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| 2. |
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| 3. |
- Andersson, Claes-Henrik, 1980-
(author)
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Chemistry of Carbon Nanostructures : Functionalization of Carbon Nanotubes and Synthesis of Organometallic Fullerene Derivatives
- 2011
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Doctoral thesis (other academic/artistic)abstract
- This thesis is based on two main parts. The first part concerns purification and functionalization of carbon nanotubes (papers I-III), and the second part is related to the synthesis of organometallic fullerene derivatives (papers IV-VII): Two oxidative methods involving aqueous nitric acid were compared with respect to their capability to introduce carboxylic groups into single walled carbon nanotubes, and several literature methods for esterification and amidation of these groups have been evaluated with focus on efficiency and reproducibility in forming covalently functionalized products soluble in organic media. Amidation proceeding via a SWNT-(COCl)n intermediate yielded the expected covalent product, whereas carboxylate salt formation dominated with other attempted methods. Esterification was achieved via the acyl chloride method and via alkylation of SWNT-(COO–)n, the latter being the more efficient method. A new, reagent-free method for purification of single- and multi walled carbon nanotubes has been developed. Microwave treatment dissociates non-nanotube carbon and disperses it into an organic solvent, resulting in very pure carbon nanotubes within a few minutes of heating, without the involvement of acidic/oxidative reagents. According to thermogravimetric analysis, Raman and IR spectroscopy, as well as SEM, the process yields nanotubes with a low degree of defects. A non-covalent approach has been employed to prepare nanotubes functionalized with glycosides. Derivatives of galactose and lactose were covalently linked to a pyrene moiety and the thus formed pyrene-glycosides were non-covalently attached to single- and multi walled carbon nanotubes by π-π interactions. Fluorescence titrations have been used to quantify the formed supramolecular assemblies, which for SWNTs exhibits increased water solubility. A fulleropyrrolidine-(tricarbonyl)chromium complex was synthesized and fully characterized. IR spectroelectrochemistry was used to probe the redox state of the fullerene and provided evidence for electronic communication between the two electroacive moieties. A C60-ferrocene-C60 triad system was synthesized and characterized. Cyclic voltammetry and fluorescence studies suggested electronic communication between ferrocene and the two fullerenes. Finally, the synthesis and initial characterization of short fullerene-ferrocene oligomers are presented.
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| 4. |
- Andersson, Claes-Henrik, et al.
(author)
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Reproducibility and efficiency of carbon nanotube end-group generation and functionalization
- 2009
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In: European Journal of Organic Chemistry. - : Wiley. - 1434-193X .- 1099-0690. ; 26, s. 4421-4428
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Journal article (peer-reviewed)abstract
- In a systematic fashion, several methods for esterification and amidation of single-walled carbon nanotubes have been evaluated with focus on efficiency and reproducibility in forming covalently functionalized products soluble in organic media. The outcome of transformations was determined using IR, Raman and NMR spectroscopy and by thermogravimetric analysis (TGA). Amidation proceeding via a SWNT-(COCl)(n) intermediate yielded the expected covalent product, whereas carboxylate salt formation dominated with other attempted methods. Esterification was achieved via the acyl chloride method and via alkylation of SWNT-(COO-)(n), the latter being the more efficient method. A non-covalent solubilizing interaction was obtained for RNH2 but not for ROH (R = octadecyl), proving that the most important non-covalent interaction between oxidatively cleaned SWNTs and octadecylamine is a salt formation. The outcome of the secondary functionalization of carboxyl units is highly reproducible for experiments carried out on the same batch of SWNT-(COOH)(n). Normalization of the outcome of the secondary functionalization to the composition of the different batches of starting materials reveals an overall high reproducibility of the secondary function alizations. The differences in outcome related to different commercial SWNT batches from the same synthetic procedure is negligible compared to that resulting from differences in overall carboxyl content after the primary HNO3 oxidative cleaning step. Hence, the composition of purified SWNT starting materials always needs to be assessed, in particular before drawing any conclusions concerning differences in outcome from reaction systems involving different sources of SWNT material.
