| 1. |
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| 2. |
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| 3. |
- Aspegren, H., et al.
(författare)
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The Urban Water System - a Future Swedish Perspective
- 1997
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Ingår i: Water Science and Technology. - 0273-1223. ; 35:9, s. 33-44
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Tidskriftsartikel (refereegranskat)abstract
- It is argued, that the use of water can no longer be regarded as an almost free commodity. The idea to assess and value the environmental impact of the water use represents a true change of paradigm. The key issue is that any future wastewater treatment system has to be evaluated according to a quantitative criterion. This has to consider: •hygienic aspects: we believe that nobody will accept a lower hygienic standard than today. •environmental impact: the wastewater impact on the environment has steadily increased the need for better treatment. We need to be prepared for changes in the society by having a readiness manifested as continuing dynamic research environment in this area. Too often the knowledge build-up has been re-active instead of pro-active. •economising resources: wastewater treatment looks and probably should look quite different in densely populated urban areas and in rural areas. Considering resource handling one has to consider already invested capital costs, land use, water re-use, organic and nutrient use and re-use, as well as overall use of energy. •user aspects: technical functionality, economy and liability in different aspects require profound consideration. A sustainable wastewater treatment has to adapt to a local environment and the total resource demand has to be calculated, including a direct environmental impact on receiving waters, air and soil as well as energy consumption and nutrient recycling. Thus, there is an apparent need for. •quantitative performance index: the quest for sustainable development has to be based on objective reasons as well as subjective ones. An integrated performance index is part of a necessary decision making model for the design and operation of wastewater treatment systems. In this index, it is evident that a trade-off should be made between the pursued quality of the process outputs (liquid, solids, gas) and the associated efforts (investments, operation) required to achieve this considering the inputs (wastewater). In order to make this trade-off, however, a common framework is needed to quantitatively compare the different objectives. A metric to judge the sustainability of different options will facilitate a fruitful dialogue between e.g. politicians, ecologists, engineers and economists. Only a truly interdisciplinary approach can help to solve the challenges ahead.
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| 4. |
- Asplund, Annika, 1979-, et al.
(författare)
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One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells
- 2016
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Ingår i: Stem Cell Reviews and Reports. - : Springer Science and Business Media LLC. - 1550-8943 .- 1558-6804. ; 12:1, s. 90-104
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Tidskriftsartikel (refereegranskat)abstract
- Human hepatocytes display substantial functional inter-individual variation regarding drug metabolizing functions. In order to investigate if this diversity is mirrored in hepatocytes derived from different human pluripotent stem cell (hPSC) lines, we evaluated 25 hPSC lines originating from 24 different donors for hepatic differentiation and functionality. Homogenous hepatocyte cultures could be derived from all hPSC lines using one standardized differentiation procedure. To the best of our knowledge this is the first report of a standardized hepatic differentiation procedure that is generally applicable across a large panel of hPSC lines without any adaptations to individual lines. Importantly, with regard to functional aspects, such as Cytochrome P450 activities, we observed that hepatocytes derived from different hPSC lines displayed inter-individual variation characteristic for primary hepatocytes obtained from different donors, while these activities were highly reproducible between repeated experiments using the same line. Taken together, these data demonstrate the emerging possibility to compile panels of hPSC-derived hepatocytes of particular phenotypes/genotypes relevant for drug metabolism and toxicity studies. Moreover, these findings are of significance for applications within the regenerative medicine field, since our stringent differentiation procedure allows the derivation of homogenous hepatocyte cultures from multiple donors which is a prerequisite for the realization of future personalized stem cell based therapies.
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| 5. |
- Davidsson, Åsa, et al.
(författare)
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Co-digestion of grease trap sludge and sewage sludge.
- 2008
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Ingår i: Waste Management: international journal of integrated waste management, science and technology. - : Elsevier BV. - 1879-2456. ; 28:6, s. 986-992
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Tidskriftsartikel (refereegranskat)abstract
- Redirection of organic waste, from landfilling or incineration, to biological treatment such as anaerobic digestion is of current interest in the Malmö-Copenhagen region. One type of waste that is expected to be suitable for anaerobic digestion is sludge from grease traps. Separate anaerobic digestion of this waste type and co-digestion with sewage sludge were evaluated. The methane potential was measured in batch laboratory tests, and the methane yield was determined in continuous pilot-scale digestion. Co-digestion of sludge from grease traps and sewage sludge was successfully performed both in laboratory batch and continuous pilot-scale digestion tests. The addition of grease trap sludge to sewage sludge digesters was seen to increase the methane yield of 9-27% when 10-30% of sludge from grease traps (on VS-basis) was added. It was also seen that the grease trap sludge increases the methane yield without increasing the sludge production. Single-substrate digestion of grease trap sludge gave high methane potentials in batch tests, but could not reach stable methane production in continuous digestion.
