| 1. |
|
|
| 2. |
|
|
| 3. |
- Cederberg, B., et al.
(författare)
-
Tvåvingar
- 2010
-
Ingår i: Rödlistade arter i Sverige 2010. - 9789188506351 ; , s. 185-202
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Abstract is not available
|
|
| 4. |
|
|
| 5. |
|
|
| 6. |
- Ostergaard, Henrik, et al.
(författare)
-
Prolonged half-life and preserved enzymatic properties of factor IX selectively PEGylated on native N-glycans in the activation peptide
- 2011
-
Ingår i: Blood. - : American Society of Hematology. - 1528-0020 .- 0006-4971. ; 118:8, s. 2333-2341
-
Tidskriftsartikel (refereegranskat)abstract
- Current management of hemophilia B entails multiple weekly infusions of factor IX (FIX) to prevent bleeding episodes. In an attempt to make a longer acting recombinant FIX (rFIX), we have explored a new releasable protraction concept using the native N-glycans in the activation peptide as sites for attachment of polyethylene glycol (PEG). Release of the activation peptide by physiologic activators converted glycoPEGylated rFIX (N9-GP) to native rFIXa and proceeded with normal kinetics for FXIa, while the Km for activation by FVIIa-tissue factor (TF) was increased by 2-fold. Consistent with minimal perturbation of rFIX by the attached PEG, N9-GP retained 73%-100% specific activity in plasma and whole-blood-based assays and showed efficacy comparable with rFIX in stopping acute bleeds in hemophilia B mice. In animal models N9-GP exhibited up to 2-fold increased in vivo recovery and a markedly prolonged half-life in mini-pig (76 hours) and hemophilia B dog (113 hours) compared with rFIX (16 hours). The extended circulation time of N9-GP was reflected in prolonged correction of coagulation parameters in hemophilia B dog and duration of effect in hemophilia B mice. Collectively, these results suggest that N9-GP has the potential to offer efficacious prophylactic and acute treatment of hemophilia B patients at a reduced dosing frequency. (Blood. 2011; 118(8): 2333-2341)
|
|
| 7. |
- von Euler, Mia, 1967-, et al.
(författare)
-
Inhalation of low concentrations of toluene induces persistent effects on a learning retention task, beam-walk performance, and cerebrocortical size in the rat
- 2000
-
Ingår i: Experimental Neurology. - New York, USA : Academic Press. - 0014-4886 .- 1090-2430. ; 163:1, s. 1-8
-
Tidskriftsartikel (refereegranskat)abstract
- The organic solvent toluene is widely used in industry. The threshold limit value for extended occupational exposure to toluene is presently set to 200 ppm in the United States. We have investigated the effect of an inhalation exposure of 80 ppm for 4 weeks (6 h/day, 5 days/week), followed by a postexposure period of at least 4 weeks, on behavior and brain features in the rat. Toluene exposure appeared to affect spatial memory, since toluene-exposed rats showed a longer time in the correct quadrant in a Morris swim maze. This effect may indicate that the exposed rats used their praxis strategy longer before they started to look for the platform elsewhere. Toluene-exposed rats showed trends for increases in both locomotion and rearing behaviors and a significantly reduced beam-walk performance. The area of the cerebral cortex, especially the parietal cortex, was decreased by 6-10% in toluene-exposed rats, as shown by magnetic resonance imaging of living rats and autoradiograms of frozen brain sections. The K(D) and B(max) values of the dopamine D(3) agonist [(3)H]PD 128907 were not affected by toluene, as measured in caudate-putamen and subcortical limbic area using biochemical receptor binding assays and in caudate-putamen and islands of Calleja using quantitative receptor autoradiography. Hence, previously demonstrated persistent effects by toluene on the binding characteristics of radioligands binding to both D(2) and D(3) receptors seem to indicate a persistent effect of toluene selectively on dopamine D(2) receptors. Taken together, the present results indicate that exposure to low concentrations of toluene leads to persistent effects on cognitive, neurological, and brain-structural properties in the rat.
|
|
| 8. |
|
|
| 9. |
|
|
| 10. |
|
|
| 11. |
- Andersson, Benita, 1951-, et al.
