| 1. |
- Kjellbom, Per, et al.
(författare)
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Cytochrome P-450/420 in plant plasma membranes : a possible component of the blue-light-reducible flavoprotein-cytochrome complex
- 1985
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Ingår i: Photochemistry and Photobiology. - : Wiley. - 0031-8655 .- 1751-1097. ; 42:6, s. 779-783
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Tidskriftsartikel (refereegranskat)abstract
- Carbon monoxide difference spectra and pyridine binding spectra indicate the presence of cytochrome P-450/420 in plasma membranes from cauliflower inflorescences. Mild lithium dodecylsulfate polyacrylamide gel electrophoresis shows only one heme staining band in the plasma membrane fraction at an apparent molecular weight of 93 kiloDalton. This band is suggested to be due to a cytochrome P-450/420 dimer, in view of the known molecular weights of animal cytochromes P-450/420. The plasma membrane-bound cytochrome P-450/420 is probably identical to the blue-light-reducible b-type cytochrome of plant plasma membranes, which has been inferred to have a role in photomorphogenesis.
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| 2. |
- Almers, Ellen, 1961-, et al.
(författare)
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Children’s preferences for schoolyard features and understanding of ecosystem service innovations – a study in five Swedish preschools
- 2021
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Ingår i: Journal of Adventure Education and Outdoor Learning. - : Taylor & Francis. - 1472-9679 .- 1754-0402. ; 21:3, s. 230-246
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Tidskriftsartikel (refereegranskat)abstract
- This study was carried out within a project to promote health and ecosystem services, ?the benefits people obtain from ecosystems?, in preschools in Sweden. The paper applies the concept ?affordance? to capture the functional meaning that children assign to different material aspects of their schoolyards before and after the installation of additional environmental features. The findings from walk-and-talks with 23 preschool children highlight what features children preferred in their yards and why. Few children showed spontaneous attention to the installed features, e.g. insect hotels. This might be more because children were not enough involved within the schoolyard development and experienced little guided exploration of environmental affordances, rather than a lack of interest per se. Given this, we suggest that development projects to upgrade schoolyards for improved ecosystem services should involve children in the design of the ecosystem services promoting features throughout the development work, and thereby, integrally, promote ecological literacy.
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| 3. |
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| 4. |
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| 5. |
- Almers, Ellen, 1961-, et al.
(författare)
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The perfect schoolyard for future children : Primary school children’s participation in envisioning workshops
- 2023
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Ingår i: Children, Youth and Environments. - : University of Cincinnati. - 1546-2250. ; 33:1, s. 101-121
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Tidskriftsartikel (refereegranskat)abstract
- This Swedish study investigates how primary school children’s perspectives on a “good schoolyard” can be illuminated through envisioning workshops using model-making. In addition to advocating for the qualities and affordances of the standard schoolyard equipment, children also suggested and constructed new features affording playing with domestic animals, being in peace and quiet in tree houses, picking fruit, and using digital playgrounds.For the children to go beyond reproducing the features, environmental qualities, and affordances of their current schoolyards, they needed plenty of time for communication and hands-on activities, opportunities to relax, imagine, and receive input and inspiration from others’ experiences.
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| 6. |
- Almers, Ellen, 1961-, et al.
(författare)
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Why forest gardening for children? Swedish forest gardeneducators' ideas, purposes, and experiences
- 2018
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Ingår i: The Journal of Environmental Education. - : Taylor & Francis. - 0095-8964 .- 1940-1892. ; 49:3, s. 242-259
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Tidskriftsartikel (refereegranskat)abstract
- Utilizing forest gardens as urban settings for outdoor environmental education in Sweden is a new practice. These forest gardens combine qualities of a forest, e.g., multi-layered polyculture vegetation, with those of a school garden, such as accessibility and food production. The study explores both the perceived qualities of forest gardens in comparison to other outdoor settings and forest garden educators’ ideas, purposes, and experiences of activities in a three-year forest gardening project with primary school children. The data were collected through interviews and observations and analyzed qualitatively. Four reported ideas were to give children opportunities to: feel a sense of belonging to a whole; experience self-regulation and systemic dependence; experience that they can co-create with non-human organisms; and imagine possible transformation of places. Four pedagogical forest garden features are discussed.
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| 7. |
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| 8. |
- Askerlund, Per
(författare)
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Calmodulin-stimulated Ca2+-ATPases in the vacuolar and plasma membranes in cauliflower
- 1997
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Ingår i: Plant Physiology. - 0032-0889 .- 1532-2548. ; 114:3, s. 999-1007
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Tidskriftsartikel (refereegranskat)abstract
- The subcellular locations of Ca2+-ATPases in the membranes of cauliflower (Brassica oleracea L.) inflorescences were investigated. After continuous sucrose gradient centrifugation a 111-kD calmodulin (CaM)-stimulated and CaM-binding Ca2+-ATPase (BCA1; P. Askerlund [1996] Plant Physiol 110: 913–922; S. Malmstrom, P. Askerlund, M.G. Palmgren [1997] FEBS Lett 400: 324–328) comigrated with vacuolar membrane markers, whereas a 116-kD CaM-binding Ca2+-ATPase co-migrated with a marker for the plasma membrane. The 116-kD Ca2+-ATPase was enriched in plasma membranes obtained by aqueous two-phase partitioning, which is in agreement with a plasma membrane location of this Ca2+-ATPase. Countercurrent distribution of a low-density intracellular membrane fraction in an aqueous two-phase system resulted in the separation of the endoplasmic reticulum and vacuolar membranes. The 111-kD Ca2+-ATPase co-migrated with a vacuolar membrane marker after countercurrent distribution but not with markers for the endoplasmic reticulum. A vacuolar membrane location of the 111-kD Ca2+-ATPase was further supported by experiments with isolated vacuoles from cauliflower: (a) Immunoblotting with an antibody against the 111-kD Ca2+-ATPase showed that it was associated with the vacuoles, and (b) ATP-dependent Ca2+ uptake by the intact vacuoles was found to be CaM stimulated and partly protonophore insensitive.
