| 1. |
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
- Antonsson, M., et al.
(författare)
-
Lungefysioterapi ved abdominal- og thoraxkirurgi
- 2011
-
Ingår i: Fysioterapeuten. ; 9
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- I snart hundrede år har fysioterapeuter arbejdet på at mindske risikoen for postoperative lungekomplikationer hos patienter, der skal opereres i brystkassen og abdominalregionen. Klinisk erfaring viser, at lungefysioterapi er vigtig, men hvad ved vi i dag om effekten af forskellige former for behandling? Hvilke indsatsområder skal man i første omgang vælge? Forfatterne til denne artikel har udarbejdet retningslinjer for lungefysioterapi til patienter, som gennemgår abdominal- og thoraxkirurgi. Målet med arbejdet med retningslinjerne har været at udrede og sammensætte eksisterende evidens for lungefysioterapeutiske behandlingsmetoder i forbindelse med abdominal- og thoraxkirurgiske indgreb. Den samlede evidens i kombination med ekspertgruppens kommentarer har ført til anbefalinger for den kliniske behandling. Disse anbefalinger er målrettet fysioterapeuter i den kliniske praksis, som arbejder med abdominal - og thoraxkirurgiske patienter. Sigtet er, at den aktuelle og systematisk indsamlede viden vil bidrage til diskussioner på de forskellige arbejdspladser, og at anbefalingerne for behandling vil blive tilpasset de lokale forhold. Denne artikel sammenfatter retningslinjerne, som er publiceret på fysioterapiforbundets (Legitimerede Sjukgymnasters) hjemmeside under profession. De kliniske retningslinjer omfatter desuden en komplet referenceliste.
|
|
| 5. |
|
|
| 6. |
- Ericson, E., et al.
(författare)
-
Hepatic patatin-like phospholipase domain-containing 3 levels are increased in I148M risk allele carriers and correlate with NAFLD in humans
- 2022
-
Ingår i: Hepatology communications.. - : Ovid Technologies (Wolters Kluwer Health). - 2471-254X. ; 6:10, s. 2689-2701
-
Tidskriftsartikel (refereegranskat)abstract
- In nonalcoholic fatty liver disease (NAFLD) the patatin-like phospholipase domain-containing 3 (PNPLA3) rs738409 variant is a contributor. In mice, the Pnpla3 148M variant accumulates on lipid droplets and probably leads to sequestration of a lipase cofactor leading to impaired mobilization of triglycerides. To advance our understanding of the localization and abundance of PNPLA3 protein in humans, we used liver biopsies from patients with NAFLD to investigate the link to NAFLD and the PNPLA3 148M genotype. We experimentally qualified an antibody against human PNPLA3. Hepatic PNPLA3 protein fractional area and localization were determined by immunohistochemistry in biopsies from a well-characterized NAFLD cohort of 67 patients. Potential differences in hepatic PNPLA3 protein levels among patients related to degree of steatosis, lobular inflammation, ballooning, and fibrosis, and PNPLA3 I148M gene variants were assessed. Immunohistochemistry staining in biopsies from patients with NAFLD showed that hepatic PNPLA3 protein was predominantly localized to the membranes of small and large lipid droplets in hepatocytes. PNPLA3 protein levels correlated strongly with steatosis grade (p = 0.000027) and were also significantly higher in patients with lobular inflammation (p = 0.009), ballooning (p = 0.022), and significant fibrosis (stage 2-4, p = 0.014). In addition, PNPLA3 levels were higher in PNPLA3 rs738409 148M (CG, GG) risk allele carriers compared to 148I (CC) nonrisk allele carriers (p = 0.0029). Conclusion: PNPLA3 protein levels were associated with increased hepatic lipid content and disease severity in patients with NAFLD and were higher in PNPLA3 rs738409 (148M) risk allele carriers. Our hypothesis that increased hepatic levels of PNPLA3 may be part of the pathophysiological mechanism of NAFLD is supported.
|
|
| 7. |
|
|
| 8. |
- Rodin, S, et al.
