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- Erlingsdottir, Gudbjörg, et al.
(author)
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eHealth services, patient empowerment and professional accountability - An empirical study on the changing patient-doctor relationship in the digital world
- 2014
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In: ; , s. 1-21
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Conference paper (peer-reviewed)abstract
- In Sweden, as in many other European countries, government and public agencies have promoted the expansion of eHealth over the past years, arguing that this development enhances patient participation, empowerment and cost efficiency. The development is part of a more comprehensive movement, emphasizing patient’s rights including options to make far reaching decisions concerning the patient’s own care and treatment. This study focuses on the deployment of one such eHealth service – the patients’ digital access to their own medical records over the Internet. The use of electronic patient records (EPRs) is reported as being one of the most important services within the development of eHealth services and constitute a prominent part of the movement towards computerization in healthcare overall. In November 2012, the eHealth service “My medical record on the internet” was introduced in Uppsala County Council and the same civic service was introduced in another Swedish county council, Region Skåne, in March 2014. Both launches have caused resistance and, in some cases, strong negative reactions among many of the medical professionals. Simultaneously, the actions of the medical professionals have been discussed as well as criticized by other actors participating in the development process.
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| 2. |
- Kadekar, Sandeep, et al.
(author)
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Synthetic design of asymmetric miRNA with engineered 3′-overhang to improve strand selection
- 2019
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In: Molecular Therapy Nucleic Acids. - : Elsevier. - 2162-2531. ; 16, s. 597-604
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Journal article (peer-reviewed)abstract
- We have developed a novel miRNA design that significantly improves strand selection within the RISC complex by engineering the 3′-end by adding extra nucleotides. Addition of seven nucleotides at the 3′-ends of the miR or miR* strand resulted in a thermodynamic asymmetry at either of the two-ends, which resulted in selective RISC recruitment as demonstrated by the stem-loop quantitative PCR experiment. Such selective recruitment was also corroborated at the protein level by Western blot analysis. In order to investigate the functional effect due to selective recruitment, we performed apoptosis and metastasis studies using human colon carcinoma cells (HCT116) and human osteosarcoma cells (MG63). These experiments indicated that the recruitment of miR strand is responsible for inducing apoptosis as well as to inhibit invasiveness of cancer cells. Recruitment of miR* strand, on the other hand, showed opposite effect. To the best of our knowledge, our strand engineering strategy is the first report of improved strand selection of desired miRNA strand by RISC without using any chemical modifications or mismatches. We believe such structural modifications of miR34a could mitigate some of the off-target effects of miRNA therapy and would also allow a better understanding of sequence-specific gene regulation. Such a design could also be adapted to other miRNA to enhance their therapeutic potential.
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