice, are seen as some of the approaches to enhance bio-based VFA production.
19.	Atasoy, Merve, et al. (författare) Volatile fatty acid production from semi-synthetic milk processing wastewater under alkali pH : The pearls and pitfalls of microbial culture 2020 Ingår i: Bioresource Technology. - : Elsevier. - 0960-8524 .- 1873-2976. ; 297 Tidskriftsartikel (refereegranskat)abstract Volatile fatty acids (WA) are one of the most promising sustainable and environmentally friendly bioproduct owing to their wide usage area and high market demand. For this reason, in this study, the evaluation of VFA production from pure and mixed bacterial cultures was aimed. Three different mixed cultures with C. aceticum, C. butyricum and P. acidipropionici as pure cultures were used for inoculation of milk processing wastewater fermentation under pH 10 for 15 days. The mixed culture fermentation had the highest VFA production efficiency whereas the highest amount of acetic, butyric and propionic acid productions were obtained by C. aceticum, C. butyricum and P. acidipropionici, respectively. Also, the mixed cultures demonstrated faster pH regulation and acclimation than the pure cultures tested. Therefore, development of synthetic cultures may offer a useful approach to produce VFA mixtures with one-dominant acid type and with high production efficiency.
20.	Atasoy, Merve, et al. (författare) Volatile fatty acids production via mixed culture fermentation : Revealing the link between pH, inoculum type and bacterial composition 2019 Ingår i: Bioresource Technology. - : ELSEVIER SCI LTD. - 0960-8524 .- 1873-2976. ; 292 Tidskriftsartikel (refereegranskat)abstract The aim of the study was to investigate the effects of operational parameters, inoculum type and bacterial community on mixed culture fermentation to produce one dominant acid type in the mixture of volatile fatty acids (VFA). The study was performed using three different inocula (large&small granular and slurry) with glucose under various initial pH. The VFA production efficiency reached to 0,97 (gCOD/gSCOD) by granular sludge. VFA composition was changed by initial pH: in neutral conditions, acetic acid; in acidic conditions, acetic and butyric acids, in alkali conditions butyric acid were dominated, respectively. The VFA production was positively affected by the high relative abundance of Firmicutes. On the contrary, a negative correlation was seen between VFA production and the relative abundance of Chloroflexi. The results revealed the physical sludge structure of inoculum was the key factor for production efficiency, whereas, pH was the most important parameter to affect VFA composition.
21.	Awasthi, Mukesh Kumar, et al. (författare) Advanced approaches for resource recovery from wastewater and activated sludge: A review 2023 Ingår i: Bioresource Technology. - 0960-8524 .- 1873-2976. ; 384 Forskningsöversikt (refereegranskat)abstract Due to resource scarcity, current industrial systems are switching from waste treatment, such as wastewater treatment and biomass, to resource recovery (RR). Biofuels, manure, pesticides, organic acids, and other bioproducts with a great market value can be produced from wastewater and activated sludge (AS). This will not only help in the transition from a linear economy to a circular economy, but also contribute to sustainable development. However, the cost of recovering resources from wastewater and AS to produce value-added products is quite high as compared to conventional treatment methods. In addition, most antioxidant technologies remain at the laboratory scale that have not yet reached the level at industrial scale. In order to promote the innovation of resource recovery technology, the various methods of treating wastewater and AS to produce biofuels, nutrients and energy are reviewed, including biochemistry, thermochemistry and chemical stabilization. The limitations of wastewater and AS treatment methods are prospected from biochemical characteristics, economic and environmental factors. The biofuels derived from third generation feedstocks, such as wastewater are more sustainable. Microalgal biomass are being used to produce biodiesel, bioethanol, biohydrogen, biogas, biooils, bioplastics, biofertilizers, biochar and biopesticides. New technologies and policies can promote a circular economy based on biological materials.
22.	Azeem, Muhammad, et al. (författare) Sustainable bio-production of styrene from forest waste 2013 Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 144, s. 684-688 Tidskriftsartikel (refereegranskat)abstract A strain of Penicillium expansum was studied for the production of styrene using forest waste biomass as a feeding substrate. The fungal strain was cultivated on bark of various trees supplemented with yeast extract and the volatiles produced were collected on Tenax TA and analyzed by gas chromatography-mass spectrometry. Fungus cultured on grated soft bark of pine (Pinus sylvestris) stems (GPB) and mature bark of oak (Quercus robur) supplemented with yeast extract produced relatively the highest amounts of styrene. The maximum styrene production rate was 52.5 mu g/h, 41 mu g/h and 27 mu g/h from fungus cultivated on 50 mL liquid media with 10 g GPB or mature bark of oak and potato dextrose broth respectively. These promising results suggest that the fungal strain could be used to produce "green" styrene plastics using renewable forest waste biomass.
