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- Atif, Abdul Raouf, 1996-, et al.
(författare)
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Universal Biomaterial-on-Chip : a versatile platform for evaluating cellular responses on diverse biomaterial substrates
- 2024
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Ingår i: Journal of materials science. Materials in medicine. - : Springer. - 0957-4530 .- 1573-4838. ; 35
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Tidskriftsartikel (refereegranskat)abstract
- Microfluidics has emerged as a promising approach for assessing cellular behavior in vitro, providing more physiologically relevant cell culture environments with dynamic flow and shear stresses. This study introduces the Universal Biomaterial-on-Chip (UBoC) device, which enables the evaluation of cell response on diverse biomaterial substrates in a 3D-printed microfluidic device. The UBoC platform offers mechanical stimulation of the cells and monitoring of their response on diverse biomaterials, enabling qualitative and quantitative in vitro analysis both on- and off-chip. Cell adhesion and proliferation were assessed to evaluate the biocompatibility of materials with different physical properties, while mechanical stimulation was performed to investigate shear-dependent calcium signaling in pre-osteoblasts. Moreover, the applicability of the UBoC platform in creating more complex in vitro models by culturing multiple cell types was demonstrated, establishing a dynamic multicellular environment to investigate cellular interfaces and their significance in biological processes. Overall, the UBoC presents an adaptable tool for in vitro evaluation of cellular behavior, offering opportunities for studying various biomaterials and cell interactions in microfluidic environments.
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| 2. |
- Atif, Abdul Raouf, 1996-, et al.
(författare)
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Universal Biomaterial-on-Chip: A modular platform for flexible biomaterial integration and versatile quantitative assessment
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- A requirement for clinical approval is verification of a biomaterial’s functionality and biocompatibility. However, discrepancies between in vitro and in vivo evaluations have been reported, possibly due in part to a lack of physiological relevance of typical in vitro culture set-ups. We introduce a Universal Biomaterial-on-Chip (UBoC), which is a microfluidics device that allows integration of biomaterials with varied shapes and properties and subsequent evaluation of in vitro performance under design considerations that resemble physiological conditions. In addition, UBoC operates with multifunctional modalities such as continuous perfusion, shear stress mechanostimulation and cell co-culture. The device is constructed using simple 3D printing and microfabrication techniques and its cell culture area resembles a 96-well plate (0.32 cm2). Successful cell adhesion and proliferation was observed on-chip on different materials (hydroxyapatite, titanium and fibrin) using fluorescence microscopy. Furthermore, device applicability for mechanostimulation was demonstrated through shear stimulation, where sensitivity of pre-osteoblasts to flow was captured via live Ca2+ imaging. Finally, the modularity of the UBoC platform for on-chip co-culture experiments was established after simple modifications of on-board fluidic arrangements. Overall, the UBoC presents a useful tool that augments existing in vitro testing strategies and enables thorough comparisons between biomaterials in tunable culture conditions.
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| 3. |
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| 4. |
- Schmidheini, Lukas, et al.
(författare)
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Self-Assembly of Nanodiamonds and Plasmonic Nanoparticles for Nanoscopy
- 2022
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Ingår i: Biosensors. - : MDPI AG. - 2079-6374. ; 12:3
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Tidskriftsartikel (refereegranskat)abstract
- Nanodiamonds have emerged as promising agents for sensing and imaging due to their exceptional photostability and sensitivity to the local nanoscale environment. Here, we introduce a hybrid system composed of a nanodiamond containing nitrogen-vacancy center that is paired to a gold nanoparticle via DNA hybridization. Using multiphoton optical studies, we demonstrate that the harmonic mode emission generated in gold nanoparticles induces a coupled fluorescence emission in nanodiamonds. We show that the flickering of harmonic emission in gold nanoparticles directly influences the nanodiamonds' emissions, resulting in stochastic blinking. By utilizing the stochastic emission fluctuations, we present a proof-of-principle experiment to demonstrate the potential application of the hybrid system for super-resolution microscopy. The introduced system may find applications in intracellular biosensing and bioimaging due to the DNA-based coupling mechanism and also the attractive characteristics of harmonic generation, such as low power, low background and tissue transparency.
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| 5. |
- Tiefenauer, Raphael F., et al.
(författare)
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Fast and Versatile Multiscale Patterning by Combining Template-Stripping with Nanotransfer Printing
- 2018
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Ingår i: ACS Nano. - : AMER CHEMICAL SOC. - 1936-0851 .- 1936-086X. ; 12:3, s. 2514-2520
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Tidskriftsartikel (refereegranskat)abstract
- Metal nanostructures are widely used in plasmonic and electronic applications due to their inherent properties. Often, the fabrication of such nanostructures is limited to small areas, as the processing is costly, low throughput, and comprises harsh fabrication conditions. Here, we introduce a template-stripping based nanotransfer printing method to overcome these limitations. This versatile technique enables the transfer of arbitrary thin film metal structures onto a variety of substrates, including glass, Kapton, silicon, and PDMS. Structures can range from tens of nanometers to hundreds of micrometers over a wafer scale area. The process is organic solvent-free, multilayer compatible, and only takes minutes to perform. The stability of the transferred gold structures on glass exceeds by far those fabricated by e-beam evaporation. Therefore, an adhesion layer is no longer needed, enabling a faster and cheaper fabrication as well as the production of superior nanostructures. Structures can be transferred onto curved substrates, and the technique is compatible with roll-to-roll fabrication; thus, the process is suitable for flexible and stretchable electronics.
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