| 1. |
- Björklund, Erik, et al.
(författare)
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Admission N-terminal pro-brain natriuretic peptide and its interaction with admission troponin T and ST segment resolution for early risk stratification in ST elevation myocardial infarction
- 2006
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Ingår i: Heart. - : BMJ Publishing Group. - 1468-201X .- 1355-6037. ; 92:6, s. 735-40
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: To assess the long term prognostic value of N-terminal pro-brain natriuretic peptide (NT-proBNP) on admission and its prognostic interaction with both admission troponin T (TnT) concentrations and resolution of ST segment elevation in fibrinolytic treated ST elevation myocardial infarction (STEMI). DESIGN AND SETTING: Substudy of the ASSENT (assessment of the safety and efficacy of a new thrombolytic) -2 and ASSENT-PLUS trials. PATIENTS: NT-proBNP and TnT concentrations were determined on admission in 782 patients. According to NT-proBNP concentrations, patients were divided into three groups: normal concentration (for patients < or = 65 years, < or = 184 ng/l and < or = 268 ng/l and for those > 65 years, < or = 269 ng/l and < or = 391 ng/l in men and women, respectively); higher than normal but less than the median concentration (742 ng/l); and above the median concentration. For TnT, a cut off of 0.1 microg/l was used. Of the 782 patients, 456 had ST segment resolution (< 50% or > or = 50%) at 60 minutes calculated from ST monitoring. MAIN OUTCOME MEASURES: All cause one year mortality. RESULTS: One year mortality increased stepwise according to increasing concentrations of NT-proBNP (3.4%, 6.5%, and 23.5%, respectively, p < 0.001). In receiver operating characteristic analysis, NT-proBNP strongly trended to be associated more with mortality than TnT and time to 50% ST resolution (area under the curve 0.81, 95% confidence interval (CI) 0.72 to 0.9, 0.67, 95% CI 0.56 to 0.79, and 0.66, 95% CI 0.56 to 0.77, respectively). In a multivariable analysis adjusted for baseline risk factors and TnT, both raised NT-proBNP and ST resolution < 50% were independently associated with higher one year mortality, whereas raised TnT contributed independently only before information on ST resolution was added to the model. CONCLUSION: Admission NT-proBNP is a strong independent predictor of mortality and gives, together with 50% ST resolution at 60 minutes, important prognostic information even after adjustment for TnT and baseline characteristics in STEMI.
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| 2. |
- Björklund, Erik, et al.
(författare)
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Admission Troponin T and measurement of ST-segment resolution at 60 min improve early risk stratification in ST-elevation myocardial infarction
- 2004
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Ingår i: Eur Heart J. - : Oxford University Press (OUP). - 0195-668X .- 1522-9645. ; 25:2, s. 113-20
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Tidskriftsartikel (refereegranskat)abstract
- AIMS: The prognostic value of admission troponin T (tnT) levels and the resolution of the ST-segment elevation in ST-elevation myocardial infarction (STEMI) is well established. However, the combination of these two early available markers for predicting risk has not been evaluated. METHODS AND RESULTS: We evaluated 516 patients with fibrinolytic treated STEMI from the ASSENT-2 and ASSENT-PLUS studies, which had both admission tnT and ST-monitoring available. We used a prospectively defined cut-off value of tnT of 0.1microg/l. For ST-segment resolution, a cut-off of 50% measured after 60min was used. Both a tnT >/=0.1microg/l (n=116) and ST-segment resolution <50% (n=301) were related to higher one-year mortality, 13% vs 4% (P<0.001) and 8.4% vs 2.8% (P=0.009), respectively. In a multivariate analysis ST-segment resolution was and tnT showed a strong trend to be independently related to mortality. The combination of both further improved risk stratification. The one-year mortality in the group with elevation of tnT and without ST-segment resolution compared to the group without tnT elevation and with ST-segment resolution was 18.2% vs 2.8% (P<0.001). CONCLUSIONS: Both tnT on admission and ST-segment resolution after 60min are strong predictors of one-year mortality. The combination of both gives additive early information about prognosis and further improves risk stratification.
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| 3. |
- Nåbo, Arne, 1958-, et al.
(författare)
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Battery-Swapping for Heavy Duty Vehicles : A Feasibility Study on Up-Scaling in Sweden
- 2024
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Rapporten fokuserar på den kommersiella genomförbarheten av ett batteribytessystem för tunga lastbilar i Sverige. Genom att studera affärsmodeller, kompatibilitet med svenska regelverk och integration i transportverksamheten har vi utforskat hur disruptiva teknologier, ekosystemeffekter och cirkularitet skulle kunna möjliggöra en snabb introduktion och uppskalning av ett batteribytessystem. Ett särskilt fokus har legat på Kina för att analysera statusen för batteribyten och de processer som har lett till den snabba utvecklingen och uppskalningen där. I Kina krävde batteribyte en ny affärsmodell där aktörer såsom energiproducenter, batteritillverkare och maskinindustri går i spetsen för utvecklingen och spridningen av batteribyten. Batteribyte är nu den dominerande tekniken för eldrivna lastbilar i Kina.Exempel på fördelar med batteribyte som förespråkas är att det bara tar några minuter att byta batteri, minskad investering för lastbilsägare, låg påverkan på det lokala elnätet och att fordons- och batterilivscykler separeras. En simuleringsstudie om batteribyte för tunga lastbilar i hamnverksamhet visar i denna rapport på tydliga fördelar jämfört med kabelladdning. Det finns dock ett par utmaningar med att införa batteribyte i Sverige. För det första finns det inga tydliga förespråkare för batteribyte inom industrin. Till exempel är de svenska och europeiska fordonstillverkarna tveksamma eftersom det utmanar deras nuvarande affärsmodell och att de kan ta rollen som grindvakter. För det andra omfattar inte de nuvarande standarderna och regelverken för fordon och energisystem i Sverige och i EU batteribyte. Rapporten tar också upp behovet av kunskap och utbildning av personer vid batteribytesstationer, samt vikten av social hållbarhet vid elektrifiering av tunga transporter.
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| 4. |
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| 5. |
- Alström, Per, et al.