23.	Badshah, Malik, et al. (författare) Anaerobic treatment of methanol condensate from pulp mill compared with anaerobic treatment of methanol using mesophilic UASB reactors. 2012 Ingår i: Bioresource Technology. - : Elsevier BV. - 1873-2976 .- 0960-8524. ; 125C, s. 318-327 Tidskriftsartikel (refereegranskat)abstract The feasibility of anaerobic treatment of methanol condensate from pulp and paper mill in UASB reactor was investigated and compared with the anaerobic treatment of methanol. The UASB reactor treating methanol condensate was operated for 480days with minimum problems of overload. COD removal from methanol condensate and methanol under normal operating conditions ranged from 84-86% to 86-98%, respectively. Under optimal conditions (OLR=5.0gCODL(-1)day(-1), COD(influent)=11.40gL(-1)) a methane yield of 0.29 NL CH(4) per g COD(removed) (at standard temperature and pressure) was achieved during the treatment of methanol condensate. The recovery time of the microorganisms after several overloads was decreasing each time probably due to the adaptation to methanol condensate. These findings indicate that methanol condensate can be efficiently treated in a UASB reactor with the benefit of biogas production. As a bonus effect of the treatment, much of the smell of the feed was eliminated.
24.	Badshah, Malik, et al. (författare) Use of an Automatic Methane Potential Test System for evaluating the biomethane potential of sugarcane bagasse after different treatments. 2012 Ingår i: Bioresource Technology. - : Elsevier BV. - 1873-2976 .- 0960-8524. ; 114, s. 262-269 Tidskriftsartikel (refereegranskat)abstract A multi-channel analyzer was used to evaluate biogas potential of sugarcane bagasse (SCB). The Automatic Methane Potential Test System contained fifteen parallel reactors and the same number of gas flow meters attached to the acquisition system. The set of reactors - gas flow meters gave reproducible results during anaerobic digestion of chemically defined carbon source and the units were used to evaluate the biomethane potential of SCB after different pretreatments, such as treatment with water, acid, acid followed by enzymatic treatment and acid followed by treatment with inactive enzymes. Combined pretreatment with 2% sulphuric acid and enzymatic hydrolysis (3.5% enzymes) resulted in conversion of 79% to monomeric sugars present in SCB. SCB treated with acid followed by enzymatic hydrolysis achieved the methane yield of 200NL per kg VS(added). Enzymatic saccharification of acid pretreated SCB resulted in increase of methane yield by 16±5% compared to that from acid treated SCB.
25.	Bajracharya, Suman, et al. (författare) Advances in cathode designs and reactor configurations of microbial electrosynthesis systems to facilitate gas electro-fermentation 2022 Ingår i: Bioresource Technology. - : Elsevier. - 0960-8524 .- 1873-2976. ; 354 Tidskriftsartikel (refereegranskat)abstract In gas fermentation, a range of chemolithoautotrophs fix single-carbon (C1) gases (CO2 and CO) when H2 or other reductants are available. Microbial electrosynthesis (MES) enables CO2 reduction by generating H2 or reducing equivalents with the sole input of renewable electricity. A combined approach as gas electro-fermentation is attractive for the sustainable production of biofuels and biochemicals utilizing C1 gases. Various platform compounds such as acetate, butyrate, caproate, ethanol, butanol and bioplastics can be produced. However, technological challenges pertaining to the microbe-material interactions such as poor gas-liquid mass transfer, low biomass and biofilm coverage on cathode, low productivities still exist. We are presenting a review on latest developments in MES focusing on the configuration and design of cathodes that can address the challenges and support the gas electro-fermentation. Overall, the opportunities for advancing CO and CO2-based biochemicals and biofuels production in MES with suitable cathode/reactor design are prospected.
