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| 2. |
- Beech, Jason P., et al.
(författare)
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The separation and identification of parasite eggs from horse feces
- 2019
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Ingår i: 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. - 9781733419000 ; , s. 602-603
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Konferensbidrag (refereegranskat)abstract
- Freely grazing horses are at risk of infection by parasites such as Parascaris equorum (roundworm), Strongylus spp. (large bloodworms), Cyathostomes (small bloodworms), and Anoplochephala perfoliata (tapeworms). Mixed infections are common and diagnosis is based on demonstrations of eggs in feces followed by identification of larvae after fecal culture. Drug resistance is a growing problem, not least because treatments tend to be cheaper than diagnosis and “just in case” treatments common. There is a need for improved methods that are easy to use, rapid and cheap. Furthermore, a successful approach may find use with other livestock such as ruminants and pigs.
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| 3. |
- Beech, Jason P., et al.
(författare)
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The separation of nano-sized particles in micro-scaled post arrays
- 2019
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Ingår i: 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. - 9781733419000 ; , s. 10-11
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Konferensbidrag (refereegranskat)abstract
- The precise separation of nanoscale particles has proven challenging due to diffusion and the need to use nanoscale devices. We show the separation of particles in the 100 nm size range in Deterministic Lateral Displacement (DLD) devices with feature sizes in the 10 µm size range. We achieve this using Dielectrophoretic (DEP) forces, generated between the metal coated posts that act as active electrodes. This opens up for the separation of submicron particles based not only on size but also on electric and dielectric properties.
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| 4. |
- Broman, Axel, et al.
(författare)
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Multinodal high throughput acoustic trapping of exosomes from urine samples
- 2019
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Ingår i: 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. - 9781733419000 ; , s. 2-3
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Konferensbidrag (refereegranskat)abstract
- We report a new design of an acoustophoretic trapping device with greatly increased capacity and throughput, compared to current commercial systems. Acoustic trapping enables nanoparticle and exosome enrichment without the need for ultracentrifugation. The commercial acoustic trapping technology, AcouTrap (AcouSort AB), typically operates at flow rates < 50 μl/min which limits processing of larger samples. Using a larger capillary that supports an acoustic multi-node resonance, capacity and throughput was increased a factor 40 compared to AcouTrap. It was possible to capture and enrich exosomes from urine samples at 30 times higher flow rate than previously reported.
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| 5. |
- Guo, Weijin, et al.
(författare)
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Synthetic microfluidic paper with superior fluorescent signal readout
- 2019
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Ingår i: Proceedings of The 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences. - 9781733419000 ; , s. 1056-1057
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Konferensbidrag (refereegranskat)abstract
- This work is the first report on the use of synthetic microfluidic paper for lateral flow immunoassays. We grafted test lines of biotin on the synthetic paper using the thiol-yne “click” reaction. We captured fluorescently labeled streptavidin in a lateral flow fashion. Our two main findings are that, compared to other polymer lateral flow substrates with similar surface area, the synthetic microfluidic paper geometry results in 1) a stronger and more stable fluorescent signal per capture area, and 2) a sensitivity ~7 times higher.
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| 6. |
- Ho, Bao D., et al.
(författare)
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Viable/non-viable cell assay using electrokinetic deterministic lateral displacement
- 2019
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Ingår i: 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. - 9781733419000 ; , s. 596-597
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Konferensbidrag (refereegranskat)abstract
- To evaluate the effect of antibiotic drugs, it is important that the cells that are killed can be sorted from the cells that are resistant to the antibiotics. Here we report a simple label-free method for the continuous separation of viable/non-viable cells using Electrokinetic Deterministic Lateral Displacement. In general, this opens up for continuous sorting of a population of cells that is homogeneous in size but heterogeneous in surface charge or dielectric properties, properties that change as a function of viability.
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| 7. |
- Nyblom, My, 1995, et al.
(författare)
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Bacterial identification by optical mapping of genomic DNA in nanofluidic channels
- 2019
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Ingår i: 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. - 9781733419000 ; , s. 821-822
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Konferensbidrag (refereegranskat)abstract
- A variety of pathogenic bacteria can infect humans and the increase in bacteria resistant to common antibiotics is a large threat to human health worldwide. This work presents a method, based on optical DNA mapping (ODM) in nanofluidic channels, that can detect the type of bacterial present in a sample by matching the obtained maps of large DNA molecules to a database of fully assembled bacterial genomes. The extraction and labelling protocol has been designed to work for both Gram-positive and Gram-negative bacteria, not requiring any prior knowledge about the sample content.
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| 8. |
- Persson, Henrik, et al.
(författare)
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Rapid fabrication of PMMA/PET-E/PMMA for thermoplastic microfluidic membrane devices
- 2019
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Ingår i: 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. - 9781733419000 ; , s. 1666-1667
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Konferensbidrag (refereegranskat)abstract
- We present a method to rapidly fabricate a large number of thermoplastic microfluidic systems where horizontal microchannels are separated by a porous membrane for modelling biological barriers. We optimize and combine solvent bonding, milling and laser cutting to fabricate in excess of 100 devices in two days. The optimized protocol relies on a commercial solvent blend, retention grooves for solvent distribution, and force application to create strong, leak-free bonds. The devices are evaluated for suitability for modelling biological barriers by culturing endothelial cells and measuring permeability using a plate reader.
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| 9. |
- Ström, Oskar E., et al.
(författare)
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Long DNA isolation and imaging using lateral displacement arrays integrated with DNA combing
- 2019
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Ingår i: 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. - 9781733419000 ; , s. 771-772
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Konferensbidrag (refereegranskat)abstract
- We demonstrate proof of principle of an efficient and simple method combining sample preparation and stretching of DNA in a single device that can be used as basis for an integrated portable system for optical DNA mapping. An important bottleneck for DNA mapping, as well as for other methods, is the isolation of long DNA molecules from a background of contaminants such as shorter DNA fragments, dyes, and lysate. We demonstrate the isolation of longer (48.5 kbp or 166 kbp) from shorter (<10 kbp) DNA strands using Deterministic Lateral Displacement (DLD), followed by surface-stretching of the sorted DNA for mapping.
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