| 1. |
- Antonsson, Johan, et al.
(författare)
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Diffuse reflectance spectroscopy measurements for tissue type discrimination during deep brain stimulation
- 2008
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Ingår i: Journal of neural engineering. - : IOP Publishing. - 1741-2560 .- 1741-2552. ; 5:2, s. 185-190
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Tidskriftsartikel (refereegranskat)abstract
- Diffuse reflectance spectroscopy as a method for improving intracerebral guidance during functional neurosurgery has been investigated. An optical probe was developed for measurements during stereotactic and functional neurosurgery in man. The aim of the study was to investigate the spectral differences between white and grey matter and between white matter and functional targets. Diffuse reflectance spectroscopy measurements in ten patients were recorded at incremental steps towards and in three different functional targets (STN, GPi and Zi). The recorded spectra along the trajectory were sorted into white or grey matter, based on preoperative MRI images or the recorded spectral shape and intensity. The difference between tissue types was calculated as a quotient. Significant intensity differences between white and grey matter were found to be at least 14% (p < 0.05) and 20% (p < 0.0001) for MRI and spectral-sorted data respectively. The reflectance difference between white matter and the functional targets of GPi was higher than for STN and Zi. The results indicate that diffuse reflectance spectroscopy has a potential to be developed to a suitable complement to other intracerebral guidance methods.
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| 2. |
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| 3. |
- Wårdell, Karin, et al.
(författare)
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Intracerebral microvascular measurements during deep brain stimulation implantation using laser doppler perfusion monitoring
- 2007
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Ingår i: Stereotactic and Functional Neurosurgery. - : S. Karger. - 1011-6125 .- 1423-0372. ; 85:6, s. 279-286
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the study was to investigate if laser Doppler perfusion monitoring (LDPM) can be used in order to differentiate between gray and white matter and to what extent microvascular perfusion can be recorded in the deep brain structures during stereotactic neurosurgery. An optical probe constructed to fit in the Leksell® Stereotactic System was used for measurements along the trajectory and in the targets (globus pallidus internus, subthalamic nucleus, zona incerta, thalamus) during the implantation of deep brain stimulation leads (n = 22). The total backscattered light intensity (TLI) reflecting the grayness of the tissue, and the microvascular perfusion were captured at 128 sites. Heartbeat-synchronized pulsations were found at all perfusion recordings. In 6 sites the perfusion was more than 6 times higher than the closest neighbor indicating a possible small vessel structure. TLI was significantly higher (p < 0.005) and the perfusion significantly lower (p < 0.005) in positions identified as white matter in the respective MRI batch. The measurements imply that LDPM has the potential to be used as an intracerebral guidance tool.
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| 4. |
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| 5. |
- Antonsson, Ann-Beth, et al.
(författare)
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Arbetsmiljö för anställda ibemanningsföretag
- 2014
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Arbetsmiljöarbetet och -ansvaret för anställda i bemanningsföretag är delat mellan bemanningsföretaget och det inhyrande företaget.För att förstå hur arbetsmiljöarbetet fungerar och varför det ibland inte fungerar har viintervjuat 38 arbetsskadade bemanningsanställda om deras arbetsolyckor samtföre-trädare för fyra bemanningsföretag och två kundföretagmed väl fungerande arbetsmiljöarbete.Intervjuerna speglar svaga punkter i det komplexaarbetsmiljöarbetetoch framgångsrika sätt att hanteradessa. Resultatendiskuterades vid en workshopom arbetssätt för fungerande arbetsmiljöarbete i samarbete mellan bemanningsföretag och inhyrande företag.Vid workshopen deltog representanter för aktörer, som på olika sätt är involverade i arbetsmiljöarbetet för anställda i bemanningsföretag.Ett resultat i projektet är att god och säker arbetsmiljö för konsulterna ofta går hand i hand med kunniga konsulter och god kvalitet i de tjänster som bemanningsföretagen utför.Med denna utgångspunkthar viutvecklat ett underlag för arbetsmiljöarbetet somvisar hur olika aktörer i bemanningsföretag och i inhyrande företagkan bidra till ett effektivt arbetsmiljöarbeteför inhyrda konsulter.Underlaget karaktäriseras av redundans, dvs. att arbetsmiljöarbetet ska fortsätta fungera även om någon aktör inte skulle fullgöra sina skyldigheter.Underlaget visar hur olika aktörer i bemanningsföretag och inhyrande företag kan bidra, exempelvis ledningen, chefer och arbetsledare, konsulten samt skyddsombud, samt hur dessa aktörer kan bidra inför, underoch efteranlitande av bemanningsföretag och omen arbetsskada eller ett tillbud inträffar.Viktiga delar i arbetsmiljöarbetet är policys och värderingar i bemannings-och inhyrande företag och hur dessa implementeras i det dagliga arbetet i verksamheten.En viktig fråga är var gränsen går för att bemanningsföretag ska välja att inte hyra ut konsulter på grund av risker ochbrister i arbetsmiljön.Nyckelord samt ev. anknytning till geografiskt område eller näringsgren.