(författare)
-
Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment
- 2014
-
Ingår i: Nature communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 5, s. 3195-
-
Tidskriftsartikel (refereegranskat)abstract
- Lack of robust methods for establishment and expansion of pluripotent human embryonic stem (hES) cells still hampers development of cell therapy. Laminins (LN) are a family of highly cell-type specific basement membrane proteins important for cell adhesion, differentiation, migration and phenotype stability. Here we produce and isolate a human recombinant LN-521 isoform and develop a cell culture matrix containing LN-521 and E-cadherin, which both localize to stem cell niches in vivo. This matrix allows clonal derivation, clonal survival and long-term self-renewal of hES cells under completely chemically defined and xeno-free conditions without ROCK inhibitors. Neither LN-521 nor E-cadherin alone enable clonal survival of hES cells. The LN-521/E-cadherin matrix allows hES cell line derivation from blastocyst inner cell mass and single blastomere cells without a need to destroy the embryo. This method can facilitate the generation of hES cell lines for development of different cell types for regenerative medicine purposes.
|
|
| 9. |
- Seppä, H., et al.
(författare)
-
Spatial structure of the 8200 cal yr BP event in northern Europe
- 2007
-
Ingår i: Climate of the Past. - : Copernicus GmbH. - 1814-9324 .- 1814-9332. ; 3:2, s. 225-236
-
Tidskriftsartikel (refereegranskat)abstract
- A synthesis of well-dated high-resolution pollen records suggests a spatial structure in the 8200 cal yr BP event in northern Europe. The temperate, thermophilous tree taxa, especially Corylus, Ulmus, and Alnus, decline abruptly between 8300 and 8000 cal yr BP at most sites located south of 61° N, whereas there is no clear change in pollen values at the sites located in the North-European tree-line region. Pollen-based quantitative temperature reconstructions and several other, independent palaeoclimate proxies, such as lacustrine oxygen-isotope records, reflect the same pattern, with no detectable cooling in the sub-arctic region. The observed patterns challenges the general view of the wide-spread occurrence of the 8200 cal yr BP event in the North Atlantic region. An alternative explanation is that the cooling during the 8200 cal yr BP event took place mostly during the winter and spring, and the ecosystems in the south responded sensitively to the cooling during the onset of the growing season. In contrast, in the sub-arctic area, where the vegetation was still dormant and lakes ice-covered, the cold event is not reflected in pollen-based or lake-sediment-based records.
|
|
| 10. |
|
|
| 11. |
|
|
| 12. |
- Antonsson, H, et al.
(författare)
-
VIBRATIONAL MOTION AND GEOMETRICAL STRUCTURE IN ADSORBED CO STUDIED BY CORE LEVEL PHOTOELECTRON-SPECTROSCOPY
- 1990
-
Ingår i: Journal of Electron Spectroscopy and Related Phenomena. - 0368-2048. ; 54, s. 601-613
-
Tidskriftsartikel (refereegranskat)abstract
- High resolution core level spectra from CO adsorbed on clean and hydrogen precovered Ni(100) and CO adsorbed on Cu(100) are presented. The core level binding energy is shown to be sensitive to the adsorption site. Cluster calculations reproduce the general trend of the binding energy shifts between the on top and hollow sites of CO/Ni(100). In the coadsorption system CO/H/Ni(100) three different adsorption sites have been observed with a maximum binding energy shift of 2.6 eV for the Ols level. The temperature dependence of the Cls and Ols line profiles in CO/Ni(100) has been carefully investigated. The temperature dependent broadening is due to thermal excitations of frustrated translations parallel to the surface. The spectra from CO on Cu(100) show no temperature dependence below 200K.
|
|
| 13. |
- Antonsson, M, et al.
(författare)
-
Proteolysis of the semi-hard cheese Herrgard made at different dairies. Exploratory study
- 2003
-
Ingår i: Milchwissenschaft. - 0026-3788. ; 58:3-4, s. 145-148
-
Tidskriftsartikel (refereegranskat)abstract
- The casein breakdown, peptide hydrolysis and accumulation of amino acids were studied in the semi-hard Swedish cheese, Herrgard produced at 3 dairies. Casein components were analysed by capillary electrophoresis, and peptide profiles as well as amino acid composition with reverse phase HPLC. Ten cheeses from the same vat of each of the 3 dairies were analysed; 5 cheeses each after 3 and 6 months of ripening. The peptide profiles revealed significant quantitative differences that most probably are explained by variations in composition of starter and non-starter lactic acid bacteria in cheese. The cheeses from one dairy could be separated from the other cheeses by their amino acid composition that reflected a larger amount of starter bacteria being viable for a longer time. Variation in plasmin activity between cheeses from different dairies was shown as well as a relation between plasmin activity and amount of total free amino acids released by microbial activity.
|
|
| 14. |
- Antonsson, S., et al.