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| 5. |
- Andersson, Claes-Henrik, et al.
(author)
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Reversible Non-Covalent Derivatisation of Carbon Nanotubes with Glycosides
- 2009
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In: Soft Matter. - 1744-683X. ; 5:14, s. 2713-2716
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Journal article (peer-reviewed)abstract
- SWNTs and MWNTs have been non-covalently functionalized with glycosides in a reversible manner, and fluorescence titrations have been used to quantify the formed supramolecular assemblies which for SWNTs exhibits increased water solubility.
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| 6. |
- Andersson, Claes-Henrik, et al.
(author)
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Reversible non-covalent derivatisation of carbon nanotubes with glycosides
- 2009
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In: Soft Matter. - : Royal Society of Chemistry (RSC). - 1744-683X .- 1744-6848. ; 5:14, s. 2713-2716
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Journal article (peer-reviewed)abstract
- SWNTs and MWNTs have been non-covalently functionalized with glycosides in a reversible manner, and fluorescence titrations have been used to quantify the formed supramolecular assemblies which for SWNTs exhibits increased water solubility.
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| 7. |
- Andersson, Claes-Henrik, 1980-, et al.
(author)
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Short ferrocene-[60]fulleropyrrolidine oligomers. A preliminary account on synthetic studies
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Other publication (other academic/artistic)abstract
- A synthetic strategy towards short fullerene based organometallic oligomers is reported. The synthetic approach is based on the secondary functionalization of N-unsubstituted fulleropyrrolidines with ferrocene dicarboxylic acid chloride. Preliminary characterization by mass spectrometry, UV/Vis and NMR suggest a trimer or tetramer structure.
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| 8. |
- Andersson, Claes-Henrik, 1980-, et al.
(author)
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Synthesis and characterization of a ferrocene-linked bis-fullerene[60] dumbbell
- 2012
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In: Dalton Transactions. - 1477-9226 .- 1477-9234. ; 41:8, s. 2374-2381
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Journal article (peer-reviewed)abstract
- A new [60]fullerene dumbbell consisting of two fulleropyrrolidines connected to a central ferrocene unit by amide linkages has been prepared and fully characterized by elemental analysis, 1H NMR, UV/Vis, fluorescence and mass spectrometry. The electrochemical properties as determined by cyclic voltammetry show ground state electronic communication between the ferrocene and the fullerene units. In addition, the preparaton of a ferrocene building block for an alternative linking approach is presented.
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| 9. |
- Andersson, Claes-Henrik, et al.
(author)
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Synthesis and IR Spectroelectrochemical Studies of a [60]Fulleropyrrolidine-(tricarbonyl)chromium Complex : Probing C-60 Redox States by IR Spectroscopy
- 2011
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In: European Journal of Inorganic Chemistry. - : John Wiley & Sons. - 1434-1948 .- 1099-1948 .- 1099-0682. ; :11, s. 1744-1749
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Journal article (peer-reviewed)abstract
- The synthesis of a new fulleropyrrolidine-(tricarbonyl)chromium complex: 1-methyl-2-(4-methoxyphenyl)-3,4-[60]fulleropyrrolidine-(tricarbonyl)chromium is described together with its characterization by IR, NMR and cyclic voltammetry. IR spectro-electrochemistry has been used to probe the redox level of the fullerene derivative via the relative position of the vibrational bands of the CO ligands, which are sensitive to the electronic state of the complex. Other strategies to incorporate a tricarbonylchromium moiety to fullerene C60 are also briefly discussed and evaluated.
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| 10. |
- Andersson, Henrik, et al.