(författare)
-
Temporal Profile of Ultrastructural Changes in Cortical Neurons after a Compression Lesion
- 2006
-
Ingår i: Physiological Research. - : Institute of Physiology of the Czech Academy of Sciences. - 0862-8408 .- 1802-9973. ; 55:3, s. 339-348
-
Tidskriftsartikel (refereegranskat)abstract
- We studied the occurrence of apoptosis and secondary delayed cell death at various time points in the penumbra zone, which is the target for therapeutic intervention after stroke. A compression lesion was induced in the right sensory motor cortex of rat brains. At 0.5, 1, 3, 6, 12, 24, 48 and 72 h after lesioning, motor functions were evaluated by behavioral tests, and cortical layers IV and V were examined by electron microscopy. Behavioral recovery was observed at 48 h after lesioning. At 0.5-1 h in the lesioned area, the neuropil was expanded and contained affected cells. Apoptotic cells were found between 0.5-72 h, and at 12 h, 47.3 % of the total cell number was apoptotic cells. On the contralateral side, cells showed an enlarged endoplasmic reticulum at 3 h, indicating secondary delayed cell death. Our results show that a compression lesion is a useful model for studying ultrastructural changes in injured cells. The lesion results in the penumbra zone with apoptotic cell death between 0.5-72 h. As secondary delayed cell death occurred on the contralateral side at three hours after lesioning might be the time period during which injured, but still viable, neurons can be targets for acute treatment.
|
|
| 12. |
|
|
| 13. |
- Arfvidsson, J., et al.
(författare)
-
A method for status determination and risk assessment of energy measures in historic buildings
- 2021. - 1
-
Ingår i: IOP Conference Series : Earth and Environmental Science - Earth and Environmental Science. - : IOP Publishing. - 1755-1307 .- 1755-1315. ; 863
-
Konferensbidrag (refereegranskat)abstract
- Implementing energy efficiency measures in historic buildings is a challenging task and require knowledge in a number of different disciplines. The measures to reduce energy use must create a good indoor environment without jeopardizing the loss of important cultural historical values or entailing damages to building materials or load-bearing structures. Building antiquarian-, building biological- and building physical aspects are central, and must be taken into account in the entire process, from planning to implementation of energy update measures in historic buildings. This paper presents a methodology for status determination and risk assessment of energy measures in historic buildings. The method (KuReRA) has been developed in collaboration between curators, building biologists and building physicists.
|
|
| 14. |
|
|
| 15. |
|
|
| 16. |
|
|
| 17. |
|
|
| 18. |
|
|
| 19. |
|
|
| 20. |
|
|
| 21. |
|
|
| 22. |
|
|
| 23. |
|
|
| 24. |
|
|
| 25. |
|
|
| 26. |
|
|
| 27. |
- Hwang, F. H., et al.
(författare)
-
Diffusion and clearance of superparamagnetic iron oxide nanoparticles infused into the rat striatum studied by MRI and histochemical techniques
- 2011
-
Ingår i: Nanotechnology. - : IOP Publishing. - 0957-4484 .- 1361-6528. ; 22:1, s. 015103-
-
Tidskriftsartikel (refereegranskat)abstract
- The purpose of the present study was to investigate, by MRI and histochemical techniques, the diffusion and clearance abilities of superparamagnetic iron oxide nanoparticles (SPION) coated with dextran (Dextran-SPION) and gold (Au-SPION) following their local infusions into the rat brain. In separate groups of anesthetized rats, the Dextran-SPION and Au-SPION were infused at concentrations of 0.01, 0.1, 1 and 5 mu g Fe/0.5 mu l and at the flow rate of 0.5 mu l min(-1) into the left and right striata, respectively. Repetitive T2-weighted spin-echo MRI scans were performed at time intervals of 1, 6, 12, 24, 48, 72 h, and one, two and eight weeks after inoculation. Following infusion of Dextran-SPION (0.1 mu g and 1 mu g Fe), the maximal distribution volume was observed at about 12-24 h after inoculation and two weeks later the Fe signals were undetectable for the lower dose. On the other hand, Au-SPION remained tightly localized in the closest vicinity of the infusion site as revealed by unchanged MRI signal intensities and strong histochemical staining of Fe2+ and Fe3+ ions in the corresponding brain slices. Immunohistochemical staining of astrocytic and microglial reactions revealed that there were no marked differences in GFAP, VIM or OX-42 labeling observed between the nanoparticle types, however the astrocytic reaction was more pronounced in rats receiving nanoparticles compared to the control (aCSF-infused) rats. In conclusion, the present data demonstrate that the viral-sized Dextran-SPION were able to diffuse freely through the interstitial space of the brain being progressively cleared out from the infusion site within two weeks. Thus, Dextran-SPION could be beneficially used in MRI-guided diagnostic applications such as in experimental oncology or as labels and carriers for targeted drug delivery, whereas Au-SPION could be used for labeling and tracking the transplanted stem cells in experimental MRI.
|
|
| 28. |
- Janson, A M, et al.