(författare)
-
A comparative study of the impact of the cooking process on oxygen delignification
- 2003
-
Ingår i: Nordic Pulp & Paper Research Journal. - : Walter de Gruyter GmbH. - 0283-2631 .- 2000-0669. ; 18:4, s. 388-394
-
Tidskriftsartikel (refereegranskat)abstract
- The impact of oxygen delignification on chemical pulps of a given kappa number manufactured in different ways (using kraft, prehydrolysis kraft and magnesium sulphite cooking) has been investigated. The prehistory of the pulps proves to be a very important factor in determining the response to oxygen delignification, i.e. the degree of delignification. It is shown that this is not due to different amounts of hexenuronic acid (HexA) in the different pulps, although this is an important factor behind the high residual kappa number after oxygen delignification of birch kraft pulp. Oxygen delignification of sulphite pulps proves to be efficient even at kappa numbers significantly lower than 10. These pulps show the greatest yield loss over the oxygen delignification. It is likely that Lignin Carbohydrate Complexes (LCC) complexes play a very important role in limiting the speed of reaction of oxygen delignification. Due to the very different prehistories of the pulps investigated, it is probable that the LCC:s are native and not formed during cooking.
|
|
| 15. |
- Antonsson, S., et al.
(författare)
-
Comparison of the physical properties of hardwood and softwood pulps
- 2009
-
Ingår i: Nordic Pulp & Paper Research Journal. - 0283-2631 .- 2000-0669. ; 24:4, s. 409-414
-
Tidskriftsartikel (refereegranskat)abstract
- High mechano-sorptive creep resistance, i.e., good creep resistance in environments with changing relative humidity, is one of the key requirements for linerboards. The aim of this study was to investigate the influence of pulp types and pulp properties on the mechano-sorptive creep of kraftliner. A high-yield softwood, kraftliner pulp, and four different hardwood pulps were investigated. The physical properties of laboratory sheets were evaluated, with emphasis on the mechanosorptive creep properties. The results showed that the density increase due to increased beating significantly improved the tensile stiffness of all pulps, while its effect on the isocyclic creep stiffness was less pronounced. The hardwood pulps showed higher tensile stiffness, better mechano-sorptive creep properties, and lower hygroexpansion than the softwood pulp at a given density. However, the softwood pulp did exhibit better tensile strength and fracture toughness properties than the hardwood pulps. The results imply that hardwood pulps can be competitive with softwood pulps in kraftliners, provided that their tensile strength and fracture toughness properties can be improved by, for example, chemical means. Furthermore, the isocyclic creep stiffness correlates with the ratio of tensile stiffness to hygroexpansion, indicating that this ratio can be used for engineering estimates of the mechano-sorptive creep performance of paper materials.
|
|
| 16. |
- Berg, Staffan, et al.
(författare)
-
In Vitro and In Vivo Evaluation of 3D Printed Capsules with Pressure Triggered Release Mechanism for Oral Peptide Delivery
- 2021
-
Ingår i: Journal of Pharmaceutical Sciences. - : Elsevier BV. - 0022-3549 .- 1520-6017. ; 110:1, s. 228-238
-
Tidskriftsartikel (refereegranskat)abstract
- In this study a 3D printed capsule designed to break from the physiological pressures in the antropyloric region was evaluated for its ability to deliver the synthetic octapeptide octreotide in beagle dogs when co-formulated with the permeation enhancer sodium caprate. The pressure sensitive capsules were compared to traditional enteric coated hard gelatin capsules and enteric coated tablets. Paracetamol, which is completely absorbed in dogs, was included in the formulations and used as an absorption marker to give information about the in vivo performance of the dosage forms. The pressure sensitive capsules released drug in 50% of the dogs. In the cases where drug was released, there was no difference in octreotide bioavailability or C-max compared to the enteric coated dosage forms. When comparing all dosage forms, a correlation was seen between paracetamol C-max and octreotide bioavailability, suggesting that a high drug release rate may be beneficial for peptide absorption when delivered together with sodium caprate. (C) 2020 Published by Elsevier Inc.
|
|
| 17. |
- Ekbom, Lars B., et al.