(författare)
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The Arctic Warbler Phylloscopus borealis - three anciently separated cryptic species revealed
- 2011
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Ingår i: Ibis. - 0019-1019 .- 1474-919X. ; 153:2, s. 395-410
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Tidskriftsartikel (refereegranskat)abstract
- The Arctic Warbler Phylloscopus borealis breeds across the northern Palaearctic and northwestern-most Nearctic, from northern Scandinavia to Alaska, extending south to southern Japan, and winters in Southeast Asia, the Philippines and Indonesia. Several subspecies have been described based on subtle morphological characteristics, although the taxonomy varies considerably among different authors. A recent study (T. Saitoh et al. (2010) BMC Evol. Biol. 10: 35) identified three main mitochondrial DNA clades, corresponding to: (1) continental Eurasia and Alaska, (2) south Kamchatka, Sakhalin and northeast Hokkaido, and (3) most of Japan (Honshu, Shikoku, Kyushu). These three clades were estimated to have diverged during the late Pliocene to early Pleistocene (border at c. 2.6 million years ago). Differences in morphometrics have also been reported among members of the three clades (T. Saitoh et al. (2008) Ornithol. Sci. 7: 135-142). Here we analyse songs and calls from throughout the range of the Arctic Warbler, and conclude that these differ markedly and consistently among the populations representing the three mitochondrial clades. Kurile populations, for which no sequence data are available, are shown to belong to the second clade. To determine the correct application of available scientific names, mitochondrial DNA was sequenced from three name-bearing type specimens collected on migration or in the winter quarters. Based on the congruent variation in mitochondrial DNA, morphology and vocalizations, we propose that three species be recognized: Arctic Warbler Phylloscopus borealis (sensu stricto) (continental Eurasia and Alaska), Kamchatka Leaf Warbler Phylloscopus examinandus (Kamchatka (at least the southern part), Sakhalin, Hokkaido and Kurile Islands), and Japanese Leaf Warbler Phylloscopus xanthodryas (Japan except Hokkaido).
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| 6. |
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| 7. |
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| 8. |
- Annerbo, Maria, 1967-
(författare)
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Calcium Homeostasis in Patients with Graves' Disease
- 2016
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Patients with Graves´ Disease (GD) have a higher risk of developing more severe and prolonged hypocalcaemia after total thyroidectomy (TT) than patients who undergo surgery for benign atoxic goitre. Since TT is the most effective treatment for GD, it is crucial to identify mechanisms for postoperative hypocalcaemia. The aim of this thesis was to study the mechanisms of calcium metabolism in patients with GD.It is safe to operate on GD patients with TT. Results in Paper I showed fewer recurrences and equal complication rates compared to patients who underwent subtotal thyroidectomy (ST). The transient lowering of PTH seen in the hypocalcaemic patients was fully restored one month after surgery (Papers II and V).The calcium-sensing receptor (CaSR) is crucial for maintaining plasma calcium, and single nucleotide polymorphisms (SNPs) in the gene may alter the sensing function. Thus, we analysed SNPs in CaSR in GD patients (Paper II) and showed that they had a more left-shifted calcium-PTH set-point compared to controls, implicating higher sensitivity. This is also supported by the results in the group of postoperatively hypocalcaemic patients. They already had lower plasma calcium preoperatively (Papers II, IV and V) and lacked the T/G G/A G/C, a haplotype shown in Paper III to have a close relationship to higher p-calcium levels. Moreover, a lack of the T allele in rs1801725 was seen in the group of patients needing permanent treatment with calcium and vitamin D, i.e. > 12 months, (paper V).Patients who became hypocalcaemic (p-calcium < 2.00 mmol/L) on day one postoperatively, had lower preoperative levels of thyroid stimulating hormone (TSH) and higher levels of T3, this was also applied to the patient groups requiring temporary or permanent postoperative treatment (Papers II and V). In addition, hypocalcaemic patients treated for less than six months with anti-thyroid drugs had higher levels of bone metabolism markers CTX and P1NP than normocalcaemic patients (Paper V).In conclusion, the postoperative period of hypocalcaemia seen in patients with GD is a complex medical condition, caused by a combination of surgical trauma, different SNPs in CaSR, and high bone metabolism related to preoperative thyroid metabolism.
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| 9. |
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| 10. |
- Backman, Samuel, et al.
(författare)
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Global DNA Methylation Analysis Identifies Two Discrete clusters of Pheochromocytoma with Distinct Genomic and Genetic Alterations
- 2017
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7
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Tidskriftsartikel (refereegranskat)abstract
- Pheochromocytomas and paragangliomas (PPGLs) are rare and frequently heritable neural-crest derived tumours arising from the adrenal medulla or extra-adrenal chromaffin cells respectively. The majority of PPGL tumours are benign and do not recur with distant metastases. However, a sizeable fraction of these tumours secrete vasoactive catecholamines into the circulation causing a variety of symptoms including hypertension, palpitations and diaphoresis. The genetic landscape of PPGL has been well characterized and more than a dozen genes have been described as recurrently mutated. Recent studies of DNA-methylation have revealed distinct clusters of PPGL that share DNA methylation patterns and driver mutations, as well as identified potential biomarkers for malignancy. However, these findings have not been adequately validated in independent cohorts. In this study we use an array-based genome-wide approach to study the methylome of 39 PPGL and 4 normal adrenal medullae. We identified two distinct clusters of tumours characterized by different methylation patterns and different driver mutations. Moreover, we identify genes that are differentially methylated between tumour subcategories, and between tumours and normal tissue.
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| 11. |
- Backman, Samuel, et al.
(författare)
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RNA Sequencing Provides Novel Insights into the Transcriptome of Aldosterone Producing Adenomas
- 2019
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Ingår i: Scientific Reports. - : NATURE PUBLISHING GROUP. - 2045-2322. ; 9
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Tidskriftsartikel (refereegranskat)abstract
- Aldosterone producing adenomas (APAs) occur in the adrenal glands of around 30% of patients with primary aldosteronism, the most common form of secondary hypertension. Somatic mutations in KCNJ5, ATP1A1, ATP2B3, CACNA1D and CTNNB1 have been described in similar to 60% of these tumours. We subjected 15 aldosterone producing adenomas (13 with known mutations and two without) to RNA Sequencing and Whole Genome Sequencing (n = 2). All known mutations were detected in the RNA-Seq reads, and mutations in ATP2B3 (G123R) and CACNA1D (S410L) were discovered in the tumours without known mutations. Adenomas with CTNNB1 mutations showed a large number of differentially expressed genes (1360 compared to 106 and 75 for KCNJ5 and ATP1A1/ATP2B3 respectively) and clustered together in a hierarchical clustering analysis. RT-PCR in an extended cohort of 49 APAs confirmed higher expression of AFF3 and ISM1 in APAs with CTNNB1 mutations. Investigation of the expression of genes involved in proliferation and apoptosis revealed subtle differences between tumours with and without CTNNB1 mutations. Together our results consolidate the notion that CTNNB1 mutations characterize a distinct subgroup of APAs.
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| 12. |
- Beziat, Vivien, et al.