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| 6. |
- Gruvberger, C, et al.
(författare)
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Sustainability concept for a newly built urban area in Malmo, Sweden
- 2003
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Ingår i: Water Science and Technology. - 0273-1223. ; 47:7-8, s. 33-39
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Tidskriftsartikel (refereegranskat)abstract
- National goals regarding sustainable development from the Swedish government were decisive in the planning process when the newly built city district Western Harbour was established in the city of Malmo, Sweden. A systems analysis was used as a tool for evaluating different collection and treatment systems. This type of analysis does not include more subjective factors such as acceptance of organic fertilisers based on human waste and user acceptance of collection schemes. These aspects, however, will often determine the success of a technical solution. When the system for collection and treatment of solid organic waste and wastewater was designed, both subjective and objective factors were considered. This meant that a centralised solution for wastewater treatment was selected. In order to facilitate a more sustainable solution for sludge management a treatment process with recovery of phosphorus will have to be introduced. Organic waste is sorted out and treated in an anaerobic digestion process. Source sorting of solid organic waste has been difficult to implement in Sweden due to inadequate sorting discipline. As a consequence two relatively new systems are tested in the area. A comprehensive evaluation will be carried out during a period of two years.
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| 7. |
- Gustavsson, DJI, et al.
(författare)
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Anammox i Huvudströmmen - Vad är problemet?
- 2012
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Ingår i: Vatten: tidskrift för vattenvård /Journal of Water Management and research. - 0042-2886. ; 68:3, s. 195-208
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Tidskriftsartikel (refereegranskat)abstract
- When Swedish wastewater treatment plants were rebuilt to include enhanced nitrogen removal, anaerobic ammonium oxidation (anammox) was far from being a mature technology. During the 2000s, the process has been introduced into sludge liquor treatment worldwide. The step to introduce anammox in the mainstream is decreasing, but lab- and pilot-scale studies on real municipal wastewater are yet lacking. However, several pilot studies are ongoing or will be started shortly. Difficulties to introduce anammox in the mainstream are still many. Anammox is favored by a separate treatment step for COD removal, by outcompeting nitrite oxidisers with the help of low oxygen concentrations, intermittent aeration and competition with anammox bacteria for nitrite, and by having a long solid retention time. Moving Bed Biofilm Reactor (MBBR), granular reactors, and activated sludge with hydrocyclones to recirculate anammox granules in the excess sludge, are the most promising systems. Sjölunda wastewater treatment plant in Malmö, Sweden, has a separate step for COD removal and denitrification is based on an MBBR for post-denitrification with methanol. VA SYD is now starting a pilot project to study the nitritation-anammox process in the mainstream in an MBBR with the aim of achieving a robust process, and to be able to use the already existing MBBRs for anammox in the mainstream.
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| 8. |
- Henze, M, et al.
(författare)
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Effect of solids retention time and wastewater characteristics on biological phosphorus removal
- 2002
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Ingår i: Water Science and Technology. - 0273-1223. ; 45:6, s. 137-144
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Tidskriftsartikel (refereegranskat)abstract
- The paper deals with the effect of wastewater, plant design and operation in relation to biological nitrogen and phosphorus removal and the possibilities to model the processes. Two Bio-P pilot plants were operated for 2.5 years in parallel receiving identical wastewater. The plants had SRT of 4 and 21 days, the latter had nitrification and denitrification. The plant with 4 days SRT had much more variable biomass characteristics, than the one with the high SRT. The internal storage compounds, PHA, were affected significantly by the concentration of fatty acids or other easily degradable organics in the wastewater, and less by the plant lay-out. The phosphorus removal is mainly dependent on availability in the wastewater of fatty acids but also by the suspended solids in the effluent, which is higher in the plant with nitrification-denitrification, probably due to a higher SVI or denitrification in the settler. The addition of glucose to the influent seems to have an effect on the performance of the plants similar to that of acetic acid. In spite of great load variations over time to the pilot plants and the different operational modes, the study of population dynamics showed less significant variations with time which has importance in relation to modelling. The overall conclusion of the comparison between the two plants is that the biological phosphorus removal efficiency under practical operating conditions is affected by the SRT in the plant and the wastewater composition. Thus great care should be taken when extrapolating results from one type of plant to another. Indirectly the experiments confirm that results from lab-experiments with artificial wastewater are difficult to extrapolate through modelling to real life wastewater and conditions. The 2.5 years time series can be valuable in verification of models for Nitrogen and Enhanced Biological Phosphorus Removal.