(författare)
-
Computer simulation of solution and growth processes during the initial stage of liquid phase sintering of tungsten heavy metal
- 2005
-
Ingår i: Scandinavian journal of metallurgy. - : Wiley. - 0371-0459 .- 1600-0692. ; 34:5, s. 312-316
-
Tidskriftsartikel (refereegranskat)abstract
- In the initial stage of liquid phase sintering, particle dissolution and growth processes occur. The melting matrix penetrates the solid particle agglomerates. A fraction of the particles dissolves in the liquid matrix phase and the original powder size will initially be reduced. At the same time the agglomerates of particles are effectively separated. In a second stage larger particles grow in equilibrium with the matrix, whilst smaller, pure particles dissolve into the matrix and the mean particle size increases. When an equilibrium is reached, the solid particles start to grow in the liquid matrix phase in accordance with the ripening process. The initial stages of liquid phase sintering have been investigated by short time sintering under microgravity and are presented in a series of micrographs. Mathematical models for the 3 stages have been developed. Computer simulations of these different stages in the solution and growth processes have been used to visualise the change in particle size distribution.
|
|
| 18. |
|
|
| 19. |
- Forslund, Ola, et al.
(författare)
-
Population-based type-specific prevalence of high-risk human papillomavirus infection in middle-aged Swedish Women.
- 2002
-
Ingår i: Journal of Medical Virology. - : Wiley. - 1096-9071 .- 0146-6615. ; 66:4, s. 535-541
-
Tidskriftsartikel (refereegranskat)abstract
- Human papillomavirus (HPV) DNA testing can be used to identify women at risk of the development of cervical cancer. The cost-effectiveness of HPV screening is dependent on the type-specific HPV prevalence in the general population. The present study describes the prevalence and spectrum of high-risk HPV types found in a large real-life population-based HPV screening trial undertaken entirely within the cervical screening program offered to middle-aged Swedish women. Cervical brush samples from 6,123 women aged 32-38 years were analyzed using a general HPV primer (GP5(+)/6(+)) polymerase chain reaction-enzyme immunoassay (PCR-EIA) combined with reverse dot-blot hybridization for confirmation and HPV typing by a single assay. In this study, 6.8% (95% CI 6.2-7.5) (417/6,123) were confirmed as high-risk HPV positive. Infections with 13 different high-risk HPV types were detected, of which HPV 16 was the most prevalent type (2.1%; 128/6,123), followed by HPV 31 (1.1%; 67/6,123). Any one of the HPV types 18, 33, 35, 39, 45, 51, 52, 56, 58, 59, or 66 was detected in 3.6% (223/6,123) of the women. Infection with two, three, and five types simultaneously was identified in 32, 5, and 1 women, respectively. The combination of PCR-EIA as a screening test and reverse dot-blot hybridization as a confirmatory test, was found to be readily applicable to a real-life population-based cervical screening. The type-specific HPV prevalence found support in previous modeling studies suggesting that HPV screening may be a favorable cervical screening strategy.
|
|
| 20. |
- Hedelin, Henrik, 1975, et al.
