| 1. |
- Behra, Phani Rama Krishna, et al.
(författare)
-
Comparative genome analysis of Mycobacteria focusing on tRNA and non-coding RNA
- 2022
-
Ingår i: BMC Genomics. - : BioMed Central (BMC). - 1471-2164. ; 23
-
Tidskriftsartikel (refereegranskat)abstract
- Background: The Mycobacterium genus encompasses at least 192 named species, many of which cause severe diseases such as tuberculosis. Non-tuberculosis mycobacteria (NTM) can also infect humans and animals. Some are of emerging concern because they show high resistance to commonly used antibiotics while others are used and evaluated in bioremediation or included in anticancer vaccines.Results: We provide the genome sequences for 114 mycobacterial type strains and together with 130 available mycobacterial genomes we generated a phylogenetic tree based on 387 core genes and supported by average nucleotide identity (ANI) data. The 244 genome sequences cover most of the species constituting the Mycobacterium genus. The genome sizes ranged from 3.2 to 8.1 Mb with an average of 5.7 Mb, and we identified 14 new plasmids. Moreover, mycobacterial genomes consisted of phage-like sequences ranging between 0 and 4.64% dependent on mycobacteria while the number of IS elements varied between 1 and 290. Our data also revealed that, depending on the mycobacteria, the number of tRNA and non-coding (nc) RNA genes differ and that their positions on the chromosome varied. We identified a conserved core set of 12 ncRNAs, 43 tRNAs and 18 aminoacyl-tRNA synthetases among mycobacteria.Conclusions; Phages, IS elements, tRNA and ncRNAs appear to have contributed to the evolution of the Mycobacterium genus where several tRNA and ncRNA genes have been horizontally transferred. On the basis of our phylogenetic analysis, we identified several isolates of unnamed species as new mycobacterial species or strains of known mycobacteria. The predicted number of coding sequences correlates with genome size while the number of tRNA, rRNA and ncRNA genes does not. Together these findings expand our insight into the evolution of the Mycobacterium genus and as such they establish a platform to understand mycobacterial pathogenicity, their evolution, antibiotic resistance/tolerance as well as the function and evolution of ncRNA among mycobacteria.
|
|
| 2. |
- Behra, Phani Rama Krishna, et al.
(författare)
-
Comparative genomics of Mycobacterium mucogenicum and Mycobacterium neoaurum clade members emphasizing tRNA and non-coding RNA
- 2019
-
Ingår i: BMC Evolutionary Biology. - : BMC. - 1471-2148. ; 19
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Mycobacteria occupy various ecological niches and can be isolated from soil, tap water and ground water. Several cause diseases in humans and animals. To get deeper insight into our understanding of mycobacterial evolution focusing on tRNA and non-coding (nc)RNA, we conducted a comparative genome analysis of Mycobacterium mucogenicum (Mmuc) and Mycobacterium neoaurum (Mneo) clade members.Results: Genome sizes for Mmuc- and Mneo-clade members vary between 5.4 and 6.5 Mbps with the complete Mmuc(T) (type strain) genome encompassing 6.1 Mbp. The number of tRNA genes range between 46 and 79 (including one pseudo tRNA gene) with 39 tRNA genes common among the members of these clades, while additional tRNA genes were probably acquired through horizontal gene transfer. Selected tRNAs and ncRNAs (RNase P RNA, tmRNA, 4.5S RNA, Ms1 RNA and 6C RNA) are expressed, and the levels for several of these are higher in stationary phase compared to exponentially growing cells. The rare tRNA(Ile)TAT isoacceptor and two for mycobacteria novel ncRNAs: the Lactobacillales-derived GOLLD RNA and a homolog to the antisense Salmonella typhimurium phage Sar RNA, were shown to be present and expressed in certain Mmuc-clade members.Conclusions: Phages, IS elements, horizontally transferred tRNA gene clusters, and phage-derived ncRNAs appears to have influenced the evolution of the Mmuc- and Mneo-clades. While the number of predicted coding sequences correlates with genome size, the number of tRNA coding genes does not. The majority of the tRNA genes in mycobacteria are transcribed mainly from single genes and the levels of certain ncRNAs, including RNase P RNA (essential for the processing of tRNAs), are higher at stationary phase compared to exponentially growing cells. We provide supporting evidence that Ms1 RNA represents a mycobacterial 6S RNA variant. The evolutionary routes for the ncRNAs RNase P RNA, tmRNA and Ms1 RNA are different from that of the core genes.
