1. |
- Molin, Magnus, et al.
(författare)
-
Two Novel Adenovirus Vector Systems Permitting Regulated Protein Expression in Gene Transfer Experiments
- 1998
-
Ingår i: Journal of Virology. - 0022-538X .- 1098-5514. ; 72:10, s. 8358-8861
-
Tidskriftsartikel (refereegranskat)abstract
- Two new adenovirus vector systems based on the tetracycline-regulated Tet-ON- (Gossen, M., et al., Science 268:1766-1769, 1995) and the RU 486-regulated progesterone antagonist (Wang, Y., et al., Proc. Natl. Acad. Sci. USA 91:8180-8184, 1994)-induced gene expression systems are described. We show that both systems permit a tight control of chloramphenicol acetyltransferase reporter gene expression in a variety of cell types, with induction levels of approximately 1,800-fold (Tet-ON system) and 600-fold (RU 486-regulated system), respectively. A significant advantage of our vector systems is that reporter protein expression can be adjusted over a wide range by varying the amount of inducer. The Tet-ON system is also shown to permit an efficient control of reporter gene expression in mice.
|
|