(författare)
-
Stability in Pelvic Triple Osteotomies in Children Using Resorbable PLGA Screws for Fixation
- 2021
-
Ingår i: Journal of Pediatric Orthopaedics. - : Ovid Technologies (Wolters Kluwer Health). - 0271-6798. ; 41:9
-
Tidskriftsartikel (refereegranskat)abstract
- Background: The triple pelvic osteotomy (TPO) is a major redirectional osteotomy used to improve the acetabular coverage of the femoral head in selected pediatric patients with hip disorders. Traditionally the iliac osteotomy is stabilized by metal screws that require a second surgery for removal. Despite favorable results for both adults and children in related pelvic osteotomies, resorbable implants have not previously been used for TPOs. This study aims to suggest a novel modified TPO surgical method in children using resorbable poly lactic-co-glycolic acid (PLGA) screws for fixation and to radiographically evaluate the postoperative stability achieved by these implants in a case series. Methods: All patients (n=11) with dysplasia of the hip (2), Perthe disease (5), or Down syndrome (4), who underwent primary TPO surgery during 2013 to 2019, using resorbable PLGA screws for fixation were included. The stability of the osteotomy was evaluated in the postoperative radiograph series using the acetabular index, migration percentage, Sharp's angle and the center-edge angle. The osteotomy angle was introduced as a parameter to confirm the postoperative integrity of the achieved correction. All cases were evaluated until radiographic healing of the iliac osteotomy. Hospital notes were analyzed for complications or local reactions in relation to the implants. Results: In all studied hips, the overall achieved correction was well maintained. The mean (SD) correction achieved, from the preoperative images to the last measurable postoperative image, was a 16.7 degrees decrease for Sharp angle, a 20.9 degrees decrease in acetabular index and a 24.7 degrees increase for the center-edge angle, respectively. There were no complications related to the bioabsorption of the implants. Conclusion: The present findings suggest that the modified TPO method, using resorbable PLGA screws, provides sufficient stability and appears to be a promising alternative to traditional TPO. Avoiding implant removal is a major benefit in a pediatric population. Resorbable screws enable the surgeon to place implants with more degrees of freedom since later implant removal is not a limiting factor.
|
|
| 21. |
|
|
| 22. |
- Holst, Bjørn, et al.
(författare)
-
Molecular switch controlling expression of the mannose-specific adhesin, Msa, in Lactobacillus plantarum
- 2019
-
Ingår i: Applied and Environmental Microbiology. - 0099-2240. ; 85:10
-
Tidskriftsartikel (refereegranskat)abstract
- Some lactic acid bacteria, especially Lactobacillus spp., possess adhesive properties enabling colonization of the human gastrointestinal tract. Two probiotic Lactobacillus plantarum strains, WCSF1 and 299v, display highly different mannose-specific adhesion, with L. plantarum 299v being superior to L. plantarum WCFS1 based on a yeast agglutination assay. A straightforward correlation between the mannose adhesion capacity and domain composition of the mannose-specific adhesin (Msa) in the two strains has not been demonstrated previously. In this study, we analyzed the promoter regions upstream of the msa gene encoding a mannose-specific adhesin in these two strains. The promoter region was mapped by primer extension and DNA sequence analysis, and only a single nucleotide change was identified between the two strains. However, Northern blot analysis showed a stronger msa transcript band in 299v than in WCFS1 correlating with the different adhesion capacities. During the establishment of a high-throughput yeast agglutination assay, we isolated variants of WCFS1 that displayed a very strong mannose-specific adhesion phenotype. The region upstream of the msa gene in these variants showed an inversion of a 104-bp fragment located between two perfectly inverted repeats present in the untranslated leader region. The inversion disrupts a strong hairpin structure that otherwise most likely would terminate the msa transcript. In addition, the ribosome binding site upstream of the msa gene, which is also masked within this hairpin structure, becomes accessible upon inversion, thereby increasing the frequency of translation initiation in the variant strains. Furthermore, Northern blot analysis showed a higher abundance of the msa transcript in the variants than in the wild type, correlating with a strong-Msa phenotype.
|
|
| 23. |
- Håkansson, M., et al.
(författare)
-
The crystal structure of staphylococcal enterotoxin H : Implications for binding properties to MHC class II and TcR molecules
- 2000
-
Ingår i: Journal of Molecular Biology. - : Elsevier BV. - 0022-2836. ; 302:3, s. 527-537
-
Tidskriftsartikel (refereegranskat)abstract
- The X-ray structure of the superantigen staphylococcal enterotoxin H (SEH) has been determined at 1.69 Å resolution. In this paper we present two structures of zinc-free SEH (apoSEH) and one zinc-loaded form of SEH (ZnSEH). SEH exhibits the conventional superantigen (SAg) fold with two characteristic domains. In ZnSEH one zinc ion per SEH molecule is bound to the C-terminal β-sheet in the region implicated for major histocompatibility complex class II (MHC class II) binding in SEA, SED and SEE. Surprisingly, the zinc ion has only two ligating amino acid residues His206 and Asp208. The other ligands to the zinc ion are two water molecules. An extensive packing interaction between two symmetry-related molecules in the crystal, 834 Å2/molecule, forms a cavity that buries the zinc ions of the molecules. This dimer-like interaction is found in two crystal forms. Nevertheless, zinc-dependent dimerisation is not observed in solution, as seen in the case of SED. A unique feature of SEH as compared to other staphylococcal enterotoxins is a large negatively charged surface close to the Zn2+ site. The interaction of SEH with MHC class II is the strongest known among the staphylococcal enterotoxins. However, SEH seems to lack a SEB-like MHC class II binding site, since the side-chain properties of structurally equivalent amino acid residues in SEH and those in SEB-binding MHC class II differ dramatically. There is also a structural flexibility between the domains of SEH. The domains of two apoSEH structures are related by a 5°rotation leading to at most 3 Å difference in C(α) positions. Since the T-cell receptor probably interacts with both domains, SEH by this rotation may modulate its binding to different TcR Vβ-chains. (C) 2000 Academic Press.