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| 6. |
- Antonsson, Ann-Beth, 1956-, et al.
(författare)
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Fatal Carbon Monoxide Intoxication After AcetyleneGas Welding of Pipes
- 2013
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Ingår i: Annals of Occupational Hygiene. - : Oxford University Press. - 0003-4878 .- 1475-3162. ; 57:5, s. 662-666
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Tidskriftsartikel (refereegranskat)abstract
- Acetylene gas welding of district heating pipes can result in exposure to high concentrations of carbon monoxide. A fatal case due to intoxication is described. Measurements of carbon monoxide revealed high levels when gas welding a pipe with closed ends. This fatality and these measurements highlight a new hazard, which must be promptly prevented.
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| 7. |
- Antonsson, Ann-Beth, et al.
(författare)
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Råd och rekommendationer för bättre arbetsmiljö vid städning
- 2016
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Arbetsmiljö vid städning är komplex och innefattar betydligt fler risker än den risk för belastningsskador som oftast uppmärksammas. Under perioden 2005-2009 drev IVL ett aktionsprogram om åtgärder mot belastningsskador inom städning. Webbplatsen Allt om städ utvecklades då av IVL i samarbete med Prevent och är en välbesökt och uppskattad webbplats om arbetsmiljö vid städning. Syftet med detta projekt, som finansieras av AFA Försäkring är att ny kunskap ska tillföras till den befintliga webbplatsen Allt om städ och på det sättet utgöra en stabil bas för arbetsmiljöarbete och god arbetsmiljö inom städsektorn. Projektets mål är att bygga upp kunskap om och utveckla metoder för hur man skapar en bra arbetsmiljö vid fönsterputsning, maskinstädning samt hemservice med inriktning på hemstädning. Målet är också att bygga upp kunskap om hur man förebygger arbetsmiljöproblem och arbetsskador som beror på fallolyckor samt hot, våld och sexuella trakasserier. I rapporten redovisas en analys av dessa risker, hur vanliga de är baserat på tillgänglig statistik, samt förslag på åtgärder som kan förebygga dem. Vi har lagt stor vikt vid att förstå vilka orsaker som ökar risken och bidrar till arbetsskador, genom att använda ett MTO-perspektiv (dvs. studerat samspelet människa, teknik, organisation) och att förstå kontexten, det vill säga i vilket sammanhang som åtgärderna ska användas, eftersom kontexten har stor betydelse för hur effektiva åtgärder blir och hur de fungerar i praktiken. Vi har analyserat arbetsskadestatistik och litteraturstudier, genomfört kvalitativa intervjuer med städpersonal, skyddsombud, arbetsledare, HR-personal, kunder och representanter för arbetsmarknadens parter samt besökt arbetsplatser för att få en förståelse för problem och möjliga lösningar. Projektet visar att fallolyckor är den vanligaste typen av allvarlig olycka bland städare. Det är därför viktigt att tydliggöra vikten av att arbeta förebyggande för att minska fallolyckorna. Chefer och anställda behöver t.ex. veta hur risken för fallolyckor kan minskas. This report is only available in Swedish. English summary is available in the report.