(författare)
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NK cell responses to cytomegalovirus infection lead to stable imprints in the human KIR repertoire and involve activating KIRs
- 2013
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Ingår i: Blood. - : American Society of Hematology. - 0006-4971 .- 1528-0020. ; 121:14, s. 2678-2688
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Tidskriftsartikel (refereegranskat)abstract
- Human natural killer (NK) cells are functionally regulated by killer cell immunoglobulin-like receptors (KIRs) and their interactions with HLA class I molecules. As KIR expression in a given NK cell is genetically hard-wired, we hypothesized that KIR repertoire perturbations reflect expansions of unique NK-cell subsets and may be used to trace adaptation of the NK-cell compartment to virus infections. By determining the human KIR-ome at a single-cell level in more than 200 donors, we were able to analyze the magnitude of NK cell adaptation to virus infections in healthy individuals. Strikingly, infection with human cytomegalovirus (CMV), but not with other common herpesviruses, induced expansion and differentiation of KIR-expressing NK cells, visible as stable imprints in the repertoire. Education by inhibitory KIRs promoted the clonal-like expansion of NK cells, causing a bias for self-specific inhibitory KIRs. Furthermore, our data revealed a unique contribution of activating KIRs (KIR2DS4, KIR2DS2, or KIR3DS1), in addition to NKG2C, in the expansion of human NK cells. These results provide new insight into the diversity of KIR repertoire and its adaptation to virus infection, suggesting a role for both activating and inhibitory KIRs in immunity to CMV infection.
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| 13. |
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| 14. |
- Björklund, Anders, 1950-, et al.
(författare)
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Arbetsmarknaden
- 2014. - 4
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Bok (övrigt vetenskapligt/konstnärligt)
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| 15. |
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| 16. |
- Björklund, Anna, et al.
(författare)
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Energiplanering med strategisk miljöbedömning i Finspång
- 2007
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Denna rapport är en del av slutrapporteringen från två av projekten i programmet ”Miljöstrategiska verktyg”. Projekten är Utveckling av verktyg för strategisk miljöbedömning och medborgarmedverkan (MiSt-projekt 1) samt Scenariometoder i strategisk miljöbedömning (MiSt-projekt 7). I samarbete med Finspångs kommun har ett gemensamt projekt utvecklats för att ta fram en ny energiplan i kommunen. Programmet "Miljöstrategiska verktyg", MiSt, är ett tvärvetenskapligt forskningsprogram finansierat av Naturvårdsverket. Programmet leds från Blekinge Tekniska Högskola. I programmet studeras verktyg som kan underlätta miljöbedömning i strategiskt beslutsfattande på olika nivåer, från nationell till lokal. Förståelsen och basen för utveckling av verktyg och rekommendationer om verktyg i olika planerings- och beslutsprocesser baseras på empirisk forskning på fall inom flera sektorer. Målen för MiSt-programmet: • Kritisk undersökning av verktygens funktion • Teoribaserad förståelse av deras verkan • Utveckling av råd om effektiv användning av verktyg och kombinationer avverktyg
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| 17. |
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| 18. |
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| 19. |
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| 20. |
- Björklund, Camilla, et al.
(författare)
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Matematikkundervisning
- 2013
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- Denna boken är skriven av en grupp erfarna norska och svenska lärarutbildare i matematik. Boken bygger på utprövad erfarenhet - såväl egen som andras - och på aktuell, relevant forskning i matematikdidaktik. Texten väver samman matematik och matematikdidaktik, det vill säga ämnet som det undervisas i och frågor om hur ämnet kan läras och undervisas. Boken innehåller det som är absolut viktigast att få med sig i den grundläggande lärarutbildningen i matmatik.
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| 21. |
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| 22. |
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| 23. |
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| 24. |
- Björklund, Karin, 1979, et al.
(författare)
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Best Management Practices to Reduce Phthalate and Nonylphenol Loads in Urban Runoff
- 2008
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Ingår i: Proceedings of the International Conference on Urban Drainage, 1-5 September, Edinburgh, UK.
-
Konferensbidrag (refereegranskat)abstract
- Phthalates and nonylphenols, organic substances of widespread use, are believed to be ubiquitous in Swedish stormwater and urban sediments. Due to their reported hazardous properties, it is of great interest to identify effective best management practices (BMPs) to reduce the release of these substances to recipient waters. The stormwater-quality model SEWSYS was used to identify sources and quantify loads of phthalates and nonylphenols in two urban catchment areas. Based on the modelling results, BMPs were proposed, and their efficiencies evaluated. The SEWSYS simulations showed that discharges of phthalates and nonylphenols depend on catchment area characteristics: pollutants in a residential area originate mainly from building materials, whereas vehicles are the dominant source in a high-density traffic area. By replacing traditional building materials by phthalate and nonylphenol-free alternatives and by implementing congestion taxes to reduce traffic, the simulated pollutant loads could be reduced by more than 20% in the high-density traffic area. However, discharges from many diffuse sources cannot entirely be stopped, and a structural BMP, e.g. a sedimentation pond, is essential to further decrease pollutant loads in urban runoff. This study shows that SEWSYS can be a useful tool for selecting and evaluating adequate BMPs to reduce stormwater contamination.
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| 25. |
- Björklund, Karin, 1979, et al.
(författare)
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Källor till och flöden av ftalater och nonylfenoler i Stockholms dagvatten
- 2007
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- SEWSYS is a computer model designed for simulating fluxes of pollutants from its’ sources to the urban stormwater system. The model is able to simulate concentrations of substances in stormwater – thereby reducing the need for sampling and analyses of stormwater – as well as establishing the major sources of the pollutants in the urban environment. The aim of this work has been to identify the sources of some selected organic pollutants, adjusting SEWSYS to these new substances and simulating their fluxes in urban stormwater. The simulation results have then been used to investigate proper barriers to prevent these substances to reach the environment.The prioritised pollutants chosen to be studied were four phthalates – DBP, DEHP, DINP and DIDP – nonylphenols (NP) and their ethoxylates (NPE). Phthalates are plasticisers used in PVC (often as coil coating), paint and sealants. NP/E:s are surfactants used in a range of materials, such as concrete, paint and plastics. In order to add these pollutants into the SEWSYS model, the emission of each substance from each of the identified sources was scrutinised. Depending on the overall lack of data, assumptions and estimations were necessary to be made for the emission calculations.Two housing areas and one area dominated by traffic were selected for the modelling. Information on the prevalence of the different sources together with rainfall data were used for the simulation of stormwater runoff and pollution load. The model was calibrated with measured stormwater flows and volumes, as well as analysed concentrations of the pollutants in stormwater from the chosen housing and traffic areas. The traffic area showed high concentrations of phthalates in the stormwater samples. DIDP, and particularly DINP, showed the highest concentrations in most samples, both from the traffic area and the housing areas. The NP and NPE concentrations in most samples were close to or under the detection limits for the analytical methods used.The calibration of the model showed that the runoff module in SEWSYS is able to predict the runoff volume well. After recalculating and adjusting some of the emission factors, the concentrations of the pollutants were in the same magnitude as the measured values. The simulations showed that vehicles are the dominating source of the phthalates in the traffic area. Parking spaces and concrete are indicated to emit significant amounts of NP/E:s. In the housing areas the construction materials – such as plastic coated steel for roofing and cladding – were the most important sources of phthalates. Parking spaces tend to cause high pollution loads of NP/E:s.To reduce the load of organic pollutants in the environment a combination of barriers can be used – these barriers may include legislative measures to prohibit the use of a substance, recommendations to the consumers (not) to use specific construction materials or installing technical measures such as sedimentation facilities. A preliminary simulation show that by using construction materials free from phthalates or NP/E:s or reducing the traffic load, combined with a technical barrier such as a sedimentation pond, the pollution load from housing or traffic area is considerably decreased.