26.	Bécsy-Jakab, Villő Enikő, et al. (författare) Extraction, recovery, and characterization of lignin from industrial corn stover lignin cake 2024 Ingår i: Bioresource Technology. - : Elsevier Ltd. - 0960-8524 .- 1873-2976. ; 399 Tidskriftsartikel (refereegranskat)
27.	Bengtsson, Simon, et al. (författare) Production of polyhydroxyalkanoates by activated sludge treating a paper mill wastewater 2008 Ingår i: Bioresource Technology. - : Elsevier BV. - 1873-2976 .- 0960-8524. ; 99:3, s. 509-516 Tidskriftsartikel (refereegranskat)abstract Production of polyhydroxyalkanoates (PHAs) in activated sludge treating wastewater represents an economical and environmental promising alternative to pure culture fermentations. A process for production of PHA from a paper mill wastewater was examined at laboratory scale. The three stage process examined consisted of acidogenic fermentation to convert wastewater organic matter to volatile fatty acids (VFAs), an activated sludge system operating under feast/famine conditions to enrich for PHA producing organisms and accumulation of PHA in batch experiments. After fermentation of the wastewater, 74% of the soluble COD was present as VFA (acetate, propionate, butyrate and valerate) and the resulting PHA after batch accumulation consisted of 31-47 mol% hydroxybutyrate and 5369 mol% hydroxyvalerate. The maximum PHA content achieved was 48% of the sludge dry weight and the three stage process exhibited a potential to produce 0.11 kg of PHA per kg of influent COD treated. (c) 2007 Elsevier Ltd. All rights reserved.
28.	Bhaskar, Thallada, et al. (författare) New Horizons in Biotechnology - NHBT 2015 2016 Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 213 Tidskriftsartikel (refereegranskat)
29.	Blomqvist, Johanna, et al. (författare) The effect of a combined biological and thermo-mechanical pretreatment of wheat straw on energy yields in coupled ethanol and methane generation 2015 Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 194, s. 7-13 Tidskriftsartikel (refereegranskat)abstract Ethanol and biogas are energy carriers that could contribute to a future energy system independent of fossil fuels. Straw is a favorable bioenergy substrate as it does not compete with food or feed production. As straw is very resistant to microbial degradation, it requires a pretreatment to insure efficient conversion to ethanol and/or methane. This study investigates the effect of combining biological pretreatment and steam explosion on ethanol and methane yields in order to improve the coupled generation process. Results show that the temperature of the steam explosion pretreatment has a particularly strong effect on possible ethanol yields, whereas combination with the biological pretreatment showed no difference in overall energy yield. The highest overall energy output was found to be 10.86 MJ kg VS-1 using a combined biological and steam explosion pretreatment at a temperature of 200 degrees C. (C) 2015 Elsevier Ltd. All rights reserved.
30.	Brancoli, Pedro, 1989-, et al. (författare) Changes in carbon footprint when integrating production of filamentous fungi in 1st generation ethanol plants 2017 Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. Tidskriftsartikel (refereegranskat)abstract Integrating the cultivation of edible filamentous fungi in the thin stillage from ethanol production is presently being considered. This integration can increase the ethanol yield while simultaneously producing a new value-added protein-rich biomass that can be used for animal feed. This study uses life cycle assessment to determine the change in greenhouse gas (GHG) emissions when integrating the cultivation of filamentous fungi in ethanol production. The result shows that the integration performs better than the current scenario when the fungal biomass is used as cattle feed for system expansion and when energy allocation is used. It performs worse if the biomass is used as fish feed. Hence, integrating the cultivation of filamentous fungi in 1st generation ethanol plants combined with proper use of the fungi can lead to a reduction of GHG emissions which, considering the number of existing ethanol plants, can have a significant global impact.
31.	Bredberg, Katarina, et al. (författare) Reduction of vanadium(V) with Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans 2004 Ingår i: Bioresource Technology. - : Elsevier BV. - 1873-2976 .- 0960-8524. ; 92:1, s. 93-96 Tidskriftsartikel (refereegranskat)abstract Biotechnological leaching has been proposed as a suitable method for extraction of vanadium from spent catalysts and oil ash. In the biological leaching process, the vanadium(V) can be reduced to vanadium(IV), which is a less toxic and more soluble form of the vanadium. The present investigation showed that Acidithiobacillus ferrooxidans efficiently reduced vanadium(V) in the form of vanadium pentaoxide, to vanadyl(IV) ions, and tolerated high concentrations of vanadium(IV) and vanadium(V). A. ferrooxidans was compared with Acidithiobacillus thiooxidans, which has previously been utilized for vanadium leaching and reduction. Vanadium pentaoxide and sodium vanadate were used as model compounds. The results of this study indicate possibilities to develop an economical and technically feasible process for biotechnological vanadium recovery. (C) 2003 Elsevier Ltd. All rights reserved.