|
|
| 3. |
- Behra, Phani Rama Krishna
(författare)
-
Comparative genomics of the genus Mycobacterium : Genome evolution, phylogeny and diversity
- 2022
-
Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The genus Mycobacterium includes more than 190 species, and many cause severe diseases such as tuberculosis and leprosy. According to the "World Health Organization", in year 2019 alone, 10 million people developed TB, and 1.4 million died. TB had been in decline in developed countries, but made its reappearance as an opportunistic pathogen targeting immuno-compromised AIDS victims. Also, non-tuberculosis mycobacteria (NTM) infections have emerged as a major infectious agent in recent times. NTM occupy diverse ecological niches and can be isolated from soil, tap water, and groundwater. This thesis has investigated the Mycobacterium species from a genomic perspective, focusing on the biology of virulence factors, mobile genetic elements, tRNAs, and non-coding RNAs and their evolutionary distribution and possible relationship with phenotypic diversity. As part of this study, we have sequenced 153 mycobacterial genomes, including type strains, environmental samples, isolates from hospital patients, infected fish, and outbreak samples in an animal facility at Uppsala University. We have provided a phylogenetic tree based on 387 (and 56) core genes covering most species (244 genomes) constituting the Mycobacterium genus. The core gene phylogeny resulted in 33 clades. Subsequently, we have covered different clade groups, such as, M. marinum, M. mucogenicum, M. chelonae and M. chlorophenolicum and investigated the NTM clade-specific genome diversity and evolution. Our examination of non-coding genes showed that the total number of tRNA genes per species varies between 42 and 90. Among the species with more than 50 tRNAs, additional tRNA genes are likely acquired through horizontal gene transfer (HGT), as supported by the presence of closely linked HNH endonuclease gene and GOLLD RNA. We have explored the presence of selenocysteine utility and the gene for selenoprotein "formate dehydrogenase" among 244 mycobacterial genomes. For the M. chlorophenolicum clade, we have explored genes with a role in the bioremediation process. Comparative genomics of M. marinum and M. chelonae clade groups suggest new clusters or subspecies. Mutational hotspots are relatively higher in M. marinum compared to that in M. tuberculosis and M. salmoniphilum. Relatively higher number of hotspots in M. marinum is likely related to its ability to occupy different ecological niches. Finally, the thesis uncovered IS elements, phage sequences, plasmids, tRNA, and ncRNA contributing to mycobacterial evolution.
|
|
| 4. |
- Behra, Phani Rama Krishna, et al.
(författare)
-
Extended insight into the Mycobacterium chelonae-abscessus complex through whole genome sequencing of Mycobacterium salmoniphilum outbreak and Mycobacterium salmoniphilum-like strains
- 2019
-
Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 9
-
Tidskriftsartikel (refereegranskat)abstract
- Members of the Mycobacterium chelonae-abscessus complex (MCAC) are close to the mycobacterial ancestor and includes both human, animal and fish pathogens. We present the genomes of 14 members of this complex: the complete genomes of Mycobacterium salmoniphilum and Mycobacterium chelonae type strains, seven M. salmoniphilum isolates, and five M. salmoniphilum-like strains including strains isolated during an outbreak in an animal facility at Uppsala University. Average nucleotide identity (ANI) analysis and core gene phylogeny revealed that the M. salmoniphilum-like strains are variants of the human pathogen Mycobacterium franklinii and phylogenetically close to Mycobacterium abscessus. Our data further suggested that M. salmoniphilum separates into three branches named group I, II and III with the M. salmoniphilum type strain belonging to group II. Among predicted virulence factors, the presence of phospholipase C (plcC), which is a major virulence factor that makes M. abscessus highly cytotoxic to mouse macrophages, and that M. franklinii originally was isolated from infected humans make it plausible that the outbreak in the animal facility was caused by a M. salmoniphilum-like strain. Interestingly, M. salmoniphilum-like was isolated from tap water suggesting that it can be present in the environment. Moreover, we predicted the presence of mutational hotspots in the M. salmoniphilum isolates and 26% of these hotspots overlap with genes categorized as having roles in virulence, disease and defense. We also provide data about key genes involved in transcription and translation such as sigma factor, ribosomal protein and tRNA genes.
|
|
| 5. |
- Behra, Phani Rama Krishna, et al.