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| 9. |
- Askerlund, Per, et al.
(författare)
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Cytochromes of plant plasma membranes : Characterization by absorbance difference spectrophotometry and redox titration
- 1989
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Ingår i: Physiologia Plantarum. - : Wiley. - 0031-9317 .- 1399-3054. ; 76:2, s. 123-134
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Tidskriftsartikel (refereegranskat)abstract
- The cytochrome composition of plasma membranes (PM) obtained by phase partitioning of microsomal fractions from spinach leaves (Spinacea oleracea L. cv. Medania), cauliflower inflorescences (Brassica oleracea L.), sugar beer leaves (Beta vulgaris L.) and barley (Hordeum vulgare L. cv. Kristina) roots and leaves was characterized by absorbance difference spectrophotometry at different reducing conditions at 20 and – 196°C, by redox titration, and by heme staining of polypeptide bands after lithium dodecyl sulfate polyacrylamide gel electrophoresis (LDS-PAGE). The location of the α-bands in the difference spectra and the loss of heme after treatment with LDS indicated that predominantly cytochromes of the b-type were present in all species tested. The total concentration of cytochrome was ca 0.35 nmol (mg protein)−1. The main component (ca 70% of total) was completely reduced by ascorbate and partly by NADH and had a midpoint potential of ca 150 mV. At – 196°C, ascorbate reduction revealed a symmetrical α-band at ca 557 nm with PM from spinach leaves, cauliflower and sugar beet leaves, but with barley root and leaf PM ascorbate reduction resulted in an asymmetrical α-band (shoulder at 552, maximum at 559 nm). In the dithionite-reduced minus ascorbate-reduced spectrum at –196°C a split α-band (552 + 558 nm) was seen with PM from all species. This minor component had a midpoint potential of ca – 50 mV and is probably identical to cytochrome b5, the presence of which would explain the relatively high NADH-cytochrome c reductase activities observed with plant PM. With PM from cauliflower, CO-difference spectra indicated that cytochromes P-420 and P-450 were present at concentrations up to 0.06 and 0.03 nmol (mg protein)−1, respectively. Visualization of cytochromes by heme staining after LDS-PAGE was complicated by endogenous peroxidase activity and by loss of heme during solubilisation. A presumptive b-cytochrome (heme-stained band at 94 kDa) was only detected with barley leaf PM.
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| 10. |
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| 11. |
- Askerlund, Per, et al.
(författare)
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Detection of distinct phosphorylated intermediates of Ca2+-ATPase and H+-ATPase in plasma membranes from Brassica oleracea
- 1993
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Ingår i: Plant physiology and biochemistry (Paris). - 0981-9428 .- 1873-2690. ; 31:5, s. 787-791
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Tidskriftsartikel (refereegranskat)abstract
- Two distinct phosphorylated intermediates representing the Ca2+-ATPase and H+-ATPase, respectively, were detected after phosphorylation of plasma membranes from cauliflower (Brassica oleracea L.) inflorescences with [gammaP-32]ATP and separation of polypeptides in an acidic gel. A 116 kDa polypeptide was identified as a Ca2+-ATPase by its Ca2+-dependent phosphorylation which was enhanced by La3+. A second polypeptide (105 kDa) also phosphorylated in the absence of Ca2+ and was identified as the H+-ATPase by immune blotting.
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| 12. |
- Askerlund, Per, et al.
(författare)
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Forest gardens – new opportunities for urban children to understand and develop relationships with other organisms
- 2016
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Ingår i: Urban Forestry & Urban Greening. - : Elsevier. - 1618-8667 .- 1610-8167. ; 20, s. 187-197
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Tidskriftsartikel (refereegranskat)abstract
- This case study explores a learning situation in a forest garden in Sweden. A forest garden is an edible polyculture landscape with different layers of mostly perennial vegetation. The forest garden is designed to maximize the yield of useful plants while minimizing the input of energy and resources, including human labour. Forest gardens may offer learning situations that contextualize interconnectedness and relations between organisms as well as situations that are beneficial for evaluative development (Kellert, 2002), i.e. the development of values, beliefs and moral perspectives in children.Twenty-seven seven to eight year old primary school children were followed in the first six months of a three year project in which they participated in developing a forest garden. The aim of the study is to investigate how the children reason with respect to different organisms’ dependence on and relations to each other, themselves included. Specifically:How do the children describe their own relationships with other organisms, as well as the relationships between other organisms in the forest garden?What values of nature are expressed by the children, and in relation to which situations in the forest garden?Data were collected in the form of field notes, audio and video recordings and photos from the children’s visits to the forest garden. The photos were used for stimulated recall in focus group interviews. The data were analysed using a combination of qualitative content analysis (Patton, 2002) and semi-quantitative methods.The children in the study presented a unidirectional perspective about the relationship between themselves and the organisms, especially the insects, in the forest garden. Rather than asking what these organisms can do for me/us, they pose the question: What can I/we do for the bugs/plants/ bees? The humanistic values, expressed by the children as a willingness to help other organisms (mostly insects) are in line with the explicit aims of the former curriculum for Biology to “promote care and respect for nature”. We should note that these humanistic values are no longer explicitly stated in the current curriculum. It is striking that the anthropocentric ecosystem services perspective (introduced in the current curriculum from grade 4), is so rare in the data. The children seldom mentioned the benefits for humans from insect pollination, even though this relationship is clearly stated by the pedagogues together with humanistic values. In observations, the children showed a great deal of curiosity for the natural environment (naturalistic value) as well as joy and enthusiasm about participating in the different activities that took place in the forest garden. Aesthetic values were expressed in relation to flowers, cones, berries, a snail’s shell etc.This study shows that forest gardens have the potential to be places where children can connect emotionally and cognitively to other organisms.