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| 8. |
- Antonsson, Freddy, et al.
(författare)
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Optimal time for contractors to enter infrastructure projects
- 2021
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Ingår i: Procedia Computer Science. - : Elsevier BV. - 1877-0509. ; 196, s. 990-998
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Konferensbidrag (refereegranskat)abstract
- involvement (ECI) is a project delivery method where the goal is to include construction knowledge into the design phase. This is done by procuring the contractor during the design phase of a project. There are different approaches to which time the contractor is introduced. This research paper aims to investigate the optimal time for contractors to enter infrastructure projects. In order to do this, an empirical study was conducted, where interviews were held with nine representatives from an ongoing ECI project in Sweden. The studied project was procured by the Swedish Transport Administration, and the contractor entered the project at the beginning of the design phase, before a land acquisition plan had been developed. This is the first time in Sweden that a contractor has been procured this early in a road project. The findings from the interviews show that responsibility, understanding, innovation, risk management, relationship-building and implementation are the aspects that have been affected due to ECI. By analyzing and discussing the results, it was concluded that involving the contractor as early as in the studied case has been beneficial, and that involving the contractor as early as possible in infrastructure project is favorable.
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| 9. |
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| 10. |
- Antonsson, Johan, et al.
(författare)
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In-vivo reflection spectroscopy measurements in pig brain during stereotactic surgery
- 2003
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Ingår i: Biophotonics West. - : SPIE. ; , s. 242-250
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Konferensbidrag (refereegranskat)abstract
- Radiofrequency (RF) lesioning in the human brain is a commonsurgical therapy for relieving severe pain as well as formovement disorders such as Parkinsonia. During the procedure a smallelectrode is introduced by stereotactic means towards a target arealocalized by CT or MRI. An RF-current is applied throughthe electrode tip when positioned in the target area. Thetissue in the proximity of the tip is heated bythe current and finally coagulated.The overall aim of this studywas to improve the RF-technique and its ability to estimatelesion size by means of optical methods. Therefore, the opticaldifferences between white and gray matter, as well as lesionedand unlesioned tissue were investigated. Reflection spectroscopy measurements in therange of 450-800 nm were conducted on fully anesthetized pigsduring stereotactic RF-lesioning (n=6). Light from a tungsten lamp wasguided to the electrode tip through optical fibers, inserted alonga 2 mm in diameter monopolar RF-electrode. Measurements were performedin steps of 0-10 mm from the target in eachhemisphere towards the entry point of the skull. In thecentral gray of the porcine brain measurements were performed bothbefore and after the creation of a lesion. A totalof 55 spectra were collected during this study. Correlation totissue type was done using post-operative MR-images. The spectral signaturefor white and gray matter differs significantly for the entirespectral range of 450-800 nm. Pre- and post-lesioning reflection spectroscopyshowed the largest differences below 600 and above 620 nm,which implies that lasers within this wavelength range may beuseful for in-vivo measurements of tissue optical changes during RF-lesioning.
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| 11. |
- Antonsson, Johan, 1973-
(författare)
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On optical methods for intracerebral measurements during stereotactic and functional neurosurgery : Experimental studies
- 2007
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Radio frequency (RF) lesioning and deep brain stimulation (DBS) are the two prevailing surgical treatments for movement disorders within the field of stereotactic and functional neurosurgery. For RF-lesioning, a small volume of brain tissue is coagulated and knowledge of the lesion size and growth is of great importance for the safety and outcome of the procedure. This thesis deals with adapting the laser Doppler perfusion monitoring (LDPM) technique for measurements in brain tissue during RF-lesioning. The relation between LDPM signal changes and developed lesion size was investigated. LDPM measurements were evaluated both in vitro (albumin protein solution) and in vivo in the porcine brain during RF-lesioning corresponding to a bilateral thalamotomy in man. The investigated signals from the LDPI measurements can be used for following the lesioning time course and to detect if a lesion was created, both in vitro and in the animal model. For the albumin model, both the total backscattered light intensity and the perfusion signal can be used as markers for estimating the final coagulation size, while in the animal model this conclusion was not statistical verified.Independent on surgical method, RF-lesioning or DBS, intracerebral guidance is an important aspect within stereotactic and functional neurosurgery. To increase the accuracy and precision of reaching the correct target, different methods for intracerebral guidance exist, such as microelectrode recording and impedance methods. In this thesis, the possibility of developing an optical intracerebral guidance method has been investigated. Diffuse reflectance spectroscopy served as technology and all measurements were performed stereotactically in both porcine and human brain. Measurements of white and gray matter showed large differences, with higher reflectivity for white brain matter, both in porcine and in human brain. For the human measurements during DBS-implants, large differences between white matter and functional targets were found. Additionally, differences between native and lesioned porcine brain matter were detected. Both studies support the idea of using diffuse reflectance spectroscopy for developing an intracerebral guidance method.