(författare)
-
Insight into the biology of Mycobacterium mucogenicum and Mycobacterium neoaurum Glade members
- 2019
-
Ingår i: Scientific Reports. - : NATURE PUBLISHING GROUP. - 2045-2322. ; 9
-
Tidskriftsartikel (refereegranskat)abstract
- Nontuberculous mycobacteria, NTM, are of growing concern and among these members of the Mycobacterium mucogenicum (Mmuc) and Mycobacterium neoaurum (Mneo) clades can cause infections in humans and they are resistant to first-line anti-tuberculosis drugs. They can be isolated from different ecological niches such as soil, tap water and ground water. Mycobacteria, such as Mmuc and Mneo, are classified as rapid growing mycobacteria, RGM, while the most familiar, Mycobacterium tuberculosis, belongs to the slow growing mycobacteria, SGM. Modern "omics" approaches have provided new insights into our understanding of the biology and evolution of this group of bacteria. Here we present comparative genomics data for seventeen NTM of which sixteen belong to the Mmuc- and Mneo-clades. Focusing on virulence genes, including genes encoding sigma/anti-sigma factors, serine threonine protein kinases (STPK), type VII (ESX genes) secretion systems and mammalian cell entry (Mce) factors we provide insight into their presence as well as phylogenetic relationship in the case of the sigma/anti-sigma factors and STPKs. Our data further suggest that these NTM lack ESX-5 and Mce2 genes, which are known to affect virulence. In this context, Mmuc- and Mneo-clade members lack several of the genes in the glycopeptidolipid (GLP) locus, which have roles in colony morphotype appearance and virulence. For the M. mucogenicum type strain, Mmuc(T), we provide RNASeq data focusing on mRNA levels for sigma factors, STPK, ESX proteins and Mce proteins. These data are discussed and compared to in particular the SGM and fish pathogen Mycobacterium marinum. Finally, we provide insight into as to why members of the Mmuc- and Mneo-clades show resistance to rifampin and isoniazid, and why Mmuc(T) forms a rough colony morphotype.
|
|
| 6. |
- Behra, Phani Rama Krishna, et al.
(författare)
-
Insight into the presence of selenocysteine among mycobacteria
-
Annan publikation (populärvet., debatt m.m.)abstract
- The Mycobacterium genus includes more than 190 species and occupies diverse ecological niches, and mycobacteria can be isolated from soil, water, humans and animals. Some are nonpathogenic and environmental, whereas others cause severe diseases both in humans and animals e.g. tuberculosis (TB) and leprosy. The 21st amino acid SelenoCysteine (SeC) is present in all three domains of life. In some mRNAs, an inframe stop codon UGA and a structural element, referred to as SECIS, are recognized by the tRNASeC-SelB-GTP complex resulting in SeC insertion during translation. Proteins having SeC are known as selenoproteins and some are involved in redox reactions and as such having roles in cell physiology. So far, only 20% of sequenced bacteria are known to incorporate selenocysteine. To study the presence of selenocysteine among mycobacteria we analysed 244 mycobacterial genomes for the presence of the selenocysteine machinery genes and selenoproteins. Our results suggested that more than 40% of the analysed mycobacterial genomes contain SeC related genes. Genes for the SeC machinery (selA, selB, selC and selD) are distributed evenly among slow and rapid growing mycobacteria and we identified only one selenoprotein, formate dehydrogenes, present in mycobacteria. Together our data expand our understanding of the selenocysteine metabolism among mycobacteria.
|
|
| 7. |
- Das, Sarbashis, et al.
