| 1. |
- Kehoe, Laura, et al.
(författare)
-
Make EU trade with Brazil sustainable
- 2019
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 364:6438, s. 341-
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 2. |
- Baumann, Anne, et al.
(författare)
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HAMLET Forms Annular Oligomers When Deposited with Phospholipid Monolayers
- 2012
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Ingår i: Journal of Molecular Biology. - : Elsevier BV. - 1089-8638 .- 0022-2836. ; 418:1-2, s. 90-102
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Tidskriftsartikel (refereegranskat)abstract
- Recently, the anticancer activity of human a-lactalbumin made lethal to tumor cells (HAMLET) has been linked to its increased membrane affinity in vitro, at neutral pH, and ability to cause leakage relative to the inactive native bovine alpha-lactalbumin (BLA) protein. In this study, atomic force microscopy resolved membrane distortions and annular oligomers (AOs) produced by HAMLET when deposited at neutral pH on mica together with a negatively charged lipid monolayer. BLA, BAMLET (HAMLET's bovine counterpart) and membrane-binding Peptide C, corresponding to BLA residues 75-100, also form AO-like structures under these conditions but at higher subphase concentrations than HAMLET. The N-terminal Peptide A, which binds to membranes at acidic but not at neutral pH, did not form AOs. This suggests a correlation between the capacity of the proteins/peptides to integrate into the membrane at neutral pH as observed by liposome content leakage and circular dichroism experiments and the formation of AOs, albeit at higher concentrations. Formation of AOs, which might be important to HAMLET's tumor toxic action, appears related to the increased tendency of the protein to populate intermediately folded states compared to the native protein, the formation of which is promoted by, but not uniquely dependent on, the oleic acid molecules associated with HAMLET. (C) 2012 Elsevier Ltd. All rights reserved.
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| 3. |
- Baumann, Anne, et al.
(författare)
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Tyrosine Hydroxylase Binding to Phospholipid Membranes Prompts Its Amyloid Aggregation and Compromises Bilayer Integrity
- 2016
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 6
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Tidskriftsartikel (refereegranskat)abstract
- Tyrosine hydroxylase (TH), a rate-limiting enzyme in the synthesis of catecholamine neurotransmitters and hormones, binds to negatively charged phospholipid membranes. Binding to both large and giant unilamellar vesicles causes membrane permeabilization, as observed by efflux and influx of fluorescence dyes. Whereas the initial protein-membrane interaction involves the N-terminal tail that constitutes an extension of the regulatory ACT-domain, prolonged membrane binding induces misfolding and self-oligomerization of TH over time as shown by circular dichroism and Thioflavin T fluorescence. The gradual amyloid-like aggregation likely occurs through cross-beta interactions involving aggregation-prone motives in the catalytic domains, consistent with the formation of chain and ring-like protofilaments observed by atomic force microscopy in monolayer-bound TH. PC12 cells treated with the neurotoxin 6-hydroxydopamine displayed increased TH levels in the mitochondrial fraction, while incubation of isolated mitochondria with TH led to a decrease in the mitochondrial membrane potential. Furthermore, cell-substrate impedance and viability assays showed that supplementing the culture media with TH compromises cell viability over time. Our results revealed that the disruptive effect of TH on cell membranes may be a cytotoxic and pathogenic factor if the regulation and intracellular stability of TH is compromised.
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| 4. |
- Baumann, Henrikke, 1964, et al.
(författare)
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Det specifika med miljösystemanalysen
- 1999
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Sammanfattning av diskussion om vad forskning i ämnet miljösystemanalys innebär och innefattar.
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| 5. |
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| 6. |
- Baumann, Henrikke, 1964, et al.
(författare)
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LCA i ett nötskal
- 2014
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Rapport (övrigt vetenskapligt/konstnärligt)
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| 7. |
- Baumann, Henrikke, 1964, et al.
