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- Bontidean, Ibolya, et al.
(författare)
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Novel synthetic phytochelatin-based capacitive biosensor for heavy metal ion detection
- 2003
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Ingår i: Biosensors & Bioelectronics. - 1873-4235. ; 18:5-6, s. 547-553
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Tidskriftsartikel (refereegranskat)abstract
- A novel capacitance biosensor based on synthetic phytochelatins for sensitive detection of heavy metals is described. Synthetic phytochelatin (Glu-Cys)20Gly (EC20) fused to the maltose binding domain protein was expressed in Escherichia coli and purified for construction of the biosensor. The new biosensor was able to detect Hg2+, Cd2+, Pb2+, Cu2+ and Zn2+ ions in concentration range of 100 fM–10 mM, and the order of sensitivity was SZn>SCu>SHg>>SCdSPb. The biological sensing element of the sensor could be regenerated using EDTA and the storage stability of the biosensor was 15 days.
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