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| 26. |
- Björklund, Karin, 1979, et al.
(författare)
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Phthalates and nonylphenols in urban runoff: Occurrence, distribution and area emission factors
- 2009
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Ingår i: Science of the Total Environment. - : Elsevier BV. - 0048-9697 .- 1879-1026. ; 407:16, s. 4665-4672
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Tidskriftsartikel (refereegranskat)abstract
- The urban water system is believed to be an important sink for the nonpoint-source pollutants nonylphenols and phthalates. The presence of nonylphenols (NPs), nonylphenol ethoxylates (NPEOs), and eight phthalates was analyzed in urban stormwater and sediment from three catchment areas in Sweden. Emission loads for these substances were then calculated for a specific urban catchment area. In addition, substance distribution in road runoff passing through a sedimentation facility was modeled using a modified QWASI-model for chemical fate. High concentrations of DEHP, DIDP and DINP (≤ 48, 66 and 200 µg/g dw, respectively) as well as nonylphenol mono- and di-ethoxylate (6.6 and 20 µg/g dw, respectively) were found in the sediment. Aqueous concentrations of the pollutants varied considerably; branched NP was detected in concentrations up to 1.2 µg/L, whereas di(2-ethylhexyl) phthalate (DEHP), diisodecyl phthalate (DIDP), and diisononyl phthalate (DINP) were the most frequently detected phthalates in concentrations up to 5.0, 17 and 85 µg/L, respectively. The fate modeling demonstrated that predicted substance levels in water agreed well with measured levels, whereas the modeled sediment levels were underestimated. Calculation of catchment area emission factors from an urban highway environment revealed that as much as 2.1 kg of total phthalates and 200 g of NP and NPEOs may be emitted per hectare and year. The results indicate that all monitored phthalates, branched NPs and lower NPEOs are present in Swedish urban water systems. The long-chain phthalates DIDP and DINP are believed to occur at higher concentrations than other phthalates because of their higher environmental persistence and their increasing use in Sweden.
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| 27. |
- Björklund, Karin, 1979, et al.
(författare)
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Screening of Organic Contaminants in Urban Snow
- 2011
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Ingår i: Water Science and Technology. - : IWA Publishing. - 0273-1223 .- 1996-9732. ; 64:1, s. 206-213
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Tidskriftsartikel (refereegranskat)abstract
- Snowmelt is known to cause peak concentrations of pollutants, which may adversely affectreceiving water quality. High concentrations of metals and suspended solids in snow have beenreported, whereas studies on organic pollutants are rare. This study aims at investigating theoccurrence of anthropogenic organic compounds in urban snow in Gothenburg (Sweden). The mostfrequently detected organic pollutants in the collected snow samples were polycyclic aromatichydrocarbons (PAHs), high molecular-weight phthalates, 4-nonylphenol and 4-t-octylphenol.Brominated flame retardants and chlorinated paraffins were only sporadically detected. In severalsnow samples, the concentrations of specific PAHs, alkylphenols and phthalates were higher thanreported stormwater concentrations and European water quality standards. Pollutant sourceidentification and sustainable management of snow are important instruments for the mitigation oforganic contaminants in the urban environment.
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| 28. |
- Björklund, Karin, 1979, et al.
(författare)
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Screening of organic contaminants in urban snow
- 2010
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Ingår i: Proceedings, 14th International Conference, IWA Diffuse Pollution Specialist Group: Diffuse Pollution and Eutrophication. ; , s. 270-275
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Konferensbidrag (refereegranskat)abstract
- Snowmelt is known to cause peak concentrations of pollutants which may adversely affect receiving water quality. High concentrations in snow have been shown for e.g. metals and suspended solids, whereas studies on organic pollutants are rarely reported. This study aimsat investigating the occurrence of anthropogenic organic compounds in urban snow, and at identifying sources of the pollutants. Snow from sites in Gothenburg, Sweden, was sampled and a range of organic substances was analysed. The most frequently detected organic pollutants in urban snow were polycyclic aromatic hydrocarbons, high molecular-weightphthalates, 4-nonylphenol and 4-octylphenol. Brominated flame retardants and chlorinated paraffins were only sporadically detected. In several snow samples, the concentrations of specific PAHs, alkylphenols and phthalates were higher than reported stormwater concentrations and European water quality standards. Pollutant source identification and sustainable management of snow are important instruments for the mitigation of organic contaminants in the urban environment.
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| 29. |
- Björklund, Karin, 1979, et al.
(författare)
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Simulating organic pollutant flows in urban stormwater: development and evaluation of a model for nonylphenols and phthalates
- 2011
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Ingår i: Water Science and Technology. - : IWA Publishing. - 0273-1223 .- 1996-9732. ; 65:3, s. 508-515
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Tidskriftsartikel (refereegranskat)abstract
- Stormwater-quality models can be useful tools for predicting pollutant loads and identifying sources of contamination. Most models in current use handle pollutants such as metals, nutrients and suspended solids, whereas models including emerging organic contaminants are rare.This study aims at developing and evaluating a model for simulating stormwater flows of two groups of organic pollutants; nonylphenols and phthalates. Sources, emission patterns and environmental fate were examined to create a model framework for the organic contaminants. The model was calibrated using field data from three urban catchments. The results show that the simulated pollutant concentrations are overestimated compared to the measured concentrations, which are often close to or below the analytical detection limit. The high uncertainty and the lowpredictive power of the model may be explained by factors such as incorrect catchment data, lack of knowledge on buildup, washoff and other processes involved in substance fate, and an underreporting of pollutant concentrations in stormwater. More data on release patterns and sewer fate are needed to adequately simulate stormwater concentrations of nonylphenols and phthalates. A conventional substance flow analysis based on bookkeeping, evaluated in parallel to the computer model, has proven to be useful for calculating fluxes of nonylphenols and phthalates in urban catchments.
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| 30. |
- Björklund, Karin, 1979, et al.