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| 13. |
- Askerlund, Per, 1960-, et al.
(författare)
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Growing nature connection through greening schoolyards : preschool teachers’ response to ecosystem services innovations
- 2022
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Ingår i: Education 3-13. - : Taylor & Francis. - 0300-4279 .- 1475-7575.
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Tidskriftsartikel (refereegranskat)abstract
- This article reports how Swedish teachers' aims and practices were modified by an ecosystem services development project that introduced insect hotels, bird boxes and planting to ten preschool yards. Teachers' understanding of ecosystem services, human-nature relationships and the impact of these on nature connectedness showed that their conceptualisations of human-nature relationships were shifting and complex, reflecting overlapping ideas about what schoolyard ecosystem services might mean to/for young children and how children's connection with nature might best be supported. The findings suggest creating pockets of urban nature in schoolyards is a useful strategy to unpack some of this complexity through direct experience of ecosystems encouraging interest in, concern for and understanding of our mutuality with nature.
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| 14. |
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| 15. |
- Askerlund, Per, et al.
(författare)
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Localization of donor and acceptor sites of NADH dehydrogenase activities using inside-out and right-side-out plasma membrane vesicles from plants
- 1988
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Ingår i: FEBS Letters. - : Wiley. - 0014-5793 .- 1873-3468. ; 239:1, s. 23-28
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Tidskriftsartikel (refereegranskat)abstract
- Inside-out and right-side-out plasma membrane vesicles from sugar beet (Beta vulgaris L.) leaves, prepared by aqueous two-phase partitioning, were used to localize donor and acceptor sites and to determine substrate affinities for plasma membrane-bound NADH dehydrogenase activities. NADH-ferricyanide and NADH-cytochrome c reductase activities were approx. 30% latent with inside-out vesicles and about 80% latent with right-side-out vesicles, indicating that both donor and acceptor sites for these activities are located on the cytoplasmic surface of the plasma membrane, and that a possible transplasma membrane electron transport would constitute only a minor proportion of the total activity.
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| 16. |
- Askerlund, Per
(författare)
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Modulation of an Intracellular Calmodulin-Stimulated Ca2+-Pumping ATPase in Cauliflower by Trypsin (The Use of Calcium Green-5N to Measure Ca2+ Transport in Membrane Vesicles)
- 1996
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Ingår i: Plant Physiology. - 0032-0889 .- 1532-2548. ; 110:3, s. 913-922
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Tidskriftsartikel (refereegranskat)abstract
- The effect of controlled trypsin digestion of a calmodulin-stimulated Ca2+-ATPase in low-density intracellular membranes from cauliflower (Brassica oleracea L.) inflorescences was investigated. Ca2+ uptake into vesicles was measured either continuously with the fluorescent Ca2+ indicator Calcium Green-5N or with a radio-active filter technique. Trypsin treatment of vesicles resulted in a 3-fold activation of Ca2+ uptake and loss of calmodulin sensitivity. Immunoblotting experiments with an antiserum raised against the Ca2+-ATPase showed that the trypsin activation was accompanied by a decrease in the amount of intact Ca2+-ATPase (111 kD) and by successive appearances of polypeptides of 102 and 99 to 84 kD. 125I-Calmodulin overlays showed that only the intact Ca2+-ATPase bound calmodulin. Removal of the calmodulin-binding domain (about 9 kD) was not enough to obtain full activation. Trypsin proteolysis resulted in a Ca2+ concentration necessary for half-maximal activity of 0.5 [mu]M, whereas a value of about 2 [mu]M was obtained with untreated membranes in the presence of calmodulin. Without trypsin treatment or calmodulin the activity was not saturated even at 57 [mu]M free Ca2+. The data suggest that trypsin digestion and calmodulin activate the cauliflower Ca2+-ATPase by at least partly different mechanisms.
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| 17. |
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| 18. |
- Askerlund, Per, et al.
(författare)
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NADH-Ferricyanide Reductase of Leaf Plasma Membranes : Partial Purification and Immunological Relation to Potato Tuber Microsomal NADH-Ferricyanide Reductase and Spinach Leaf NADH-Nitrate Reductase
- 1991
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Ingår i: Plant Physiology. - 0032-0889 .- 1532-2548. ; 95:1, s. 6-13
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Tidskriftsartikel (refereegranskat)abstract
- Plasma membranes obtained by two-phase partitioning of microsomal fractions from spinach (Spinacea oleracea L. cv Medania) and sugar beet leaves (Beta vulgaris L.) contained relatively high NADH-ferricyanide reductase and NADH-nitrate reductase (NR; EC 1.6.6.1) activities. Both of these activities were latent. To investigate whether these activities were due to the same enzyme, plasma membrane polypeptides were separated with SDS-PAGE and analyzed with immunoblotting methods. Antibodies raised against microsomal NADH-ferricyanide reductase (tentatively identified as NADH-cytochrome b5 reductase, EC 1.6.2.2), purified from potato (Solanum tuberosum L. cv Bintje) tuber microsomes, displayed one single band at 43 kilodaltons when reacted with spinach plasma membranes, whereas lgG produced against NR from spinach leaves gave a major band at 110 kilodaltons together with a few fainter bands of lower molecular mass. Immunoblotting analysis using inside-out and right-side-out plasma membrane vesicles strongly indicated that NR was not an integral protein but probably trapped inside the plasma membrane vesicles during homogenization. Proteins from spinach plasma membranes were solubilized with the zwitterionic detergent 3-[(3-cholamidopropyl) dimethylammonio] 1-propane-sulfonate and separated on a Mono Q anion exchange column at pH 5.6 with fast protein liquid chromatography. One major peak of NADH-ferricyanide reductase activity was found after separation. The peak fraction was enriched about 70-fold in this activity compared to the plasma membrane. When the peak fractions were analyzed with SDS-PAGE the NADH-ferricyanide reductase activity strongly correlated with a 43 kilodalton polypeptide which reacted with the antibodies against potato microsomal NADH-ferricyanide reductase. Thus, our data indicate that most, if not all, of the truly membrane-bound NADH-ferricyanide reductase activity of leaf plasma membranes is due to an enzyme very similar to potato tuber microsomal NADH-ferricyanide reductase (NADH-cytochrome b5 reductase).
