| 1. |
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
- Nowroozalizadeh, Salma, et al.
(författare)
-
Suppression of HIV replication in vitro by CpG and CpG conjugated to the non toxic B subunit of cholera toxin.
- 2008
-
Ingår i: Current HIV research. - : Bentham Science Publishers Ltd.. - 1873-4251 .- 1570-162X. ; 6:3, s. 230-8
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Administration of oligodeoxynucleotides (ODNs) containing CpG motifs generates a rapid and potent response of CC-chemokines, known as ligands of the HIV-1 co-receptor CCR5, in the murine female genital tract. The present study explored the potential HIV inhibitory activities of different human CpG prototypes either alone or conjugated to the non-toxic subunit of cholera toxin (CTB). Results showed that in vitro replication of both HIV-1 and HIV-2 can be suppressed by different human CpG prototypes. Importantly, the conjugation of CpG ODN to CTB (CTB-CpG) enhanced the antiviral activity of CpG against primary HIV-1 isolates of both R5 and X4 phenotypes in peripheral blood mononuclear cells (PBMC) as well as U87.CD4 co-receptor indicator cells. CTB-CpGs triggered higher amounts of MIP-1alpha, and MIP-1beta in PBMC than the corresponding CpG ODNs, which may explain the superior antiviral effect of CTB-CpG against R5 virus in PBMC. Incubation of PBMC with CpG ODN and CTB-CpG did not alter surface expression of HIV-1 receptors indicating that the observed anti-HIV-1 effect is not mediated through down regulation of HIV-1 receptors on target cells. Further, the enhanced antiviral effect of CTB-CpG was dependent on the presence of phosphorothioate backbone in the ODN, whereas the presence of CpG motif in ODNs was dispensable. These results have implications for the development of novel intervention strategies to prevent HIV infection.
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
|
|
| 8. |
|
|
| 9. |
- Harandi, Ali M, 1968, et al.
(författare)
-
Mucosal adjuvants.
- 2010
-
Ingår i: Current HIV research. - 1873-4251. ; 8:4, s. 330-5
-
Forskningsöversikt (refereegranskat)abstract
- The vast majority of pathogens invade the body through or establish infections in the mucosal tissues. Development of vaccines to combat mucosal infections represents a top priority. Mucosal immunization has recently attracted much interest as a means of generating protective immunity against mucosal pathogens. Conversely, only very few mucosal vaccines are presently approved for human use. The development of a broad range of mucosal vaccines will necessitate the development of safe and effective mucosal adjuvants and delivery systems. Over the past decade, a number of immunomodulatory agents, including toxin based adjuvants, Toll like receptor (TLR) mimetics and non TLR-targeting immunostimulators as well as delivery systems have shown promise for mucosal administration in experimental animals. However, their possible use in humans remains to be established. This paper attempts to provide a brief overview of the mucosal immunization and adjuvants with an emphasis on mucosal adjuvants in or close to clinic.
|
|
| 10. |
|
|
| 11. |
|
|
| 12. |
|
|
| 13. |
|
|
| 14. |
|
|
| 15. |
|
|
| 16. |
|
|
| 17. |
- Falk, Yana Znamenskaya, et al.
(författare)
-
Langmuir - Blodgett monolayers of SBA-15 particles with different morphologies
- 2018
-
Ingår i: Microporous and Mesoporous Materials. - : Elsevier. - 1387-1811 .- 1873-3093. ; 256, s. 32-38
-
Tidskriftsartikel (refereegranskat)abstract
- This study presents an optimized protocol for the deposition of mesoporous SBA-15 particles with platelet-like and rod-like morphologies on different types of supporting substrates using LangmuirBlodgett methodology, resulting in the formation of monolayers with porosity orientation controlled by the type, i. e. the morphology, of particles used over large areas. The morphology of the SBA-15 particles and specifically their aspect ratio are essential for the orientation of the particles and, hence, the orientation of the intrinsic porosity. Deposition on a surface, establishes a layer of SBA-15 platelets with pores oriented perpendicular to the substrate, or a layer of SBA-15 rods with pores oriented parallel to the substrate. In both cases, the oriented SBA-15 particles can be deposited onto areas that are larger than square centimeter using Langmuir-Blodgett technique. SEM characterization demonstrates formation of uniform close-packed layer of oriented mesoporous SBA-15 silica particles. Additionally, the particles coverage of the surface is independent and unaffected by the type of supporting substrate, which allows convenient experimental performance without requiring surface or particle modifications. In conclusion, Langmuir Blodgett is a convenient technique for deposition of mesoporous SBA-15 particles with different morphology types in order to obtain a close-packed layer. The methodology is suitable to create large area sensors, used in; for instance, bio-sensing applications. (C) 2017 Elsevier Inc. All rights reserved.
|
|