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| 12. |
- Antonsson, Johan, et al.
(författare)
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Optical measurements during experimental stereotactic radiofrequency lesioning
- 2006
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Ingår i: Stereotactic and Functional Neurosurgery. - : S. Karger. - 1011-6125 .- 1423-0372. ; 84:2-3, s. 118-124
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this study was to evaluate in vivo a laser Doppler measurement system in porcine brain tissue during thermal lesioning. A 2-mm monopolar radiofrequency lesioning electrode was equipped with optical fibers in order to monitor the lesioning procedure. Laser Doppler and backscattered light intensity signals were measured along the electrode trajectory and during bilateral lesioning in the central gray (70, 80 and 90°C, n = 14). The time course of the coagulation process could be followed by optical recordings. Two separate groups of tissue were identified from the intensity signals. The changes in the perfusion levels in both groups displayed significant changes (p < 0.05, n = 48) at all temperature settings, while backscattered light intensity was significant for only one group at the different temperatures (p < 0.05, n = 39). These results indicate that optical measurements correlate with lesion development in vivo. The study also indicates that it is possible to follow the lesioning process intra-operatively.
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| 13. |
- Antonsson, Johan, 1973-
(författare)
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Optical measurements in brain tissue during radio frequency lesioning
- 2004
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Radio frequency (RF) lesioning is a well well-known procedure within the field of stereotactic neurosurgery, commonly used for the treatment of movement disorders. During the intervention, an RF-electrode is inserted into the deeper parts of the brain, where a small volume of malfunctioning brain tissue will be coagulated. This procedure is irreversible, as a result, the size and position of the created lesion is crucial in order to avoid unwanted side effects. In this thesis a further development of the RF-technology is presented in order to follow a lesioning process and to estimate the created lesion size. Software, electronics, fiber optics and an RF-electrode equipped with optical fibers was developed and evaluated.Diffuse reflectance spectroscopy measurements at 450-800 nm in the central gray of porcine brain have been performed with the developed RF-electrode. Measurements of white and gray brain matter showed a large reflectance difference in the investigated wavelength interval, with higher reflectivity for white brain matter. Reflectance differences between native and coagulated, white and gray brain matter were also found .In-vitro laser Doppler perfusion monitoring measurements at 633 nm have also been performed in a newly designed flow model. An albumin solution with added glass microspheres was placed on top of a revolving disc. The motion of the liquid surrounding the electrode tip simulated a perfusion situation. Changes in both laser Doppler perfusion and total backscattered light intensity signals were collected during the creation of different sized coagulations. The volume, width and length of the created coagulations are correlated to the measured signal changes in-vitro, where larger coagulations displayed larger signal changes. The laser Doppler perfusion and backscattered light intensity signals can be used to follow the lesioning time course and estimate the developed lesion size in-vitro.
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| 14. |
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| 15. |
- Antonsson, Johan, et al.