(författare)
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Using the stormwater-quality model SEWSYS to identify sources and fluxes of hazardous organic substances
- 2008
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Ingår i: Book of abstracts. 9th Highway and Urban Environment Symposium, 9-11 June 2008, Madrid, Spain. ; , s. 21-
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Konferensbidrag (refereegranskat)abstract
- Stormwater-quality models simulating pollutant pathways in urban catchments are proven to be valuable means when planning and evaluating appropriate strategies to reduce the detrimental impacts of urban runoff [Zoppou, 2001]. SEWSYS, a substance flow model simulating pollutant concentrations in stormwater, is an effective instrument for predicting pollution loads from non-point sources and can therefore be used for proposing measures to reduce the release of contaminants into the environment [Ahlman, 2006]. SEWSYS is now being expanded from operating with nutrients and metals, to include also a few organic substances. In this study, two groups of organic compounds – phthalates and nonylphenols (NPs) – were selected for an initial flow analysis. These compounds, classified as “very toxic to aquatic organisms” [KEMI, 2007a], are high volume chemicals used as additives in a variety of products [KEMI, 2007b]. To incorporate phthalates and NPs into SEWSYS, an extensive literature study was accomplished. The study included identification of potential pollutant sources in urban areas, e.g. building materials and vehicles, and analysis of emission patterns and rates. Also, model input data such as source distribution and authentic rain series were collected from three urban catchment areas. Simulated pollutant concentrations were then compared with measured and analysed concentrations in stormwater from the investigated catchments. Preliminary model simulations showed that pollutant loadings and major contributing sources are catchment dependent: e.g. vehicles are the dominant sources of phthalates in traffic areas whereas construction materials are the most important sources in residential areas. These simulation results were then used for proposing adequate measures in order to mitigate phthalate and NP loads in stormwater. Calculations showed that by using phthalate- or NP-free construction materials, and by reducing the traffic load, the pollution load from residential and traffic areas was considerably decreased. These findings illustrate the use of SEWSYS as a tool for improving urban runoff quality, and corresponding simulations could easily be performed by local authorities for planning of effective abatement strategies. ReferencesAhlman S (2006) Modelling of Substance Flows in Urban Drainage Systems. Doctoral Thesis, Department of Civil and Environmental Engineering, Chalmers University of Technology, Göteborg, Sweden.KEMI (Swedish Chemicals Agency). (2007a) Classification List. http://apps.kemi.se/klassificeringslistan/default.cfmKEMI (Swedish Chemicals Agency). (2007b) KemI-Stat. http://apps.kemi.se/kemistat/start.aspx?sprak=eZoppou C (2001) Review of urban storm water models. Environmental Modelling & Software 16 (3), 195-231.
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| 31. |
- Björklund, Mats, et al.
(författare)
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Environmental Fluctuations and Level of Density-Compensation Strongly Affects the Probability of Fixation and Fixation Times
- 2011
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Ingår i: Bulletin of Mathematical Biology. - : Springer Science and Business Media LLC. - 0092-8240 .- 1522-9602. ; 73:7, s. 1666-1681
-
Tidskriftsartikel (refereegranskat)abstract
- The probability of, and time to, fixation of a mutation in a population has traditionally been studied by the classic Wright-Fisher model where population size is constant. Recent theoretical expansions have covered fluctuating populations in various ways but have not incorporated models of how the environment fluctuates in combination with different levels of density-compensation affecting fecundity. We tested the hypothesis that the probability of, and time to, fixation of neutral, advantageous and deleterious mutations is dependent on how the environment fluctuates over time, and on the level of density-compensation. We found that fixation probabilities and times were dependent on the pattern of autocorrelation of carrying capacity over time and interacted with density-compensation. The pattern found was most pronounced at small population sizes. The patterns differed greatly depending on whether the mutation was neutral, advantageous, or disadvantageous. The results indicate that the degree of mismatch between carrying capacity and population size is a key factor, rather than population size per se, and that effective population sizes can be very low also when the census population size is far above the carrying capacity. This study highlights the need for explicit population dynamic models and models for environmental fluctuations for the understanding of the dynamics of genes in populations.
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| 32. |
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| 33. |
- Björklund, Mats, et al.
(författare)
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Quantitative Trait Evolution and Environmental Change
- 2009
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Ingår i: PloS one. - : Public Library of Science (PLoS). - 1932-6203. ; 4:2, s. e4521-
-
Tidskriftsartikel (refereegranskat)abstract
- Background: Given the recent changes in climate, there is an urgent need to understand the evolutionary ability of populations to respond to these changes. Methodology/Principal Findings: We performed individual-based simulations with different shapes of the fitness curve, different heritabilities, different levels of density compensation, and different autocorrelation of environmental noise imposed on an environmental trend to study the ability of a population to adapt to changing conditions. The main finding is that when there is a positive autocorrelation of environmental noise, the outcome of the evolutionary process is much more unpredictable compared to when the noise has no autocorrelation. In addition, we found that strong selection resulted in a higher load, and more extinctions, and that this was most pronounced when heritability was low. The level of density-compensation was important in determining the variance in load when there was strong selection, and when genetic variance was lower when the level of density-compensation was low. Conclusions: The strong effect of the details of the environmental fluctuations makes predictions concerning the evolutionary future of populations very hard to make. In addition, to be able to make good predictions we need information on heritability, fitness functions and levels of density compensation. The results strongly suggest that patterns of environmental noise must be incorporated in future models of environmental change, such as global warming.
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| 34. |
- Björklund, Per
(författare)
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Aspects on the functional characteristics of the Roux-limb after Gastric Bypass surgery
- 2017
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Background: Bariatric surgery, and particularly the Roux-en-Y Gastric Bypass (RYGBP) procedure, has highlighted the central role of the gastrointestinal tract in the regulation of body weight and metabolism. The Roux-limb is the part of the small intestine that has become the food recipient after RYGBP and has generally been very little studied. The thesis explores mucosal adaptation and muscular activity of this intestinal segment after RYGBP. As there is an increasing awareness of post-surgical chronic abdominal pain and use of opioid analgesics in some patients, the motor patterns in the roux-limb in these conditions were examined as well. Paper I compares the mucosa of the preoperative jejunum with the same region in the Roux-limb 6-8 months post-surgery. In the Roux limb, the mucosal villi were flattened with an upregulated epithelial replication rate. No histological signs of inflammation were present, but there was increased expression of proinflammatory markers. In conclusion, after RYGBP the jejunal mucosa changes into an appearance more suited for food reception ⁄ transportation and tissue defence. Paper II. Mechano-sensory properties of the Roux limb in relation to food intake were exam-ined preoperatively, 6 weeks and 1 year after RYGBP. In general, the preferred meal size de-creased after RYGBP and subjects with a low perception threshold in the Roux limb preferred larger meals. Furthermore, the intra-Roux limb pressure correlated negatively to preferred meal size. The results suggest that the Roux limb is determinant for the regulation of food intake after RYGBP. Paper III. The motor activity of the gastric pouch and the proximal Roux limb during fasting and food intake was examined by use of high resolution manometry in patients after un-complicated RYGBP. A fasting motility pattern with migrating motility complexes (MMC) starting in the proximal Roux limb was characterised. Food ingestion was associated with a modest intraluminal pressure increase of similar magnitude in the gastric pouch and the Roux limb indicating that the gastric pouch and the Roux limb behave as a common cavity. Paper IV. In a retrospective analysis of 18 patients with chronic abdominal pain after RYGBP referred to high resolution manometry it was found that only 5/17 were evaluated as normal manometries, but there was no clear association to experienced symptoms. Out of the 18 pa-tients, 13 had longstanding opioid medication suggesting a potential element of narcotic bowel syndrome. In an attempt to elucidate opioid influence on Roux limb motility asymptomatic RYGBP patients was administered morphine intravenously during manometry. The MMC-pattern did not change but the muscular tone increased during morphine infusion. The fact morphine influenced the motor behaviour of the Roux-limb support a role for opioid treatment in causing chronic abdominal pain after RYGBP surgery. However, the links between muscular activity and symptoms remain to be investigated. Conclusion: This thesis project demonstrates that biomechanical properties and muscular ac-tivity of the Roux-limb can be clinically important, and particularly so during dysfunctional conditions.