(författare)
-
Characterization of three Mycobacterium spp. with potential use in bioremediation by genome sequencing and comparative genomics
- 2015
-
Ingår i: Genome Biology and Evolution. - : Oxford University Press (OUP). - 1759-6653. ; 7:7, s. 1871-1886
-
Tidskriftsartikel (refereegranskat)abstract
- We provide the genome sequences of the type strains of the polychlorophenol-degrading Mycobacterium chlorophenolicum (DSM43826), the degrader of chlorinated aliphatics Mycobacterium chubuense (DSM44219) and Mycobacterium obuense (DSM44075) that has been tested for use in cancer immunotherapy. The genome sizes of M. chlorophenolicum, M. chubuense and M. obuense are 6.93, 5.95 and 5.58 Mbps with GC-contents of 68.4, 69.2 and 67.9%, respectively. Comparative genomic analysis revealed that 3254 genes are common and we predicted approximately 250 genes acquired through horizontal gene transfer from different sources including proteobacteria. The data also showed that the biodegrading Mycobacterium spp. NBB4, also referred to as M. chubuense NBB4, is distantly related to the M. chubuense type strain and should be considered as a separate species, we suggest it to be named M. ethylenense NBB4. Among different categories we identified genes with potential roles in: biodegradation of aromatic compounds, and copper homeostasis. These are the first non-pathogenic Mycobacterium spp. found harboring genes involved in copper homeostasis. These findings would therefore provide insight into the role of this group of Mycobacterium spp. in bioremediation as well as the evolution of copper homeostasis within the Mycobacterium genus.
|
|
| 8. |
- Das, Sarbashis, et al.
(författare)
-
Extensive genomic diversity among Mycobacterium marinum strains revealed by whole genome sequencing
- 2018
-
Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 8
-
Tidskriftsartikel (refereegranskat)abstract
- Mycobacterium marinum is the causative agent for the tuberculosis-like disease mycobacteriosis in fish and skin lesions in humans. Ubiquitous in its geographical distribution, M. marinum is known to occupy diverse fish as hosts. However, information about its genomic diversity is limited. Here, we provide the genome sequences for 15 M. marinum strains isolated from infected humans and fish. Comparative genomic analysis of these and four available genomes of the M. marinum strains M, E11, MB2 and Europe reveal high genomic diversity among the strains, leading to the conclusion that M. marinum should be divided into two different clusters, the "M"- and the "Aronson"-type. We suggest that these two clusters should be considered to represent two M. marinum subspecies. Our data also show that the M. marinum pan-genome for both groups is open and expanding and we provide data showing high number of mutational hotspots in M. marinum relative to other mycobacteria such as Mycobacterium tuberculosis. This high genomic diversity might be related to the ability of M. marinum to occupy different ecological niches.
|
|
| 9. |
- Das, Sarbashis, et al.
(författare)
-
The Mycobacterium phlei Genome : Expectations and Surprises
- 2016
-
Ingår i: Genome Biology and Evolution. - : Oxford University Press (OUP). - 1759-6653. ; 8:4, s. 975-985
-
Tidskriftsartikel (refereegranskat)abstract
- Mycobacterium phlei, a nontuberculosis mycobacterial species, was first described in 1898-1899. We present the complete genome sequence for the IV, phlei CCUG21000(T) type strain and the draft genomes for four additional strains. The genome size for all five is 5.3 Mb with 69.4% Guanine-Cytosine content. This is approximate to 0.35 Mbp smaller than the previously reported M. phlei RIVM draft genome. The size difference is attributed partly to large bacteriophage sequence fragments in the M. phlei RIVM genome. Comparative analysis revealed the following: 1) A CRISPR system similar to Type 1E (cas3) in M. phiei RIVM; 2) genes involved in polyamine metabolism and transport (potAD, potT) that are absent in other mycobacteria, and 3) strain specific variations in the number of sigma-factor genes. Moreover, M. phlei has as many as 82 mce (mammalian cell entry) homologs and many of the horizontally acquired genes in M. phlei are present in other environmental bacteria including mycobacteria that share similar habitat. Phylogenetic analysis based on 693 Mycobacterium core genes present in all complete mycobacterial genomes suggested that its closest neighbor is Mycobacterium smegmatis JS623 and Mycobacterium rhodesiae NBB3, while it is more distant to M. smegmatis mc2 155.
|
|
| 10. |
- Pettersson, B. M. Fredrik, et al.