(författare)
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Switching the focus from product function to business profit: Introducing Business Model LCA (BM-LCA)
- 2022
-
Ingår i: Proceedings of LCM 2021. - : EDP Sciences. ; 349
-
Konferensbidrag (refereegranskat)abstract
- Recent years have seen much interest in business models as vehicles towards sustainability, cf. [1]. Conventional LCA, however, fails to properly capture the environmental impacts of a business model. Here, we introduce the background and the principles of Business Model LCA, a new LCA methodology for the assessment of environmental performance of business models. Methodological innovations are based on an understanding of the difference and relatedness between product and business. The key innovation is that BM-LCA centres its analysis on the ’business model’ instead of the ’product function’ as in conventional LCA. This requires the functional unit to represent the business (e.g., as ‘profit per time unit’) and the need to couple the monetary flows of the business to the material and energy flows of the product system via a set of ’coupling’ equations. BM-LCA contributes to environmental business analysis and could open up a new avenue of research where LCA and business analysts collaborate on business model innovation for sustainability.
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| 8. |
- Baumann, Henrikke, 1964, et al.
(författare)
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The Hitch Hiker's Guide to LCA. An orientation in life cycle assessment methodology and application
- 2004
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- The environmental life cycle of a product consists of all the stages from raw material extraction through production and use to waste management. Life cycle assessment (LCA), then, is the assessment of the environmental impact of a product throughout its life cycle. The holistic perspective that LCA provides on the environmental performance of products has made it a central concept for both environmental management in industry and environmental policy-making in public government. This is a textbook on LCA for those who want to learn the practice of LCA, e.g. environmental engineers, environmental managers and ecodesigners. The title paraphrases Douglas AdamsÕ famous story The Hitch HikerÕs Guide to the Galaxy, in which the machine Deep Thought after seven and a half million years of computing come up with Ô42Õ as the answer to the Ôgreat Question of Life, the Universe and EverythingÕ. Expectations on LCA are often similarÑsimple answer to difficult environmental dilemmas, and the result often as incomprehensible as Ô42Õ, unless one knows how to interpret LCA methodology and results.LCA applications are manifold. LCA can be used in e.g. product development, marketing, production and waste management. There are special requirements on LCA methodology for each type of application and the book presents a broad repertoire of methodological alternatives in LCA, their different implications for LCA results and their usefulness in different applications.The book is organised in three parts covering LCA methodology, LCA applications and exercises on LCA. Two introductory chapters give a general overview of the LCA concept and its historical development. After that, LCA methodology is described in detail in six chapters. Different fields of LCA application are covered in five subsequent chapters. Since the aim of the book is to teach the execution of LCA, there are also a number of exercises. Smaller exercises train different aspects of LCA methodology and prepare for the larger ones, ten complete LCA exercise projects.
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| 9. |
- Baumann, Henrikke, 1964, et al.
(författare)
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The Usefulness of an Actor’s Perspective in LCA
- 2011
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Ingår i: Towards Life Cycle Sustainability Management. - Dordrecht : Springer Netherlands. - 9789400718982 ; , s. 73-83
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Konferensbidrag (refereegranskat)abstract
- This paper is an argumentation for adding an actor’s perspective to lifecycleassessment (LCA). The need for this perspective stems from a criticismabout the usefulness of LCA interpretation methods comparing the relativecontribution of life-cycle phases of a product. Our argumentation is based on fourpreviously published studies providing practical examples of how value chainactors’ influence may be considered in an LCA and the benefit of doing so.Manufacturing sector examples show how one company's influence can beillustrated in results and how it may relate all relevant emissions to its ownprocesses. The food sector study shows how to assess several value chain actors’individual improvement potential. The final example, taken from building sector,explore how to consider the fact that actors in one part of the value chain caninfluence other actors to improve.
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| 10. |
- Breznau, Nate, et al.
(författare)
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Observing many researchers using the same data and hypothesis reveals a hidden universe of uncertainty
- 2022
-
Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 119:44
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Tidskriftsartikel (refereegranskat)abstract
- This study explores how researchers analytical choices affect the reliability of scientific findings. Most discussions of reliability problems in science focus on systematic biases. We broaden the lens to emphasize the idiosyncrasy of conscious and unconscious decisions that researchers make during data analysis. We coordinated 161 researchers in 73 research teams and observed their research decisions as they used the same data to independently test the same prominent social science hypothesis: that greater immigration reduces support for social policies among the public. In this typical case of social science research, research teams reported both widely diverging numerical findings and substantive conclusions despite identical start conditions. Researchers expertise, prior beliefs, and expectations barely predict the wide variation in research outcomes. More than 95% of the total variance in numerical results remains unexplained even after qualitative coding of all identifiable decisions in each teams workflow. This reveals a universe of uncertainty that remains hidden when considering a single study in isolation. The idiosyncratic nature of how researchers results and conclusions varied is a previously underappreciated explanation for why many scientific hypotheses remain contested. These results call for greater epistemic humility and clarity in reporting scientific findings.