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| 35. |
- Björklund, Peyman, et al.
(författare)
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Culture of Parathyroid Cells
- 2012
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Ingår i: Human Cell Culture Protocols. - Totowa, NJ : Springer. - 9781617793660 ; , s. 43-53
-
Bokkapitel (refereegranskat)abstract
- The parathyroid cells are highly differentiated with more or less their only function to secrete parathyroid hormone in response to the extracellular calcium level. Tumours from the parathyroid glands are >99% benign, and have a slow proliferation rate. Culture of parathyroid cells is known to be very difficult most likely due to the high differentiation level. This chapter reveals some details in order how to get parathyroid cells to survive in culture after dispersion of normal bovine parathyroid glands or pathological human parathyroid tumours. Detailed protocols describing cell dispersion with collagenase, short-term cultures, and establishment of long-term cultures are presented.
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| 36. |
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| 37. |
- Björklund, Per, et al.
(författare)
-
Is the roux limb a determinant for meal size after gastric bypass surgery?
- 2010
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Ingår i: Obesity surgery. - : Springer Science and Business Media LLC. - 1708-0428 .- 0960-8923. ; 20:10, s. 1408-14
-
Tidskriftsartikel (refereegranskat)abstract
- The Roux-Y gastric bypass (RYGBP) is an effective weight-reducing procedure but the involved mechanisms of action are obscure. The Roux limb is the intestinal segment that following surgery is the primary recipient for food intake. The aims of the study were to explore the mechanosensory and biomechanical properties of the Roux limb and to make correlations with preferred meal size. Ten patients participated and were examined preoperatively, 6weeks and 1year after RYGBP. Each subject ingested unrestricted amounts of a standardized meal and the weight of the meal was recorded. On another study day, the Roux limb was subjected to gradual distension by the use of an intraluminal balloon. Luminal volume-pressure relationships and thresholds for induction of sensations were monitored. At 6weeks and 1year post surgery, the subjects had reduced their meal sizes by 62% and 41% (medians), respectively, compared to preoperative values. The thresholds for eliciting distension-induced sensations were strongly and negatively correlated to the preferred meal size. Intraluminal pressure during Roux limb distension, both at low and high balloon volumes, correlated negatively to the size of the meal that the patients had chosen to eat. The results suggest that the Roux limb is an important determinant for regulating food intake after Roux-Y bypass bariatric surgery.
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| 38. |
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| 39. |
- Björklund, Per, et al.
(författare)
-
Roux Limb Motility in Gastric Bypass Patients with Chronic Abdominal Pain—Is There an Association to Prescribed Opioids?
- 2019
-
Ingår i: Obesity Surgery. - : Springer Science and Business Media LLC. - 0960-8923 .- 1708-0428. ; 29:12, s. 3860-3867
-
Tidskriftsartikel (refereegranskat)abstract
- Background/Aim: A number of patients continue to suffer from chronic abdominal pain of unknown origin, which may also lead to a prolonged use of opioid analgesics. Symptoms of abdominal pain, nausea and vomiting in this patient group resemble the characteristics of the Roux stasis Syndrome. The aim was to elucidate relationships between chronic abdominal pain, Roux limb motor activity and opioid analgesics. Methods: Roux limb high-resolution manometry and ratings of abdominal pain and quality of life were analysed in 15 gastric bypass patients reporting abdominal pain of unknown origin. Effect of acute opiate administration (morphine i.v.) on fasting Roux limb motor activity was assessed in asymptomatic and morphine-naïve gastric bypass patients (n = 9) and compared with an untreated control group (n = 11). Results: In the symptomatic patient group, we found disturbed Roux limb motor patterns in 10 out of 15 examinations, but no signs of Roux stasis syndrome. A high prevalence of prescribed opioid analgesics as well as a high number of reoperations in this group. The worst quality of life and the highest number of pain-killing medications were observed among the patients with distal pacemaker activity in Roux limb. In the morphine-naïve and asymptomatic patients, morphine increased the muscular tone in the Roux limb during phase III-like motor activity. Summary and Conclusions: A majority of the RYGBP patients with chronic abdominal pain had a disturbed Roux limb fasting motility, and there was a high prevalence of prescribed opioid analgesics. In opiate-naïve RYGBP patients, acute morphine intravenously increased the muscular tone of the Roux limb. © 2019, The Author(s).
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| 40. |
- Björklund, Peyman, et al.
(författare)
-
Stathmin as a Marker for Malignancy in Pheochromocytomas
- 2010
-
Ingår i: Experimental and clinical endocrinology & diabetes. - : Georg Thieme Verlag KG. - 0947-7349 .- 1439-3646. ; 118:1, s. 27-30
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Tidskriftsartikel (refereegranskat)abstract
- Pheochromocytomas of the adrenal medulla may be life-threatening catecholamine-producing tumors which are malignant in about 10% of cases. Differential diagnosis between malignant and benign tumors is dependent on the development of metastasis or extensive local invasion. A number of genetic aberrations have been described in pheochromocytomas, but no marker associated to malignancy has been reported. We applied an expression microarray containing 7770 cDNA clones and analysed the expression profiles in eleven tumors compared to normal adrenal medulla. Stathmin (STMN1, Op18) was most conspiciously overexpressed among the differentially expressed genes. RT-PCR analysis further confirmed mRNA overexpression, 6 to 8-fold for benign and malignant tumors, and 16-fold for metastases. Stathmin protein overexpression was observed by immunohistochemistry, and distinct differential protein expression between benign and malignant/metastasis specimens was confirmed by Western blot analysis. The results introduce stathmin as a possible diagnostic marker for malignant pheochromocytomas, and further evaluations are warranted.
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| 41. |
- Björklund, Per, et al.