(författare)
-
Comparative Sigma Factor-mRNA Levels in Mycobacterium marinum under Stress Conditions and during Host Infection
- 2015
-
Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 10:10
-
Tidskriftsartikel (refereegranskat)abstract
- We have used RNASeq and qRT-PCR to study mRNA levels for all s-factors in different Mycobacterium marinum strains under various growth and stress conditions. We also studied their levels in M. marinum from infected fish and mosquito larvae. The annotated s-factors were expressed and transcripts varied in relation to growth and stress conditions. Some were highly abundant such as sigA, sigB, sigC, sigD, sigE and sigH while others were not. The s-factor mRNA profiles were similar after heat stress, during infection of fish and mosquito larvae. The similarity also applies to some of the known heat shock genes such as the a-crystallin gene. Therefore, it seems probable that the physiological state of M. marinum is similar when exposed to these different conditions. Moreover, the mosquito larvae data suggest that this is the state that the fish encounter when infected, at least with respect to s-factor mRNA levels. Comparative genomic analysis of s-factor gene localizations in three M. marinum strains and Mycobacterium tuberculosis H37Rv revealed chromosomal rearrangements that changed the localization of especially sigA, sigB, sigD, sigE, sigF and sigJ after the divergence of these two species. This may explain the variation in species-specific expression upon exposure to different growth conditions.
|
|
| 11. |
- Ramesh, Malavika, et al.
(författare)
-
Age-dependent pleomorphism in Mycobacterium monacense cultures
-
Annan publikation (populärvet., debatt m.m.)abstract
- Changes in cell shape and pleomorphism have been shown to be an integral part of the mycobacterial life cycle, however, systematic investigations into its patterns of pleomorphic behaviour in connection with stages or conditions of growth is scarce. We have studied the complete growth-cycle of Mycobacterium monacense cultures, a Non-Tuberculous Mycobacterium (NTM), in solid as well as liquid media. We provide data showing changes in cell shape from rod to coccoid and occurrence of refractive cells ranging from Phase Grey to phase Bright (PGB) in appearance upon ageing. Data further showed that changes in cell shape observed under the microscope could be correlated to the biphasic nature of the growth curves for M. monacense (as well as the NTM Mycobacterium boenickei) as measured by the absorbance of liquid cultures. Using the complete M. monacense genome we identified genes involved in cell morphology, and transcriptome analyses at different stages of growth revealed changes in their mRNA levels. One gene of interest, dnaK_3, that showed strong upregulation during stationary phase, was identified as an MreB-like homolog based on the protein domain architecture. Exogenous overexpression of M. monacense dnaK_3 in Mycobacterium marinum resulted in morphological changes with an impact on the frequency of occurrence of PGB cells. However, the introduction of an anti-sense "gene" targeting M. marinum dnaK_3 did not show such effects. Using dnaK_3-lacZ reporter constructs we provide data that these differences could be attributed to differences in the regulation of dnaK_3 in the two species. Together, this suggests that although its regulation may vary between mycobacterial species, dnaK_3 might be involved in the mechanism influencing mycobacterial cell shape.
|
|
| 12. |
- Ramesh, Malavika, et al.
(författare)
-
Age-dependent pleomorphism in Mycobacterium monacense cultures
-
Annan publikation (populärvet., debatt m.m.)abstract
- Changes in cell shape and pleomorphism have been shown to be an integral part of the mycobacterial life cycle, however, systematic investigations into its patterns of pleomorphic behaviour in connection with stages or conditions of growth is scarce. We have studied the complete growth-cycle of Mycobacterium monacense cultures, a Non-Tuberculous Mycobacterium (NTM), in solid as well as liquid media. We provide data showing changes in cell shape from rod to coccoid and occurrence of refractive cells ranging from Phase Grey to phase Bright (PGB) in appearance upon ageing. Data further showed that changes in cell shape observed under the microscope could be correlated to the biphasic nature of the growth curves for M. monacense (as well as the NTM Mycobacterium boenickei) as measured by the absorbance of liquid cultures. Using the complete M. monacense genome we identified genes involved in cell morphology, and transcriptome analyses at different stages of growth revealed changes in their mRNA levels. One gene of interest, dnaK_3, that showed strong upregulation during stationary phase, was identified as an MreB-like homolog based on the protein domain architecture. Exogenous overexpression of M. monacense dnaK_3 in Mycobacterium marinum resulted in morphological changes with an impact on the frequency of occurrence of PGB cells. However, the introduction of an anti-sense "gene" targeting M. marinum dnaK_3 did not show such effects. Using dnaK_3-lacZ reporter constructs we provide data that these differences could be attributed to differences in the regulation of dnaK_3 in the two species. Together, this suggests that although its regulation may vary between mycobacterial species, dnaK_3 might be involved in the mechanism influencing mycobacterial cell shape.