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| 9. |
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| 10. |
- la Cour Jansen, Jes, et al.
(författare)
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Digestion of sludge and organic waste in the sustainability concept for Malmo, Sweden
- 2004
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Ingår i: Water Science and Technology. - 0273-1223. ; 49:10, s. 163-169
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Tidskriftsartikel (refereegranskat)abstract
- Anaerobic digestion of sludge has been part of the treatment plant in Malmo for many years and several projects on optimisation of the digestion process have been undertaken in full scale as well as in pilot scale. In order to facilitate a more sustainable solution in the future for waste management,solid waste organic waste is sorted out from households for anaerobic treatment in a newly built city district. The system for treatment of the waste is integrated in a centralised solution located at the existing wastewater treatment plant. A new extension of the digester capacity enables separate as well as co-digestion of sludge together with urban organic waste from households, industry, restaurants, big kitchens, food stores, supermarkets, green markets etc. for biogas production and production of fertiliser. Collection and pre-treatment of different types of waste are in progress together with examination of biogas potential for different types of organic waste. Collection of household waste as well as anaerobic digestion in laboratory and pilot scale has been performed during the last year. It is demonstrated that organic household waste can be digested separately or in combination with sludge. In the latter case a higher biogas yield is found than should be expected from digestion of the two materials separately. Household waste from a system based on collection of organic waste from grinders could be digested at mesophilic conditions whereas digestion failed at thermophilic conditions.
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| 11. |
- Lee, N, et al.
(författare)
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Long-term population dynamics and in situ physiology in activated sludge systems with enhanced biological phosphorus removal operated with and without nitrogen removal
- 2003
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Ingår i: Systematic and Applied Microbiology. - : Elsevier BV. - 0723-2020. ; 26:2, s. 211-227
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Tidskriftsartikel (refereegranskat)abstract
- Quantitative fluorescence in situ hybridization (FISH) and the combination of FISH with microautoradiography (MAR) were used in order to study the long-term population dynamics (2.5 years) and the in situ physiology in two parallel activated sludge pilot systems with enhanced biological phosphorus removal (EBPR). The two systems received the same influent wastewater, but were differently operated (with and without nitrogen removal, respectively). Both systems showed a significant P removal that increased when different substrates (phosphorus (P), acetate and glucose, respectively) were added to the influent wastewater. Rhodocyclus-related bacteria were present in both systems in significant numbers (ranging from 4 to 28%) throughout the whole period. This supports the hypothesis that these bacteria occur in significant numbers in different types of well-operating EBPR activated sludge processes. However, we observed a lower correlation (<0.5) for the amount of Rhodocyclus-related bacteria to the P content in activated sludge than previous studies (>0.9). The Actinobacteria were the only additional group of bacteria which showed a similar degree of correlation to the P content in activated sludge as the Rhodocyclus-related bacteria - but only for the system without nitrogen removal. Significant amounts (less than or equal to12%) of glycogen-accumulating bacteria (GAOs) were detected in the system with nitrogen removal (but not in the other system), but had no, in contrast to previous observations, apparent negative effect on the overall EBPR performance. FISH-MAR indicated that a significant part of the Betaproteobacteria (part of them identified as Rhodocyclus-related bacteria) as well as the Actinobacteria were able to take up P-33(i), [H-3]-acetate and [H-3]-glucose under anaerobic-aerobic conditions. The contribution of anoxic P-33(i) uptake under alternating anaerobic-anoxic conditions was significantly lower. Interestingly, not all Rhodocyclus-related bacteria showed uptake of these three radioactive substrates. This may be due to differences in metabolic state, physiological potential or genotype, not detectable by the present probe set for Rhodocyclus-related bacteria. Comparison of the P-33(i), [H-3]-acetate and [H-3]-glucose uptake by activated sludge after different fixation and incubation procedures showed that a part of the observed P-33(i), uptake may have been caused by a combination of a biological and chemical or biologically induced chemical P adsorption.