(författare)
-
The pros and cons of gastric bypass surgery – The role of the Roux-limb
- 2019
-
Ingår i: Best Practice and Research: Clinical Gastroenterology. - : Elsevier BV. - 1521-6918. ; 40-41
-
Tidskriftsartikel (refereegranskat)abstract
- The prevalence of overweight and obesity has exploded in the post-industrial era. Life style interventions like dieting and exercise can induce a marked weight loss, but the main problem for most patients is to maintain the reduced body weight over time. Gastric bypass surgery is a commonly performed and very effective method for achieving a pronounced and sustained weight loss including metabolic improvements in obese patients. Despite the therapeutic successfulness there are known side-effects like chronic postprandial nausea and pain that in some patients become intractable. The pathophysiology is complex and partly unexplored. The physician or surgeon handling a patient with “post-bariatric symptoms” must be aware of the risk for symptom aggravations due to iatrogenic opioid-associated intestinal dysmotility. The present paper gives a brief overview of obesity surgery and its associated postsurgical conditions with a focus on the unexplored role of the Roux-limb following gastric bypass surgery. © 2019 Elsevier Ltd
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| 42. |
- Björklund, Svante, et al.
(författare)
-
Measurement of Rank and Other Properties of Direct and Scattered Signals
- 2016
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Ingår i: International Journal of Antennas and Propagation. - : Hindawi Publishing Corporation. - 1687-5869 .- 1687-5877. ; 2016
-
Tidskriftsartikel (refereegranskat)abstract
- We have designed an experiment for low-cost indoor measurements of rank and other properties of direct and scattered signals with radar interference suppression in mind. The signal rank is important also in many other applications, for example, DOA (Direction of Arrival) estimation, estimation of the number of and location of transmitters in electronic warfare, and increasing the capacity in wireless communications. In real radar applications, such measurements can be very expensive, for example, involving airborne radars with array antennas. We have performed the measurements in an anechoic chamber with several transmitters, a receiving array antenna, and a moving reflector. Our experiment takes several aspects into account: transmitted signals with different correlation, decorrelation of the signals during the acquisition interval, covariance matrix estimation, noise eigenvalue spread, calibration, near-field compensation, scattering in a rough surface, and good control of the influencing factors. With our measurements we have observed rank, DOA spectrum, and eigenpatterns of direct and scattered signals. The agreement of our measured properties with theoretic and simulated results in the literature shows that our experiment is realistic and sound. The detailed description of our experiment could serve as help for conducting other well-controlled experiments. © Copyright 2016 Svante Björklund et al.
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| 43. |
- Björklund, Svante, et al.
(författare)
-
Radar-Like Measurements with an Experimental Digital Beamforming Array Antenna
- 1998
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Konferensbidrag (refereegranskat)abstract
- An experimental S-band digital beamforming receiving array antenna has been used in “radar- like” measurements, where radar signals with programmable waveforms are transmitted. The measurements are carried out in an anechoic chamber and the measured signals come from the direct path from transmitter antennas to the receiver antenna. In this way targets are simulated. On the received signals, conventional beamforming, pulse compression, doppler filtering and high resolution direction of arrival estimation are performed. Accurate channel calibration and channel equalization are utilized. This paper first describes the receiving antenna, transmitter control, measurement arrangements, calibration and signal processing. It then presents some measurement results for high resolution direction of arrival estimation and for radar resolution of one and two targets.
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| 44. |
- Block, Linda, et al.
(författare)
-
Ultralow concentrations of bupivacaine exert anti-inflammatory effects on inflammation-reactive astrocytes.
- 2013
-
Ingår i: The European journal of neuroscience. - : Wiley. - 1460-9568 .- 0953-816X. ; 38:11, s. 3669-3678
-
Tidskriftsartikel (refereegranskat)abstract
- Bupivacaine is a widely used, local anesthetic agent that blocks voltage-gated Na(+) channels when used for neuro-axial blockades. Much lower concentrations of bupivacaine than in normal clinical use, <10(-8) m, evoked Ca(2+) transients in astrocytes from rat cerebral cortex, that were inositol trisphosphate receptor-dependent. We investigated whether bupivacaine exerts an influence on the Ca(2+) signaling and interleukin-1β (IL-1β) secretion in inflammation-reactive astrocytes when used at ultralow concentrations, <10(-8) m. Furthermore, we wanted to determine if bupivacaine interacts with the opioid-, 5-hydroxytryptamine- (5-HT) and glutamate-receptor systems. With respect to the μ-opioid- and 5-HT-receptor systems, bupivacaine restored the inflammation-reactive astrocytes to their normal non-inflammatory levels. With respect to the glutamate-receptor system, bupivacaine, in combination with an ultralow concentration of the μ-opioid receptor antagonist naloxone and μ-opioid receptor agonists, restored the inflammation-reactive astrocytes to their normal non-inflammatory levels. Ultralow concentrations of bupivacaine attenuated the inflammation-induced upregulation of IL-1β secretion. The results indicate that bupivacaine interacts with the opioid-, 5-HT- and glutamate-receptor systems by affecting Ca(2+) signaling and IL-1β release in inflammation-reactive astrocytes. These results suggest that bupivacaine may be used at ultralow concentrations as an anti-inflammatory drug, either alone or in combination with opioid agonists and ultralow concentrations of an opioid antagonist.
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| 45. |
- Brundin, Patrik, et al.
(författare)
-
Bilateral caudate and putamen grafts of embryonic mesencephalic tissue treated with lazaroids in Parkinson's disease
- 2000
-
Ingår i: Brain. - 1460-2156. ; 123, s. 1380-1390
-
Tidskriftsartikel (refereegranskat)abstract
- Five parkinsonian patients were transplanted bilaterally into the putamen and caudate nucleus with human embryonic mesencephalic tissue from between seven and nine donors. To increase graft survival, the lipid peroxidation inhibitor tirilazad mesylate was administered to the tissue before implantation and intravenously to the patients for 3 days thereafter. During the second postoperative year, the mean daily L-dopa dose was reduced by 54% and the UPDRS (Unified Parkinson's Disease Rating Scale) motor score in 'off' phase was reduced by a mean of 40%. At 10-23 months after grafting, PET showed a mean 61% increase of 6-L-[(18)F]fluorodopa uptake in the putamen, and 24% increase in the caudate nucleus, compared with preoperative values. No obvious differences in the pattern of motor recovery were observed between these and other previously studied cases with putamen grafts alone. The amount of mesencephalic tissue implanted in each putamen and caudate nucleus was 42 and 50% lower, respectively, compared with previously transplanted patients from our centre. Despite this reduction in grafted tissue, the magnitudes of symptomatic relief and graft survival were very similar. These findings suggest that tirilazad mesylate may improve survival of grafted dopamine neurons in patients, which is in agreement with observations in experimental animals.
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| 46. |
- Börgeson, Emma, et al.
(författare)
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AICAR ameliorates high-fat diet-associated pathophysiology in mouse and ex vivo models, independent of adiponectin.