32.	Brinza, Loredana, et al. (författare) Cadmium tolerance and adsorption by the marine brown alga Fucus vesiculosus from the Irish Sea and the Bothnian Sea 2009 Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 100:5, s. 1727-1733 Tidskriftsartikel (refereegranskat)abstract Cadmium (Cd) uptake capacities and Cd tolerance of the marine alga Fucus vesiculosus from the Irish Sea (salinity 35 psu) and from the Bothnian Sea (northern Baltic, 5 psu) were quantified. These data were complemented by measurements of changes in maximal photosynthetic rate (Pmax), dark respiration rate and variable fluorescence vs. maximal fluorescence (Fv:Fm). At concentrations between 0.01 and 1 mmol Cd l−1, F. vesiculosus from the Bothnian Sea adsorbed significantly more (about 98%) Cd compared with F. vesiculosus from the Irish Sea. The photosynthetic measurements showed that the Bothnian Sea F. vesiculosus were more sensitive to Cd exposure than the Irish Sea algae. The algae from the Irish Sea showed negative photosynthetic effects only at 1 mmol Cd l−1, which was expressed as a decreased Pmax (−12.3%) and Fv:Fm (−4.6%). On the contrary, the algae from the Bothnian Sea were negatively affected already at Cd concentrations as low at 0.1 mmol Cd l−1. They exhibited increased dark respiration (+11.1%) and decreased Fv:Fm (−13.9%). The results show that F. vesiculosus from the Bothnian Sea may be an efficient sorption substrate for Cd removal from Cd contaminated seawater and this algae type may also have applications for wastewater treatment.
33.	Cadena, Edith M., et al. (författare) On hexenuronic acid (HexA) removal and mediator coupling to pulp fiber in the laccase/mediator treatment 2011 Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 102:4, s. 3911-3917 Tidskriftsartikel (refereegranskat)abstract Flax soda/AQ pulps were treated with different fungal laccase-mediator combinations followed by physical and chemical characterization of the pulps to obtain a thorough understanding of the laccase/mediator effects on hexenuronic acid (HexA) removal and the coupling of mediator onto pulps for fiber functionalization. Large differences were found and the presence of lauryl gallate (LG) during Trametes villosa laccase (TvL) treatment (TvL + LG) resulted in a much larger reduction of pulp-linked HexA than the combination of p-coumaric acid (PCA) and Pycnoporus cinnabarinus laccase (PcL). A major portion of LG became attached to the pulp as revealed by an increase in the kappa number and further confirmed by thioacidolysis and H-1 NMR analysis of solubilized pulp fractions. Additional experiments with other chemical pulps and isolated pulp xylan and lignin revealed that HexA seems to be the sole pulp component attacked by TvL + LG. As a substrate for TvL, the reaction preference order is PCA > HexA > LG.
34.	Carlos, Martin, et al. (författare) Adaptation of a recombinant xylose-utilizing Saccharomyces cerevisiae strain to a sugarcane bagasse hydrolysate with high content of fermentation inhibitors 2007 Ingår i: Bioresource Technology. - : Elsevier BV. - 1873-2976 .- 0960-8524. ; 98:9, s. 1767-1773 Tidskriftsartikel (refereegranskat)abstract Adaptation of a xylose-utilizing genetically engineered strain of Saccharomyces cerevisiae to sugarcane bagasse hydrolysates by cultivation during 353 h using medium with increasing concentrations of inhibitors, including phenolic compounds, furaldehydes and aliphatic acids, led to improved performance with respect to ethanol production. The remaining xylose concentration in the medium at the end of the cultivation was 5.2 g l(-1), while it was 11 g l(-1) in the feed, indicating that approximately half of the xylose was consumed. The performance of the adapted strain was compared with the parental strain with respect to its ability to ferment three bagasse hydrolysates with different inhibitor concentration. The ethanol yield after 24 h of fermentation of the bagasse hydrolysate with lowest inhibitor concentration increased from 0.18 g g(-1) of total sugar with the non-adapted strain to 0.38 g g(-1) with the adapted strain. The specific ethanol productivity increased from 1.15 g ethanol per g initial biomass per h with the non-adapted strain to 2.55 g g(-1) h(-1) with the adapted strain. The adapted strain performed better than the non-adapted also in the two bagasse hydrolysates containing higher concentrations of inhibitors. The adapted strain converted the inhibitory furaldehydes 2-furaldehyde (furfural) and 5-hydroxymethyl-2-furaldehyde (HMF) at a faster rate than the non-adapted strain. The xylose-utilizing ability of the yeast strain did not seem to be affected by the adaptation and the results suggest that ethanol rather than xylitol was formed from the consumed xylose. (c) 2006 Elsevier Ltd. All rights reserved.