(author)
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The effect of mechanical stress on lateral-effect position sensitive detector characteristics
- 2006
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In: Nuclear Instruments and Methods in Physics Research Section A. - : Elsevier BV. - 0168-9002 .- 1872-9576. ; 563:1, s. 150-154
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Journal article (peer-reviewed)abstract
- Position-sensitive detectors (PSDs) are widely used in noncontact measurement systems. In order to minimize the size of such systems, interest has increased in mounting the PSD chip directly onto printed circuit boards (PCBs). Stress may be induced in the PSD because of the large differences in thermal expansion coefficients, as well as the long-term geometrical stability of the chip packaging. Mechanical stress has previously been shown to have an effect on the performance of semiconductors. The accuracy, or linearity, of a lateral effect PSD is largely dependent on the homogeneity of the resistive layer. Variations of the resistivity over the active area of the PSD will result in an uneven distribution of photo-generated current, and hence an error in the readout position. In this work experiments were performed to investigate the influence of anisotropic mechanical stress in terms of nonlinearity. PSD chips of 60×3 mm active area were subjected, respectively, to different amounts of compressive and tensile stress to determine the influence on the linearity.
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| 11. |
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| 12. |
- Blom, Magnus, et al.
(author)
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Photomodulable bis-porphyrin molecular tweezers as dynamic host systems for diamine guests
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Other publication (other academic/artistic)abstract
- Bisporphyrin molecular tweezers with an enediyne (1) or a stiff stilbene (2) photoswitchable spacer are proposed as systems for modulation of bitopic binding to diamine guests via E/Z photoisomerization. The photoisomerization has been monitored by UV-Vis and 1H NMR spectroscopy and occurs without side reactions such as Bergman cyclization. Possible applications are rationalized in terms of competitive binding involving monoamine/diamine mixtures, and are supported by conformational analysis of the envisioned host-guest complexes. Binding dynamics for conformationally flexible guests show significantly different performance of aliphatic 1,w-diamine guests with varying N-N distance.
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| 13. |
- Blom, Magnus, 1984-, et al.
(author)
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Synthesis and Properties of Bis-Porphyrin Molecular Tweezers : Effects of Spacer Flexibility on Binding and Supramolecular Chirogenesis
- 2016
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In: Molecules. - : MDPI AG. - 1431-5157 .- 1420-3049. ; 21:1
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Journal article (peer-reviewed)abstract
- Abstract: Ditopic binding of various dinitrogen compounds to three bisporphyrin molecular tweezers with spacers of varying conformational rigidity, incorporating the planar ene-diyne (1), the helical stiff stilbene (2), or the semirigid glycoluril motif fused to the porphyrins (3) are compared. Binding constants Ka = 10^4 to 10^6 M^-1 reveal subtle differences between these tweezers, that are discussed in terms of porphyrin dislocation modes. Exciton coupled circular dichroism (ECCD) of complexes with chiral dinitrogen guests provides experimental evidence for the conformational properties of the tweezers. The results are further supported and rationalized by conformational analysis.
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| 14. |
- Bohm, Felix, et al.
(author)
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FFR-Guided Complete or Culprit-Only PCI in Patients with Myocardial Infarction
- 2024
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In: New England Journal of Medicine. - : MASSACHUSETTS MEDICAL SOC. - 0028-4793 .- 1533-4406.
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Journal article (peer-reviewed)abstract
- Background The benefit of fractional flow reserve (FFR)-guided complete revascularization in patients with ST-segment elevation myocardial infarction (STEMI) and multivessel coronary artery disease remains unclear.Methods In this multinational, registry-based, randomized trial, we assigned patients with STEMI or very-high-risk non-STEMI (NSTEMI) and multivessel disease who were undergoing primary percutaneous coronary intervention (PCI) of the culprit lesion to receive either FFR-guided complete revascularization of nonculprit lesions or no further revascularization. The primary outcome was a composite of death from any cause, myocardial infarction, or unplanned revascularization. The two key secondary outcomes were a composite of death from any cause or myocardial infarction and unplanned revascularization.Results A total of 1542 patients underwent randomization, with 764 assigned to receive FFR-guided complete revascularization and 778 assigned to receive culprit-lesion-only PCI. At a median follow-up of 4.8 years (interquartile range, 4.3 to 5.2), a primary-outcome event had occurred in 145 patients (19.0%) in the complete-revascularization group and in 159 patients (20.4%) in the culprit-lesion-only group (hazard ratio, 0.93; 95% confidence interval [CI], 0.74 to 1.17; P=0.53). With respect to the secondary outcomes, no apparent between-group differences were observed in the composite of death from any cause or myocardial infarction (hazard ratio, 1.12; 95% CI, 0.87 to 1.44) or unplanned revascularization (hazard ratio, 0.76; 95% CI, 0.56 to 1.04). There were no apparent between-group differences in safety outcomes.Conclusions Among patients with STEMI or very-high-risk NSTEMI and multivessel coronary artery disease, FFR-guided complete revascularization was not shown to result in a lower risk of a composite of death from any cause, myocardial infarction, or unplanned revascularization than culprit-lesion-only PCI at 4.8 years. (Funded by the Swedish Research Council and others; FULL REVASC ClinicalTrials.gov number, NCT02862119.) In a registry-based trial, FFR-guided PCI of nonculprit lesions did not result in a lower risk of a composite of death from any cause, myocardial infarction, or unplanned revascularization than culprit-lesion-only PCI.
