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- Hüttner, Silvia, 1984, et al.
(författare)
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Recent advances in the intellectual property landscape of filamentous fungi
- 2020
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Ingår i: Fungal Biology and Biotechnology. - : Springer Science and Business Media LLC. - 2054-3085. ; 7:1
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Forskningsöversikt (refereegranskat)abstract
- For centuries, filamentous fungi have been used in the making of food and beverages, and for decades for the production of enzymes and pharmaceuticals. In the last decades, the intellectual property (IP) landscape for fungal technology has seen an ever increasing upward trend, introducing new and promising applications utilising fungi. In this review, we highlight fungi-related patent applications published during the last 5 years (2015–2020), identify the key players in each field, and analyse future trends. New developments in the field of fungal technology include the increased use of filamentous fungi as a food source (mycoprotein), using fungi as biodegradable materials, in wastewater treatment, in integrated biorefineries and as biological pest agents. Biotechnology companies in Europe and the US are currently leading when it comes to the number of patents in these areas, but Asian companies and research institutes, in particular in China, are becoming increasingly important players, for example in pesticide formulation and agricultural practices.
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| 2. |
- Shin, Jae Ho, 1987, et al.
(författare)
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Exploring functionality of the reverse β-oxidation pathway in Corynebacterium glutamicum for production of adipic acid
- 2021
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Ingår i: Microbial Cell Factories. - : Springer Science and Business Media LLC. - 1475-2859. ; 20:1
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Tidskriftsartikel (refereegranskat)abstract
- Background: Adipic acid, a six-carbon platform chemical mainly used in nylon production, can be produced via reverse β-oxidation in microbial systems. The advantages posed by Corynebacterium glutamicum as a model cell factory for implementing the pathway include: (1) availability of genetic tools, (2) excretion of succinate and acetate when the TCA cycle becomes overflown, (3) initiation of biosynthesis with succinyl-CoA and acetyl-CoA, and (4) established succinic acid production. Here, we implemented the reverse β-oxidation pathway in C. glutamicum and assessed its functionality for adipic acid biosynthesis. Results: To obtain a non-decarboxylative condensation product of acetyl-CoA and succinyl-CoA, and to subsequently remove CoA from the condensation product, we introduced heterologous 3-oxoadipyl-CoA thiolase and acyl-CoA thioesterase into C. glutamicum. No 3-oxoadipic acid could be detected in the cultivation broth, possibly due to its endogenous catabolism. To successfully biosynthesize and secrete 3-hydroxyadipic acid, 3-hydroxyadipyl-CoA dehydrogenase was introduced. Addition of 2,3-dehydroadipyl-CoA hydratase led to biosynthesis and excretion of trans-2-hexenedioic acid. Finally, trans-2-enoyl-CoA reductase was inserted to yield 37 µg/L of adipic acid. Conclusions: In the present study, we engineered the reverse β-oxidation pathway in C. glutamicum and assessed its potential for producing adipic acid from glucose as starting material. The presence of adipic acid, albeit small amount, in the cultivation broth indicated that the synthetic genes were expressed and functional. Moreover, 2,3-dehydroadipyl-CoA hydratase and β-ketoadipyl-CoA thiolase were determined as potential target for further improvement of the pathway.
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| 3. |
- Shin, Jae Ho, 1987, et al.
(författare)
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Synthetic pathway engineering in Corynebacterium glutamicum for production of adipic acid
- 2020
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- We are interested in production of adipic acid from renewable sources using a gram-positive bacterium, Corynebacterium glutamicum ATCC 13032, as a target for metabolic engineering. We aim to construct a synthetic pathway that would allow biosynthesis of adipic acid and to implement it into the host chassis for utilizing simple sugar, glucose, as the starting raw material. The chosen pathway stems off from intermediates from the central metabolism and requires 5 synthetic biochemical steps before reaching adipic acid. The pathway is equipped with a promiscuous enzyme to allow leakage at each metabolic step and byproduct formation in order to monitor the efficiency of each step of the pathway. In order to ensure translation, each gene introduced was codon-optimized to C. glutamicum. Introducing the synthetic genes one by one into C. glutamicum as well as flask-level cultivation of engineered strains in a semi-defined medium allowed detection of each byproduct of the pathway leading up to adipic acid by GC/MS based methods. Further, the codon-optimized version of the 5 synthetic genes were constructed in 2 separate operons each with an inducible promoter. Finally, we analyzed the translational efficiency of the genes and modified the constructs for a better operon expression. We implement additional sample preparation methods for isolating and concentrating adipic acid content for better analysis. The results shown here will be used to further develop and complete biosynthesis of adipic acid from a C. glutamicum chassis.
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