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| 12. |
- Lee, N, et al.
(författare)
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Population dynamics in wastewater treatment plants with enhanced biological phosphorus removal operated with and without nitrogen removal
- 2002
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Ingår i: Water Science and Technology. - 0273-1223. ; 46:1-2, s. 163-170
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Tidskriftsartikel (refereegranskat)abstract
- The population dynamics of activated sludge in a pilot plant with two activated sludge systems, both designed for enhanced biological phosphorus removal (EBPR), but one of them with (BNP) and the other without (BP) nitrogen removal, was monitored during a period of 2.5 years. The influent water to the pilot plant was periodically manipulated by external addition of phosphorus (P), acetate and glucose, respectively. The population dynamics and the in situ physiology were monitored by quantitative fluorescence in situ hybridization (FISH) and microautoradiography. Significant P removal was observed in both systems throughout the whole period, with significant increases of the P removal when substrates were dosed. The activated sludge in both systems contained large amounts of dense clusters of gram-negative, methylene-blue staining coccoid rods during the whole period. A large part of the clusters belonged to the beta Proteobacteria, whereas the rest of the clusters belonged either to the Actinobacteria or to the a Proteobacteria. The relative abundance of Rhodocyclus-related bacteria in the activated sludge varied significantly in both systems during the whole period (from 6 to 18% in BNP, and from 4 to 28% in BP). However, no statistically significant correlation of the Rhodocyclus-related nor any of the other investigated bacterial groups to the P content of the activated sludge (correlation for all groups investigated was always <0.5) was observed. A significant P-33(i) uptake was observed by the β Proteobacteria (part of them Rhodocyclus-related, the identity of the rest unknown) and the Actinobacteria. However, not all of the Rhodocyclus-related bacteria showed P-33(i) uptake. The P removal in the investigated plants is thus believed to be mediated by a mixed population consisting of a part of the Rhodocyclus-related bacteria, the Actinobacteria and other, yet unidentified bacteria.
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| 13. |
- Olsson, Gustaf, et al.
(författare)
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Operation and Control of Wastewater Treatment - A Scandinavian Perspective over 20 years
- 1998
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Ingår i: Water Science and Technology. - 0273-1223. ; 37:12, s. 1-13
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Tidskriftsartikel (refereegranskat)abstract
- This paper aims to review the development of wastewater treatment operation and control over the last two decades. Due to the authors' background and experiences there is a special emphasis on the Scandinavian perspective. Since the developments in these countries have been in the forefront in the wastewater treatment area we believe that it may give a reasonable view of the progress also in other countries. The results of regulatory, technical and economical driving forces are discussed. A brief historical review of the progress of nutrient removal is made, and current methodologies for the operation and control of nutrient removal systems is reviewed.
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| 14. |
- Olsson, H., et al.
(författare)
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Increased plasma prolactin levels in a group of men with breast cancer - a preliminary study
- 1990
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Ingår i: Anticancer research. - 0250-7005. ; 10:1, s. 59-62
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Tidskriftsartikel (refereegranskat)abstract
- Gonadal and hypophyseal hormones were investigated in 15 males with breast cancer and 15 tumour referents, on average 1 month postoperatively. Plasma prolactin was found to be significantly more often elevated in men with breast cancer compared with referents (p <0.005). Another group of men with breast cancer disclosed a tendency for lower S-FSH levels compared with the referents (p <0.01). No significant difference was seen between cases and referents regarding S-LH, p-estradiol or p-testosterone. The size of the primary breast tumour was correlated with a higher prolactin level. The findings lend support to a theory implicating prolactin and possibly prolactinomas as a risk factor for the disease in males.
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| 15. |
- Ranstam, J., et al.