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| 15. |
- Böhm, Felix, et al.
(author)
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FFR-Guided Complete or Culprit-Only PCI in Patients with Myocardial Infarction
- 2024
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In: New England Journal of Medicine. - : Massachusetts Medical Society. - 0028-4793 .- 1533-4406. ; 390:16, s. 1481-1492
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Journal article (peer-reviewed)abstract
- BACKGROUND: The benefit of fractional flow reserve (FFR)-guided complete revascularization in patients with ST-segment elevation myocardial infarction (STEMI) and multivessel coronary artery disease remains unclear. METHODS: In this multinational, registry-based, randomized trial, we assigned patients with STEMI or very-high-risk non-STEMI (NSTEMI) and multivessel disease who were undergoing primary percutaneous coronary intervention (PCI) of the culprit lesion to receive either FFR-guided complete revascularization of nonculprit lesions or no further revascularization. The primary outcome was a composite of death from any cause, myocardial infarction, or unplanned revascularization. The two key secondary outcomes were a composite of death from any cause or myocardial infarction and unplanned revascularization. RESULTS: A total of 1542 patients underwent randomization, with 764 assigned to receive FFR-guided complete revascularization and 778 assigned to receive culprit-lesion-only PCI. At a median follow-up of 4.8 years (interquartile range, 4.3 to 5.2), a primary-outcome event had occurred in 145 patients (19.0%) in the complete-revascularization group and in 159 patients (20.4%) in the culprit-lesion-only group (hazard ratio, 0.93; 95% confidence interval [CI], 0.74 to 1.17; P = 0.53). With respect to the secondary outcomes, no apparent between-group differences were observed in the composite of death from any cause or myocardial infarction (hazard ratio, 1.12; 95% CI, 0.87 to 1.44) or unplanned revascularization (hazard ratio, 0.76; 95% CI, 0.56 to 1.04). There were no apparent between-group differences in safety outcomes. CONCLUSIONS: Among patients with STEMI or very-high-risk NSTEMI and multivessel coronary artery disease, FFR-guided complete revascularization was not shown to result in a lower risk of a composite of death from any cause, myocardial infarction, or unplanned revascularization than culprit-lesion-only PCI at 4.8 years. (Funded by the Swedish Research Council and others; FULL REVASC ClinicalTrials.gov number, NCT02862119.).
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| 16. |
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| 17. |
- Chajara, Khalil, et al.
(author)
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The reagent-free, microwave-assisted purification of carbon nanotubes
- 2010
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In: New Journal of Chemistry. - : Royal Society of Chemistry (RSC). - 1144-0546 .- 1369-9261. ; 34:10, s. 2275-2280
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Journal article (peer-reviewed)abstract
- We have developed a microwave-assisted, reagent-free method for the efficient primary purification of MW and SW carbon nanotubes that is extremely fast compared to previously reported processes. The treatment dissociates and disperses non-nanotube carbon in an organic solvent to yield very pure carbon nanotubes within a few minutes of heating and a simple filtration, without the involvement of acidic/oxidative reagents. According to thermogravimetric analysis, Raman and IR spectroscopy, as well as scanning and transmission electron microscopy, the process yields pure nanotubes with a low degree of defects.