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| 19. |
- Askerlund, Per, et al.
(författare)
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Reconstitution and Characterization of a Calmodulin-Stimulated Ca-Pumping ATPase Purified from Brassica oleracea L
- 1992
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Ingår i: Plant Physiology. - 0032-0889 .- 1532-2548. ; 100:4, s. 1670-1681
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Tidskriftsartikel (refereegranskat)abstract
- Purification and functional reconstitution of a calmodulin-stimulated Ca(2+)-ATPase from cauliflower (Brassica oleracea L.) is described. Activity was purified about 120-fold from a microsomal fraction using calmodulin-affinity chromatography. The purified fraction showed a polypeptide at 115 kD, which formed a phosphorylated intermediate in the presence of Ca(2+), together with a few polypeptides with lower molecular masses that were not phosphorylated. The ATPase was reconstituted into liposomes by 3-([cholamidopropyl]-dimethylammonio-)1-propanesulfonate (CHAPS) dialysis. The proteoliposomes showed ATP-dependent Ca(2+) uptake and ATPase activity, both of which were stimulated about 4-fold by calmodulin. Specific ATPase activity was about 5 mumol min(-1) (mg protein)(-1), and the Ca(2+)/ATP ratio was 0.1 to 0.5 when the ATPase was reconstituted with entrapped oxalate. The purified, reconstituted Ca(2+)-ATPase was inhibited by vanadate and erythrosin B, but not by cyclopiazonic acid and thapsigargin. Activity was supported by ATP (100%) and GTP (50%) and had a pH optimum of about 7.0. The effect of monovalent and divalent cations (including Ca(2+)) on activity is described. Assay of membranes purified by two-phase partitioning indicated that approximately 95% of the activity was associated with intracellular membranes, but only about 5% with plasma membranes. Sucrose gradient centrifugation suggests that the endoplasmic reticulum is the major cellular location of calmodulin-stimulated Ca(2+)-pumping ATPase in Brassica oleracea inflorescences.
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| 20. |
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| 21. |
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| 22. |
- Askerlund, Per, et al.
(författare)
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The Nordic forest garden : An educational opportunity for learning about ecological and emotional relationships between organisms
- 2014
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Ingår i: Education for sustainable development.
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Konferensbidrag (refereegranskat)abstract
- Ecosystem services as a perspective on ecological processes is highlighted in the new curriculum for Biology in years 4-9 in Sweden. The ecosystem service-approach implies an anthropocentric perspective in education and an unidirectional view on the purpose and value of other organisms than humans. This study is part of a larger project exploring an educational situation in which 27 seven to eight year-olds participate in creating mini-projects in a forest garden in order to strengthen ecosystem services such as pollination. A forest garden is an edible polyculture landscape with different layers of vegetation. The forest garden is designed to maximise the yield of useful plants while minimizing the input of energy and resources, human labor included (Crawford, 2009). While planning for and establishing a forest garden there is a need to adapt to specific local conditions. The ground must be prepared in a way that makes best use of the solar energy and waterflow through the area and the plants should be placed so that they promote each another. This demands reflection and knowledge about relationships between different kinds of plants but also about relationships between plants and animals. The aim of this substudy is to describe how the children perceive their own relationships to other organisms, as well as how they perceive the relationships between different other organisms. This is investigated in focus group interviews with seven to eight year-olds. Also field notes, video recordings and photos from the children's visits in the forest garden have been collected. The videos and photos have been used for stimulated re-call (Stough, 2001) in a second focus group interview. The data will be analyzed qualitatively (Patton, 2002). In addition to providing insights about the children's perceptions, the project will give examples of how a Nordic forest garden can be used in an educational context. Preliminary findings show cognitive/emotional/moral/ themes describing how children perceive relationships between organisms.
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| 23. |
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| 24. |
- Askerlund, Per, et al.
(författare)
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Transmembrane Electron Transport in Plasma Membrane Vesicles Loaded with an NADH-Generating System or Ascorbate
- 1991
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Ingår i: Plant Physiology. - 0032-0889 .- 1532-2548. ; 96:4, s. 1178-1184
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Tidskriftsartikel (refereegranskat)abstract
- Sugar beet (Beta vulgaris L.) leaf plasma membrane vesicles were loaded with an NADH-generating system (or with ascorbate) and were tested spectrophotometrically for their ability to reduce external, membrane-impermeable electron acceptors. Either alcohol dehydrogenase plus NAD+ or 100 millimolar ascorbate was included in the homogenization medium, and right-side-out (apoplastic side-out) plasma membrane vesicles were subsequently prepared using two-phase partitioning. Addition of ethanol to plasma membrane vesicles loaded with the NADH-generating system led to a production of NADH inside the vesicles which could be recorded at 340 nanometers. This system was able to reduce 2,6-dichlorophenolindophenol-3′-sulfonate (DCIP-sulfonate), a strongly hydrophilic electron acceptor. The reduction of DCIP-sulfonate was stimulated severalfold by the K+ ionophore valinomycin, included to abolish membrane potential (outside negative) generated by electrogenic transmembrane electron flow. Fe3+-chelates, such as ferricyanide and ferric citrate, as well as cytochrome c, were not reduced by vesicles loaded with the NADH-generating system. In contrast, right-side-out plasma membrane vesicles loaded with ascorbate supported the reduction of both ferric citrate and DCIP-sulfonate, suggesting that ascorbate also may serve as electron donor for transplasma membrane electron transport. Differences in substrate specificity and inhibitor sensitivity indicate that the electrons from ascorbate and NADH were channelled to external acceptors via different electron transport chains. Transplasma membrane electron transport constituted only about 10% of total plasma membrane electron transport activity, but should still be sufficient to be of physiological significance in, e.g. reduction of Fe3+ to Fe2+ for uptake.