(författare)
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Radio frequency electrode system for optical lesion size estimation in functional neurosurgery
- 2005
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Ingår i: Journal of Biomedical Optics. - : SPIE - International Society for Optical Engineering. - 1083-3668 .- 1560-2281. ; 10:3, s. 1-6
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Tidskriftsartikel (refereegranskat)abstract
- Radiofrequency(RF) lesioning in the human brain is one possible surgicaltherapy for severe pain as well as movement disorders. Oneobstacle for a safer lesioning procedure is the lack ofsize monitoring. The aim of this study was to investigateif changes in laser Doppler or intensity signals could beused as markers for size estimation during experimental RF lesioning.A 2 mm in diameter monopolar RF electrode was equippedwith optical fibers and connected to a digital laser Dopplersystem. The optical RF electrode's performance was equal to astandard RF electrode with the same dimensions. An albumin solutionwith scatterers was used to evaluate the intensity and laserDoppler signal changes during lesioning at 70, 80, and 90 °C.Significant signal changes were found for these three different clotsizes, represented by the temperatures (p<0.05, n=10). The volume, width, andlength of the created coagulations were correlated to the intensitysignal changes (r=0.88, n=30, p<0.0001) and to the perfusion signalchanges (r=0.81, n=30, p<0.0001). Both static and Doppler-shifted light canbe used to follow the lesioning procedure as well asbeing used for lesion size estimation during experimental RF lesioning.
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| 16. |
- Dahlström, Örjan, et al.
(författare)
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Clustering as a data mining method in a Web-based system for thoracic surgery
- 2001
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Ingår i: Journal of the Medical Informatics Association. Symposium Supplement. - Washington : Hanley&Belfus. - 1560535369 ; , s. 888-
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Konferensbidrag (refereegranskat)abstract
- Cluster analysis is one way of data mining from large amounts of information. Being able to perform series of analyses, varying clinical criteria and requests, expected results of the clustering might be truly rewarding. Instead of having a few hypotheses prepared and tested, medical experts can be surprised by obtaining a set of hypotheses to further validate and work on.Internet technologies enable a substantial flexibility that can be taken advantage of when implementing a Web-based tool. Division of Medical Informatics together with Linkoping Heart Center of the Linkoping University is developing procedures for multivariate clustering within the Web-based AssistMe1 system.
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| 17. |
- Hansson, Bengt Göran, et al.
(författare)
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Strong Association between Infection With Human Papillomavirus and Oral and Oropharyngeal Squamous Cell Carcinoma : A Population-based Case-control Study in Southern Sweden
- 2005
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Ingår i: Acta Oto-Laryngologica. - : Informa UK Limited. - 0001-6489 .- 1651-2251. ; 125:12, s. 1337-1344
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- CONCLUSIONS: The results of this study demonstrate a strong association between infection with high-risk types of human papillomavirus (HPV) and oral and oropharyngeal squamous cell carcinoma (OOSCC), suggesting that high-risk HPV types play a key role in car-cinogenesis. The estimated proportion of OOSCC cases attributable to HPV infection was 35%. OBJECTIVE: HPV appears to have an aetiological role in OOSCC, despite the fact that the reported prevalences of HPV in both OOSCC patients and healthy individuals have varied widely. We aimed to investigate the presence and spectrum of both high- and low-risk HPVs in all consecutive cases of OOSCC in a Swedish healthcare region over a 3-year period and in population-based, matched healthy controls. MATERIAL AND METHODS: A total of 131 patients with OOSCC were studied. Samples taken from the surface of the tumour and from the tonsillar fossa using cotton-tipped swabs were investigated, together with exfoliated cells collected using a mouthwash. Tonsillar fossa and mouthwash specimens were collected in the same way from 320 matched controls. All samples were tested for HPV DNA by nested polymerase chain reaction using the primer pairs MY09/MY11 and GP5 + /GP6+, and in positive cases the HPV type was determined by DNA sequencing. RESULTS: Infection with high-risk HPV was shown to be a strong risk factor for OOSCC (OR = 63; 95% CI 14-480). Forty-seven (36%) of the cancer patients had > or =1 specimen that was positive for a high-risk HPV type (81% of which were HPV 16), while only 3 (0.94%) of the controls were positive for a high-risk HPV type. Seven (5.3%) of the cancer patients and 13 (4.1%) of the controls were positive for any of the mucosal, mucocutaneous or cutaneous low-risk HPV types.
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| 18. |
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| 19. |
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| 20. |
- Johanson, Per-Erik, et al.