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| 18. |
- Enroth, Stefan, et al.
(author)
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Nucleosome regulatory dynamics in response to TGF beta
- 2014
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In: Nucleic Acids Research. - : Oxford University Press (OUP). - 0305-1048 .- 1362-4962. ; 42:11, s. 6921-6934
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Journal article (peer-reviewed)abstract
- Nucleosomes play important roles in a cell beyond their basal functionality in chromatin compaction. Their placement affects all steps in transcriptional regulation, from transcription factor (TF) binding to messenger ribonucleic acid (mRNA) synthesis. Careful profiling of their locations and dynamics in response to stimuli is important to further our understanding of transcriptional regulation by the state of chromatin. We measured nucleosome occupancy in human hepatic cells before and after treatment with transforming growth factor beta 1 (TGFβ1), using massively parallel sequencing. With a newly developed method, SuMMIt, for precise positioning of nucleosomes we inferred dynamics of the nucleosomal landscape. Distinct nucleosome positioning has previously been described at transcription start site and flanking TF binding sites. We found that the average pattern is present at very few sites and, in case of TF binding, the double peak surrounding the sites is just an artifact of averaging over many loci. We systematically searched for depleted nucleosomes in stimulated cells compared to unstimulated cells and identified 24 318 loci. Depending on genomic annotation, 44-78% of them were over-represented in binding motifs for TFs. Changes in binding affinity were verified for HNF4α by qPCR. Strikingly many of these loci were associated with expression changes, as measured by RNA sequencing.
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| 19. |
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| 20. |
- Gezelius, Henrik, PhD, 1977-, et al.
(author)
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Comparison of high-throughput single-cell RNA-seq methods for ex vivo drug screening
- 2024
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In: NAR Genomics and Bioinformatics. - : Oxford University Press. - 2631-9268. ; 6:1
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Journal article (peer-reviewed)abstract
- Functional precision medicine (FPM) aims to optimize patient-specific drug selection based on the unique characteristics of their cancer cells. Recent advancements in high throughput ex vivo drug profiling have accelerated interest in FPM. Here, we present a proof-of-concept study for an integrated experimental system that incorporates ex vivo treatment response with a single-cell gene expression output enabling barcoding of several drug conditions in one single-cell sequencing experiment. We demonstrate this through a proof-of-concept investigation focusing on the glucocorticoid-resistant acute lymphoblastic leukemia (ALL) E/R+ Reh cell line. Three different single-cell transcriptome sequencing (scRNA-seq) approaches were evaluated, each exhibiting high cell recovery and accurate tagging of distinct drug conditions. Notably, our comprehensive analysis revealed variations in library complexity, sensitivity (gene detection), and differential gene expression detection across the methods. Despite these differences, we identified a substantial transcriptional response to fludarabine, a highly relevant drug for treating high-risk ALL, which was consistently recapitulated by all three methods. These findings highlight the potential of our integrated approach for studying drug responses at the single-cell level and emphasize the importance of method selection in scRNA-seq studies. Finally, our data encompassing 27 327 cells are freely available to extend to future scRNA-seq methodological comparisons.
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| 21. |
- Håkansson, Claes, et al.