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| 11. |
- Böckin, Daniel, 1989, et al.
(författare)
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Business model life cycle assessment: A method for analysing the environmental performance of business
- 2022
-
Ingår i: Sustainable Production and Consumption. - : Elsevier. - 2352-5509. ; 32, s. 112-124
-
Tidskriftsartikel (refereegranskat)abstract
- This paper introduces business model life cycle assessment (BM-LCA), a new method for quantifying the environmental impacts of business models. Such a method is needed to guide business decisions towards decoupling economic activity from environmental impact. BM-LCA takes the business model itself as the unit of analysis and its economic performance as the basis of comparison. It can be applied to any type of business model involving material or resource use. In BM-LCA, monetary flows are coupled to material and energy flows. The methodology expands on conventional life cycle assessment (LCA) by elaborating the goal and scope definition and dividing it into two phases. The first descriptive phase details the business models to be compared. It includes a mapping of product chain actors and identifying business operations and transactions related to the product. The second coupling phase defines a profit-based functional unit and sets up the coupling equations expressing the economic relations to the product. Thereafter, conventional LCA procedures are followed to assess environmental impacts. The key innovation on LCA methodology is the development of a functional unit that captures the economic performance of a business model and links it to a product system. BM-LCA provides thus an important link between LCA and business competitive advantage.
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| 12. |
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| 13. |
- Böckin, Daniel, 1989, et al.
(författare)
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Environmental assessment of two business models - a life cycle comparison between a sales and a rental business model in the apparel sector in Sweden
- 2020
-
Rapport (övrigt vetenskapligt/konstnärligt)abstract
- This report presents and applies a new method for environmentally assessing business models from a life cycle perspective. The method is meant to be useful for companies to guide their business decisions toward decoupling of economic activity from environmental impact. The basis of the method is an integrated economic and environmental perspective and a functional unit based on profit is employed, after which Life Cycle Assessment (LCA) methodology is carried out. This Business Model LCA is applied on a Swedish company and their current business model (sales) compared to an alternative business model (rental). Jackets are chosen as the products to represent the business of the company. Results indicate that a company can achieve more decoupling by renting jackets instead of selling them, although the results are sensitive to some economic parameters (like rental efficiency and rental price) and to customer habits, which can significantly compromise the overall performance of the rental model. The central feature of the developed method is that, by coupling the economic and physical flows of a business model, it allows a company to directly see the impact of business decisions on their environmental performance and plan their activities accordingly.
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| 14. |
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| 15. |
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| 16. |
- Emmerich, Anne C., et al.
(författare)
-
Proteomics-Based Characterization of miR-574-5p Decoy to CUGBP1 Suggests Specificity for mPGES-1 Regulation in Human Lung Cancer Cells
- 2020
-
Ingår i: Frontiers in Pharmacology. - : Frontiers Media SA. - 1663-9812. ; 11
-
Tidskriftsartikel (refereegranskat)abstract
- MicroRNAs (miRs) are one of the most important post-transcriptional repressors of gene expression. However, miR-574-5p has recently been shown to positively regulate the expression of microsomal prostaglandin E-synthase-1 (mPGES-1), a key enzyme in the prostaglandin E2 (PGE2) biosynthesis, by acting as decoy to the RNA-binding protein CUG-RNA binding protein 1 (CUGBP1) in human lung cancer. miR-574-5p exhibits oncogenic properties and promotes lung tumor growth in vivo via induction of mPGES-1-derived PGE2 synthesis. In a mass spectrometry-based proteomics study, we now attempted to characterize this decoy mechanism in A549 lung cancer cells at a cellular level. Besides the identification of novel CUGBP1 targets, we identified that the interaction between miR-574-5p and CUGBP1 specifically regulates mPGES-1 expression. This is supported by the fact that CUGBP1 and miR-574-5p are located in the nucleus, where CUGBP1 regulates alternative splicing. Further, in a bioinformatical approach we showed that the decoy-dependent mPGES-1 splicing pattern is unique. The specificity of miR-574-5p/CUGBP1 regulation on mPGES-1 expression supports the therapeutic strategy of pharmacological inhibition of PGE2 formation, which may provide significant therapeutic value for NSCLC patients with high miR-574-5p levels.