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| 25. |
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| 26. |
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Early language development in nature : Theoretical handbook
- 2023
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Samlingsverk (redaktörskap) (övrigt vetenskapligt/konstnärligt)abstract
- This theoretical handbook draws together the theory and evidence underpinning the practical outputs of the project: the training programme and the practical handbook.It comprises four main parts:The first provides a review of relevant literature on early language development and a rationale for why language development in nature may be beneficial for young children. It summarises existing literature that looks at early language development in nature.In Part 2, our integrated formative evaluation process and its results are reported and discussed. In the final part of this section, we bring together some key points from our examination of theory and research to support the value of early language development in nature, summarising the new learning that has been achieved through the ELaDiNa project.The third part sets out a generic model that has been developed from what is currently known about this field, (Part 1), what we learnt from the ElaDiNa Project and its evaluation (Part 2) and from previous experience of a pilot project conducted in Marburg, Germany that formed the initial seed from which our ElaDiNa project grew.In concluding this theoretical handbook in Part 4, we summarise the successes and challenges of the project, its main learning points and suggest some implications for policy, practice and further research.
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| 27. |
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| 28. |
- Fredlund, Kenneth M., et al.
(författare)
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Comparison of the Stereospecificity and Immunoreactivity of NADH-Ferricyanide Reductases in Plant Membranes
- 1994
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Ingår i: Plant Physiology. - 0032-0889 .- 1532-2548. ; 106:3, s. 1103-1106
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Tidskriftsartikel (refereegranskat)abstract
- The substrate stereospecificity of NADH-ferricyanide reductase activities in the inner mitochondrial membrane and peroxisomal membrane of potato (Solanum tuberosum L.) tubers, spinach (Spinacea oleracea L.) leaf plasma membrane, and red beetroot (Beta vulgaris L.) tonoplast were all specific for the [beta]-hydrogen of NADH, whereas the reductases in wheat root (Triticum aestivum L.) endoplasmic reticulum and potato tuber outer mitochondrial membrane were both [alpha]-hydrogen specific. In all isolated membrane fractions one or several polypeptides with an apparent size of 45 to 55 kD cross-reacted with antibodies raised against a microsomal NADH-ferricyanide reductase on western blots.
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| 29. |
- Fredlund, K.M, et al.
(författare)
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NADH-ferricyanide reductases in plant membranes
- 1996
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Ingår i: Plant Membrane Biology- Proceedings of the Phytochemical Society of Europe -38. ; , s. 143-151
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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| 30. |
- Gustafsson, Mikael, et al.
(författare)
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Samverkan och urvalsprocess angående åtgärder med syfte att utveckla ekosystemtjänster på förskolegården
- 2019
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Region Jönköpings län inledde 2016 tillsammans med JU, Riksbyggen, Vätterhem och nio förskolor i regionen ett forskningsbaserat samverkansprojekt för att utveckla multifunktionella urbana förskolegårdar med förstärkta ekosystemtjänster för hälsa, lek, lärande och hållbarhet.Ekosystemtjänster är ett sätt att benämna produkter och tjänster som naturen förser oss med, såsom försörjande ekosystemtjänster, som ger oss frukter, bär och ätbara blad; stödjande ekosystemtjänster, som bidrar med skydd mot UV-ljus, förbättrad luftkvalitet, vattenreglering och kolbindning samt kulturella ekosystemtjänster, såsom, rekreation, välbefinnande, möjligheter till lek, rörelse och lärande, vilka bidrar till gemenskap i närmiljön.Ekosystemtjänstperspektivet har använts både vid planering, utveckling och utvärdering av multifunktionella utomhusmiljöer vid förskolorna i ett systematiskt kvalitets- och utvecklingsarbete. De 9 deltagande förskolorna utformade sina visioner och mål för sina utemiljöer i varsin workshop. Detta skedde i samverkan med forskare från SER-gruppen vid JU, av förskolorna specifikt inbjudna gäster samt en hållbarhetschef vid Riksbyggen som ledde samtliga workshops via länk.I respektive workshop inventerades och dokumenterades 15 befintliga ekosystemtjänster i Riksbyggens digitala mätverktyg för ekosystemtjänster. Deltagande förskollärare beskrev också sin vision för hur de vill att förskolegården ska utvecklas. Även denna fördes in i Riksbyggens digitala verktyg. Forskarna och Riksbyggen har förutom att dokumentera processen också funnits med som resurs genom att komma med förslag till möjliga åtgärder.Förutom att varje förskola har haft en egen workshop om sin specifika förskolegård har de tillsammans med de övriga medverkande parterna deltagit i gemensamma föreläsningar och erfarenhetsutbyten vid inspirationsträffar arrangerade av regionens folkhälsoavdelning.Ett syfte med forskningsstudien har varit att undersöka vilka ekosystemtjänster som prioriteras och utvecklas av deltagarna under såväl som efter förbättringsarbetet, samt hur urvalet av ekosystemtjänster gått till.Förskolornas workshops spelades in under våren 2017 och har analyserats kvalitativt med utgångspunkt från deltagarnas utsagor, som framkom under nämnda workshops. I dessa workshops deltog lärarna från respektive förskola, Riksbyggens representant samt projektets forskare från HLK. Efter ett och ett halvt år fick förskolorna dessutom besvara en enkät om vilka åtgärder för att stärka ekosystemtjänster som genomförts.Preliminärt visar resultaten, i flera fall, på likheter i de val av ekosystemtjänster som förskolorna valde att lyfta fram, men även att idéen till att satsa på olika ekosystemtjänster kan komma från olika kategorier av workshopdeltagare. Lärarna lyfter vid flera fall fram verksamhetens förutsättningar som grund för val av åtgärd. De åtgärder som flest förskolor valt att genomföra, enligt enkäten, var att anlägga insektshotell, plantera blommande växter för pollinerare, odla i pallkragar och sätta upp fågelholkar. Åtgärder som genomfördes av få förskolor var exempelvis utplacering av död ved och anläggning av vattenspegel. Vid den aktuella tillämpningen av Riksbyggens mätverktyg är en svaghet att ekosystemtjänster inte kvantifieras i tillräcklig grad.