(author)
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Inter-modality assessment of medial temporal lobe atrophy in a non-demented population: application of a visual rating scale template across radiologists with varying clinical experience
- 2022
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In: European Radiology. - : Springer Science and Business Media LLC. - 0938-7994 .- 1432-1084. ; 32, s. 1127-1134
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Journal article (peer-reviewed)abstract
- Objectives To assess inter-modality agreement and accuracy for medial temporal lobe atrophy (MTA) ratings across radiologists with varying clinical experience in a non-demented population. Methods Four raters (two junior radiologists and two senior neuroradiologists) rated MTA on CT and MRI scans using Scheltens' MTA scale. Ratings were compared to a consensus rating by two experienced neuroradiologists for estimation of true positive and negative rates (TPR and TNR) and over- and underestimation of MTA. Inter-modality agreement expressed as Cohen's kappa (dichotomized data), Cohen's kappa(w), and two-way mixed, single measures, consistency ICC (ordinal data) were determined. Adequate agreement was defined as kappa/kappa(w) >= 0.80 and ICC >= 0.80 (significance level at 95% CI >= 0.65). Results Forty-nine subjects (median age 72 years, 27% abnormal MTA) with cognitive impairment were included. Only junior radiologists achieved adequate agreement expressed as Cohen's kappa. All raters achieved adequate agreement expressed as Cohen's kappa(w) and ICC. True positive rates varied from 69 to 100% and TNR varied from 85 to 100%. No under- or overestimation of MTA was observed. Ratings did not differ between radiologists. Conclusion We conclude that radiologists with varying experience achieve adequate inter-modality agreement and similar accuracy when Scheltens' MTA scale is used to rate MTA on a non-demented population. However, TPR varied between radiologists which could be attributed to rating style differences.
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| 22. |
- Nazir, Madiha, et al.
(author)
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Targeting tumor cells based on Phosphodiesterase 3A expression
- 2017
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In: Experimental Cell Research. - : Elsevier BV. - 0014-4827 .- 1090-2422. ; 361:2, s. 308-315
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Journal article (peer-reviewed)abstract
- We and others have previously reported a correlation between high phosphodiesterase 3 A (PDE3A) expression and selective sensitivity to phosphodiesterase (PDE) inhibitors. This indicates that PDE3A could serve both as a drug target and a biomarker of sensitivity to PDE3 inhibition. In this report, we explored publicly available mRNA gene expression data to identify cell lines with different PDE3A expression. Cell lines with high PDE3A expression showed marked in vitro sensitivity to PDE inhibitors zardaverine and quazinone, when compared with those having low PDE3A expression. Immunofluorescence and immunohistochemical stainings were in agreement with PDE3A mRNA expression, providing suitable alternatives for biomarker analysis of clinical tissue specimens. Moreover, we here demonstrate that tumor cells from patients with ovarian carcinoma show great variability in PDE3A protein expression and that level of PDE3A expression is correlated with sensitivity to PDE inhibition. Finally, we demonstrate that PDE3A is highly expressed in subsets of patient tumor cell samples from different solid cancer diagnoses and expressed at exceptional levels in gastrointestinal stromal tumor (GIST) specimens. Importantly, vulnerability to PDE3 inhibitors has recently been associated with co-expression of PDE3A and Schlafen family member 12 (SLFN12). We here demonstrate that high expression of PDE3A in clinical specimens, at least on the mRNA level, seems to be frequently associated with high SLFIV12 expression. In conclusion, PDE3A seems to be both a promising biomarker and drug target for individualized drug treatment of various cancers.
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| 23. |
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| 24. |
- Nordlund, Michael, et al.
(author)
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Catenane dimer formation in tether-assisted trans-bis-pyrrolidination of [60]fullerene
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Other publication (other academic/artistic)abstract
- Trans-bis-pyrrolidination of [60]fullerene employing a new bis-aldehyde tether with appended octadecyloxy substituents proceeded to give the desired product as well as catenane dimers. For pyrrolidinations employing sarcosine, the products were separable, and characterisation by NMR spectroscopy, including diffusion-ordered spectroscopy (DOSY), provided unambiguous proof that the high-mass product was a symmetric non-covalent catenane. No catenane or other high-mass products were observed for reactions employing a tether without the octadecyloxy substituents.
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| 25. |
- Nordlund, Michael, et al.
(author)
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Mono- and diamides of 1,1’-dicarboxyferrocene: a stepwise approach to ferrocene-linked triads
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Other publication (other academic/artistic)abstract
- An improved route to the monomethyl ester of 1,1´-dicarboxyferrocene is described. The compound is a key intermediate for the assembly of ferrocene-bridged triads by stepwise amidation. This renders possible symmetric as well as non-symmetric diamides, exemplified by the preparaton of a symmetric ferrocene-linked fulleropyrrolidine dumbbell and a non-symmetric fulleropyrrolidine-ferrocene-tryptophan triad.