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| 17. |
- Goffetti, Giulia, et al.
(författare)
-
Towards sustainable business models with a novel life cycle assessment method
- 2022
-
Ingår i: Business Strategy and the Environment. - : John Wiley & Sons. - 0964-4733 .- 1099-0836. ; 31:5, s. 2019-2035
-
Tidskriftsartikel (refereegranskat)abstract
- Business model (BM) innovation for sustainability is hampered by a lack of tools for environmental assessment and guidance at the BM level. Conventional life cycle assessment (LCA) neglects the economic and socio-technical mechanisms within a BM, and tools based on the BM canvas (BMC) cannot provide recommendations substantiated by environmental data. Here, a new method, BM-LCA, is applied to a case comparing the selling and renting of jackets, using profit as basis of comparison. Results identify how business parameters influence environmental performance, permitting analysis for decoupling within a business practice. This is made possible by the unique way the method links physical life cycle and the monetary flows of a BM. Usefulness of BM-LCA is discussed relative to BM innovation, business strategy and similar tools. BM-LCA provides insights into a broad range of BM elements and emerges as useful for business strategy. By measuring BM environmental performance, it helps determine what BM to compete with and support critical analysis of business against greenwashing. BM-LCA also enables identification of BM elements in greatest need of environmental innovation. BM-LCA appears as a promising tool for guiding business companies towards sustainability, filling a space between LCA and BMC. The method offers a practical way for business and LCA experts to merge their respective knowledge.
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| 18. |
- Holbech, Henrik, et al.
(författare)
-
Comparison of zebrafish (Danio rerio) and fathead minnow (Pimephales promelas) as test species in the Fish Sexual Development Test (FSDT).
- 2012
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Ingår i: Comparative Biochemistry and Physiology - Part C. - : Elsevier BV. - 1532-0456 .- 1878-1659. ; 155:2, s. 407-15
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Tidskriftsartikel (refereegranskat)abstract
- Results are presented from a validation (with 5 laboratories) of the Fish Sexual Development Test (FSDT) developed to detect endocrine disrupters (EDs) and included in the OECD (Organisation for Economic Co-operation and Development) working program. The aromatase-inhibiting fungicide prochloraz was tested in zebrafish (Danio rerio) and fathead minnow (Pimephales promelas). The fish were exposed during sexual differentiation and development from 0 to 60 days post hatch (dph). After exposure, the vitellogenin (VTG) concentrations were quantified in head/tail homogenate and the sex ratio was determined (defined as female, male, intersex or undifferentiated). NOEC/LOEC and EC(x) designs were compared to optimize the test approach. Results show that both species are highly sensitive to prochloraz during sexual development. They respond by skewing of the sex ratio towards male phenotype and by a VTG decline in females. The NOEC/LOEC approach is preferred because sex ratio is difficult to analyze with a regression model. The mean NOEC/LOEC for prochloraz on the sex ratio was 43.3/134 μg/L and 101/293 μg/L for zebrafish and fathead minnow, respectively. The mean NOEC/LOEC on the decline in female VTG concentration was 65/110 μg/L and ~30/68 μg/L respectively. In conclusion, zebrafish and fathead minnow are suitable species in the FSDT and their sexual differentiation is equally labile to EDs.
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| 19. |
- Lévy, Romain, et al.
(författare)
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Human CARMIL2 deficiency underlies a broader immunological and clinical phenotype than CD28 deficiency.