(författare)
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Survival in breast cancer and age at start of oral contraceptive usage
- 1991
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Ingår i: Anticancer research. - 0250-7005. ; 11:6, s. 2043-2046
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Tidskriftsartikel (refereegranskat)abstract
- In general, findings in studies on oral contraceptives (OCs) and breast cancer have not indicated prognosis to be worse among users of OCs. In few studies, however, has age at the start of OC usage been considered as a prognostic factor. In the present study prognosis in breast cancer is compared with OC usage particularly with age at the start of OC usage among 193 consecutive patients at the Department of Oncology University Hospital Lund. An earlier series of 193 breast cancer patients at Malmo General Hospital is included for comparisons. In the Lund series five-year survival was 62% among women who started to use OCs before the age of 20, 78% among those who started to use OCs between the ages of 20 and 25, and 86% among non-users and those who started to use OCs after the age of 25 (p = 0.009 test for homogeneity). Although age was found to be a prognostic factor in the Lund series (RR = 0.90, p = 0.001) this was not so in the earlier (older) Malmo series. The relationship with age differed significantly between the two series (p = 0.003) suggesting the apparent effect of age at diagnosis to be a cohort effect due to the introduction of OCs during the sixties. The age-specific relationship between survival and OC usage would seem to indicate the presence of a biological mechanism in which OCs may participate during precancerous and early stages of breast cancer.
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| 16. |
- Starokozhko, Viktoriia, et al.
(författare)
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Maintenance of drug metabolism and transport functions in human precision-cut liver slices during prolonged incubation for 5 days
- 2017
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Ingår i: Archives of Toxicology. - : Springer. - 0340-5761 .- 1432-0738. ; 91:5, s. 2079-2092
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Tidskriftsartikel (refereegranskat)abstract
- Human precision-cut liver slices (hPCLS) are a valuable ex vivo model that can be used in acute toxicity studies. However, a rapid decline in metabolic enzyme activity limits their use in studies that require a prolonged xenobiotic exposure. The aim of the study was to extend the viability and function of hPCLS to 5 days of incubation. hPCLS were incubated in two media developed for long-term culture of hepatocytes, RegeneMed(®), and Cellartis(®), and in the standard medium WME. Maintenance of phase I and II metabolism was studied both on gene expression as well as functional level using a mixture of CYP isoform-specific substrates. Albumin synthesis, morphological integrity, and glycogen storage was assessed, and gene expression was studied by transcriptomic analysis using microarrays with a focus on genes involved in drug metabolism, transport and toxicity. The data show that hPCLS retain their viability and functionality during 5 days of incubation in Cellartis(®) medium. Albumin synthesis as well as the activity and gene expression of phase I and II metabolic enzymes did not decline during 120-h incubation in Cellartis(®) medium, with CYP2C9 activity as the only exception. Glycogen storage and morphological integrity were maintained. Moreover, gene expression changes in hPCLS during incubation were limited and mostly related to cytoskeleton remodeling, fibrosis, and moderate oxidative stress. The expression of genes involved in drug transport, which is an important factor in determining the intracellular xenobiotic exposure, was also unchanged. Therefore, we conclude that hPCLS cultured in Cellartis(®) medium are a valuable human ex vivo model for toxicological and pharmacological studies that require prolonged xenobiotic exposure.
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| 17. |
- Tykesson, Eva, et al.
(författare)
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Use of phosphorus release batch tests for modelling an EBPR pilot plant
- 2002
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Ingår i: Water Science & Technology. - 0273-1223. ; 45:6, s. 99-106
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Konferensbidrag (refereegranskat)abstract
- The aim of this study was to evaluate how routinely performed phosphorus release tests could be used when modelling enhanced biological phosphorus removal (EBPR) using activated sludge models such as ASM2d. A pilot plant with an extensive analysis programme was used as basis for the simulations. Without any calibration the prediction of phosphorus removal was poor and the initial release rates from the simulations were not similar to those found from the laboratory tests. A period with low organic loading was chosen as a calibration period. In this period averages of daily influent measurements were used as influent parameters. First, calibration was performed in order to fit effluent COD and MLVSS in the sludge. Next, the phosphorus content in the sludge was decreased to the measured level by decreasing the fermentation rate. Finally, the initial phosphorus release rate was calculated from a simulated batch test and the PHA uptake rate was increased to fit this release rate with the average initial rates from laboratory batch tests performed during the period. The calibrated model was verified with data from the subsequent period where acetate was dosed.
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