|
|
| 24. |
- Hägg, Göran M, et al.
(författare)
-
Belastningar i städyrket - vilka är de och hur kan belastningen minskas?
- 2008
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- I storleksordningen 60 % av alla städare har ont varje vecka. Ytterligare i storleksordningen 20-25 % har ont ibland. Intervjuer med städare (se Bilaga 5) samt tidigare med arbetsskadade städare visar att besvären är vanliga och ofta uppkommer gradvis. Besvären märks mest efter arbetet, varför sambandet med arbetet inte alltid är tydligt. Om inget görs, kan besvären förvärras och leda till en mer bestående skada och/eller sjukfrånvaro. Rapporten beskriver belastningar vid olika arbetsmoment som förekommer vid städning samt vid städning av några vanliga lokaltyper som kontor, sjukhus och vårdcentraler, skolor och daghem, samt åtgärder som minskar belastningen. De åtgärder som krävs för att minska belastningen är: - Mer varierade arbetsuppgifter - Moderna städmetoder. Svabbning och våtmoppning är direkt olämpliga och bör användas endast i undantagsfall. - Bra städredskap, anpassade till arbetsuppgiften. - En organisation som inte överbelastar städare och som fördelar tunga arbetsuppgifter på flera. - Att städare har utbildning i och tillämpar god arbetsteknik. - Att lokalernas inredning är städvänlig. - Att lokalernas utformning är städvänlig - Att städare och deras arbetsledare och chefer uppmärksammar när någon börjar få ont och vidtar tidiga åtgärder för att minska belastningen Rapportens slutsats är att många aktörer behöver samverka för att minska städarnas belastning, bl a företag som upphandlar städtjänster, städarbetsgivare, städare, brukare av lokaler (som städas av städare), inredare och arkitekter.
|
|
| 25. |
- Junell, Anna, et al.
(författare)
-
The POU Transcription Factor Drifter/Ventral veinless Regulates Expression of Drosophila Immune Defence Genes
- 2010
-
Ingår i: Molecular and Cellular Biology. - : American Society for Microbiology. - 0270-7306 .- 1098-5549. ; 30:14, s. 3672-3684
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Innate immunity operates as a first line of defense in multicellular organisms against infections caused by different classes of microorganisms. Antimicrobial peptides (AMPs) are synthesized constitutively in barrier epithelia to protect against microbial attack and are also upregulated in response to infection. Here, we implicate Drifter/Ventral veinless (Dfr/Vvl), a class III POU domain transcription factor, in tissue-specific regulation of the innate immune defense of Drosophila. We show that Dfr/Vvl is highly expressed in a range of immunocompetent tissues, including the male ejaculatory duct, where its presence overlaps with and drives the expression of cecropin, a potent broad-spectrum AMP. Dfr/Vvl overexpression activates transcription of several AMP genes in uninfected flies in a Toll pathway- and Imd pathway-independent manner. Dfr/Vvl activates a CecA1 reporter gene both in vitro and in vivo by binding to an upstream enhancer specific for the male ejaculatory duct. Further, Dfr/Vvl and the homeodomain protein Caudal (Cad) activate transcription synergistically via this enhancer. We propose that the POU protein Dfr/Vvl acts together with other regulators in a combinatorial manner to control constitutive AMP gene expression in a gene-, tissue-, and sex-specific manner, thus promoting a first-line defense against infection in tissues that are readily exposed to pathogens.
|
|
| 26. |
|
|
| 27. |
|
|
| 28. |
|
|
| 29. |
- Ryding, Mauritz Johan, 1981, et al.