- 2023
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Ingår i: The Journal of experimental medicine. - : Rockefeller University Press. - 1540-9538 .- 0022-1007. ; 220:2
-
Tidskriftsartikel (refereegranskat)abstract
- Patients with inherited CARMIL2 or CD28 deficiency have defective T cell CD28 signaling, but their immunological and clinical phenotypes remain largely unknown. We show that only one of three CARMIL2 isoforms is produced and functional across leukocyte subsets. Tested mutant CARMIL2 alleles from 89 patients and 52 families impair canonical NF-κB but not AP-1 and NFAT activation in T cells stimulated via CD28. Like CD28-deficient patients, CARMIL2-deficient patients display recalcitrant warts and low blood counts of CD4+ and CD8+ memory T cells and CD4+ TREGs. Unlike CD28-deficient patients, they have low counts of NK cells and memory B cells, and their antibody responses are weak. CARMIL2 deficiency is fully penetrant by the age of 10 yr and is characterized by numerous infections, EBV+ smooth muscle tumors, and mucocutaneous inflammation, including inflammatory bowel disease. Patients with somatic reversions of a mutant allele in CD4+ T cells have milder phenotypes. Our study suggests that CARMIL2 governs immunological pathways beyond CD28.
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| 20. |
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| 21. |
- Mossberg, Ann-Kristin, et al.
(författare)
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HAMLET Interacts with Lipid Membranes and Perturbs Their Structure and Integrity.
- 2010
-
Ingår i: PloS one. - : Public Library of Science (PLoS). - 1932-6203. ; 5:2
-
Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: Cell membrane interactions rely on lipid bilayer constituents and molecules inserted within the membrane, including specific receptors. HAMLET (human alpha-lactalbumin made lethal to tumor cells) is a tumoricidal complex of partially unfolded alpha-lactalbumin (HLA) and oleic acid that is internalized by tumor cells, suggesting that interactions with the phospholipid bilayer and/or specific receptors may be essential for the tumoricidal effect. This study examined whether HAMLET interacts with artificial membranes and alters membrane structure. METHODOLOGY/PRINCIPAL FINDINGS: We show by surface plasmon resonance that HAMLET binds with high affinity to surface adherent, unilamellar vesicles of lipids with varying acyl chain composition and net charge. Fluorescence imaging revealed that HAMLET accumulates in membranes of vesicles and perturbs their structure, resulting in increased membrane fluidity. Furthermore, HAMLET disrupted membrane integrity at neutral pH and physiological conditions, as shown by fluorophore leakage experiments. These effects did not occur with either native HLA or a constitutively unfolded Cys-Ala HLA mutant (rHLA(all-Ala)). HAMLET also bound to plasma membrane vesicles formed from intact tumor cells, with accumulation in certain membrane areas, but the complex was not internalized by these vesicles or by the synthetic membrane vesicles. CONCLUSIONS/SIGNIFICANCE: The results illustrate the difference in membrane affinity between the fatty acid bound and fatty acid free forms of partially unfolded HLA and suggest that HAMLET engages membranes by a mechanism requiring both the protein and the fatty acid. Furthermore, HAMLET binding alters the morphology of the membrane and compromises its integrity, suggesting that membrane perturbation could be an initial step in inducing cell death.
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| 22. |
- Peters, Gregory, 1970, et al.
(författare)
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A Swedish comment on ‘review: the availability of life-cycle studies in Sweden’
- 2019
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Ingår i: International Journal of Life Cycle Assessment. - : Springer Science and Business Media LLC. - 1614-7502 .- 0948-3349. ; 24:10, s. 1758-1759
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The article entitled ‘Review: the availability of life-cycle studies in Sweden’ by Croft and colleagues (January 2019, volume 24, issue 1, pages 6–11) has puzzled many researchers in Sweden. The stated purpose of the article is to review the availability of water and carbon footprinting studies and life-cycle assessment (LCA) studies in Sweden. Despite its title and purpose suggesting otherwise, the article appears to be about the accessibility of life-cycle case studies from Sweden in South Africa. It is problematic that the article claims to be a review in the title and text, but is presented by the journal as a commentary. We believe that the article’s method is unclear and that its title and results are misleading. The authors of the article found only 12 academic papers, 10 academic theses, 8 company reports, and 1 presentation. This result significantly underestimates the actual production and availability of Swedish LCA case studies.
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| 23. |
- Pröbstel, Anne-Katrin, et al.
