| 1. |
- Cossarizza, A., et al.
(författare)
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Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
- 2019
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Ingår i: European Journal of Immunology. - : Wiley. - 0014-2980 .- 1521-4141. ; 49:10, s. 1457-1973
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Tidskriftsartikel (refereegranskat)abstract
- These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.
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| 2. |
- Akiba, K., et al.
(författare)
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LHC forward physics
- 2016
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Ingår i: Journal of Physics G: Nuclear and Particle Physics. - : IOP Publishing. - 0954-3899 .- 1361-6471. ; 43:11
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Tidskriftsartikel (refereegranskat)
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| 3. |
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| 4. |
- Alfirevic, Andrej, et al.
(författare)
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Serratus anterior and pectoralis plane blocks for robotically assisted mitral valve repair : a randomised clinical trial
- 2023
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Ingår i: British Journal of Anaesthesia. - 0007-0912. ; 130:6, s. 786-794
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Tidskriftsartikel (refereegranskat)abstract
- Background: Minimally invasive cardiac surgery provokes substantial pain and therefore analgesic consumption. The effect of fascial plane blocks on analgesic efficacy and overall patient satisfaction remains unclear. We therefore tested the primary hypothesis that fascial plane blocks improve overall benefit analgesia score (OBAS) during the initial 3 days after robotically assisted mitral valve repair. Secondarily, we tested the hypotheses that blocks reduce opioid consumption and improve respiratory mechanics. Methods: Adults scheduled for robotically assisted mitral valve repairs were randomised to combined pectoralis II and serratus anterior plane blocks or to routine analgesia. The blocks were ultrasound-guided and used a mixture of plain and liposomal bupivacaine. OBAS was measured daily on postoperative Days 1–3 and were analysed with linear mixed effects modelling. Opioid consumption was assessed with a simple linear regression model and respiratory mechanics with a linear mixed model. Results: As planned, we enrolled 194 patients, with 98 assigned to blocks and 96 to routine analgesic management. There was neither time-by-treatment interaction (P=0.67) nor treatment effect on total OBAS over postoperative Days 1–3 with a median difference of 0.08 (95% confidence interval [CI]: –0.50 to 0.67; P=0.69) and an estimated ratio of geometric means of 0.98 (95% CI: 0.85–1.13; P=0.75). There was no evidence of a treatment effect on cumulative opioid consumption or respiratory mechanics. Average pain scores on each postoperative day were similarly low in both groups. Conclusions: Serratus anterior and pectoralis plane blocks did not improve postoperative analgesia, cumulative opioid consumption, or respiratory mechanics during the initial 3 days after robotically assisted mitral valve repair. Clinical trial registration: NCT03743194.
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| 5. |
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| 6. |
- Claesson, P. M., et al.
(författare)
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Association between Polyelectrolytes and Oppositely Charged Surfactants in Bulk and at Solid/Liquid Interfaces
- 2010
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Ingår i: Colloids and Interface Science Series. - Weinheim, Germany : Wiley-Blackwell. - 9783527631193 - 9783527314614 ; , s. 337-395
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Mixtures of polyelectrolytes and oppositely charged surfactants find applications in many processes and products that are used in our daily life. Such systems also show many interesting features from a scientific point of view. Due to the combined technological relevance and scientific challenge, considerable research efforts have been made in this area in recent years. This has resulted in new theoretical approaches, the development of simulation methods and new experimental techniques and, of course, a large body of new findings. Together, these efforts have increased the understanding significantly, especially during the last 5 years. This chapter reviews some aspects of this topic, focusing on bulk association and association at solid/liquid interfaces. The review is focused on developments during the 21st century, even though in some cases a historical perspective is also offered.
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| 7. |
- Claesson, Per M., et al.
(författare)
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Mixtures of cationic polyelectrolyte and anionic surfactant studied with small-angle neutron scattering
- 2000
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Ingår i: Journal of Physical Chemistry B. - : American Chemical Society (ACS). - 1089-5647 .- 1520-6106 .- 1520-5207. ; 104:49, s. 11689-11694
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Tidskriftsartikel (refereegranskat)abstract
- Small-angle neutron scattering (SANS) data for solutions containing a highly charged cationic polyelectrolyte and an anionic surfactant are presented. The scattering data were obtained in pure D2O, emphasizing the scattering from the polyelectrolyte, and in a H2O/D2O mixture that contrast matches the polyelectrolyte. In the absence of surfactant, a broad scattering peak due to the mesh size of the polyelectrolyte solution is the most characteristic feature. This peak moves to larger q-values (smaller distances) as the polyelectrolyte concentration is increased, as expected for a semidilute polyelectrolyte solution. Addition of a small amount of surfactant reduces and finally removes this peak. Instead a sharp diffraction peak appears at high q-values. This Bragg peak corresponds to a characteristic distance of 37-39 Angstrom, and it is observed when either the polyelectrolyte or the surfactant is contrast matched by the solvent. Once this peak has appeared, its position does not change when the surfactant concentration is increased. The intensity of the peak grows, however, until a stoichiometric polyelectrolyte-surfactant complex has been formed. The Bragg peak remains in excess surfactant solution. These results are discussed in relation to the structure of the polyelectrolyte-surfactant aggregates and in connection with recent results from surface force and turbidity measurements using the same polyelectrolyte-surfactant pair.
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| 8. |
- Dicaire, I, et al.
(författare)
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Hα kinematics of the Spitzer Infrared Nearby Galaxies Survey - II
- 2008
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Ingår i: Monthly Notices of the Royal Astronomical Society. - : Oxford University Press (OUP). - 0035-8711 .- 1365-2966. ; 385:2, s. 553-605
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Tidskriftsartikel (refereegranskat)abstract
- This is the second part of an Hα kinematics follow-up survey of the Spitzer Infrared Nearby Galaxies Survey (SINGS) sample. The aim of this paper is to shed new light on the role of baryons and their kinematics and on the dark/luminous matter relation in the star-forming regions of galaxies, in relation with studies at other wavelengths. The data for 37 galaxies are presented. The observations were made using Fabry-Perot interferometry with the photon-counting camera FaNTOmM on four different telescopes, namely the Canada-France-Hawaii 3.6-m, the ESO La Silla 3.6-m, the William Herschel 4.2-m and the Observatoire du mont Mégantic 1.6-m telescopes. The velocity fields are computed using custom IDL routines designed for an optimal use of the data. The kinematical parameters and rotation curves are derived using the GIPSY software. It is shown that non-circular motions associated with galactic bars affect the kinematical parameters fitting and the velocity gradient of the rotation curves. This leads to incorrect determinations of the baryonic and dark matter distributions in the mass models derived from those rotation curves.
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