|
|
| 13. |
- Ramesh, Malavika, et al.
(författare)
-
Branching morphology in non-tuberculous mycobacteria Mycobacterium senegalense and Mycobacterium malmoense
-
Annan publikation (populärvet., debatt m.m.)abstract
- Understanding mycobacterial growth cycle has been a challenging topic ever since the identification of Mycobacterium tuberculosis (Mtb) as the causal agent of Tuberculosis. Diverse metabolic pathways, changes in cell shape and/or size under various growth and environmental conditions, dormancy and virulence are some of the major factors that have made mycobacterial studies complex and challenging. Over the years, many mycobacterial species classified as Non-Tuberculous Mycobacteria (NTM) have been isolated from humans, animals and identified as pathogens. In this study, we have observed and described the cell morphology of two NTMs that showed branching/filamentous growth. Mycobacterium senegalense (Msen), causative agent of bovine farcy showed extensive branching with spore-like phase grey and phase bright (PGB) structures similar to the ones observed in a slow-growing NTM Mycobacterium malmoense (Mmal), known to cause TB-like lung infections in humans. Global transcriptome analyses of various genes is suggestive of pathways and regulations that may be responsible for the pleiomorphsim in general and specifically for branching in Msen and Mmal. One such gene that was analysed was the wag31, which has been shown to localise preferably at branch points.
|
|
| 14. |
- Ramesh, Malavika, et al.
(författare)
-
Branching morphology in the non-tuberculous mycobacteria Mycobacterium senegalense and Mycobacterium malmoense
-
Annan publikation (populärvet., debatt m.m.)abstract
- Understanding mycobacterial growth cycle has been a challenging topic ever since the identification of Mycobacterium tuberculosis (Mtb) as the causal agent of Tuberculosis. Diverse metabolism, changes in cell shape and/or size under various growth and environmental conditions, dormancy and virulence are some of the major factors that has posed mycobacterial studies as very challenging. Over the years, many mycobacterial species classified as Non-Tuberculous Mycobacteria (NTM) have been isolated from humans, animals and identified as pathogens. In this study, we have observed and described the cell morphology of two NTMs that showed branching/filamentous growth. Mycobacterium senegalense (Msen), causative agent of bovine farcy showed extensive branching with spore-like phase grey and phase bright (PGB) structures similar to the ones observed in a slow-growing NTM Mycobacterium malmoense (Mmal), known to cause TB-like lung infections in humans. Global transcriptome analyses of various genes is suggestive of pathways and regulations that may be responsible for the pleiomorphsim in general and specifically for branching in Msen and Mmal. One such gene that was analysed was the wag31, which has been shown to localise preferably at branch points.
|
|
| 15. |
- Ramesh, Malavika, et al.
(författare)
-
Intracellular localization of the mycobacterial stressosome complex
- 2021
-
Ingår i: Scientific Reports. - : Springer Nature. - 2045-2322. ; 11:1
-
Tidskriftsartikel (refereegranskat)abstract
- Microorganisms survive stresses by alternating the expression of genes suitable for surviving the immediate and present danger and eventually adapt to new conditions. Many bacteria have evolved a multiprotein "molecular machinery" designated the "Stressosome" that integrates different stress signals and activates alternative sigma factors for appropriate downstream responses. We and others have identified orthologs of some of the Bacillus subtilis stressosome components, RsbR, RsbS, RsbT and RsbUVW in several mycobacteria and we have previously reported mutual interactions among the stressosome components RsbR, RsbS, RsbT and RsbUVW from Mycobacterium marinum. Here we provide evidence that "STAS" domains of both RsbR and RsbS are important for establishing the interaction and thus critical for stressosome assembly. Fluorescence microscopy further suggested co-localization of RsbR and RsbS in multiprotein complexes visible as co-localized fluorescent foci distributed at scattered locations in the M. marinum cytoplasm; the number, intensity and distribution of such foci changed in cells under stressed conditions. Finally, we provide bioinformatics data that 17 (of 244) mycobacteria, which lack the RsbRST genes, carry homologs of Bacillus cereus genes rsbK and rsbM indicating the existence of alternative σF activation pathways among mycobacteria.