- 2017
-
Ingår i: Diabetologia. - : Springer Science and Business Media LLC. - 1432-0428 .- 0012-186X. ; 60:4, s. 729-739
-
Tidskriftsartikel (refereegranskat)abstract
- In this study, we aimed to evaluate the therapeutic potential of 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), an activator of AMP-activated protein kinase, for ameliorating high-fat diet (HFD)-induced pathophysiology in mice. We also aimed to determine whether the beneficial effects of AICAR were dependent on adiponectin. Furthermore, human adipose tissue was used to examine the effect of AICAR ex vivo.Six-week-old male C57BL/6J wild-type and Adipoq (-/-) mice were fed a standard-fat diet (10% fat) or an HFD (60% fat) for 12weeks and given vehicle or AICAR (500μg/g) three times/week from weeks 4-12. Diet-induced pathophysiology was examined in mice after 11 weeks by IPGTT and after 12 weeks by flow cytometry and western blotting. Human adipose tissue biopsies from obese (BMI 35-50kg/m(2)) individuals were incubated with vehicle or AICAR (1mmol/l) for 6h at 37°C, after which inflammation was characterised by ELISA (TNF-α) and flow cytometry.AICAR attenuated adipose inflammation in mice fed an HFD, promoting an M1-to-M2 macrophage phenotype switch, while reducing infiltration of CD8(+) T cells. AICAR treatment of mice fed an HFD partially restored glucose tolerance and attenuated hepatic steatosis and kidney disease, as evidenced by reduced albuminuria (p<0.05), urinary H2O2 (p<0.05) and renal superoxide levels (p<0.01) in both wild-type and Adipoq (-/-) mice. AICAR-mediated protection occurred independently of adiponectin, as similar protection was observed in wild-type and Adipoq (-/-) mice. In addition, AICAR promoted an M1-to-M2 macrophage phenotype switch and reduced TNF-α production in tissue explants from obese human patients.AICAR may promote metabolic health and protect against obesity-induced systemic diseases in an adiponectin-independent manner. Furthermore, AICAR reduced inflammation in human adipose tissue explants, suggesting by proof-of-principle that the drug may reduce obesity-induced complications in humans.ClinicalTrials.gov NCT02322073.
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| 47. |
- Casely-Hayford, Jeffrey, et al.
(författare)
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Enculturating a Protective Professional Community - Processes of Teacher Retention in a Swedish Hard-to-Staff School
- 2024
-
Ingår i: Education Sciences. - : MDPI. - 2227-7102. ; 14:1
-
Tidskriftsartikel (refereegranskat)abstract
- This study presented a positive deviant case: a Swedish hard-to-staff school which has had a low rate of teacher turnover over time. In line with the purpose of studying positive deviance in organisations, our exploratory inquiry was geared towards understanding how and why 'at-risk' teachers, i.e., teachers who teach in subjects which are known to have high levels of staffing difficulties in Sweden, stayed at this particular school. Using a modified grounded theory approach, our results suggested that teachers remained at the school due to being embedded in a protective professional community that was enculturated by different expressions of collegiality. Finally, these findings are discussed in relation to the theoretical concepts of teachers' job embeddedness and social capital.
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| 48. |
- Casely-Hayford, Jeffrey, et al.
(författare)
-
What makes teachers stay? : A cross-sectional exploration of the individual and contextual factors associated with teacher retention in Sweden.
- 2022
-
Ingår i: Teaching and Teacher Education. - : Elsevier. - 0742-051X .- 1879-2480. ; 113
-
Tidskriftsartikel (refereegranskat)abstract
- This cross-sectional study aimed to identify individual and contextual factors associated with Swedish teachers' intention to remain in the profession. A sample of 5903 elementary-year teachers completed a questionnaire between 2004 and 2011 assessing their health and perception of individual and contextual factors at work. The findings showed that teachers' intention to remain in the profession is mainly explained by individual factors, predominantly by teachers' perceived health state, work motivation and collegial support. The main implication is the importance of teachers' perceived health state for their intention to remain, underscoring the importance of a health promoting work environment in schools. (c) 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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| 49. |
- Cervin, Jakob, et al.
(författare)
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Fucose-Galactose Polymers Inhibit Cholera Toxin Binding to Fucosylated Structures and Galactose-Dependent Intoxication of Human Enteroids.
- 2020
-
Ingår i: ACS infectious diseases. - : American Chemical Society (ACS). - 2373-8227. ; 6:5, s. 1192-1203
-
Tidskriftsartikel (refereegranskat)abstract
- A promising strategy to limit cholera severity involves blockers mimicking the canonical cholera toxin ligand (CT) ganglioside GM1. However, to date the efficacies of most of these blockers have been evaluated in noncellular systems that lack ligands other than GM1. Importantly, the CT B subunit (CTB) has a noncanonical site that binds fucosylated structures, which in contrast to GM1 are highly expressed in the human intestine. Here we evaluate the capacity of norbornene polymers displaying galactose and/or fucose to block CTB binding to immobilized protein-linked glycan structures and also to primary human and murine small intestine epithelial cells (SI ECs). We show that the binding of CTB to human SI ECs is largely dependent on the noncanonical binding site, and interference with the canonical site has a limited effect while the opposite is observed with murine SI ECs. The galactose-fucose polymer blocks binding to fucosylated glycans but not to GM1. However, the preincubation of CT with the galactose-fucose polymer only partially blocks toxic effects on cultured human enteroid cells, while preincubation with GM1 completely blocks CT-mediated secretion. Our results support a model whereby the binding of fucose to the noncanonical site places CT in close proximity to scarcely expressed galactose receptors such as GM1 to enable binding via the canonical site leading to CT internalization and intoxication. Our finding also highlights the importance of complementing CTB binding studies with functional intoxication studies when assessing the efficacy inhibitors of CT.
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| 50. |
- Choi, Murim, et al.
(författare)
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K+ Channel Mutations in Adrenal Aldosterone-Producing Adenomas and Hereditary Hypertension
- 2011
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 0036-8075 .- 1095-9203. ; 331:6018, s. 768-772
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Tidskriftsartikel (refereegranskat)abstract
- Endocrine tumors such as aldosterone-producing adrenal adenomas (APAs), a cause of severe hypertension, feature constitutive hormone production and unrestrained cell proliferation; the mechanisms linking these events are unknown. We identify two recurrent somatic mutations in and near the selectivity filter of the potassium (K+) channel KCNJ5 that are present in 8 of 22 human APAs studied. Both produce increased sodium (Na+) conductance and cell depolarization, which in adrenal glomerulosa cells produces calcium (Ca2+) entry, the signal for aldosterone production and cell proliferation. Similarly, we identify an inherited KCNJ5 mutation that produces increased Na+ conductance in a Mendelian form of severe aldosteronism and massive bilateral adrenal hyperplasia. These findings explain pathogenesis in a subset of patients with severe hypertension and implicate loss of K+ channel selectivity in constitutive cell proliferation and hormone production.
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