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| 26. |
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| 27. |
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| 28. |
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| 29. |
- Nordlund, Michael, et al.
(author)
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Towards [60]fullerene-based molecular wires: amide-interlinked ferrocene-[60]fullerene oligomers
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Other publication (other academic/artistic)abstract
- Design and synthesis of amide-linked ferrocene-fulleropyrrolidine oligomers is presented, with initial characterisation that confirms products containing three or four ferrocene-bridged fullerene units. Side-products have been identified; the encountered issues concerning their formation and general solubility motivated modifications of the synthesis strategy.
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| 30. |
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| 31. |
- Omerovic, Elmir, et al.
(author)
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Bivalirudin versus heparin in ST and non-ST-segment elevation myocardial infarction-Outcomes at two years
- 2024
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In: Cardiovascular Revascularization Medicine. - : Elsevier. - 1553-8389 .- 1878-0938.
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Journal article (peer-reviewed)abstract
- BACKGROUND: The registry-based randomized VALIDATE-SWEDEHEART trial (NCT02311231) compared bivalirudin vs. heparin in patients undergoing percutaneous coronary intervention (PCI) for myocardial infarction (MI). It showed no difference in the composite primary endpoint of death, MI, or major bleeding at 180 days. Here, we report outcomes at two years.METHODS: Analysis of primary and secondary endpoints at two years of follow-up was prespecified in the study protocol. We report the study results for the extended follow-up time here.RESULTS: In total, 6006 patients were enrolled, 3005 with ST-segment elevation MI (STEMI) and 3001 with Non-STEMI (NSTEMI), representing 70 % of all eligible patients with these diagnoses during the study. The primary endpoint occurred in 14.0 % (421 of 3004) in the bivalirudin group compared with 14.3 % (429 of 3002) in the heparin group (hazard ratio [HR] 0.97; 95 % confidence interval [CI], 0.85-1.11; P = 0.70) at one year and in 16.7 % (503 of 3004) compared with 17.1 % (514 of 3002), (HR 0.97; 95 % CI, 0.96-1.10; P = 0.66) at two years. The results were consistent in patients with STEMI and NSTEMI and across major subgroups.CONCLUSIONS: Until the two-year follow-up, there were no differences in endpoints between patients with MI undergoing PCI and allocated to bivalirudin compared with those allocated to heparin.REGISTRATION: URL: https://www. CLINICALTRIALS: gov; Unique identifier: NCT02311231.
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| 32. |
- Vannas, Christoffer, et al.
(author)
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Treatment Monitoring of a Patient with Synchronous Metastatic Angiosarcoma and Breast Cancer Using ctDNA
- 2024
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In: International Journal of Molecular Sciences. - 1661-6596 .- 1422-0067. ; 25:7
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Journal article (peer-reviewed)abstract
- Angiosarcoma is a rare and aggressive type of soft-tissue sarcoma with high propensity to metastasize. For patients with metastatic angiosarcoma, prognosis is dismal and treatment options are limited. To improve the outcomes, identifying patients with poor treatment response at an earlier stage is imperative, enabling alternative therapy. Consequently, there is a need for improved methods and biomarkers for treatment monitoring. Quantification of circulating tumor-DNA (ctDNA) is a promising approach for patient-specific monitoring of treatment response. In this case report, we demonstrate that quantification of ctDNA using SiMSen-Seq was successfully utilized to monitor a patient with metastatic angiosarcoma. By quantifying ctDNA levels using 25 patient-specific mutations in blood plasma throughout surgery and palliative chemotherapy, we predicted the outcome and monitored the clinical response to treatment. This was accomplished despite the additional complexity of the patient having a synchronous breast cancer. The levels of ctDNA showed a superior correlation to the clinical outcome compared with the radiological evaluations. Our data propose a promising approach for personalized biomarker analysis to monitor treatment in angiosarcomas, with potential applicability to other cancers and for patients with synchronous malignancies.
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