(författare)
-
X-ray induced fragmentation of size-selected salt cluster-ions stored in an ion trap
- 2014
-
Ingår i: RSC Advances. - 2046-2069. ; 4:88, s. 47743-47751
-
Tidskriftsartikel (refereegranskat)abstract
- A method for spectroscopic characterization of free ionic clusters and nanoparticles utilizing X-ray synchrotron radiation is presented. We demonstrate that size-selected ammonium bisulphate cluster ions, NH 4+(NH 4HSO4) n, captured in a linear ion trap, exhibit well-defined core-level absorption edges in the reconstructed fragment-ion abundance spectra. In addition to the specific photo-fragmentation pathways observed at the N1s-, O1s- and S2p-edges, dissociation also occurs as a consequence of clusters colliding with helium present as buffer gas in the ion trap. Separate off-beam experiments were conducted to establish the activation kinetics of these collision induced dissociation processes. Furthermore, it is demonstrated that the electrons released upon photoionization of background helium are too few in number to produce multiply charged cluster ions, and thereby induce fragmentation of the salt clusters, to any significant degree. The mechanisms for photon absorption and subsequent cluster fragmentation are analysed and discussed. In addition to its inherent element specificity, the method holds promise for cluster structure elucidation resulting from the sensitivity of the near edge absorption structure to the local chemical environment of the excited atom.
|
|
| 30. |
- Vasquez, Alejandra, et al.
(författare)
-
DNA-based classification and sequence heterogeneities in the 16S rRNA genes of Lactobacillus casei/paracasei and related species
- 2005
-
Ingår i: Systematic and Applied Microbiology. - : Elsevier BV. - 0723-2020 .- 1618-0984. ; 28:5, s. 430-441
-
Tidskriftsartikel (refereegranskat)abstract
- The sequence differences within the 16S rRNA genes of Lactobacillus casei/paracasei and related species, Lactobacillus zeae and Lactobacillus rhamnosus, were investigated. Thirty-seven strains of mostly human or cheese origin were grouped by restriction endonuclease analysis (REA) of the total chromosomal DNA and by temporal temperature gradient gel electrophoresis (TTGE) of PCR-amplified 16S rRNA gene fragments. REA verified that all strains were genomically unique and singled out three major clusters, one L. rhamnosus-cluster and two clusters containing L. paracasei strains. The groups obtained by TTGE corresponded with one exception to the REA-clusters. In the TTGE clustering all L. paracasei strains formed one general group with one TTGE-band in common, and this group was sub-divided into five subgroups due to the presence of more than one TTGE-band in four of the subgroups. The occurrence of multiple TTGE-bands was investigated by amplifying and cloning of the 16S rRNA genes from the strains showing this phenomenon, thereby 12 clones from each strain were sequenced, demonstrating polymorphisms in almost all the cases. Subjecting the clones displaying sequence variations to TTGE as well as sequencing of 16S rDNA revealed by ribotyping of the strains, verified the presence of polymorphisms within the 16S rRNA genes. The migration characteristic of amplified DNA from a single clone corresponded to a specific band in the TTGE-pattern of the strain from which the clone originated. Southern blot hybridisation with a 16S rDNA probe demonstrated the presence of at least five 16S rRNA genes in L. casei/paracasei. A higher degree of variable positions than previously reported was observed in the 16S rRNA gene fragments of the members in the complex. Sequence comparison between the 16S rRNA gene copies of L. casei (CCUG 21451(T)) and L. zeae (CCUG 35515(T)) demonstrated that the two species shared almost the same sequence in some copies while the others were more different. Our results provide one explanation for the difficulties in reaching clear-cut taxa within the L. casei/paracasei complex.
|
|
| 31. |
- Wang, Mei, et al.