|
|
| 16. |
- Ramesh, Malavika, et al.
(författare)
-
Pleiomorphism and gene expression profile of SGM Mycobacterium marinum and RGM Mycobacterium monacense under microaerobic condition
-
Annan publikation (populärvet., debatt m.m.)abstract
- Microaerobic condition is often studied in relation to pathogenesis of mycobacteria as it mimics the environment inside the lungs of an infected TB patient. Adaptation to a dormant state by Mycobacterium tuberculosis (Mtb) help the pathogen to persist within the host system and cause latent infections. Hypoxia, also referred to as microaerobic condition, is one of the major factors inducing dormancy in Mtb. Studies have characterised dormancy and latency by certain features such as inability to replicate, low metabolic activity, and expression of various genetic markers such as expression genes belonging to the Dos regulon (dormancy survival regulon). In addition to the mRNA profiles of various dormancy associated genes, this study also shows the morphological changes observed in the slow growing (SGM) Mycobacterium marinum and the rapid growing (RGM) Mycobacterium monacense. The observed morphological changes refers to increasing frequencies of refractive spore-like Phase Grey and phase Bright (PGB) cells in mycobacterial cultures when subjected to microaerobic condition. Similar observations with respect to PGB cells has also been detected in Mtb cultures. Together this suggests that changes in cell morphology under microaerobic condition occurs in mycobacteria irrespective of the species. Our microscopic and RNASeq data further suggested that sigB and the mreB-like homolog dnaK_3 both influence cell shape upon depletion of oxygen.
|
|
| 17. |
- Ramesh, Malavika, et al.
(författare)
-
Pleomorphism and gene expression profile of SGM Mycobacterium marinum and RGM Mycobacterium monacense under microaerobic condition
-
Annan publikation (populärvet., debatt m.m.)abstract
- Microaerobic condition is often studied in relation to pathogenesis of mycobacteria as it mimics the environment inside the lungs of an infected TB patient. Adaptation to a dormant state by Mycobacterium tuberculosis (Mtb) help the pathogen to persist within the host system and cause latent infections. Hypoxia, also referred to as microaerobic condition, is one of the major factors inducing dormancy in Mtb. Studies have characterised dormancy and latency by certain features such as inability to replicate, low metabolic activity, and expression of various genetic markers such as expression genes belonging to the Dos regulon (dormancy survival regulon). In addition to the mRNA profiles of various dormancy associated genes, this study also shows the morphological changes observed in the slow growing (SGM) Mycobacterium marinum and the rapid growing (RGM) Mycobacterium monacense. The observed morphological changes refers to increasing frequencies of refractive spore-like Phase Grey and phase Bright (PGB) cells in mycobacterial cultures when subjected to microaerobic condition. Similar observations with respect to PGB cells has also been detected in Mtb cultures. Together this suggests that changes in cell morphology under microaerobic condition occurs in mycobacteria irrespective of the species. Our microscopic and RNASeq data further suggested that sigB and the mreB-like homolog dnaK_3 both influence cell shape upon depletion of oxygen.
|
|
| 18. |
- Singla, Mamta, et al.
