| 1. |
- Nedellec, R, et al.
(författare)
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Virus Entry via the Alternative Coreceptors CCR3 and FPRL1 Differs by Human Immunodeficiency Virus Type 1 Subtype.
- 2009
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Ingår i: Journal of Virology. - 1098-5514. ; 83, s. 8353-8363
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Tidskriftsartikel (refereegranskat)abstract
- HIV-1 infects target cells by binding to CD4 and a chemokine receptor, most commonly CCR5. CXCR4 is a frequent alternative coreceptor (CoR) in subtype B and D HIV-1 infection, but the importance of many other alternative CoR remains elusive. We have analyzed HIV-1 envelope proteins (Env) from 66 individuals infected with the major subtypes of HIV-1 to determine if virus entry into highly permissive NP-2 cell lines expressing most known alternative CoR differed by HIV-1 subtype. We also performed linear regression analysis to determine if virus entry via the major CoR CCR5 correlated with use of any alternative CoR, and if this correlation differed by subtype. Virus pseudotyped with subtype B Env showed robust entry via CCR3 that was highly correlated with CCR5 entry efficiency. By contrast, viruses pseudotyped with subtype A and C Env were able to use the recently described alternative CoR FPRL1 more efficiently than CCR3, and use of FPRL1 was correlated with CCR5 entry. Subtype D Env was unable to use either CCR3 or FPRL1 efficiently, a unique pattern of alternative CoR use. These results suggest that each subtype of circulating HIV-1 may be subject to somewhat different selective pressures for Env-mediated entry into target cells, and suggest that CCR3 may be used as a surrogate CoR by subtype B while FPRL1 may be used as a surrogate CoR by subtype A and C. These data may provide insight into development of resistance to CCR5-targeted entry inhibitors and alternative entry pathways for each HIV-1 subtype.
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| 2. |
- Beven, Keith J., et al.
(författare)
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GLUE : 20 years on
- 2014
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Ingår i: Hydrological Processes. - : Wiley. - 0885-6087 .- 1099-1085. ; 28:24, s. 5897-5918
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Forskningsöversikt (refereegranskat)abstract
- This paper reviews the use of the Generalized Likelihood Uncertainty Estimation (GLUE) methodology in the 20 years since the paper by Beven and Binley in Hydrological Processes in (1992), which is now one of the most highly cited papers in hydrology. The original conception, the on-going controversy it has generated, the nature of different sources of uncertainty and the meaning of the GLUE prediction uncertainty bounds are discussed. The hydrological, rather than statistical, arguments about the nature of model and data errors and uncertainties that are the basis for GLUE are emphasized. The application of the Institute of Hydrology distributed model to the Gwy catchment at Plynlimon presented in the original paper is revisited, using a larger sample of models, a wider range of likelihood evaluations and new visualization techniques. It is concluded that there are good reasons to reject this model for that data set. This is a positive result in a research environment in that it requires improved models or data to be made available. In practice, there may be ethical issues of using outputs from models for which there is evidence for model rejection in decision making. Finally, some suggestions for what is needed in the next 20 years are provided.
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| 3. |
- Rossi, M., et al.
(författare)
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Intricacies in the interpretation of vertical radar profiling caused by borehole effects
- 2017
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Ingår i: Conference Proceedings, 23rd European Meeting of Environmental and Engineering Geophysics. - Netherlands : EAGE Publications BV. - 9789462822238 ; 2017, s. 1-5
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Konferensbidrag (refereegranskat)abstract
- Estimation of hydraulic parameters of the vadose zone is a relevant issue in hydrological characterization and flow model calibration. Two borehole Ground Penetrating Radar (GPR) techniques are discussed: Zero-Offset Profiling (ZOP) and Vertical Radar Profiling (VRP). The field case is representative of a very common situation in vadose zone characterization: Above the water table, the permittivity inside the air-filled borehole is significantly smaller than in the embedding soil. In this case, if the first-arrivals are picked and the corresponding inversions are performed without a careful analysis, the recovered dielectric relative permittivity (år) profiles are in large disagreement. The presented analysis of VRP synthetic and real datasets clarifies that the presence of the air-filled borehole may alter the propagation of electromagnetic waves, invalidating the comparison among VRP and ZOP first-arrivals. Once the borehole effects are accounted, the comparison between the ZOP and VRP år-profiles is more reasonable and reveals the different resolution of these techniques, focusing on the information that can be inferred for hydrological characterizations. Thus, VRP surveys in vadose zone must be accurately interpreted, as the electromagnetic waves may propagate via "guided" modes along the borehole.
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