(författare)
-
T-RFLP combined with principal component analysis and 16S rRNA gene sequencing: an effective strategy for comparison of fecal microbiota in infants of different ages
- 2004
-
Ingår i: Journal of Microbiological Methods. - : Elsevier BV. - 1872-8359 .- 0167-7012. ; 59:1, s. 53-69
-
Tidskriftsartikel (refereegranskat)abstract
- The fecal microbiota of two healthy Swedish infants was monitored over time by terminal restriction fragment length polymorphism (T-RFLP) analysis of amplified 16S rRNA genes. Principal component analysis (PCA) of the T-RFLP profiles revealed that the fecal flora in both infants was quite stable during breast-feeding and a major change occurred after weaning. The two infants had different sets of microbiota at all sampling time points. 16S rDNA clone libraries were constructed and the predominant terminal restriction fragments (T-RFs) were identified by comparing T-RFLP patterns in the fecal community with that of corresponding 16S rDNA clones. Sequence analysis indicated that the infants were initially colonized mostly by members of Enterobacteriaceae, Veillonella, Enterococcus, Streptococcus, Staphylococcus and Bacteroides. The members of Enterobacteriaceae and Bacteroides were predominant during breast-feeding in both infants. However, Enterobacteriaceae decreased while members of clostridia increased after weaning. T-RFLP in combination with PCA and 16S rRNA gene sequencing was shown to be an effective strategy for comparing fecal microbiota in infants and pointing out the major changes. (C) 2004 Elsevier B.V. All rights reserved.
|
|
| 32. |
|
|
| 33. |
- Xu, Yiyi, et al.
(författare)
-
Exposure, respiratory symptoms, lung function and inflammation response of road-paving asphalt workers
- 2018
-
Ingår i: Occupational and Environmental Medicine. - : BMJ. - 1351-0711 .- 1470-7926. ; 75:7, s. 494-500
-
Tidskriftsartikel (refereegranskat)abstract
- Background Controversy exists as to the health effects of exposure to asphalt and crumb rubber modified (CRM) asphalt, which contains recycled rubber tyres. Objective To assess exposures and effects on airway symptoms, lung function and inflammation biomarkers in conventional and CRM asphalt road pavers. Methods 116 conventional asphalt workers, 51 CRM asphalt workers and 100 controls were investigated. A repeated-measures analysis included 31 workers paving with both types of asphalt. Exposure to dust, nitrosamines, benzothiazole and polycyclic aromatic hydrocarbon (PAH) was measured in worksites. Self-reported symptoms, spirometry test and blood sampling were conducted prework and postwork. Symptoms were further collected during off-season for asphalt paving. Results Dust, PAHs and nitrosamine exposure was highly varied, without difference between conventional and CRM asphalt workers. Benzothiazole was higher in CRM asphalt workers (p<0.001). Higher proportions of asphalt workers than controls reported eye symptoms with onset in the current job. Decreased lung function from preworking to postworking was found in CRM asphalt workers and controls. Preworking interleukin-8 was higher in CRM asphalt workers than in the controls, followed by a decrement after 4days of working. No differences in any studied effects were found between conventional and CRM asphalt paving. Conclusion CRM asphalt workers are exposed to higher benzothiazole. Further studies are needed to identify the source of nitrosamines in conventional asphalt. Mild decrease in lung function in CRM asphalt workers and work-related eye symptoms in both asphalt workers were observed. However, our study did not find strong evidence for severe respiratory symptoms and inflammation response among asphalt workers.
|
|
| 34. |
|
|
| 35. |
- Österman, Cecilia, 1971-, et al.
(författare)
-
Preventing workplace violence and threats among professional cleaners : how not to be in the ‘wrong place at the wrong time’
- 2017
-
Ingår i: Proceedings of the 49<sup>th</sup> Annual Nordic Ergonomics Society Conference, "Joy at work". - Lund : Lund Universitet. - 9789177531524 ; , s. 460-467
-
Konferensbidrag (refereegranskat)abstract
- Professional cleaners work in a complex work environment, subjected to various physical and mental loads. This paper focuses on work related violence and threats and practical suggestions for its prevention and mitigation. By organizing and designing cleaning work in time and space it is possible to reduce the risk of cleaners being in the 'wrong place at the wrong time’. Cleaners need to have sufficient knowledge of how their work can be carried out as safely as possible; how to act when threatening or precarious situations occur, and feel confident in managerial support if they need to interrupt work in a threatening situation.
|
|