35.	Carvalho, Lara, et al. (författare) Techno-economic assessment of catalytic gasification of biomass powders for methanol production 2017 Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 237, s. 167-177 Tidskriftsartikel (refereegranskat)abstract This study evaluated the techno-economic performance and potential benefits of methanol production through catalytic gasification of forest residues and lignin. The results showed that while catalytic gasification enables increased cold gas efficiencies and methanol yields compared to non-catalytic gasification, the additional pre-treatment energy and loss of electricity production result in small or no system efficiency improvements. The resulting required methanol selling prices (90â€“130Â â‚¬/MWh) are comparable with production costs for other biofuels. It is concluded that catalytic gasification of forest residues can be an attractive option as it provides operational advantages at production costs comparable to non-catalytic gasification. The addition of lignin would require lignin costs below 25Â â‚¬/MWh to be economically beneficial.
36.	Cavka, Adnan, et al. (författare) Detoxification of lignocellulosic hydrolysates using sodium borohydride 2013 Ingår i: Bioresource Technology. - : Elsevier. - 0960-8524 .- 1873-2976. ; 136, s. 368-376 Tidskriftsartikel (refereegranskat)abstract Addition of sodium borohydride to a lignocellulose hydrolysate of Norway spruce affected the fermentability when cellulosic ethanol was produced using Saccharomyces cerevisiae. Treatment of the hydrolysate with borohydride improved the ethanol yield on consumed sugar from 0.09 to 0.31 g/g, the balanced ethanol yield from 0.02 to 0.30 g/g, and the ethanol productivity from 0.05 to 0.57 g/(L×h). Treatment of a sugarcane bagasse hydrolysate gave similar results, and the experiments indicate that sodium borohydride is suitable for chemical in-situ detoxification. The model inhibitors coniferyl aldehyde, p-benzoquinone, 2,6-dimethoxybenzoquinone, and furfural were efficiently reduced by treatment with sodium borohydride, even under mild reaction conditions (20°C and pH 6.0). While addition of sodium dithionite to pretreatment liquid from spruce improved enzymatic hydrolysis of cellulose, addition of sodium borohydride did not. This result indicates that the strong hydrophilicity resulting from sulfonation of inhibitors by dithionite treatment was particularly important for alleviating enzyme inhibition.
37.	Cavka, Adnan, et al. (författare) Techno-economic evaluation of conditioning with sodium sulfite for bioethanol production from softwood 2015 Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 196, s. 129-135 Tidskriftsartikel (refereegranskat)abstract Conditioning with reducing agents allows alleviation of inhibition of biocatalytic processes by toxic by-products generated during biomass pretreatment, without necessitating the introduction of a separate process step. In this work, conditioning of steam-pretreated spruce with sodium sulfite made it possible to lower the yeast and enzyme dosages in simultaneous saccharification and fermentation (SSF) to 1 g/L and 5 FPU/g WIS, respectively. Techno-economic evaluation indicates that the cost of sodium sulfite can be offset by benefits resulting from a reduction of either the yeast load by 0.68 g/L or the enzyme load by 1 FPU/g WIS. As those thresholds were surpassed, inclusion of conditioning can be justified. Another potential benefit results from shortening the SSF time, which would allow reducing the bioreactor volume and result in capital savings. Sodium sulfite conditioning emerges as an opportunity to lower the financial uncertainty and compensate the overall investment risk for commercializing a softwood-to-ethanol process. (C) 2015 The Authors. Published by Elsevier Ltd.