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| 33. |
- Hammarsten, Maria, et al.
(författare)
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Developing ecological literacy in a forest garden : children’s perspectives
- 2019
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Ingår i: Journal of Adventure Education and Outdoor Learning. - : Taylor & Francis Group. - 1472-9679 .- 1754-0402. ; 19:3, s. 227-241
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Tidskriftsartikel (refereegranskat)abstract
- Today, cities become more dense, green spaces disappear and children spend less time outdoors. Research suggests that these conditions create health problems and lack of ecological literacy. To reverse such trends, localities are creating urban green spaces for children to visit during school time. Drawing on ideas in ecological literacy, this study investigates school children’s perspectives on a forest garden, a type of outdoor educational setting previously only scarcely researched. Data were collected through walk-and-talk conversations and informal interviews with 28 children aged 7 to 9. Many children in the study expressed strong positive feelings about the forest garden, the organized and spontaneous activities there, and caring for the organisms living there. We observed three aspects of learning in the data, potentially beneficial for the development of children’s ecological literacy: practical competence, learning how to co-exist and care, and biological knowledge and ecological understanding.
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| 34. |
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| 35. |
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| 36. |
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| 37. |
- Kristensen, Brian K., et al.
(författare)
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Identification of oxidised proteins in the matrix of rice leaf mitochondria by immunoprecipitation and two-dimensional liquid chromatography-tandem mass spectrometry
- 2004
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Ingår i: Phytochemistry. - : Elsevier BV. - 0031-9422 .- 1873-3700. ; 65:12, s. 1839-1851
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Tidskriftsartikel (refereegranskat)abstract
- Highly purified mitochondria were isolated from green 7-day-old rice leaves. The mitochondria were sonicated and the matrix fraction isolated as the 100,000g supernatant. Part of the matrix fraction was left untreated while the other part was subjected to a mild oxidative treatment (0.5 mM H2O2 + 0.2 mM CuSO4 for 10 min at room temperature). The oxidised proteins in both samples were tagged with dinitrophenylhydrazine (DNP), which forms a covalent bond with carbonyl groups. The DNP-tagged proteins were immunoprecipitated using anti-DNP antibodies and digested with trypsin. The mixture of peptides was analysed by nano-HPLC coupled online to an ESI-Quad-TOF mass spectrometer. The peptides were separated by stepwise ion exchange chromatography followed by reverse phase chromatography (2D-LC), and analysed by MS/MS. Proteins were identified by un-interpreted fragment ion database searches. Using this approach we identified 20 oxidised proteins in the control sample and a further 32 in the oxidised sample. Western blots of 2D-gels of the same samples prior to immunoprecipitation verified that the oxidation treatment increases protein oxidation also for specific proteins. Likewise Western blots showed that neither the isolation of mitochondria nor their subfractionation introduced carbonyl groups. We therefore conclude that a number of proteins are oxidised in the matrix of rice leaf mitochondria in vivo and further identify a group of proteins that are particularly susceptible to mild oxidation in vitro.
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| 38. |
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| 39. |
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| 40. |
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| 41. |
- Malmström, Susanna, et al.
(författare)
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A calmodulin-stimulated Ca2+-ATPase from plant vacuolar membranes with a putative regulatory domain at its N-terminus
- 1997
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Ingår i: FEBS Letters. - 0014-5793 .- 1873-3468. ; 400:3, s. 324-328
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Tidskriftsartikel (refereegranskat)abstract
- A cDNA, BCA1, encoding a calmodulin-stimulated Ca2+-ATPase in the vacuolar membrane of cauliflower (Brassica oleracea) was isolated based on the sequence of tryptic peptides derived from the purified protein. The BCA1 cDNA shares sequence identity with animal plasma membrane Ca2+-ATPases and Arabidopsis thaliana ACA1, that encodes a putative Ca2+ pump in the chloroplast envelope. In contrast to the plasma membrane Ca2+-ATPases of animal cells, which have a calmodulin-binding domain situated in the carboxy-terminal end of the molecule, the calmodulin-binding domain of BCA1 is situated at the amino terminus of the enzyme.
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| 42. |
- Malmström, Susanna, et al.