(författare)
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Early Subsidence Predicts Failure of a Cemented Femoral Stem With Minor Design Changes
- 2016
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Ingår i: Clinical Orthopaedics and Related Research. - : Ovid Technologies (Wolters Kluwer Health). - 0009-921X. ; 474:10, s. 2221-2229
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Tidskriftsartikel (refereegranskat)abstract
- Radiostereometry (RSA) measurements of early micromotion can predict later failure in hip and knee prostheses. In hip implants, RSA has been particularly helpful in the evaluation of composite-beam stem designs. The Spectron EF Primary stem (Smith & Nephew, London, UK) has shown inferior performance compared with its predecessors in both clinical studies and registry reports. Early RSA studies have shown somewhat greater subsidence for the Spectron EF Primary stem compared with the earlier Spectron EF, but still within boundaries considered to be safe. Our primary research question was whether stem subsidence and rotation for this stem design measured with RSA at 2 years can predict later stem failure. A secondary question was whether high femoral stem offset and small stem sizes, both features specific to the Spectron EF Primary stem compared with its predecessors, are associated with stem failure rate. Two hundred forty-seven hips (209 patients with median age 63 years [range, 29-80 years], 65% female, and 77% primary osteoarthritis) with a valid RSA examination at 2 years were selected from four different RSA studies (totaling 279 hips in 236 patients) in our department. The studies were primarily aimed at evaluating cup fixation, bone cement, and polyethylene types. All study patients received a cemented Spectron EF Primary stem. The selected hips had complete followup until stem failure, death, or the end of the followup period. Stem failure was defined as revision of a loose femoral stem or radiological failure with significant osteolysis in Gruen zones 2 to 6. Cox regression analyses were performed to evaluate if stem subsidence and rotation after 2 years, adjusted for age, sex, stem size, standard/high stem offset, and conventional/highly crosslinked polyethylene, could predict later clinical aseptic failure of the stem. We identified 32 stem failures (27 revisions, five radiological failures) at 14 years median followup (range, 3-18 years). Ten-year stem survival was 94% (95% confidence interval [CI], 90%-96%). Stem subsidence at 2 years (adjusted hazard ratio [HR], 6.0; 95% CI, 2.5-15; p < 0.001) and retrotorsion of the stem (adjusted HR, 1.7; 95% CI, 1.1-2.5; p = 0.018) were associated with later stem failure. Further risk factors were male sex (subsidence analysis HR, 6.9; p > 0.001), use of the two smallest stem sizes (HRsize 1, 8.0; p > 0.001, HRsize 2, 1 [reference], HRsize 3+, 0.06; p = 0.035), and the high offset option (HR, 3.1; p = 0.005). Stem subsidence and retrotorsion at 2 years can predict later failure in the Spectron EF Primary stem, consistent with earlier findings on composite-beam cemented stems. Small stem size and high-offset stems comprise the main group of underperforming stems. We recommend that premarket small-scale RSA studies be performed after any design change to a THA femoral component, because even seemingly minor design changes may unexpectedly result in inferior performance.
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| 21. |
- Kircher, Albert, et al.
(författare)
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Knowledge representation forms for data mining methodologies as applied in thoracic surgery
- 2000
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Ingår i: AMIA,2000. - Philadelphia : Hanley & Belfus Inc. - 156053480X ; , s. 428-432
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Konferensbidrag (refereegranskat)abstract
- Typical ways of disseminating and using results of clinical research are scientific journals and reports. Presentation forms are condensed and comprehensible mainly to the experts following the specific topics. A vast amount of information remains unutilized due to the complex form of presenting the knowledge. Subject of this research is to explore possibilities of representation and also visualization of the results obtained using data mining methodologies. The intention is to formulate more than scientific ways to communicate facts that are of interest for the clinicians, medical students and even patients. Internet technologies as already widely established media support knowledge representation forms such as hypertext documents and structured knowledge components. The "Assist Me" decision support system for surgical treatment of cardiac patients integrates several forms of data mining and representation methodologies. We are showing a feasibility study in which scientific outcomes were forwarded to a broad group of potential users.
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| 22. |
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| 23. |
- Linton, Johan, 1966, et al.