(författare)
-
Protective effects of chronic nicotine treatment on lesioned nigrostriatal dopamine neurons in the male rat
- 1989
-
Ingår i: Progress in Brain Research. - 1875-7855. ; 79, s. 257-265
-
Tidskriftsartikel (refereegranskat)abstract
- The present results demonstrate that chronic nicotine treatment can in part protect against mechanically-induced and neurotoxin-induced degeneration of nigrostriatal DA neurons. These results indicate that in sufficient doses chronic treatment with nicotine may be considered in the pharmacological treatment of Parkinson's disease. It remains to be demonstrated whether these protective actions can be extended to include also other injured neurons such as the cholinergic neurons, known to be severely affected in Alzheimer's disease.
|
|
| 29. |
- Kim, D. K., et al.
(författare)
-
Characterization and MRI study of surfactant-coated superparamagnetic nanoparticles administered into the rat brain
- 2001
-
Ingår i: Journal of Magnetism and Magnetic Materials. - 0304-8853 .- 1873-4766. ; 225:02-jan, s. 256-261
-
Tidskriftsartikel (refereegranskat)abstract
- A pre-clinical study has been carried out for the utilization of magnetite (Fe-3 O-4) nanoparticles as a diagnostic tracer for MRI. Surfactant-coated Fe3O4 nanoparticles have been synthesized by a chemical coprecipitation method with a narrow particle size of around 6 nm. Preliminary experiments demonstrated the feasibility of using superparamagnetic Fe-3 O-4 nanoparticles as contrast agents in MR imaging.
|
|
| 30. |
- Kim, D. K., et al.
(författare)
-
Starch-coated superparamagnetic nanoparticles as MR contrast agents
- 2003
-
Ingår i: Chemistry of Materials. - : American Chemical Society (ACS). - 0897-4756 .- 1520-5002. ; 15:23, s. 4343-4351
-
Tidskriftsartikel (refereegranskat)abstract
- Superparamagnetic iron oxide nanoparticles (SPION) with suitable bio-compatible coatings have been used in biomedicine, particularly in magnetic resonance imaging (MRI), tissue engineering, and drug delivery applications. In this study, we describe the synthesis of SPION and its use for experimental in-vivo applications in MRI. SPION with a mean size of 6 nm have been prepared under inert atmosphere, in a polymeric starch matrix, by controlled chemical coprecipitation of magnetite phase from aqueous solutions containing suitable salts of Fe2+ and Fe3+. X-ray powder diffraction was used to confirm a pure magnetite phase for the SPION. The influence of oxidizing agents on the cleavage of the starch chains was investigated by changing the concentration of H2O2. An aqueous solution of H2O2/NaOH cleaves the glycosidic bonds and reduces the polymer chains to a critical average molecular weight. From the dynamic light scattering (DLS) size distribution, the bulk agglomeration size was decreased by approximately 50% of the bulk size when treated by H2O2. Freshly synthesized starch-coated SPION in buffered artificial cerebro-spinal fluid were injected into the brain parenchyma of anaesthetized rats for in-vivo monitoring. Analysis of T-2*-weighted images and T-2*-maps revealed formation of a. concentration gradient for the SPION at the injection site, indicating SPION dispersion in the living brain parenchyma from the center of the injection site toward the periphery. The starch-coated SPION show a biocompatibility and possibility of being transported in the extracellular space as well as being internalized in nerve cells.
|
|
| 31. |
|
|
| 32. |
|
|
| 33. |
|
|
| 34. |
|
|
| 35. |
|
|
| 36. |
|
|
| 37. |
|
|
| 38. |
|
|
| 39. |
|
|
| 40. |
|
|
| 41. |
|
|
| 42. |
|
|