(författare)
-
Sub-operon promoter arrangement of disA facilitates c-di-AMP homeostasis and selective stress responses in Mycobacterium smegmatis
- 2023
-
Ingår i: Journal of Biosciences. - : Springer Nature. - 0250-5991 .- 0973-7138. ; 48:3
-
Tidskriftsartikel (refereegranskat)abstract
- Bacterial second messenger signaling often plays an important role in cellular physiology. In this study, we have attempted to understand how c-di-AMP synthesis and degradation are transcriptionally regulated in Mycobacterium smegmatis. We found that although a c-di-AMP synthesis gene, disA, exists in a multi-gene operon, a sub-operon promoter arrangement facilitates disA gene expression under normal conditions to maintain intracellular c-di-AMP concentration and is induced further during certain stress adaptations. Individual gene-specific promoters also play a key role under various genotoxic stress conditions to shut down c-di-AMP synthesis, which could otherwise be detrimental for cells. Further, we learned that a high c-di-AMP concentration plays a role in the autoregulation of the disA promoter to limit intracellular c-di-AMP concentration. This study was helpful to understand how c-di-AMP synthesis is regulated under normal and stress conditions linked to its physiological relevance in M. smegmatis.
|
|
| 19. |
- Suarez, Rudy, et al.
(författare)
-
Whole-Genome sequencing and comparative genomics of Mycobacterium spp. from farmed Atlantic and coho salmon in Chile
- 2021
-
Ingår i: Antonie van Leeuwenhoek. International Journal of General and Molecular Microbiology. - : Springer. - 0003-6072 .- 1572-9699. ; 114:9, s. 1323-1336
-
Tidskriftsartikel (refereegranskat)abstract
- Several members of the Mycobacterium genus cause invasive infections in humans and animals. According to a recent phylogenetic analysis, some strains of Mycobacterium salmoniphilum (Msal), which are the main culprit in bacterial outbreaks in freshwater fish aquaculture, have been assigned to a separate branch containing Mycobacterium franklinii (Mfra), another species that causes infections in humans. However, this genus is little studied in an aquaculture context. Here, we isolated four Mycobacterium spp. strains from freshwater cultures of Atlantic and coho salmon in Chile and performed whole-genome sequencing for deep genomic characterization. In addition, we described the gross pathology and histopathology of the outbreaks. Several bioinformatic analyses were performed using the genomes of these four Mycobacterium isolates in conjunction with those of Msal strains, four Msal-like strains, and one Mfra strains, plus 17 other publicly available Mycobacterium genomes. We found that three isolates are clustered into the Msal branch, whereas one isolate clustered with the Mfra/Msal-like strains. We further evaluated the presence of virulence and antimicrobial resistance genes and observed that the four isolates were closely related to the Msal and Msal-like taxa and carried several antimicrobial resistance and virulence genes that are similar to those of other pathogenic members of the Mycobacterium clade. Altogether, our characterization Msal and Msal-like presented here shed new light on the basis of mycobacteriosis provides quantitative evidence that Mycobacterium strains are a potential risk for aquaculture asetiological agents of emerging diseases, and highlight their biological scopes in the aquaculture industry.
|
|
| 20. |
- Ye, Weihua, et al.
(författare)
-
Folded Alpha Helical Putative New Proteins from Apilactobacillus kunkeei
- 2024
-
Ingår i: Journal of Molecular Biology. - : Elsevier. - 0022-2836 .- 1089-8638. ; 436:6
-
Tidskriftsartikel (refereegranskat)abstract
- The emergence of new proteins is a central question in biology. Most tertiary protein folds known to date appear to have an ancient origin, but it is clear from bioinformatic analyses that new proteins continuously emerge in all organismal groups. However, there is a paucity of experimental data on new proteins regarding their structure and biophysical properties. We performed a detailed phylogenetic analysis and identified 48 putative open reading frames in the honeybee -associated bacterium Apilactobacillus kunkeei for which no or few homologs could be identified in closely -related species, suggesting that they could be relatively new on an evolutionary time scale and represent recently evolved proteins. Using circular dichroism-, fluorescence- and nuclear magnetic resonance (NMR) spectroscopy we investigated six of these proteins and show that they are not intrinsically disordered, but populate alpha -helical dominated folded states with relatively low thermodynamic stability (0-3 kcal/mol). The NMR and biophysical data demonstrate that small new proteins readily adopt simple folded conformations suggesting that more complex tertiary structures can be continuously re -invented during evolution by fusion of such simple secondary structure elements. These findings have implications for the general view on protein evolution, where de novo emergence of folded proteins may be a common event.
|
|