(författare)
-
Gut microbiota-specific IgA+ B cells traffic to the CNS in active multiple sclerosis
- 2020
-
Ingår i: Science immunology. - : American Association for the Advancement of Science. - 2470-9468. ; 5:53
-
Tidskriftsartikel (refereegranskat)abstract
- Changes in gut microbiota composition and a diverse role of B cells have recently been implicated in multiple sclerosis (MS), a central nervous system (CNS) autoimmune disease. Immunoglobulin A (IgA) is a key regulator at the mucosal interface. However, whether gut microbiota shape IgA responses and what role IgA+ cells have in neuroinflammation are unknown. Here, we identify IgA-bound taxa in MS and show that IgA-producing cells specific for MS-associated taxa traffic to the inflamed CNS, resulting in a strong, compartmentalized IgA enrichment in active MS and other neuroinflammatory diseases. Unlike previously characterized polyreactive anti-commensal IgA responses, CNS IgA cross-reacts with surface structures on specific bacterial strains but not with brain tissue. These findings establish gut microbiota-specific IgA+ cells as a systemic mediator in MS and suggest a critical role of mucosal B cells during active neuroinflammation with broad implications for IgA as an informative biomarker and IgA-producing cells as an immune subset to harness for therapeutic interventions.
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| 24. |
- Saul, Meike J., et al.
(författare)
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Mass Spectrometry-Based Proteomics Approach Characterizes the Dual Functionality of miR-328 in Monocytes
- 2019
-
Ingår i: Frontiers in Pharmacology. - : FRONTIERS MEDIA SA. - 1663-9812. ; 10
-
Tidskriftsartikel (refereegranskat)abstract
- MicroRNAs (miRs) are small noncoding RNAs which control the expression of target genes by either translational repression or RNA degradation, known as canonical miR functions. The recent discovery that miR-328 has a noncanonical function and can activate gene expression by antagonizing the activity of heterogeneous ribonuclear protein E2 (hnRNP E2) opens an unexplored and exciting field of gene expression regulation. The global importance of such noncanonical miR function is not yet known. In order to achieve a better understanding of the new miR activity, we performed a compartment specific tandem mass tag (TMT)-based proteomic analysis in differentiated MonoMac6 (MM6) cells, to monitor gene expression variations in response to miR-328 knockdown. We identified a broad spectrum of novel potential miR-328/hnRNP E2 and miR-328 targets involved in regulation of compartment specific cellular processes, such as inflammation or RNA splicing. This study provides first insights of the global significance of noncanonical miR function.
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| 25. |
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| 26. |
- Tikka, Saara, et al.
(författare)
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Congruence between NOTCH3 mutations and GOM in 131 CADASIL patients
- 2009
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Ingår i: Brain. - : Oxford University Press (OUP). - 0006-8950 .- 1460-2156. ; 132, s. 933-939
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Tidskriftsartikel (refereegranskat)abstract
- Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is the most common hereditary subcortical vascular dementia. It is caused by mutations in NOTCH3 gene, which encodes a large transmembrane receptor Notch3. The key pathological finding is the accumulation of granular osmiophilic material (GOM), which contains extracellular domains of Notch3, on degenerating vascular smooth muscle cells (VSMCs). GOM has been considered specifically diagnostic for CADASIL, but the reports on the sensitivity of detecting GOM in patients skin biopsy have been contradictory. To solve this contradiction, we performed a retrospective investigation of 131 Finnish, Swedish and French CADASIL patients, who had been adequately examined for both NOTCH3 mutation and presence of GOM. The patients were examined according to the diagnostic practice in each country. NOTCH3 mutations were assessed by restriction enzyme analysis of specific mutations or by sequence analysis. Presence of GOM was examined by electron microscopy (EM) in skin biopsies. Biopsies of 26 mutation-negative relatives from CADASIL families served as the controls. GOM was detected in all 131 mutation positive patients. Altogether our patients had 34 different pathogenic mutations which included three novel point mutations (p.Cys67Ser, p.Cys251Tyr and p.Tyr1069Cys) and a novel duplication (p.Glu434_Leu436dup). The detection of GOM by EM in skin biopsies was a highly reliable diagnostic method: in this cohort the congruence between NOTCH3 mutations and presence of GOM was 100. However, due to the retrospective nature of this study, exact figure for sensitivity cannot be determined, but it would require a prospective study to exclude possible selection bias. The identification of a pathogenic NOTCH3 mutation is an indisputable evidence for CADASIL, but demonstration of GOM provides a cost-effective guide for estimating how far one should proceed with the extensive search for a new or an uncommon mutations among the presently known over 170 different NOTCH3 gene defects. The diagnostic skin biopsy should include the border zone between deep dermis and upper subcutis, where small arterial vessels of correct size are located. Detection of GOM requires technically adequate biopsies and distinction of true GOM from fallacious deposits. If GOM is not found in the first vessel or biopsy, other vessels or additional biopsies should be examined.