(författare)
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Regulatory role of the N terminus of the vacuolar calcium-ATPase in cauliflower
- 2000
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Ingår i: Plant Physiology. - : Oxford University Press (OUP). - 0032-0889 .- 1532-2548. ; 122:2, s. 517-526
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Tidskriftsartikel (refereegranskat)abstract
- The vacuolar calmodulin (CaM)-stimulated Ca2+-ATPase, BCA1p, in cauliflower (Brassica oleracea) has an extended N terminus, which was suggested to contain a CaM-binding domain (S. Malmstrom, P. Askerlund, M.G. Palmgren [1997] FEBS Lett 400: 324328). The goal of the present study was to determine the role of the N terminus in regulating BCA1p. Western analysis using three different antisera showed that the N terminus of BCA1p is cleaved off by trypsin and that the N terminus contains the CaM-binding domain. Furthermore, the expressed N terminus binds CaM in a Ca2+dependent manner. A synthetic peptide corresponding to the CaM-binding domain of BCA1p (Ala-19 to Leu-43) strongly inhibited ATP-dependent Ca2+ pumping by BCA1p in cauliflower low-density membranes, indicating that the CaM-binding region of BCA1p also has an autoinhibitory function. The expressed N terminus of BCA1p and a synthetic peptide (Ala-19 to Met-39) were good substrates for phosphorylation by protein kinase C. Sequencing of the phosphorylated fusion protein and peptide suggested serine-16 and/or serine-28 as likely targets for phosphorylation. Phosphorylation of serine-28 had no effect on CaM binding to the alanine-19 to methionine-39 peptide. Our results demonstrate the regulatory importance of the N terminus of BCA1p as a target for CaM binding, trypsin cleavage, and phosphorylation, as well as its importance as an autoinhibitory domain.
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| 43. |
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| 44. |
- Møller, I.M, et al.
(författare)
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Redox components in the plant plasma membrane
- 1988
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Ingår i: Plasma membrane oxidoreductases in control of animal and plant growth. ; , s. 57-69
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)
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| 45. |
- Palmgren, Michael Gjedde, et al.
(författare)
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Sealed Inside-Out and Right-Side-Out Plasma Membrane Vesicles : Optimal Conditions for Formation and Separation
- 1990
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Ingår i: Plant Physiology. - 0032-0889 .- 1532-2548. ; 92:4, s. 871-880
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Tidskriftsartikel (refereegranskat)abstract
- Plasma membrane preparations of high purity (about 95%) are easily obtained by partitioning in aqueous polymer two-phase systems. These preparations, however, mainly contain sealed right-side-out (apoplastic side out) vesicles. Part of these vesicles have been turned inside-out by freezing and thawing, and sealed inside-out and right-side-out vesicles subsequently separated by repeating the phase partition step. Increasing the KCI concentration in the freeze/thaw medium as well as increasing the number of freeze/thaw cycles significantly increased the yield of inside-out vesicles. At optimal conditions, 15 to 25% of total plasma membrane protein was recovered as inside-out vesicles, corresponding to 5 to 10 milligrams of protein from 500 grams of sugar beet (Beta vulgaris L.) leaves. Based on enzyme latency, trypsin inhibition of NADH-cytochrome c reductase, and H+ pumping capacity, a cross-contamination of about 20% between the two fractions of oppositely oriented vesicles was estimated. Thus, preparations containing about 80% inside-out and 80% right-side-out vesicles, respectively, were obtained. ATPase activity and H+ pumping were both completely inhibited by vanadate (Ki ≈ 10 micromolar), indicating that the fractions were completely free from nonplasma membrane ATPases. Furthermore, the polypeptide patterns of the two fractions were close to identical, which shows that the vesicles differed in sidedness only. Thus, preparations of both inside-out and right-side-out plasma membrane vesicles are now available. This permits studies on transport, signal transduction mechanisms, enzyme topology, etc., using plasma membrane vesicles of either orientation.
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| 46. |
- Richardson, Tanya, et al.
(författare)
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How does nature support early language learning? : A systematic literature review
- 2023
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Ingår i: Early years. - : Taylor & Francis. - 0957-5146 .- 1472-4421.
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Forskningsöversikt (refereegranskat)abstract
- The way a young child uses language has an impact on their future life. Early language acquisition is a determinant in adult employment, mental health and relationships with others. At the same time there is a broad evidence base that play and learning in the natural environment is beneficial for young children's physical, emotional, social and cognitive development. However, literature about how these two contributions to children's early development intersect and combine, in particular whether and how early language learning in children aged between 3 and 7 years might be enhanced in nature, is harder to find. For this paper, we undertook a systematic literature review to explore and report on research within this important area. Based on an in-depth study of 181 articles, we found that scant literature exists about how children's language is developed within natural environments. Although this appears to be a topic that is discussed in practice-oriented publications, it was found that very few researchers are focusing on and reporting within this area. Twelve papers were thoroughly analysed and three themes identified and discussed; desire to communicate, communication skills and literacy skills. This paper concludes by suggesting areas for future research.
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| 47. |
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| 48. |
- Stagell, Ulrica, 1970-, et al.
(författare)
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Action Competence - a private matter?