(författare)
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Arkitekturens ordning
- 2008
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Ingår i: Psykoanalytisk Tid/Skrift. - 1650-7398.
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 24. |
- Nore, Beston F, et al.
(författare)
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Identification of phosphorylation sites within the SH3 domains of Tec family tyrosine kinases
- 2003
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Ingår i: Biochimica et Biophysica Acta. - 0006-3002 .- 1878-2434. ; 1645:2, s. 123-132
-
Tidskriftsartikel (refereegranskat)abstract
- Tec family protein tyrosine kinases (TFKs) play a central role in hematopoietic cellular signaling. Initial activation takes place through specific tyrosine phosphorylation situated in the activation loop. Further activation occurs within the SH3 domain via a transphosphorylation mechanism, which for Bruton's tyrosine kinase (Btk) affects tyrosine 223. We found that TFKs phosphorylate preferentially their own SH3 domains, but differentially phosphorylate other member family SH3 domains, whereas non-related SH3 domains are not phosphorylated. We demonstrate that SH3 domains are good and reliable substrates. We observe that transphosphorylation is selective not only for SH3 domains, but also for dual SH3SH2 domains. However, the dual domain is phosphorylated more effectively. The major phosphorylation sites were identified as conserved tyrosines, for Itk Y180 and for Bmx Y215, both sites being homologous to the Y223 site in Btk. There is, however, one exception because the Tec-SH3 domain is phosphorylated at a non-homologous site, nevertheless a conserved tyrosine, Y206. Consistent with these findings, the 3D structures for SH3 domains point out that these phosphorylated tyrosines are located on the ligand-binding surface. Because a number of Tec family kinases are coexpressed in cells, it is possible that they could regulate the activity of each other through transphosphorylation.
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| 25. |
- Ryding, Mauritz Johan, 1981, et al.
(författare)
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X-ray induced fragmentation of size-selected salt cluster-ions stored in an ion trap
- 2014
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Ingår i: RSC Advances. - 2046-2069. ; 4:88, s. 47743-47751
-
Tidskriftsartikel (refereegranskat)abstract
- A method for spectroscopic characterization of free ionic clusters and nanoparticles utilizing X-ray synchrotron radiation is presented. We demonstrate that size-selected ammonium bisulphate cluster ions, NH 4+(NH 4HSO4) n, captured in a linear ion trap, exhibit well-defined core-level absorption edges in the reconstructed fragment-ion abundance spectra. In addition to the specific photo-fragmentation pathways observed at the N1s-, O1s- and S2p-edges, dissociation also occurs as a consequence of clusters colliding with helium present as buffer gas in the ion trap. Separate off-beam experiments were conducted to establish the activation kinetics of these collision induced dissociation processes. Furthermore, it is demonstrated that the electrons released upon photoionization of background helium are too few in number to produce multiply charged cluster ions, and thereby induce fragmentation of the salt clusters, to any significant degree. The mechanisms for photon absorption and subsequent cluster fragmentation are analysed and discussed. In addition to its inherent element specificity, the method holds promise for cluster structure elucidation resulting from the sensitivity of the near edge absorption structure to the local chemical environment of the excited atom.
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| 26. |
- Wårdell, Karin, 1959-, et al.
(författare)
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A laser Doppler system for intracerebral measurements during stereotactic neurosurgery
- 2007
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Ingår i: Engineering in Medicine and Biology Society, 2007. EMBS 2007. - : IEEE. - 9781424407873 - 9781424407880 ; , s. 4083-4086
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Konferensbidrag (refereegranskat)abstract
- A laser Doppler system for intracerebral measurements during stereotactic and functional neurosurgery is presented. The system comprises a laser Doppler perfusion monitor, an optical probe adapted for the Leksellreg stereotactic system and a personal computer with software for acquisition, data analysis and presentation. The software makes it possible to present both the perfusion and the total backscattered light intensity (TLI) in real-time. During intracerebral measurements, the perfusion signal records the tissue's microcirculation whereas the TLI signal may be used to distinguish between grey and white matter. Evaluation of the system has been done during stereotactic neurosurgery in relation to implantation of deep brain stimulation electrodes. Measurements were made along trajectories towards targets in the deep brain structure as well as in pre-calculated target areas. The measurements show that the system has a potential to be used for intracerebral guidance but further evaluation of the technique is needed.