38.	Chandolias, Konstantinos, 1985-, et al. (författare) Biohydrogen and carboxylic acids production from wheat straw hydrolysate 2016 Ingår i: Bioresource Technology. - Elsevier : Elsevier BV. - 0960-8524 .- 1873-2976. Tidskriftsartikel (refereegranskat)
39.	Chang, Jo-Shu, et al. (författare) Recent advances in lignocellulosic biomass refinery 2022 Ingår i: Bioresource Technology. - : Elsevier. - 0960-8524 .- 1873-2976. ; 347 Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
40.	Chen, Feng, et al. (författare) Shiitake cultivation as biological preprocessing of lignocellulosic feedstocks – Substrate changes in crystallinity, syringyl/guaiacyl lignin and degradation-derived by-products 2022 Ingår i: Bioresource Technology. - : Elsevier. - 0960-8524 .- 1873-2976. ; 344:Part B Tidskriftsartikel (refereegranskat)abstract Formulation of substrates based on three hardwood species combined with modulation of nitrogen content by whey addition (0–2%) was investigated in an experiment designed in D-optimal model for their effects on biological preproceesing of lignocellulosic feedstock by shiitake mushroom (Lentinula edodes) cultivation. Nitrogen loading was shown a more significant role than wood species for both mushroom production and lignocellulose degradation. The fastest mycelial colonisation occurred with no nitrogen supplementation, but the highest mushroom yields were achieved when 1% whey was added. Low nitrogen content resulted in increased delignification and minimal glucan consumption. Delignification was correlated with degradation of syringyl lignin unit, as indicated by a significant reduction (41.5%) of the syringyl-to-guaiacyl ratio after cultivation. No significant changes in substrate crystallinity were observed. The formation of furan aldehydes and aliphatic acids was negligible during the pasteurisation and fungal cultivation, while the content of soluble phenolics increased up to seven-fold.
41.	Chen, Feng, et al. (författare) Spent mushroom substrates for ethanol production – Effect of chemical and structural factors on enzymatic saccharification and ethanolic fermentation of Lentinula edodes-pretreated hardwood 2022 Ingår i: Bioresource Technology. - : Elsevier. - 0960-8524 .- 1873-2976. ; 347 Tidskriftsartikel (refereegranskat)abstract Spent mushroom substrates (SMS) from cultivation of shiitake (Lentinula edodes) on three hardwood species were investigated regarding their potential for cellulose saccharification and for ethanolic fermentation of the produced hydrolysates. High glucan digestibility was achieved during enzymatic saccharification of the SMSs, which was related to the low mass fractions of lignin and xylan, and it was neither affected by the relative content of lignin guaiacyl units nor the substrate crystallinity. The high nitrogen content in SMS hydrolysates, which was a consequence of the fungal pretreatment, was positive for the fermentation, and it ensured ethanol yields corresponding to 84–87% of the theoretical value in fermentations without nutrient supplementation. Phenolic compounds and acetic acid were detected in the SMS hydrolysates, but due to their low concentrations, the inhibitory effect was limited. The solid leftovers resulting from SMS hydrolysis and the fermentation residues were quantified and characterized for further valorisation.
42.	Chen, Xiaowen, et al. (författare) Kinetics and mechanism of autohydrolysis of hardwoods 2010 Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 101:20, s. 7812-7819 Tidskriftsartikel (refereegranskat)abstract Autohydrolysis using water is a promising method to extract hemicelluloses from wood prior to pulping in order to make co-products such as ethanol and acetic acid besides pulp. Many studies have been carried out on the kinetics and mechanism of autohydrolysis using batch reactors. The present study was performed in a continuous mixed flow reactor where the wood chips are retained in a basket inside the reactor. This reactor is well suited to determine intrinsic kinetics of hemicellulose dissolution because the dissolved products are rapidly removed from the reactor, thus minimizing further hydrolysis and degradation of the hemicelluloses in solution. The xylan removal rate follows an S-shaped behavior. GPC analysis of the continuously removed extract shows that the dissolved xylan oligomers have a DP smaller than about 25. Lignin-free xylan oligomers and cellulose oligomers are the major components dissolved in the initial stage of autohydrolysis, while xylan covalently bound to lignin (i.e. an LCC) is the major component removed during the later stage of autohydrolysis. The molecular weight of the dissolved components decreases with time in the second stage. The kinetics of xylan removal are explained in terms of a mechanism based on recent knowledge of the ultrastructure of the cell fibre wall.