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| 27. |
- Tillman, Anne-Marie, 1958, et al.
(författare)
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After 10 years and 300 students. Our LCA teaching experience.
- 2002
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Ingår i: Engineering Education in Sustainable Development. ; , s. 611-616
-
Konferensbidrag (refereegranskat)abstract
- Our LCA teaching experience started with organizing the first LCA course in Sweden in 1992 — it was a course on a national level for doctoral students. After that, we continued with one LCA course each year for undergraduate students (3rd or 4th year students). During the first years (1993-1995), the course was given as a summer course for international students. But since 1996, the LCA course is given as an ordinary, elective course for all engineering students at Chalmers, attracting som 30 students every year. Most of them are Chalmers students, but a number of students from Göteborg University, doctoral students and working engineers from around Sweden also find their way to our course. In addition to the LCA course, we also give "the 2 hour lecture" orientation about LCA in the compulsory environmental course for 1st/2nd year students at Chalmers, and we have run around 25 LCA projects as MSc Diploma projects. Based on our broad experience, we will talk about how LCA education can aim at different levels of understanding (from the 'know about" level, via the "reflect critically" level, to the "carry out" level) and what this implies concerning reading material, exercises, the use of LCA software and LCA manuals. We will also talk about student reactions to working in interdisciplinary groups and with open problems.
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| 28. |
- Tillman, Anne-Marie, 1958, et al.
(författare)
-
Choice of system boundaries in life cycle assessment
- 1994
-
Ingår i: Journal of Cleaner Production. - : Elsevier BV. - 0959-6526. ; 2:1, s. 21-29
-
Tidskriftsartikel (refereegranskat)abstract
- System boundaries in life cycle assessments (LCA) must be specified in several dimensions: boundaries between the technological system and nature, delimitations of the geographical area and time horizon considered, boundaries between production and production of capital goods and boundaries between the life cycle of the product studied and related life cycles of other products. Principles for choice of system boundaries are discussed, especially concerning the last dimension. Three methods for defining the contents of the analysed system in this respect are described: process tree, technological whole system and socio-economic whole system. The methods are described in the application's multi-output processes and cascade recycling, and examples are discussed. It is concluded that system boundaries must be relevant in relation to the purpose of an LCA, that processes outside the process tree in many cases have more influence on the result than details within the process tree, and that the different methods need to be further compared in practice and evaluated with respect to both relevance, feasibility and uncertainty.
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| 29. |
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| 30. |
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| 31. |
- Trompier, Francois, et al.
(författare)
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Investigation of the influence of calibration practices on cytogenetic laboratory performance for dose estimation
- 2017
-
Ingår i: International Journal of Radiation Biology. - : Informa UK Limited. - 0955-3002 .- 1362-3095. ; 93:1, s. 118-126
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: In the frame of the QA program of RENEB, an inter-laboratory comparison (ILC) of calibration sources used in biological dosimetry was achieved to investigate the influence of calibration practices and protocols on the results of the dose estimation performance as a first step to harmonization and standardization of dosimetry and irradiation practices in the European biological dosimetry network. Materials and methods: Delivered doses by irradiation facilities used by RENEB partners were determined with EPR/alanine dosimetry system. Dosimeters were irradiated in the same conditions as blood samples. A short survey was also performed to collect the information needed for the data analysis and evaluate the diversity of practices. Results: For most of partners the deviation of delivered dose from the targeted dose remains below 10%. Deviations larger than 10% were observed for five facilities out of 21. Origins of the largest discrepancies were identified. Correction actions were evaluated as satisfactory. The re-evaluation of some ILC results for the fluorescence in situ hybridization (FISH) and premature chromosome condensation (PCC) assays has been performed leading to an improvement of the overall performances. Conclusions: This work has shown the importance of dosimetry in radiobiology studies and the needs of harmonization, standardization in irradiation and dosimetry practices and educational training for biologists using ionizing radiation.
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