| 1. |
- Crafoord, Sven, 1950-, et al.
(författare)
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Photoreceptor survival in transplantation of autologous iris pigment epithelial cells to the subretinal space
- 2002
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Ingår i: Acta Ophthalmologica Scandinavica. - : Wiley. - 1395-3907 .- 1600-0420. ; 80:4, s. 387-394
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: To investigate photoreceptor survival in transplantation of non-cultured iris pigment epithelial (IPE) cells to the subretinal space in a prospective experimental study. Methods: Upper iridectomies were carried out in the right eyes of 37 pigmented rabbits. Suspensions of freshly harvested autologous IPE cells (without culturing) were prepared and injected into the subretinal space of the same eye. Follow-up examinations were carried out using ophthalmoscopy and colour fundus photography. The rabbits were killed at 1, 2, 3 and 6 months, respectively, and the eyes examined with light and electron microscopy. Results: On histological examination, the photoreceptor cells were found to be well-preserved in grafted areas at 1-3 months. At 6 months, the photoreceptors generally disclosed a normal nuclear layer and long outer segments when overlying areas with single cells or clusters of transplanted IPE cells. Multilayers of cells in abundance, including native RPE cells and macrophages (stained with RAM 11), particularly under microfolds of the neural retina, were occasionally associated with photoreceptor damage and nuclear drop out from the outer retinal layer. There was no inflammatory response in the choroid and the choriocapillaris remained patent. Conclusion: The experiments show that grafting freshly harvested autologous IPE cells to the subretinal space is feasible and that the photoreceptors generally survive for at least 6 months when overlying the transplanted areas. Multi-layers of abundant cells in the subretinal space may induce adverse focal effects on adjacent photoreceptors.
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| 2. |
- Crafoord, Sven, et al.
(författare)
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Transplantation of Autologous Iris Pigment Epithelial Cells To the Subretinal Space : II. Photoreceptor Survival and Consequences of Cellular Multilayers
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Purpose: To study the effects of transplanted iris pigment epithelial (IPE) cells in the subretinal space on the survival of adjacent photoreceptors.Methods: An upper iridectomy was made on the right eye of 37 pigmented rabbits. Suspensions of autologous IPE cells were prepared and injected into the subretinal space of the same eye. Follow- up examinations was performed using ophthalmoscopy and calor fundus photography. At 1, 2, 3 and 6 months, respectively, the rabbits were sacrificed and the eyes examined with light and electron microscopy.Results: On histological examination, the photoreceptor cells were well preserved in grafted areas at 1-3 months. At 6 months, also, the photoreceptors generally disclosed a normal nuclear layer and long outer segments when overlying areas with single cells or clusters of IPE. However, multilayers of transplanted I PE, native RPE cells and macrophages in the subretinal space were frequently associated with photoreceptor damage and nuclear drop out from the outer retinal layer.Conclusion: In this experimental model, the photoreceptors generally survive adjacent to grafted IPE cells for 6 months. Multilayers of subretinal cells are likely to induce adverse effects on adjacent photoreceptors which is a new finding that requires further investigation.
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| 3. |
- Crafoord, Sven, et al.
(författare)
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Transplantation of Autologous Iris Pigment Epithelial Cells to the Subretinal Space : I. Morphological Features
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Purpose: To investigate the cellular morphology in the subretinal space following transplantation of iris pigment epithelial (IPE) cells from the same eye.Methods: Following an iridectomy, fresh IPE cells were prepared. After pars plana vitrectomy, a suspension of autologous IPE cells was injected into the subretinal space in 37 rabbits. The grafts were monitored by ophthalmoscopy and calor fundus photography. Rabbits were sacrificed at 1, 2, 3 and 6 months, respectively, and the eyes examined with light and electron microscopy.Results: The grafted area retained the same configuration over 6 months but then appeared less pigmented. At 1-3 months, the IPE formed one or more contiguous layers on top of native RPE. At 6 months, cells compatible with grafted IPE were present in the subretinal space forming mono-layer like chains integrating with the native RPE. Depigmented cells of presumed IPE origin were seen. lt was commonly observed that the apical portion of RPE cells disclosed abundant melanin granules. With time the grafted cells appeared to decrease in number but focal clusters of IPE cells and large macrophages were present.Conclusion: Transplanted IPE cells survived for up to 6 months in the subretinal space. Our observations suggest a scenario of remodeling of the cellular layers in the subretinal space over time where grafted IPE cells formed a compound layer with the native RPE. Transplantation of autologous IPE cells may have a potential as a treatment modality in selected cases of age-related macular degeneration with impending degeneration of RPE and choriocapillaris.
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| 4. |
- Heijl, Anders, et al.
(författare)
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Nordic research in ophthalmology.
- 2005
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Ingår i: Acta ophthalmologica Scandinavica. - : Wiley. - 1395-3907 .- 1600-0420. ; 83:3, s. 278-88
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Nordic ophthalmologists and vision scientists are active in many fields of eye research. This is most evident at the biannual Nordic Congress of Ophthalmology, most recently held in Malmö in June 2004. The authors here review some of the research in vision and ophthalmology presented at this meeting or published recently by Nordic scientists. This paper does not represent a comprehensive review of all Nordic research in the field, but attempts to give an overview of some of the activities underway in eye research in this part of the world.
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| 5. |
- Söderberg, Anne-Catherine, et al.
(författare)
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Combination therapy with low-dose transpupillary thermotherapy and intravitreal ranibizumab for neovascular age-related macular degeneration : a 24-month prospective randomised clinical study
- 2012
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Ingår i: British Journal of Ophthalmology. - : BMJ. - 0007-1161 .- 1468-2079. ; 96:5, s. 714-718
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Tidskriftsartikel (refereegranskat)abstract
- AIM: To compare the effect of combined low-dose transpupillary thermotherapy (TTT) and intravitreal ranibizumab with sham TTT and intravitreal ranibizumab in patients with neovascular age-related macular degeneration (AMD). METHODS: A 24-month, double-masked, randomised, active-controlled clinical trial. 100 patients with primary neovascular AMD were randomly assigned (1:1) to receive intravitreal ranibizumab and sham TTT or intravitreal ranibizumab and low-dose TTT. After an initial loading phase of ranibizumab patients were assigned to receive quarterly low-dose TTT (136 mW/mm) or sham TTT for 24 months. Retreatment with ranibizumab was allowed in both treatment groups using a variable dosing regimen. The primary endpoint was the number of intravitreal injections with ranibizumab. Secondary endpoints included change in best corrected visual acuity (BCVA), central retinal thickness (CRT) and lesion area. RESULTS: In the per protocol (PP) population (78 patients) the mean number of ranibizumab injections was 8.0 in the sham TTT group versus 6.3 in the TTT group (p<0.05). The mean number of injections between 0-12 months and 13-24 months was 4.8 versus 4.6 (p>0.05) and 3.2 versus 1.7 (p<0.01) in the sham TTT and TTT groups, respectively. There was no statistically significant difference in BCVA (+4.0 vs +0.9 ETDRS letters), CRT (-49.9% vs -36.4%) or lesion area (-0.3% vs -10.6%) between the treatment groups at the final examination. The results of the intent-to-treat population (92 patients) were similar to the PP population. CONCLUSIONS: Treatment with low-dose TTT significantly reduced the number or intravitreal injections of ranibizumab over 24 months. The results suggest that low-dose TTT can serve as an adjuvant in combination with intravitreal ranibizumab for neovascular AMD.
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