43.	Chong, Sun-Li, 1976, et al. (författare) Immobilization of bacterial feruloyl esterase on mesoporous silica particles and enhancement of synthetic activity by hydrophobic-modified surface 2019 Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 293 Tidskriftsartikel (refereegranskat)abstract Here, we demonstrated the immobilization of bacterial feruloyl esterase (FAE) from Butyrivibrio sp. XPD2006, Lactobacillus crispatus, Butyrivibrio sp. AE2015, Ruminococcus albus, Cellulosilyticum ruminicola and Clostridium cellulovorans on SBA-15 and their ability to synthesize butyl ferulate (BFA). The BFae2 from Butyrivibrio sp. XPD2006 showed the best catalytic efficiency. High BFA yield was produced when the immobilization of BFae2 took place with a high protein loading and narrow pore sized SBA-15, suggesting alteration of enzyme behavior due to the crowding environment in SBA-15. Grafting of SBA-15 with octyl moieties led to shrinking pore size and resulted in 2.5-fold increment of BFA activity compared to the free enzyme and 70%mol BFA was achieved. The BFae2 encapsulated in hydrophobic-modified SBA-15 endured up to seven reaction cycles while the BFA activity remained above 60%. This is the first report showing the superior performance of hydrophobic-modified surface to entrap FAE to produce fatty phenolic esters.
44.	Corbin, Kendall R., et al. (författare) Grape marc as a source of carbohydrates for bioethanol : Chemical composition, pre-treatment and saccharification. 2015 Ingår i: Bioresource Technology. - : Elsevier. - 0960-8524 .- 1873-2976. ; 193, s. 76-83 Tidskriftsartikel (refereegranskat)abstract Global grape production could generate up to 13 Mt/yr of wasted biomass. The compositions of Cabernet Sauvignon (red marc) and Sauvignon Blanc (white marc) were analyzed with a view to using marc as raw material for biofuel production. On a dry weight basis, 31-54% w/w of the grape marc consisted of carbohydrate, of which 47-80% was soluble in aqueous media. Ethanol insoluble residues consisted mainly of polyphenols, pectic polysaccharides, heteroxylans and cellulose. Acid and thermal pre-treatments were investigated for their effects on subsequent cellulose saccharification. A 0.5M sulfuric acid pre-treatment yielded a 10% increase in the amount of liberated glucose after enzymatic saccharification. The theoretical amount of bioethanol that could be produced by fermentation of grape marc was up to 400 L/t. However, bioethanol from only soluble carbohydrates could yield 270 L/t, leaving a polyphenol enriched fraction that may be used in animal feed or as fertilizer.
45.	Crespo, Carla, et al. (författare) Ethanol production by continuous fermentation of d-(+)-cellobiose, d-(+)-xylose and sugarcane bagasse hydrolysate using the thermoanaerobe Caloramator boliviensis. 2012 Ingår i: Bioresource Technology. - : Elsevier BV. - 1873-2976 .- 0960-8524. ; 103:1, s. 186-191 Tidskriftsartikel (refereegranskat)abstract The recently isolated anaerobic bacterium Caloramator boliviensis with an optimum growth temperature of 60°C can efficiently convert hexoses and pentoses into ethanol. When fermentations of pure sugars and a pentose-rich sugarcane bagasse hydrolysate were carried out in a packed bed reactor with immobilized cells of C. boliviensis, more than 98% of substrates were converted. Ethanol yields of 0.40-0.46g/g of sugar were obtained when sugarcane bagasse hydrolysate was fermented. These features reveal interesting properties of C. boliviensis in producing ethanol from a renewable feedstock.
46.	Cucarella, Victor, et al. (författare) Effect of reactive substrates used for phosphorus removal from wastewater on the feritlity of acid soils 2008 Ingår i: Bioresource Technology. - : Elsevier BV. - 0960-8524 .- 1873-2976. ; 99:10, s. 4308-4314 Tidskriftsartikel (refereegranskat)abstract Reactive substrates used in filter systems can reduce phosphorus (P) pollution and, once saturated with P, may be recycled in agriculture. These substrates are usually calcium carbonate derivates with high pH values, which may be particularly beneficial for acid soils. Three reactive substrates (Filtra P, Polonite and wollastonite) saturated with P were used as amendments to an acid soil in a pot experiment. Substrate amendments tended to improve ryegrass yield and P uptake compared with control and potassium phosphate treatments. Polonite produced the highest yield/amendment ratio, while Polonite and Filtra P significantly increased the concentrations of P and Ca in the ryegrass. Addition of all three substrates increased the pH, AL-extractable P and cation exchange capacity of soils during the experiment. These substrates can therefore be applied to acid soils in order to recycle P and improve soil properties.