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| 29. |
- Yavari, Nazila, et al.
(författare)
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In vitro measurements of optical properties of porcine brain using a novel compact device
- 2005
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Ingår i: Medical and Biological Engineering and Computing. - : Springer. - 0140-0118 .- 1741-0444. ; 43:5, s. 658-666
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Tidskriftsartikel (refereegranskat)abstract
- Knowledge of the optical properties of tissues can be applied in numerous medical and scientific fields, including cancer diagnostics and therapy. There are many different ways of determining the optical properties of turbid media. The paper describes measurements of the optical properties of porcine brain tissue using novel instrumentation for simultaneous absorption and scattering characterisation of small turbid samples. Integrating sphere measurements are widely used as a reference method for determination of the optical properties of relatively thin turbid samples. However, this technique is associated with bulky equipment, complicated measuring techniques, interference compensation techniques and inconvenient sample handling. It is believed that the sphere for some applications can be replaced by a new, compact device, called the combined angular and spatially resolved head sensor, to measure the optical properties of thin turbid samples. The results compare very well with data obtained with an integrating sphere for well-defined samples. The instrument was shown to be accurate to within 12% for μa and 1% for μ s ′ in measurements of intralipid-ink samples. The corresponding variations of data were 17% and 2%, respectively. The reduced scattering coefficient for porcine white matter was measured to be 100 cm−1 at 633 nm, and the value for coagulated brain tissue was 65 cm−1. The corresponding absorption coefficients were 2 and 3 cm−1, respectively.
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| 30. |
- Yavari, Nazila, et al.
(författare)
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Measurements of optical properties of pig brain tissue in vitro using a novel compact device
- 2005
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Ingår i: Progress in Biomedical Optics and Imaging - Proceedings of SPIE. - : SPIE. - 1605-7422 .- 1042-4687. ; 5864, s. 1-11
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Konferensbidrag (refereegranskat)abstract
- In numerous medical and scientific fields, knowledge of the optical properties of tissues can be applied. Among many different ways of determining the optical properties of turbid media; integrating sphere measurements are widely used. However, this technique is associated with bulky equipment, complicated measuring techniques, interference compensation techniques, and inconvenient sample handling. This paper describes measurements of the optical properties of porcine brain tissue using novel instrumentation for simultaneous absorption and scattering characterization of small turbid samples. The system used measures both angularly and spatially resolved transmission and reflection and is called Combined Angular and Spatially-resolved Head (CASH) sensor. The results compare very well with data obtained with an integrating sphere for well-defined samples. The instrument was shown to be accurate to within 12 % for μa. and 1 % for μs' in measurements of intralipid-ink samples. The corresponding variations of data were 17 %, and 2 %, respectively. The reduced scattering coefficient for porcine white matter was measured to be 100 cm-1, while the value for coagulated brain tissue was 65 cm-1. The corresponding absorption coefficients were 2 and 3 cm-1, respectively. © 2005 SPIE and OSA.
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| 31. |
- Österman, Cecilia, 1971-, et al.
(författare)
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Preventing workplace violence and threats among professional cleaners : how not to be in the ‘wrong place at the wrong time’
- 2017
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Ingår i: Proceedings of the 49<sup>th</sup> Annual Nordic Ergonomics Society Conference, "Joy at work". - Lund : Lund Universitet. - 9789177531524 ; , s. 460-467
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Konferensbidrag (refereegranskat)abstract
- Professional cleaners work in a complex work environment, subjected to various physical and mental loads. This paper focuses on work related violence and threats and practical suggestions for its prevention and mitigation. By organizing and designing cleaning work in time and space it is possible to reduce the risk of cleaners being in the 'wrong place at the wrong time’. Cleaners need to have sufficient knowledge of how their work can be carried out as safely as possible; how to act when threatening or precarious situations occur, and feel confident in managerial support if they need to interrupt work in a threatening situation.
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