- 2014
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Konferensbidrag (refereegranskat)abstract
- Research on environmental education and education for sustainable development is an expanding field in educational research in Europe, as well as worldwide. With EERA celebrating 20 year, and the Decade for Education on Sustainable Development (DESD) in its final year, it is possible to make a halt for discussions of the present with perspectives of both the past and the future. Based on empirical findings on Eco-School teachers’ and instructors’ views on the appropriateness of including different sustainability-promoting actions in their teaching practices, this paper will discuss actions in relation to the Action Competence perspective, and tensions that manifests in educational settings between educational ideals of socialisation and educational ideas of liberal autonomy (Bildung), as well as, what is seen as private matter and what it is seen as part of the educational commission.From the perspective of Action Competence, and the educational ideal of Bildung, it has been claimed, as one of the dominating standpoints in the academic ESD-debate concerning the purpose of sustainability-promoting actions in education, that the actions are there, not to solve the unsustainability problems in the world, but to educate the students. The importance in ESD of learning critical decision taking in choices of actions as a citizen in a democratic society, nationally and globally has been stressed. The study is based on the premise that the inclusion of actions in teaching practices depends on teachers’ choices of lecturing, which to some degree is in turn dependent on their experience during teacher training, and explores Eco-School teachers’ and instructors’ views on the appropriateness of including different sustainability-promoting actions in teaching. As the importance of action for sustainability is highlighted in the Eco-Schools’ curricula, there are incitements for teachers at Eco-Schools to consider the inclusion of different sustainability-promoting actions in their teaching, either in terms of information and discussions about possible actions or in terms of student participation in actions.However, critique has been raised from the perspective of the need for education not to neglect the urgency of solving sustainability issues. Also, the Action Competence perspective, although based on a Bildung perspective, has been criticised for being just another form of socialisation. In addition to this, the liberal ideal of autonomy has been questioned.This paper will discuss how Eco-School teachers and instructors relate to different forms of actions, indirect/direct and individual/collective, that may take part in the private or the public sphere and be seen as personal or political. In relation to this, the Action Competence perspective will be discussed in perspectives of the present, past and future and the tensions that manifests in educational settings between educational ideals of socialisation and educational ideals of liberal autonomy (Bildung), as well as, what is seen as private matter and what it is seen as part of the educational commission.MethodInterviews with 24 Eco-School teachers at a dozen schools in southern Sweden, from pre-school to upper secondary school, and 9 Eco-School instructors employed by the Green Flag organisation at different locations in Sweden, were conducted based on a semi-structured interview protocol. Informants were asked to grade from 1-6 the appropriateness of 16 different sustainability-promoting actions to include in their teaching practices. The actions were chosen to grasp a diversity in types of actions, as well as motives for preferences for specific actions in teaching. More than 20 hours of interviews were transcribed verbatim, and were analysed with a mixed method approach including both quantitative and qualitative analyses. Mathematical and statistical analyses were made in Excel and SPSS for the groups of teachers and instructors respectively, and as a comparison between the two groups. A qualitative content analysis was made (in Nvivo) on the informants reasoning when justifying their ratings of each of the sustainability-promoting actions from the question: ‘What do the teachers refer to when they justify their positions?Expected OutcomesPreliminary results show that different direct actions, and actions which take place in the private sphere were viewed by Eco-School teachers and instructors as the most appropriate actions to include in teaching practices. However, actions related to individuals as consumers were seen by teachers as less appropriate than by the instructors, and have consequently been included less in teaching practices. The actions that were deemed least appropriate by both groups were two indirect actions occurring in the public sphere and aiming at solutions on a structural level, engaging with political parties and engaging with NGOs. They did not seem to have a repertoire of teaching approaches that would allow them to work with issues where social norms diverge strongly. This paper will discuss how Eco-School teachers and instructors relate to different forms of actions, indirect/direct and individual/collective, that may take part in the private or the public sphere and be seen as personal or political. In relation to this, the Action Competence perspective will be discussed in perspectives of the present, past and future and the tensions that manifests in educational settings between educational ideals of socialisation and educational ideas of liberal autonomy (Bildung), as well as, what is seen as private matter and what it is seen as part of the educational commission.
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| 49. |
- Stagell, Ulrica, 1970-, et al.
(författare)
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ESD and citizenship in the private and public sphere : Eco-School teachers' and instructors' views on actions as teaching content in ESD
- 2014
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Konferensbidrag (refereegranskat)abstract
- The Nordic countries, although different, have strong traditions of democratic education as collective solutions of the upbringing of individual citizens. ESD as a multinational response to problems of global concerns which aim at educating citizens capable of facing challenges of today and tomorrow, embody a tension between education as fostering into common values and education as liberation. The Action competence perspective manifest this tension in the division of different acting as behaviours or actions. With the premise that actions are an essential part of ESD aiming at developing students' action competence, this study seeks to explore actions as teaching content in ESD and examines Swedish Eco-School teachers' and instructors' views on the appropriateness of including different sustainability-promoting actions in their teaching practices. The position of teachers and instructors as potential obstacles for the inclusion of different action alternatives for sustainability in education, makes it relevant to ask questions about their views on including different actions in teaching practice.Different views were explored in interviews with 24 Eco-school teachers and 9 instructors, in which they were asked to clarify their positions on the appropriateness of including different sustainability-promoting actions in their teaching practices. The reasoning were analysed qualitatively in a content analysis. Preliminary results show differences in teachers' and instructors' views. Where teachers tended to explain potentially controversial direct actions in the private sphere as inappropriate with references to the question of privacy of students and parents, instructors tended to view the same actions as starting-points for desired discussions in the educational situation. Also, instructors to a higher degree motivated the appropriateness by the different actions' importance for sustainability.The discussion concerns teaching that addresses the individual's moral responsibility in the private sphere at the same time different action strategies for the democratic change of social structures tend to be excluded.
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| 50. |
- Stagell, Ulrica, 1970-, et al.
(författare)
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What Kind of Actions are Appropriate? Eco-School Teachers' and Instructors' Ranking of Sustainability-Promoting Actions as Content in Education for Sustainable Development (ESD)
- 2014
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Ingår i: International Electronic Journal of Environmental Education. - : International Electronic Journal of Environmental Education. - 2146-0329. ; 4:2, s. 97-113
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Tidskriftsartikel (refereegranskat)abstract
- Based on the consideration that learning about different action alternatives and strategies are essential parts of ESD, this quantitative study focuses Eco-School teachers’ and instructors’ views on including different sustainability-promoting actions in teaching practices. Direct actions, and actions that take place in the private sphere were viewed by both groups as the most appropriate actions to include in teaching practices. However, actions related to individuals as consumers were seen as less appropriate by teachers than by instructors, and consequently have been less included in teaching practices. The actions considered least appropriate by both groups were two indirect actions occurring in the public sphere aiming at solutions on a structural level, engaging with political parties and engaging with NGOs. The results highlight teaching that addresses the individual’s moral responsibility in the private sphere, and that different action strategies for the democratic change of social structures tend to be excluded.
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