47.	Dahalan, Farrah Aini, et al. (författare) A proposed aerobic granules size development scheme for aerobic granulation process. 2015 Ingår i: Bioresource Technology. - : Elsevier BV. - 1873-2976 .- 0960-8524. ; 181, s. 291-296 Tidskriftsartikel (refereegranskat)abstract Aerobic granulation is increasingly used in wastewater treatment due to its unique physical properties and microbial functionalities. Granule size defines the physical properties of granules based on biomass accumulation. This study aims to determine the profile of size development under two physicochemical conditions. Two identical bioreactors namely Rnp and Rp were operated under non-phototrophic and phototrophic conditions, respectively. An illustrative scheme was developed to comprehend the mechanism of size development that delineates the granular size throughout the granulation. Observations on granules' size variation have shown that activated sludge revolutionised into the form of aerobic granules through the increase of biomass concentration in bioreactors which also determined the changes of granule size. Both reactors demonstrated that size transformed in a similar trend when tested with and without illumination. Thus, different types of aerobic granules may increase in size in the same way as recommended in the aerobic granule size development scheme.
48.	Damayanti, A., et al. (författare) Respirometric analysis of activated sludge models from palm oil mill effluent 2010 Ingår i: Bioresource Technology. - : Elsevier BV. - 1873-2976 .- 0960-8524. ; 101:1, s. 144-149 Tidskriftsartikel (refereegranskat)abstract Activated sludge models (ASMs) have been widely used as a basis for further model development in wastewater treatment processes. Values for parameters to be used are vital for the accuracy of the modeling approach. A continuous stirred tank reactor (CSTR), as open respirometer with continuous flow for 20 h is used in ASMs. The dissolved oxygen (DO) profile for 11 days was monitored. It was found the mass transfer coefficient K-La is 0.3 h(-1) during lag and start feed phase and 0.01 h(-1) during stop feed phase, while the heterotrophic yield coefficient Y-H is 0.44. Some of the chemical oxygen demand (COD) fractionations of palm oil mill effluent (POME) using respirometric test in ASM models are S-s 50 mg/L, S-1 16,600 mg/L, X-S 25,550 mg/L, and X-1 2,800 mg/L The comparison of experimental and ASM I from OUR concentration is found to fit well. (C) 2009 Elsevier Ltd. All rights reserved.
49.	de Albuquerque Wanderley, Maria Carolina, et al. (författare) Increase in ethanol production from sugarcane bagasse based on combined pretreatments and fed-batch enzymatic hydrolysis 2013 Ingår i: Bioresource Technology. - : Elsevier. - 0960-8524 .- 1873-2976. ; 128, s. 448-453 Tidskriftsartikel (refereegranskat)abstract Enzymatic hydrolysis of pretreated sugarcane bagasse was performed to investigate the production of ethanol. The sugarcane bagasse was pretreated in a process combining steam explosion and alkaline delignification. The lignin content decreased to 83%. Fed-batch enzymatic hydrolyses was initiated with 8% (w/v) solids loading, and 10 FPU/g cellulose. Then, 1% solids were fed at 12, 24 or 48 h intervals. After 120 h, the hydrolysates were fermented with Saccharomyces cerevisiae UFPEDA 1238, and a fourfold increase in ethanol production was reached when fed-batch hydrolysis with a 12-h addition period was used for the steam pretreated and delignified bagasse.
50.	Deng, Y., et al. (författare) Carbon availability shifts the nitrogen removal pathway and microbial community in biofilm airlift reactor 2021 Ingår i: Bioresource Technology. - : Elsevier. - 0960-8524 .- 1873-2976. ; 323 Tidskriftsartikel (refereegranskat)abstract This study investigated the response of nitrogen removal performance and microbial community to different carbon composites in biofilm airlift reactors for wastewater treatment. Three reactors were filled with poly (butylene succinate) and bamboo powder composite at the blending ratio of 9:1, 1:1 and 1:9. Increasing the component of bamboo powder in the carrier reduced the carbon availability and had an adverse effect on nitrate removal efficiency. However, bamboo powder improved the ammonia removal rate which mainly through autotrophic nitrification. Three reactors exhibited distinct microbial compositions in both bacterial and fungal diversity. High inclusion of bamboo power decreased the relative abundance of denitrifiers Denitromonas and increased the relative abundance of nitrifiers, including Nitromonas, Nitrospina and Nitrospira. Moreover, correlation network revealed a competitive interaction between the taxa responsible for ammonia removal and nitrate removal processes. Those results indicated the feasibility of steering nitrogen removal pathway through carrier formulation in wastewater treatment.

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